UY36522A - Ultracentrifugación analítica para la caracterización de partículas virales recombinantes - Google Patents
Ultracentrifugación analítica para la caracterización de partículas virales recombinantesInfo
- Publication number
- UY36522A UY36522A UY0001036522A UY36522A UY36522A UY 36522 A UY36522 A UY 36522A UY 0001036522 A UY0001036522 A UY 0001036522A UY 36522 A UY36522 A UY 36522A UY 36522 A UY36522 A UY 36522A
- Authority
- UY
- Uruguay
- Prior art keywords
- recombinant
- recombinant viral
- particles
- viral particles
- analytical ultracentrifugation
- Prior art date
Links
- 239000002245 particle Substances 0.000 title abstract 10
- 230000003612 virological effect Effects 0.000 title abstract 9
- 238000013103 analytical ultracentrifugation Methods 0.000 title abstract 2
- 238000012512 characterization method Methods 0.000 title 1
- 210000000234 capsid Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000002360 preparation method Methods 0.000 abstract 2
- 241000700584 Simplexvirus Species 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
- G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se proporcionan aquí métodos para caracterizar preparaciones de partículas virales recombinantes usando ultracentrifugación analítica. Las partículas virales recombinantes incluyen partículas virales adeno-asociadas recombinantes, partículas adenovirales recombinantes, partículas lentivirales recombinantes y partículas de virus herpes simplex recombinantes. Se pueden identificar y cuantificar especies variantes de partículas virales recombinantes que incluyen cápsides vacías y partículas virales recombinantes con genomas variantes (v.g., genomas truncados, agregados, recombinantes). Los métodos se pueden usar para caracterizar preparaciones de partículas virales recombinantes independientemente de la secuencia del genoma viral recombinante o el serotipo de la cápside viral recombinante.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562105714P | 2015-01-20 | 2015-01-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
UY36522A true UY36522A (es) | 2016-08-31 |
Family
ID=55305080
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UY0001036522A UY36522A (es) | 2015-01-20 | 2016-01-20 | Ultracentrifugación analítica para la caracterización de partículas virales recombinantes |
Country Status (13)
Country | Link |
---|---|
US (4) | US10429288B2 (es) |
EP (1) | EP3247793A1 (es) |
JP (3) | JP2018505695A (es) |
KR (1) | KR20170104594A (es) |
CN (1) | CN107646052A (es) |
AR (1) | AR105412A1 (es) |
AU (1) | AU2016209471B2 (es) |
CA (1) | CA2974378A1 (es) |
EA (1) | EA201791630A1 (es) |
IL (4) | IL296391B2 (es) |
TW (1) | TWI745285B (es) |
UY (1) | UY36522A (es) |
WO (1) | WO2016118520A1 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL296391B2 (en) | 2015-01-20 | 2024-06-01 | Genzyme Corp | Analytical ultracentrifugation for characterization of recombinant viral particles |
EP3696534A1 (en) * | 2019-02-14 | 2020-08-19 | SmartDyeLivery GmbH | Method for determining physicochemical properties of nanoscale systems (nss) |
US20230242939A1 (en) * | 2020-01-29 | 2023-08-03 | Voyager Therapeutics, Inc. | Methods and systems for producing aav particles |
GB202004254D0 (en) | 2020-03-24 | 2020-05-06 | Puridify Ltd | Characterization of gene therapy vectors |
US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
CN117460832A (zh) | 2021-03-22 | 2024-01-26 | 建新公司 | 空aav衣壳和完整aav衣壳的尺寸排阻色谱分析 |
WO2022224697A1 (ja) | 2021-04-19 | 2022-10-27 | 国立大学法人大阪大学 | 分子送達用粒子を特徴づけるための方法 |
TWI787809B (zh) * | 2021-05-07 | 2022-12-21 | 台達電子工業股份有限公司 | 資料預篩方法及其資料預篩裝置 |
WO2024025787A1 (en) * | 2022-07-26 | 2024-02-01 | Brammer Bio, Llc | Analytical ultracentrifugation methods |
WO2024079655A1 (en) | 2022-10-11 | 2024-04-18 | Meiragtx Uk Ii Limited | Chromatography methods for purification of aav capsids |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5622856A (en) * | 1995-08-03 | 1997-04-22 | Avigen | High efficiency helper system for AAV vector production |
US6001650A (en) | 1995-08-03 | 1999-12-14 | Avigen, Inc. | High-efficiency wild-type-free AAV helper functions |
ATE258987T1 (de) | 1995-08-03 | 2004-02-15 | Avigen Inc | Helfersystem zur wirkungssteigerung der aav- vektorproduktion |
US6027931A (en) | 1995-08-03 | 2000-02-22 | Avigen, Inc. | High-efficiency AA V helper functions |
US6593103B1 (en) * | 1997-02-07 | 2003-07-15 | The Regents Of The University Of California | HIV capsid assembly-associated compositions and method |
US6995006B2 (en) | 1997-09-05 | 2006-02-07 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
US6566118B1 (en) | 1997-09-05 | 2003-05-20 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
ES2308989T3 (es) | 1999-08-09 | 2008-12-16 | Targeted Genetics Corporation | Aumento de la expresion de una secuencia nucleotidica heterologa a partir de vectores viricos recombinantes que contienen una secuencia que forman pares de bases intracatenarios. |
CA2385823A1 (en) | 1999-10-01 | 2001-04-12 | Haifeng Chen | Production of recombinant aav using adenovirus comprising aav rep/cap genes |
CA2410828C (en) | 2000-06-01 | 2012-01-24 | University Of North Carolina At Chapel Hill | Duplexed parvovirus vectors |
US6723551B2 (en) | 2001-11-09 | 2004-04-20 | The United States Of America As Represented By The Department Of Health And Human Services | Production of adeno-associated virus in insect cells |
ATE520707T1 (de) | 2001-11-13 | 2011-09-15 | Univ Pennsylvania | Verfahren für den nachweis und/oder die identifikation von sequenzen des adeno- assoziierten virus (aav) sowie isolation von dadurch identifizierten, neuen sequenzen |
SI2277996T1 (sl) | 2003-05-21 | 2014-12-31 | Genzyme Corporation | Postopki za izdelavo pripravkov rekombinantnih AAV virionov v bistvu brez praznih kapsid |
US7765583B2 (en) | 2005-02-28 | 2010-07-27 | France Telecom | System and method for managing virtual user domains |
WO2006119432A2 (en) | 2005-04-29 | 2006-11-09 | The Government Of The U.S.A., As Rep. By The Sec., Dept. Of Health & Human Services | Isolation, cloning and characterization of new adeno-associated virus (aav) serotypes |
WO2007120542A2 (en) | 2006-03-30 | 2007-10-25 | The Board Of Trustees Of The Leland Stanford Junior University | Aav capsid library and aav capsid proteins |
BRPI1015176B1 (pt) | 2009-06-16 | 2019-10-29 | Genzyme Corp | método para isolar uma população de partículas de vírus adenoassociado recombinante (raav) de impurezas de processo em uma corrente de alimentação |
US8663624B2 (en) | 2010-10-06 | 2014-03-04 | The Regents Of The University Of California | Adeno-associated virus virions with variant capsid and methods of use thereof |
PL2675902T3 (pl) | 2011-02-17 | 2019-08-30 | The Trustees Of The University Of Pennsylvania | Kompozycje i sposoby do zmieniania specyficzności względem tkanki i poprawy transferu genu z udziałem aav9 |
FR3002237B1 (fr) | 2013-02-15 | 2017-12-15 | Genethon | Methodes pour la production de particules virales aav double brin |
EP3230441A4 (en) * | 2014-12-12 | 2018-10-03 | Voyager Therapeutics, Inc. | Compositions and methods for the production of scaav |
IL296391B2 (en) | 2015-01-20 | 2024-06-01 | Genzyme Corp | Analytical ultracentrifugation for characterization of recombinant viral particles |
-
2016
- 2016-01-19 IL IL296391A patent/IL296391B2/en unknown
- 2016-01-19 US US15/544,498 patent/US10429288B2/en active Active
- 2016-01-19 EA EA201791630A patent/EA201791630A1/ru unknown
- 2016-01-19 JP JP2017556788A patent/JP2018505695A/ja active Pending
- 2016-01-19 WO PCT/US2016/013947 patent/WO2016118520A1/en active Application Filing
- 2016-01-19 CA CA2974378A patent/CA2974378A1/en active Pending
- 2016-01-19 TW TW105101529A patent/TWI745285B/zh active
- 2016-01-19 IL IL310375A patent/IL310375A/en unknown
- 2016-01-19 CN CN201680016288.9A patent/CN107646052A/zh active Pending
- 2016-01-19 AU AU2016209471A patent/AU2016209471B2/en active Active
- 2016-01-19 EP EP16703205.1A patent/EP3247793A1/en active Pending
- 2016-01-19 KR KR1020177023001A patent/KR20170104594A/ko not_active Application Discontinuation
- 2016-01-20 UY UY0001036522A patent/UY36522A/es not_active Application Discontinuation
- 2016-01-20 AR ARP160100143A patent/AR105412A1/es unknown
-
2017
- 2017-07-18 IL IL253542A patent/IL253542B/en active IP Right Grant
-
2019
- 2019-08-21 US US16/547,144 patent/US11639887B2/en active Active
-
2020
- 2020-04-22 IL IL274137A patent/IL274137B2/en unknown
- 2020-09-11 JP JP2020152446A patent/JP2021000110A/ja active Pending
-
2022
- 2022-04-12 JP JP2022065460A patent/JP2022095863A/ja active Pending
-
2023
- 2023-03-22 US US18/188,176 patent/US20230288308A1/en active Pending
- 2023-11-10 US US18/506,853 patent/US12013326B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN107646052A (zh) | 2018-01-30 |
US20230288308A1 (en) | 2023-09-14 |
US20180180525A1 (en) | 2018-06-28 |
JP2018505695A (ja) | 2018-03-01 |
JP2021000110A (ja) | 2021-01-07 |
IL253542B (en) | 2020-05-31 |
US12013326B2 (en) | 2024-06-18 |
CA2974378A1 (en) | 2016-07-28 |
TWI745285B (zh) | 2021-11-11 |
AR105412A1 (es) | 2017-10-04 |
IL310375A (en) | 2024-03-01 |
US10429288B2 (en) | 2019-10-01 |
US11639887B2 (en) | 2023-05-02 |
JP2022095863A (ja) | 2022-06-28 |
IL296391A (en) | 2022-11-01 |
TW201639958A (zh) | 2016-11-16 |
AU2016209471B2 (en) | 2022-03-31 |
EA201791630A1 (ru) | 2018-04-30 |
KR20170104594A (ko) | 2017-09-15 |
AU2016209471A1 (en) | 2017-09-07 |
US20240085301A1 (en) | 2024-03-14 |
US20240077402A1 (en) | 2024-03-07 |
IL296391B2 (en) | 2024-06-01 |
IL274137A (en) | 2020-06-30 |
IL296391B1 (en) | 2024-02-01 |
EP3247793A1 (en) | 2017-11-29 |
US20200225139A1 (en) | 2020-07-16 |
IL274137B2 (en) | 2023-02-01 |
WO2016118520A1 (en) | 2016-07-28 |
IL274137B (en) | 2022-10-01 |
IL253542A0 (en) | 2017-09-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
109 | Application deemed to be withdrawn |
Effective date: 20221027 |