WO2003039459A3 - Viral vector production methods and compositions - Google Patents

Viral vector production methods and compositions Download PDF

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Publication number
WO2003039459A3
WO2003039459A3 PCT/US2002/035049 US0235049W WO03039459A3 WO 2003039459 A3 WO2003039459 A3 WO 2003039459A3 US 0235049 W US0235049 W US 0235049W WO 03039459 A3 WO03039459 A3 WO 03039459A3
Authority
WO
WIPO (PCT)
Prior art keywords
viral vector
compositions
production methods
vector production
provides methods
Prior art date
Application number
PCT/US2002/035049
Other languages
French (fr)
Other versions
WO2003039459A2 (en
Inventor
Lee-Cheng Liu
Perry Newton
Shoupeng Lai
Stephen Morris
Chad Atwell
Christon Hill
Megan Fitzpatrick
Sami Cardak
Alena Lizonova
Lu Qin
Miguel E Carrion
Brenk K Harris
Original Assignee
Genvec Inc
Lee-Cheng Liu
Perry Newton
Shoupeng Lai
Stephen Morris
Chad Atwell
Christon Hill
Megan Fitzpatrick
Sami Cardak
Alena Lizonova
Lu Qin
Miguel E Carrion
Brenk K Harris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genvec Inc, Lee-Cheng Liu, Perry Newton, Shoupeng Lai, Stephen Morris, Chad Atwell, Christon Hill, Megan Fitzpatrick, Sami Cardak, Alena Lizonova, Lu Qin, Miguel E Carrion, Brenk K Harris filed Critical Genvec Inc
Priority to AU2002348151A priority Critical patent/AU2002348151A1/en
Publication of WO2003039459A2 publication Critical patent/WO2003039459A2/en
Publication of WO2003039459A3 publication Critical patent/WO2003039459A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods of preparing viral vector particles and viral vector particle compositions.
PCT/US2002/035049 2001-11-05 2002-10-31 Viral vector production methods and compositions WO2003039459A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002348151A AU2002348151A1 (en) 2001-11-05 2002-10-31 Viral vector production methods and compositions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33287301P 2001-11-05 2001-11-05
US60/332,873 2001-11-05
US33212001P 2001-11-13 2001-11-13
US60/332,120 2001-11-13

Publications (2)

Publication Number Publication Date
WO2003039459A2 WO2003039459A2 (en) 2003-05-15
WO2003039459A3 true WO2003039459A3 (en) 2003-11-20

Family

ID=26988068

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035049 WO2003039459A2 (en) 2001-11-05 2002-10-31 Viral vector production methods and compositions

Country Status (2)

Country Link
AU (1) AU2002348151A1 (en)
WO (1) WO2003039459A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12006510B2 (en) 2021-06-25 2024-06-11 Genzyme Corporation Perfusion culturing methods and uses thereof

Families Citing this family (26)

* Cited by examiner, † Cited by third party
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WO2007016250A1 (en) * 2005-07-28 2007-02-08 Genvec, Inc. Method of purifying protein
RU2603740C2 (en) 2009-05-02 2016-11-27 Джензим Корпорейшн Gene therapy for neurodegenerative disorders
SG10201500015TA (en) * 2010-01-12 2015-02-27 Vascular Biogenics Ltd Methods of producing adenovirus vectors and viral preparations generated thereby
WO2013076309A1 (en) * 2011-11-24 2013-05-30 Genethon Scalable lentiviral vector production system compatible with industrial pharmaceutical applications
KR102015933B1 (en) * 2012-04-08 2019-08-29 인벤트프라이즈 엘엘씨 Systems and methods of virus propagation in cell culture for vaccine manufacture
ES2717927T3 (en) * 2013-02-22 2019-06-26 Genzyme Corp Cultivation procedures by perfusion of microcarriers and uses thereof
MX367025B (en) * 2013-02-22 2019-08-02 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof.
AU2014232879B2 (en) * 2013-03-15 2020-03-05 The Children's Hospital Of Philadelphia Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system
CA2920191C (en) 2013-07-18 2022-03-01 Children's Hospital Medical Center Methods of improving titer in transfection-based production systems using eukaryotic cells
EP3613841B1 (en) 2014-03-25 2022-04-20 Terumo BCT, Inc. Passive replacement of media
TWI743024B (en) 2014-06-06 2021-10-21 美商健臻公司 Perfusion culturing methods and uses thereof
TWI776235B (en) 2014-06-09 2022-09-01 美商健臻公司 Seed train processes and uses thereof
BR112017013423B1 (en) 2014-12-22 2023-04-11 Genzyme Corporation MAMMALIAN CELL CULTIVATION METHODS
EP3054007A1 (en) 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
CA3040288A1 (en) * 2016-11-04 2018-07-12 Baxalta Incorporated Adeno-associated virus purification methods
US11702634B2 (en) 2017-03-31 2023-07-18 Terumo Bct, Inc. Expanding cells in a bioreactor
KR102643016B1 (en) * 2017-04-10 2024-02-29 에피센트알엑스, 인코포레이티드 Method for producing recombinant virus
US11603527B2 (en) * 2017-12-27 2023-03-14 Global Life Sciences Solutions Usa Llc Method and kit for viral vector isolation
CN110317832B (en) * 2018-03-28 2022-07-05 西比曼生物科技（香港）有限公司 GMP (good manufacturing practice) scale preparation method of purified preparation of recombinant lentiviral vector
CN110317791A (en) 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 The method of GMP grades of serum free suspension cell large-scale production slow virus
CN114181970B (en) * 2020-09-15 2023-07-25 上海药明巨诺生物医药研发有限公司 Lentiviral vector purification method
CN116829180A (en) * 2020-10-23 2023-09-29 弹性政府服务公司 Methods for infecting cells with viruses

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143548A (en) * 1995-08-30 2000-11-07 Genzyme Corporation Chromatographic purification of adeno-associated virus (AAV)
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks
US6194191B1 (en) * 1996-11-20 2001-02-27 Introgen Therapeutics, Inc. Method for the production and purification of adenoviral vectors
US6303362B1 (en) * 1998-11-19 2001-10-16 The Board Of Trustees Of The Leland Stanford Junior University Adenoviral vector and methods for making and using the same
US20010036657A1 (en) * 1996-12-13 2001-11-01 Tang John Chu-Tay Methods for purifying viruses

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143548A (en) * 1995-08-30 2000-11-07 Genzyme Corporation Chromatographic purification of adeno-associated virus (AAV)
US6194191B1 (en) * 1996-11-20 2001-02-27 Introgen Therapeutics, Inc. Method for the production and purification of adenoviral vectors
US20010036657A1 (en) * 1996-12-13 2001-11-01 Tang John Chu-Tay Methods for purifying viruses
US6303362B1 (en) * 1998-11-19 2001-10-16 The Board Of Trustees Of The Leland Stanford Junior University Adenoviral vector and methods for making and using the same
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12006510B2 (en) 2021-06-25 2024-06-11 Genzyme Corporation Perfusion culturing methods and uses thereof

Also Published As

Publication number Publication date
AU2002348151A1 (en) 2003-05-19
WO2003039459A2 (en) 2003-05-15

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