WO1996011401A1 - Colorant supravital contenant un fixateur pour cellules - Google Patents
Colorant supravital contenant un fixateur pour cellules Download PDFInfo
- Publication number
- WO1996011401A1 WO1996011401A1 PCT/JP1995/001937 JP9501937W WO9611401A1 WO 1996011401 A1 WO1996011401 A1 WO 1996011401A1 JP 9501937 W JP9501937 W JP 9501937W WO 9611401 A1 WO9611401 A1 WO 9611401A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- stainer
- supravital
- staining
- containing cell
- cell
- Prior art date
Links
- 241001510071 Pyrrhocoridae Species 0.000 title abstract 4
- 239000000834 fixative Substances 0.000 title 1
- 206010018910 Haemolysis Diseases 0.000 abstract description 5
- 210000003743 erythrocyte Anatomy 0.000 abstract description 5
- 230000008588 hemolysis Effects 0.000 abstract description 5
- 230000004660 morphological change Effects 0.000 abstract description 5
- 210000003850 cellular structure Anatomy 0.000 abstract description 4
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract description 3
- 230000003204 osmotic effect Effects 0.000 abstract description 3
- 238000010186 staining Methods 0.000 abstract description 3
- 238000007447 staining method Methods 0.000 abstract description 2
- 150000001299 aldehydes Chemical class 0.000 abstract 1
- 238000004043 dyeing Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 238000004040 coloring Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960000587 glutaral Drugs 0.000 description 2
- 208000006750 hematuria Diseases 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Definitions
- This invention is directed to a super-organic stained liquid comprising a cell-fixing agent in a body fluid of a living body, in particular, a coloring liquid for dyeing tangible components such as cells in urine.
- a super-organic stained liquid comprising a cell-fixing agent in a body fluid of a living body, in particular, a coloring liquid for dyeing tangible components such as cells in urine.
- the conventional supracellular dyeing liquid is one that dyes in a fresh state to fix cell components. Therefore, the cells in the sample, especially erythrocytes, are directly affected by the pH and osmotic pressure of the dye solution itself, resulting in marked hemolysis and morphological changes. In other words, the differentiation of medical hematuria and urological hematuria by erythrocyte morphology was a key issue. In addition, the conventional supra-organism color sample was difficult to preserve even due to the 55-cell bacterial growth and the temporal change of the cells.
- the present invention impairs the advantages of dyeing cells and easiness of dyeing super-organisms.
- the subject of this research is to prevent the hemolysis and shape change of red blood cells due to the above (maintaining the fine structure).
- the microstructure of the cell membrane In order to prevent hemolysis and morphological changes due to staining, the microstructure of the cell membrane must be firmly fixed. As a result of experimentation and theory, it was found that the solidifying agent was glutar aldehyde to a final concentration of 0.5% after dyeing as a result of experimentation and theory. It was discovered that adding to the supra-organism dye solution was the best.
- the erythrocytes depend on the coloring liquid because the microstructure of the cell membrane is firmly fixed.
- the present invention has the above composition, the cell components are fixed, and the morphological changes due to the osmotic pressure of the coloring liquid and the effect of the pH. In addition to being able to prevent this from happening, it has become possible to save the sample as it is.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
L'invention porte sur un colorant supravital pouvant résoudre des problèmes tels que ceux qu'on rencontre dans les procédés usuels de coloration supravitale dus au fait que les composants de la cellule, en particulier les érythrocytes, sont influencés par la pression osmotique ou le pH du colorant et en subissent une hémolyse ou des modifications morphologiques qui en rendent la conservation difficile. On obtient ledit colorant en ajoutant du glutaraldéhyde au colorant supravital de façon que la concentration finale en aldéhyde reste de 0,5 % après la coloration, ce qui permet d'effectuer simultanément la coloration et la fixation des composants de la cellule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU35343/95A AU3534395A (en) | 1994-10-08 | 1995-09-25 | Supravital stainer containing cell fixative |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28114694A JPH09105744A (ja) | 1994-10-08 | 1994-10-08 | 細胞固定剤入り超生体染色液 |
| JP6/281146 | 1994-10-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1996011401A1 true WO1996011401A1 (fr) | 1996-04-18 |
Family
ID=17635009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP1995/001937 WO1996011401A1 (fr) | 1994-10-08 | 1995-09-25 | Colorant supravital contenant un fixateur pour cellules |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPH09105744A (fr) |
| AU (1) | AU3534395A (fr) |
| WO (1) | WO1996011401A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114295452A (zh) * | 2021-12-31 | 2022-04-08 | 力因精准医疗产品（上海）有限公司 | 一种人***形态学染色试剂及染色方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6179163A (ja) * | 1984-09-26 | 1986-04-22 | Toa Medical Electronics Co Ltd | 血球測定用試薬 |
| JPH03140864A (ja) * | 1989-10-27 | 1991-06-14 | Mitsubishi Kasei Corp | 網状赤血球計数装置 |
-
1994
- 1994-10-08 JP JP28114694A patent/JPH09105744A/ja active Pending
-
1995
- 1995-09-25 WO PCT/JP1995/001937 patent/WO1996011401A1/fr active Application Filing
- 1995-09-25 AU AU35343/95A patent/AU3534395A/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6179163A (ja) * | 1984-09-26 | 1986-04-22 | Toa Medical Electronics Co Ltd | 血球測定用試薬 |
| JPH03140864A (ja) * | 1989-10-27 | 1991-06-14 | Mitsubishi Kasei Corp | 網状赤血球計数装置 |
Non-Patent Citations (1)
| Title |
|---|
| STAIN TECHNOLOGY, Vol. 46, No. 4, 1971, V. AUGULIS, E.B. SIGG, "Supravital Staining and Fixation of Brain and Spinal Cord by Intravascular Perfusion", pages 183-190. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114295452A (zh) * | 2021-12-31 | 2022-04-08 | 力因精准医疗产品（上海）有限公司 | 一种人***形态学染色试剂及染色方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU3534395A (en) | 1996-05-02 |
| JPH09105744A (ja) | 1997-04-22 |
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