WO2001012783A3 - Motile sperm separation by cellulose derivatives - Google Patents

Motile sperm separation by cellulose derivatives Download PDF

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Publication number
WO2001012783A3
WO2001012783A3 PCT/GB2000/003130 GB0003130W WO0112783A3 WO 2001012783 A3 WO2001012783 A3 WO 2001012783A3 GB 0003130 W GB0003130 W GB 0003130W WO 0112783 A3 WO0112783 A3 WO 0112783A3
Authority
WO
WIPO (PCT)
Prior art keywords
sperm
motile sperm
medium
cellulose derivatives
sperm separation
Prior art date
Application number
PCT/GB2000/003130
Other languages
French (fr)
Other versions
WO2001012783A2 (en
Inventor
Christopher Lowther Ro Barratt
David Mortimer
Original Assignee
Genosis Ltd
Christopher Lowther Ro Barratt
David Mortimer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genosis Ltd, Christopher Lowther Ro Barratt, David Mortimer filed Critical Genosis Ltd
Priority to AU64605/00A priority Critical patent/AU6460500A/en
Publication of WO2001012783A2 publication Critical patent/WO2001012783A2/en
Publication of WO2001012783A3 publication Critical patent/WO2001012783A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Methods and products for separating motile sperm cells in vitro from a sperm-containing sample by means of contacting said sample with a layer of an aqueous artificial penetration medium for sperm cells, said medium haivng incorporated therein a cellulose derivative, whereby motile sperm are caused to migrate into said layer from said sperm containing sample. The process of separating motile sperm is improved if the penetration medium contains a cellulose derivative, such as a methylcellulose or a hydroxymethylcellulose. The improved medium is inexpensive compared with hyaluronic acid, yet dependable and effective.
PCT/GB2000/003130 1999-08-16 2000-08-15 Motile sperm separation by cellulose derivatives WO2001012783A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU64605/00A AU6460500A (en) 1999-08-16 2000-08-15 Motile sperm separation by cellulose derivatives

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9919370.8 1999-08-16
GBGB9919370.8A GB9919370D0 (en) 1999-08-16 1999-08-16 Method

Publications (2)

Publication Number Publication Date
WO2001012783A2 WO2001012783A2 (en) 2001-02-22
WO2001012783A3 true WO2001012783A3 (en) 2001-08-30

Family

ID=10859251

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003130 WO2001012783A2 (en) 1999-08-16 2000-08-15 Motile sperm separation by cellulose derivatives

Country Status (3)

Country Link
AU (1) AU6460500A (en)
GB (1) GB9919370D0 (en)
WO (1) WO2001012783A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101487841B (en) * 2009-02-13 2014-06-04 深圳市人民医院 Coating carrier and its use in method for sperm maturity detection and non-invasive mature sperm separation
US9546935B1 (en) 2010-08-25 2017-01-17 Thomas W. Astle Dried specimen storage slides, systems and methods
US9575084B1 (en) 2010-08-25 2017-02-21 Thomas W. Astle Dried specimen storage slide
US20130029312A1 (en) * 2011-07-27 2013-01-31 Biocoat, Inc. Integral sperm preparation for intracytoplasmic sperm injection

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009648A1 (en) * 1998-08-14 2000-02-24 Genosis Limited Separation and detection of spermatozoa

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009648A1 (en) * 1998-08-14 2000-02-24 Genosis Limited Separation and detection of spermatozoa

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ENGLAND G C W ET AL: "EVALUATION OF CELLULOSE ACETATE NITRATE FILTERS FOR MEASURING THE MOTILITY OF DOG SPERMATOZOA", JOURNAL OF REPRODUCTION AND FERTILITY, vol. 88, no. 1, 1990, pages 369 - 374, XP000982155, ISSN: 0022-4251 *
MORTIMER D (REPRINT): "HUMAN- SPERM MOVEMENT IN SPERM -SELECT AND HIGH-VISCOSITY METHYL CELLULOSE SOLUTION", DEVELOPMENT GROWTH & DIFFERENTIATION, (1986) VOL. 28, PP. 90., UNIV CALGARY, HLTH SCI CTR, ENDOCRINE INFERTIL CLIN, CALGARY T2N 1N4, ALBERTA, CANADA, XP000982182 *
STRZEMIENSKI P J ET AL: "Evaluation of cellulose acetate/nitrate filters for the study of stallion sperm motility.", JOURNAL OF REPRODUCTION AND FERTILITY. SUPPLEMENT, (1987) 35 33-8., XP000982156 *

Also Published As

Publication number Publication date
GB9919370D0 (en) 1999-10-20
AU6460500A (en) 2001-03-13
WO2001012783A2 (en) 2001-02-22

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