SG11201506754YA - Electrophoretic separation devices and methods for using the same - Google Patents
Electrophoretic separation devices and methods for using the sameInfo
- Publication number
- SG11201506754YA SG11201506754YA SG11201506754YA SG11201506754YA SG11201506754YA SG 11201506754Y A SG11201506754Y A SG 11201506754YA SG 11201506754Y A SG11201506754Y A SG 11201506754YA SG 11201506754Y A SG11201506754Y A SG 11201506754YA SG 11201506754Y A SG11201506754Y A SG 11201506754YA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- same
- separation devices
- electrophoretic separation
- electrophoretic
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/561—Immunoelectrophoresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0621—Control of the sequence of chambers filled or emptied
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361774519P | 2013-03-07 | 2013-03-07 | |
US201361805414P | 2013-03-26 | 2013-03-26 | |
US201361866396P | 2013-08-15 | 2013-08-15 | |
PCT/US2014/021399 WO2014138475A1 (en) | 2013-03-07 | 2014-03-06 | Electrophoretic separation devices and methods for using the same |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201506754YA true SG11201506754YA (en) | 2015-09-29 |
Family
ID=51491977
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201506754YA SG11201506754YA (en) | 2013-03-07 | 2014-03-06 | Electrophoretic separation devices and methods for using the same |
Country Status (7)
Country | Link |
---|---|
US (1) | US10267795B2 (en) |
EP (2) | EP2965083B1 (en) |
JP (1) | JP6317771B2 (en) |
CN (1) | CN105408750B (en) |
GB (1) | GB2517864B (en) |
SG (1) | SG11201506754YA (en) |
WO (1) | WO2014138475A1 (en) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
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US9108195B2 (en) | 2011-06-24 | 2015-08-18 | The Regents Of The University Of California | Microfluidic devices and methods for separating and detecting constituents in a fluid sample |
EP3149476B1 (en) * | 2014-05-30 | 2022-02-16 | The Regents of The University of California | Subcellular western blotting of single cells |
CN107209198B (en) | 2015-04-30 | 2019-07-19 | 惠普发展公司,有限责任合伙企业 | Target component positioning and discharge |
EP3298395B1 (en) | 2015-05-20 | 2020-07-29 | ProteinSimple | System and methods for electrophoretic separation and analysis of analytes |
US10768141B2 (en) | 2015-09-11 | 2020-09-08 | The Regents Of The University Of California | Isoelectric focusing arrays and methods of use thereof |
DK3472351T3 (en) | 2016-06-15 | 2020-11-09 | Univ Muenchen Ludwig Maximilians | Single molecule detection or quantification using DNA nanotechnology |
CN109126911A (en) * | 2017-06-15 | 2019-01-04 | 南京巴傲得生物科技有限公司 | A kind of micro-fluid chip in WB experiment |
KR101900368B1 (en) | 2017-08-29 | 2018-09-20 | 국립암센터 | Precast gel, electric current providing module, divice for electrophoresis and western blotting and control method thereof |
JP2019052932A (en) * | 2017-09-14 | 2019-04-04 | 国立研究開発法人理化学研究所 | Data analyzer, program and recording medium, and data analysis method |
WO2019212974A1 (en) * | 2018-04-30 | 2019-11-07 | The Regents Of The University Of California | Tissue projection electrophoretic separation of protein |
WO2021199031A1 (en) * | 2020-03-29 | 2021-10-07 | Tsc-Analytics Ltd. | Method of analyzing a single-cell western blot chip |
US11237164B2 (en) * | 2020-04-15 | 2022-02-01 | ProteinSimple | Method for measurement of total protein content and detection of protein via immunoassay in a microfluidic device |
CN111733056B (en) * | 2020-06-18 | 2022-09-27 | 水熊健康科技(南通)有限公司 | Micro-fluidic chip integrating circulating tumor cell separation and single-cell immunoblotting |
EP3978914A1 (en) * | 2020-09-30 | 2022-04-06 | iCare Diagnostics International Co. Ltd. | Electrode, ph measuring device, and electrophoresis detection system |
WO2022159717A1 (en) * | 2021-01-21 | 2022-07-28 | ProteinSimple | Method for enhancing signals associated with electrophoretically separated analytes using post-electrophoresis treatment |
WO2023194510A1 (en) * | 2022-04-05 | 2023-10-12 | Vysens B.V. | Method and device for depositing a compound in a gradient on a sensor surface |
Family Cites Families (44)
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US3674678A (en) * | 1970-10-28 | 1972-07-04 | Millipore Corp | Electrophoretic apparatus |
EP0425485B1 (en) | 1988-07-22 | 2000-10-04 | SurModics, Inc. | Preparation of polymeric surfaces |
US5006473A (en) * | 1988-08-09 | 1991-04-09 | Abbott Laboratories | Electrophoresis method using vesicles |
US5061336A (en) * | 1989-05-01 | 1991-10-29 | Soane Technologies, Inc. | Gel casting method and apparatus |
US6071396A (en) * | 1994-12-15 | 2000-06-06 | University College London | Gel-matrix electrophoresis |
US5785835A (en) * | 1996-04-12 | 1998-07-28 | One Lambda | Electrophoresis method and devices |
US6632619B1 (en) * | 1997-05-16 | 2003-10-14 | The Governors Of The University Of Alberta | Microfluidic system and methods of use |
US6136172A (en) * | 1997-06-30 | 2000-10-24 | Visible Genetics Inc. | Gel-forming insert for electrophoresis gels |
WO1999022228A1 (en) * | 1997-10-24 | 1999-05-06 | Northeastern University | A multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis |
US6024854A (en) * | 1998-06-11 | 2000-02-15 | Visible Genetics, Inc. | Method and apparatus for improving electrophoresis resolution |
US6818112B2 (en) | 1999-04-20 | 2004-11-16 | Target Discovery, Inc. | Protein separation via multidimensional electrophoresis |
US6664047B1 (en) * | 1999-04-30 | 2003-12-16 | Molecular Probes, Inc. | Aza-benzazolium containing cyanine dyes |
US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
US6905816B2 (en) | 2000-11-27 | 2005-06-14 | Intelligent Medical Devices, Inc. | Clinically intelligent diagnostic devices and methods |
CA2428568A1 (en) | 2000-11-28 | 2002-06-06 | Nanogen, Inc. | Microtiter plate format device and methods for separating differently charged molecules using an electric field |
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JP2006512092A (en) | 2002-12-30 | 2006-04-13 | ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア | Method and apparatus for pathogen detection and analysis |
US20050003521A1 (en) | 2003-03-11 | 2005-01-06 | O'connor David | Addressable microarray device, methods of making, and uses thereof |
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WO2009122446A1 (en) * | 2008-03-31 | 2009-10-08 | Council Of Scientific & Industrial Research | An electrophoretic device for separation of charged molecules using a petri dish |
GB2468226B (en) * | 2008-04-03 | 2011-06-01 | Univ California | Ex-vivo multi dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers |
WO2009134768A1 (en) * | 2008-05-01 | 2009-11-05 | Massachusetts Institute Of Technology (Mit) | Devices and processes for analyzing nucleic acid damage and repair using electrophoresis |
EP2370814B1 (en) | 2008-12-04 | 2015-04-29 | Massachusetts Institute of Technology | Method for diagnosing allergic reactions |
US8569046B2 (en) | 2009-02-20 | 2013-10-29 | Massachusetts Institute Of Technology | Microarray with microchannels |
CN102439460B (en) | 2009-05-19 | 2015-03-25 | 加利福尼亚大学董事会 | Multi-directional microfluidic devices and methods |
FR2950629B1 (en) * | 2009-09-25 | 2013-12-06 | Commissariat Energie Atomique | FLUORESCENT, POLYMERIZABLE COMPOUNDS, 7-HYDROXYCOUMARIN, THEIR PREPARATION, FLUORESCENT POLYMERS THEREOF AND CHEMICAL SENSORS COMPRISING SAME |
JP5959516B2 (en) | 2010-08-18 | 2016-08-02 | ライフ テクノロジーズ コーポレーション | Microwell chemical coating method for electrochemical detection devices |
JP6067572B2 (en) | 2010-12-03 | 2017-01-25 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Protein regeneration microfluidic device and method of making and using the same |
US9108195B2 (en) | 2011-06-24 | 2015-08-18 | The Regents Of The University Of California | Microfluidic devices and methods for separating and detecting constituents in a fluid sample |
US9095852B2 (en) | 2011-08-22 | 2015-08-04 | The Regents Of The University Of California | Multilayer high density microwells |
-
2014
- 2014-03-06 SG SG11201506754YA patent/SG11201506754YA/en unknown
- 2014-03-06 EP EP14760130.6A patent/EP2965083B1/en active Active
- 2014-03-06 JP JP2015561678A patent/JP6317771B2/en active Active
- 2014-03-06 GB GB1422042.0A patent/GB2517864B/en active Active
- 2014-03-06 CN CN201480007599.XA patent/CN105408750B/en active Active
- 2014-03-06 EP EP19175755.8A patent/EP3591404A1/en active Pending
- 2014-03-06 US US14/650,520 patent/US10267795B2/en active Active
- 2014-03-06 WO PCT/US2014/021399 patent/WO2014138475A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
GB2517864B (en) | 2015-12-09 |
JP2016517509A (en) | 2016-06-16 |
JP6317771B2 (en) | 2018-04-25 |
CN105408750B (en) | 2018-06-15 |
EP2965083A1 (en) | 2016-01-13 |
EP3591404A1 (en) | 2020-01-08 |
GB2517864A (en) | 2015-03-04 |
WO2014138475A1 (en) | 2014-09-12 |
US20150316547A1 (en) | 2015-11-05 |
US10267795B2 (en) | 2019-04-23 |
EP2965083A4 (en) | 2016-10-19 |
CN105408750A (en) | 2016-03-16 |
EP2965083B1 (en) | 2019-07-03 |
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