WO2004014145A1 - Animal feed material and animal feed using the material - Google Patents

Animal feed material and animal feed using the material Download PDF

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Publication number
WO2004014145A1
WO2004014145A1 PCT/JP2003/009773 JP0309773W WO2004014145A1 WO 2004014145 A1 WO2004014145 A1 WO 2004014145A1 JP 0309773 W JP0309773 W JP 0309773W WO 2004014145 A1 WO2004014145 A1 WO 2004014145A1
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WIPO (PCT)
Prior art keywords
animal feed
feed material
krill
material according
protein
Prior art date
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PCT/JP2003/009773
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French (fr)
Japanese (ja)
Inventor
Tetsu Mori
Yoshio Matsuda
Kazuhiro Yoshikawa
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Nippon Suisan Kaisha, Ltd.
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Publication date
Application filed by Nippon Suisan Kaisha, Ltd. filed Critical Nippon Suisan Kaisha, Ltd.
Priority to JP2004527319A priority Critical patent/JP4804003B2/en
Priority to CA2494945A priority patent/CA2494945C/en
Priority to AU2003252452A priority patent/AU2003252452B2/en
Publication of WO2004014145A1 publication Critical patent/WO2004014145A1/en
Priority to NO20050128A priority patent/NO20050128L/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Definitions

  • the present invention relates to an animal feed material containing a vegetable protein material such as soybean, soybean meal (defatted soybean), wheat, barley, corn dalnut meal, rice bran, and an animal feed material using the same.
  • a vegetable protein material such as soybean, soybean meal (defatted soybean), wheat, barley, corn dalnut meal, rice bran, and an animal feed material using the same.
  • the amino acid balance can be overcome by using multiple feed families, but the development of new technologies has been desired for the decomposition and removal of digestive absorption inhibitors such as indigestible carbohydrates and phytin (phytic acid).
  • digestive absorption inhibitors such as indigestible carbohydrates and phytin (phytic acid).
  • studies have been conducted to improve the digestibility of cultivated fish and shellfish feed by fermenting the koji mold Aspergillus sp.) On defatted soybean meal to enhance digestibility. Although it is inferior in growth performance, it has been reported that growth performance is improved as compared with the case of using unprocessed soybean meal (Non-Patent Document 1).
  • Patent Document 1 discloses a method of inoculating soybean meal with a microorganism and performing fermentation treatment to decompose and remove oligosaccharides such as sucrose, raffinose, and stachyose in the soybean meal.
  • the microorganism used in this case is Aspergillus ⁇ , > 3 ⁇ 4 ⁇ 300? ⁇ 5 genus, BacillusM, ac. Normal acid diet such as Z? Aci JiAs genus! It discloses that it can be made from fermented foods and fermented foods, and discloses that soybean meal that has been inoculated and fermented and administered to juvenile hamachi will improve growth.
  • carnivorous fish such as yellowtail and red sea bream decreases as the proportion of the vegetable protein material in the feed material increases. 2 In fact, it is necessary to add 5 to 10% of Okia meal to attract food.
  • Patent Document 2 discloses a feed for aquatic animals using a krill enzyme (krill enzyme).
  • the krill enzyme is mixed with feed while maintaining its activity.
  • the idea is that feeding krill enzymes along with feed helps digestion of animals. Since it must be purified and added to the feed while maintaining the activity of the krill enzyme, it cannot be heated.Therefore, there are many restrictions on its handling, making it difficult to use as a versatile feed material. is there.
  • Patent Literature 3 describes using autolyzed krill as a feed material. It is described that it is easily digested by a protease contained in krill.
  • Patent Literature 1 JP-A-5-26 8 8 8 1
  • Patent Document 2 Japanese Patent Application Laid-open No. Hei 8—24 2 7 7 7
  • Patent Document 3 WO 9 8/344 9 8
  • Non-Patent Document 1 Journal of the Fisheries Society of Japan, Vol. 59, No. 1, 8 8 3— 1 8 8 8 (1 9 9 3)
  • Non-Patent Document 2 Aoki, et al .: Suisanzoshoku, 48, 73-79, (2000) Disclosure of the Invention
  • the present invention uses planta proteins such as soybean, defatted soybean meal, wheat, corn dalten meal, rice bran, defatted rice bran, processed bran, oilseed rapeseed, cottonseed oilseed, and potato protein to enhance the digestibility of the protein,
  • the purpose of the present invention is to provide an animal feed material having an effect of inducing feeding and an animal feed made from the same.
  • the gist of the present invention is the following animal feed materials (1) to (8).
  • An animal feed material comprising a vegetable protein material that has been treated with a krill component that has not been subjected to autolysis.
  • the vegetable protein raw material is at least one vegetable protein raw material selected from the group consisting of soybean, defatted soybean meal, corn dalnut meal, raw bran, processed bran, wheat, rapeseed oil cake, cottonseed oil cake, and potato protein.
  • the animal feed material according to any one of the above (1) to (5).
  • the gist of the present invention is the following animal feed (10).
  • FIG. 1 is a drawing showing the action of kinako, soymilk and egg white having protease inhibitory action on krill enzyme.
  • the animal feed material in the present invention is a feed material used for livestock, poultry farming, and fish farming, and is a feed material used as a protein supplement source.
  • the feed of the present invention is particularly suitable for fish farming and crustacean farming.
  • fish culture, echinoderms and crustaceans include fish to be cultivated such as red sea bream, salmon, yellowtail, sardine, trout, flounder, tuna, penguin, squid and shrimp.
  • any vegetable protein material can be used as long as it is a vegetable protein material used as a substitute for animal protein material in animal feed materials.
  • the main ingredients are soybeans, defatted soybean meal, corn dalten meal, raw bran, processed bran, wheat, rapeseed oil cake, and cottonseed. Oil cake, potato protein and the like are exemplified.
  • the krill used in the present invention is preferably Antarctic krill, which has abundant resources and high enzyme activity, but other krills having the same activity can be used.
  • krill component besides krill or minced krill, krill endogenous enzymes selectively obtained by squeezing can be added to the vegetable protein material and matured.
  • the krill and compressed solution containing these krill endogenous enzymes must have an indegradable carbohydrate-decomposing activity, and the activity can be easily confirmed by thin-layer chromatography.
  • the degradation activity of the indigestible saccharide is measured as described below.
  • oligosaccharides such as cellobiose or maltohexaose are added to a final concentration of 1%, and the mixture is stirred at 40 for 2 hours. Perform processing. Thereafter, 3000 r PP m, 10 minutes, spotted supernatant 10 L obtained Te Gyotsu centrifuged at 4 ° C in silica force gel thin layer plates, butanol: propanol: 7K (1: 3: 2 ) And color development using orcinol sulfuric acid solution.
  • spots of oligosaccharides such as cellobiose and maltohexose are completely eliminated, and only those that have the activity of appearing spots of monosaccharides considered to be ⁇ -glucose, a-vulose, etc. Is done.
  • Animal feed ingredients of c present invention to use one confirming the activity in a similar manner with respect Okiami pressate is Okiami vegetable protein material containing indigestible saccharides, Okiami minced treated or pressate, Plus 2 Aging treatment is carried out at 0 to 60 ° C, preferably 30 to 50 ° C for 1 to 48 hours, preferably 2 to 6 hours, and then at 80 ° C or more, preferably 85 ° C, 1 It is obtained by performing heat sterilization while maintaining for 0 minutes and then subjecting it to drying. During ripening, stirring is performed, but it is also possible to add fresh water so that stirring is difficult and ripening is not insufficient. The reaction time needs to be adjusted depending on the amount of the enzyme relative to the vegetable protein. However, if the amount of free amino acids increases, the feed efficiency deteriorates. Animal feed using the animal feed material of the present invention as a raw material.
  • the animal feed of the present invention can be produced by processing and shaping the animal feed raw material of the present invention and other feed raw materials together.
  • the animal feed raw material of the present invention can be provided in powder form by heating and drying after krill treatment, and can be easily used as a raw material for various types of feed, particularly as a substitute for animal protein. Can be. In the case of fish farming, it can be used as a substitute for fish meal. Action
  • Protein digestibility is improved by adding krill having indigestible carbohydrate-decomposing activity, minced krill or krill squeezed solution to the indigestible carbohydrate-containing plant protein raw material and ripening it.
  • defatted soybean meal After mincing 25 kg of krill having indigestible carbohydrate-decomposing activity, 3 O kg of defatted soybean meal was added, and the mixture was heated and stirred at 40 ° C for 2 hours to produce degraded defatted soybean meal (hereinafter, referred to as “ Komi-minced soybean meal).
  • krill having indigestible carbohydrate-decomposing activity is squeezed to prepare a squeezed liquid (enzyme solution) having a weight of 10% of krill raw material, and 3 O kg of defatted soybean meal is added to the mixture at 40 ° C. Then, the mixture was heated and stirred for 2 hours to prepare decomposed defatted soybean meal (hereinafter referred to as krill enzyme solution-treated soybean meal).
  • krill-treated soybean meal egg white addition After mincing 25 kg of krill, which has indigestible carbohydrate-decomposing activity, add 250 g of egg white and 3 O kg of defatted soybean meal, and heat and stir for 40 or 2 hours to produce degraded defatted soybean meal. (Hereinafter referred to as krill-treated soybean meal egg white addition).
  • Red sea bream (average body weight: 17.9 g) was used as a test fish for a breeding experiment for 56 days.
  • Table 1 shows the composition of the breeding feed used, and Tables 2 and 3 show the breeding results. Composition of feed for red sea bream test (%)
  • Example 1 1st section 2nd section 3rd section 4th section
  • Example 3 At start 17.9 17.9 17.8 17.8 Rearing Week 8 60. 6 59. 5 61. 1 62.0 Weight gain ( g) 42. 7 41. 6 43. 2 44.2 Table 3
  • Table 4 shows the composition of the feed used for breeding, and Tables 5 and 6 show the breeding results.
  • the kaki mince-treated group (Example 1) and the krill enzyme solution-treated group (Example 3) showed lower weight gain (growth) than the control group to which no defatted soybean meal was added. It was effective in terms. In addition, it was more effective than the 9th section where only krill was mixed, and it was confirmed that treating defatted soybean meal with krill was effective. Furthermore, it was also found that the growth was inferior in the case of cellulase treatment (enzyme-treated section) which is usually commercially available, indicating the necessity of ripening treatment with krill.
  • the squeezed liquid prepared from krill caught in February 2002 was a ⁇ summer '' lot
  • the squeezed liquid prepared from krill caught in May 2002 was a ⁇ winter '' lot It is described. This is a name that reflects the seasons in the Antarctic Ocean where the fish are harvested.
  • Kinase powder (roasted soybean powder) and egg white powder were each prepared at a concentration of 12% (w / v).
  • distilled water, kinako suspension, soy milk (solid content 12% w / v), or egg white suspension The suspension was added at 0.1 mL.
  • the content of kinako, soymilk, or egg white is calculated to be 0.4% in solid content.
  • the protease activity and carbohydrate-degrading enzyme activity of the krill squeezed solution added were measured.
  • the activity (increase in absorbance) when distilled water (immediately without solids) was added was set to 100%, and the activity when each additive was added was calculated.
  • the present invention can provide an animal feed raw material using a vegetable protein, in which digestibility of the protein is improved and which has an effect of inducing food intake. .
  • the present invention can provide an animal feed that can significantly reduce the use ratio of animal protein raw materials using the animal feed raw materials as raw materials.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
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Abstract

It is intended to provide a useful animal feed material capable of enhancing the digestibility of a vegetable protein material, which is valuable as an alternative protein source for animal protein materials, and an animal feed produced using the same. A vegetable protein material containing a hardly digestible carbohydrate such as soybean, defatted soybean cake, wheat, corn gluten meal, rice bran, defatted rice bran, processed bran, rapeseed oil cake, cottonseed oil cake or potato protein is treated with krill or an endoenzyme of krill to thereby partially digest the hardly digestible carbohydrate. Thus, a feed material having an enhanced digestibility is provided. Also, an animal feed with the use of the feed material is provided.

Description

明 細 書 動物用飼料原料及びそれを原料とする動物用飼料 技術分野  Description Animal feed raw materials and animal feeds derived from them
本発明は、 大豆、 大豆粕 （脱脂大豆） 、 小麦、 大麦、 コーンダルテン ミール、 米糠など植物性タンパク質原料を含む動物用飼料原料及びそれ を原料とする動物用飼料に関する。 背景技術  The present invention relates to an animal feed material containing a vegetable protein material such as soybean, soybean meal (defatted soybean), wheat, barley, corn dalnut meal, rice bran, and an animal feed material using the same. Background art
畜産物ならびに養殖魚介類は、 ヒ トにとって重要なタンパク質供給源 であることは周知のとおりであり、 それらを飼育 ·養殖するための飼料 は多くの分野で古くから研究が行われ、 効率の良い飼料原料が開発され てきた。 畜産用原料では大豆粕が供給安定であり、 かつ安価なため注目 されているものの、 少糖類や消化酵素阻害物質、 吸収阻害物質、 成長阻 害物質などが含まれているためにその利用が伸び悩んでいるのが実態で ある。 そのため、 アルコール洗浄や酸洗浄により不要成分を除去して良 質の飼料を提供する手法や、 あるいはカ ビ類 (^ Aspergillus氯、 Rhizopus ) や ( SaccharomycesM ) 、 糸田 ¾ H ( SCJゾ 〃 ·員、 Lac tobacillus^ ) などを単独、 あるいは 2種以上の組合せで接種発酵 して消化吸収性の良い飼料原料を製造したり、 動物飼料原料中の不要成 分除去法として、 糖質あるいは少糖類分解酵素であるセルラーゼ、 ガラ クタナーゼ、 ラムノガラクチュロナ一ゼゃ、 フィチン （フィチン酸） 分 解酵素であるフィターゼを添加して分解除去するとともに、 さらに消化 性を高めるためにタンパク質分解酵素を添加することが行われている。 これらの分解酵素もやはりカビ類 Aspergillus 、 Rhizopus ) ゃ酵 母類 ( Saccharomyces i ) 、 糸田菌類 (Bacillus ^ Lac tobacillus^^ な どに由来するものであり、 これら酵素の供給源となる微生物を培養する 力 あるいは遺伝子組換えによる生産によって必要とする酵素を提供す るものである。 しかしながら、 これらの技術ではカビ類、 酵母類、 細菌 類など微生物の予備培養が必要で、 微生物的な管理を徹底する必要があ るため、 全工程を考慮すると更に簡便化する必要がある。 It is well known that livestock products and cultured fish and shellfish are important protein sources for humans, and feed for breeding and cultivating them has been studied in many fields for a long time, and is efficient. Feed ingredients have been developed. As a raw material for livestock, soybean meal is attracting attention because its supply is stable and inexpensive, but its use is sluggish because it contains oligosaccharides, digestive enzyme inhibitors, absorption inhibitors, growth inhibitors, etc. The fact is that For this reason, a method of providing high-quality feed by removing unnecessary components by alcohol washing or acid washing, or molds (^ Aspergillus 氯, Rhizopus), (SaccharomycesM), Itoda H (SCJ zo Lac tobacillus ^) alone or in combination with two or more types to produce a feed material with good digestibility and absorbability, and as a method for removing unnecessary components from animal feed material, use carbohydrate or oligosaccharide degrading enzymes. Cellulase, galactanase, rhamnogalacturonase ゃ, and phytase (phytic acid) degrading enzyme, to decompose and remove them, and to add proteolytic enzymes to further enhance digestibility. Has been done. These degradative enzymes are also molds Aspergillus, Rhizopus) It is derived from the mother (Saccharomyces i), Itoda fungi (Bacillus ^ Lac tobacillus ^^), etc., and provides the enzyme required for culturing microorganisms as a source of these enzymes or for production by genetic recombination. However, these technologies require pre-culture of microorganisms such as molds, yeasts, and bacteria, and require thorough microbial control, which makes the process even easier if all processes are taken into account. There is a need to.
一方、 魚介類の蓄養殖産業は国際的にも益々盛んになりつつあり、 飼 料タンパク質源である沿岸魚粉の需要が著しく高まっているが、 沿岸魚 の漁獲量の変動が著しいため、 生産者は魚粉の価格変動に苦しんでいる のが実情である。 こういった中で、 以前より魚粉代替タンパク質として 植物性タンパク質、 特に脱脂大豆粕が注目されてきたが、 蓄養殖魚介類 の必要ァミノ酸が、 植物性タンパク質の構成ァミノ酸と異なるためァミ ノ酸バランスが崩れること、 消化酵素阻害活性因子が含まれていること、 植物の難消化性糖質が消化吸収を阻害することなど、 その添加量には限 界があった。 アミノ酸バランスに関しては複数の飼料原科を用いること によって克服できるが、 難消化性糖質、 フィチン （フィチン酸） などの 消化吸収阻害物質の分解除去には新たな技術の開発が望まれていた。 このよ う な背景から、 蓄養殖魚介類の飼料においても麹菌類 Aspergillus属、 ) を脱脂大豆粕に作用させて発酵し、 消化性を高め る研究がなされており、 大豆粕無添加の場合に比べると成長性は劣るも のの、 無加工の大豆粕を用いた場合よりは成長性が改善されると報告さ れている （非特許文献 1 ) 。  On the other hand, the seafood aquaculture industry is becoming more and more active internationally, and the demand for coastal fish meal, which is a source of feed protein, has been increasing significantly. Is currently suffering from fluctuations in fish meal prices. Under these circumstances, vegetable protein, especially defatted soybean meal, has been attracting attention as an alternative protein to fish meal. However, amino acids required for farmed fish and shellfish are different from the amino acids that make up vegetable proteins. There was a limit to the amount of acid added, such as the disruption of acid balance, the presence of digestive enzyme-inhibiting activators, and the difficulty in digestion and absorption of indigestible carbohydrates in plants. The amino acid balance can be overcome by using multiple feed families, but the development of new technologies has been desired for the decomposition and removal of digestive absorption inhibitors such as indigestible carbohydrates and phytin (phytic acid). Against this background, studies have been conducted to improve the digestibility of cultivated fish and shellfish feed by fermenting the koji mold Aspergillus sp.) On defatted soybean meal to enhance digestibility. Although it is inferior in growth performance, it has been reported that growth performance is improved as compared with the case of using unprocessed soybean meal (Non-Patent Document 1).
また、 特許文献 1では微生物を大豆粕に接種して発酵処理し、 大豆粕 中の蔗糖、 ラフイノース、 スタキオースなどの少糖類を分解除去する方 法が開示されている。 この際使用する微生物は Aspergillus氟、 >¾^^ 3 00? ^5属、 BacillusM, ac 。Z?aci JiAs属など通常の酸造食！?口、 発酵食品でされるものでよいとしており、 これらを接種して発酵処理し た大豆粕をハマチ稚魚に投与して成長性が改善されると開示している。 しかしながら、 植物性タンパク質原料が飼料原料中に占める割合が増 加するにつれて、 ハマチ、 マダイなど肉食性魚類は摂餌行動が低下する ことが知られており改善が必要とされており （非特許文献 2) 、 ォキア ミミールを 5〜 1 0 %添加して摂餌を誘引しなければならないのが実情 である。 Patent Document 1 discloses a method of inoculating soybean meal with a microorganism and performing fermentation treatment to decompose and remove oligosaccharides such as sucrose, raffinose, and stachyose in the soybean meal. The microorganism used in this case is Aspergillus 氟, > ¾ ^^ 300? ^ 5 genus, BacillusM, ac. Normal acid diet such as Z? Aci JiAs genus! It discloses that it can be made from fermented foods and fermented foods, and discloses that soybean meal that has been inoculated and fermented and administered to juvenile hamachi will improve growth. However, it is known that the feeding behavior of carnivorous fish such as yellowtail and red sea bream decreases as the proportion of the vegetable protein material in the feed material increases. 2) In fact, it is necessary to add 5 to 10% of Okia meal to attract food.
' また、 植物性タンパク質原料を動物飼料原料とする際には、 通常の醸 造や発酵食品で用いられる微生物を予備培養して植物性タンパク質原料 を発酵処理する必要があり、 工程の単純化が必要であった。 '' In addition, when using vegetable protein raw materials as animal feed raw materials, it is necessary to preculture microorganisms used in normal brewing and fermented foods and ferment the vegetable protein raw materials, which simplifies the process. Was needed.
特許文献 2にはクリル酵素 （ォキアミ酵素） を使用した水生動物用飼 料が開示されている。 これはォキアミ酵素の活性を保持したままで飼料 に混合するものである。 すなわち、 飼料とともにォキアミ酵素を給餌す ることにより動物の消化を助けるという考え方である。 ォキアミ酵素の 活性を保持したまま、 精製して飼料に添加しなければならないため、 加 熱することができないなど、 その取り扱いには制限が多く、 汎用性のあ る飼料原料としては使いにくいものである。  Patent Document 2 discloses a feed for aquatic animals using a krill enzyme (krill enzyme). In this method, the krill enzyme is mixed with feed while maintaining its activity. The idea is that feeding krill enzymes along with feed helps digestion of animals. Since it must be purified and added to the feed while maintaining the activity of the krill enzyme, it cannot be heated.Therefore, there are many restrictions on its handling, making it difficult to use as a versatile feed material. is there.
特許文献 3には自己消化させたォキアミを飼料原料として用いること が記載されている。 ォキアミに含まれるプロテアーゼにより消化されや すくなると記載されている。  Patent Literature 3 describes using autolyzed krill as a feed material. It is described that it is easily digested by a protease contained in krill.
特許文献 1 特開平 5— 2 6 8 8 8 1  Patent Literature 1 JP-A-5-26 8 8 8 1
特許文献 2 特開平 8— 24 2 7 7 7  Patent Document 2 Japanese Patent Application Laid-open No. Hei 8—24 2 7 7 7
特許文献 3 WO 9 8/344 9 8  Patent Document 3 WO 9 8/344 9 8
非特許文献 1 日本水産学会誌、 5 9卷、 1 8 8 3— 1 8 8 8 ( 1 9 9 3) Non-Patent Document 1 Journal of the Fisheries Society of Japan, Vol. 59, No. 1, 8 8 3— 1 8 8 8 (1 9 9 3)
非特許文献 2 Aoki, et al. ： Suisanzoshoku, 48, 73 - 79， (2000) 発明の開示  Non-Patent Document 2 Aoki, et al .: Suisanzoshoku, 48, 73-79, (2000) Disclosure of the Invention
本発明は、 大豆、 脱脂大豆粕、 小麦、 コーンダルテンミール、 米糠、 脱脂米糠、 加工糠、 ナタネ油粕、 綿実油粕、 ポテトプロテインなどの植 物性タンパク質を用いて、 該タンパク質の消化性を高め、 摂餌誘引効果 をもった動物用飼料原料、 ならびに、 それを原料とした動物用飼料を提 供することを目的とする。 本発明は、 以下の（1 )ないし（8)の動物用飼料原料を要旨とする。 The present invention uses planta proteins such as soybean, defatted soybean meal, wheat, corn dalten meal, rice bran, defatted rice bran, processed bran, oilseed rapeseed, cottonseed oilseed, and potato protein to enhance the digestibility of the protein, The purpose of the present invention is to provide an animal feed material having an effect of inducing feeding and an animal feed made from the same. The gist of the present invention is the following animal feed materials (1) to (8).
( 1 ) 植物性タンパク質原料の、 自己消化処理されていないォキアミ 成分による処理物からなる動物用飼料原料。 (1) An animal feed material comprising a vegetable protein material that has been treated with a krill component that has not been subjected to autolysis.
(2) 上記の処理物が、 植物性タンパク質原料と自己消化処理されて いないォキアミ成分を混合後、 熟成処理させて得られる処理物である上 記（1 )の動物用飼料原料。  (2) The animal feed material according to (1) above, wherein the processed material is obtained by mixing a vegetable protein raw material and a krill component that has not been subjected to autolysis, followed by aging treatment.
(3 ) ォキアミ成分が、 ォキアミ内在酵素または該内在酵素を含有す る酵素液である上記（ 1 )または（ 2 )の動物用飼料原料。  (3) The animal feed material according to (1) or (2), wherein the krill component is a krill endogenous enzyme or an enzyme solution containing the endogenous enzyme.
(4) 上記の酵素液が、 難消化性糖質を分解する酵素活性を有する酵 素液である上記（ 3 )の動物用飼料原料。  (4) The animal feed material according to the above (3), wherein the enzyme solution is an enzyme solution having an enzymatic activity for decomposing indigestible carbohydrates.
( 5 ) さらにプロテアーゼ阻害作用を有する飼料原料または飼料添 加物を併用することを特徴とする上記（1 )ないし（4)いずれかの動物用 飼料原料。  (5) The animal feed material according to any one of (1) to (4) above, further comprising a feed material or a feed additive having a protease inhibitory action.
(6 ) 植物性タンパク質原料が、 大豆、 脱脂大豆粕、 コーンダルテン ミール、 生糠、 加工糠、 小麦、 ナタネ油粕、 綿実油粕およびポテトプロ ティンからなる群より選ばれる 1種以上の植物性タンパク質原料である 上記（ 1 )ないし（ 5 )のいずれかの動物性飼料原料。 (6) The vegetable protein raw material is at least one vegetable protein raw material selected from the group consisting of soybean, defatted soybean meal, corn dalnut meal, raw bran, processed bran, wheat, rapeseed oil cake, cottonseed oil cake, and potato protein. The animal feed material according to any one of the above (1) to (5).
(7) ォキアミ成分が南極ォキアミ成分である上記（1)¾いし（6)い ずれかの動物用飼料原料。  (7) The animal feed material for animal feed according to any one of (1) and (6) above, wherein the krill component is an Antarctic krill component.
(8) 動物用飼料原料が魚類用、 棘皮類おょぴ Zまたは甲殻類用飼料 原料である上記（1 )ないし（7)のいずれかの動物用飼料原料。  (8) The animal feed material according to any of (1) to (7) above, wherein the animal feed material is fish, echinoderm Z or crustacean feed material.
(9) 魚類がタイ類、 サケ ·マス類、 プリ類、 マグロ類のいずれかに 属する魚である上記（8)の動物用飼料原料。  (9) The animal feed material of (8) above, wherein the fish is a fish belonging to any of Thailand, salmon and trout, puri, and tuna.
また、 本発明は、 以下の（1 0)の動物用飼料を要旨とする。  In addition, the gist of the present invention is the following animal feed (10).
(1 0) 上記（1)ないし（9)いずれかの動物用飼料原料を配合したこ とを特徴とする動物用飼料。 図面の簡単な説明  (10) An animal feed comprising the raw material for animal feed according to any one of the above (1) to (9). BRIEF DESCRIPTION OF THE FIGURES
第 1図は、 プロテアーゼ阻害作用を有するきな粉、 豆乳、 卵白のォキ アミ酵素に対する作用を示す図面である。 発明を実施するための最良の形態  FIG. 1 is a drawing showing the action of kinako, soymilk and egg white having protease inhibitory action on krill enzyme. BEST MODE FOR CARRYING OUT THE INVENTION
本発明における動物用飼料原料とは、 畜産、 養鶏、 養魚用に使用され る飼料原料であって、 タンパク質補給源として用いる飼料原料である。 本発明の飼料は特に養魚用、 甲殻類の養殖用に適している。 養魚、 棘皮 類、 甲殻類としてはマダイ、 サケ、 ハマチ、 シマアジ、 マス、 ヒラメ、 マグロ、 ゥナギ、 ゥニ、 ェビ等の養殖対象魚が例示される。 本発明において植物性タンパク質原料とは、 動物用飼料原料の動物性 タンパク質原料の代替品として用いられている植物性タンパク質原料で あれば何でも使用することができる。 主なものとしては、 大豆、 脱脂大 豆粕、 コーンダルテンミール、 生糠、 加工糠、 小麦、 ナタネ油粕、 綿実 油粕、 ポテトプロテイン等が例示される。 本発明において使用するォキアミは、 資源量が豊富で、 酵素活性が高 い南極ォキアミが好ましいが、 同様の活性のあるォキアミであれば他の ォキアミも使用できる。 The animal feed material in the present invention is a feed material used for livestock, poultry farming, and fish farming, and is a feed material used as a protein supplement source. The feed of the present invention is particularly suitable for fish farming and crustacean farming. Examples of fish culture, echinoderms and crustaceans include fish to be cultivated such as red sea bream, salmon, yellowtail, sardine, trout, flounder, tuna, penguin, squid and shrimp. In the present invention, any vegetable protein material can be used as long as it is a vegetable protein material used as a substitute for animal protein material in animal feed materials. The main ingredients are soybeans, defatted soybean meal, corn dalten meal, raw bran, processed bran, wheat, rapeseed oil cake, and cottonseed. Oil cake, potato protein and the like are exemplified. The krill used in the present invention is preferably Antarctic krill, which has abundant resources and high enzyme activity, but other krills having the same activity can be used.
ォキアミ成分としては、 ォキアミまたはミンチ処理したォキアミの 他に、 圧搾処理により選択的に得たォキアミ内在酵素を植物性タンパク 質原料に添加熟成することも可能である。 ただし、 これらォキアミ内在酵素を含有するォキアミ、 圧搾液は難消 化性糖質分解活性を有さなければならず、 その活性は薄層クロマトダラ フィ一にて容易に確認できる。 難消化性糖質の分解活性の測定は以下 に述べるようにして行う。 すなわち、 ォキアミであればォキアミをミン チ処理して固形分を分別除去し、 これに終濃度 1 %となるようにセロビ オースまたはマルトへキサオースなどの少糖類を加えて 4 0で、 2時間 攪拌処理を行う。 その後、 3000rPPm、 10分間、 4°Cにて遠心分離を行つ て得られる上清 10 Lをシリ力ゲル薄層プレート上にスポットし、 ブタ ノール： プロパノール： 7K ( 1 ： 3 ： 2 ) にて展開し、 オルシノール硫 酸溶液を用いて発色させる。 この際に、 セロビオース、 マルトへキサォ —スなどの少糖類のスポットが完全に消滅し、 β —グルコース、 a—ヴ ルコースなどと考えられる単糖のスポッ トのみが出現する活性を有する ものに限定される。 As the krill component, besides krill or minced krill, krill endogenous enzymes selectively obtained by squeezing can be added to the vegetable protein material and matured. However, the krill and compressed solution containing these krill endogenous enzymes must have an indegradable carbohydrate-decomposing activity, and the activity can be easily confirmed by thin-layer chromatography. The degradation activity of the indigestible saccharide is measured as described below. In other words, if the krill is minced, the krill is minced and the solid content is separated and removed.To this, oligosaccharides such as cellobiose or maltohexaose are added to a final concentration of 1%, and the mixture is stirred at 40 for 2 hours. Perform processing. Thereafter, 3000 r PP m, 10 minutes, spotted supernatant 10 L obtained Te Gyotsu centrifuged at 4 ° C in silica force gel thin layer plates, butanol: propanol: 7K (1: 3: 2 ) And color development using orcinol sulfuric acid solution. At this time, spots of oligosaccharides such as cellobiose and maltohexose are completely eliminated, and only those that have the activity of appearing spots of monosaccharides considered to be β-glucose, a-vulose, etc. Is done.
ォキアミ圧搾液に関しても同様の方法で活性を確認したものを用いる c 本発明の動物用飼料原料は、 難消化性糖質を含有する植物性タンパク 質原料にォキアミ、 ミンチ処理したォキアミ、 または圧搾液を加えて 2 0〜 6 0 °C、 好ましくは 3 0〜5 0 °Cにて 1〜4 8時間、 好ましくは 2 〜 6時間熟成処理を行い、 次いで 8 0 °C以上、 好ましくは 8 5 °C、 1 0 分間維持して加熱殺菌処理を行い、 乾燥処理に付することによって得ら れる。 熟成の際に、 撹拌処理を行うが、 撹拌が困難で熟成が不充分とな らないように清水を加えることも可能である。 反応時間は植物性タンパ ク質に対する酵素量によって調節する必要があるが、 遊離アミノ酸が多 くなると飼料効率が悪くなるので、 遊離ァミノ酸が生成しにくい条件を 選択するのが好ましい。 本発明の動物用飼料原料を原料として使用した動物用飼料。 Animal feed ingredients of c present invention to use one confirming the activity in a similar manner with respect Okiami pressate is Okiami vegetable protein material containing indigestible saccharides, Okiami minced treated or pressate, Plus 2 Aging treatment is carried out at 0 to 60 ° C, preferably 30 to 50 ° C for 1 to 48 hours, preferably 2 to 6 hours, and then at 80 ° C or more, preferably 85 ° C, 1 It is obtained by performing heat sterilization while maintaining for 0 minutes and then subjecting it to drying. During ripening, stirring is performed, but it is also possible to add fresh water so that stirring is difficult and ripening is not insufficient. The reaction time needs to be adjusted depending on the amount of the enzyme relative to the vegetable protein. However, if the amount of free amino acids increases, the feed efficiency deteriorates. Animal feed using the animal feed material of the present invention as a raw material.
本発明の動物用飼料は本発明の動物用飼料原料及びその他の飼料原 料を一緒に加工成形して製造することができる。 本発明の動物用飼料原 料はォキアミ処理後、 加熱乾燥して粉末状の形態で提供できるもので、 種々のタイプの飼料用原料として、 特に動物性タンパク質の代替品とし て容易に使用することができる。 養魚用の場合でいえば、 魚粉の代替品 として使用することができる。 作用  The animal feed of the present invention can be produced by processing and shaping the animal feed raw material of the present invention and other feed raw materials together. The animal feed raw material of the present invention can be provided in powder form by heating and drying after krill treatment, and can be easily used as a raw material for various types of feed, particularly as a substitute for animal protein. Can be. In the case of fish farming, it can be used as a substitute for fish meal. Action
難消化性糖質分解活性を有するォキアミ、 ミンチ処理したォキアミあ るいはォキアミ圧搾液を難消化性糖質含有の植物性タンパク質原料に添 加し熟成することにより、 タンパク質の消化性が向上する。  Protein digestibility is improved by adding krill having indigestible carbohydrate-decomposing activity, minced krill or krill squeezed solution to the indigestible carbohydrate-containing plant protein raw material and ripening it.
また、 該タンパク質の消化性を高め、 摂餌誘引効果をもった動物用飼料 原料とする。 In addition, it is used as an animal feed raw material that has an enhanced digestibility of the protein and has a feed-inducing effect.
動物タンパク質原料の使用割合を著しく低減できる動物用飼料原料を 提供することが可能となった。 また、 その動物性飼料原料を使用した動 物用飼料を提供することができる。 本願発明の詳細を実施例で説明する。 本願発明はこれらの実施例によ つて何ら限定されるものではない。 It has become possible to provide an animal feed material that can significantly reduce the proportion of animal protein material used. In addition, it is possible to provide animal feed using the animal feed material. Examples of the present invention will be described in detail. The present invention is not limited in any way by these examples.
実施例 1  Example 1
難消化性糖質分解活性を有するォキアミ 2 5 k gをミンチ処理した後、 脱脂大豆粕 3 O kgを加えて 4 0 °C、 2時間加熱攪拌処理して分解脱脂大 豆粕を作製した （以下、 ォキアミミンチ処理大豆粕と称する） 。  After mincing 25 kg of krill having indigestible carbohydrate-decomposing activity, 3 O kg of defatted soybean meal was added, and the mixture was heated and stirred at 40 ° C for 2 hours to produce degraded defatted soybean meal (hereinafter, referred to as “ Komi-minced soybean meal).
実施例 2  Example 2
難消化性糖質分解活性を有するォキアミを圧搾処理して、 ォキアミ原 料の 1 0 %重量の圧搾液 （酵素液） を作製し、 これに脱脂大豆粕 3 O kg を加えて 4 0 °C、 2時間加熱攪拌処理して分解脱脂大豆粕を作製した (以下、 ォキアミ酵素液処理大豆粕と称する） 。  Krill having indigestible carbohydrate-decomposing activity is squeezed to prepare a squeezed liquid (enzyme solution) having a weight of 10% of krill raw material, and 3 O kg of defatted soybean meal is added to the mixture at 40 ° C. Then, the mixture was heated and stirred for 2 hours to prepare decomposed defatted soybean meal (hereinafter referred to as krill enzyme solution-treated soybean meal).
実施例 3  Example 3
難消化性糖質分解活性を有するォキアミ 2 5 k gをミンチ処理した後、 卵白 2 5 0 g、 脱脂大豆粕 3 O kgを加えて 4 0 、 2時間加熱攪拌処理 して分解脱脂大豆粕を作製した （以下、 ォキアミ処理大豆粕卵白添加と 称する） 。  After mincing 25 kg of krill, which has indigestible carbohydrate-decomposing activity, add 250 g of egg white and 3 O kg of defatted soybean meal, and heat and stir for 40 or 2 hours to produce degraded defatted soybean meal. (Hereinafter referred to as krill-treated soybean meal egg white addition).
実施例 4  Example 4
マダイ （平均体重 17. 9g) を試験魚に用いて 5 6 日間の飼育実験を行 つた。 用いた飼育用飼料の配合組成を表 1に、 飼育結果を表 2、 3に示 した。 マダイ飼育試験用飼料の配合組成 （％) Red sea bream (average body weight: 17.9 g) was used as a test fish for a breeding experiment for 56 days. Table 1 shows the composition of the breeding feed used, and Tables 2 and 3 show the breeding results. Composition of feed for red sea bream test (%)
Figure imgf000011_0001
表 2
Figure imgf000011_0001
Table 2
1区 2区 3区 4区 実施例 1 実施例 3 実施例 2 開始時 17. 9 17. 9 17. 8 17. 8 飼育 8週目 60. 6 59. 5 61. 1 62. 0 体重増加（ g ) 42. 7 41. 6 43. 2 44. 2 表 3 1st section 2nd section 3rd section 4th section Example 1 Example 3 Example 2 At start 17.9 17.9 17.8 17.8 Rearing Week 8 60. 6 59. 5 61. 1 62.0 Weight gain ( g) 42. 7 41. 6 43. 2 44.2 Table 3
Figure imgf000012_0001
この結果から明らかなように、 脱脂大豆粕を無添加の 1区と成長には 差が認められず、 マダイ用飼料にォキアミのミンチや圧搾液 （酵素液） を用いて処理した脱脂大豆粕を多量添加できることが分かり、 ォキアミ による熟成処理の有効性が確認された。
Figure imgf000012_0001
As is evident from the results, there was no difference in the growth from that in the 1st plot where no defatted soybean meal was added. It was found that a large amount could be added, and the effectiveness of the ripening treatment with krill was confirmed.
プロテアーゼ阻害作用を有する卵白を添加しても （3区） 、 卵白を添 加しない区 （2区） と比較して遜色のない結果が得られた。 また、 飼料 中の遊離ァミノ酸ノ総ァミノ酸（％)と飼料効率には負の相関が認められ れた。 実施例 5  Even when egg white having a protease inhibitory action was added (section 3), the results were comparable to those without egg white (section 2). In addition, a negative correlation was found between the free amino acids in the feed and the total amino acids (%) and feed efficiency. Example 5
-ジマス （平均体重 10. 6g) を試験魚に用いて 84日間の飼育実験を行 つた。 用いた飼育用飼料の配合組成を表 4に、 飼育結果を表 5 、 6に示 した。 表 4 -84 days of breeding experiments were carried out using jimas (average body weight: 10.6 g) as test fish. Table 4 shows the composition of the feed used for breeding, and Tables 5 and 6 show the breeding results. Table 4
Figure imgf000013_0001
表 5
Figure imgf000013_0001
Table 5
ニジマスの飼育試験結果  Rearing test result of rainbow trout
5区 6区 8区 9区 10区 魚粉のみ 実施例 1 実施例 2 ォキアミミール 市販酵素処理 開始時 10.63 10.61 10.59 10.61 10.60 終了時（84曰） 27.24 27.61 29.15 25.06 23.61 体重増加（g) 16.61 17.00 18.56 14.45 13.01 表 6 5th section 6th section 8th section 9th section 10th section Fish meal only Table 6
Figure imgf000014_0001
表 5から明らかなように、 脱脂大豆粕を添加しなかった対照区に比し て、 ォキアミミンチ処理区 （実施例 1 ) 、 ォキアミ酵素液処理区 （実施 例 3 ) は体重増加 （成長性） の点で有効であった。 また、 ォキアミミー ルのみを混合した 9区よりも効果があり、 ォキアミで脱脂大豆粕を処理 することが有効であることが確認された。 更に、 通常市販されているセ ルラーゼ処理 （酵素処理区） では、 成長が劣ることも分かり、 ォキアミ による熟成処理の必要性が分かった。
Figure imgf000014_0001
As is clear from Table 5, the kaki mince-treated group (Example 1) and the krill enzyme solution-treated group (Example 3) showed lower weight gain (growth) than the control group to which no defatted soybean meal was added. It was effective in terms. In addition, it was more effective than the 9th section where only krill was mixed, and it was confirmed that treating defatted soybean meal with krill was effective. Furthermore, it was also found that the growth was inferior in the case of cellulase treatment (enzyme-treated section) which is usually commercially available, indicating the necessity of ripening treatment with krill.
また、 飼料中の遊離ァミノ酸/総ァミノ酸（wと飼料効率には負の相 関が認められれた。 試験例 1 ： ォキアミ圧搾液の酵素活性の阻害剤による影響  In addition, a negative correlation was found between the free amino acid / total amino acid in the feed (w and feed efficiency. Test Example 1: Influence of inhibitors of the enzyme activity of krill squeezed juice)
本試験例では、 2 0 0 2年 2月に漁獲したォキアミより調製した圧搾 液を 「夏」 ロット、 2 0 0 2年 5月に漁獲したォキアミより調製した圧 搾液を 「冬」 ロッ トと記載する。 これは漁獲地である南極海における季 節を反映させた命名である。  In this test example, the squeezed liquid prepared from krill caught in February 2002 was a `` summer '' lot, and the squeezed liquid prepared from krill caught in May 2002 was a `` winter '' lot It is described. This is a name that reflects the seasons in the Antarctic Ocean where the fish are harvested.
きな粉 （焙煎大豆粉） および卵白粉末のそれぞれ 1 2 % ( w / v ) 濃 度の懸濁液を調製した。 夏おょぴ冬ロットのォキアミ圧搾液 3 . O m L に対して、 蒸留水、 きな粉懸濁液、 豆乳（固形分 12%w/v)、 または卵白懸 濁液を 0 . I m L添加した。 このとき、 きな粉、 豆乳、 または卵白は固 形分換算で 0 . 4 %濃度と計算される。 これら添加したォキアミ圧搾液 のプロテアーゼ活性、 および糖質分解酵素活性を測定した。 蒸留水 （即 ち固形分なし） を添加した場合の活性 （吸光度の上昇値） を 1 0 0 %と して、 各添加物を添加した場合の活性を計算した。 Kinase powder (roasted soybean powder) and egg white powder were each prepared at a concentration of 12% (w / v). To 3.000 mL of krill squeezed juice of summer / winter lot, distilled water, kinako suspension, soy milk (solid content 12% w / v), or egg white suspension The suspension was added at 0.1 mL. At this time, the content of kinako, soymilk, or egg white is calculated to be 0.4% in solid content. The protease activity and carbohydrate-degrading enzyme activity of the krill squeezed solution added were measured. The activity (increase in absorbance) when distilled water (immediately without solids) was added was set to 100%, and the activity when each additive was added was calculated.
第 1図に示すように、 きな粉、 豆乳、 および、 卵白はいずれのロッ ト についてもォキアミ圧搾液のプロテアーゼ活性を阻害する一方、 糖質分 解酵素活性には影響を与えないことがわかった。 産業上の利用可能性  As shown in FIG. 1, it was found that kinako, soymilk, and egg white inhibited the protease activity of the krill squeezed juice but did not affect the carbohydrate degrading enzyme activity in any of the lots. Industrial applicability
本発明は、 植物性タンパク質を用いて、 該タンパク質の消化性が向上 し、 かつ、 摂餌誘引効果をもった動物用飼料原料を提供することができ る。 .  INDUSTRIAL APPLICABILITY The present invention can provide an animal feed raw material using a vegetable protein, in which digestibility of the protein is improved and which has an effect of inducing food intake. .
また、 本発明は、 その動物用飼料原料を原料と しとした動物タンパ ク質原料の使用割合を著しく低減できる動物用飼料を提供することがで きる。  Further, the present invention can provide an animal feed that can significantly reduce the use ratio of animal protein raw materials using the animal feed raw materials as raw materials.

Claims

請 求 の 範 囲 The scope of the claims
1 . 植物性タンパク質原料の、 自已消化処理されていないォキアミ成 分による処理物からなる動物用飼料原料。 1. An animal feed material consisting of a vegetable protein material that has been processed with krill components that have not been digested.
2 . 上記の処理物が、 植物性タンパク質原料と自己消化処理されてい ないォキアミ成分を混合後、 熟成処理させて得られる処理物である請求 項 1の動物用飼料原料。 2. The animal feed material according to claim 1, wherein the processed material is a processed product obtained by mixing a vegetable protein material and a krill component that has not been subjected to autolysis, followed by aging treatment.
3 . ォキアミ成分が、 ォキアミ内在酵素または該内在酵素を含有する 酵素液である請求項 1または 2の動物用飼料原料。  3. The animal feed material according to claim 1 or 2, wherein the krill component is a krill endogenous enzyme or an enzyme solution containing the endogenous enzyme.
4 . 上記の酵素液が、 難消化性糖質を分解する酵素活性を有する酵素 液である請求項 3の動物用飼料原料。 4. The animal feed material according to claim 3, wherein said enzyme solution is an enzyme solution having an enzymatic activity for decomposing indigestible carbohydrates.
5 . さらにプロテアーゼ阻害作用を有する飼料原料または飼料添加物 を併用することを特徴とする請求項 1ないし 4いずれかの動物用飼料原 料。  5. The animal feed material according to any one of claims 1 to 4, further comprising a feed material or a feed additive having a protease inhibitory action.
6 . 植物性タンパク質原料が、 大豆、 脱脂大豆粕、 コーンダルテンミ ール、 生糠、 加工糠、 小麦、 ナタネ油粕、 綿実油粕およびポテトプロテ ィンからなる群より選ばれる 1種以上の植物性タンパク質原料である請 求項 1ないし 5のいずれかの動物性飼料原料。 6. One or more vegetable proteins selected from the group consisting of soybeans, defatted soybean meal, corn dalnut meal, raw bran, processed bran, wheat, rapeseed oil cake, cottonseed oil cake, and potato protein. The animal feed material according to any one of claims 1 to 5, which is a raw material.
7 . ォキアミ成分が南極ォキアミ成分である請求項 1ないし 6のいず れかの動物用飼料原料。  7. The animal feed material according to any one of claims 1 to 6, wherein the krill component is an Antarctic krill component.
8 . 動物用飼料原料が魚類用、 棘皮類および または甲殻類用飼料原 料である請求項 1ないし 7のいずれかの動物用飼料原料。  8. The animal feed material according to any one of claims 1 to 7, wherein the animal feed material is a feed material for fish, echinoderms and / or crustaceans.
9 . 魚類がタイ類、 サケ ·マス類、 プリ類、 マグロ類である請求項 8 の動物用飼料原料。  9. The animal feed material according to claim 8, wherein the fishes are Thai, salmon and trout, puri, and tuna.
1 0 . 請求項 1ないし 9のいずれかの動物用飼科原料を配合したこと を特徴とする動物用飼料。  10. An animal feed comprising the animal feed material according to any one of claims 1 to 9.
PCT/JP2003/009773 2002-08-09 2003-08-01 Animal feed material and animal feed using the material WO2004014145A1 (en)

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AU2003252452A AU2003252452B2 (en) 2002-08-09 2003-08-01 Animal feed material and animal feed using the material
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102763766A (en) * 2012-04-27 2012-11-07 田卫东 Biological feed containing pomace and bean dregs and preparation method thereof
CN102845645A (en) * 2012-09-20 2013-01-02 合肥基石生物工程有限公司 Preparation method of soluble easily-digested feed for young livestock and poultry
CN104054938A (en) * 2014-05-27 2014-09-24 范治淮 Health coated grass carp feed and preparation method thereof
CN104054936A (en) * 2014-05-27 2014-09-24 范治淮 High-efficiency feed special for grass carp and preparation method of high-efficiency feed
RU2537547C1 (en) * 2013-07-02 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Дальневосточный государственный технический рыбохозяйственный университет" Trepang fodder product preparation method
RU2555826C2 (en) * 2013-05-07 2015-07-10 Федеральное государственное бюджетное научное учреждение "Тихоокеанский научно-исследовательский рыбохозяйственный центр" (ФГБНУ "ТИНРО-Центр") Method of obtaining production combined feed for young sea cucumber and its implementation

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0729708A1 (en) * 1995-03-03 1996-09-04 F. Hoffmann-La Roche Ag Use of krill enzymes in animal feedstuffs
WO1999039589A1 (en) * 1998-02-09 1999-08-12 Biozyme Systems Inc. Method and apparatus for processing krill hydrolysates

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2197137A1 (en) * 1997-02-07 1998-08-07 Biozyme Systems Inc. Method and apparatus for co-drying krill hydrolysate, liquid marine protein and dry carrier

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0729708A1 (en) * 1995-03-03 1996-09-04 F. Hoffmann-La Roche Ag Use of krill enzymes in animal feedstuffs
WO1999039589A1 (en) * 1998-02-09 1999-08-12 Biozyme Systems Inc. Method and apparatus for processing krill hydrolysates

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102763766A (en) * 2012-04-27 2012-11-07 田卫东 Biological feed containing pomace and bean dregs and preparation method thereof
CN102845645A (en) * 2012-09-20 2013-01-02 合肥基石生物工程有限公司 Preparation method of soluble easily-digested feed for young livestock and poultry
RU2555826C2 (en) * 2013-05-07 2015-07-10 Федеральное государственное бюджетное научное учреждение "Тихоокеанский научно-исследовательский рыбохозяйственный центр" (ФГБНУ "ТИНРО-Центр") Method of obtaining production combined feed for young sea cucumber and its implementation
RU2537547C1 (en) * 2013-07-02 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Дальневосточный государственный технический рыбохозяйственный университет" Trepang fodder product preparation method
CN104054938A (en) * 2014-05-27 2014-09-24 范治淮 Health coated grass carp feed and preparation method thereof
CN104054936A (en) * 2014-05-27 2014-09-24 范治淮 High-efficiency feed special for grass carp and preparation method of high-efficiency feed
CN104054938B (en) * 2014-05-27 2016-05-18 范治淮 A kind of health care coating feed of grass carp and preparation method thereof

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AU2003252452A1 (en) 2004-02-25
JPWO2004014145A1 (en) 2005-11-24
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AU2003252452B2 (en) 2008-02-21
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CA2494945C (en) 2011-03-22

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