WO1990009449A3 - Systeme de transformation du candida tropicalis - Google Patents

Systeme de transformation du candida tropicalis Download PDF

Info

Publication number
WO1990009449A3
WO1990009449A3 PCT/US1990/000737 US9000737W WO9009449A3 WO 1990009449 A3 WO1990009449 A3 WO 1990009449A3 US 9000737 W US9000737 W US 9000737W WO 9009449 A3 WO9009449 A3 WO 9009449A3
Authority
WO
WIPO (PCT)
Prior art keywords
transformation system
microorganisms
constructs
candida tropicalis
auxotrophic
Prior art date
Application number
PCT/US1990/000737
Other languages
English (en)
Other versions
WO1990009449A2 (fr
Inventor
James Michael Cregg
Martin Anthony Gleeson
Lisa Haas
Stephen Picataggio
Original Assignee
Henkel Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel Research Corp filed Critical Henkel Research Corp
Publication of WO1990009449A2 publication Critical patent/WO1990009449A2/fr
Publication of WO1990009449A3 publication Critical patent/WO1990009449A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/924—Candida tropicalis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

On décrit un système de transformation du C. Tropicalis, comprenant des structures et des microorganismes, ainsi que des procédés permettant de préparer ces structures et microorganismes et de transformer lesdits microorganismes. On décrit notamment, un système de transformation à levure comprenant des hôtes auxotrophes, qui sont auxotrophes pour la voie d'acides aminés, celle de purine ou celle de pyrimidine, et utilisant des structures d'ADN comportant des gènes codant pour des enzymes biosynthétiques qui complètent de manière fonctionnelle les auxotrophies pour les rendre prototrophiques.
PCT/US1990/000737 1989-02-08 1990-02-08 Systeme de transformation du candida tropicalis WO1990009449A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30848189A 1989-02-08 1989-02-08
US308,481 1989-02-08
US386,837 1989-07-27
US07/386,837 US5204252A (en) 1989-02-08 1989-07-27 Candida tropicalis transformation system

Publications (2)

Publication Number Publication Date
WO1990009449A2 WO1990009449A2 (fr) 1990-08-23
WO1990009449A3 true WO1990009449A3 (fr) 1991-07-11

Family

ID=26976272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/000737 WO1990009449A2 (fr) 1989-02-08 1990-02-08 Systeme de transformation du candida tropicalis

Country Status (5)

Country Link
US (1) US5204252A (fr)
EP (1) EP0457852A1 (fr)
JP (1) JPH04505557A (fr)
CA (1) CA2046641A1 (fr)
WO (1) WO1990009449A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254466A (en) * 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
CU22722A1 (es) * 1996-10-03 2002-02-28 Ct Ingenieria Genetica Biotech Sistema de transformación para la expresión de genes heterólogos en la levadura candida utilis
DK2942391T3 (en) 2004-08-13 2018-08-27 Univ Pennsylvania PROCEDURES FOR THE MANUFACTURE OF ANTIBIOTICS-RESISTANCE-FREE VACCINES
US20120156761A1 (en) 2009-07-02 2012-06-21 Verdezyne, Inc. Biological methods for preparing adipic acid
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
WO2019014309A1 (fr) 2017-07-13 2019-01-17 Verdezyne (Abc), Llc Procédés biologiques pour modifier un flux de carbone cellulaire
CN112680369B (zh) * 2019-10-18 2024-05-03 上海凯赛生物技术股份有限公司 一种高产长链二元酸的菌株及其发酵方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221455A1 (fr) * 1985-10-25 1987-05-13 Phillips Petroleum Company Gène d'argininosuccinate lyase de Pichia pastoris et son utilisation

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU204097B (en) * 1982-05-19 1991-11-28 Gist Brocades Nv Process for producing cloning system relating to kluyveromyces species
IL76190A0 (en) * 1984-08-31 1985-12-31 Smithkline Beckman Corp Recombinant saccharomyces
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4735901A (en) * 1985-01-14 1988-04-05 E. R. Squibb & Sons, Inc. Transformation of Candida albicans
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221455A1 (fr) * 1985-10-25 1987-05-13 Phillips Petroleum Company Gène d'argininosuccinate lyase de Pichia pastoris et son utilisation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Biotechnology and Genetic Engineering Reviews, volume 4, September 1986, Intercept Ltd, (Ponteland, Newcastle upon Tyne, GB), S.L. Sturley et al.: "Genetic manipulation of commercial yeast strains", pages 1-37 *
Chemical Abstracts, volume 102, no. 15, 15 April 1985, (Columbus, Ohio, US), J.C. Loper et al.: "Engineering genes in yeast for biodegradations", see page 165 *
Chemical Abstracts, volume 108, no. 15, 11 April 1988, (Columbus, Ohio, US), G.I. Golovina et al.: "Use of intergeneric fusions to identify mutations in the asporogenous yeast Candida tropicals", see page 349 *
Chemical Abstracts, volume 108, no. 15, 11 April 1988, (Columbus, Ohio, US), W.K. Ki et al.: "Studies on yeast vector system for eukaryotic gene cloning. New cloning vector containing autonomous replicating segment from Candida tropicalis", see page 193 *
Chemical Abstracts, volume 96, no. 5, 1 February 1982, (Columbus, Ohio, US), I.I. Danilenko: "Action of exogenous DNA on the Candida tropicalis D-2 mutant having a histidine deficiency and a varying amount of intracellular lipids", see page 333 *

Also Published As

Publication number Publication date
WO1990009449A2 (fr) 1990-08-23
CA2046641A1 (fr) 1990-08-09
EP0457852A1 (fr) 1991-11-27
JPH04505557A (ja) 1992-10-01
US5204252A (en) 1993-04-20

Similar Documents

Publication Publication Date Title
EP0188677A3 (fr) Gènes pour la route biochimique de l'histidine en Pichia et leur utilisation
Ward Single-step purification of shuttle vectors from yeast for high frequency back-transformation into E. coli.
CA2168037A1 (fr) Lignee transformee de levure candida utilis et expression d'un heterogene dans cette lignee
Svensson et al. Studies on microbial RNA: I. Transfer of methyl groups from methionine to soluble RNA from Escherichia coli
WO2000056900A3 (fr) Promoteurs exprimant les genes d'une cellule fongique
EP0897010A3 (fr) Phytases modifiées
IE852473L (en) Transformation of yeasts
CA2058633A1 (fr) Methode pour tuer des cellules sans lyse
JPS5592672A (en) Preparation of yeast extract
CA2039021A1 (fr) Sequence d'adn comprenant un codage des genes de structure pour le xylose-reductase ou le xylitol-deshydrogenase
CA2124214A1 (fr) Materiaux et methodes pour la biosynthese de la serine et de produits apparentes a la serine
AU2670688A (en) Co-expression in eukaryotic cells
WO1990009449A3 (fr) Systeme de transformation du candida tropicalis
ATE395424T1 (de) Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
WO1994000572A3 (fr) Gene de l'ataxie-telangiectasies du groupe de complementation d (atdc)
CA2167520A1 (fr) Genes codant un complexe de deshydrogenase et d¨alpha-cetoacide a chaine ramifiee de streptomyces avermitilis
US4081327A (en) Preparation of d-fructose
EP0393969A3 (fr) ADN contenant le gène de l'IMP-déhydrogénase et son utilisation
Macino et al. Assembly of the mitochondrial membrane system: two separate genes coding for threonyl-tRNA in the mitochondrial DNA of Saccharomyces cerevisiae
EP0151341A1 (fr) ADN et son utilisation
NISHIKAWA et al. Structure and Function of 5S Ribosomal Ribonucleic Acid from Torulopsis utilis: I. Purification and Complete Digestion with Pancreatic Ribonuclease A and Ribonuclease T1
CA2044911A1 (fr) Methode de preparation d'acide 2-phosphate ascorbique
EP0979876A3 (fr) Procédé de préparation d'un produit par fermentation et micro-organisme résistant au stress
EP0085958A3 (fr) Procédé de production de L-phénylalanine par fermentation
CA2286395A1 (fr) Proteine heterologue et sa methode de production

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): BR CA HU JP NO

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): BR CA HU JP NO

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 1990906540

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1990906540

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1990906540

Country of ref document: EP