SG11201804706UA - Open-top microfluidic devices and methods for simulating a function of a tissue - Google Patents

Open-top microfluidic devices and methods for simulating a function of a tissue

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Publication number
SG11201804706UA
SG11201804706UA SG11201804706UA SG11201804706UA SG11201804706UA SG 11201804706U A SG11201804706U A SG 11201804706UA SG 11201804706U A SG11201804706U A SG 11201804706UA SG 11201804706U A SG11201804706U A SG 11201804706UA SG 11201804706U A SG11201804706U A SG 11201804706UA
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SG
Singapore
Prior art keywords
massachusetts
chamber
international
street
membrane
Prior art date
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SG11201804706UA
Inventor
Antonio Varone
Norman Wen
Daniel Levner
Richard Novak
Lori Mcpartlin
Donald Ingber
Youngjae Choe
Lian Leng
Justin Nguyen
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Harvard College
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Application filed by Harvard College filed Critical Harvard College
Publication of SG11201804706UA publication Critical patent/SG11201804706UA/en

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    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
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    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/04—Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts

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  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Dermatology (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Vascular Medicine (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Pulmonology (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -' Organization International Bureau (43) International Publication Date .... ....r .„,„1 8 June 2017 (08.06.2017) IN I PO I PCT ID Hit (10) WO International 2017/096285 111111111111111111IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIMIIIIIIIIIIIIIII Publication Al Number (51) International Patent Classification: Quebec H8R 2M4 (CA). NGUYEN, Justin, K.; 45 Cross C12M 1/00 (2006.01) C12M 3/00 (2006.01) Street, Apt. 24, Medford, Massachusetts 02115 (US). C12M 1/34 (2006.01) C12M 3/04 (2006.01) C12M 1/42 (2006.01) C12Q 1/02 (2006.01) (74) Agents: RESNICK, David, S. et al.; Nixon Peabody LLP, 70 W. Madison St., Suite 3500, Chicago, Illinois 60602- (21) International Application Number: 4224 (US). PCT/US2016/064798 (81) Designated States (unless otherwise indicated, for every (22) International Filing Date: kind of national protection available): AE, AG, AL, AM, 2 December 2016 (02.12.2016) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (25) Filing Language: English DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (26) Publication Language: English HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (30) Priority Data: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 62/263,225 4 December 2015 (04.12.2015) US OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (71) Applicant: PRESIDENT AND FELLOWS OF HAR- SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, YARD COLLEGE [US/US]; 17 Quincy Street, Cam- TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, bridge, Massachusetts 02138 (US). ZW. (72) Inventors: VARONE, Antonio; 865 Boylston Street, (84) Designated States (unless otherwise indicated, for every Newton, Massachusetts 02461 (US). WEN, Norman; 865 kind of regional protection available): ARIPO (BW, GH, Boylston St., Apt. 5, Newton, Massachusetts 02461 (US). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, LEVNER, Daniel; 55 Sewall Avenue, Apt. 5C, Brookline, Massachusetts 02446 (US). NOVAK, Richard; 42 Rock- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Street, Unit 4, Jamaica Plain, Massachusetts 02130 DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, view LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (US). MCPARTLIN, Lori; 49 Twombley Ave., North SM, TR), OAPI BJ, CF, CG, CI, CM, GA, GN, GQ, (BF, Billerica, Massachusetts 01862 (US). INGBER, Donald, GW, KM, ML, MR, NE, SN, TD, TG). E.; 162 W. Canton St., Boston, Massachusetts 02118 (US). CHOE, Youngjae; 35 Rose St., Somerville, Massachusetts 02143 (US). LENG, Lian; 8985 LaSalle Blvd., LaSalle, [Continued on next page] (54) Title: OPEN-TOP MICROFLUIDIC DEVICES AND METHODS FOR SIMULATING A FUNCTION OF A TISSUE 300 334 0 320 342 -344 340 11 - - - - - - _- - . - -, _- 348 .4t 325 \ 305 GC \. 3 36 N Cr\ FIG. 3 0 IN 1-1 (57) : A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first N structure defmes a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defmes a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The C membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber. WO 2017/096285 Al MIDEDItitiDEIRDEFEDIECHHOHERUDIMIE Declarations under Rule 4.17: Published: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii)) — with international search report (Art 21(3)) — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii))
SG11201804706UA 2015-12-04 2016-12-02 Open-top microfluidic devices and methods for simulating a function of a tissue SG11201804706UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562263225P 2015-12-04 2015-12-04
PCT/US2016/064798 WO2017096285A1 (en) 2015-12-04 2016-12-02 Open-top microfluidic devices and methods for simulating a function of a tissue

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SG11201804706UA true SG11201804706UA (en) 2018-07-30

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US (4) US11505773B2 (en)
EP (1) EP3383992A4 (en)
JP (3) JP6876696B2 (en)
KR (1) KR20180094941A (en)
AU (3) AU2016364932B2 (en)
CA (1) CA3013313A1 (en)
GB (2) GB2595081B (en)
SG (1) SG11201804706UA (en)
WO (1) WO2017096285A1 (en)

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