SG11201407809QA - Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities - Google Patents

Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Info

Publication number
SG11201407809QA
SG11201407809QA SG11201407809QA SG11201407809QA SG11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA
Authority
SG
Singapore
Prior art keywords
international
chromatographic column
packed
sample
protein
Prior art date
Application number
SG11201407809QA
Inventor
Peter Stanley Gagnon
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201407809QA publication Critical patent/SG11201407809QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180647 A1 (51) International Patent Classification: C07K1/16 (2006.01) C07K16/00 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000031 21 January 2013 (21.01.2013) English English (30) Priority Data: 61/653,913 31 May 2012 (31.05.2012) US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionpolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter, Stanley; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; 1531 Robinson Road Post Office, Singapore 903031 (SG). BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available)-. AE, AG, AL, AM, _ . ^ international search t (Art 21(3) ) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, F 1 1 r- •t o 00 i-H en i-H o CJ o & (54) Title: CHROMATOGRAPHIC PURIFICATION OF IMMUNOGLOBULIN G PREPARATIONS WITH PARTICLES HAV­ ING MULTIMODAL FUNCTIONALITIES (57) Abstract: A method of purifying sample a containing a desired protein includes the steps of (i) providing a packed chromato­ graphic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired pro­ tein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume ap - plied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the de­ sired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the de - sired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein. (No suitable Figure)
SG11201407809QA 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities SG11201407809QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653913P 2012-05-31 2012-05-31
PCT/SG2013/000031 WO2013180647A1 (en) 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Publications (1)

Publication Number Publication Date
SG11201407809QA true SG11201407809QA (en) 2014-12-30

Family

ID=49673718

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201407809QA SG11201407809QA (en) 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Country Status (8)

Country Link
US (1) US9890205B2 (en)
EP (1) EP2855504B1 (en)
JP (1) JP6297029B2 (en)
KR (1) KR20150027060A (en)
CN (1) CN104507955A (en)
IN (1) IN2014DN09962A (en)
SG (1) SG11201407809QA (en)
WO (1) WO2013180647A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102067445B1 (en) * 2012-05-31 2020-01-17 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Methods for reducing levels of protein-contaminant complexes and aggregates in protein preparations by treatment with electropositive organic additives
CN105121457B (en) * 2013-02-22 2019-04-30 新加坡科技研究局 For removing endotoxic material and method from protein formulation
KR20150122650A (en) * 2013-02-28 2015-11-02 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Protein purification in the presence of nonionic organic polymers at elevated conductivity
KR20160122754A (en) * 2014-02-19 2016-10-24 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Fractionation method
CN106164086A (en) * 2014-02-27 2016-11-23 新加坡科技研究局 Antibody purification process
KR20160127087A (en) * 2014-02-27 2016-11-02 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Methods for reducing chromatin content in protein preparations by treatment with alkyl cations
CN106103465B (en) * 2014-03-10 2021-01-08 吉瑞工厂 Immunoglobulin purification using a precleaning step
JP6599980B2 (en) * 2014-05-29 2019-10-30 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ Protein extraction method
CN104356211B (en) * 2014-09-22 2017-08-29 山东信得科技股份有限公司 One kind application Triton X 114 and allantoin remove endotoxic method in recombinant protein solution
WO2016156153A1 (en) 2015-03-31 2016-10-06 Ge Healthcare Bio-Sciences Ab A liquid chromatograhy system, a device, and a method
EP3371302B1 (en) * 2015-11-05 2024-01-03 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for the separation of virus compositions including depletion and purification thereof
US20190039046A1 (en) * 2016-02-25 2019-02-07 Hitachi, Ltd. Adsorbent material and purification method
WO2018092691A1 (en) * 2016-11-18 2018-05-24 Jnc株式会社 Method for purifying antibody
EP3581578A4 (en) * 2017-02-10 2020-04-22 Mitsubishi Chemical Corporation Separating agent for human insulin purification and human insulin purification method
KR20200138346A (en) * 2018-04-03 2020-12-09 메르크 파텐트 게엠베하 Low salt elution of target proteins from CEX chromatography media and biopharmaceutical feeds
US20220315622A1 (en) 2019-09-06 2022-10-06 Bia Separations D.O.O. Compositions and methods for reducing chromatin content of biological preparations

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0666793A (en) * 1984-09-14 1994-03-11 Medekusu:Kk Antibody specific to protein only appearing from rheumatism
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US5691132A (en) 1994-11-14 1997-11-25 Cerus Corporation Method for inactivating pathogens in red cell compositions using quinacrine mustard
ATE277950T1 (en) * 1998-06-09 2004-10-15 Statens Seruminstitut METHOD FOR PRODUCING IMMUNOGLOBULINS FOR INTRAVENOUS ADMINISTRATION AND OTHER IMMUNOGLOBULIN PRODUCTS
DK1200179T3 (en) 1999-07-30 2006-09-25 Genentech Inc Charged filtration membranes and applications thereof
GB0216002D0 (en) * 2002-07-10 2002-08-21 Nat Blood Authority Process and composition
CA2548220A1 (en) * 2003-12-01 2005-09-09 Introgen Therapeutics, Inc. Use of mda-7 to inhibit infection by pathogenic organisms
US20070244305A1 (en) * 2004-01-30 2007-10-18 Suomen Punainen Risti Veripalvelu Process for The Manufacture of Virus Safe Immunoglobulin
ES2407380T5 (en) * 2004-02-27 2017-07-07 Octapharma Ag Procedure to provide a purified antibody preparation, with no risk for viruses
NZ554520A (en) * 2004-10-22 2010-03-26 Amgen Inc Methods for refolding of recombinant antibodies
PL1841863T3 (en) * 2005-01-14 2011-05-31 Bayer Healthcare Llc Method for purification of factor vii
US8067182B2 (en) * 2005-03-11 2011-11-29 Wyeth Llc Method of weak partitioning chromatography
KR20140071452A (en) 2006-04-05 2014-06-11 애브비 바이오테크놀로지 리미티드 Antibody purification
WO2007136327A1 (en) * 2006-05-22 2007-11-29 Ge Healthcare Bio-Sciences Ab A method of producing igg
US20100234577A1 (en) * 2006-06-14 2010-09-16 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
ATE526017T1 (en) * 2007-01-22 2011-10-15 Genentech Inc DEPOSITION OF POLYELECTROLYTES AND PURIFICATION OF ANTIBODIES
CN101306200B (en) * 2008-07-04 2011-04-06 四川远大蜀阳药业股份有限公司 Preparation method of hepatitis b human immunoglobulin for intravenous injection
MX2011001696A (en) * 2008-08-14 2011-03-25 Merck Sharp & Dohme Methods for purifying antibodies using protein a affinity chromatography.
AU2009307728B2 (en) * 2008-10-20 2014-12-11 Abbvie Inc. Antibodies that bind to IL-18 and methods of purifying the same
US20120282654A1 (en) * 2009-04-29 2012-11-08 Schering Corporation Antibody purification
IT1397061B1 (en) * 2009-12-28 2012-12-28 Kedrion Spa NEW PROCESS OF PURIFICATION ON INDUSTRIAL SCALE OF GAMMAGLOBULINE FROM HUMAN PLASMA FOR INDUSTRIAL USE.
US20130023436A1 (en) * 2010-01-15 2013-01-24 Bryan William Jones Disease diagnosis and treatment using computational molecular phenotyping
GB201012599D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
PL3434346T3 (en) * 2010-07-30 2021-04-06 Medimmune, Llc Purified active polypeptides or immunoconjugates
AU2011317021B2 (en) * 2010-10-20 2015-07-09 Medimmune, Llc Methods for processing inclusion bodies
CN102286099B (en) * 2011-08-05 2014-04-09 深圳市卫光生物制品股份有限公司 Human cytomegalovirus immunoglobulin for intravenous injection and preparation method thereof
CN105121457B (en) * 2013-02-22 2019-04-30 新加坡科技研究局 For removing endotoxic material and method from protein formulation
JP2016509068A (en) * 2013-02-28 2016-03-24 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ Chromatographic purification of antibodies from chromatin-deficient cell culture harvests.
US10174076B2 (en) * 2013-06-04 2019-01-08 Agency For Science, Technology And Research Protein purification process

Also Published As

Publication number Publication date
EP2855504B1 (en) 2018-10-24
EP2855504A1 (en) 2015-04-08
JP6297029B2 (en) 2018-03-20
EP2855504A4 (en) 2015-07-29
US9890205B2 (en) 2018-02-13
JP2015519360A (en) 2015-07-09
US20150183852A1 (en) 2015-07-02
CN104507955A (en) 2015-04-08
KR20150027060A (en) 2015-03-11
WO2013180647A1 (en) 2013-12-05
IN2014DN09962A (en) 2015-08-14

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