WO1998045700A3 - Method for the mass spectrometric sequencing of biopolymers - Google Patents

Method for the mass spectrometric sequencing of biopolymers Download PDF

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Publication number
WO1998045700A3
WO1998045700A3 PCT/DE1998/001016 DE9801016W WO9845700A3 WO 1998045700 A3 WO1998045700 A3 WO 1998045700A3 DE 9801016 W DE9801016 W DE 9801016W WO 9845700 A3 WO9845700 A3 WO 9845700A3
Authority
WO
WIPO (PCT)
Prior art keywords
biopolymers
mass
sequencing
fragments
separation
Prior art date
Application number
PCT/DE1998/001016
Other languages
German (de)
French (fr)
Other versions
WO1998045700A2 (en
Inventor
Joachim W Engels
Karlheinz Woerner
Konrad Faulstich
Hannelore Brill
Original Assignee
Joachim W Engels
Karlheinz Woerner
Konrad Faulstich
Hannelore Brill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joachim W Engels, Karlheinz Woerner, Konrad Faulstich, Hannelore Brill filed Critical Joachim W Engels
Publication of WO1998045700A2 publication Critical patent/WO1998045700A2/en
Publication of WO1998045700A3 publication Critical patent/WO1998045700A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Abstract

The invention relates to a new method for sequencing biopolymers by mass spectrometry. The sequencing of biopolymers is either lengthy or requires exact determination of the mass of the fragments, which is difficult, especially in the case of long polymers. The speed of hydrolysis of phosphodiester, -peptide or -glycoside bonds with exo/endonucleases, -peptidases, -glycosidases or other hydrolytically acting substances is used in the inventive method for the sequence analysis of nucleic acids or other biopolymers. Separation and detection of the fragments produced take place by mass spectrometry by determining the mass and the different peak intensities. The main advantage of the method is that an exact determination of the mass is no longer necessary, and also that low-resolution mass spectrometers can be used. Furthermore, analysis and separation of fragments is extremely rapid, as there is no electrophoresis and the sequence of modified nucleic acids can be determined. These principles can also be applied to sequencing or secondary structure determination of other biopolymers, like, for example, peptides and oligosaccharides.
PCT/DE1998/001016 1997-04-09 1998-04-08 Method for the mass spectrometric sequencing of biopolymers WO1998045700A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19714558.2 1997-04-09
DE19714558A DE19714558A1 (en) 1997-04-09 1997-04-09 A new method for sequencing biopolymers using mass spectrometry

Publications (2)

Publication Number Publication Date
WO1998045700A2 WO1998045700A2 (en) 1998-10-15
WO1998045700A3 true WO1998045700A3 (en) 1999-03-11

Family

ID=7825845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/001016 WO1998045700A2 (en) 1997-04-09 1998-04-08 Method for the mass spectrometric sequencing of biopolymers

Country Status (2)

Country Link
DE (1) DE19714558A1 (en)
WO (1) WO1998045700A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994969B1 (en) 1999-04-30 2006-02-07 Methexis Genomics, N.V. Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
WO2000071755A2 (en) * 1999-05-25 2000-11-30 Praelux Incorporated Method for sequency and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
WO2001094910A2 (en) * 2000-06-07 2001-12-13 Basf Aktiengesellschaft Method for the qualitative and quantitative analysis of complex mixtures of chemical compounds, using maldi-tof mass spectrometry
DE102008007112B3 (en) * 2008-01-31 2009-08-06 Johann Wolfgang Goethe-Universität Frankfurt am Main Method for sequencing an RNA molecule by mass spectrometry

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004596A1 (en) * 1988-10-28 1990-05-03 3I Research Exploitation Limited Isolation of oligosaccharides
WO1994021822A1 (en) * 1993-03-19 1994-09-29 Sequenom, Inc. Dna sequencing by mass spectrometry via exonuclease degradation
WO1996036986A1 (en) * 1995-05-19 1996-11-21 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1998003684A1 (en) * 1996-07-19 1998-01-29 Hybridon, Inc. Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
WO1998020166A2 (en) * 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004596A1 (en) * 1988-10-28 1990-05-03 3I Research Exploitation Limited Isolation of oligosaccharides
WO1994021822A1 (en) * 1993-03-19 1994-09-29 Sequenom, Inc. Dna sequencing by mass spectrometry via exonuclease degradation
WO1996036986A1 (en) * 1995-05-19 1996-11-21 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
WO1997033000A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1998003684A1 (en) * 1996-07-19 1998-01-29 Hybridon, Inc. Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
WO1998020166A2 (en) * 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WOERNER K ET AL: "RECENT DEVELOPMENTS IN MASS SPECTROMETRY: RNA SEQUENCING", NUCLEOSIDES & NUCLEOTIDES, vol. 16, no. 5/06, 1997, pages 573 - 577, XP000198057 *

Also Published As

Publication number Publication date
WO1998045700A2 (en) 1998-10-15
DE19714558A1 (en) 1998-10-15

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