TW393446B - Process for deactivating or destroying microorganisms - Google Patents
Process for deactivating or destroying microorganisms Download PDFInfo
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- TW393446B TW393446B TW084114140A TW84114140A TW393446B TW 393446 B TW393446 B TW 393446B TW 084114140 A TW084114140 A TW 084114140A TW 84114140 A TW84114140 A TW 84114140A TW 393446 B TW393446 B TW 393446B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/03—Electric current
- A61L2/035—Electrolysis
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/467—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection; by electrooxydation or by electroreduction
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- C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/46104—Devices therefor; Their operating or servicing
- C02F1/4618—Devices therefor; Their operating or servicing for producing "ionised" acidic or basic water
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
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- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
經濟部中央標準局員工消費合作社印製 A7 ___ B7 一 五、發明説明(丨) 本發明有關謀求殺菌、殺病毒、或消毒或滅菌之微生 物之情化,破壞之方法。 含綠膿菌、大腸菌等細菌及其他真菌之細菌或微小的 病毒等等,將微生物有效破壞進行所有殺菌、消毒、滅菌 處理之方法被提議。但是,此些任一微生物特性均未被充 份討論。例如公知的有電氣化學性的殺菌方法,此法係將 微生物接觸電極表面、誘電體而阻礙其生物化學反應以破 壞微生物爲前提者。通常,細菌之一般表面爲帶負電被陽 極吸引,接觸後引起菌體破壞此乃事實,但,若菌體量多 時,則菌體內部之原形質(蛋白液)會貯留使細菌呈現蛋 白質下之保護狀態,由於此形成某種電性護罩狀態造成殺 菌能力減低,易發生電極保存管理之問題,因此實用上發 生困難。 因此,本發明者重視細菌之破壞狀況,考慮到何種機 制下破壞微生物等微生物之特性,進行各種研究之結果, 確定了細菌在與陽極接觸之前,在噴出細胞質之同時進行 崩潰現象。由此現象之確認,得知此乃微生物電容器之菌 體於電場接受荷電,以致超越靜電容量時,引起細胞膜之 破壞,死滅者,基於此發明而被提案。 有關此發明之惰化,破壞微生物之方法係謀求第1: 於微生物上藉著具電能之液體、氣體或固體付加電能;第 2:藉此使微生物所有之細胞內外之靜電容量界限超過以 提昇其電荷,及/或促進微生物之細胞內外之滲透,及第 3:引發微生物細胞之不可逆變化及/或使微生物細胞之 本紙張尺度適用中國國家標準(CNS ) A4規格(210X 297公釐) ' -4 - I---:--,---- I —------ΐτ------Λ (請先閱讀背面之注意事項寫本頁) . - A7 ______B7___ 五、發明説明(2 ) 境界膜之爆裂性破壞。 具電能之液體以採用電荷水爲佳,於此電荷水中對微 生物付加電能。此電荷水可爲荷電水、蓄電水、性能水、 電分解處理水、強氧化電位水、強酸性電解生成水溶液、 電離水、非電離水或帶電水均可。又,具電能之氣體係具 電荷之氣體,具電能之固體係蓄電體者。 再者爲可使用電極,此時情化並破壞微生物之方法可 以第1於含微生物之液體透過電極通電後,對於電極呈非 接觸狀態之微生物付加電能者,第2 :使微生物所有之細 胞內外之靜電容量超過而提昇其電荷,及/或促進微生物 細胞內外之滲透,及第3引發微生物細胞之不可逆變化及 /或使微生物細胞之境界膜爆裂性破壞。另外,藉由電極 之通電,可以電媒性通電或非電媒性通電。 經濟部中央標準局員工消費合作社印裝 由以上及以下所說明,所謂「微生物」係含真菌之細 菌或細小之病毒等之總稱。又,所謂「境界膜」係細胞原 形質經外界遮蓋之境界膜,外膜、界面膜、原形質膜、細 胞壁等之總稱,在廣範概念下所使用者。另外,所謂「爆 裂性破壞」係指細胞膜彈性強時,引起強烈膜收縮而內容 物(原形質)之噴出造成多面放射狀散亂狀態、細胞膜之 彈性弱且細胞內壓過高時,局部破壞並行噴出周邊散亂爲 少之狀態,或直接表示同樣細胞膜彈性弱且相對的外壓低 時,產生並行噴射與周邊散亂狀態等,但並非僅限定於此 ,亦含間接性膨壓破壞,而廣泛總稱爲包含菌體破壞之一 般現象之溶解、脫水、凝固、融解、穿孔等破壞狀態。 本紙張尺度適财關家標準(€叫八4規格(21()'/297公董) ' -5 - A7 ___B7 五、發明説明(3 ) 更者所謂「電荷水」係指荷電水、蓄電水、性能水、 電分解處理水、強氧化電位水、強酸性電解生成水溶液、 電離水、非電離水或帶電水等之總稱。又「通電」係指不 僅限於加入導電質(N a C β其他)之溶液的電媒性通電 、亦包含純水、精製水等一般無導電性之溶液的非電媒性 通電亦包含之。另外,本發明人確定在後者時,於純水或 精製水使生菌漂浮而通電時與電媒性通電相較後尙可取得 微小電流》此乃菌體間一種所謂跳躍傳導作用(非電媒性 通電之一種),而明瞭菌體往陽極方向形成一定流。 又,「滲透」(Osmosis )係指在爲水滲透膜之溶 質不滲透性膜所分隔區分下使溶液中之水往濃度高位側方 向移動之現象,若滲透壓髙則與境界膜之破壞相關。再者 ,「不可逆變化」係指造成阻礙境界膜物質的交換之狀態 ,境界膜自身的變性、變質或原形質之變性、變質之狀態 之變化的總稱。 發明之第1實施形態; 經濟部中央標準局員工消費合作社印製 ' 選擇微生物中黃色葡萄球菌、大腸菌、綠膿菌、念珠 菌及流行感冒菌,於複數之標本(preparation )上預 先供給0. 0 1 mj?之微量精製水或自來水中使其各自浮 遊,再以結合菌噴射器將強氧化電位水一滴滴滴入,其結 果,以倒立型系統顯微鏡、IX— 70 ( Olympas公司 製)附設微分差干涉裝置,更與1 4吋監視器(附C CD )組合使最終倍率4 5 0 0倍之裝置,檢測立體層狀之惰 本紙張尺度適用中國國家標準(CNS ) A4規格(210X297公釐) -6 ~ A7 393446 ______ B7_ 五、發明説明(4 ) 化並破壞微生物之狀態。 強氧化竜位水之一滴量爲〇· 0 1mJ2,一滴滴滴入 分5階段觀察之,確定了上述微生物不管任何狀況下,在 1滴70%、2滴80%、3滴99%之微生物細胞破裂 後成爲碎片。再滴下4滴時碎片增加,最後5滴則微生物 細胞呈接近1 0 0%破滅狀態。 綠腆菌與其他微生物相較下爲較強細菌,但,由上述 與其他菌種同樣狀況下其微生物細胞之破滅則值得重視。 但,念珠菌原本即不動之菌種,因而境界破壞之目視確認 有困難,由於幾乎所有之菌體搖動性均停止,因此亦可確 定念珠菌之惰化或被破壞。又,3滴滴下時,因菌種不同 ,而有未破裂者或不動狀態之菌者,又有僅極少量之漂浮 性菌體。 第2實施形態: 經濟部中央標準局貝工消费合作社印袈 於複數之標本上準備供給0. Olmj?之微量精製水 與供給0. Olmj?微量之自來水,選擇微生物之黃色葡 萄球菌、大腸菌、綠膿菌、念珠菌及流感菌使各自漂浮之 。於精製水中以0· 2mA — 0. 3mA :12V之電能 ,自來水中則以0 . 5 m A : 1 2 V之電能,藉各白金電 極付加後,確定9 9 %之菌體細胞膜破裂》 第3實施形態; 做爲具有電能之氣體,則使用以等離子體放電產生之 本纸浪尺度速用中國國家梯準(CNS ) A4規格(2丨0X297公釐) ~ 7 -
Claims (1)
- 經濟部智慧財產局員工消费合作社印製 1 . 一種惰化並破壞微生物之方法,其特徵爲對於微 生物藉由可介著具電能之液體、氣體、固體付與2 0 0 mV- 1800mV的帶電電荷度(ORP)(電能), 提昇該電荷使其超越微生物所具有之細胞內外靜電容置的 界限、且促進微生物細胞內外之滲透,使微生物細胞引發 不可逆變化及/或爆裂性地破壞微生物細胞之境界膜。 2. 如申請專利範圍第1項之惰化並破壤微生物之方 法,其中該具電能之液體爲電荷水,於此電荷水水中放入 微生物付予電能。 3. 如申請專利範困第2項之惰化並破壞微生物之方 法,其中該電荷水爲荷電水、蓄電水、性能水、電分解處 理水、強氧化電位水、強酸性電解生成水溶液、電離水、 非電離水或帶電水的任一。 4. 如申請專利範圍第1項之惰化並破壞微生物之方 法,其中該具電能之氣體爲具電荷之氣體。 5. 如申請專利範圏第1項之惰化並破壞微生物之方 法,其中該具電能之固體爲蓄電髖。 6. —種惰化並破壞微生物之方法,其特徴爲介由電 極通電於含微生物之液體後,對於非接觸於電極之狀態的 微生物付予200mV_l 800mV的帶電電荷度( 本紙張尺度適用中國國家標準<CNS>A4規格(210 X 297公釐) t —ί h--I ^--------β--I I-----線 I ^/1. (請先M讀背面之注意事項再填寫本頁)經濟部智慧財產局員工消费合作社印製 1 . 一種惰化並破壞微生物之方法,其特徵爲對於微 生物藉由可介著具電能之液體、氣體、固體付與2 0 0 mV- 1800mV的帶電電荷度(ORP)(電能), 提昇該電荷使其超越微生物所具有之細胞內外靜電容置的 界限、且促進微生物細胞內外之滲透,使微生物細胞引發 不可逆變化及/或爆裂性地破壞微生物細胞之境界膜。 2. 如申請專利範圍第1項之惰化並破壤微生物之方 法,其中該具電能之液體爲電荷水,於此電荷水水中放入 微生物付予電能。 3. 如申請專利範困第2項之惰化並破壞微生物之方 法,其中該電荷水爲荷電水、蓄電水、性能水、電分解處 理水、強氧化電位水、強酸性電解生成水溶液、電離水、 非電離水或帶電水的任一。 4. 如申請專利範圍第1項之惰化並破壞微生物之方 法,其中該具電能之氣體爲具電荷之氣體。 5. 如申請專利範圏第1項之惰化並破壞微生物之方 法,其中該具電能之固體爲蓄電髖。 6. —種惰化並破壞微生物之方法,其特徴爲介由電 極通電於含微生物之液體後,對於非接觸於電極之狀態的 微生物付予200mV_l 800mV的帶電電荷度( 本紙張尺度適用中國國家標準<CNS>A4規格(210 X 297公釐) t —ί h--I ^--------β--I I-----線 I ^/1. (請先M讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消费合作社印製 993446 § __D8 六、申請專利範圍 ORP)(電能)而提昇該電荷使其超越微生物所具有之 細胞內外靜電容量的界限,使微生物引發不可逆變化、及 /或爆裂性地破壞微生物細胞之境界膜。 7 _ —種惰化並破壤微生物之方法,其特徵爲介由電 極通電於含微生物之液體後•對於非接觸於電極之狀態的 微生物付予2 0 OmV— 1 8 0 OmV的帶電電荷度( 〇 R P )(電能),促進微生物細胞內外之滲透,使微生 物引發不可逆變化,及/或爆裂性地破壤微生物細胞之境 界膜。 8. —種惰化並破壊微生物之方法,其特徵爲介由電 極通電於含微生物之液體,對於非接觸於電極之狀態的微 生物付予2 0 OmV— 1 8 0 OmV的帶電電荷度( ORP)(電能),提昇該電荷使其超越微生物所具有之 細胞內外靜電容量的界限,並且促進微生物細胞內外之滲 透,使微生物引發不可逆變化,及/或爆裂性地破壊微生 物細胞之境界膜。 9. 如申請專利範圍第6〜8項中任一項之惰化並破 壞微生物之方法,其中該通電爲電媒性通電或非電媒性通 電· 1 0 .—種惰化並破壤微生物之方法,其特徴爲對於 微生物藉由可介著具電能之液體、氣體、固體付與0.1 m A - 1 . 2mA :8V — 16V下瞬間的電能,提昇該 電荷使其超越微生物所具有之細胞內外靜電容量的界限、 且促進微生物細胞內外之滲透’使微生物細胞引發不可逆 本紙張尺度適用中國國家櫟準(CNS>A4規格(210 297公釐) (請先閲讀背面之注意事項再填寫本頁) ^!!訂_! •線 A8 … B8 393446_g _ 六、申請專利範圍 變化及/或爆裂性地破壞微生物細胞之境界膜。 1 1 . 一種惰化並破壤微生物之方法,其特徵爲介由 電極通電於含微生物之液體後,對於非接觸於電極之狀態 的微生物付予0.1mA-1. 2mA:8V—16V下 瞬間的電能而提昇該電荷使其超越微生物所具有之細胞內 外靜電容量的界限,使微生物引發不可逆變化、及/或爆 裂性地破壊微生物細胞之境界膜。 1 2 .—種惰化並破壞微生物之方法,其特徵爲介由 電極通電於含微生物之液體後,對於非接觸於電極之狀態 的微生物付予0.1mA—1. 2mA:8V—16V下 瞬間的電能,促進微生物細胞內外之滲透,使微生物引發 不可逆變化,及/或爆裂性地破壤微生物細胞之境界膜。 經濟部智慧財產局wr工消费合作杜印契 (請先閱讀背面之注意事項再填寫本頁) 1 3 . —種惰化並破壞微生物之方法,其特徵爲介由 電極通電於含微生物之液體,對於非接觸於電極之狀態的 微生物付予0.1mA-1. 2mA:8V—16V下瞬 間的電能,提昇該電荷使其超越微生物所具有之細胞內外 靜電容量的界限,並且促進微生物細胞內外之滲透,使微 生物引發不可逆變化,及/或爆裂性地破壞微生物細胞之 境界膜。 本紙張尺度適用中國國家標準(CNS)A4規格<210 X 297公釐>
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CN110491678A (zh) * | 2018-05-14 | 2019-11-22 | 深圳市中科摩方科技有限公司 | 一种表面超电容修饰的材料及其制备方法和应用 |
CN109908035A (zh) * | 2019-04-25 | 2019-06-21 | 山西纳安生物科技有限公司 | 一种基于牡丹籽油的祛斑面膜 |
CN112556078A (zh) * | 2020-11-11 | 2021-03-26 | 山东青洁能环保有限公司 | 感应正静电超低容量雾化电位水消毒除臭方法及设备 |
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US5308507A (en) * | 1992-08-04 | 1994-05-03 | Los Alamos Technical Associates, Inc. | Method and apparatus for removing organic contaminants |
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US5635059A (en) * | 1994-10-20 | 1997-06-03 | Aqua-Ion Systems, Inc. | Method and apparatus for water treatment and purification using gas ion plasma source and disinfectant metal ion complexes |
US5695650A (en) * | 1995-11-02 | 1997-12-09 | Held; Jeffery S. | Method for dewatering previously-dewatered municipal waste-water sludges using high electrical voltage |
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1995
- 1995-09-28 JP JP7251623A patent/JPH0994288A/ja active Pending
- 1995-12-28 CN CN95119096A patent/CN1146489A/zh active Pending
- 1995-12-29 EP EP95309545A patent/EP0765843A1/en not_active Withdrawn
- 1995-12-29 KR KR1019950066743A patent/KR970015473A/ko not_active Application Discontinuation
- 1995-12-29 TW TW084114140A patent/TW393446B/zh active
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1996
- 1996-01-04 AU AU40830/96A patent/AU694292B2/en not_active Ceased
- 1996-01-04 NZ NZ280785A patent/NZ280785A/en unknown
- 1996-01-09 CA CA002166855A patent/CA2166855C/en not_active Expired - Fee Related
- 1996-01-15 FI FI960193A patent/FI960193A/fi not_active Application Discontinuation
- 1996-01-18 IL IL11681296A patent/IL116812A0/xx unknown
- 1996-01-18 ZA ZA96407A patent/ZA96407B/xx unknown
- 1996-01-19 SG SG1996000303A patent/SG48713A1/en unknown
- 1996-01-19 NO NO960242A patent/NO960242L/no unknown
- 1996-01-23 PE PE1996000053A patent/PE26297A1/es not_active Application Discontinuation
- 1996-01-25 AR ARP960101153A patent/AR000805A1/es unknown
- 1996-01-31 MX MX9600439A patent/MX9600439A/es unknown
- 1996-01-31 BR BR9600285A patent/BR9600285A/pt not_active Application Discontinuation
- 1996-01-31 MA MA24145A patent/MA23790A1/fr unknown
- 1996-02-02 CZ CZ96309A patent/CZ30996A3/cs unknown
- 1996-02-14 RU RU96102855A patent/RU2108113C1/ru active
- 1996-04-19 PL PL96313858A patent/PL313858A1/xx unknown
- 1996-09-27 HU HU9602683A patent/HUP9602683A2/hu unknown
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1997
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Also Published As
Publication number | Publication date |
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KR970015473A (ko) | 1997-04-28 |
CA2166855A1 (en) | 1997-03-29 |
SG48713A1 (en) | 1998-05-18 |
RU2108113C1 (ru) | 1998-04-10 |
CA2166855C (en) | 1999-01-19 |
ZA96407B (en) | 1997-01-20 |
MX9600439A (es) | 1997-03-29 |
JPH0994288A (ja) | 1997-04-08 |
AR000805A1 (es) | 1997-08-06 |
FI960193A (fi) | 1997-03-29 |
CZ30996A3 (cs) | 1998-03-18 |
NO960242D0 (no) | 1996-01-19 |
IL116812A0 (en) | 1996-05-14 |
EP0765843A1 (en) | 1997-04-02 |
AU694292B2 (en) | 1998-07-16 |
MA23790A1 (fr) | 1996-10-01 |
NZ280785A (en) | 1997-11-24 |
PE26297A1 (es) | 1997-08-14 |
US5922209A (en) | 1999-07-13 |
NO960242L (no) | 1997-04-01 |
CN1146489A (zh) | 1997-04-02 |
BR9600285A (pt) | 1997-12-23 |
AU4083096A (en) | 1997-04-10 |
FI960193A0 (fi) | 1996-01-15 |
HU9602683D0 (en) | 1996-11-28 |
HUP9602683A2 (en) | 1997-06-30 |
PL313858A1 (en) | 1997-04-01 |
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