SU24543A1 - Method and apparatus for growing bacteria - Google Patents
Method and apparatus for growing bacteriaInfo
- Publication number
- SU24543A1 SU24543A1 SU79179A SU79179A SU24543A1 SU 24543 A1 SU24543 A1 SU 24543A1 SU 79179 A SU79179 A SU 79179A SU 79179 A SU79179 A SU 79179A SU 24543 A1 SU24543 A1 SU 24543A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- bacteria
- nutrient medium
- vessel
- growing
- liquid nutrient
- Prior art date
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Способ выращивани бактерий и прибор дл осуществлени означенного способа заключаетс в комбинации пористой мембраны и жидкого питательного бульона. В качестве мембраны примен ют различные пористые пленки, величину пор которых можно регулировать, по желанию, различными методами, как напр, применением уксуснокислых или спиртоэфирных растворов коллоди различных концентраций, различным соотношением спирта и эфира в .растворе, добавлением глицерина, погружением коллодийной пленки в воду через различные промежутки времени после ее приготовлени и т. д.The method of growing bacteria and the apparatus for carrying out the aforementioned method consists in the combination of a porous membrane and a liquid nutrient broth. Various porous films are used as membranes, the pore size of which can be adjusted, as desired, by various methods, such as by using acetic acid or alcohol ether collodi solutions of various concentrations, different ratios of alcohol and ether in solution, adding glycerol, immersing the collodion film in water through various periods of time after its preparation, etc.
Мембрана с монтируетс в приборе дл выращивани бактерий, изображенном на схематическом чертеже (фиг. 1) в виде плоского сосуда таким образом, что, будучи зажата между двум резиновыми прокладками е, е, наход щимис на внутренних в|,1стуиах стенок сосуда, она закрывает собою полость, наполненную жидкой питательной средой. Стекл ннре дно d сосуда зажато при помощи бинта и резиновых прокладок в металлическую оправу f.The membrane with is mounted in the bacterium growing device shown in the schematic drawing (Fig. 1) as a flat vessel in such a way that, being sandwiched between two rubber pads e, e, located on the inner walls of the vessel, it closes itself a cavity filled with liquid nutrient medium. The glass bottom of the vessel is clamped with a bandage and rubber gaskets in a metal frame f.
Полость сосуда снабжена двум трубками (фиг. 2), из которых одна служит дл наливани жидкой питательной средыThe cavity of the vessel is equipped with two tubes (Fig. 2), one of which serves to pour the liquid nutrient medium
в нижнюю часть сосуда, друга же дл выпуска и впуска воздуха при наполнении и опоражнивании его. Дл защиты, от загр знений сосуд накрываетс сверху стекл нной крышкой или колпаком с зажимаемым при помощи добавочного кольца с боковым тубусом /, дл засева бактерий и сн ти роста их.in the lower part of the vessel, the other for the release and admission of air during filling and emptying it. For protection, the vessel is covered from above with a glass lid or a cap with a clamped with an additional ring with a side tube /, for seeding bacteria and removing their growth.
Засев бактерий производитс обычным образом на поверхности мембраны, омываемой с другой стороны жидкой питательной средой. Описанный метод вл етс заменой примен емых до сих пор твердых питательных сред дл бактерий, получающихс с помощью прибавлени к жидкой питательной среде агар-агара дл получени студнеобразно - застывающей при охлаждении массы.Bacteria are sown in the usual manner on the surface of a membrane washed on the other side with a liquid nutrient medium. The described method is a substitute for the solid nutrient media used so far for bacteria, obtained by adding agar-agar to the liquid nutrient medium to obtain jelly-like - solidifying during cooling mass.
Предмет изобретени .The subject matter of the invention.
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU79179A SU24543A1 (en) | 1930-11-20 | 1930-11-20 | Method and apparatus for growing bacteria |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU79179A SU24543A1 (en) | 1930-11-20 | 1930-11-20 | Method and apparatus for growing bacteria |
Publications (2)
Publication Number | Publication Date |
---|---|
SU1072A1 SU1072A1 (en) | 1926-02-27 |
SU24543A1 true SU24543A1 (en) | 1931-12-31 |
Family
ID=48342842
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU79179A SU24543A1 (en) | 1930-11-20 | 1930-11-20 | Method and apparatus for growing bacteria |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU24543A1 (en) |
-
1930
- 1930-11-20 SU SU79179A patent/SU24543A1/en active
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