SG10201906070XA - Compositions and methods for high efficiency in vivo genome editing - Google Patents
Compositions and methods for high efficiency in vivo genome editingInfo
- Publication number
- SG10201906070XA SG10201906070XA SG10201906070XA SG10201906070XA SG10201906070XA SG 10201906070X A SG10201906070X A SG 10201906070XA SG 10201906070X A SG10201906070X A SG 10201906070XA SG 10201906070X A SG10201906070X A SG 10201906070XA SG 10201906070X A SG10201906070X A SG 10201906070XA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- high efficiency
- genome editing
- compositions
- vivo genome
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines. No suitable figure
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462099014P | 2014-12-31 | 2014-12-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201906070XA true SG10201906070XA (en) | 2019-08-27 |
Family
ID=56285162
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201906070XA SG10201906070XA (en) | 2014-12-31 | 2015-12-31 | Compositions and methods for high efficiency in vivo genome editing |
SG11201704272YA SG11201704272YA (en) | 2014-12-31 | 2015-12-31 | Compositions and methods for high efficiency in vivo genome editing |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201704272YA SG11201704272YA (en) | 2014-12-31 | 2015-12-31 | Compositions and methods for high efficiency in vivo genome editing |
Country Status (8)
Country | Link |
---|---|
US (2) | US11339399B2 (en) |
EP (1) | EP3240889A4 (en) |
JP (2) | JP2018500037A (en) |
AU (1) | AU2015373893B2 (en) |
CA (1) | CA2972454A1 (en) |
IL (1) | IL252970B (en) |
SG (2) | SG10201906070XA (en) |
WO (1) | WO2016109840A2 (en) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
WO2017011707A1 (en) * | 2015-07-14 | 2017-01-19 | Synthetic Genomics, Inc | Microorganisms having increased lipid productivity |
JP7409773B2 (en) | 2015-07-31 | 2024-01-09 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | Modified cells and methods of treatment |
EP3344766B8 (en) * | 2015-09-01 | 2021-03-17 | Dana-Farber Cancer Institute, Inc. | Systems and methods for selection of grna targeting strands for cas9 localization |
CN108699116A (en) | 2015-10-23 | 2018-10-23 | 哈佛大学的校长及成员们 | The CAS9 albumen of evolution for gene editing |
US10041079B2 (en) | 2015-11-30 | 2018-08-07 | Synthetic Genomics, Inc. | Compositions and methods for expressing genes in algae |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
WO2017100376A2 (en) | 2015-12-07 | 2017-06-15 | Zymergen, Inc. | Promoters from corynebacterium glutamicum |
US20170191094A1 (en) * | 2015-12-31 | 2017-07-06 | Synthetic Genomics, Inc. | Expression of type i fatty acid synthase genes in eukaryotic algae |
KR102345898B1 (en) | 2016-06-30 | 2022-01-03 | 지머젠 인코포레이티드 | Methods for generating glucose permeabilization enzyme libraries and uses thereof |
KR102345899B1 (en) | 2016-06-30 | 2021-12-31 | 지머젠 인코포레이티드 | Methods for generating bacterial hemoglobin libraries and uses thereof |
BR112019000430A2 (en) * | 2016-07-13 | 2019-07-09 | Dsm Ip Assets Bv | crispr-cas system for an algae host cell |
WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
EP3519570B1 (en) * | 2016-09-29 | 2022-06-08 | F. Hoffmann-La Roche AG | Method to analyze and optimize gene editing modules and delivery approaches |
CA3039928A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
WO2018075664A1 (en) | 2016-10-18 | 2018-04-26 | Regents Of The University Of Minnesota | Tumor infiltrating lymphocytes and methods of therapy |
GB201618414D0 (en) * | 2016-11-01 | 2016-12-14 | Patterson James | Regulated cell lines and methods of use thereof |
GB201618507D0 (en) * | 2016-11-02 | 2016-12-14 | Stichting Voor De Technische Wetenschappen And Wageningen Univ | Microbial genome editing |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
WO2018220582A1 (en) * | 2017-05-31 | 2018-12-06 | Tropic Biosciences UK Limited | Methods of selecting cells comprising genome editing events |
EP3645021A4 (en) | 2017-06-30 | 2021-04-21 | Intima Bioscience, Inc. | Adeno-associated viral vectors for gene therapy |
JP2020534795A (en) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Methods and Compositions for Evolving Base Editing Factors Using Phage-Supported Continuous Evolution (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
WO2019113463A1 (en) | 2017-12-08 | 2019-06-13 | Synthetic Genomics, Inc. | Improving algal lipid productivity via genetic modification of a tpr domain containing protein |
CA3084828A1 (en) * | 2017-12-29 | 2019-07-04 | Synthetic Genomics, Inc. | Genetic modulation of photosynthetic organisms for improved growth |
KR20210045360A (en) * | 2018-05-16 | 2021-04-26 | 신테고 코포레이션 | Methods and systems for guide RNA design and use |
WO2020023353A1 (en) * | 2018-07-23 | 2020-01-30 | Synthetic Genomics, Inc. | Microorganisms having increased lipid production, and compositions and methods of making and using the same |
EP3862430A4 (en) * | 2018-10-04 | 2022-06-15 | Kaneka Corporation | Dna construct to be used in genome editing of plant |
KR20210091167A (en) * | 2018-10-16 | 2021-07-21 | 블루알렐, 엘엘씨 | Methods for targeted insertion of DNA in genes |
US11162106B2 (en) * | 2018-12-19 | 2021-11-02 | Synthetic Genomics, Inc. | Inducible expression of genes in algae |
WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
KR20210148269A (en) * | 2019-04-05 | 2021-12-07 | 다니스코 유에스 인크. | Methods for integrating donor DNA sequences into the Bacillus genome using linear recombinant DNA constructs and compositions thereof |
CN110331169A (en) * | 2019-07-05 | 2019-10-15 | 亢庆铮 | It is a kind of efficiently quickly in screening-gene regulatory region functional site method and application |
JP7428459B2 (en) * | 2019-07-08 | 2024-02-06 | 株式会社ユーグレナ | Euglena genome modification method and Euglena breeding method |
WO2021085580A1 (en) * | 2019-10-31 | 2021-05-06 | 国立研究開発法人理化学研究所 | Polynucleotide and use for same |
DE112021002672T5 (en) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EDIT BOTH STRANDS SIMULTANEOUSLY OF A DOUBLE STRANDED NUCLEOTIDE TARGET SEQUENCE |
WO2022080234A1 (en) * | 2020-10-12 | 2022-04-21 | Dic株式会社 | Method for modifying genome of algae belonging to genus galdieria |
WO2023147058A2 (en) * | 2022-01-27 | 2023-08-03 | Asklepios Biopharmaceutical, Inc. | Compositions for treating neurological disease |
WO2023225358A1 (en) * | 2022-05-20 | 2023-11-23 | The Board Of Trustees Of The Leland Stanford Junior University | Generation and tracking of cells with precise edits |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9304200D0 (en) | 1993-03-02 | 1993-04-21 | Sandoz Ltd | Improvements in or relating to organic compounds |
US5767373A (en) | 1994-06-16 | 1998-06-16 | Novartis Finance Corporation | Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms |
US8119859B2 (en) | 2008-06-06 | 2012-02-21 | Aurora Algae, Inc. | Transformation of algal cells |
MX2013015327A (en) | 2011-06-30 | 2014-01-31 | Exxonmobil Res & Eng Co | Regulation of toxin and antitoxin genes for biological containment. |
US8709766B2 (en) | 2011-10-17 | 2014-04-29 | Colorado School Of Mines | Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana |
US8809026B2 (en) | 2011-12-27 | 2014-08-19 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
GB2537000C (en) * | 2012-05-25 | 2019-10-09 | Univ California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
US10612034B2 (en) | 2012-06-01 | 2020-04-07 | Exxonmobil Research And Engineering Company | Promoters and terminators for use in eukaryotic cells |
US9546372B2 (en) | 2012-06-12 | 2017-01-17 | Synthetic Genomics, Inc. | Regulatory elements and uses thereof |
AU2012396282B2 (en) | 2012-12-04 | 2016-05-19 | Exxonmobil Research And Engineering Company | Tetraselmis promoters and terminators for use in eukaryotic cells |
US9982272B2 (en) | 2012-12-06 | 2018-05-29 | Synthetic Genomics, Inc. | Algal mutants having a locked-in high light acclimated phenotype |
WO2014093701A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
CN105164264B (en) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
DK2921557T3 (en) | 2012-12-12 | 2016-11-07 | Broad Inst Inc | Design of systems, methods and optimized sequence manipulation guide compositions |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
CN114230675A (en) * | 2013-06-05 | 2022-03-25 | 杜克大学 | RNA-guided gene editing and gene regulation |
JP6625971B2 (en) * | 2013-06-17 | 2019-12-25 | ザ・ブロード・インスティテュート・インコーポレイテッド | Delivery, engineering and optimization of tandem guide systems, methods and compositions for array manipulation |
US9765126B2 (en) | 2013-10-04 | 2017-09-19 | Synthetic Genomics, Inc. | Compositions and methods for modulating biomass productivity |
WO2015103307A1 (en) | 2013-12-31 | 2015-07-09 | Synthetic Genomics, Inc. | Biomass productivity regulator |
CN103668472B (en) * | 2013-12-31 | 2014-12-24 | 北京大学 | Method for constructing eukaryon gene knockout library by using CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system |
JP7057786B2 (en) * | 2016-12-30 | 2022-04-20 | シンセティック ジェノミクス インコーポレーテッド | Highly productive algal mutants with reduced photosynthetic antennas |
-
2015
- 2015-12-31 CA CA2972454A patent/CA2972454A1/en active Pending
- 2015-12-31 JP JP2017534947A patent/JP2018500037A/en active Pending
- 2015-12-31 SG SG10201906070XA patent/SG10201906070XA/en unknown
- 2015-12-31 US US14/986,492 patent/US11339399B2/en active Active
- 2015-12-31 SG SG11201704272YA patent/SG11201704272YA/en unknown
- 2015-12-31 AU AU2015373893A patent/AU2015373893B2/en active Active
- 2015-12-31 WO PCT/US2015/068356 patent/WO2016109840A2/en active Application Filing
- 2015-12-31 EP EP15876386.2A patent/EP3240889A4/en active Pending
-
2017
- 2017-06-18 IL IL252970A patent/IL252970B/en unknown
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2020
- 2020-09-29 JP JP2020162773A patent/JP7046139B2/en active Active
- 2020-09-30 US US17/039,839 patent/US20210017530A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20210017530A1 (en) | 2021-01-21 |
CA2972454A1 (en) | 2016-07-07 |
US20170073695A1 (en) | 2017-03-16 |
SG11201704272YA (en) | 2017-06-29 |
EP3240889A2 (en) | 2017-11-08 |
EP3240889A4 (en) | 2018-06-20 |
JP2018500037A (en) | 2018-01-11 |
WO2016109840A3 (en) | 2016-10-13 |
JP7046139B2 (en) | 2022-04-01 |
JP2021036866A (en) | 2021-03-11 |
IL252970B (en) | 2021-07-29 |
WO2016109840A9 (en) | 2016-11-17 |
AU2015373893A1 (en) | 2017-06-15 |
IL252970A0 (en) | 2017-08-31 |
WO2016109840A2 (en) | 2016-07-07 |
AU2015373893B2 (en) | 2021-07-29 |
US11339399B2 (en) | 2022-05-24 |
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