SG11201903557SA - Methods for screening b cell lymphocytes - Google Patents

Methods for screening b cell lymphocytes

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Publication number
SG11201903557SA
SG11201903557SA SG11201903557SA SG11201903557SA SG11201903557SA SG 11201903557S A SG11201903557S A SG 11201903557SA SG 11201903557S A SG11201903557S A SG 11201903557SA SG 11201903557S A SG11201903557S A SG 11201903557SA SG 11201903557S A SG11201903557S A SG 11201903557SA
Authority
SG
Singapore
Prior art keywords
emeryville
suite
street
international
horton
Prior art date
Application number
SG11201903557SA
Inventor
Minha Park
Jason Briggs
Jason Mcewen
Ravi Ramenani
Dinakar Hariharasudhan Chirra
Kai Szeto
Adrienne Higa
Mark White
Randall Lowe
Xiaohua Wang
Kevin Chapman
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201903557SA publication Critical patent/SG11201903557SA/en

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    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111011111111101 IIIIIIIII101111101011H011111110011111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/076024 A2 26 April 2018 (26.04.2018) WIP0 I PCT (51) International Patent Classification: NANI, Ravinder, K. [IN/US]; 5858 Horton Street, Suite GO1N 33/574 (2006.01) C12Q 1/68 (2006.01) 320, Emeryville, CA 94608 (US). CHIRRA DINAKAR, (21) International Application Number: Hariharasudhan [IN/US]; 5858 Horton Street, Suite 320, PCT/US2017/057926 Emeryville, CA 94608 (US). SZETO, Kai, W. [US/US]; 5858 Horton Street, Suite 320, Emeryville, CA 94608 (22) International Filing Date: (US). HIGA, Adrienne, T. [US/US]; 5858 Horton Street, 23 October 2017 (23.10.2017) Suite 320, Emeryville, CA 94608 (US). WHITE, Mark, P. [US/US]; 5858 Horton Street, Suite 320, Emeryville, (25) Filing Language: English CA 94608 (US). LOWE, Randall, D. [US/US]; 5858 (26) Publication Language: English Horton Street, Suite 320, Emeryville, CA 94608 (US). (30) Priority Data: WANG, Xiaohua [CN/US]; 8203 Overhill Dr., Pomona, NY 10970 (US). CHAPMAN, Kevin, T. [US/US]; 5858 62/411,690 23 October 2016 (23.10.2016) US Horton Street Suite 320, Emeryville, CA 94608 (US). 62/412,092 24 October 2016 (24.10.2016) US (81) Designated States (unless otherwise indicated, for every (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 kind of national protection available): AE, AG, AL, AM, Horton Street, Suite 320, Emeryville, CA 94608 (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (72) Inventors; and CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (71) Applicants: PARK, Minha [US/US]; 5858 Horton Street, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, Suite 320, Emeryville, CA 94608 (US). BRIGGS, Jason, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, _ C. [US/US]; 5858 Horton Street, Suite 320, Emeryville, CA KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 94608 (US). MCEWEN, Jason, M. [US/US]; 5858 Hor- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = ton Street, Suite 320, Emeryville, CA 94608 (US). RAME- OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, = (54) Title: METHODS FOR SCREENING B CELL LYMPHOCYTES — = = = = _ _ = = _ ,1 , ,, , t• = _ ,,, = = = \ = _ = ‘ ei ii- ei FIG. 6D © IN c: : :: , (57) : Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody -- --- GO producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be 0 monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described. ei O [Continued on next page] WO 2018/076024 A2IMIOHOIMIREIH00 IIIIIMEIRIMME110IS SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g))
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