US20040185062A1 - Canine vaccines against Bordetella bronchiseptica - Google Patents

Canine vaccines against Bordetella bronchiseptica Download PDF

Info

Publication number
US20040185062A1
US20040185062A1 US10/767,809 US76780904A US2004185062A1 US 20040185062 A1 US20040185062 A1 US 20040185062A1 US 76780904 A US76780904 A US 76780904A US 2004185062 A1 US2004185062 A1 US 2004185062A1
Authority
US
United States
Prior art keywords
combination vaccine
leptospira
vaccine
canine
dogs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/767,809
Other languages
English (en)
Inventor
Paul Dominowski
Joseph Frantz
Richard Krebs
Shelly Shields
Robert Sorensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Priority to US10/767,809 priority Critical patent/US20040185062A1/en
Assigned to PFIZER INC. reassignment PFIZER INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FRANTZ, JOSEPH C., SORENSEN, ROBERT GREG, KREBS, RICHARD L., SHIELDS, SHELLY L., DOMINOWSKI, PAUL J.
Publication of US20040185062A1 publication Critical patent/US20040185062A1/en
Priority to US10/959,757 priority patent/US20050089533A1/en
Priority to US11/942,843 priority patent/US7736658B2/en
Priority to US11/962,699 priority patent/US20080175860A1/en
Assigned to PFIZER INC. reassignment PFIZER INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PFIZER PRODUCTS INC.
Assigned to PFIZER INC. reassignment PFIZER INC. CORRECTION BY DECLARATION OF INCORRECT NUMBERS RECORDED AT REEL 029041 FRAME 0099. Assignors: PFIZER INC.
Abandoned legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/0225—Spirochetes, e.g. Treponema, Leptospira, Borrelia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/099—Bordetella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/155—Paramyxoviridae, e.g. parainfluenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/155—Paramyxoviridae, e.g. parainfluenza virus
    • A61K39/175—Canine distemper virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/23—Parvoviridae, e.g. feline panleukopenia virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/235—Adenoviridae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/295—Polyvalent viral antigens; Mixtures of viral and bacterial antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/12—Mucolytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K2039/10—Brucella; Bordetella, e.g. Bordetella pertussis; Not used, see subgroups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/521—Bacterial cells; Fungal cells; Protozoal cells inactivated (killed)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55577—Saponins; Quil A; QS21; ISCOMS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
    • C12N2760/18434—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Definitions

  • This invention relates to vaccines containing a Bordetella bronchiseptica p68 antigen and the use thereof for protecting dogs against infectious tracheobronchitis (“kennel cough”) caused by Bordetella bronchiseptica .
  • This invention also relates to combination vaccines containing a Bordetella bronchiseptica p68 antigen and one or more antigens of another canine pathogen such as canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine coronavirus (CCV), canine parvovirus (CPV), Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona .
  • CD canine distemper
  • CAV-2 canine adenovirus type 2
  • CMV canine parainfluenza
  • CCV canine coronavirus
  • CPV canine parvovirus
  • Leptospira bratislava Leptospira canicola
  • Leptospira grippotyphosa Lepto
  • the present commercially available canine Bordetella bronchiseptica vaccine product is composed of an inactivated, nonadjuvanted Bordetella bronchiseptica whole cell bacterin. Such whole cell bacterin can lead to cell protein related post-vaccination reactions.
  • the p68 protein of B. bronchiseptica is antigenically similar to the Outer Membrane Protein (OMP) of B. pertussis and the OMP of B. parapertussis (Shahin et al., “Characterization of the Protective Capacity and Immunogenicity of the 69-kD Outer Membrane Protein of Bordetella pertussis”, J. Exp. Med., 171: 63-73, 1990).
  • mice Shahin et al., supra; Novotny et al., “Biologic and Protective Properties of the 69-kD Outer Membrane Protein of Bordetella pertussis : A Novel Formulation for a Acellular Pertussis Vaccine”, J. Infect. Dis. 164:114-22, 1991), humans (He et al., “Protective Role of Immunoglobulin G Antibodies to Filamentous Hemagglutinin and Pertactin of Bordetella pertussis in Bordetella parapertussis Infection”, Eur. J. Clin Microbiol Infect Dis.
  • CD is a universal, high-mortality viral disease with variable manifestations. Approximately 50% of nonvaccinated, nonimmune dogs infected with CD virus develop clinical signs, and approximately 90% of those dogs die.
  • Infectious canine hepatitis or ICH caused by canine adenovirus type 1 (CAV-1), is a universal, sometimes fatal, viral disease of dogs characterized by hepatic and generalized endothelial lesions.
  • CAV-2 causes respiratory disease, which, in severe cases, may include pneumonia and bronchopneumonia.
  • CPI is a common viral upper respiratory disease. Uncomplicated CPI may be mild or subclinical, with signs becoming more severe if concurrent infection with other respiratory pathogens exists.
  • CPV infection results in enteric disease characterized by sudden onset of vomiting and diarrhea, often hemorrhagic. Leukopenia commonly accompanies clinical signs. Susceptible dogs of any age can be affected, but mortality is greatest in puppies. In puppies 4-12 weeks of age CPV may occasionally cause myocarditis that can result in acute heart failure after a brief and inconspicuous illness. Following infection many dogs are refractory to the disease for a year or more. Similarly, seropositive bitches may transfer to their puppies CPV antibodies which can interfere with active immunization of the puppies through 16 weeks of age.
  • CCV enteric disease in susceptible dogs of all ages worldwide. Highly contagious, the virus is transmitted primarily through direct contact with infectious feces, and may cause clinical enteritis within 1-4 days after exposure. Severity of disease may be exacerbated by concurrent infection with other agents. Primary signs of CCV infection include anorexia, vomiting, and diarrhea. Frequency of vomiting usually diminishes within a day or 2 after onset of diarrhea, but diarrhea may linger through the course of infection, and stools occasionally may contain streaks of blood. With CCV infection most dogs remain afebrile and leukopenia is not observed in uncomplicated cases.
  • Leptospirosis occurs in dogs of all ages, with a wide range of clinical signs and chronic nephritis generally following acute infection. Infection with L. canicola and L. icterohaemorrhagiae cannot be differentiated clinically.
  • the present invention provides vaccines and methods for protecting dogs against diseases caused by canine pathogens.
  • the present invention provides p68 vaccines suitable for administration to dogs and capable of protecting dogs against disease caused by Bordetella bronchiseptica .
  • Such vaccines of the present invention include a Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant.
  • the present invention provides methods of protecting dogs against disease caused by Bordetella bronchiseptica by administering to a dog a vaccine which includes a Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant.
  • the present invention provides combination vaccines suitable for administration to dogs.
  • the combination vaccines of the present invention include a Bordetella bronchiseptica p68 antigen in combination with at least one other antigen from other canine pathogens, capable of inducing a protective immune response in dogs against disease caused by such other pathogen(s).
  • Such other pathogens can be selected from canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), canine coronavirus (CCV), canine herpesvirus, rabies virus, Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira pomona, Leptospira hardjobovis, Porphyromonas spp., Bacteriodes spp., Leishmania spp., Borrelia spp., Ehrlichia spp., Mycoplasma spp. and Microsporum canis.
  • CD canine distemper
  • CAV-2 canine adenovirus type 2
  • CAV-2 canine parainfluenza
  • CPV canine parvovirus
  • a preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); and a Bordetella bronchiseptica p68 antigen.
  • Another preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); a Bordetella bronchiseptica p68 protein, and an inactivated cell preparation of five Leptospira serovars ( Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona ).
  • Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; and a Bordetella bronchiseptica p68 antigen.
  • Another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; a Bordetella bronchiseptica p68 antigen; and an inactivated cell preparation of Leptospira canicola and Leptospira icterohaemorrhagiae.
  • Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain, a Bordetella bronchiseptica p68 antigen and an inactivated cell preparation of five Leptospira serovars ( Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona ).
  • Another preferred combination vaccine includes a Bordetella bronchiseptica p68 antigen and an attenuated CPI virus.
  • Still another preferred combination vaccine includes a Bordetella bronchiseptica p68 antigen, an attenuated CPI virus and an inactivated cell preparation of Leptospira canicola and Leptospira icterohaemorrhagiae.
  • the present invention also provides methods of protecting dogs against disease caused by a canine pathogen by administering to a dog a combination vaccine of the present invention.
  • FIG. 1 Summary of the geometric mean of p68 ELISA endpoint titers in unvaccinated and Bordetella p68 (15 ⁇ g/dose) vaccinated dogs-aerosol challenge with Bordetella bronchiseptica.
  • FIG. 2 Summary of Serum Amyloid A titers in dogs following aerosol challenge with Bordetella bronchiseptica.
  • FIG. 3 Summary of the geometric mean of p68 ELISA endpoint titers in unvaccinated and Bordetella p68 vaccinated dogs following vaccination and aerosol challenge with Bordetella bronchiseptica.
  • FIG. 4 Summary of Serum Amyloid A titers in dogs following aerosol challenge with Bordetella bronchiseptica.
  • FIG. 5 Western blot showing reactivity of p68 monoclonal antibody Bord 2-7 to p68 whole cell lysate.
  • the present invention provides monovalent vaccines suitable for administration to dogs which are capable of protecting dogs against disease caused by Bordetella bronchiseptica .
  • the monovalent vaccines of the present invention include a recombinantly produced Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant.
  • the present invention provides methods of protecting dogs against disease caused by Bordetella bronchiseptica by administering to a dog a monovalent vaccine which includes a recombinantly produced Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant.
  • the present invention provides combination vaccines suitable for administration to dogs.
  • the combination vaccines of the present invention include a recombinantly produced Bordetella bronchiseptica p68 antigen in combination with at least one other antigen capable of inducing a protective immune response in dogs against disease caused by such other antigen.
  • a preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); and a Bordetella bronchiseptica p68 protein.
  • Another preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); a Bordetella bronchiseptica p68 protein, and a preparation of five Leptospira serovars ( Leptospira Bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona ).
  • Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; and a Bordetella bronchiseptica p68 protein.
  • Another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; a Bordetella bronchiseptica p68 protein; and a preparation of Leptospira canicola and Leptospira icterohaemorrhagiae.
  • Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain and a preparation of five Leptospira serovars ( Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona ).
  • the present invention also provides methods of protecting dogs against disease caused by a canine pathogen by administering to a dog a combination vaccine of the present invention.
  • protecting a dog against a disease caused by a canine pathogen means reducing or eliminating the risk of infection by the pathogen, ameliorating or alleviating the symptoms of an infection, or accelerating the recovery from an infection. Protection is achieved if there is a reduction in viral or bacterial load, a reduction in viral or bacterial shedding, a decrease in incidence or duration of infections, reduced acute phase serum protein levels, reduced rectal temperatures, and/or increase in food uptake and/or growth, for example.
  • p68 antigen refers to a protein with a molecular weight of 68 kDa as determined by SDS polyacrylamide gel electrophoresis, is recognized by the p68-specific monoclonal antibody Bord 2-7 (ATCC#), and has an amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence that is substantially identical to SEQ ID NO: 1.
  • substantially identical is meant a degree of sequence identity of at least about 90%, preferably at least about 95%, or more preferably, at least about 98%.
  • a p68 monovalent vaccine refers to a vaccine having one principal antigenic component.
  • a p68 monovalent vaccine includes a Bordetella bronchiseptica p68 antigen as the principal antigenic component of the vaccine and is capable of protecting the animal to which the vaccine is administered against diseases caused by Bordetella bronchiseptica.
  • combination vaccine is meant a bivalent or multivalent combination of antigens which are capable of inducing a protective immune response in dogs.
  • the protective effects of a combination vaccine against a pathogen or pathogens are normally achieved by inducing in the animal subject an immune response, either a cell-mediated or a humoral immune response or a combination of both.
  • immunogenic is meant the capacity of a composition to provoke an immune response in dogs against a particular pathogen.
  • the immune response can be a cellular immune response mediated primarily by cytotoxic T-cells and cytokine-producing T-cells, or a humoral immune response mediated primarily by helper T-cells, which in turn activates B-cells leading to antibody production.
  • the term “therapeutically effective amount” or “effective amount” refers to an amount of a monovalent or combination vaccine sufficient to elicit a protective immune response in the dog to which it is administered.
  • the immune response may comprise, without limitation, induction of cellular and/or humoral immunity.
  • the amount of a vaccine that is therapeutically effective may vary depending on the particular antigen used in the vaccine, the age and condition of the dog, and/or the degree of infection, and can be determined by a veterinary physician.
  • the present invention has demonstrated for the first time that a vaccine composition containing a Bordetella bronchiseptica p68 antigen effectively protected dogs against disease caused by Bordetella bronchiseptica .
  • the vaccine composition of the present invention does not cause significant post-vaccination reactions, is safe for administration to puppies, and induces protective immunity in dogs that lasts for an extended period of time.
  • one embodiment of the present invention is directed to a vaccine composition containing a Bordetella bronchiseptica p68 antigen (or “a p68 vaccine”), that is suitable for administration to dogs and is capable of protecting dogs against disease caused by Bordetella bronchiseptica , e.g., infectious tracheobronchitis (“kennel cough”).
  • a Bordetella bronchiseptica p68 antigen or “a p68 vaccine”
  • p68 antigen refers to a protein with a molecular weight of 68 kDa as determined by SDS polyacrylamide gel electrophoresis, is recognized by the p68-specific monoclonal antibody Bord 2-7 (ATCC#), and has an amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence that is substantially identical to SEQ ID NO: 1.
  • substantially identical is meant a degree of sequence identity of at least about 90%, preferably at least about 95%, or more preferably, at least about 98%.
  • p68 antigen having an amino acid sequence substantially identical to SEQ ID NO: 1 is the p68 antigen described in WO 92/17587, which is set forth in SEQ ID NO: 3.
  • the p68 specific monoclonal antibody of the present invention recognizes native p68 proteins, recombinant p68 proteins and p68 proteins on the surface of bacteria, for example.
  • p68 antigens suitable for use in the present invention include both native p68 proteins (i.e., naturally occurring p68 proteins purified from Bordetella bronchiseptica ) and recombinantly produced p68 proteins.
  • p68 Purification of native p68 from Bordetella bronchiseptica is described, e.g., in Montaraz et al., Infection and Immunity 47: 744-751 (1985), and is also illustrated in the examples provided hereinbelow. Recombinant production of p68 can be achieved using any one of the molecular cloning and recombinant expression techniques known to those skilled in the art.
  • a nucleic acid molecule encoding p68 can be introduced into an appropriate host cell, such as a bacterium, a yeast cell (e.g., a Pichia cell), an insect cell or a mammalian cell (e.g., CHO cell).
  • the p68-encoding nucleic acid molecule can be placed in an operable linkage to a promoter capable of effecting the expression of the p68 antigen in the host cell.
  • p68 which is expressed by the host cell, can be readily purified using routine protein purification techniques.
  • the nucleotide sequence as set forth in SEQ ID NO: 2 coding for the p68 antigen which has the amino acid sequence of SEQ ID NO: 1 is cloned in an expression vector and placed in an operable linkage to a temperature sensitive promoter.
  • the expression vector is introduced into Escherichia coli and the p68 antigen is expressed upon heat induction.
  • the cells are lysed and the inclusion bodies where the p68 antigen accumulates are separated by centrifugation.
  • the recombinant p68 in the inclusion bodies is solubilized using SDS or other solubilization agents known in the art such as urea, guanidine hydrochloride, sodium cholate, taurocholate, and sodium deoxycholate.
  • SDS solubilization agents known in the art
  • a purified native or recombinant p68 protein is combined with a veterinary-acceptable carrier to form a p68 vaccine composition.
  • a veterinary-acceptable carrier includes any and all solvents, dispersion media, coatings, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, adsorption delaying agents, and the like.
  • Diluents can include water, saline, dextrose, ethanol, glycerol, and the like.
  • Isotonic agents can include sodium chloride, dextrose, mannitol, sorbitol, and lactose, among others.
  • Stabilizers include albumin, among others.
  • Adjuvants suitable for use in accordance with the present invention include, but are not limited to several adjuvant classes such as; mineral salts, e.g., Alum, aluminum hydroxide, aluminum phosphate and calcium phosphate; surface-active agents and microparticles, e.g., nonionic block polymer surfactants (e.g., cholesterol), virosomes, saponins (e.g., Quil A, QS-21 and GPI-0100), proteosomes, immune stimulating complexes, cochleates, quarterinary amines (dimethyl diocatadecyl ammonium bromide (DDA)), pyridine, vitamin A, vitamin E; bacterial products such as the RIBI adjuvant system (Ribi Inc.), cell wall skeleton of Mycobacterum phlei (Detox®), muramyl dipeptides (MDP) and tripeptides (MTP), monophosphoryl lipid A, Bacillus Calmete-Guerin,
  • coli enterotoxins cholera toxin, trehalose dimycolate, CpG oligodeoxnucleotides
  • cytokines and hormones e.g., interleukins (IL-1, IL-2, IL-6, IL-12, IL-15, IL-18), granulocyte-macrophage colony stimulating factor, dehydroepiandrosterone, 1,25-dihydroxy vitamin D3
  • polyanions e.g., dextran
  • polyacrylics e.g., polymethylmethacrylate, Carbopol 934P
  • carriers e.g., tetanus toxid, diptheria toxoid, cholera toxin B subnuit, mutant heat labile enterotoxin of enterotoxigenic E.
  • rmLT heat shock proteins
  • oil-in-water emulsions e.g., AMPHIGEN® (Hydronics, USA)
  • water-in-oil emulsions such as, e.g., Freund's complete and incomplete adjuvants.
  • Preferred adjuvants for use in the vaccines of the present invention include Quil A and cholesterol.
  • the p68 antigen and the veterinary-acceptable carrier can be combined in any convenient and practical manner to form a vaccine composition, e.g., by admixture, solution, suspension, emulsification, encapsulation, absorption and the like, and can be made in formulations such as tablets, capsules, powder, syrup, suspensions that are suitable for injections, implantations, inhalations, ingestions or the like.
  • the vaccine is formulated such that it can be administered to dogs by injection in a dose of about 0.1 to 5 ml, or preferably about 0.5 to 2.5 ml, or even more preferably, in a dose of about 1 ml.
  • the pharmaceutical compositions of the present invention should be made sterile by well-known procedures.
  • the amount of p68 in the vaccines should be immunizing-effective and is generally in the range of 0.5-1000 ⁇ g per dose.
  • the amount of p68 is in the range of 1-260 ⁇ g per dose. More preferably, the amount of p68 is in the range of 10-100 ⁇ g per dose. Even more preferably, the amount of p68 is about 15 to 25 ⁇ g per dose.
  • the amount of adjuvants suitable for use in the vaccines depends upon the nature of the adjuvant used.
  • Quil A and cholesterol are used as adjuvant
  • Quil A is generally in an amount of about 1-1000 ⁇ g per dose, preferably 30-100 ⁇ g per dose, and more preferably, about 50-75 ⁇ g per dose
  • cholesterol is generally in an amount of about 1-1000 ⁇ g per dose, preferably about 30-100 ⁇ g per dose, and more preferably, about 50-75 ⁇ g per dose.
  • the present invention provides methods of protecting dogs against disease caused by Bordetella bronchiseptica by administering to a dog a p68 vaccine composition, as described hereinabove.
  • the p68 vaccine composition provides dogs with a long term immunity for at least about 4 months, preferably for at least about 6 months, or even more preferably, for about one year,
  • a p68 vaccine can be administered to a dog by any known routes, including the oral, intranasal, mucosal, topical, transdermal, and parenteral (e.g., intravenous, intraperitoneal, intradermal, subcutaneous or intramuscular). Administration can also be achieved using needle-free delivery devices. Administration can be achieved using a combination of routes, e.g., first administration using a parental route and subsequent administration using a mucosal route.
  • Preferred routes of administration include subcutaneous and intramuscular administrations.
  • the p68 vaccine composition of the present invention can be administered to dogs of at least 6 weeks old, preferably at least 7 weeks old, and more preferably, at least 8 or 9 weeks old. Dogs can be vaccinated with one dose or with more than one dose of a p68 vaccine. Preferably, two doses of a p68 vaccine are administered to dogs with an interval of about 2-4 weeks, preferably about 3 weeks, between the two administrations. If dogs are vaccinated before the age of 4 months, it is recommended that they be revaccinated with a single dose upon reaching 4 months of age, because maternal antibodies may interfere with development of an adequate immune response in puppies less than 4 months old. Dogs can also be revaccinated annually with a single dose. Where B. bronchiseptica exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 1 year, or preferably 6 months, of the occurrence of these events.
  • the present invention provides combination vaccines and methods for protecting dogs against Bordetella bronchiseptica and one or more other canine pathogens by administering such combination vaccines.
  • the combination vaccine compositions of the present invention do not exhibit efficacy interference and are safe for administration to puppies.
  • the combination vaccines of the present invention include a Bordetella bronchiseptica p68 antigen, which can be made as described hereinabove, in combination with at least one antigen from other canine pathogens capable of inducing a protective immune response in dogs against disease caused by such other pathogens.
  • Such other pathogens include, but are not limited to, canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), canine coronavirus (CCV), canine herpesvirus, and rabies virus.
  • Antigens from these pathogens for use in the vaccine compositions of the present invention can be in the form of a modified live viral preparation or an inactivated viral preparation. Methods of attenuating virulent strains of these viruses and methods of making an inactivated viral preparation are known in the art and are described in, e.g., U.S. Pat. Nos. 4,567,042 and 4,567,043.
  • pathogens also include Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira pomona, Leptospira hardjobovis, Porphyromonas spp., Bacteriodes spp., Leishmania spp., Borrelia spp., Ehrlichia spp., Mycoplasma ssp. and Microsporum canis .
  • Antigens from these pathogens for use in the vaccine compositions of the present invention can be in the form of an inactivated whole or partial cell preparation, using methods well-known in the art.
  • the combination vaccines generally include a veterinary-acceptable carrier.
  • a veterinary-acceptable carrier includes any and all solvents, dispersion media, coatings, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, adsorption delaying agents, and the like.
  • Diluents can include water, saline, dextrose, ethanol, glycerol, and the like.
  • Isotonic agents can include sodium chloride, dextrose, mannitol, sorbitol, and lactose, among others.
  • Stabilizers include albumin, among others.
  • Adjuvants suitable for use in accordance with the present invention include, but are not limited to several adjuvant classes such as; mineral salts, e.g., Alum, aluminum hydroxide, aluminum phosphate and calcium phosphate; surface-active agents and microparticles, e.g., nonionic block polymer surfactants (e.g., cholesterol), virosomes, saponins (e.g., Quil A, QS-21 and GPI-0100), proteosomes, immune stimulating complexes, cochleates, quarterinary amines (dimethyl diocatadecyl ammonium bromide (DDA)), pyridine, vitamin A, vitamin E; bacterial products such as the RIBI adjuvant system (Ribi Inc.), cell wall skeleton of Mycobacterum phlei (Detox®), muramyl dipeptides (MDP) and tripeptides (MTP), monophosphoryl lipid A, Bacillus Calmete-Guerin,
  • coli enterotoxins cholera toxin, trehalose dimycolate, CpG oligodeoxnucleotides
  • cytokines and hormones e.g., interleukins (IL-1, IL-2, IL-6, IL-12, IL-15, IL-18), granulocyte-macrophage colony stimulating factor, dehydroepiandrosterone, 1,25-dihydroxy vitamin D 3
  • polyanions e.g., dextran
  • polyacrylics e.g., polymethylmethacrylate, Carbopol 934P
  • carriers e.g., tetanus toxid, diptheria toxoid, cholera toxin B subnuit, mutant heat labile enterotoxin of enterotoxigenic E.
  • rmLT heat shock proteins
  • oil-in-water emulsions e.g., AMPHIGEN® (Hydronics, USA)
  • water-in-oil emulsions such as, e.g., Freund's complete and incomplete adjuvants.
  • Preferred adjuvants for use in the combination vaccines in accordance with the present invention include Quil A and cholesterol.
  • the p68 antigen, one or more antigens from other pathogens, and the veterinary-acceptable carrier can be combined in any convenient and practical manner to form a combination vaccine composition, e.g., by admixture, solution, suspension, emulsification, encapsulation, absorption and the like, and can be made in formulations such as tablets, capsules, powder, syrup, suspensions that are suitable for injections, implantations, inhalations, ingestions or the like.
  • the vaccine is formulated such that it can be administered to dogs by injection in a dose of about 0.1 to 5 ml, or preferably about 0.5 to 2.5 ml, or even more preferably, in a dose of about 1 ml.
  • p68 combination vaccines can be administered to a dog of at least 6 weeks old, preferably at least 7 weeks old, and more preferably, at least 8 or 9 weeks old.
  • the administration can be done by any known routes, including the oral, intranasal, mucosal topical, transdermal, and parenteral (e.g., intravenous, intraperitoneal, intradermal, subcutaneous or intramuscular). Administration can also be achieved using needle-free delivery devices. Administration can also be achieved using a combination of routes, e.g., first administration using a parental route and subsequent administration using a mucosal route. Preferred routes of administration include subcutaneous and intramuscular administrations.
  • a preferred combination vaccine of the present invention includes an attenuated strain of CD virus, an attenuated strain of CAV-2, an attenuated strain of CPI virus, an attenuated strain of CPV, an inactivated preparation of a strain of CCV, and a Bordetella bronchiseptica p68antigen.
  • An especially preferred combination vaccine includes the attenuated CD virus strain designated as the “Snyder Hill” strain (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CAV-2 strain designated as the “Manhattan” strain (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CPI virus strain having the designation of “NL-CPI-5” (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CPV strain having the designation of “NL-35-D” (National Veterinary Service Laboratory, Ames, Iowa), an inactivated preparation of the CCV strain having the designation of “NL-18” (National Veterinary Service Laboratory, Ames, Iowa), and the recombinant Bordetella bronchiseptica p68 antigen having the sequence of SEQ ID NO: 1.
  • Such combination vaccine also referred to herein as “the p68/5CV combination vaccine” is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains and viral preparation with a liquid preparation, which liquid preparation is composed of the p68 antigen dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol.
  • Leptospira bratislava e.g., a Leptospira bratislava strain which can be obtained from National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira canicola e.g., strain C-5, National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira grippotyphosa e.g., strain MAL 1540, National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira icterohaemorrhagiae e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira pomona e.g., strain T262, National Veterinary Service Laboratory, Ames, Iowa
  • Such combination vaccine also referred to herein as “the p68/5CV-Leptospira combination vaccine” is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and viral preparation with a liquid preparation, which liquid preparation is composed of the p68 antigen and Leptospiral antigens, dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol.
  • the p68/5CV and p68/5CV-Leptospira combination vaccines can be administered to healthy dogs 4 weeks of age or older, preferably 6 weeks or older, and preferably in 3 doses, each administered about 3 weeks apart. Dogs can be revaccinated annually with a single dose. Where B. bronchiseptica and canine virus exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 1 year, or preferably 6 months, of the occurrence of these events.
  • Still another preferred combination vaccine of the present invention includes an attenuated strain of CD virus, an attenuated strain of CAV-2, an attenuated strain of CPI virus, an attenuated strain of CPV, and a recombinant Bordetella bronchiseptica p68 antigen.
  • An especially preferred combination vaccine includes the attenuated CD virus strain designated as the “Synder Hill” strain (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CAV-2 strain designated as the “Manhattan” strain (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CPI virus strain having the designation of “NL-CPI-5” (National Veterinary Service Laboratory, Ames, Iowa), the attenuated CPV strain designated as “NL-35-D” (National Veterinary Service Laboratory, Ames, Iowa), and the recombinant Bordetella bronchiseptica p68 antigen having the sequence of SEQ ID NO: 1.
  • Such combination vaccine also referred to herein as “the p68/DA 2 PP combination vaccine” is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) with a liquid preparation, which liquid preparation is composed of the p68 antigen dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol.
  • Another especially preferred combination vaccine includes the antigenic components of the p68/DA 2 PP combination vaccine as well as inactivated whole cell preparations of two Leptospira species: Leptospira canicola (e.g., strain C-51, National Veterinary Service Laboratory, Ames, Iowa), and Leptospira icterohaemorrhagiae (e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, Iowa).
  • Leptospira canicola e.g., strain C-51, National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira icterohaemorrhagiae e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, Iowa.
  • a preferred combination vaccine can include the antigenic components of the p68/DA 2 PP combination vaccine as well as inactivated whole cell preparations of five Leptospira species: Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona .
  • combination vaccines also referred to herein as “the p68/DA 2 PP-Leptospira combination vaccines”
  • the p68/DA 2 PP and p68/DA 2 PP-Leptospira combination vaccines can be administered to healthy dogs 6 weeks or older, or preferably 8 weeks of age or older, and preferably in 2 doses, each administered about 3 weeks apart.
  • a single dose may be sufficient if given to a dog at least 12 weeks of age. Dogs can be revaccinated annually with a single dose.
  • B. bronchiseptica and canine virus exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 1 year, or preferably 6 months, of the occurrence of these events.
  • Another preferred combination vaccine include a p68 antigen, preferably the recombinant p68 antigen having SEQ ID NO: 1, in combination with an attenuated strain of CPI.
  • Still another preferred combination vaccine include a p68 antigen, preferably the recombinant p68 antigen having SEQ ID NO: 1, an attenuated strain of CPI, and two at least two Leptospira species such as Leptospira canicola (e.g., strain C-51, National Veterinary Service Laboratory, Ames, Iowa), and Leptospira icterohaemorrhagiae (e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, Iowa).
  • Leptospira canicola e.g., strain C-51, National Veterinary Service Laboratory, Ames, Iowa
  • Leptospira icterohaemorrhagiae e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, Iowa.
  • the amount of the p68 antigen and the antigen(s) from one or more other pathogens in the combination vaccines of the present invention should be immunizing-effective.
  • the p68 antigen in a combination vaccine should be in an amount of at least about 0.5 ⁇ g per dose.
  • the attenuated CD virus should be in an amount of at least about 10 2 to about 10 9 TCID50 per dose TCID 50 (tissue culture infectious dose 50% cytopathic effect) per dose, and preferably in the range of about 10 4 to about 10 6 TCID 50 per dose.
  • the attenuated CAV-2 should be in an amount of at least about 10 2 TCID 50 to about 10 9 TCID 50 per dose, preferably in the range of 10 4.0 to about 10 6.0 TCID 50 per dose.
  • the attenuated CPI virus should be in an amount of at least about 10 2 TCID 50 to about 10 9 TCID 50 per dose, and preferably in the range of 10 6 to about 10 8 TCID 50 per dose.
  • the attenuated CPV should be in an amount of at least about 102 TCID 50 to about 10 9 TCID 50 per dose, preferably, an amount in the range of 10 7 to about 10 9 TCID 50 per dose.
  • the amount of CCV in an inactivated viral preparation should be at least about 100 relative units per dose, and preferably in the range of 1000-4500 relative units per dose.
  • Each Leptospira species in the vaccine should be in the range of about 100-3500 NU (nephelometric units) per vaccine dose, and preferably in the range of 200-2000 NU per dose.
  • the combination vaccines are formulated such that the vaccines can be administered to dogs by injection in a dose of 0.1 ml to 5 ml, preferably from 0.5 ml to 2.5 ml, and more preferably, about 1 ml.
  • the experimental vaccine antigen was a recombinant p68 outer membrane protein (SEQ ID NO: 1) of B. bronchiseptica produced by E. coli strain LW68.
  • the vaccine contained varying levels of SDS (sodium dodecyl sulfate) solubilized p68, adjuvanted with 50 ⁇ g of QAC (Quil A/50 ⁇ g cholesterol) in a 1 mL dose.
  • Animals were vaccinated on Day 0 with either the placebo or the experimental vaccine.
  • a second vaccination was administered on Day 21.
  • the first vaccination was administered subcutaneously in the right neck and the second vaccination was administered subcutaneously in the left neck.
  • Rectal temperatures were recorded on the day of vaccination and for three days following each vaccination (Days 0 through 3 and 21 through 24).
  • Blood was collected on the days of vaccination (Days 0 and 21) and on Days 42, 50, and 63 and assayed by ELISA for specific antibodies against the p68 protein purified from B. bronchispetica . Blood was also collected on Days 42, 49, 50, 52, 54, 56 and 58 and analyzed for Serum Amyloid A (SM).
  • SM Serum Amyloid A
  • Purified native p68 was diluted to 600 ng/mL in 0.01 M Borate Buffer and was added to each well at 100 ⁇ L/well. The plates were incubated overnight at 4° C. The plates were then washed once with excess PBS-Tween 20.1% nonfat dried milk in PBS was added to the plates at 200 ⁇ L/well. The plates were then incubated for 1 hour at 37° C. The plates were then washed once with excess PBS-Tween 20.
  • Dog or mouse serum was added at a 1:50 dilution to the top row of the ELISA plates and two fold serially diluted serum was added all the way down the plate. The plates were incubated for 1 hour at 37° C. Subsequently, the plates were washed 3 times with excess PBS-Tween 20.
  • ABTS substrate was added at 100 ⁇ L/well. Approximately 20 minutes later, the plates were read with a Molecular Devices or an equivalent plate reader at 405-490 nm.
  • ELISA titers were log transformed prior to analysis using a general linear mixed model. The 95% level of confidence was used to assess treatment differences. Challenge observations were monitored twice daily for 30 minutes each.
  • Injection site reactions following the first vaccination are presented in Table 1. The largest injection site reactions were observed in T05 (64 ⁇ g) vaccinated animals, with the largest mean injection site reaction measuring only 14.69 cm 3 (two days post vaccination). T03 (4 ⁇ g), T04 (16 ⁇ g) and T06 (256 ⁇ g) vaccinated animals demonstrated varying injection site reactions up to 7 days post vaccination. T02 (1 ⁇ g) vaccinated animals only demonstrated reactions on Day 1 post vaccination. By the seventh day post vaccination, there was no statistically significant difference in injection site reactions among the treatment groups. By Day 14, all injection site reactions had dissipated.
  • Injection site reactions following the second vaccination are presented in Table 2. Following the second vaccination the largest mean injection site reactions were observed in T06 (256 ⁇ g), with the largest mean injection site reaction measuring 50.03 cm 3 (one day post vaccination). Injection site reactions were demonstrated in T05 (64 ⁇ g) and T04 (16 ⁇ g) animals up to 7 days post second vaccination. Minimal injection site reactions were demonstrated in T03 (4 ⁇ g) and T02 (1 ⁇ g) animals up to 7 days post vaccination. Injection site reactions that were not statistically different from the placebo group were demonstrated in T02 (1 ⁇ g) and T03 (4 ⁇ g) post vaccination. Fourteen days post second vaccination no injection site reactions were observed.
  • Incidence and duration of injection site reactions following vaccination are summarized in Table 5.
  • the incidence (or the number of dogs showing a reaction at any time) of a measurable injection site reaction was 100% for T03, T04, T05 and T06 (41 g, 16 ⁇ g, 64 ⁇ g, and 256 ⁇ g, respectively) following the first and second vaccination.
  • Animals that received T02 (1 ⁇ g) demonstrated the least incidence of injection site reactions post vaccination (57.1%).
  • SAA titers are summarized in Table 8. Prior to challenge, geometric mean SAA titers were low in all the treatment groups (range 0.1 to 0.5). Post challenge, T01 GMT titers ranged from 1.5 to 146.0, where p68 treatment groups ranged from 0.3 to 23.1. All treatment groups were statistically different than the placebo on Days 50, 52, 54, and 56. No statistical differences were demonstrated among the p68 vaccines with the exception of T02 (1 ⁇ g) on Day 52 when it demonstrated a statistically different geometric mean from all other p68 treatment groups.
  • T01 placebo
  • T04 (16 ⁇ g) and T05 (64 ⁇ g) vaccinated dogs demonstrated an Incidence of Disease of 55.6% and 66.7%, respectively.
  • T02 Only 28.6% of T02 (1 ⁇ g), 50% of T03 (4 ⁇ g), and 33.3% of T06 (256 ⁇ g) vaccinated dogs were observed coughing for two consecutive days.
  • the objective was to establish a relationship between antigen dose, immune response, and protection in dogs.
  • the p68 antigen doses examined were 1 ⁇ g, 4 ⁇ g, 16 ⁇ g, 64 ⁇ g, and 256 ⁇ g.
  • the p68 antigen doses examined were 1 ⁇ g, 4 ⁇ g, 16 ⁇ g, 64 ⁇ g, and 256 ⁇ g.
  • Sterile saline was used as a placebo vaccine in treatment groups T01 and T02.
  • Canine recombinant p68 Bordetella Bronchiseptica Vaccine was used in treatment groups T03 and T04.
  • the structural gene of the p68 antigen was cloned in Escherichia coli and expression of the gene was regulated by a temperature sensitive promoter.
  • the cells were lysed and the inclusion bodies were separated by centrifugation.
  • the recombinant p68 in the inclusion bodies was solubilized by SDS treatment.
  • the recombinant p68 (15 ⁇ g per mL) was combined with 50 ⁇ g of Quil A and 50 ⁇ g of cholesterol per mL in sterile saline as the diluent. Each one mL dose contained 0.28% of ethanol and 0.01% thimerosal.
  • Bordetella bronchiseptica Bihr Cat strain was prepared as the challenge inoculum using the method currently employed by Biologics Control Laboratories-Microbiology. Bordet-Genou agar plates were plated with a confluent growth of Bordetella bronchiseptica —Bihr Cat strain and incubated for 48 hours at 37.5+/ ⁇ 2.5° C. Virulent phase I colonies were selected and streaked on Bordet-Genou agar and incubated for 24 hours at 37.5+/ ⁇ 2.5° C. After incubation, Bordetella saline was used to wash colonies from the agar and the antigen was diluted to an optical density of 0.80 at 600 nm.
  • a cell count was performed pre- and post-challenge for confirmation of the nephelometer reading.
  • Challenge target concentration was approximately 1 ⁇ 10 9 CFU.
  • the pre-challenge concentration was 2.37 ⁇ 10 9 CFU (100% Phase I) and post-challenge concentration count was 1.35 ⁇ 10 9 CFU (100% Phase I).
  • mice were assigned to treatments according to a generalized block design. Treatments were randomly assigned to rooms. On the day of challenge, animals were randomly assigned to challenge rooms by block.
  • Post vaccination response variables consisted of injection site data, rectal temperatures and p68 ELISA titers. Injection site data was summarized in the following ways: 1) number of animals having a measurable reaction by treatment and day of study, 2) number of animal time points having a measurable reaction by treatment, 3) number of animals having a measurable reaction at any time point by treatment.
  • a priori linear contrasts of the treatment by observation time-point least squares mean were constructed to test treatment group differences at each observation time-point and to compare time-points within each treatment. The 5% level of significance was used for all comparisons.
  • Post challenge response variables consisted of daily coughing observations, p68 ELISA titers and serum amyloid A titers. Number of days coughing during the post challenge period was analyzed using a general linear mixed model.
  • a priori contrasts of the treatment least squares mean was constructed to test treatment group differences. The 5% level of significance was used for all comparisons.
  • a priori linear contrasts of the treatment by observation time-point least squares mean was constructed to test treatment group differences at each observation time-point and to compare time-points within each treatment. The 5% level of significance was used for all comparisons.
  • Day 0 was designated as the day of first vaccination. Vaccinations were administered on Day 0 and repeated 21 days later. For the first vaccination, the right side of the neck was used and for the second vaccination, the left side of the neck was used. Intramuscular injections were administered in the right and left semimembranosus muscle for the first and second vaccinations, respectively. All injection sites were measured three dimensionally for seven days following each vaccination with a follow-up measurement conducted 14 days following vaccination. Rectal temperatures were monitored on the day of vaccination (prior to vaccination) and for three days following each vaccination.
  • Blood for agglutination titers was collected prior to first vaccination and prior to challenge.
  • Blood for anti-p68 ELISA evaluation was collected prior to vaccination on Days 0 and 21 and on Days 35, 45, and 59.
  • Blood for Serum Amyloid A (SAA) assay was collected on the day of challenge (Day 45) and on Days 46, 48, 50, 52 and 54.
  • SAA Serum Amyloid A
  • Tracheal swabs were evaluated for the presence of B. bronchiseptica by culture. Each tracheal swab was streaked onto a Bordetella Selective Agar plate. Positive and negative controls were included. The plates were incubated at 37.5 ⁇ 2.5° C. for 48 ⁇ 4 hours. The resulting colonies on each plate were compared to the positive control and any colony which appeared identical to the positive control was further tested to confirm the presence of B. bronchiseptica . Confirmational testing included the use of TSI, Citrate and Urea Agar and Nitrate Red media.
  • Sera were evaluated for agglutination titers, p68 ELISA analysis or SAA analysis using the following methods:
  • Agglutination titers were serially diluted in microtiter plates using Bordetella saline. Positive and negative controls were included on each plate.
  • B. bronchiseptica Strain 87 grown on Bordet Genou agar, harvested, inactivated and diluted to 20% T at 630 nm was used as the agglutinating antigen and was added to each well. Plates were shaken and incubated at 35 ⁇ 2° C. for 2 hours. Plates were read after a second incubation at room temperature for 22 hours. The endpoint titer was determined using the last well to show 50% agglutination.
  • p68 ELISA titers The recombinant p68 antigen was captured on a 96 well microtiter plate coated with a polyclonal antiserum specific to the Bordetella p68 antigen. Serial two-fold dilutions of the canine serum were added to the plate and incubated. Positive and negative controls at a 1:1000 dilution were included on each plate. A peroxidase labeled affinity purified goat anti-dog IgG indicator conjugate was used to detect antibodies specific for the p68 antigen. A chromogenic substrate ABTS was then added and the plate read when the positive control wells had an O.D. of 1.2+0.2. The titer of a given sample was calculated as the reciprocal of the last dilution with an optical density greater than the mean of the negative control serum dilution plus five standard deviations.
  • SAA titers The canine Serum Amyloid A titers were evaluated using a kit purchased from Accuplex Co., University of Kansas Medical Center, Omaha, Nebr. 68198. Briefly, canine SAA was captured on a microtiter plate coated with a monoclonal anti-canine SAA antibody. Diluted samples of the canine serum were added to the plate followed by a biotin labeled anti-canine antibody conjugate. Following incubation, a peroxidase conjugated streptavidin chromogenic substrate was added. The plate was read after 30 minutes.
  • Injection site reactions are summarized in Tables 12 and 13. Due to technical oversight, no injection site observations were conducted at the 14-day observation following the second vaccination (Day 35).
  • p68 ELISA data are summarized in Table 15 and FIG. 1. Due to the considerable titer response to p68 in the vaccinated dogs, various titration minimums were used at different time-points in the study. Titrations for Days 0 and 21 were started at 50. For Days 35, 45 and 59, titrations were begun at 200. Any value reported as “less than” was divided by 2 prior to analysis. The incremental rise observed in p68 ELISA values for control groups (T01 and T02) during the course of the study is due to these minimum titration values. Agglutination titers remained ⁇ 4.
  • All p68 vaccinated animals demonstrated at least a four-fold increase in titers from the first day of vaccination to the day of challenge (Day 0 vs. Day 45) when compared to placebo vaccinated animals.
  • TABLE 15 Geometric mean and standard errors of p68 (15 ⁇ g/dose) ELISA endpoint titers a in dogs following saline or p68 (15 ⁇ g/dose) vaccination and following B. bronchiseptica aerosol challenge.
  • SM values were determined on Days 0, 1, 3, 5, 7 and 9 following challenge. Serum Amyloid A values are presented in Table 17 and represented in FIG. 2. TABLE 17 Geometric mean and standard errors of Serum Amyloid A titers in saline and p68 (15 ⁇ g/dose) Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica Geometric Mean and Standard Errors of Serum Amyloid A Day of Study a 45 46 48 50 52 54 Std. Std. Std. Std. Std. Std.
  • Aerosol challenge for all treatment groups occurred 24 days following the second vaccination (Day 45). Coughing observations were examined using two methods—disease status based on two consecutive days coughing (presented in Table 19) and percentage of days coughing (presented in Tables 20 and 21). When dogs were evaluated using criteria of two consecutive days coughing, 80% of the p68 vaccinated dogs (SC and IM) coughed at least two consecutive days whereas the Saline SC and Saline IM vaccinated dogs coughed 100% and 87.5%, respectively. When dogs were evaluated using percentage of days observed coughing, p68 SC and IM vaccinated dogs coughed 38.72% and 41.05% of the days observed, respectively.
  • a MLV parvovirus vaccine was administered to dogs upon arrival at the study site. To be eligible for the study, animals were determined to be negative to B. bronchiseptica by tracheal swab and agglutination titer. No vaccines, other than the experimental products, were administered during the study.
  • Dogs were kept in an isolation facility necessary to prevent exposure to B. bronchiseptica and canine pathogens prior to challenge. After aerosol challenge with B. bronchiseptica , isolation procedures were continued to prevent exposure to other canine pathogens.
  • Sterile saline was used as a placebo vaccine in treatment groups T01 and T02.
  • Canine recombinant p68 Bordetella Bronchiseptica Vaccine was used in treatment groups T03 and T04.
  • the structural gene of the p68 antigen was cloned in Escherichia coli and expression of the gene was regulated by a temperature sensitive promoter.
  • the cells were lysed and the inclusion bodies were separated by centrifugation.
  • the recombinant p68 in the inclusion bodies was solubilized by SDS treatment.
  • the 15 ⁇ g p68 and 60 ⁇ g p68 were combined with 50 ⁇ g of Quil A and 50 ⁇ g of cholesterol per mL in sterile Lepto saline as the diluent.
  • the combined components were mixed at 4° C. for 24 hours and passed three times through a microfluidizer. Each one mL dose contained 2.7 ⁇ l of ethanol and 0.0001% thimerosal.
  • p68 concentrations in the experimental vaccines were measured by p68 ELISA. All assays were done in replicates of five (5). All vaccines were used within 6 months of assembly.
  • Bordet-Genou agar plates were plated with Bordetella bronchiseptica —Bihr Cat strain and incubated for 48 hours at 37.5+/ ⁇ 2.5° C. Virulent phase I colonies were selected and streaked on Bordet-Genou agar and incubated for 24 hours at 37.5+/ ⁇ 2.5° C. After incubation, Bordetella saline was used to wash colonies from agar and the cells diluted to an optical density of 0.80 at 600 nm. A cell count was performed pre and post challenge for confirmation of the nephelometer reading. Challenge target concentration was approximately 1 ⁇ 10 9 CFU.
  • the prechallenge concentration count was 1.94 ⁇ 10 9 and the post challenge concentration count was 1.43 ⁇ 10 9 .
  • the prechallenge concentration count was 2.55 ⁇ 10 9 and the post challenge concentration count was 2.13 ⁇ 10 9 .
  • Post vaccination response variables consisted of injection site data, rectal temperatures and p68 ELISA titers.
  • Injection site data was summarized as follows: 1) number of animals having a measurable reaction by treatment and day of study, 2) number of animal time points having a measurable reaction by treatment, 3) number of animals having a measurable reaction at any time point by treatment, 4) duration of a measurable reaction for each animal.
  • a priori linear contrasts of the treatment by observation time point least squares mean was constructed to test treatment group differences at each observation time point and to compare time points within each treatment.
  • the specific comparisons of interest were T01 vs. T03, T01 vs. T05, T03 vs. T05, T02 vs. T04, T02 vs. T06, and T04 vs. T06. If the time point-by-treatment-by-study group interaction term was significant at P ⁇ 0.05, contrasts among treatment groups at each time point and among time points within treatment groups was within each study group, otherwise these contrasts were based on the time point-by-treatment interaction effect least squares mean. The 5% level of significance was used for all comparisons.
  • Post challenge response variables consisted of p68 ELISA titers, Serum Amyloid A titers and daily coughing observations. Post challenge p68 ELISA titers were analyzed as previously described. For Serum Amyloid A (SAA) titer data post challenge, the natural log transformation was applied to titer values prior to analysis using a general linear mixed model.
  • SAA Serum Amyloid A
  • a priori linear contrasts of the treatment least squares mean was constructed to test treatment group differences.
  • the specific comparisons of interest were T01 vs. T03, T01 vs. T05, T03 vs. T05, T02 vs. T04, T02 vs. T06, and T04 vs. T06. If the treatment-by-study group interaction term was significant at P ⁇ 0.05, contrasts among treatment groups was within each study group otherwise contrasts among treatment groups were based on the treatment main effect least squares mean. The 5% level of significance was used for all comparisons.
  • Post challenge coughing observations were amended prior to challenge to comply with USDA recommendations. After challenge, each group of dogs was observed between the third and tenth day following challenge, for a total of 8 days. Animals were observed twice daily for coughing for approximately 45 minutes at each observation period. The interval between observation periods was approximately 12 hours. Personnel unaware of the assigned treatment groups recorded coughing observations.
  • Blood for anti-p68 ELISA evaluation was collected the day before vaccination #1 and #2, on Day 50 and at approximately 30 day intervals thereafter for each group. Blood was also collected the day of challenge and on the final day of post challenge observation.
  • SAA Serum Amyloid A
  • Tracheal swabs were evaluated for the presence of B. bronchiseptica by culture. Each tracheal swab was streaked onto a Bordetella Selective Agar plate. Positive and negative controls are included. The plates were incubated at 37.5 ⁇ 2.5° C. for 48 ⁇ 4 hours. The resulting colonies on each plate were compared to the positive control and any colony which appeared identical to the positive control was further tested to confirm the presence of B. bronchiseptica . Confirmational testing included the use of TSI, Citrate and Urea Agar and Nitrate Red media.
  • Sera were evaluated for agglutination titers, p68 ELISA analysis or SM analysis using the following methods:
  • Agglutination titers were serially diluted in microtiter plates using Bordetella saline. Positive and negative controls were included on each plate.
  • B. bronchiseptica Strain 87 grown on Bordet Genou agar, harvested, inactivated and diluted to 20% T at 630 nm was used as the agglutinating antigen and was added to each well. Plates were shaken and incubated at 35 ⁇ 2° C. for 2 hours. Plates were read after a second incubation at room temperature for 22 hours. The endpoint titer was determined using the last well to show 50% agglutination.
  • p68 ELISA titers The recombinant p68 antigen was captured on a 96 well microtiter plate coated with a polyclonal antiserum specific to the Bordetella p68 antigen. Serial two fold dilutions of the canine serum were added to the plate and incubated. Positive and negative controls at a 1:1000 dilution were included on each plate. A peroxidase labeled affinity purified goat anti-dog IgG indicator conjugate was used to detect antibodies specific for the rp68 antigen. A chromogenic substrate ABTS was then added and the plate read when the positive control wells had an O.D. of 1.2 ⁇ 0.2. The titer of a given sample was calculated as the reciprocal of the last dilution with an optical density greater than the mean of the negative control serum dilution plus five standard deviations.
  • SAA titers The canine Serum Amyloid A was captured on a 96 well microtiter plate coated with a monoclonal anti-canine SAA antibody. Diluted samples of the canine serum were added to the plate and incubated. A reference standard was added to obtain a standard curve from 0.31 ng/ml to 20 ng/ml. A biotin labeled anti-canine antibody conjugate was added. Following the incubation of the biotin labeled anti-canine antibody, a peroxidase conjugated streptavidin was added. A chromogenic substrate TMB was added and the plate was read after 30 minutes. The concentration of Serum Amyloid A was determined by comparison the sample to the standard curve and multiplication by the appropriate dilution factor.
  • Injection site reactions are summarized in Tables 22-25. Injection site information was not collected for Dog 81595 on Day 21 for Group I due to technical oversight. The protocol was amended so that injection site reaction data was not collected for dogs in Group II on Day 22 therefore, summary of data from Day 22 contains only information from the eight dogs per treatment group in Group I. Injection site reactions were not observed for any dog receiving an IM treatment. Injection site measurements were minimal for both SC vaccinated treatment groups (T03 and T05).
  • Rectal temperature measurements are summarized in Tables 27 and 28. The protocol was amended so that rectal temperature data were not collected for Group II dogs on Day 22, therefore, summary of data from Day 22 contains only information from the dogs per treatment group in Group I.
  • TABLE 27 Least squares mean of rectal temperature (° C.) in dogs following saline or p68 Bordetella vaccination (post first vaccination a ) Rectal Temperature(° C.) b Day of Study Treatment 0 1 2 3 T01 saline SC 38.5 38.4 38.3 38.2 T02 saline IM 38.4 38.2 38.4 38.2 T03 60 ⁇ g SC 38.4 38.5 38.2 38.3 T04 60 ⁇ g IM 38.4 38.5 38.4 38.4 T05 15 ⁇ g SC 38.5 38.5 38.2 38.2 T06 15 ⁇ g IM 38.5 38.4 38.3 38.4
  • Day 79 contains combined data from Day 79 (Group I) and Day 81 (Group II), Day 111 corresponds to Day 110 (Group II) and Day 111 (Group I) and Day 169 corresponds to Day 169 (Group II) and Day 170 (Group I).
  • Day 79 contains combined data from Day 79 (Group I) and Day 81 (Group II)
  • Day 111 corresponds to Day 110 (Group II)
  • Day 111 Group I
  • Day 169 corresponds to Day 169 (Group II) and Day 170 (Group I).
  • data analysis was not performed on p68 ELISA data beyond Day 50.
  • Aerosol challenge for both groups occurred 181 days following the second vaccination.
  • coughing criteria was amended to approximately 45-minute observations, approximately twelve hours apart on the third through eighth day following challenge. Coughing observations are summarized in Tables 32 and 33.
  • SAA values were determined on Days 0, 1, 3, 5, 7 and 9 following challenge. Serum Amyloid A values are presented in Table 35 and represented in FIG. 4. TABLE 35 Geometric mean and standard errors of Serum Amyloid A titers in unvaccinated and p68 Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica Geometric Mean and Standard Errors of Serum Amyloid A a Day of Study 201 202 204 206 208 210 Std. Std. Std. Std. Std.
  • the study was designed to demonstrate the safety and six-month efficacy of a recombinant p68 Bordetella vaccine in dogs. Safety of both the 15 ⁇ g/dose and the 60 ⁇ g/dose vaccine was demonstrated. The efficacy and 6 month duration of immunity of the 15 ⁇ g/dose was well supported in the study.
  • VANGUARD® Plus 5/CV-L is a freeze-dried preparation of attenuated strains of CD virus, CAV-2, CPI virus, CPV, and inactivated whole cultures of L. canicola and L. icterohaemorrhagiae , plus a liquid preparation of inactivated CCV with an adjuvant. All viruses were propagated on established cell lines. The CPV fraction was attenuated by low passage on the canine cell line which gave it the immunogenic properties capable of overriding maternal antibody interference at the levels indicated in Table 38. The liquid component was used to rehydrate the freeze-dried component, which had been packaged with inert gas in place of vacuum.
  • CAV-2 vaccine cross-protects against ICH caused by CAV-1.
  • CAV-2 not only protects against ICH, but against CAV-2 respiratory disease as well.
  • Canine adenovirus type 2 challenge virus was not recovered from CAV-2-vaccinated dogs in tests conducted.
  • VANGUARD® Plus 5/CV-L The CPV fraction in VANGUARD® Plus 5/CV-L was subjected to comprehensive safety and efficacy testing. It was shown to be safe and essentially reaction-free in laboratory tests and in clinical trials under field conditions. Product safety was further demonstrated by a backpassage study which included oral administration of multiple doses of the vaccine strain to susceptible dogs, all of whom remained normal.
  • B. bronchispetica (strain 110H) was harvested from a 48 hour Bordet-Gengou blood agar spread plates by washing the plate surface with 5 to 10 ml heat extraction buffer. Alternatively, cells grown in both culture (Charlotte Parker Defined Medium) were harvested by centrifugation discarding the supernatant fraction. Harvested cells were suspended in 25 mM Tris-HCL, pH 8.8 and incubated at 60° C. for 1 hour. Cell debris was separated from heat extract by centrifugation at 20,000 ⁇ g at 4° C. fro 30 minutes. Sodium azide (0.01%) was added to the heat extracted supernatant fraction which was then further clarified by microporous filtration.
  • Monoclonal antibody affinity resin was prepared by conjugation of monoclonal antibody (designated Bord 2-7) to CNBr-activated Sepharose 4B using standard procedures. Approximately 30.35 mg of monoclonal antibody was conjugated to 1 gram of affinity resin. Clarified heat extracted supernatant fraction (above) and Bord 2-7 affinity resin was combined at an approximate ratio of 1 liter extract to 20 ml resin.
  • Binding of the native p68 to the resin was facilitated by incubating the mixture at ambient temperature, with gentle shaking, overnight, followed by resin settling and aspiration of the supernatant fraction.
  • the resin was then packed into a 2.6 cm diameter column and the column washed sequentially with PBS, pH 7.5 and 10 mM phosphate buffer, pH 8.0 at a flow rate of 5 ml/min.
  • PBS pH 7.5 and 10 mM phosphate buffer, pH 8.0 at a flow rate of 5 ml/min.
  • absorbance at 280 nm reached a baseline level
  • bound material was eluted using 100 mM triethylamine and fractions under the single large peak of 280 nm absorbance were collected and tested for the presence of p68 by ELISA. Fractions containing p68 were pooled and dialyzed against PBS to remove triethylamine.
  • An experimental vaccine serial formulated was formulated to contain approximately 100 micrograms of purified p68 and 1% aluminum hydroxide gel. Formalin (0.01%) was used as a preservative in a final vaccine dose volume of 1 mL.
  • Challenge material was prepared essentially as described in examples above.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Pulmonology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
US10/767,809 2003-01-29 2004-01-29 Canine vaccines against Bordetella bronchiseptica Abandoned US20040185062A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/767,809 US20040185062A1 (en) 2003-01-29 2004-01-29 Canine vaccines against Bordetella bronchiseptica
US10/959,757 US20050089533A1 (en) 2003-01-29 2004-10-06 Canine vaccines against Bordetella bronchiseptica
US11/942,843 US7736658B2 (en) 2003-01-29 2007-11-20 Canine combination vaccines
US11/962,699 US20080175860A1 (en) 2003-01-29 2007-12-21 Canine vaccines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44341803P 2003-01-29 2003-01-29
US10/767,809 US20040185062A1 (en) 2003-01-29 2004-01-29 Canine vaccines against Bordetella bronchiseptica

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/959,757 Continuation-In-Part US20050089533A1 (en) 2003-01-29 2004-10-06 Canine vaccines against Bordetella bronchiseptica
US11/942,843 Division US7736658B2 (en) 2003-01-29 2007-11-20 Canine combination vaccines

Publications (1)

Publication Number Publication Date
US20040185062A1 true US20040185062A1 (en) 2004-09-23

Family

ID=32825327

Family Applications (2)

Application Number Title Priority Date Filing Date
US10/767,809 Abandoned US20040185062A1 (en) 2003-01-29 2004-01-29 Canine vaccines against Bordetella bronchiseptica
US11/942,843 Expired - Lifetime US7736658B2 (en) 2003-01-29 2007-11-20 Canine combination vaccines

Family Applications After (1)

Application Number Title Priority Date Filing Date
US11/942,843 Expired - Lifetime US7736658B2 (en) 2003-01-29 2007-11-20 Canine combination vaccines

Country Status (21)

Country Link
US (2) US20040185062A1 (ja)
EP (1) EP1592444B1 (ja)
JP (3) JP2006515024A (ja)
KR (1) KR20050103215A (ja)
CN (1) CN1835767A (ja)
AR (1) AR042935A1 (ja)
AT (1) ATE395077T1 (ja)
AU (1) AU2004208556B2 (ja)
BR (1) BRPI0407032A (ja)
CA (1) CA2513352C (ja)
DE (1) DE602004013723D1 (ja)
ES (1) ES2303046T3 (ja)
HR (1) HRP20050660A2 (ja)
MX (1) MXPA05008051A (ja)
NO (1) NO20053734L (ja)
PL (1) PL378001A1 (ja)
RU (1) RU2005124130A (ja)
TW (1) TW200505476A (ja)
UY (1) UY28171A1 (ja)
WO (1) WO2004067031A1 (ja)
ZA (1) ZA200506073B (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100028379A1 (en) * 2006-12-27 2010-02-04 Pfizer, Inc Methods of vaccine administration
WO2012104821A1 (en) * 2011-02-04 2012-08-09 Pfizer Inc. Immunogenic bordetella bronchiseptica compositions
WO2012104820A1 (en) * 2011-02-04 2012-08-09 Pfizer Inc. Compositions for canine respiratory disease complex
US9023366B2 (en) 2003-07-01 2015-05-05 The Royal Veterinary College Vaccine composition for vaccinating dogs against canine infectious respiratory disease (CIRD)
RU2569457C2 (ru) * 2006-09-11 2015-11-27 Зоитис Пи ЭлЭлСи Эмульсионная вакцина, полученная из обработанного нагреванием бактерина
IT202100014747A1 (it) * 2021-06-07 2022-12-07 Consiglio Nazionale Ricerche Derivati sterolici come nuovi ligandi del recettore dectina-1 nel trattamento terapeutico di patologie correlate a tale recettore

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050089533A1 (en) * 2003-01-29 2005-04-28 Joseph Frantz Canine vaccines against Bordetella bronchiseptica
KR20070008625A (ko) * 2004-04-05 2007-01-17 화이자 프로덕츠 인코포레이티드 미세유체화된 수중유 유화액 및 백신 조성물
EP1871410A2 (en) * 2005-04-07 2008-01-02 Pharmacia & Upjohn Company LLC Formulations and process for production of bordetella bronchiseptica p68 antigen and vaccines
US7682619B2 (en) 2006-04-06 2010-03-23 Cornell Research Foundation, Inc. Canine influenza virus
BRPI0603490B1 (pt) * 2006-07-21 2018-04-24 Universidade Federal De Minas Gerais Vacina recombinante contra a leishmaniose visceral canina
JP5470052B2 (ja) * 2007-02-27 2014-04-16 ゾエティス・ダブリュー・エルエルシー 動物の感染症を治療または予防するための免疫原組成物
ES2728949T3 (es) * 2008-06-27 2019-10-29 Zoetis Services Llc Composiciones adyuvantes novedosas
CA2777692A1 (en) * 2009-10-14 2011-04-21 Sanjay Kapil Isolation of a virus related to canine parvovirus-2 from a raccoon
CN103153337A (zh) * 2010-09-28 2013-06-12 共立制药股份有限公司 黏膜佐剂组合物
CN103261214B (zh) * 2010-12-22 2016-10-26 英特维特国际股份有限公司 具有活细菌分离物的用于全身施用的疫苗
BR112014029725B1 (pt) 2012-05-31 2023-09-26 Zoetis Services Llc Usos de coronavírus respiratório canino para proteção contra infecções por b. bronchiseptica
CZ307883B6 (cs) * 2013-01-29 2019-07-24 Bioveta, A.S. Multivalentní vakcína k imunoprofylaxi infekčních onemocnění psů
WO2015042369A2 (en) 2013-09-19 2015-03-26 Zoetis Llc Oil-based adjuvants
PL3244920T3 (pl) 2015-01-16 2023-09-25 Zoetis Services Llc Szczepionka przeciw pryszczycy
BR112017018017A2 (pt) * 2015-02-26 2018-04-10 Thevax Genetics Vaccine Co Ltd composição de vacina compreendendo uma proteína imunogênica e adjuvantes de combinação para uso na promoção de respostas de células t específicas de antigenos
CN106399260B (zh) * 2015-07-31 2021-03-02 北京大北农科技集团股份有限公司动物医学研究中心 一种犬瘟热、细小病毒病二联活疫苗及其制备方法
TW202206098A (zh) * 2020-08-11 2022-02-16 美商碩騰服務公司 抗冠狀病毒疫苗

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4567042A (en) * 1983-06-15 1986-01-28 American Home Products Corporation Inactivated canine coronavirus vaccine
US4567043A (en) * 1983-06-15 1986-01-28 American Home Products Corporation (Del.) Canine corona virus vaccine

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3950512A (en) * 1972-07-25 1976-04-13 Pitman-Moore, Inc. Animal vaccines
GB2048669A (en) * 1979-05-18 1980-12-17 Moskov Nii Virusnykh Preparato Canine distemper vaccines
GB8502399D0 (en) * 1985-01-31 1985-03-06 Akzo Nv Canine parvovirus vaccines
NZ230747A (en) * 1988-09-30 1992-05-26 Bror Morein Immunomodulating matrix comprising a complex of at least one lipid and at least one saponin; certain glycosylated triterpenoid saponins derived from quillaja saponaria molina
EP1016416A3 (en) * 1990-07-06 2002-10-23 Wyeth Vaccine against lyme disease and a challenge model for evaluating vaccine efficacy
GB9106568D0 (en) * 1991-03-27 1991-05-15 Wellcome Found Recombinant antigen
ZA927037B (en) * 1991-09-30 1993-03-29 Akzo Nv Respiratory disease vaccine for cats.
JP2521226B2 (ja) * 1993-01-28 1996-08-07 株式会社微生物化学研究所 犬用多種混合ワクチン
UA56132C2 (uk) * 1995-04-25 2003-05-15 Смітклайн Бічем Байолоджікалс С.А. Композиція вакцини (варіанти), спосіб стабілізації qs21 відносно гідролізу (варіанти), спосіб приготування композиції вакцини
AUPO517897A0 (en) * 1997-02-19 1997-04-11 Csl Limited Chelating immunostimulating complexes
GB9718901D0 (en) * 1997-09-05 1997-11-12 Smithkline Beecham Biolog Vaccine
AU769539B2 (en) * 1999-01-29 2004-01-29 Zoetis Services Llc Adjuvants for use in vaccines

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4567042A (en) * 1983-06-15 1986-01-28 American Home Products Corporation Inactivated canine coronavirus vaccine
US4567043A (en) * 1983-06-15 1986-01-28 American Home Products Corporation (Del.) Canine corona virus vaccine
US4824785A (en) * 1983-06-15 1989-04-25 American Home Products Corp. (Del) Canine corona virus vaccine

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023366B2 (en) 2003-07-01 2015-05-05 The Royal Veterinary College Vaccine composition for vaccinating dogs against canine infectious respiratory disease (CIRD)
RU2569457C2 (ru) * 2006-09-11 2015-11-27 Зоитис Пи ЭлЭлСи Эмульсионная вакцина, полученная из обработанного нагреванием бактерина
US11524067B2 (en) 2006-12-27 2022-12-13 Zoetis Services Llc Methods of vaccine administration
KR101099883B1 (ko) * 2006-12-27 2011-12-28 화이자 프로덕츠 인코포레이티드 백신 투여 방법
US11524068B2 (en) * 2006-12-27 2022-12-13 Zoetis Services Llc Methods of vaccine administration
US20100028379A1 (en) * 2006-12-27 2010-02-04 Pfizer, Inc Methods of vaccine administration
AU2007343130B2 (en) * 2006-12-27 2012-12-20 Zoetis Services Llc Methods of vaccine administration
AU2007343130C1 (en) * 2006-12-27 2017-11-09 Zoetis Services Llc Methods of vaccine administration
WO2012104820A1 (en) * 2011-02-04 2012-08-09 Pfizer Inc. Compositions for canine respiratory disease complex
EP3858381A1 (en) * 2011-02-04 2021-08-04 Zoetis Services LLC Immunogenic bordetella bronchiseptica compositions
WO2012104821A1 (en) * 2011-02-04 2012-08-09 Pfizer Inc. Immunogenic bordetella bronchiseptica compositions
IT202100014747A1 (it) * 2021-06-07 2022-12-07 Consiglio Nazionale Ricerche Derivati sterolici come nuovi ligandi del recettore dectina-1 nel trattamento terapeutico di patologie correlate a tale recettore
WO2022259129A1 (en) * 2021-06-07 2022-12-15 Consiglio Nazionale Delle Ricerche Sterol derivatives as new dectin-1 receptor ligands in the therapeutic treatment of diseases related to said receptor

Also Published As

Publication number Publication date
EP1592444A1 (en) 2005-11-09
AU2004208556B2 (en) 2009-06-04
CA2513352C (en) 2010-10-12
EP1592444B1 (en) 2008-05-14
ATE395077T1 (de) 2008-05-15
ES2303046T3 (es) 2008-08-01
CN1835767A (zh) 2006-09-20
JP2011137017A (ja) 2011-07-14
WO2004067031A1 (en) 2004-08-12
RU2005124130A (ru) 2006-01-20
PL378001A1 (pl) 2006-02-20
US20080226670A1 (en) 2008-09-18
ZA200506073B (en) 2006-05-31
JP2006515024A (ja) 2006-05-18
NO20053734D0 (no) 2005-08-03
KR20050103215A (ko) 2005-10-27
NO20053734L (no) 2005-10-26
DE602004013723D1 (de) 2008-06-26
HRP20050660A2 (en) 2006-02-28
JP5319882B2 (ja) 2013-10-16
UY28171A1 (es) 2004-08-31
TW200505476A (en) 2005-02-16
JP2007161718A (ja) 2007-06-28
US7736658B2 (en) 2010-06-15
AR042935A1 (es) 2005-07-06
BRPI0407032A (pt) 2006-01-17
AU2004208556A1 (en) 2004-08-12
CA2513352A1 (en) 2004-08-12
MXPA05008051A (es) 2005-09-21

Similar Documents

Publication Publication Date Title
US7736658B2 (en) Canine combination vaccines
EP1799253B1 (en) Multivalent canine vaccines against leptospira bratislava and other pathogens
US11524067B2 (en) Methods of vaccine administration
US20060251674A1 (en) Formulations and process for production of Bordetella bronchiseptica P68 antigen and vaccines
AU2012244091A1 (en) Multivalent canine vaccines against leptospira bratislava and other pathogens
ARIMITSU et al. Cross antigenicities of Leptospira interrogans serovar copenhageni Shibaura strain for preparing biological products in Japan
JP2008546637A (ja) ルデテラ・ブロンキセプチカｐ６８抗原及びワクチン製造のための製剤及び製法プロセス

Legal Events

Date Code Title Description
AS Assignment

Owner name: PFIZER INC., NEW YORK

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DOMINOWSKI, PAUL J.;FRANTZ, JOSEPH C.;KREBS, RICHARD L.;AND OTHERS;REEL/FRAME:015396/0084;SIGNING DATES FROM 20040505 TO 20040524

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION

AS Assignment

Owner name: PFIZER INC., NEW YORK

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:PFIZER PRODUCTS INC.;REEL/FRAME:029041/0099

Effective date: 20120928

AS Assignment

Owner name: PFIZER INC., NEW YORK

Free format text: CORRECTION BY DECLARATION OF INCORRECT NUMBERS RECORDED AT REEL 029041 FRAME 0099;ASSIGNOR:PFIZER INC.;REEL/FRAME:029926/0644

Effective date: 20130131