SG11201402410RA - Protein purification using bis-tris buffer - Google Patents

Protein purification using bis-tris buffer

Info

Publication number
SG11201402410RA
SG11201402410RA SG11201402410RA SG11201402410RA SG11201402410RA SG 11201402410R A SG11201402410R A SG 11201402410RA SG 11201402410R A SG11201402410R A SG 11201402410RA SG 11201402410R A SG11201402410R A SG 11201402410RA SG 11201402410R A SG11201402410R A SG 11201402410RA
Authority
SG
Singapore
Prior art keywords
bis
tris buffer
protein purification
tris
purification
Prior art date
Application number
SG11201402410RA
Other languages
English (en)
Inventor
Didier Duthe
Laure Landric-Burtin
Benoit Mothes
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of SG11201402410RA publication Critical patent/SG11201402410RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
SG11201402410RA 2011-11-23 2012-05-23 Protein purification using bis-tris buffer SG11201402410RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2011/070768 WO2013075740A1 (en) 2011-11-23 2011-11-23 Antibody purification method
PCT/EP2012/059528 WO2013075849A1 (en) 2011-11-23 2012-05-23 Protein purification using bis-tris buffer

Publications (1)

Publication Number Publication Date
SG11201402410RA true SG11201402410RA (en) 2014-06-27

Family

ID=46125462

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201402410RA SG11201402410RA (en) 2011-11-23 2012-05-23 Protein purification using bis-tris buffer

Country Status (14)

Country Link
US (2) US10131714B2 (zh)
JP (2) JP6437311B2 (zh)
KR (1) KR102048598B1 (zh)
CN (1) CN104039808B (zh)
AR (1) AR086525A1 (zh)
AU (2) AU2012342865A1 (zh)
BR (1) BR112014012275A2 (zh)
CA (1) CA2855491A1 (zh)
IL (1) IL232558B (zh)
MX (1) MX364908B (zh)
RU (1) RU2603347C2 (zh)
SG (1) SG11201402410RA (zh)
TW (1) TWI643865B (zh)
WO (2) WO2013075740A1 (zh)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
TWI675041B (zh) * 2013-05-06 2019-10-21 法商賽諾菲公司 用於純化抗體之連續多步驟方法
TWI596107B (zh) 2013-06-25 2017-08-21 卡地拉保健有限公司 單株抗體之新穎純化方法
CN103480346B (zh) * 2013-10-08 2016-04-06 天津工业大学 一种含大环化合物的新型亲和分离材质制备方法及应用
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
EP3092049A1 (en) 2014-01-08 2016-11-16 Flodesign Sonics Inc. Acoustophoresis device with dual acoustophoretic chamber
US10329323B2 (en) 2014-07-25 2019-06-25 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Method for purifying antibodies using PBS
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2016106291A1 (en) * 2014-12-22 2016-06-30 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
CN109071613A (zh) 2016-05-11 2018-12-21 通用电气医疗集团生物工艺研发股份公司 分离基质
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US11753438B2 (en) 2016-05-11 2023-09-12 Cytiva Bioprocess R&D Ab Method of cleaning and/or sanitizing a separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
EP3615070A1 (en) 2017-04-26 2020-03-04 Bristol-Myers Squibb Company Methods of antibody production that minimize disulfide bond reduction
CN114900773A (zh) 2017-12-14 2022-08-12 弗洛设计声能学公司 声泳***及其操作方法、控制声换能器及声学***的方法
US11603527B2 (en) * 2017-12-27 2023-03-14 Global Life Sciences Solutions Usa Llc Method and kit for viral vector isolation
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
EP3791172B1 (en) * 2018-05-08 2023-06-28 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
EP3636657A1 (en) 2018-10-08 2020-04-15 Ablynx N.V. Chromatography-free antibody purification method
CN113845562A (zh) * 2020-06-28 2021-12-28 佛山汉腾生物科技有限公司 目的蛋白纯化方法
WO2022234412A1 (en) 2021-05-03 2022-11-10 Lupin Limited A process for purification of fc-fusion proteins
US20230240961A1 (en) * 2022-01-28 2023-08-03 Praj Industries Limited Process for the preparation of antimalassezia powder

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0391934A1 (en) * 1987-10-23 1990-10-17 Schering Corporation Method of purifying protein
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5891741A (en) * 1997-05-16 1999-04-06 Coulter International Corp. Antibody-aminodextran-phycobiliprotein conjugates
CN1136317C (zh) * 1999-03-12 2004-01-28 中国人民解放军第四军医大学 单克隆抗体双段和单段的制备方法
US20020009445A1 (en) 2000-07-12 2002-01-24 Yansheng Du Human beta-amyloid antibody and use thereof for treatment of alzheimer's disease
US6602855B2 (en) * 2001-04-30 2003-08-05 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1449 daltons
ES2415358T3 (es) * 2004-04-21 2013-07-25 The Brigham And Women's Hospital, Inc. Péptidos de fijación a la poli-N-acetil glucosamina (PNAG/DPNAG) y procedimientos para el uso de los mismos
AU2005296351B2 (en) * 2004-10-21 2010-12-02 Cytiva Bioprocess R&D Ab A method of antibody purification
CN112480257A (zh) 2005-03-23 2021-03-12 根马布股份公司 用于治疗多发性骨髓瘤的cd38抗体
CN1814775A (zh) * 2005-12-06 2006-08-09 中国海洋大学 一种抗病毒活性寡聚肽的制备方法
EP1914242A1 (en) * 2006-10-19 2008-04-23 Sanofi-Aventis Novel anti-CD38 antibodies for the treatment of cancer
AU2008274195B2 (en) 2007-07-11 2013-09-05 Novo Nordisk A/S Purification of factor VIII using a mixed-mode or multimodal resin
HUE033825T2 (en) 2007-11-16 2018-01-29 Univ Rockefeller Beta-amyloid protein protofibrillum-specific antibodies
MX2010009398A (es) * 2008-02-29 2010-11-12 Biogen Idec Inc Proteinas de fusion de inmunoglobulinas purificadas y los metodos para su purificacion.
US20110237781A1 (en) 2008-07-18 2011-09-29 Talecris Biotherapeutics, Inc. Method of preparing alpha-1 proteinase inhibitor
EP2360183B1 (en) * 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antibody purification method by mixed mode chromatography utilizing an arginine-containing loading solution
EP2376523A4 (en) 2009-01-13 2012-11-28 Ge Healthcare Bio Sciences Ab Precipitation of biomolecules with negatively charged polymers
KR101224468B1 (ko) 2009-05-20 2013-01-23 주식회사 파멥신 신규한 형태의 이중표적항체 및 그 용도
CN102574911B (zh) 2009-08-07 2017-06-06 Emd密理博公司 从样品的一或多种杂质中纯化靶蛋白的方法
US9527010B2 (en) 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
EP2490780A4 (en) 2009-10-20 2014-04-09 Merck Sharp & Dohme USE OF MIXED MODE CHROMATOGRAPHY FOR CAPTURE AND PURIFICATION OF BASIC ANTIBODY PRODUCTS
ES2534936T3 (es) * 2010-08-02 2015-04-30 Ratiopharm Gmbh Método para producir y purificar una sialiltransferasa soluble activa
WO2012125735A1 (en) 2011-03-15 2012-09-20 Abott Laboratories An integrated approach to the isolation and purification of antibodies
EP2691411B1 (en) * 2011-03-29 2020-02-26 GlaxoSmithKline LLC Buffer system for protein purification
US8445284B2 (en) * 2011-06-17 2013-05-21 Constitution Medical, Inc. Fixative and staining solutions
WO2013028330A2 (en) 2011-08-19 2013-02-28 Emd Millipore Corporation Methods of reducing level of one of more impurities in a sample during protein purification
EP4088736A1 (en) 2011-10-25 2022-11-16 Prothena Biosciences Limited Humanized anti-amyloid antibody formulations and methods
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method

Also Published As

Publication number Publication date
TW201321402A (zh) 2013-06-01
US20190135942A1 (en) 2019-05-09
CA2855491A1 (en) 2013-05-30
IL232558B (en) 2018-08-30
WO2013075849A1 (en) 2013-05-30
IL232558A0 (en) 2014-06-30
US20140323698A1 (en) 2014-10-30
AR086525A1 (es) 2013-12-18
AU2012342865A1 (en) 2014-06-19
JP6437311B2 (ja) 2018-12-12
WO2013075740A1 (en) 2013-05-30
CN104039808B (zh) 2019-04-19
MX2014006284A (es) 2014-10-24
BR112014012275A2 (pt) 2017-05-30
JP2017095505A (ja) 2017-06-01
AU2017245478B2 (en) 2019-05-16
MX364908B (es) 2019-05-13
JP2015500798A (ja) 2015-01-08
AU2017245478A1 (en) 2017-11-02
KR102048598B1 (ko) 2019-11-25
RU2603347C2 (ru) 2016-11-27
KR20140094013A (ko) 2014-07-29
US10131714B2 (en) 2018-11-20
TWI643865B (zh) 2018-12-11
RU2014125063A (ru) 2015-12-27
CN104039808A (zh) 2014-09-10

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