200804588 九、發明說明： 【發明所屬之技術領域】 本發明，係有關細胞培養容器及利用 方法。 【先前技術】 細胞死可大別爲凋亡（apoptosis)與壞： 凋亡，係意味著構成多細胞生物體之細 了保持個體良好之態樣所主動引起之、 細胞自殺。在生體內癌化細胞（及其他 細胞）的大部分，幾乎都藉由凋亡持續 大部分之腫瘤的成長都可防患於未然。 過程，會在預先決定的時期、決定的場 計劃的細胞死），此爲生物之形態變化等 由凋亡的結構而發生。例如，由蝌蚪變 消失是因爲凋亡所引起。而蛔蟲則在發 均會被記錄。又如在免疫系統亦有因對 生之細胞除去等，扮演著重要的角色。 反之’由於血行不良、外傷等藉由細 所引起的細胞死，稱爲壞死（n e c r 〇 s i s)， 亡的語源係來自希臘語的「apo -（離開 降）」，具有「（枯葉等從樹上）掉下」之 壞死，係指生物組織的一部分死去。 僅構成身體之一部分的細胞死亡。其原 破壤、化學的損傷、血流的減少等。由 者特別稱爲梗塞。同樣係細胞死，但像 該容器的細胞培養 死（n e c r 〇 s i s)。所謂 胞死法的一種，爲 受到管理、調節的 在內部發生異常的 除去，據此，習知 又，在生物的發生 所發生細胞死（被 的原動力，亦係藉 態爲青蛙時尾巴的 生時所引起的凋亡 自己抗原反應所產 胞內外環境的惡化 與此有所區別。凋 )」與「 ptosis (下 .意義。 與通常的死不同， 因係感染、物理的 於血流減少而引起 血球、皮膚、消化 200804588 管的粘膜上皮正常的細胞、組織陸續地補充而不 能的障礙、組織學的異常者不稱爲壞死。壞死之 由生體的免疫系統最後將其除去，缺損的部分由 織再生或線維化來彌補。 爲了檢查、試驗如此的細胞，通常有必要從組 單獨分離而培養。從組織將細胞單獨分離而用於 驗的方法，在生物技術相關領域成爲不可或缺的 泛地被使用於疾病、病態的診所、新藥的探索及 斷、或動物檢查、植物檢查、環境污染物質的試 獨分離的細胞雖有直接用於試驗的情況，但多數 培養的方法在培養盤或試管中進行培養。在此培 ί了各種檢查。 該等分析通常設定均勻的培養系，改變欲評估 的量，濃度等而觀其效果。因此用於培養之培養 用形成一定且均勻者。此培養容器，一般採用稱 者（細胞培養盤）。作爲培養盤一般使用者】 (schale)、或 6 孔盤（6well plate)、12 孔盤、48 孔 盤等各種板（專利文獻1 )。從最近微量化的流行 始使用由小口徑且多數之培養盤而成的3 8 4孔盤 養盤之底部爲平坦的平板狀。 如此，現在在醫學、生物技術領域等，以培養 織培養爲目的而使用之細胞培養盤（schale)、孔 等，其底面的平板部分作爲培養面而使用。 然而，組織細胞的培養在底部爲平板狀的培養 時，細胞薄薄伸展成爲無方向性的形狀，而具有 會造成機 組織，藉 原來的組 織將細胞 檢查、試 方法。廣 藥效的判 驗等。單 藉由細胞 養系中進 之藥物等 容器也使 爲培養盤 爵培養盤 盤、96孔 ，更有開 。該等培 試驗、組 盤（plate) 盤上進行 無法顯不 200804588 原來在生體內可看到之形狀或機能的問題。例如，在平板 狀的培養盤或培養容器進行脂肪細胞前驅細胞之培養，或 分化誘導時會有無法分化成目的的脂肪細胞之形狀的問 題。又，在肝細胞則又有無法形成球狀聚塊狀之細胞塊的 問題。 從而在平板狀，因爲成爲目的之效率好的細胞繁殖或細 胞分化有困雖，所以有被嘗試在平板狀表面將細胞激素 (c y t 〇 k i n e)或把細胞外矩陣固定化之方法或使用特殊裝置 等方法（專利文獻2、專利文獻3 )。 然而，在平板狀固定細胞繁殖因子或細胞外矩陣之方 法，僅初期可提高效率，而無法持續維持或因固定化方法 煩雜而無法穩定的製造，更有花費成本的問題。又，使用 特殊裝置的方法依據細胞種結果也相異，無法估計穩定的 效率化，或因爲使用大架設的裝置以致操作性不良，更有 花費成本的問題。 又，在使用細胞培養盤（s c h a 1 e)、孔盤（p 1 a t e)之培養試驗 或組織培養，由於從培養細胞所排出之二氧化碳等之PH的 變化，已知對於培養細胞的活性會有很大影響。因此在長 期間培養，由於硏究者定期的（例如每2日1次）更換培 養液，以保持培養細胞的活性。更在最新的硏究例有提案 製作，經常可讓新鮮之培養液循環的生物反應器之新的人 造內臟器官替代模型。 然而，爲了抑制像神經細胞或心肌、骨格肌等不會再生 之組織的壞死（necrosis) ’或皮膚、消化管之粘膜上皮的可 再生組織之細胞死的因子尙不明確’可是現況僅更換新鮮 200804588 的培養液仍無法實現抑制細胞死。 特別是難於培養之培養種的老鼠心肌細胞、神經細胞、 角膜細胞等，現況即使更換新鮮的培養液，對於最初分散 (配置）之細胞數的生存率僅20〜40% 。 近年來，藉由使用矽酮作爲材料之半導體加工技術，或 依據光罩餘刻法製造鎳製蓋印機（s t a m p e r)(原盤）之成形技 術的發達，嘗試從公厘、微米、更至奈米水準的微細化。 然而，藉由該等微細加工技術製造之細胞培養多孔盤等， 雖可樣本的微量化、積體化，但並無提案對於培養困難的 細胞種。 專利文獻1 :日本專利特開平8 — 3225 93號公報 專利文獻2 :日本專利特開平2003 — 1 89843號公報 專利文獻3 :日本專利特開平2005 — 1 43 343號公報 【發明內容】 【發明所欲解決之課題】 如此’在以前之細胞培養容器，細胞採取薄薄伸展的形 狀， 無法顯示在生體內原來發現之形狀或機能，要有效率的 將在生體內原來發現之形狀或機能的細胞有效率之繁殖或 /及分化有困難的問題。 本發明所欲解決之課題，係提供一種據此可效率好，且 在生體內顯示原來發現之形狀或機能可更有效率的繁殖或 /及分化之細胞培養容器及使用該容器的細胞培養方法。 【用以解決課題之手段】 有關本發明之第1態樣的細胞培養容器，係具有凹凸樣 200804588 式的細胞培養容器，其係基於該凹凸樣式之凹凸所形成之 凹部的大小爲培養細胞之相當直徑的1 . 〇倍至4 0倍者。據 此可效率好，且在生體內顯示原來發現之形狀或機能可更 有效率的繁殖或/及分化。 有關本發明之第2態樣的細胞培養容器，係在該細胞培 養容器中，該凹部係基於2階以上之凹凸所形成爲其特徵。 據此可效率好，且在生體內顯示原來發現之形狀或機能可 更有效率的繁殖或/及分化。 有關本發明之第3態樣的細胞培養容器，係在該細胞培 養容器中基於該2階以上之凹凸所形成之凹部的底部之大 小爲培養細胞之相當直徑的1 . 5倍至1 0倍。據此可效率 好，且在生體內顯示原來發現之形狀或機能可更有效率的 繁殖或/及分化。 有關本發明之第4態樣的細胞培養容器，係在該細胞培 養容器中，該2階以上之凹凸之中至少以1階以上所形成 的凹部，與鄰接的其他凹部至少連通1個以上爲其特徵。 據此可效率好，且在生體內顯示原來發現之形狀或機能可 更有效率的繁殖或/及分化。 有關本發明之第5態樣的細胞培養容器，係在該細胞培 養容器中，基於該2階以上之凹凸所形成之凹部的最下階 的高度爲1 # m至1 0 0 // m爲其特徵。據此可效率好，且在 生體內顯示原來發現之形狀或機能可更有效率的繁殖或/ 及分化。 有關本發_之第6態樣的細胞培養容器，係在該細胞培 養容器中，基於該凹凸所形成之凹部的底部具有複數個凹 200804588 槽其大小爲培養細胞之相當直徑的0 · 〇 〇 1至〇 . 9倍 徵。據此可效率好，且在生體內顯示原來發現之形 能可更有效率的繁殖或/及分化。 有關本發明之第7態樣的細胞培養容器，係在該 養容器中，該凹槽的高度爲至50//m爲其特 此可效率好，且在生體內顯示原來發現之形狀或機 有效率的繁殖或/及分化。 有關本發明之第8態樣的細胞培養容器，係在該 養容器中，基於凹凸所形成之凹部的高度爲1//m3 m爲其特徵。據此可效率好，且在生體內顯示原來 形狀或機能可更有效率的繁殖或/及分化。 有關本發明之第9態樣的細胞培養容器，係在該 養容器中，在該凹凸樣式所設置之區域進行表面處 特徵。據此可效率好，且在生體內顯示原來發現之 機能可更有效率的繁殖或/及分化。 有關本發明之第1 0態樣的細胞培養容器，係在該 養容器中，容器原料爲透明材料爲其特徵。據此可 行觀察。 有關本發明之第1 1態樣的細胞培養方法，係於設 細胞培養容器之該凹部注入細胞，而培養該細胞。 效率好，且在生體內顯示原來發現之形狀或機能可 率的繁殖或/及分化。 【發明之效果】 根據本發明，係提供一種據此可效率好，且在生 示原來發現之形狀或機能可更有效率的繁殖或/及 爲其特 狀或機 細胞培 徵。據 能可更 細胞培 :200 // 發現之 細胞培 理爲其 形狀或 細胞培 容易進 置在該 據此可 更有效 體內顯 分化之 -10- 200804588 細胞培養容器及使用該容器的細胞培養方法。 【實施方式】 【發明之最佳實施形態】 組織細胞之培養在現在市場上銷售之細胞培養盤 (schale、或well plate)上進行時，培養細胞會成爲薄薄伸展 之無方向的形狀。硏究者作爲確認細胞活性之方法，雖藉 由老廢物排出之PH的變化，或將排出之二氧化碳介由電化 學感測器，嘗試比較生體組織的測定資料，及在培養盤培 養之細胞測定資料，但現況在生體組織之資料的顯示値， 無法在培養盤再顯現。因此，在市場上銷售之細胞培養盤 上的培養，被判斷培養細胞並未顯示在生體內持有之機能。 因此，在培養盤上形成適合繁殖組織細胞之微細容器樣 式，並在其微細容器樣式內培養細胞，使立體的繁殖細胞， 而能順利發現在生體內所持有之機能的硏究開始進行著。 課題更不待言，係使其細胞有效率的繁殖或/及分化。 依據使用有關本發明之細胞培養容器的培養方法，藉由 設置微細凹凸樣式，可能與生體內同樣立體的生育之外， 由於依據微細凹凸所形成之立體的區劃細胞本身產生的細 胞繁殖因子及/或分化誘導因子等的生理活性化物質可與 生體內類似的濃度培養，所以可能模仿生體內細胞周圍的 環境。微細凹凸樣式，例如，具有複數個側壁，依據該等 側壁所形成之爲了配置培養細胞的複數空間，更，藉由在 側壁設置開口部，而可實現形成複數之空間連通的連結構 造。由於具有複數個側壁，製作複數個空間，而可配合要 求的用途設定空間的大小。 -11- 200804588 依據配合培養的細胞種，設定側壁、空間、開口部的尺 寸，推測可能適用多種多樣的培養系。所謂開口部，係藉 由側壁所形成的空間（凹部）爲了彼此能連通的構造作爲 開口部。 藉由側壁所形成之空間的尺寸，在培養細胞的目的上有 必要最適當的範圍。依據側壁形成的空間若過大，細胞與 在平板上培養同樣，薄薄伸展而無法顯示立體的構造，但 空間若過小，則細胞無法進入其空間。所以空間的尺寸， 因應所培養的細胞種，可收納單一或複數個的範圍較符合 心願。 本發明者等專心硏究的結果，在特定微細樣式的形狀， 藉由防止細胞產生物質的擴散，發現可飛躍的提高培養細 胞的生存率，而逹到本發明。 細胞在其培養過程，會產生纖維結合蛋白（fibronectin)、 層黏蛋白（lanunin)、膠原蛋白、彈性纖維（elastin)等物質。 雖然隨著二氧化碳排出所產生之PH變化，是在培養細胞時 其活性降低的主因，但反之該等產生物質可使用於基質材 料等，也是細胞基質表面的接著、分化/繁殖所需不可缺的 材料。 本發明者等，在細胞培養容器形成複數的微細樣式，藉 由在其樣式的側壁下部培養細胞，成功地可防止細胞產生 物質的擴散，及提高細胞的生存率。 防止細胞產生物質的擴散，及提高細胞的生存率爲目 的，將來更期待提高細胞培養株的生產力。從而，在本發 明之微細樣式，在培養面積內如何確保側壁下部的長度成 -12- 200804588 爲問題。 側壁間的下部寬度，單一細胞的最小尺寸爲4〜5 // m，所 以期望例如 2 // m以上。 側壁的寬度，爲了確保在培養面積內側壁下部的長度， 雖盡量期望狹窄，但從工業技術上可重現的觀點，例如期 望 1 μ m以上。 側壁的高度，從抑制產生物質之擴散的觀點，例如期望 1 // m以上。 各級凸部的寬高比，依防止產生物質的擴散爲目的，雖 盡量期望高，但從工業技術上可重現的觀點，高/寬比期 望從1/ 1〜20/ 1之中選擇。 更，有關本實施例之細胞培養容器，對於成爲替代人造 內臟器官之生物反應器等亦有效。由於將基板重疊，使培 養液形成環流之空間時，爲了防止培養細胞產生物質的擴 散與側壁直行之方式設置比側壁高的橋樑，使培養液朝與 側壁直行的方向流動，可防止從培養細胞放出二氧化碳而 引起之PH的變化，及培養細胞產生物質的擴散。 以下，根據圖式詳細說明本發明之形態的細胞培養容器。 發明之實施形態1 以下，有關本發明之實施形態1的細胞培養容器的具體 形狀用第1圖及第2圖加以說明。第1圖係顯示有關本實 施形態之細胞培養容器構成的俯視圖，第2圖係第1圖之A 一 A ’ 的剖視圖。當然第1圖及第2圖顯示之構成，係有 關本發明之細胞培養容器的一例，並不限定圖示之構成。 細胞培養容器，係形成凹凸樣式。而凹凸樣式係形成2 •13- 200804588 階之階級。因此，在細胞培養容器的培養面，形成2階之 凸部1 3。即，形成格子狀之第1凸部1 1之上面長方體狀的 第2凸部1 2形成矩陣狀。藉由該第1凸部1 1及第2凸部 1 2形成之空間爲凹部14。即，相鄰接之第1凸部n之間 的空間及第2凸部1 2之間的空間成爲凹部1 4。藉由第1 凸部1 1的側壁1 5與鄰接之第1凸部1 1的側壁之間及第2 凸部1 2的側壁1 6與鄰接之第2凸部1 2的側壁1 6之間的 空間，形成爲了培養細胞的凹部14。第1凸部1 1的側壁 1 5及第2凸部1 2的側壁1 6對於底面大致形成垂直。因此 凹部1 4具有2階凹凸的階級狀。 第1凸部1 1，如第1圖所示配置成像包圍矩形狀之凹部 14的四邊之格子狀。第2凸部1 2，係在鄰接之凹部14之 間的第1凸部1 1上面配置成島狀。第2凸部1 2，係對矩形 狀之凹部1 4的四邊分別設置。此外，凹部1 4的形狀不限 於矩形狀，亦可爲多角形狀、圓形狀、隋圓形狀或該等複 合形狀等。 此外，理想係凹凸樣式爲2階以上，凹部14由2階以上 凹凸所形成。即，凹部1 4亦可爲2階以上的階級狀。當然， 凹部14亦可爲1階，3階以上也行。據此，可成爲適合培 養的形狀。 依據凹凸樣式所形成之凹部14的大小（寬、深度）爲培 養細胞之相當直徑的1 .〇倍至40倍較理想。即’鄰接之凸 部1 3之間的間隔爲培養細胞之相當直徑的1 ·〇倍至40倍較 理想。換言之，凹部1 4的寬爲培養細胞之相當直徑的1.0 倍至4 0倍較理想。又，由於單一細胞之最小尺寸爲4〜5 // -14- 200804588 m，所以鄰接之第1凸部1 1的側壁1 5之間的寬度，例如2 // m以上較理想。更，凹部14底部的大小（寬、深度）爲 培養細胞之相當直徑的1 .5倍至1 0倍較理想。即，在第1 凸部1 1所形成之空間的寬度爲培養細胞之相當直徑的1 .5 倍至1 0倍較理想。換言之，在鄰接之第1凸部1 1所形成 之間的間隔爲培養細胞之相當直徑的1 · 5倍至1 〇倍較理 想。據此，可成爲適合培養的大小。 又，2階的凹部1 4，理想係與鄰接的其他2階的凹部14 互相連通至少1個以上。在此，第1凸部1 1的上側係與鄰 接之2階的凹部14連通。即，使在鄰接之第2凸部12之 側壁1 6間的空間鄰接之2階的凹部14連通。具體上，係 弟1凸部1 1 §受置成格子狀’在其上面散布島狀的第2凸部 1 2。據此，藉由第2凸部1 2空間不會完全被隔開，所以藉 由第1凸部1 1上的空間鄰接之2階的凹部1 4將連通。又， 凹部1 4爲2階以上時，在1階以上形成的空間，至少與鄰 接的凹部1 4相連通較理想。即，2階以上的凹凸之中至少 形成1階以上的凹部與鄰接的其他凹部1 4互相連通較理 想。此時，藉由最上側的凸部成爲島狀形成在下側的凸部 之上，可使凹部14互相連通。形成凹部14之至少1階的 高度與鄰接的凹部1 4連通。如此，在凹凸樣式設置開口部， 使凹部互相連通較理想。當然，凹部與1個以上之其他凹 部連通即可，據此，可成爲適合培養的形狀。 又，凹部的高度爲1〜200 // m較理想。即第1凸部1 1的 高度與第2凸部1 2的高度之和爲1〜200 μ m較理想。據此， 可成爲適合培養的大小。 •15- 200804588 又，第1凸部1 1的高度爲1〜100 μ m較理想。即，階級 狀之凹部1 4最下階的高度爲1〜1 00 // m較理想。據此’凹 部14之底部的高度成爲1〜100//m，可成爲適合培養的大 小。 凸部1 3的寬度，爲了確保培養面積內之側壁的長度’雖 然希望盡量狹窄，但從工業技術上可能再現的觀點，例如’ 希望爲l//m以上。具體上，第2凸部12的寬度設爲l//m 以上。換言之，構成第2凸部12的2個側壁16間的間隔 爲l//m以上。又，第1凸部11的寬度，大於第2凸部12 即可。即，第2凸部12在第1凸部1 1的上面，所以只要 不比第1凸部1 1露出即可。 凹部1 4的高度，從抑制產生物質擴散的觀點，例如，期 望在l//m以上。即，第1凸部11與第2凸部12之高度的 和在1 // m以上較理想。更佳係使凹部1 4的高度在200 /z m 以下。 第2凸部1 2之寬高比，依防止產生物質擴散的目的，雖 期望盡量高，但從工業技術上可能再現的觀點，高/寬比 期望從1/1〜20/1之中選擇。即，期望第2凸部12之高 度爲第2凸部12之寬度的1〜20倍。若使第1凸部的高/ 寬亦爲1/ 1〜20/ 1更理想。據此，可高精度的生產細胞培 養容器。 更理想’係在該凹凸樣式所設置之區域進行表面處理。 即’在凸部1 3的上面及側面，和鄰接凸部1 3之間的底面 進行表面處理較理想。作爲表面處理，例如，可進行有機 材料或無機材料之塗膜。有機材料或無機材料可依據目的 -16 - 200804588 適當地選擇習知之材料。又，亦可以紫外線、電子線等活 性能源線處理、或由其他藥品直接將表面變質。 細胞培養容器上的凹凸樣式，可藉由砍酮作爲材料的半 導體加工技術或依據光罩蝕刻法使用鎳製蓋印機的成形技 術形成。例如，以矽酮作爲材料的半導體加工技術時，在 矽酮基板上塗抹光阻，並將光阻曝光，顯像將光阻樣式化。 從此光阻上依據蝕刻矽酮，使矽酮基板樣式化，形成凹凸 模型。而，藉由剝離光阻，可完成具有所欲凹凸樣式之矽 酮製細胞培養容器。當然，細胞培養容器用的基板不限於 矽酮基板，亦可爲玻璃基板或樹脂基板。藉由使用如此半 導體加工技術，可形成精度優良的凹凸樣式。 又，使用鎳製蓋印機的成形技術時，首先，在基板上塗 抹第1光阻層，曝光，更在其上面塗抹第2光阻層，曝光。 藉由總括顯像該第1光阻層及第2光阻層，可在基板上形 成凹凸樣式。而在形成此凹凸樣式的基板上藉由蒸鍍法或 濺鍍法附著導電性膜。並從導電性薄膜的上面藉由電鍍堆 疊金屬而形成金屬構造體。藉由金屬構造體從基板剝離可 形成蓋印機。又藉由使用此蓋印機成形，可將高精度的細 胞培養容液以高生產力製造。此種情況，細胞培養容器係， 例如，由樹脂材料形成。當然，亦可由爲了作成金屬構造 體之構造體的矽酮基板或玻璃基板等形成。更，關於細胞 培養容器的製造方法，並不限定於上述方法。具體上，亦 可由機械切削、或對玻璃的蝕刻處理法等製造細胞培養容 器1 0。據此，可製造大面積/細胞培養株。又，藉由玻璃或 樹脂等透明材料爲細胞培養容器的原料，可容易進行細胞 -17- 200804588 的觀察。 藉由具有上述構造的細胞培養容器1 〇，可顯示在生體內 原來發現之形狀或類似機能更有效率的繁殖及/或分化。 更，可提高培養細胞的生存率。即，藉由在凹部1 4注入細 胞，並供應培養液，可效率好地培養細胞。更，可提供簾 價且容易觀察的細胞培養容器。 又，依據在微細凹凸樣式所形成的空間內進行細胞培 養，可顯示原來在生體內發現之立體形狀，藉由微細凹凸 之立體的區劃，可提高細胞本身所產生的細胞繁殖因子或 分化誘導因子等之生理活性物質的細胞周圍濃度。因此， 可提供效率好之繁殖及/或分化的細胞培養容器。更以透 明樹脂原料製作故可提供簾價且容易觀察的細胞培養容 又，凹凸樣式形成2階之細胞培養容器，對於成爲替代 人造內臟器官之生物反應器亦有效。由於將基板重疊，使 培養液形成環流之空間時，爲了防止培養細胞產生物質的 擴散與側壁直行之方式設置比側壁高的橋樑，使培養液朝 與側壁直行之方向流動，可防止從培養細胞放出二氧化碳 而引起之PH的變化，及培養細胞產生物質的擴散。 此外，在本實施形態，其構成雖說明在第1凸部1 1的上 面設置第2凸部1 2，但本發明並不限於此。例如，亦可在 第1凸部1 1之上面不設置第2凸部12的構成。即，亦可 將高度不同的第1凸部及第2凸部分別設置在不同的位 置。此時，並非2階以上之階級狀的凸部，而是設置高度 不同的2種以上之凸部的構成。 -18- 200804588 發明之實施形態2 有關本發明之實施形態的細胞培養容器構成用第3圖、 第4圖及第5圖加以說明。第3圖係顯示有關本實施形態 之細胞培養容器構成的俯視圖，第4圖係顯示第3圖之虛 線內構成放大的俯視圖。第5圖係第4圖之B — B ’剖視 圖。此外，關於與在實施形態1說明之構成相同的構成其 說明在此省略。 有關本實施形態之細胞培養容器1 0形成與實施形態1相 同的凹凸樣式。因此，關於第1凸部1 1及第2凸部分12 之形狀、尺寸因與實施形態1相同故省略其說明。更，有 關本實施形態之細胞培養容器1 〇，除了在實施形態1說明 的構成之外，在凹部1 4的底面形成凹槽1 7。即，在鄰接之 第1凸部1 1之間，底面設置有凹槽17。凹槽17在鄰接之 第1凸部1 1之間設置複數個。因此，在第1凸部1 1之側 壁15的附近，細胞培養容器1 〇的剖面形狀，係形成3階 的階級狀。凹槽1 7在凹部1 4的底面配置成矩陣狀。 如此，有關本實施形態之細胞培養容器1 〇，在細胞培養 容器1 0凹部1 4的底面形成複數個微細凹槽1 7。凹槽1 7的 寬度，理想上小於培養細胞的相當直徑。更理想，係使凹 槽17寬度爲培養細胞之相當直徑的〇·〇〇1〜〇·9倍。又，凹 槽1 7的深度，理想係0 · 1 # m〜5 0 β m。即，理想上藉由凹 槽17所形成之空間的大小爲培養細胞之相當直徑的 0.001〜0.9倍以下，空間的高度爲m〜50// m。 又，凹凸樣式均可作爲2階或3階以上，凹凸樣式作爲2 階時，需形成爲了設置凹部1 4的第1凸部Η。而藉由在第 -19- 200804588 . 1凸部1 1所形成之凹部1 4的底面配設複數的凹槽1 7。 第1凸部1 1及第2凸部分1 2，可與實施形態1相同的形 狀。即，凹部14及凸部13的尺寸，在實施形態1所顯示 的範圍較理想。亦可在設置凹凸樣式的區域進行表面處 理。又，有關本實施形態之細胞培養容器1 0的製造，可與 實施形態1相同的方法製造。 藉由具有上述構造之細胞培養容器1〇，可顯示原來在生 體內發現之形狀或類似機能，可更有效率的繁殖或/及分 化。更可提供簾價且容易觀察的細胞培養容器。因此，可 獲得與實施形態1同樣的效果。 其次，有關本發明之實施形態的細胞培養容器之實施例 用第6圖至第9圖加以說明。 〔實施例1〕 有關實施例1之細胞培養容器的形狀用第6圖加以說 明。第6圖係顯示本實施例之細胞培養容器形狀的立體圖。 在第6圖，2階的階級狀凸部13設置排成一列。凸部13 分別延深入方向設置。鄰接凸部1 3之間的凹部1 4成爲培 養液流動的凹溝。鄰接之凹部1 4在凸部1 3的外側互相連 通。在此，鄰接凸部1 3之間的凹部1 4成爲培養細胞的培 養基S。藉由如第6圖所示之形狀，可確保飛躍的培養基。 此外，在細胞培養容器的表面上，設配置複數凸部1 3的方 向爲寬方向，與其正交的方向爲深入方向。在此，第1凸 部11及設置在第1凸部11上面的第2凸部分12之深入方 向的大小大略一致。 如第6圖所示，細胞培養容器1 〇之深入方向的大小爲A， -20- 200804588 細胞培養容器之寬爲B，細胞培養容器1 〇之厚（高）度爲 C，第1凸部1 1之深入方向的大小爲d，第1凸部1 1之寬 爲e，第1凸部1 1之高度爲f ’鄰接之第1凸部1 1之間的 間隔爲g。在此，有關本實施例之細胞培養容器1 〇的A、B、 C、d、e、f及g之適當値顯示如第1表。 〔第1表〕 尺寸（// m) 培養試驗 培養株製造、組織培養 A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100-10,000 100-200,000 d 1-150,000 1-2,000,000 e 1 〜1,500 1-2,000 f 1〜1,500 1-2,000 g 2〜3,000 2-3,000 f/e 1/1 〜20/1 1/1 〜20/1 在第1表記載適合細胞培養試驗之尺寸，及適合培養株 製造、組織培養的尺寸。依據如第1表所示之尺寸範圔設 定凸部1 3及細胞培養容器1 〇的大小，可獲得適合培養的 細胞培養容器，或培養株的製造及適合組織培養的容器。 在此，f/e爲1/1〜20/1。即，第1凸部11之高度爲寬度 的1〜20倍。更，第2凸部分12之深入方向的大小、寬及 -21 - 200804588 高度，和鄰接之第2凸部1 2之間隔設定與d〜g同樣的範圍。 此外，第1凸部丨丨設定成比第2凸部分12小。即，第2 凸部分1 2之深入方向的大小及寬度可分別設定大於第1凸 部1 1之深入方向的大小及寬度。 〔實施例2〕 有關實施例2之細胞培養容器的形狀用第7圖加以說 明。第7圖係顯示有關本實施例之細胞培養容器形狀的立 體圖。在第7圖，2階的階級狀凸部1 3配列成矩陣狀。即， 在本實施例之細胞培養容器1 0具有在第1凸部1 1的上面 配置比第1凸部1 1小的第2凸部分12之構成。由此第1 凸部1 1及第2凸部分1 2所構成的凸部1 3配列成矩陣狀。 鄰接之凹部14，在第1凸部1 1的上側互相連通。在凸部 1 3與凸部1 3之間的凹部1 4培養細胞。即，凹部1 4成爲細 胞的培養基。藉由如第7圖所顯示之形狀，可獲得飛躍的 培養基。 如第7圖所示，細胞培養容器1 〇之深入方向的大小爲A， 細胞培養容器之寬爲B，細胞培養容器1 〇之厚（高）度爲 C，第1凸部11之深入方向的大小爲d ’第1凸部Π之寬 爲e，第1凸部1 1之高度爲f ’鄰接之第1凸部1 1之間的 間隔爲g。在此，有關本實施例之細胞培養容器1 0的A、B、 C、d、e、f及g之適當値顯示如第2表。 •11- 200804588 〔第2表〕 尺寸< { β m) 培養試驗 __ 培養株製造、組織培養 A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100-10,000 100-20,000 d 1-150,000 1-2,000,000 e 1-1,500 1-2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1-20/1 1/1 〜20/1 在第2表記載適合細胞培養試驗之尺寸’及適合培養株 製造、組織培養的尺寸。依據如桌2表所不之尺寸範圍設 定凸部13及細胞培養容器丨〇的大小’可獲得適合培養的 細胞培養容器，或培養株的製造及適合組織培養的容器。 在此，f/e爲1/1〜20/!。即’第1凸部11之局度爲寬度 的1〜2 0倍。更，第2凸部分1 2之涂入方向的大小、寬及-高度，和鄰接之第2凸部1 2之間隔設定與d〜g同樣的範圍。 此外，第1凸部1 1設定成比第2凸部分12小。即，第2 凸部分1 2之深入方向的大小及寬度可分別設定大於第1凸 部1 1之深入方向的大小及寬度。 〔實施例3〕 有關實施例3之細胞培養容器的形狀用第8圖加以說 明。第8圖係顯示有關本實施例之細胞培養容器形狀的立 體圖。在本實施例以替代人造內臟器官爲目的之生物反應 器等，可使培養液循環之模型加以說明。在第8圖，第1 凸部1 1與比第1凸部1 1高之第2凸部分1 2分別設置在不 -23- 200804588 同的位置。即，高度相異之第1凸部n與第2凸部分12 分別設置。具體上，第2凸部1 2分別設置於細胞培養容器 1 0的兩端’其間設置有複數的第1凸部丨丨。第2凸部1 2， 係沿著細胞培養容器1 〇的端緣設置。設置在細胞培養容器 10之兩端的第2凸部12,高於設置在中央之第1凸部11。 即’第2凸部1 2成爲比第1凸部1 1之側壁1 6高的橋樑。 據此’可維持PH，並防止從細胞產生物質的擴散。 第1凸部1 1，係沿著第2凸部分1 2設置方向排成一列。 而各個第1凸部11，係與第2凸部分12設置方向成正交方 向設置。凸部1 3分別延深入方向設置。鄰接凸部1 3之間 的凹部1 4成爲培養液流動的凹溝。據此，培養液流動於與 第2凸部分1 2正交方向。即，透過細胞培養容器1 〇的中 央部培養液從一端流向另一端。據此，可防止產生物質的 擴散。又，鄰接之凹部1 4在第1凸部1 1的上側互相連通。 據此，可使培養液循環。 如第8圖所示，細胞培養容器1 〇之深入方向的大小爲A, 細胞培養容器10之寬爲B，細胞培養容器10之厚（高） 度爲C，第1凸部1 1之深入方向的大小爲d ’第1凸部1 1 之寬爲e，第1凸部11之高度爲f，鄰接之第1凸部11之 間的間隔爲g。在此，有關本實施例之細胞培養容器1 〇的 A、B、C、d、e、f及g之適當値顯示如第3表。 -24- 200804588 〔第3表〕 尺寸< (μ m) 培養試驗 培養株製造、組織培養 A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100 〜100,000 100-200,000 d 1 〜150,000 1 〜2,000,000 e 1-1,500 1-2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1-20/1 1/1 〜20/1 在第3表記載適合細胞培養試驗之尺寸，及適合培養株 製造、組織培養的尺寸。依據如第3表所示之尺寸範圍設 定凸部1 3及細胞培養容器1 0的大小，可獲得適合培養的 細胞培養容器，或培養株的製造及適合組織培養的容器。 在此，f / e爲1/1〜20/1。即’第1凸部11之局度爲寬度 的1〜2 0倍。更，第2凸部分12之深入方向的大小、寬及 高度，和鄰接之第2凸部1 2之間隔設定與d~g同樣的範圍。 〔實施例4〕 有關實施例4之細胞培養容器的形狀用第9圖加以說 明。第9圖係顯示有關本實施例之細胞培養容器形狀的立 體圖。在本實施例以替代人造內臟器官爲目的之生物反應 器等，可使培養液循環之模型加以說明。在本實施例對應 於實施形態2之細胞培養容器1 0的構成加以說明。如第9 圖所示，在細胞培養容器10，藉由第1凸部1 1形成凹部 14的底面設置複數個凹槽17。 在細胞培養容器1 0的兩端，分別設置第1凸部1 1 ’即， -25- 200804588 2個第1凸部1 1分別設置於沿著細胞培養容器1 0的端邊。 而在鄰接之第1凸部11之間，複數個凹槽17配置成矩陣 狀。第1凸部1 1形成高於凹槽1 7之側壁的橋樑。 如第9圖所示，細胞培養容器1 0之深入方向的大小爲A， 細胞培養容器1 0之寬爲B，細胞培養容器1 0之厚（高） 度爲C，凹槽17之深入方向的大小爲d，凹槽17之寬爲e， 凹槽1 7之高度爲f，鄰接之凹槽1 7間的間隔爲g。在此， 有關本實施例之細胞培養容器1 0的A、B、C、d、e、f及 g之適當値顯示如第4表。 〔第4表〕 尺寸< { U m) 培養試驗 培養株製造、組織培養 A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000〜2,000,000 C 100 〜100,000 100-200,000 d 1-150,000 1-2,000,000 e 1-1,500 1-2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1 〜20/1 1/1 〜20/1 在第4表記載適合細胞培養試驗之尺寸，及適合培養株 製造、組織培養的尺寸。依據如第4表所示之尺寸範圍設 定凸部1 3及細胞培養容器丨〇的大小，可獲得適合培養的 細胞培養容器’或培養株的製造及適合組織培養的容器。 在此，理想上f / e爲1 / 1〜2 0 / 1。即，凹槽1 7之深度爲 寬度的1〜20倍。更，第1凸部u之深入方向的大小、寬 及咼度’和鄰接之第1凸部1 1之間隔設定與d〜g同樣的範 -26- 200804588 圍。 【圖式簡單說明】 第1圖係顯示有關本發明實施形態丨之細胞培養容器構 成的俯視圖。 第2圖係顯示有關本發明實施形態1之細胞培養容器構 成的剖視圖。 第3圖係顯示有關本實施形態2之細胞培養容器構成的 俯視圖。 第4圖係顯不有關本發明實施形態2之細胞培養容器構 成的放大俯視圖。 第5圖係顯示有關本發明實施形態2之細胞培養容器構 成的剖視圖。 第6圖係顯示有關本發明實施例丨之細胞培養容器構成 的斜視圖。 第7圖係顯示有關本發明實施例2之細胞培養容器構成 的斜視圖。 第8圖係顯示有關本發明實施例3之細胞培養容器構成 的斜視圖。 第9圖係顯示有關本發明實施例4之細胞培養容器構成 的斜視圖。 【主要元件符號說明】 10 胞培養容器 11 第1凸部 12 第2凸部 13 凸部 -27- 200804588 14 凹部 15 第1 凸部的側壁 16 第2 凸部的側壁 17 凹槽 -28200804588 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD The present invention relates to a cell culture container and a method of using the same. [Prior Art] Cell death can be largely apoptotic and bad: Apoptosis means that the cells that make up a multi-celled organism are actively responsible for cell suicide. Most of the cancerous cells (and other cells) in the body are prevented by the growth of most of the tumors through apoptosis. The process will die at a predetermined time and in the determined field of the planned cell, and this is a morphological change of the organism, which occurs due to the structure of apoptosis. For example, the disappearance of sputum is caused by apoptosis. The mites are recorded in the hair. In addition, in the immune system, it also plays an important role in the removal of cells. Conversely, due to poor blood, trauma, and other cell death caused by fineness, called necrosis (necr 〇sis), the source of death is from the Greek word "apo - (departure)", with "(dead leaves, etc. from the tree) The necrosis of "falling off" means that part of the biological tissue has died. Only cells that form part of the body die. It was originally broken, chemical damage, and decreased blood flow. The person is especially called infarction. Similarly, the cells die, but the cells like the container die (n e c r 〇 s i s). One type of so-called cell death method is the removal of an internal abnormality that is managed and regulated. According to this, it is customary that the cell death occurs in the occurrence of the organism (the motive force of the being, and the tail of the frog) The deterioration of the internal and external environment caused by the apoptosis of the self-antigen reaction is different from this. ") and "ptosis". The meaning is different from the usual death, due to infection, physical reduction in blood flow. The abnormal cells of the mucosal epithelium that cause the blood cells, the skin, and the digested 200804588 tube, and the disorder that the tissue is continuously replenished, and the histological abnormalities are not called necrosis. The necrosis is finally removed by the living body's immune system, and the defective part is removed. It is compensated by woven regeneration or line maintenance. In order to inspect and test such cells, it is usually necessary to separate and culture from the group. The method of separating cells from tissues and using them for examination is indispensable in the field of biotechnology. It is used in diseases, pathological clinics, exploration and destruction of new drugs, or animal inspections, plant inspections, and environmental pollutants. Although the cells are directly used for the test, most of the culture methods are cultured in a culture tray or a test tube. Various examinations are carried out here. These analyses usually set a uniform culture system and change the amount to be evaluated. The effect is observed in terms of concentration, etc. Therefore, the culture for culture is formed to be uniform and uniform. The culture container is generally a scale (cell culture tray). As a general user of the culture tray (schale), or a 6-well plate ( 6well plate), 12-well plate, 48-well plate, etc. (Patent Document 1). From the recent miniaturization, the bottom of the 384-hole plate with a small diameter and a large number of plates is flat. In the medical and biotechnology fields, a cell culture plate (schale), a hole, or the like, which is used for the purpose of cultivating a woven culture, is used as a culture surface. However, tissue cells are used. When the culture is carried out in the form of a flat plate at the bottom, the cells are thinly stretched to have a non-directional shape, and the cells are examined and tested by the original tissue. The medicinal test of the medicinal effect, etc. The container of the cultivar is also cultured in a cell culture system, and the 96-well plate is further opened. The culture test and the plate are performed on the plate. It is impossible to show the shape or function that can be seen in the living body. For example, fat cell precursor culture cells are cultured in a flat plate or culture vessel, or fat cells that cannot be differentiated into a target when differentiation is induced. The problem of the shape. In addition, there is a problem that the hepatocytes cannot form a spherical mass-shaped cell mass. Therefore, in the case of a flat plate, since the cell growth or cell differentiation which is effective for the purpose is trapped, there is a problem. A method of immobilizing a cytokine (cyt 〇kine) or an extracellular matrix on a flat surface or a method using a special device (Patent Document 2, Patent Document 3). However, the method of fixing the cell growth factor or the extracellular matrix in the form of a plate has an efficiency in the initial stage, and it is not possible to maintain it continuously or because the immobilization method is cumbersome and cannot be stably manufactured, and it is costly. Further, the method using a special device differs depending on the result of the cell type, and it is impossible to estimate stable efficiency, or the use of a large erected device results in poor operability and cost. Further, in the culture test using a cell culture plate (scha 1 e) or a well plate or tissue culture, it is known that the activity of the cultured cells may be due to the change in the pH of carbon dioxide or the like discharged from the cultured cells. Great impact. Therefore, culture is carried out for a long period of time, and the culture medium is kept in a regular manner (e.g., once every 2 days) to maintain the activity of the cultured cells. More in the latest research examples, there are proposals to create new human internal organs replacement models that often allow fresh culture fluids to circulate bioreactors. However, in order to suppress the necrosis of necrosis such as nerve cells or myocardium, skeletal muscles, etc., or the cells of the regenerative tissues of the skin and the mucosal epithelium of the digestive tract, the factor of death is not clear, but the current situation is only replaced with fresh The culture solution of 200804588 still could not achieve cell death inhibition. In particular, it is difficult to culture cultured mouse cardiomyocytes, nerve cells, corneal cells, etc., and even if fresh culture medium is replaced, the survival rate of the number of cells initially dispersed (arranged) is only 20 to 40%. In recent years, the use of semiconductor processing technology using fluorenone as a material, or the development of a nickel stamping machine (original disk) based on the reticle remnant method, has been attempted from the public, micron, and The level of rice is fine. However, the cell culture porous disk or the like produced by the microfabrication technique can be used for micro-chemicalization and integration of the sample, but there is no suggestion of a cell species which is difficult to culture. [Patent Document 1] Japanese Patent Laid-Open Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. The problem to be solved] In the previous cell culture container, the cell adopts a thin and stretched shape, and cannot display the shape or function originally found in the living body, and the cell or shape cell that is originally found in the living body is efficiently used. Efficient breeding or / and differentiation problems. The problem to be solved by the present invention is to provide a cell culture container which can be efficiently and efficiently displayed in a living body and which can be more efficiently propagated or/and differentiated in a shape or function, and a cell culture method using the same . [Means for Solving the Problem] The cell culture container according to the first aspect of the present invention is a cell culture container having a concave-convex type 200804588 type, and the size of the concave portion formed by the unevenness of the uneven pattern is a cultured cell. A considerable diameter of 1. 〇 times to 40 times. According to this, the efficiency can be improved, and the shape or function originally found can be more efficiently propagated and/or differentiated in the living body. A cell culture container according to a second aspect of the present invention is characterized in that the concave portion is formed based on the second-order or more irregularities in the cell culture container. According to this, the efficiency can be improved, and the shape or function originally found can be more efficiently propagated and/or differentiated in the living body. In the cell culture container according to the third aspect of the present invention, the size of the bottom portion of the concave portion formed by the unevenness of the second or higher order in the cell culture container is 1.5 to 10 times the diameter of the cultured cell. . According to this, it is efficient and shows that the shape or function originally found can be more efficiently propagated and/or differentiated in the living body. In the cell culture container according to the fourth aspect of the present invention, at least one of the concave portions formed by at least one of the irregularities of the second order or more is connected to at least one of the adjacent concave portions. Its characteristics. According to this, the efficiency can be improved, and the shape or function originally found can be more efficiently propagated and/or differentiated in the living body. According to a fifth aspect of the present invention, in the cell culture container, the height of the lowermost portion of the concave portion formed by the unevenness of the second or higher order is 1 # m to 1 0 0 // m. Its characteristics. According to this, it is efficient and shows that the shape or function originally found can be more efficiently propagated and/or differentiated in the living body. The cell culture container according to the sixth aspect of the present invention is characterized in that, in the cell culture container, the bottom portion of the concave portion formed based on the unevenness has a plurality of concaves 80,804,588 grooves having a size which is a considerable diameter of the cultured cells. 1 to 〇. 9 times. According to this, the efficiency can be improved, and the shape originally found can be more efficiently propagated and/or differentiated in the living body. A cell culture container according to a seventh aspect of the present invention is characterized in that the height of the groove is up to 50/m in the container, so that the shape or the machine originally found in the living body is displayed. Efficiency of reproduction or / and differentiation. The cell culture container according to the eighth aspect of the present invention is characterized in that the height of the concave portion formed by the unevenness is 1//m3 m in the container. According to this, the efficiency can be improved, and the original shape or function can be reproduced in the living body to more efficiently propagate and/or differentiate. A cell culture container according to a ninth aspect of the present invention is characterized in that, in the container, the surface is characterized in a region where the concavo-convex pattern is provided. According to this, the efficiency is good, and the function originally found in the living body can be more efficiently propagated and/or differentiated. The cell culture container according to the tenth aspect of the present invention is characterized in that the container material is a transparent material. According to this, observation is possible. In the cell culture method according to the first aspect of the present invention, the cells are cultured by injecting cells into the concave portion of the cell culture vessel. It is efficient and shows the reproduction or/and differentiation of the shape or function that was originally found in the living body. [Effects of the Invention] According to the present invention, it is possible to provide an efficient and efficient reproduction of the shape or function originally found, and/or its characteristic or mechanical cell culture. According to the energy cell culture: 200 // The cell culture found is easy to be inserted into the shape or cell culture, and the cell culture medium can be more effectively differentiated in vivo. -08 200804588 Cell culture container and cell culture method using the same . [Embodiment] [Best Embodiment of the Invention] When culture of tissue cells is carried out on a cell culture plate (schale or well plate) currently on the market, the cultured cells have a shape that is thin and stretched in a non-directional direction. As a method for confirming cell activity, the researcher attempts to compare the measurement data of the living tissue and the cells cultured in the culture plate by changing the pH of the old waste or by passing the carbon dioxide discharged through the electrochemical sensor. The data were measured, but the current status of the data in the living tissue was not displayed in the culture plate. Therefore, culture on a cell culture plate marketed on the market is judged to be that the cultured cells do not exhibit the functions held in the living body. Therefore, a micro-container style suitable for propagating tissue cells is formed on the culture tray, and the cells are cultured in the micro-container style, so that the three-dimensional propagation cells can be smoothly found to be able to smoothly discover the functions held in the living body. . The subject is not to be said, the efficient reproduction and/or differentiation of its cells. According to the culture method using the cell culture container according to the present invention, by providing the fine concavo-convex pattern, it is possible to have the same three-dimensional growth as in the living body, and the cell reproduction factor produced by the three-dimensionally-divided cells formed by the fine concavities and convexities and/or A physiologically active substance such as a differentiation inducing factor can be cultured at a similar concentration to that in the living body, so that it is possible to mimic the environment around the cells in the living body. The fine concavo-convex pattern has, for example, a plurality of side walls, and a plurality of spaces for arranging the cultured cells formed by the side walls, and an opening portion for forming a plurality of spaces can be realized by providing openings in the side walls. Since there are a plurality of side walls, a plurality of spaces are created, and the size of the space can be set in accordance with the required use. -11- 200804588 The size of the side wall, the space, and the opening are set according to the cell type to be cultured. It is assumed that a variety of culture systems may be applied. The opening is a structure in which a space (a recess) formed by the side walls can communicate with each other as an opening. By the size of the space formed by the side walls, it is necessary to have the most appropriate range for the purpose of culturing the cells. If the space formed by the side wall is too large, the cells are stretched as thin as the plate, and the three-dimensional structure cannot be displayed. However, if the space is too small, the cells cannot enter the space. Therefore, the size of the space, depending on the type of cells to be cultured, can be accommodated in a single or multiple range. As a result of intensive research by the inventors of the present invention, it has been found that the shape of a specific fine pattern can be improved by the prevention of diffusion of a cell-producing substance, and it is found that the survival rate of the cultured cells can be greatly improved. During the culture process, cells produce fibronectin, laminin, collagen, elastin and the like. Although the pH change caused by carbon dioxide excretion is the main cause of the decrease in activity when culturing cells, the reverse generation of such substances can be used for matrix materials and the like, and is also indispensable for the subsequent, differentiation/reproduction of the surface of the cell substrate. material. The inventors of the present invention have formed a plurality of fine patterns in a cell culture container, and by culturing cells in the lower portion of the side wall of the pattern, it is possible to successfully prevent the spread of the cell-producing substance and improve the survival rate of the cells. It prevents the spread of substances produced by cells and increases the survival rate of cells. In the future, it is expected to increase the productivity of cell culture plants. Therefore, in the fine pattern of the present invention, how to ensure the length of the lower portion of the side wall in the culture area is -12-200804588. The lower width between the side walls, the minimum size of a single cell is 4 to 5 // m, so that, for example, 2 // m or more is desired. The width of the side wall is desirably as small as possible in order to secure the length of the lower portion of the side wall in the culture area, but it is expected to be 1 μm or more from an industrially reproducible point of view. The height of the side wall is, for example, 1 / m or more from the viewpoint of suppressing the diffusion of the generated substance. The aspect ratio of the protrusions at all levels is designed to prevent the diffusion of substances. Although it is expected to be as high as possible, from the viewpoint of industrial technology reproducibility, the height/width ratio is expected to be selected from 1/1 to 20/1. . Further, the cell culture container according to the present embodiment is also effective as a bioreactor or the like which is an alternative to an artificial internal organ. When the substrate is overlapped and the culture solution is formed into a space for circulation, a bridge having a height higher than that of the side wall is provided in order to prevent the diffusion of the substance in the cultured cells and the side wall is straight, and the culture liquid is caused to flow in a direction perpendicular to the side wall, thereby preventing the cultured cells from being cultured. The change in pH caused by the release of carbon dioxide, and the diffusion of substances produced by the cultured cells. Hereinafter, the cell culture container of the aspect of the present invention will be described in detail based on the drawings. EMBODIMENT OF THE INVENTION The specific shape of the cell culture container according to the first embodiment of the present invention will be described below with reference to Figs. 1 and 2 . Fig. 1 is a plan view showing a configuration of a cell culture container according to the embodiment, and Fig. 2 is a cross-sectional view taken along line A-A' of Fig. 1. Of course, the configuration shown in Figs. 1 and 2 is an example of the cell culture container of the present invention, and the configuration is not limited. The cell culture vessel is formed into a concave and convex pattern. The embossed pattern forms a class of 2 • 13 - 200804588. Therefore, in the culture surface of the cell culture vessel, the second-order convex portion 13 is formed. In other words, the second convex portion 12 having a rectangular parallelepiped shape forming the first convex portion 1 1 in a lattice shape is formed in a matrix shape. The space formed by the first convex portion 1 1 and the second convex portion 1 2 is the concave portion 14. That is, the space between the adjacent first convex portions n and the space between the second convex portions 12 become the concave portion 14. The side wall 15 of the first convex portion 1 1 and the side wall of the adjacent first convex portion 1 1 and the side wall 16 of the second convex portion 1 2 and the side wall 16 of the adjacent second convex portion 1 2 The space between them forms a recess 14 for culturing the cells. The side wall 15 of the first convex portion 1 1 and the side wall 16 of the second convex portion 1 2 are substantially perpendicular to the bottom surface. Therefore, the recessed portion 14 has a stepped shape of two-order unevenness. The first convex portion 1 1 is arranged in a lattice shape of four sides enclosing the rectangular concave portion 14 as shown in Fig. 1 . The second convex portion 1 2 is disposed in an island shape on the upper surface of the first convex portion 1 1 between the adjacent concave portions 14. The second convex portion 1 2 is provided on each of the four sides of the rectangular recessed portion 14 . Further, the shape of the concave portion 14 is not limited to a rectangular shape, and may be a polygonal shape, a circular shape, a rounded shape, or the like. Further, the ideal concavo-convex pattern is two or more steps, and the concave portion 14 is formed by two or more steps. That is, the recessed portion 14 may have a step shape of two or more steps. Of course, the recess 14 can also be 1st order, and 3rd order or more can also be used. According to this, it can be a shape suitable for cultivation. The size (width, depth) of the concave portion 14 formed according to the concave-convex pattern is preferably from 1 to 40 times the diameter of the cultured cells. That is, the interval between the adjacent convex portions 1 3 is preferably 1 to 10 times the diameter of the cultured cells. In other words, the width of the concave portion 14 is preferably 1.0 to 40 times the equivalent diameter of the cultured cells. Further, since the minimum size of the single cells is 4 to 5 // -14 to 200804588 m, the width between the side walls 15 of the adjacent first convex portions 1 1 is preferably, for example, 2 // m or more. Further, the size (width, depth) of the bottom of the concave portion 14 is preferably 1.5 to 10 times the equivalent diameter of the cultured cells. That is, the width of the space formed by the first convex portion 1 1 is preferably 1.5 times to 10 times the diameter of the cultured cells. In other words, the interval between the formation of the adjacent first convex portions 11 is preferably 1.5 to 1 times the diameter of the cultured cells. According to this, it can be a size suitable for cultivation. Further, the second-order recessed portion 14 is preferably connected to at least one or more of the adjacent second-order recessed portions 14 . Here, the upper side of the first convex portion 1 1 is in communication with the adjacent second-order concave portion 14. In other words, the second-order recessed portion 14 adjacent to the space between the side walls 16 of the adjacent second convex portion 12 is communicated. Specifically, the convex portion 1 1 of the younger brother 1 is placed in a lattice shape, and the island-shaped second convex portion 1 2 is scattered thereon. According to this, since the space of the second convex portion 1 2 is not completely separated, the second portion of the concave portion 14 adjacent to the space on the first convex portion 1 1 is communicated. Further, when the concave portion 14 is of the second order or more, the space formed in the first order or more is preferably at least in communication with the adjacent concave portion 14 . In other words, it is preferable that at least one of the concave portions of the second-order or more irregularities is formed to communicate with the adjacent other concave portions 1 4 . At this time, the convex portion 14 is formed on the lower convex portion by the uppermost convex portion, and the concave portions 14 can be communicated with each other. The height at least one step of forming the concave portion 14 is in communication with the adjacent concave portion 14 . In this manner, it is preferable to provide the openings in the concavo-convex pattern and to connect the recesses to each other. Needless to say, the concave portion may be connected to one or more other concave portions, and accordingly, it is possible to have a shape suitable for cultivation. Further, the height of the concave portion is preferably 1 to 200 // m. That is, the sum of the height of the first convex portion 1 1 and the height of the second convex portion 1 2 is preferably 1 to 200 μm. According to this, it can be a size suitable for cultivation. • 15-200804588 Further, the height of the first convex portion 1 1 is preferably 1 to 100 μm. That is, the height of the lowermost step of the stepped recess 14 is preferably 1 to 1 00 // m. Accordingly, the height of the bottom portion of the concave portion 14 is 1 to 100 / / m, which is suitable for the size of the culture. The width of the convex portion 13 is desirably as small as possible in order to ensure the length of the side wall in the culture area. However, from the viewpoint of industrial technology, for example, it is desirable that it is l//m or more. Specifically, the width of the second convex portion 12 is set to be l//m or more. In other words, the interval between the two side walls 16 constituting the second convex portion 12 is l//m or more. Further, the width of the first convex portion 11 may be larger than that of the second convex portion 12. In other words, since the second convex portion 12 is on the upper surface of the first convex portion 1 1 , it is not required to be exposed than the first convex portion 11 . The height of the concave portion 14 is, for example, expected to be l//m or more from the viewpoint of suppressing the diffusion of the generated substance. That is, the sum of the heights of the first convex portion 11 and the second convex portion 12 is preferably 1 / 4 m or more. More preferably, the height of the recess 14 is below 200 /z m. The aspect ratio of the second convex portion 1 2 is desirably as high as possible for the purpose of preventing the diffusion of the substance, but it is desirable from the viewpoint of industrial technology that the height/width ratio is selected from 1/1 to 20/1. . That is, it is desirable that the height of the second convex portion 12 is 1 to 20 times the width of the second convex portion 12. It is more preferable that the height/width of the first convex portion is also 1/1 to 20/1. According to this, the cell culture container can be produced with high precision. More preferably, the surface treatment is performed in the area where the concavo-convex pattern is provided. That is, it is preferable to perform surface treatment on the upper surface and the side surface of the convex portion 13 and the bottom surface between the adjacent convex portions 13 . As the surface treatment, for example, a coating film of an organic material or an inorganic material can be carried out. The organic material or the inorganic material can be appropriately selected according to the purpose -16 - 200804588. Further, the surface of the active material such as ultraviolet rays or electronic wires may be treated, or the surface may be directly deteriorated by other drugs. The concavo-convex pattern on the cell culture vessel can be formed by a semiconductor processing technique using chopping ketone as a material or a forming technique using a nickel stamping machine by a mask etching method. For example, in a semiconductor processing technique using an fluorenone as a material, a photoresist is applied on a fluorenone substrate, and the photoresist is exposed, and the photoresist is patterned by development. From this photoresist, the fluorenone substrate was patterned according to the etching of the fluorenone to form a concave-convex model. Further, by peeling off the photoresist, a cell culture container made of an anthrone having a desired concavo-convex pattern can be obtained. Of course, the substrate for the cell culture vessel is not limited to the fluorene ketone substrate, and may be a glass substrate or a resin substrate. By using such a semiconductor processing technique, a concave-convex pattern excellent in precision can be formed. Further, in the molding technique using a nickel stamping machine, first, a first photoresist layer is applied onto a substrate, exposed, and a second photoresist layer is applied thereon to expose the film. By collectively developing the first photoresist layer and the second photoresist layer, a concave-convex pattern can be formed on the substrate. On the substrate on which the concavo-convex pattern is formed, a conductive film is attached by a vapor deposition method or a sputtering method. A metal structure is formed by plating a metal from the upper surface of the conductive film. The stamping machine can be formed by peeling off the substrate from the metal structure. Further, by using this stamping machine, high-precision cell culture liquid can be manufactured with high productivity. In this case, the cell culture container is formed, for example, of a resin material. Of course, it may be formed of an fluorenone substrate, a glass substrate or the like for forming a structure of a metal structure. Further, the method for producing the cell culture container is not limited to the above method. Specifically, the cell culture vessel 10 can also be produced by mechanical cutting, etching treatment of glass, or the like. According to this, a large area/cell culture strain can be produced. Further, by using a transparent material such as glass or resin as a raw material for the cell culture vessel, observation of the cells -17-200804588 can be easily performed. By the cell culture vessel 1 having the above configuration, the shape or the like originally found in the living body can be more efficiently propagated and/or differentiated. Moreover, the survival rate of the cultured cells can be improved. Namely, by injecting cells in the concave portion 14 and supplying the culture solution, the cells can be efficiently cultured. Further, a cell culture container which is dratable and easy to observe can be provided. Further, by performing cell culture in a space formed by the fine concavo-convex pattern, the three-dimensional shape originally found in the living body can be displayed, and the cell reproduction factor or differentiation inducing factor produced by the cell itself can be enhanced by the three-dimensional division of the fine concavities and convexities. The concentration around the cells of the physiologically active substance. Therefore, a cell culture vessel which is efficient in propagation and/or differentiation can be provided. Further, it is made of a transparent resin material, so that it can provide a cell culture capacity which is easy to observe, and a cell culture container having a second-order concave-convex pattern is also effective as a bioreactor for replacing an artificial internal organ. When the substrate is overlapped and the culture solution is formed into a space for circulation, a bridge having a height higher than that of the side wall is provided in order to prevent the diffusion of the material from the cultured cells and the side wall is straight, and the culture solution is prevented from flowing in the direction of the side wall, thereby preventing the cultured cells from flowing. The change in pH caused by the release of carbon dioxide, and the diffusion of substances produced by the cultured cells. Further, in the present embodiment, the configuration is such that the second convex portion 1 2 is provided on the upper surface of the first convex portion 1 1 , but the present invention is not limited thereto. For example, the second convex portion 12 may not be provided on the upper surface of the first convex portion 1 1 . In other words, the first convex portion and the second convex portion having different heights may be provided at different positions. In this case, it is not a stepped portion of the second-order or more, but a configuration in which two or more kinds of convex portions having different heights are provided. -18- 200804588 EMBODIMENT 2 The cell culture container structure according to the embodiment of the present invention will be described with reference to Figs. 3, 4, and 5. Fig. 3 is a plan view showing the configuration of the cell culture container according to the embodiment, and Fig. 4 is a plan view showing an enlarged view of the inside of the dotted line in Fig. 3. Fig. 5 is a cross-sectional view taken along line B - B' of Fig. 4. Incidentally, the same configurations as those described in the first embodiment will be omitted. The cell culture vessel 10 of the present embodiment has the same concavo-convex pattern as in the first embodiment. Therefore, the shapes and dimensions of the first convex portion 1 1 and the second convex portion 12 are the same as those of the first embodiment, and thus the description thereof will be omitted. Further, in the cell culture container 1 of the present embodiment, a groove 17 is formed on the bottom surface of the concave portion 14 in addition to the configuration described in the first embodiment. That is, the groove 17 is provided on the bottom surface between the adjacent first convex portions 1 1 . The recess 17 is provided in plurality between the adjacent first projections 1 1 . Therefore, in the vicinity of the side wall 15 of the first convex portion 1 1 , the cross-sectional shape of the cell culture vessel 1 is formed into a three-stage shape. The grooves 17 are arranged in a matrix on the bottom surface of the recess 14 . As described above, in the cell culture container 1 of the present embodiment, a plurality of fine grooves 17 are formed on the bottom surface of the concave portion 14 of the cell culture container 10. The width of the groove 17 is ideally smaller than the equivalent diameter of the cultured cells. More preferably, the width of the groove 17 is 〇·〇〇1 to 9·9 times the diameter of the cultured cells. Further, the depth of the recess 17 is preferably 0 · 1 # m to 5 0 β m. That is, the size of the space formed by the recess 17 is preferably 0.001 to 0.9 times the diameter of the cultured cells, and the height of the space is m 50 50 / m. Further, when the uneven pattern is used as the second order or the third order or more, and the uneven pattern is the second order, the first convex portion 为了 in order to provide the concave portion 14 is formed. Further, a plurality of grooves 17 are provided on the bottom surface of the concave portion 14 formed by the convex portion 11 of the -19-200804588. The first convex portion 1 1 and the second convex portion 1 2 can be formed in the same shape as in the first embodiment. That is, the size of the concave portion 14 and the convex portion 13 is preferably in the range shown in the first embodiment. Surface treatment can also be performed in the area where the bump pattern is set. Further, the production of the cell culture vessel 10 of the present embodiment can be produced in the same manner as in the first embodiment. By the cell culture container having the above configuration, the shape or the like which is originally found in the living body can be displayed, and the reproduction or/and differentiation can be performed more efficiently. It is also possible to provide a cell culture container which is priced and easy to observe. Therefore, the same effects as those of the first embodiment can be obtained. Next, an embodiment of a cell culture vessel according to an embodiment of the present invention will be described with reference to Figs. 6 to 9 . [Example 1] The shape of the cell culture vessel according to Example 1 will be described with reference to Fig. 6. Fig. 6 is a perspective view showing the shape of the cell culture container of the present embodiment. In Fig. 6, the second-order stepped projections 13 are arranged in a line. The convex portions 13 are respectively extended in the depth direction. The concave portion 14 between the adjacent convex portions 1 3 serves as a concave groove through which the culture liquid flows. Adjacent recesses 14 are interconnected on the outside of the projections 13. Here, the concave portion 14 between the adjacent convex portions 1 3 serves as a medium S for culturing cells. The medium of the leap can be ensured by the shape as shown in Fig. 6. Further, on the surface of the cell culture container, the direction in which the plurality of convex portions 13 are disposed is the width direction, and the direction orthogonal thereto is the deep direction. Here, the size of the first convex portion 11 and the second convex portion 12 provided on the upper surface of the first convex portion 11 substantially coincide. As shown in Fig. 6, the size of the cell culture vessel 1 in the depth direction is A, -20-200804588, the width of the cell culture vessel is B, and the cell culture vessel 1 is thick (high) C, the first convex portion The size of the depth direction of 1 1 is d, the width of the first convex portion 1 1 is e, and the interval between the first convex portions 1 1 having a height f of the first convex portion 11 is g. Here, the appropriate enthalpy of A, B, C, d, e, f, and g of the cell culture vessel 1 of the present embodiment is shown in Table 1. [Table 1] Size (// m) Culture test culture Plant manufacturing, tissue culture A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100-10,000 100-200,000 d 1-150,000 1-2,000,000 e 1 ~ 1,500 1-2,000 f 1~1,500 1-2,000 g 2~3,000 2-3,000 f/e 1/1 ～20/1 1/1 ～20/1 The size of the suitable cell culture test is described in Table 1, and it is suitable for culture. The size of the plant manufacturing and tissue culture. According to the size range shown in Table 1, the size of the convex portion 13 and the cell culture vessel 1 is set to obtain a cell culture vessel suitable for culture, or a culture plant and a container suitable for tissue culture. Here, f/e is 1/1 to 20/1. That is, the height of the first convex portion 11 is 1 to 20 times the width. Further, the size and width of the second convex portion 12 in the depth direction and the height of the -21 - 200804588 are set in the same range as the distance between the adjacent second convex portions 1 2 and d to g. Further, the first convex portion 丨丨 is set to be smaller than the second convex portion 12. In other words, the size and width of the second convex portion 1 2 in the depth direction can be set larger than the size and width of the first convex portion 1 1 in the depth direction. [Example 2] The shape of the cell culture vessel according to Example 2 will be described with reference to Fig. 7. Fig. 7 is a perspective view showing the shape of the cell culture container of the present embodiment. In Fig. 7, the second-order stepped convex portions 13 are arranged in a matrix. In other words, the cell culture container 10 of the present embodiment has a configuration in which the second convex portion 12 smaller than the first convex portion 11 is disposed on the upper surface of the first convex portion 1 1 . Thereby, the convex portions 13 composed of the first convex portion 1 1 and the second convex portion 1 2 are arranged in a matrix. The adjacent concave portions 14 communicate with each other on the upper side of the first convex portion 1 1 . The cells are cultured in the concave portion 14 between the convex portion 13 and the convex portion 13. That is, the concave portion 14 becomes a medium for the cells. A leap medium can be obtained by the shape as shown in Fig. 7. As shown in Fig. 7, the size of the cell culture vessel 1 in the depth direction is A, the width of the cell culture vessel is B, and the thickness (height) of the cell culture vessel 1 is C, and the depth of the first convex portion 11 is deep. The size is d 'the width of the first convex portion 为 is e, and the interval between the first convex portions 1 1 having the height of the first convex portion 1 1 being f ' is g. Here, the appropriate enthalpy of A, B, C, d, e, f, and g of the cell culture vessel 10 of the present embodiment is shown in Table 2. •11- 200804588 [Table 2] Dimensions < {β m) Culture test__ Culture plant manufacturing, tissue culture A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100-10,000 100-20,000 d 1-150,000 1-2,000,000 e 1-1,500 1- 2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1-20/1 1/1 ～20/1 The size of the cell culture test is described in Table 2, and it is suitable for the production of culture plants. The size of the tissue culture. A cell culture vessel suitable for culture, or a culture plant and a container suitable for tissue culture can be obtained by setting the size of the convex portion 13 and the cell culture vessel ’ according to the size range of the table 2 table. Here, f/e is 1/1 to 20/!. That is, the degree of the first convex portion 11 is 1 to 20 times the width. Further, the size, width, and height of the application direction of the second convex portion 1 2 and the interval between the adjacent second convex portions 12 are set to the same range as d to g. Further, the first convex portion 1 1 is set to be smaller than the second convex portion 12 . In other words, the size and width of the second convex portion 1 2 in the depth direction can be set larger than the size and width of the first convex portion 1 1 in the depth direction. [Example 3] The shape of the cell culture vessel according to Example 3 will be described with reference to Fig. 8. Fig. 8 is a perspective view showing the shape of the cell culture container of the present embodiment. In the present embodiment, a bioreactor or the like for the purpose of replacing the artificial internal organs can be used to explain the model of the culture liquid circulation. In Fig. 8, the first convex portion 1 1 and the second convex portion 1 2 which are higher than the first convex portion 1 1 are respectively disposed at positions not equal to -23-200804588. In other words, the first convex portion n and the second convex portion 12 having different heights are provided separately. Specifically, the second convex portions 1 2 are respectively provided at both ends of the cell culture container 10, and a plurality of first convex portions 丨丨 are provided therebetween. The second convex portion 1 2 is provided along the edge of the cell culture vessel 1 . The second convex portion 12 provided at both ends of the cell culture container 10 is higher than the first convex portion 11 provided at the center. In other words, the second convex portion 1 2 is a bridge higher than the side wall 16 of the first convex portion 1 1 . According to this, the pH can be maintained and the diffusion of substances from cells can be prevented. The first convex portions 1 1 are arranged in a line along the direction in which the second convex portions 1 2 are disposed. Further, each of the first convex portions 11 is provided in a direction orthogonal to the direction in which the second convex portions 12 are provided. The convex portions 13 are respectively extended in the direction. The concave portion 14 between the adjacent convex portions 1 3 serves as a concave groove through which the culture liquid flows. According to this, the culture solution flows in a direction orthogonal to the second convex portion 112. That is, the culture medium flowing through the center of the cell culture vessel 1 is flowed from one end to the other end. According to this, the diffusion of the generated substance can be prevented. Further, the adjacent concave portions 14 communicate with each other on the upper side of the first convex portion 1 1 . According to this, the culture solution can be circulated. As shown in Fig. 8, the size of the cell culture vessel 1 in the depth direction is A, the width of the cell culture vessel 10 is B, and the thickness (height) of the cell culture vessel 10 is C, and the depth of the first convex portion 1 is deep. The size of the direction is d 'the width of the first convex portion 1 1 is e, the height of the first convex portion 11 is f, and the interval between the adjacent first convex portions 11 is g. Here, the appropriate enthalpy of A, B, C, d, e, f, and g of the cell culture vessel 1 of the present embodiment is shown in Table 3. -24- 200804588 [Table 3] Dimensions < (μ m) Culture test culture Plant production, tissue culture A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000-2,000,000 C 100 ~ 100,000 100-200,000 d 1 〜150,000 1 〜2,000,000 e 1-1,500 1-2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1-20/1 1/1 ～20/1 The size of the suitable cell culture test is described in Table 3, and it is suitable for culture, tissue culture. size of. According to the size range shown in Table 3, the size of the convex portion 13 and the cell culture container 10 can be set to obtain a cell culture vessel suitable for culture, or a culture plant and a container suitable for tissue culture. Here, f / e is 1/1 to 20/1. That is, the degree of the first convex portion 11 is 1 to 20 times the width. Further, the size, width, and height of the second convex portion 12 in the depth direction and the interval between the adjacent second convex portions 12 are set to the same range as d to g. [Example 4] The shape of the cell culture vessel according to Example 4 will be described with reference to Fig. 9. Fig. 9 is a perspective view showing the shape of the cell culture container of the present embodiment. In the present embodiment, a bioreactor or the like for the purpose of replacing the artificial internal organs can be used to explain the model of the culture liquid circulation. The configuration of the cell culture vessel 10 according to the second embodiment will be described in the present embodiment. As shown in Fig. 9, in the cell culture container 10, a plurality of grooves 17 are formed by the bottom surface of the concave portion 14 formed by the first convex portion 11. The first convex portion 1 1 ' is provided at both ends of the cell culture vessel 10, that is, -25-200804588, and the two first convex portions 11 are disposed along the end sides of the cell culture vessel 10, respectively. Further, a plurality of grooves 17 are arranged in a matrix between the adjacent first convex portions 11. The first convex portion 1 1 forms a bridge higher than the side wall of the groove 17. As shown in Fig. 9, the size of the cell culture vessel 10 in the depth direction is A, the width of the cell culture vessel 10 is B, and the thickness (height) of the cell culture vessel 10 is C, and the depth of the groove 17 is deep. The size of the groove is d, the width of the groove 17 is e, the height of the groove 17 is f, and the interval between the adjacent grooves 17 is g. Here, the appropriate enthalpy of A, B, C, d, e, f and g of the cell culture vessel 10 of the present embodiment is shown in Table 4. [Table 4] Size < { U m) Culture of test cultures, tissue culture A 3,000-150,000 150,000-2,000,000 B 3,000-150,000 150,000~2,000,000 C 100 ~100,000 100-200,000 d 1-150,000 1-2,000,000 e 1-1,500 1-2,000 f 1-1,500 1-2,000 g 2-3,000 2-3,000 f/e 1/1 ～20/1 1/1 ～20/1 The size of the cell culture test is described in Table 4, and it is suitable for culture, tissue culture. size of. According to the size range shown in Table 4, the size of the convex portion 13 and the cell culture vessel 丨〇 can be set, and a cell culture vessel </ RTI> suitable for culture or a culture plant can be obtained and a container suitable for tissue culture can be obtained. Here, ideally f / e is 1 / 1 ~ 2 0 / 1. That is, the depth of the groove 17 is 1 to 20 times the width. Further, the size, width, and width of the first convex portion u in the depth direction and the interval between the adjacent first convex portions 1 1 are set to be the same as those of d to g. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a plan view showing the constitution of a cell culture vessel according to an embodiment of the present invention. Fig. 2 is a cross-sectional view showing the constitution of a cell culture vessel according to Embodiment 1 of the present invention. Fig. 3 is a plan view showing the configuration of the cell culture container according to the second embodiment. Fig. 4 is an enlarged plan view showing the constitution of a cell culture container according to a second embodiment of the present invention. Fig. 5 is a cross-sectional view showing the constitution of a cell culture vessel according to a second embodiment of the present invention. Fig. 6 is a perspective view showing the constitution of a cell culture vessel according to an embodiment of the present invention. Fig. 7 is a perspective view showing the constitution of a cell culture container according to Example 2 of the present invention. Fig. 8 is a perspective view showing the constitution of a cell culture container according to Example 3 of the present invention. Fig. 9 is a perspective view showing the constitution of a cell culture container according to Example 4 of the present invention. [Main component symbol description] 10 Cell culture container 11 First convex portion 12 Second convex portion 13 convex portion -27- 200804588 14 Concave portion 15 Side wall of the first convex portion 16 Side wall of the second convex portion 17 Groove -28