WO2003066667A3 - Method and apparatus for performing submicroliter reactions with nucleic acids or proteins - Google Patents

Method and apparatus for performing submicroliter reactions with nucleic acids or proteins Download PDF

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Publication number
WO2003066667A3
WO2003066667A3 PCT/US2003/003986 US0303986W WO03066667A3 WO 2003066667 A3 WO2003066667 A3 WO 2003066667A3 US 0303986 W US0303986 W US 0303986W WO 03066667 A3 WO03066667 A3 WO 03066667A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
nucleic acids
capillary
reactions
methods
Prior art date
Application number
PCT/US2003/003986
Other languages
French (fr)
Other versions
WO2003066667A2 (en
Inventor
Stevan Bogdan Jovanovich
Oscar Salas-Solano
Jeng-Thun Li
Original Assignee
Amersham Biosciences Sv Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/262,476 external-priority patent/US6927045B2/en
Application filed by Amersham Biosciences Sv Corp filed Critical Amersham Biosciences Sv Corp
Priority to CA002474429A priority Critical patent/CA2474429A1/en
Priority to AU2003217369A priority patent/AU2003217369A1/en
Priority to JP2003566038A priority patent/JP2005525797A/en
Priority to EP03713412A priority patent/EP1567656A2/en
Priority to CN03807801.5A priority patent/CN1791680A/en
Publication of WO2003066667A2 publication Critical patent/WO2003066667A2/en
Publication of WO2003066667A3 publication Critical patent/WO2003066667A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B01L9/065—Test-tube stands; Test-tube holders specially adapted for capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B01J2219/00369—Pipettes capillary in multiple or parallel arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00691—Automatic using robots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for preparing nanoscale reactions using nucleic acids or proteins are presented, Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Proteins are captured specifically and saturably on the modified inner surface of the reaction chamber, typically a capillary. Excess protein is removed and the reaction is performed directly within the capillary. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput reactions involving nucleic acids or proteins are also provided.
PCT/US2003/003986 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reactions with nucleic acids or proteins WO2003066667A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002474429A CA2474429A1 (en) 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reactions with nucleic acids or proteins
AU2003217369A AU2003217369A1 (en) 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reactions with nucleic acids or proteins
JP2003566038A JP2005525797A (en) 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reaction using nucleic acid or protein
EP03713412A EP1567656A2 (en) 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reactions with nucleic acids or proteins
CN03807801.5A CN1791680A (en) 2003-02-07 2003-02-07 Method and device for performing submicroliter reactions with nucleic acids or proteins

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US35564802P 2002-02-08 2002-02-08
US35566002P 2002-02-08 2002-02-08
US60/355,660 2002-02-08
US60/355,648 2002-02-08
US10/262,476 2002-09-30
US10/262,476 US6927045B2 (en) 1999-08-02 2002-09-30 Methods and apparatus for template capture and normalization for submicroliter reaction

Publications (2)

Publication Number Publication Date
WO2003066667A2 WO2003066667A2 (en) 2003-08-14
WO2003066667A3 true WO2003066667A3 (en) 2005-06-02

Family

ID=27739086

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/003986 WO2003066667A2 (en) 2002-02-08 2003-02-07 Method and apparatus for performing submicroliter reactions with nucleic acids or proteins

Country Status (5)

Country Link
EP (1) EP1567656A2 (en)
JP (1) JP2005525797A (en)
AU (1) AU2003217369A1 (en)
CA (1) CA2474429A1 (en)
WO (1) WO2003066667A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050282270A1 (en) * 2004-06-21 2005-12-22 Applera Corporation System for thermally cycling biological samples with heated lid and pneumatic actuator
CA2564310A1 (en) * 2005-11-07 2007-05-07 Nisshinbo Industries, Inc. Tip for biomolecular reaction
KR20110101143A (en) * 2008-11-04 2011-09-15 블러드 셀 스토리지 인코퍼레이티드 Nucleic acid extraction on curved glass surfaces
EP2848309B1 (en) * 2013-09-13 2020-06-10 NanoTemper Technologies GmbH Holder for capillaries
US9993819B2 (en) 2014-12-30 2018-06-12 Stmicroelectronics S.R.L. Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus
GB201501603D0 (en) * 2015-01-30 2015-03-18 Norwegian University Of Life Sciences And Hedmark University College Method and product
CN112304699A (en) * 2019-08-01 2021-02-02 利多(香港)有限公司 Reagent storage device and application in detection kit
CN114441264B (en) * 2022-01-20 2023-05-30 复旦大学 Skin upgrading volume single-cell sample schizolysis enzymolysis reactor

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846727A (en) * 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US6290995B1 (en) * 2000-02-08 2001-09-18 Zhao Xinxian Plant drug for preventing cancer II
US6489112B1 (en) * 1999-08-02 2002-12-03 Molecular Dynamics, Inc. Methods and apparatus for template capture and normalization for submicroliter reaction

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846727A (en) * 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US6489112B1 (en) * 1999-08-02 2002-12-03 Molecular Dynamics, Inc. Methods and apparatus for template capture and normalization for submicroliter reaction
US6290995B1 (en) * 2000-02-08 2001-09-18 Zhao Xinxian Plant drug for preventing cancer II

Also Published As

Publication number Publication date
AU2003217369A1 (en) 2003-09-02
EP1567656A2 (en) 2005-08-31
WO2003066667A2 (en) 2003-08-14
JP2005525797A (en) 2005-09-02
AU2003217369A8 (en) 2003-09-02
CA2474429A1 (en) 2003-08-14

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