SG11201407809QA - Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities - Google Patents
Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalitiesInfo
- Publication number
- SG11201407809QA SG11201407809QA SG11201407809QA SG11201407809QA SG11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- chromatographic column
- packed
- sample
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/54—F(ab')2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180647 A1 (51) International Patent Classification: C07K1/16 (2006.01) C07K16/00 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000031 21 January 2013 (21.01.2013) English English (30) Priority Data: 61/653,913 31 May 2012 (31.05.2012) US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionpolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter, Stanley; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; 1531 Robinson Road Post Office, Singapore 903031 (SG). BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available)-. AE, AG, AL, AM, _ . ^ international search t (Art 21(3) ) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, F 1 1 r- •t o 00 i-H en i-H o CJ o & (54) Title: CHROMATOGRAPHIC PURIFICATION OF IMMUNOGLOBULIN G PREPARATIONS WITH PARTICLES HAV ING MULTIMODAL FUNCTIONALITIES (57) Abstract: A method of purifying sample a containing a desired protein includes the steps of (i) providing a packed chromato graphic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired pro tein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume ap - plied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the de sired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the de - sired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein. (No suitable Figure)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261653913P | 2012-05-31 | 2012-05-31 | |
PCT/SG2013/000031 WO2013180647A1 (en) | 2012-05-31 | 2013-01-21 | Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201407809QA true SG11201407809QA (en) | 2014-12-30 |
Family
ID=49673718
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201407809QA SG11201407809QA (en) | 2012-05-31 | 2013-01-21 | Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities |
Country Status (8)
Country | Link |
---|---|
US (1) | US9890205B2 (en) |
EP (1) | EP2855504B1 (en) |
JP (1) | JP6297029B2 (en) |
KR (1) | KR20150027060A (en) |
CN (1) | CN104507955A (en) |
IN (1) | IN2014DN09962A (en) |
SG (1) | SG11201407809QA (en) |
WO (1) | WO2013180647A1 (en) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102067445B1 (en) * | 2012-05-31 | 2020-01-17 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | Methods for reducing levels of protein-contaminant complexes and aggregates in protein preparations by treatment with electropositive organic additives |
CN105121457B (en) * | 2013-02-22 | 2019-04-30 | 新加坡科技研究局 | For removing endotoxic material and method from protein formulation |
KR20150122650A (en) * | 2013-02-28 | 2015-11-02 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | Protein purification in the presence of nonionic organic polymers at elevated conductivity |
KR20160122754A (en) * | 2014-02-19 | 2016-10-24 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | Fractionation method |
CN106164086A (en) * | 2014-02-27 | 2016-11-23 | 新加坡科技研究局 | Antibody purification process |
KR20160127087A (en) * | 2014-02-27 | 2016-11-02 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | Methods for reducing chromatin content in protein preparations by treatment with alkyl cations |
CN106103465B (en) * | 2014-03-10 | 2021-01-08 | 吉瑞工厂 | Immunoglobulin purification using a precleaning step |
JP6599980B2 (en) * | 2014-05-29 | 2019-10-30 | エイジェンシー・フォー・サイエンス,テクノロジー・アンド・リサーチ | Protein extraction method |
CN104356211B (en) * | 2014-09-22 | 2017-08-29 | 山东信得科技股份有限公司 | One kind application Triton X 114 and allantoin remove endotoxic method in recombinant protein solution |
WO2016156153A1 (en) | 2015-03-31 | 2016-10-06 | Ge Healthcare Bio-Sciences Ab | A liquid chromatograhy system, a device, and a method |
EP3371302B1 (en) * | 2015-11-05 | 2024-01-03 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for the separation of virus compositions including depletion and purification thereof |
US20190039046A1 (en) * | 2016-02-25 | 2019-02-07 | Hitachi, Ltd. | Adsorbent material and purification method |
WO2018092691A1 (en) * | 2016-11-18 | 2018-05-24 | Jnc株式会社 | Method for purifying antibody |
EP3581578A4 (en) * | 2017-02-10 | 2020-04-22 | Mitsubishi Chemical Corporation | Separating agent for human insulin purification and human insulin purification method |
KR20200138346A (en) * | 2018-04-03 | 2020-12-09 | 메르크 파텐트 게엠베하 | Low salt elution of target proteins from CEX chromatography media and biopharmaceutical feeds |
US20220315622A1 (en) | 2019-09-06 | 2022-10-06 | Bia Separations D.O.O. | Compositions and methods for reducing chromatin content of biological preparations |
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JPH0666793A (en) * | 1984-09-14 | 1994-03-11 | Medekusu:Kk | Antibody specific to protein only appearing from rheumatism |
US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
US5691132A (en) | 1994-11-14 | 1997-11-25 | Cerus Corporation | Method for inactivating pathogens in red cell compositions using quinacrine mustard |
ATE277950T1 (en) * | 1998-06-09 | 2004-10-15 | Statens Seruminstitut | METHOD FOR PRODUCING IMMUNOGLOBULINS FOR INTRAVENOUS ADMINISTRATION AND OTHER IMMUNOGLOBULIN PRODUCTS |
DK1200179T3 (en) | 1999-07-30 | 2006-09-25 | Genentech Inc | Charged filtration membranes and applications thereof |
GB0216002D0 (en) * | 2002-07-10 | 2002-08-21 | Nat Blood Authority | Process and composition |
CA2548220A1 (en) * | 2003-12-01 | 2005-09-09 | Introgen Therapeutics, Inc. | Use of mda-7 to inhibit infection by pathogenic organisms |
US20070244305A1 (en) * | 2004-01-30 | 2007-10-18 | Suomen Punainen Risti Veripalvelu | Process for The Manufacture of Virus Safe Immunoglobulin |
ES2407380T5 (en) * | 2004-02-27 | 2017-07-07 | Octapharma Ag | Procedure to provide a purified antibody preparation, with no risk for viruses |
NZ554520A (en) * | 2004-10-22 | 2010-03-26 | Amgen Inc | Methods for refolding of recombinant antibodies |
PL1841863T3 (en) * | 2005-01-14 | 2011-05-31 | Bayer Healthcare Llc | Method for purification of factor vii |
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AU2011317021B2 (en) * | 2010-10-20 | 2015-07-09 | Medimmune, Llc | Methods for processing inclusion bodies |
CN102286099B (en) * | 2011-08-05 | 2014-04-09 | 深圳市卫光生物制品股份有限公司 | Human cytomegalovirus immunoglobulin for intravenous injection and preparation method thereof |
CN105121457B (en) * | 2013-02-22 | 2019-04-30 | 新加坡科技研究局 | For removing endotoxic material and method from protein formulation |
JP2016509068A (en) * | 2013-02-28 | 2016-03-24 | エイジェンシー・フォー・サイエンス,テクノロジー・アンド・リサーチ | Chromatographic purification of antibodies from chromatin-deficient cell culture harvests. |
US10174076B2 (en) * | 2013-06-04 | 2019-01-08 | Agency For Science, Technology And Research | Protein purification process |
-
2013
- 2013-01-21 EP EP13797544.7A patent/EP2855504B1/en not_active Not-in-force
- 2013-01-21 JP JP2015514962A patent/JP6297029B2/en not_active Expired - Fee Related
- 2013-01-21 WO PCT/SG2013/000031 patent/WO2013180647A1/en active Application Filing
- 2013-01-21 KR KR1020147033090A patent/KR20150027060A/en active IP Right Grant
- 2013-01-21 SG SG11201407809QA patent/SG11201407809QA/en unknown
- 2013-01-21 CN CN201380040418.9A patent/CN104507955A/en active Pending
-
2014
- 2014-11-25 IN IN9962DEN2014 patent/IN2014DN09962A/en unknown
- 2014-11-26 US US14/555,060 patent/US9890205B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP2855504B1 (en) | 2018-10-24 |
EP2855504A1 (en) | 2015-04-08 |
JP6297029B2 (en) | 2018-03-20 |
EP2855504A4 (en) | 2015-07-29 |
US9890205B2 (en) | 2018-02-13 |
JP2015519360A (en) | 2015-07-09 |
US20150183852A1 (en) | 2015-07-02 |
CN104507955A (en) | 2015-04-08 |
KR20150027060A (en) | 2015-03-11 |
WO2013180647A1 (en) | 2013-12-05 |
IN2014DN09962A (en) | 2015-08-14 |
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