WO2001079546A3 - Detection de flavivirus et analyse de quantification - Google Patents

Detection de flavivirus et analyse de quantification Download PDF

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Publication number
WO2001079546A3
WO2001079546A3 PCT/US2000/028961 US0028961W WO0179546A3 WO 2001079546 A3 WO2001079546 A3 WO 2001079546A3 US 0028961 W US0028961 W US 0028961W WO 0179546 A3 WO0179546 A3 WO 0179546A3
Authority
WO
WIPO (PCT)
Prior art keywords
flavivirus
fluorogenic
disclosed
permit
dengue virus
Prior art date
Application number
PCT/US2000/028961
Other languages
English (en)
Other versions
WO2001079546A2 (fr
Inventor
Huo-Shu H Houng
Niranjan Kanesa-Thasan
Original Assignee
Us Army Med Res Mat Command
Huo-Shu H Houng
Niranjan Kanesa-Thasan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/551,161 external-priority patent/US6793488B1/en
Application filed by Us Army Med Res Mat Command, Huo-Shu H Houng, Niranjan Kanesa-Thasan filed Critical Us Army Med Res Mat Command
Priority to AU2001212172A priority Critical patent/AU2001212172A1/en
Priority to BR0017215-4A priority patent/BR0017215A/pt
Priority to CA002405960A priority patent/CA2405960A1/fr
Priority to EP00973687A priority patent/EP1276898A1/fr
Publication of WO2001079546A2 publication Critical patent/WO2001079546A2/fr
Publication of WO2001079546A3 publication Critical patent/WO2001079546A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des sondes d'ADN spécifiques fluorescentes et des paires d'amorces flanquantes sont conçues sur la base des informations de séquence trouvées dans la région conservée terminale 3' non codante de flavivirus, par ex. les nucléotides 10653-10678 du virus de la dengue. L'amplification en chaîne par polymérase (PCR) fluorogène employant ces amorces et sondes produit des résultats qui permettent l'identification spécifique de flavivirus. Les analyses peuvent être tant qualitatives que quantitatives. Des conditions optimales d'analyse avec bruit de fond nul qui permettent la détection de faibles niveaux de flavivirus dans des spécimens cliniques sont également décrites. Spécifiquement, des virus de dengue isolés dans différentes régions géographiques peuvent être universellement détectés et identifiés par l'analyse fondée sur la RT-PCR fluorogène décrite. Ladite analyse RT-PCR fluorogène a permis de détecter facilement une virémie dans des sérums prélevés chez des patients atteints de la dengue.
PCT/US2000/028961 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification WO2001079546A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001212172A AU2001212172A1 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay
BR0017215-4A BR0017215A (pt) 2000-04-14 2000-10-19 Detecção de flavivìrus e análises de quantificação
CA002405960A CA2405960A1 (fr) 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification
EP00973687A EP1276898A1 (fr) 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/551,161 2000-04-14
US09/551,161 US6793488B1 (en) 1999-04-16 2000-04-14 Flavivirus detection and quantification assay

Publications (2)

Publication Number Publication Date
WO2001079546A2 WO2001079546A2 (fr) 2001-10-25
WO2001079546A3 true WO2001079546A3 (fr) 2003-06-05

Family

ID=24200105

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028961 WO2001079546A2 (fr) 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification

Country Status (5)

Country Link
EP (1) EP1276898A1 (fr)
AU (1) AU2001212172A1 (fr)
BR (1) BR0017215A (fr)
CA (1) CA2405960A1 (fr)
WO (1) WO2001079546A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2890882T3 (es) * 2002-10-16 2022-01-24 Gen Probe Inc Composiciones y procedimientos para detectar el virus del Nilo occidental
AU2008200221B2 (en) * 2002-10-16 2012-04-05 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
WO2013080307A1 (fr) * 2011-11-29 2013-06-06 株式会社 東芝 Jeu d'amorces pour amplifier les virus transmis par les moustiques, kit de dosage pour détecter les virus transmis par les moustiques, et procédé de détection faisant appel audit jeu d'amorces et audit kit de dosage
CN104342498B (zh) * 2013-07-30 2019-04-16 中国疾病预防控制中心病毒病预防控制所 库蚊黄病毒实时荧光定量rt-pcr检测方法及试剂盒
CN104357582B (zh) * 2014-10-31 2017-02-15 中国检验检疫科学研究院 库京病毒的实时荧光rt‑pcr检测试剂盒和方法
CN107893128A (zh) * 2017-11-09 2018-04-10 贵州医科大学 同时检测10种虫媒病毒的引物对组合、探针及应用
CN110846441B (zh) * 2019-12-20 2023-04-11 浙江省淡水水产研究所 检测沼虾黄病毒-1的特异性引物、探针及快速检测试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999034020A1 (fr) * 1997-12-31 1999-07-08 Akzo Nobel N.V. Dosage base sur la transcription isothermique permettant de detecter et de determiner le genotype du virus de dengue
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection
WO1999034020A1 (fr) * 1997-12-31 1999-07-08 Akzo Nobel N.V. Dosage base sur la transcription isothermique permettant de detecter et de determiner le genotype du virus de dengue

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1994, PIERRE V ET AL: "Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction.", XP002222878, Database accession no. PREV199497336271 *
RESEARCH IN VIROLOGY, vol. 145, no. 2, 1994, pages 93 - 104, ISSN: 0923-2516 *
See also references of EP1276898A1 *

Also Published As

Publication number Publication date
EP1276898A1 (fr) 2003-01-22
CA2405960A1 (fr) 2001-10-25
WO2001079546A2 (fr) 2001-10-25
BR0017215A (pt) 2004-02-10
AU2001212172A1 (en) 2001-10-30

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