TR201904022T4 - Konakçı hücre modifikasyon yöntemleri. - Google Patents

Konakçı hücre modifikasyon yöntemleri. Download PDF

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Publication number
TR201904022T4
TR201904022T4 TR2019/04022T TR201904022T TR201904022T4 TR 201904022 T4 TR201904022 T4 TR 201904022T4 TR 2019/04022 T TR2019/04022 T TR 2019/04022T TR 201904022 T TR201904022 T TR 201904022T TR 201904022 T4 TR201904022 T4 TR 201904022T4
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host cell
modification methods
cell modification
host cells
methods
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TR2019/04022T
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Wacker Michael
Kowarik Michael
Fernandez Fabiana
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Glaxosmithkline Biologicals Sa
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Abstract

Nükleik asit sekanslarının konakçı hücreler içine sokulmasına yönelik yeni yöntemler burada tarif edilir. Burada ayrıca sokulmuş nükleik asit sekanslarını içeren, genetik açıdan stabil konakçı hücreler ve bu konakçı hücrelerin, proteinlerin üretilmesinde kullanılmasına yönelik yöntemler tarif edilir.
TR2019/04022T 2012-10-12 2013-10-11 Konakçı hücre modifikasyon yöntemleri. TR201904022T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261713281P 2012-10-12 2012-10-12

Publications (1)

Publication Number Publication Date
TR201904022T4 true TR201904022T4 (tr) 2019-04-22

Family

ID=49326691

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Application Number Title Priority Date Filing Date
TR2019/04022T TR201904022T4 (tr) 2012-10-12 2013-10-11 Konakçı hücre modifikasyon yöntemleri.

Country Status (16)

Country Link
US (2) US20150267207A1 (tr)
EP (2) EP3527663B1 (tr)
JP (1) JP6329554B2 (tr)
KR (1) KR20150068998A (tr)
CN (2) CN104837984A (tr)
AU (1) AU2013328615B2 (tr)
BR (1) BR112015008052A2 (tr)
CA (1) CA2887133C (tr)
EA (1) EA201590488A1 (tr)
ES (2) ES2905107T3 (tr)
IL (1) IL238024A0 (tr)
MX (1) MX365435B (tr)
SG (2) SG11201502638YA (tr)
TR (1) TR201904022T4 (tr)
WO (1) WO2014057109A1 (tr)
ZA (1) ZA201502433B (tr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2917351B1 (en) 2012-11-07 2018-12-19 GlaxoSmithKline Biologicals S.A. Production of recombinant vaccine in e. coli by enzymatic conjugation
WO2014111516A1 (en) 2013-01-17 2014-07-24 Arsanis Biosciences Gmbh Mdr e. coli specific antibody
SG11201508347YA (en) * 2013-03-14 2015-11-27 Glycobia Inc Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
SG11201602546RA (en) * 2013-10-11 2016-04-28 Glycovaxyn Ag Methods of host cell modification
FI3110441T3 (fi) 2014-02-24 2024-05-06 Glaxosmithkline Biologicals Sa Uusi polysakkaridi ja sen käyttöjä
SI3131577T1 (sl) * 2014-04-17 2020-08-31 Glaxosmithkline Biologicals S.A. Modificirane gostiljske celice in uporabe le-teh
WO2016025759A1 (en) * 2014-08-14 2016-02-18 Shen Yuelei Dna knock-in system
US10265391B2 (en) 2015-02-26 2019-04-23 Vaxnewmo Llc Acinetobacter O-oligosaccharyltransferases and uses thereof
TWI715617B (zh) 2015-08-24 2021-01-11 比利時商葛蘭素史密斯克藍生物品公司 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物
GB201518668D0 (en) 2015-10-21 2015-12-02 Glaxosmithkline Biolog Sa Immunogenic Comosition
GB201610599D0 (en) 2016-06-17 2016-08-03 Glaxosmithkline Biologicals Sa Immunogenic Composition
AR109621A1 (es) 2016-10-24 2018-12-26 Janssen Pharmaceuticals Inc Formulaciones de vacunas contra glucoconjugados de expec
CN106801029B (zh) * 2017-01-22 2020-05-01 山东大学 一株重组大肠杆菌及其在制备抗志贺氏菌的糖疫苗中的应用
CN109385417A (zh) * 2017-08-03 2019-02-26 华东理工大学 体内dna无缝组装方法
GB201712678D0 (en) 2017-08-07 2017-09-20 Glaxosmithkline Biologicals Sa Process for the manipulation of nucleic acids
GB201721582D0 (en) 2017-12-21 2018-02-07 Glaxosmithkline Biologicals Sa S aureus antigens and immunogenic compositions
GB201721576D0 (en) 2017-12-21 2018-02-07 Glaxosmithkline Biologicals Sa Hla antigens and glycoconjugates thereof
US11932670B2 (en) 2018-06-16 2024-03-19 Vaxnewmo Llc Glycosylated ComP pilin variants, methods of making and uses thereof
US11260119B2 (en) 2018-08-24 2022-03-01 Pfizer Inc. Escherichia coli compositions and methods thereof
MX2021005028A (es) * 2018-10-31 2021-08-24 Zymergen Inc Ensamble determinista multiplexado de genotecas de adn.
MX2021011445A (es) 2019-03-18 2021-10-13 Janssen Pharmaceuticals Inc Bioconjugados de polisacaridos de antigenos o de e. coli, metodos de produccion y metodos de uso de los mismos.
JP2022525773A (ja) 2019-03-18 2022-05-19 ヤンセン ファーマシューティカルズ,インコーポレーテッド 大腸菌o抗原多糖のバイオコンジュゲートを製造する方法、その組成物およびその使用方法
EP3770269A1 (en) 2019-07-23 2021-01-27 GlaxoSmithKline Biologicals S.A. Quantification of bioconjugate glycosylation
WO2021255684A1 (en) * 2020-06-18 2021-12-23 Glaxosmithkline Biologicals Sa Shigella-tetravalent (shigella4v) bioconjugate
CA3185642A1 (en) 2020-06-25 2021-12-30 Glaxosmithkline Biologicals Sa Modified exotoxin a proteins
CN115803087A (zh) 2020-09-17 2023-03-14 杨森制药公司 多价疫苗组合物及其用途
JP2022099419A (ja) * 2020-12-23 2022-07-05 トヨタ自動車株式会社 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法
WO2022171679A2 (en) 2021-02-11 2022-08-18 Glaxosmithkline Biologicals Sa Protein
CN117098550A (zh) 2021-04-08 2023-11-21 杨森制药公司 生物缀合物生产方法
CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技(广州)有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用
WO2023118033A1 (en) 2021-12-22 2023-06-29 Glaxosmithkline Biologicals Sa Vaccine

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5057540A (en) 1987-05-29 1991-10-15 Cambridge Biotech Corporation Saponin adjuvant
US4912094B1 (en) 1988-06-29 1994-02-15 Ribi Immunochem Research Inc. Modified lipopolysaccharides and process of preparation
CA2184688A1 (en) * 1994-03-09 1995-09-14 Pedro Antonio Prieto Transgenic animals producing oligosaccharides and glycoconjugates
US6596541B2 (en) * 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
WO2002038755A1 (en) * 2000-11-07 2002-05-16 Jefferies Wilfred A Circular extra-chromosomal dna elements
US7126039B2 (en) * 2001-03-21 2006-10-24 Geron Corporation Animal tissue with carbohydrate antigens compatible for human transplantation
US7674621B2 (en) * 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
WO2006130925A1 (en) * 2005-06-10 2006-12-14 Monash University Genetic manipulation of clostridium difficile
WO2007109813A1 (en) 2006-03-23 2007-09-27 Novartis Ag Imidazoquinoxaline compounds as immunomodulators
CA2646891A1 (en) 2006-03-23 2007-09-27 Novartis Ag Immunopotentiating compounds
EP3427749A1 (en) * 2008-02-20 2019-01-16 GlaxoSmithKline Biologicals SA Bioconjugates made from recombinant n-glycosylated proteins from procaryotic cells
EP3281639B1 (en) 2010-05-06 2020-11-11 GlaxoSmithKline Biologicals S.A. Capsular gram-positive bacteria bioconjugate vaccines

Also Published As

Publication number Publication date
ES2720040T3 (es) 2019-07-17
AU2013328615A1 (en) 2015-04-30
CN110423716A (zh) 2019-11-08
SG10201800777PA (en) 2018-02-27
SG11201502638YA (en) 2015-05-28
US20150267207A1 (en) 2015-09-24
KR20150068998A (ko) 2015-06-22
ZA201502433B (en) 2017-11-29
US20190218561A1 (en) 2019-07-18
EA201590488A1 (ru) 2015-09-30
MX2015004649A (es) 2016-01-12
CA2887133C (en) 2022-05-03
JP2015533497A (ja) 2015-11-26
MX365435B (es) 2019-06-03
ES2905107T3 (es) 2022-04-07
EP3527663B1 (en) 2021-12-01
EP2906681A1 (en) 2015-08-19
EP2906681B1 (en) 2019-01-09
EP3527663A1 (en) 2019-08-21
CN104837984A (zh) 2015-08-12
CA2887133A1 (en) 2014-04-17
IL238024A0 (en) 2015-05-31
JP6329554B2 (ja) 2018-05-23
WO2014057109A1 (en) 2014-04-17
AU2013328615B2 (en) 2017-04-20
BR112015008052A2 (pt) 2017-11-28

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