WO1999058643A8 - Micro-organisme produisant de l'acide docosahexanoique et une phospholipase a1, milieu de culture liquide de ce micro-organisme, procede de preparation d'un phospholipide, et procede de production de 2-acyllysophospholipide - Google Patents

Micro-organisme produisant de l'acide docosahexanoique et une phospholipase a1, milieu de culture liquide de ce micro-organisme, procede de preparation d'un phospholipide, et procede de production de 2-acyllysophospholipide

Info

Publication number
WO1999058643A8
WO1999058643A8 PCT/JP1999/002446 JP9902446W WO9958643A8 WO 1999058643 A8 WO1999058643 A8 WO 1999058643A8 JP 9902446 W JP9902446 W JP 9902446W WO 9958643 A8 WO9958643 A8 WO 9958643A8
Authority
WO
WIPO (PCT)
Prior art keywords
producing
culture medium
liquid culture
docosahexaenoic acid
acyllysophospholipid
Prior art date
Application number
PCT/JP1999/002446
Other languages
English (en)
Japanese (ja)
Other versions
WO1999058643A1 (fr
Inventor
Kazunaga Yazawa
Kazuo Watanabe
Masazumi Kamata
Miho Tomita
Hiroshi Muramatsu
Original Assignee
Sagami Chem Res
Kazunaga Yazawa
Kazuo Watanabe
Masazumi Kamata
Miho Tomita
Hiroshi Muramatsu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sagami Chem Res, Kazunaga Yazawa, Kazuo Watanabe, Masazumi Kamata, Miho Tomita, Hiroshi Muramatsu filed Critical Sagami Chem Res
Priority to AU37290/99A priority Critical patent/AU3729099A/en
Publication of WO1999058643A1 publication Critical patent/WO1999058643A1/fr
Publication of WO1999058643A8 publication Critical patent/WO1999058643A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un micro-organisme appartenant au genre Psychrobacter et produisant de l'acide docosahexanoïque. L'invention concerne également le milieu de culture liquide de ce micro-organisme, ce milieu de culture liquide présentant une action de la phospholipase A1. L'invention concerne en outre un procédé de préparation d'un phospholipide riche en acide docosahexanoïque, ce procédé utilisant le milieu de culture liquide susmentionné. L'invention concerne enfin un procédé de production de 2-acyllysophospholipide consistant à hydrolyser un phospholipide à l'aide dudit milieu de culture liquide.
PCT/JP1999/002446 1998-05-14 1999-05-12 Micro-organisme produisant de l'acide docosahexanoique et une phospholipase a1, milieu de culture liquide de ce micro-organisme, procede de preparation d'un phospholipide, et procede de production de 2-acyllysophospholipide WO1999058643A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU37290/99A AU3729099A (en) 1998-05-14 1999-05-12 Microorganism producing docosahexaenoic acid and phospholipase a1, liquid culture medium thereof, process for preparing phospholipid and process for producing 2-acyllysophospholipid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP10131515A JPH11318434A (ja) 1998-05-14 1998-05-14 ドコサヘキサエン酸及びホスホリパーゼａ１を産生する微生物、ドコサヘキサエン酸の製造方法、微生物の培養液、ドコサヘキサエン酸高含有リン脂質の調製法及び２−アシルリゾリン脂質の製造法
JP10/131515 1998-05-14

Publications (2)

Publication Number Publication Date
WO1999058643A1 WO1999058643A1 (fr) 1999-11-18
WO1999058643A8 true WO1999058643A8 (fr) 2000-02-17

Family

ID=15059856

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1999/002446 WO1999058643A1 (fr) 1998-05-14 1999-05-12 Micro-organisme produisant de l'acide docosahexanoique et une phospholipase a1, milieu de culture liquide de ce micro-organisme, procede de preparation d'un phospholipide, et procede de production de 2-acyllysophospholipide

Country Status (3)

Country Link
JP (1) JPH11318434A (fr)
AU (1) AU3729099A (fr)
WO (1) WO1999058643A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1995306A4 (fr) 2006-02-27 2009-12-23 Tokyo University Of Marine Sci Nouveau microbe, agent de modification de lipide, procédé de production de 2-acyl-lysophospholipide, procédé de production de diacylglycérol, procédé de production de céramide et procédé de dégommage d'huile ou de graisse

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10323182A (ja) * 1997-03-27 1998-12-08 Sagami Chem Res Center ホスホリパーゼａ１活性を有する細菌の培養液、ドコサヘキサエン酸高含有リン脂質の調製法及び２−アシルリゾリン脂質の製造法

Also Published As

Publication number Publication date
JPH11318434A (ja) 1999-11-24
WO1999058643A1 (fr) 1999-11-18
AU3729099A (en) 1999-11-29

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