WO2001079546A3 - Flavivirus detection and quantification assay - Google Patents

Flavivirus detection and quantification assay Download PDF

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Publication number
WO2001079546A3
WO2001079546A3 PCT/US2000/028961 US0028961W WO0179546A3 WO 2001079546 A3 WO2001079546 A3 WO 2001079546A3 US 0028961 W US0028961 W US 0028961W WO 0179546 A3 WO0179546 A3 WO 0179546A3
Authority
WO
WIPO (PCT)
Prior art keywords
flavivirus
fluorogenic
disclosed
permit
dengue virus
Prior art date
Application number
PCT/US2000/028961
Other languages
French (fr)
Other versions
WO2001079546A2 (en
Inventor
Huo-Shu H Houng
Niranjan Kanesa-Thasan
Original Assignee
Us Army Med Res Mat Command
Huo-Shu H Houng
Niranjan Kanesa-Thasan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/551,161 external-priority patent/US6793488B1/en
Application filed by Us Army Med Res Mat Command, Huo-Shu H Houng, Niranjan Kanesa-Thasan filed Critical Us Army Med Res Mat Command
Priority to EP00973687A priority Critical patent/EP1276898A1/en
Priority to AU2001212172A priority patent/AU2001212172A1/en
Priority to BR0017215-4A priority patent/BR0017215A/en
Priority to CA002405960A priority patent/CA2405960A1/en
Publication of WO2001079546A2 publication Critical patent/WO2001079546A2/en
Publication of WO2001079546A3 publication Critical patent/WO2001079546A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Fluorescent DNA probes specific and flanking primer pairs are designed based on the sequence information found in the conserved terminal 3'-noncoding region of flavivirus, e.g. nucleotides 10653-10678 of dengue virus. Fluorogenic polymerase chain reaction employing these primers and probes produce results that permit specific flavivirus identification. The assays can be both quantitative and qualitative. Optimal assay conditions with zero background are disclosed which permit the detection of low levels of flavivirus from clinical specimens. Specifically, Dengue virus isolates from different geographic regions can be universally detected and identified by the disclosed fluorogenic RT-PCR assay. The fluorogenic RT-PCR assay readily detected viremia in sera collected from individuals ill with dengue fever.
PCT/US2000/028961 2000-04-14 2000-10-19 Flavivirus detection and quantification assay WO2001079546A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00973687A EP1276898A1 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay
AU2001212172A AU2001212172A1 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay
BR0017215-4A BR0017215A (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification analysis
CA002405960A CA2405960A1 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/551,161 US6793488B1 (en) 1999-04-16 2000-04-14 Flavivirus detection and quantification assay
US09/551,161 2000-04-14

Publications (2)

Publication Number Publication Date
WO2001079546A2 WO2001079546A2 (en) 2001-10-25
WO2001079546A3 true WO2001079546A3 (en) 2003-06-05

Family

ID=24200105

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028961 WO2001079546A2 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay

Country Status (5)

Country Link
EP (1) EP1276898A1 (en)
AU (1) AU2001212172A1 (en)
BR (1) BR0017215A (en)
CA (1) CA2405960A1 (en)
WO (1) WO2001079546A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
EP2366803B8 (en) * 2002-10-16 2015-08-19 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
AU2008200221B2 (en) * 2002-10-16 2012-04-05 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
WO2013080307A1 (en) * 2011-11-29 2013-06-06 株式会社 東芝 Primer set for amplifying mosquito-borne virus, assay kit for detecting mosquito-borne virus, and detection method using said primer set and said assay kit
CN104342498B (en) * 2013-07-30 2019-04-16 中国疾病预防控制中心病毒病预防控制所 Culex flavivirus real-time fluorescence quantitative RT-PCR detection method and kit
CN104357582B (en) * 2014-10-31 2017-02-15 中国检验检疫科学研究院 Real-time fluorescence RT-PCR detection kit and method of Kunjin virus
CN107893128A (en) * 2017-11-09 2018-04-10 贵州医科大学 The primer pair combination, probe and application of 10 kinds of arboviruses of detection simultaneously
CN110846441B (en) * 2019-12-20 2023-04-11 浙江省淡水水产研究所 Specific primer, probe and rapid detection kit for detecting macrobrachium flaviviridae-1

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999034020A1 (en) * 1997-12-31 1999-07-08 Akzo Nobel N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection
WO1999034020A1 (en) * 1997-12-31 1999-07-08 Akzo Nobel N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1994, PIERRE V ET AL: "Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction.", XP002222878, Database accession no. PREV199497336271 *
RESEARCH IN VIROLOGY, vol. 145, no. 2, 1994, pages 93 - 104, ISSN: 0923-2516 *
See also references of EP1276898A1 *

Also Published As

Publication number Publication date
CA2405960A1 (en) 2001-10-25
EP1276898A1 (en) 2003-01-22
WO2001079546A2 (en) 2001-10-25
BR0017215A (en) 2004-02-10
AU2001212172A1 (en) 2001-10-30

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