WO1991012273A3 - Procedes de production de recepteurs hybrides couples par proteines g - Google Patents

Procedes de production de recepteurs hybrides couples par proteines g Download PDF

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Publication number
WO1991012273A3
WO1991012273A3 PCT/US1991/000909 US9100909W WO9112273A3 WO 1991012273 A3 WO1991012273 A3 WO 1991012273A3 US 9100909 W US9100909 W US 9100909W WO 9112273 A3 WO9112273 A3 WO 9112273A3
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WO
WIPO (PCT)
Prior art keywords
protein
domain
coupled
methods
coupled receptors
Prior art date
Application number
PCT/US1991/000909
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English (en)
Other versions
WO1991012273A2 (fr
Inventor
Andrzej Z Sledziewski
Paul O Sheppard
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to EP91904758A priority Critical patent/EP0594594B1/fr
Priority to JP50556091A priority patent/JP3333506B2/ja
Priority to CA002074837A priority patent/CA2074837C/fr
Priority to DE69125953T priority patent/DE69125953T2/de
Publication of WO1991012273A2 publication Critical patent/WO1991012273A2/fr
Publication of WO1991012273A3 publication Critical patent/WO1991012273A3/fr
Priority to GR970401545T priority patent/GR3023898T3/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Procédés de production de récepteurs hybrides couplés par des protéines G. On a obtenu des séquences d'ADN codant des récepteurs hybrides couplés par des protéines G dans lesquelles les récepteurs comprennent des récepteurs mammifères couplés par des protéines G possédant au moins un domaine différent du domaine de liaison de ligands remplacé par un domaine correspondant d'un récepteur de levure couplé par protéine G. Les structures D'ADN comprennent les éléments liés fonctionnellement suivants: un promoteur de transcription, une séquence d'ADN codant un récepteur hybride couplé par des protéines G, le récepteur comprenant un récepteur mammifère couplé par protéine G possédant au moins un domaine différent du domaine de liaison de ligand remplacé par un domaine correspodant d'un récepteur de levure couplé par des protéines G, ainsi qu'une terminaison de transcription. L'invention concerne également des cellules hôtes transformées à l'aide des structures d'ADN ainsi que des procédés utilisant les cellules transformées.
PCT/US1991/000909 1990-02-08 1991-02-08 Procedes de production de recepteurs hybrides couples par proteines g WO1991012273A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP91904758A EP0594594B1 (fr) 1990-02-08 1991-02-08 Procedes de production de recepteurs hybrides couples par proteines g
JP50556091A JP3333506B2 (ja) 1990-02-08 1991-02-08 ハイブリッドｇタンパク質結合レセプターの製造方法
CA002074837A CA2074837C (fr) 1990-02-08 1991-02-08 Methodes permettant de produire des recepteurs hybrides couples a la proteine g
DE69125953T DE69125953T2 (de) 1990-02-08 1991-02-08 Herstellungsmethode für hybride, g-proteingekoppelte rezeptoren
GR970401545T GR3023898T3 (en) 1990-02-08 1997-06-26 Methods of producing hybrid g protein-coupled receptors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/478,100 US5284746A (en) 1990-02-08 1990-02-08 Methods of producing hybrid G protein-coupled receptors
US478,100 1990-02-08

Publications (2)

Publication Number Publication Date
WO1991012273A2 WO1991012273A2 (fr) 1991-08-22
WO1991012273A3 true WO1991012273A3 (fr) 1991-10-03

Family

ID=23898515

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/000909 WO1991012273A2 (fr) 1990-02-08 1991-02-08 Procedes de production de recepteurs hybrides couples par proteines g

Country Status (14)

Country Link
US (2) US5284746A (fr)
EP (1) EP0594594B1 (fr)
JP (1) JP3333506B2 (fr)
AT (1) ATE152479T1 (fr)
AU (1) AU7333691A (fr)
CA (1) CA2074837C (fr)
CS (1) CS31391A2 (fr)
DE (1) DE69125953T2 (fr)
DK (1) DK0594594T3 (fr)
ES (1) ES2100947T3 (fr)
GR (1) GR3023898T3 (fr)
IE (1) IE63987B1 (fr)
PT (1) PT96714B (fr)
WO (1) WO1991012273A2 (fr)

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US6100042A (en) * 1993-03-31 2000-08-08 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US7416881B1 (en) * 1993-03-31 2008-08-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US6255059B1 (en) 1993-03-31 2001-07-03 Cadus Pharmaceutical Corporation Methods for identifying G protein coupled receptor effectors
AU685103B2 (en) * 1993-03-31 1998-01-15 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US5691188A (en) * 1994-02-14 1997-11-25 American Cyanamid Company Transformed yeast cells expressing heterologous G-protein coupled receptor
US6537764B1 (en) * 1995-01-19 2003-03-25 Children's Medical Center Corporation Method of identifying inhibitors of C—C chemokine receptor 3
US6806061B1 (en) 1995-01-19 2004-10-19 Children's Medical Center Corporation G protein-coupled receptor gene and methods of use therefor
US6881545B1 (en) 1995-12-28 2005-04-19 Takeda Chemical Industries, Ltd. Polypeptides, their production and use
EP0870020B1 (fr) 1995-12-28 2006-08-09 Takeda Pharmaceutical Company Limited Polypeptides formant ligands vis-a-vis de la proteine receptrice pituitaire liee a la proteine g-, leur fabrication et utilisation
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
US5891646A (en) * 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US6528271B1 (en) * 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US6355473B1 (en) 1998-05-06 2002-03-12 Cadus Pharmaceutical Corp. Yeast cells having mutations in stp22 and uses therefor
US6692696B1 (en) * 1998-06-18 2004-02-17 ARETé ASSOCIATES Biosensor
US6312689B1 (en) 1998-07-23 2001-11-06 Millennium Pharmaceuticals, Inc. Anti-CCR2 antibodies and methods of use therefor
US7081360B2 (en) 1998-07-28 2006-07-25 Cadus Technologies, Inc. Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties
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US6251605B1 (en) 1998-10-27 2001-06-26 Cadus Pharmaceutical Corporation Yeast cells having mutations in Cav1 and uses therefor
WO2000031261A2 (fr) 1998-11-25 2000-06-02 Cadus Pharmaceutical Corporation Methodes et compositions d'identification d'effecteurs de recepteurs
US6504008B1 (en) 1999-02-01 2003-01-07 Cadus Technologies, Inc. Cell based signal generation
AU5330000A (en) * 1999-06-08 2000-12-28 Regents Of The University Of California, The Methods and compositions for identifying inhibitors of osteoclastic bone reabsorption
US6555325B1 (en) 1999-06-14 2003-04-29 Cadus Technologies, Inc. System for detection of a functional interaction between a compound and a cellular signal transduction component
US7223533B2 (en) 1999-09-10 2007-05-29 Cadus Technologies, Inc. Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways
US7324236B2 (en) * 2000-02-01 2008-01-29 Canon Kabushiki Kaisha Discrepancy correction method and apparatus for correcting difference in levels of image signals obtained by an image sensor having a multiple output channels
CA2420325A1 (fr) 2000-08-25 2002-02-28 Basf Plant Science Gmbh Polynucleotides vegetaux codant de nouvelles proteases prenyle
US20050136431A1 (en) * 2000-11-03 2005-06-23 Oakley Robert H. Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
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EP1326076A3 (fr) * 2001-10-26 2004-01-28 Millenium Pharmaceuticals, Inc. Méthode d'identification de ligands ou modulateurs du récepteur 2871, un récepteur couplé aux protéine g
CA2476773C (fr) 2002-01-25 2013-05-28 G2 Therapies Ltd Anticorps monoclonaux agissant contre des boucles extracellulaires de c5ar
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CA2649295A1 (fr) * 2006-04-21 2007-11-01 Wyeth Procedes de criblage a haut debit de lignees cellulaires
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AU2009217224B2 (en) 2008-02-20 2014-09-11 G2 Inflammation Pty Ltd Humanized anti-C5aR antibodies
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Also Published As

Publication number Publication date
EP0594594B1 (fr) 1997-05-02
PT96714B (pt) 1998-07-31
DK0594594T3 (da) 1997-09-29
CA2074837C (fr) 2001-11-27
JP3333506B2 (ja) 2002-10-15
ES2100947T3 (es) 1997-07-01
DE69125953D1 (de) 1997-06-05
ATE152479T1 (de) 1997-05-15
PT96714A (pt) 1991-10-31
US5284746A (en) 1994-02-08
CA2074837A1 (fr) 1991-08-09
JPH05504258A (ja) 1993-07-08
GR3023898T3 (en) 1997-09-30
IE63987B1 (en) 1995-06-28
CS31391A2 (en) 1991-11-12
AU7333691A (en) 1991-09-03
IE910430A1 (en) 1991-08-14
WO1991012273A2 (fr) 1991-08-22
US5576210A (en) 1996-11-19
DE69125953T2 (de) 1997-08-21
EP0594594A1 (fr) 1994-05-04

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