SG11201809685XA - A method of enhancing the performance of chromatography methods for purification of proteins - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 December 2017 (21.12.2017) WIP0 I PCT iii 1111111111111111111111111111111111111111111111111111111111111111101111111111111111111 (10) International Publication Number WO 2017/217930 Al (51) International Patent Classification: C07K 1/30 (2006.01) (21) International Application Number: PCT/SG2017/050294 (22) International Filing Date: 12 June 2017 (12.06.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201604895U 15 June 2016 (15.06.2016) SG 10201604898V 15 June 2016 (15.06.2016) SG 10201702562T 29 March 2017 (29.03.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: GAGNON, Peter Stanley; c/o Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, Singapore 138668 (SG). TOH, Yan Ling Phyllicia; c/o Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, Singapore 138668 (SG). ZHANG, Wei; c/o Bioprocessing Technolo- gy Institute, 20 Biopolis Way, #06-01, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) 1-1 O t:T N 1-1 N _ N O 1-1 N C (54) Title: A METHOD OF ENHANCING THE PERFORMANCE OF CHROMATOGRAPHY METHODS FOR PURIFICATION OF PROTEINS (57) : This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. The method is exemplified by a combination of allantoin, caprylic acid and a cationic polymer selected from chitosan, polydiallyldimethylammonium chloride (pDADMAC) and polyallylamine. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.