CN113046299A - Additive for preparing islet beta cells by inducing pluripotent stem cells to differentiate directionally - Google Patents
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- CN113046299A CN113046299A CN202110297334.XA CN202110297334A CN113046299A CN 113046299 A CN113046299 A CN 113046299A CN 202110297334 A CN202110297334 A CN 202110297334A CN 113046299 A CN113046299 A CN 113046299A
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Abstract
The additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells comprises a JNK inhibitor, a hedgehog pathway antagonist, an EGF signal factor, an FGF signal factor, a TGF-beta inhibitor, a WNT signal pathway activator, a Notch signal pathway inhibitor, a small molecular compound and trace elements, the proportion of the additive in different stages of cell culture is different, and each component can perform the action in a staged directed manner, so that the directed differentiation of the pluripotent stem cells into the islet beta cells can be efficiently induced.
Description
Technical Field
The invention belongs to the technical field of cell engineering, and particularly relates to an additive for preparing islet beta cells by inducing pluripotent stem cells to differentiate directionally.
Background
Diabetes Mellitus (DM) is a metabolic disorder characterized clinically by elevated blood sugar, and its main pathogenesis is the reduction of insulin secretion and its utilization disorder. At present, diabetes mellitus becomes the third disease which is a serious threat to human health and influences the quality of life of people after tumors and cardiovascular and cerebrovascular diseases. Recent reports from the World Health Organization (WHO) in 2016 show that 4.22 million people worldwide have diabetes, while the number of diabetes patients in china exceeds 1 million people, which is the first of the world.
There are two types of diabetes: type I diabetes (T1D), which accounts for 5-10% of the number of diseases, is an autoimmune disease caused by the selective destruction of islet beta cells; type II diabetes (T2D), accounting for over 90% of the number of diseases, is a disease that results from insulin resistance in peripheral organs, including the liver, fat and muscle. Diabetes patients show loss of insulin-producing cells in vivo, i.e., islet beta cells, or decreased insulin utilization, and the current treatment means is exogenous insulin injection to control blood sugar balance in vivo. Although this method can effectively control the disease progression, the long-term insulin injection can not stably maintain the physiological balance of blood sugar in the body, which results in some high-risk diseases and complications. Vascular disorders caused by complications such as hypoglycemia and hyperglycemia can cause cardiovascular, renal or neurological diseases. Thus, there is a need for a treatment strategy for diabetes that can reduce or even eliminate long-term complications.
One possible approach is to implant human islets into a patient for the treatment of diabetes. The method can well control blood sugar balance in human body, avoid dependence and pain of long-term insulin injection, and further improve the whole life quality. However, the lack of donor islets, donor-recipient islet immune rejection, and variability in islet preparation limit, making islet transplantation regimens for treating diabetes not universally applicable. Therefore, research is currently focused on alternatives that can obtain large numbers of insulin-producing secretory cells.
One such method is the direct differentiation of embryonic stem cells (hESCs) into insulin-secreting cells. However, due to ethical limitations, the currently available embryonic stem cell lines are limited and are not suitable for future clinical cell therapy. Subsequent studies focused primarily on the differentiation of pancreatic endocrine cells using other types of pluripotent stem cells, which appeared to be achieved using a combination of signaling molecules and their associated inhibitors/agonists, typically following 6-7 successive differentiation stages, respectively: definitive Endoderm (DE), primitive embryonic gut tube (primtivoguttube), posterior foregut (posteroorforegut), Pancreatic Endoderm (PE), endocrine precursor cells (EP), and β -like early cells, and further differentiated into mature pancreatic islet β -like cells. However, these differentiation methods still produce immature beta cells, which express only a limited and unstable variety of hormones and have limited insulin content, and thus cannot be used for transplantation therapy of diabetic patients.
In conclusion, since insulin injection causes various complications, islet transplantation sources are limited, and the existing islet beta cell differentiation scheme is immature, has a small amount or does not have the function of GSIS, so that the treatment of diabetes is greatly limited, and a new islet beta cell differentiation method and a new diabetes treatment strategy are urgently needed to be solved.
Disclosure of Invention
1. Technical problem to be solved by the invention
The purpose of the present invention is to solve the problems of a small number of islet beta cells and an incomplete function of islet beta cells produced by the differentiation of islet beta cells in the prior art.
2. Technical scheme
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells comprises a JNK inhibitor, a hedgehog pathway antagonist, an EGF signal factor, an FGF signal factor, a TGF-beta inhibitor, a WNT signal pathway activator, a Notch signal pathway inhibitor, a small molecular compound and trace elements, and the proportion of the additive in different stages of cell culture is different.
Preferably, the JNK inhibitor is CC-930, the hedgehog pathway antagonist is N-acetylcysteine or cyclopamine, the EGF signaling factor is EGF, the FGF signaling factor is FGF10, the TGF-beta superfamily factor is ActivinA, the TGF-beta inhibitor is ALK5iII, the WNT signaling pathway activator is BML-284 or WNT3a, and the Notch signaling pathway inhibitor is FLI-06 or DAPT.
Preferably, the small molecule compound comprises a keratin growth factor, a protein kinase C activator, a ROCK1 inhibitor, a Sirt1 inhibitor, a C-Met inhibitor, a thyroid hormone, and retinoic acid (Retinoic acid).
Preferably, the keratin growth factor is KGF, the protein kinase C activator is TPB, the ROCK1 inhibitor is Y27632, the Sirt1 inhibitor is Nicotinamide, the C-Met inhibitor is BMS-777607, and the thyroid hormone is T3.
Preferably, the composite also comprises an ALK4/5/7 inhibitor, a CDK5 inhibitor, an L-type calcium channel (LTCC) activator and a Hippo signal channel effector inhibitor, wherein the ALK4/5/7 inhibitor is A83-01, the CDK5 inhibitor is AT7519, the L-type calcium channel (LTCC) activator is BayK8644, and the Hippo signal channel effector inhibitor is Super-TDU 1-31.
Preferably, the additive has different proportions in different stages of cell culture, and can be specifically divided into additive A, additive B, additive C and additive D, wherein the additive A is used for inducing the differentiation of the multifunctional cell balls into the stage of definitive endoderm, the additive B is used for inducing the differentiation of the definitive endoderm into the stage of pancreatic precursor cells, the additive C is used for inducing the differentiation of the pancreatic precursor cells into the stage of pancreatic endocrine progenitor cells, and the additive D is used for inducing the differentiation of the pancreatic endocrine progenitor cells into the stage of islet beta cells.
Preferably, the additive A comprises TGF-beta factor, WNT signal pathway activator and JNK signal inhibitor, wherein the concentration of the TGF-beta factor is 30-100 ng/mL; the concentration of WNT signal pathway activator is 1-5 μ M; the concentration of JNK signal inhibitor is 0.5-5 μ M.
Preferably, the additive B comprises EGF signaling factor, retinoic acid, keratin growth factor, protein kinase C activator and Sirt1 inhibitor, wherein the concentration of the EGF signaling factor is 2-20 ng/mL; retinoic acid (retinoic acid) concentration of 1-5 μ M; the concentration of the keratin growth factor is 20-40 ng/mL; the concentration of the protein kinase C activator is 100-300 nM; the concentration of Sirt1 inhibitor was 10-40. mu.M.
Preferably, the additive C comprises TGF- β inhibitors, hedgehog signaling pathway inhibitors, Notch signaling pathway inhibitors, FGF signaling factors, and thyroid hormones; wherein the concentration of the TGF-beta inhibitor is 5-20 mu M; the concentration of the hedgehog signal pathway inhibitor is 10-50 ng/mL; the concentration of Notch signaling pathway inhibitor is 0.2-1 μ M; the concentration of FGF signal factor is 5-50 ng/ml; the concentration of thyroid hormone is 0.1-1 μ M.
Preferably, the additive D comprises a TGF-beta inhibitor, a C-Met inhibitor and trace elements, wherein the concentration of the TGF-beta inhibitor is 5-20 mu M; the concentration of the C-Met inhibitor is 20-100 nM.
The concentration of the additional factors in the above-mentioned additives A to D is in a range of concentration that can induce successful differentiation into the cell type at this stage, and no factors below or above this range of concentration can successfully induce or obtain poor quality of the final cell (e.g., low induction efficiency, small amount of final product, incomplete function, etc.).
The use method of the additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells specifically comprises the following steps:
s100, preparing a culture medium, namely adding an additive A, an additive B, an additive C and an additive D into the same culture medium respectively to obtain a culture medium A, a culture medium B, a culture medium C and a culture medium D;
s200, preparing an induced multifunctional cell ball;
s300, performing primary differentiation, namely adding a culture medium A into the induced multifunctional cell balls, and performing directional differentiation culture to obtain definitive endoderm cells;
s400, performing secondary differentiation, namely adding a culture medium B into the definitive endoderm cells, and performing induced differentiation to obtain pancreatic precursor cells;
s500, performing tertiary differentiation, namely adding a culture medium C into the pancreatic precursor cells, and performing induced differentiation to obtain pancreatic endocrine progenitor cells;
s600, differentiating for four times, adding a culture medium D into the pancreatic endocrine progenitor cells, and inducing and differentiating to obtain the required islet beta cells.
Preferably, the step S200 of preparing the multifunctional cell-inducing pellet is to digest the adherent cultured multifunctional stem cells into small cell blocks, then resuspend the small cell blocks in a complete mTeSR-1 culture medium containing ROCK1 inhibitor, and resuspend the whole mTeSR-1 culture medium in a 0.2 × 10 culture medium6/cm2The cell density of the cell is inoculated in an ultra-low adsorption six-hole plate, the cell is cultured for 24 hours under the conditions of 37 ℃ and 5 percent oxygen until the cell becomes a regular pellet, and then 3D suspension culture is carried out for 2-3 days, so as to obtain the induced multifunctional cell pellet.
Preferably, the step S300 is to add the culture medium A into the induced multifunctional cell ball and add the culture medium A into the induced multifunctional cell ball at 37 ℃ and 5% CO2Culturing for 3 days to obtain definitive endoderm cells, and replacing culture medium A every 1 day during culture.
Preferably, step S400 is to add culture medium B to definitive endoderm cells at 37 deg.C and 5% CO2The pancreatic precursor cells were obtained by further culturing for 5 days under the conditions, and the culture medium B was changed every 1 day during the culture.
Preferably, the step S500 is to add the culture medium C to the pancreatic precursor cells, and to add the culture medium C to the pancreatic precursor cells at 37 ℃ and 5% CO2The pancreatic endocrine progenitor cells were obtained by further culturing for 7 days under the conditions, and the medium C was replaced every 1 day during the culture.
Preferably, the step S600 is to add the culture medium D to the pancreatic endocrine progenitor cells, and 5% CO at 37 ℃2Under the condition, the islet beta cells are obtained after 7 days of culture, and the culture medium D is replaced every 2-4 days during the continuous culture period.
3. Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
the additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells comprises a JNK inhibitor, a hedgehog pathway antagonist, an EGF signal factor, an FGF signal factor, a TGF-beta inhibitor, a WNT signal pathway activator, a Notch signal pathway inhibitor, a small molecular compound and trace elements, the proportion of the additive in different stages of cell culture is different, and each component can perform the action in a staged directed manner, so that the directed differentiation of the pluripotent stem cells into the islet beta cells can be efficiently induced.
Drawings
FIG. 1 is a photomicrograph of Induced Pluripotent Stem Cells (iPSCs) of the present invention in suspension culture; wherein, FIG. 1-A is the picture of normal iPSCs clone (adherent culture), and FIGS. 1-B, C and D are the cell ball morphology after 2h, 24h and 72h of suspension culture (10 Xmirror picture), respectively.
FIG. 2 is a photomicrograph of islet beta cells obtained using and culturing the additives of the present invention; wherein FIGS. 2-A, B, C and D are the cell pellet morphology of the four stages (DE stage, PP stage, EN stage and islet beta cell stage) respectively, cultured using the method of example 3; and FIGS. 2-A 'to D' are the cell pellet morphology (4 Xmirror images) at four stages of culture using the method of example 4 (addition of labeled molecules).
FIG. 3 is an identification of characteristic protein expression of DE cells, a key stage in the process of using the additive of the present invention to differentiate islet beta cells committed, including immunofluorescence microscopy and flow cytometry; wherein FIGS. 3-A' are the immunofluorescence pictures of the cell balls at the DE stage, including DE Marker-FOXA2 and SOX17 and the resultant pictures; FIGS. 3-B' are photographs of the DE stage single cell immunofluorescence; FIGS. 3-C' are the results of the DE-stage cytometric flow cytometry, including two markers FOXA2 and SOX17 (both immunofluorescence pictures were taken at 20 Xmirror).
FIG. 4 shows the results of immunofluorescence assay of characteristic protein expression of islet precursor cells (PP) at key stages during the process of using the additive of the present invention to differentiate islet beta cells; FIGS. 4-A "and FIGS. 4-B" are immunofluorescence staining pictures of the cell balls and single cells at the PP stage, respectively, including PDX1, NKX6.1 and the resultant pictures (all the immunofluorescence pictures are taken with 20 Xlens).
FIG. 5 is the identification of characteristic protein expression and insulin release function assay of the additive of the present invention using committed differentiated islet beta cells; wherein, FIG. 5-A is an immunofluorescence identification picture (20X microscope shoot) of islet beta cell Marker (NKX6.1, C-peptide, insulin and MAFA) and islet alpha cell Marker (glucagon); FIG. 5-B shows the results of Elisa assay of insulin content (or secretion function) of islet beta cells (two experiments Exp-1 and Exp-2); FIG. 5-C shows the results of Elisa assays of insulin secretion function (GSIS) of pancreatic islet beta cells stimulated by different concentrations of glucose.
Detailed Description
In order to facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which may be embodied in many different forms and are not limited to the embodiments described herein, but rather are provided for the purpose of providing a more thorough disclosure of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; as used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
The additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells comprises a JNK inhibitor, a hedgehog pathway antagonist, an EGF signaling factor, an FGF signaling factor, a TGF-beta inhibitor, a WNT signaling pathway activator, a Notch signaling pathway inhibitor, a small molecule compound and trace elements, and the proportion of the additive in different stages of cell culture is different.
The JNK inhibitor is CC-930, the hedgehog pathway antagonist is N-acetylcysteine or cyclopamine, the EGF signal factor is EGF, the FGF signal factor is FGF10, the TGF-beta superfamily factor is ActivinA, the TGF-beta inhibitor is ALK5iII, the WNT signal pathway activator is BML-284 or WNT3a, and the Notch signal pathway inhibitor is FLI-06 or DAPT.
The small molecule compounds include keratin growth factor, protein kinase C activator, ROCK1 inhibitor, Sirt1 inhibitor, C-Met inhibitor, thyroid hormone and retinoic acid (Retinoic acid).
The keratin growth factor is KGF, the protein kinase C activator is TPB, the ROCK1 inhibitor is Y27632, the Sirt1 inhibitor is Nicotinamide, the C-Met inhibitor is BMS-777607, and the thyroid hormone is T3.
Also comprises an ALK4/5/7 inhibitor, a CDK5 inhibitor, an L-type calcium channel (LTCC) activator and a Hippo signal channel effector inhibitor, wherein the ALK4/5/7 inhibitor is A83-01, the CDK5 inhibitor is AT7519, the L-type calcium channel (LTCC) activator is BayK8644, and the Hippo signal channel effector inhibitor is Super-TDU 1-31.
Wherein the TGF-beta superfamily factor is Activin A, the WNT signal pathway activator is BML-284 or WNT3a, and the TGF-beta superfamily factor is a key factor for endoderm lineage cell specific differentiation; the JNK signaling pathway inhibitor CC-930 can enhance ActivThe in A and WNT3a can obviously promote the efficient differentiation of pluripotent stem cells to the epiblast; retinoic Acid (RA) can promote migration to PDX1+Differentiation of pancreatic precursor cells; the Sirt1 inhibitor is Nicotinamide, and can promote the action on NKX6.1+Differentiation of pancreatic precursor cells; the combined use of epidermal growth factor and keratin growth factor can promote PDX1+And NKX6.1+Differentiation of double positive pancreatic precursor cells; the protein kinase C activator is TPB, can promote the specific differentiation to pancreatic lineage cells, has synergistic effect with RA, and can remarkably enhance PDX1+Differentiation of the cells; the hedgehog pathway antagonist is N-acetylcysteine or cyclopamine, and can promote the differentiation of the endocrine precursor cells; FGF10 can promote survival and proliferation of pancreatic endocrine progenitor cells, improve the differentiation efficiency and stability at the stage, and has great synergistic effect on the final pancreatic beta yield; also, the combination of hedgehog pathway antagonist and FGF10 facilitates delivery to PDX1+Differentiation of endocrine precursor cells; the TGF-beta inhibitor is ALK5i II, can promote differentiation and maturation of islet beta cells, and can increase release of insulin in the islet beta cells; the combined use of TGF-beta inhibitors and thyroid hormones may promote NGN3+Amplifying the endocrine precursor cells; the Notch signaling pathway inhibitor is FLI-06 or DAPT, and can increase NGN3+The number of endocrine precursor cells of (a); the C-Met inhibitor is BMS-777607, and can promote the maturation of pancreatic islet beta cells.
The additive A comprises TGF-beta factor, WNT signal pathway activator and JNK signal inhibitor, wherein the concentration of the TGF-beta factor is 30-100ng/mL, preferably 50 ng/mL; the concentration of WNT signaling pathway activator is 1-5. mu.M, preferably 3. mu.M; the concentration of the JNK signal inhibitor is 0.5-5. mu.M, preferably 1. mu.M.
The additive B comprises EGF signal factors, retinoic acid, keratin growth factors, protein kinase C activators and Sirt1 inhibitors, wherein the concentration of the EGF signal factors is 2-20ng/mL, and is preferably 10 ng/mL; retinoic acid (retinoic acid) concentration of 1-5 μ M; the concentration of the keratin growth factor is 20-40ng/mL, preferably 30 ng/mL; the concentration of the protein kinase C activator is 100-300nM, preferably 200 nM; the concentration of the inhibitor of Sirt1 is 10-40. mu.M, preferably 20. mu.M.
The additive B also comprises an ALK4/5/7 inhibitor, and the concentration of the ALK4/5/7 inhibitor is 10-50 nM, preferably 20 nM. The ALK4/5/7 inhibitor is A83-01, can selectively inhibit TGF-beta activin receptors ALK4, I-type receptors ALK5 and node receptors ALK7, can also inhibit the transformation of epithelial cells into mesenchymal cells, and has the functions of promoting the specific differentiation of pancreatic lineage cells and the expansion of pancreatic precursor cells.
The additive C comprises a TGF-beta inhibitor, a hedgehog signaling pathway inhibitor, a Notch signaling pathway inhibitor, an FGF signaling factor and thyroid hormone; wherein the concentration of the TGF-beta inhibitor is 5-20 mu M, preferably 10 mu M; the concentration of the hedgehog signal pathway inhibitor is 10-50ng/mL, preferably 30 ng/mL; the concentration of Notch signaling pathway inhibitor is 0.2-1. mu.M, preferably 0.5. mu.M; the concentration of FGF signal factor is 5-50ng/ml, preferably 10 ng/ml; the concentration of thyroid hormone is 0.1-1. mu.M, preferably 0.5. mu.M.
The additive C also comprises a CDK5 inhibitor, and the concentration of the CDK5 inhibitor is 5-30 nM, preferably 15 nM. The CDK5 inhibitor is AT7519, which has dual efficacy: firstly, activators P35 and P39 of CDK5 are expressed in pancreatic cells and play a role in regulating and controlling the maturation of pancreatic islet beta cells, secondly, AT7519 also has a GSK3 beta inhibitory function and can activate a WNT signal pathway to promote cell differentiation, and in addition, the action of CDK5 inhibitors can be enhanced under the premise of the existence of Notch signal inhibitors.
The additive D comprises a TGF-beta inhibitor, a C-Met inhibitor and trace elements, wherein the concentration of the TGF-beta inhibitor is 5-20 mu M, preferably 10 nM; the concentration of the C-Met inhibitor is 20-100nM, preferably 50 nM.
Additive D also included L-type calcium channel (LTCC) activator and Hippo signaling pathway effector inhibitor; wherein the concentration of the L-type calcium channel (LTCC) activator is 10-30 nM, preferably 20 nM; the concentration of the Hippo signal pathway effector inhibitor is 0.2-0.6. mu.M, preferably 0.35. mu.M. The L-type calcium ion channel (LTCC) activator is BayK8644, can activate Ras signals, enhances the cell cycle and has the function of promoting the proliferation of islet beta cells; the Hippo signal pathway effector YAP inhibitor is Super-TDU1-31, can destroy the interaction between YAP and TEADS transcription factors, and has important effects in promoting the differentiation of endocrine cells and inhibiting the proliferation of precursor cells.
The culture medium of the embodiment adopts Advanced DMEM/F12 culture medium, and is added with some key components such as glucose, sodium bicarbonate, Human Serum Albumin (HSA), glutamine, vitamin C and the like which are necessary for maintaining cell growth, and the added additives comprise agonists or antagonists of signal pathways such as JNK, hedgehog, EGF, FGF, TGF-beta, WNT and the like, and some necessary small molecular compounds, trace elements and the like, and each component can directionally act by stages, and can efficiently induce the directed differentiation of pluripotent stem cells into islet beta cells. By the method, the induced multifunctional stem cells can be directionally differentiated and cultured in a short time to obtain the islet beta cells. Firstly, the quantity of raw materials is not limited, the induced pluripotent stem cells can be expanded infinitely, and moral disputes in the aspect of using the embryonic stem cells can be avoided; secondly, the period for culturing the islet beta cells is about three weeks, compared with the existing differentiation technology, the preparation speed is greatly improved, and the production cost is reduced; thirdly, 3D suspension culture is adopted for culturing the islet beta cells, compared with the existing adherent (2D) culture technology, the yield of differentiated cells is greatly increased, and the loss caused by reagent replacement in the culture process is greatly increased; fourthly, a specific signal pathway inhibitor and a small molecular compound are adopted in the process of culturing the islet beta cells, so that the purity and the yield of the islet beta cells can be greatly improved; fifthly, the cultured islet beta cells selectively adopt a cell sorting or biomaterial wrapping technology, so that poor treatment effect caused by low cell purity or potential safety hazard caused by immunological rejection after cell transplantation can be avoided.
The use method of the additive for inducing the directed differentiation of the pluripotent stem cells to prepare the islet beta cells specifically comprises the following steps:
s100, preparing a culture medium, namely adding an additive A, an additive B, an additive C and an additive D into the same culture medium respectively to obtain a culture medium A, a culture medium B, a culture medium C and a culture medium D;
s200, preparing an induced multifunctional cell ball;
s300, performing primary differentiation, namely adding a culture medium A into the induced multifunctional cell balls, and performing directional differentiation culture to obtain definitive endoderm cells;
s400, performing secondary differentiation, namely adding a culture medium B into the definitive endoderm cells, and performing induced differentiation to obtain pancreatic precursor cells;
s500, performing tertiary differentiation, namely adding a culture medium C into the pancreatic precursor cells, and performing induced differentiation to obtain pancreatic endocrine progenitor cells;
s600, differentiating for four times, adding a culture medium D into the pancreatic endocrine progenitor cells, and inducing and differentiating to obtain the required islet beta cells.
In step S200 of this example, preparing the induced pluripotent stem cell pellet specifically includes digesting the adherent cultured induced pluripotent stem cells into small cell blocks, then resuspending the cells in a complete mTeSR-1 medium containing ROCK1 inhibitor, and resuspending the cells in a complete mTeSR-1 medium of 0.2 × 106/cm2The cell density of the cell is inoculated in an ultra-low adsorption six-hole plate, the cell is cultured for 24 hours under the conditions of 37 ℃ and 5 percent oxygen until the cell becomes a regular pellet, and then 3D suspension culture is carried out for 2-3 days, so as to obtain the induced multifunctional cell pellet.
In this example, step S300 is to add culture medium A to the induced multifunctional cell pellet at 37 deg.C and 5% CO2Culturing for 3 days to obtain definitive endoderm cells, and replacing culture medium A every 1 day during culture.
In this example, step S400 is to add medium B to definitive endoderm cells at 37 ℃ with 5% CO2The pancreatic precursor cells were obtained by further culturing for 5 days under the conditions, and the culture medium B was changed every 1 day during the culture.
Step S500 of this example is embodied in adding medium C to pancreatic precursor cells and adding 5% CO at 37 deg.C2The pancreatic endocrine progenitor cells were obtained by further culturing for 7 days under the conditions, and the medium C was replaced every 1 day during the culture.
In this example, step S600 is to add medium D to pancreatic endocrine progenitor cells at 37 ℃ and 5%CO2Under the condition, the islet beta cells are obtained after 7 days of culture, and the culture medium D is replaced every 2-4 days during the continuous culture period.
Example 2
The content of this example is mainly the preparation of the islet beta cell differentiation medium, which is specifically as follows:
the islet beta cell differentiation medium is divided into four stages, namely a stage I (DE induction medium composed of additive A), a stage II (PP induction medium composed of additive B), a stage III (EN induction medium composed of additive C) and a stage IV (islet beta cell induction medium composed of additive D), wherein the medium components are composed of basic medium components (basal medium) and additive components (supplement), and the components and ratio are as follows 1-4:
TABLE 1 DE Induction Medium composition and proportions
TABLE 2 composition and proportions of PP Induction Medium
Remarking: the addition of A83-01 can obviously improve the cell differentiation efficiency.
TABLE 3 EN Induction Medium composition and proportions
Remarking: the addition of AT7519 can significantly improve the cell differentiation efficiency.
TABLE 4 composition and proportion of islet beta cell induction medium
Remarking: the addition of BayK8644 and Super-TDU1-31 can significantly improve the differentiation efficiency of the cells.
Example 3
The main content of this example is to culture and induce pluripotent stem cells by using a 3D suspension culture method, as follows:
first, required reagent
The culture medium used for inducing the culture of the multifunctional stem cells (iPSCs) is mTeSR-1 complete culture medium, the digestive enzyme used for amplification and passage is EDTA, the reagent used for cell rinsing and balancing is DPBS, and the inhibitor used for promoting the cell survival is Y27632.
Second, culture process
And (3) removing the culture medium from the iPSCs subjected to adherent culture in 100mm-dish, using 2mL of DPBS for moistening, adding 4mL of EDTA digestive enzyme, standing at 37 ℃ for 3-4 min, removing the digestive enzyme after the cells are contacted and separated, adding 12mL of mTeSR-1 complete culture medium containing 10 mu M Y7632 of final concentration, blowing and beating the cells to separate the cells into small cell blocks, wherein 10-30 cells are aggregated in each small cell block.
The separated cell small blocks are averagely transferred to an ultra-low adsorption 6-well plate, cultured for 24h under the condition of 37 ℃ and 5% oxygen until the cells become regular pellets, and then transferred to 75 or 125mL spinner flash for 3D suspension culture at the rotating speed of 70-120 rpm, preferably 100 rpm.
Third, cell ball morphology observation
100mm-dish was placed in a sterile operating table, the suspension cell pellet cultured for 1 or 3 days was transferred to the dish using a 10mL pipette, the dish was gently shaken to uniformly distribute the cell pellet, and observed and photographed under an EVOS microscope.
The results are shown in FIG. 1: the method can obtain large-quantity cell balls with uniform shapes.
Example 4
The main content of this example is directed differentiation culture of islet β cells using induced pluripotent stem cells, as follows:
first, culture of undifferentiated cells
Induction of pluripotent Stem cells 3D suspension cells were obtained as described in example 2, cultured in 125mL of spinner flash, passaged as single cells every 3-4 days using Accutase digestive enzyme, and resuspended in mTeSR-1 complete medium containing 10. mu. M Y7632 inhibitor at 37 ℃ in 5% CO2The incubator is subjected to suspension culture at the rotating speed of 70-100 rpm.
When preparing islet beta cell differentiation, cells digested by Accutase are treated in a 2-8 x 10 manner5cell/mL, preferably 6X 105cell/mL density was plated in spinner flash described above and replaced with differentiation medium after 3 days of culture.
Directed differentiation culture of islet beta cells
Naturally settling the undifferentiated iPSCs cell balls cultured for 3 days for 3-5 min, lightly discarding the upper mTeSR-1 culture medium and dead cells by using a 10mL pipette, sequentially adding the differentiation stage induction culture medium (without marked additives) in example 1 (30mL/Flask, preferably in situ preparation, no more than 7 days if stored at 4 ℃, and taking out 30 minutes before use and balancing to room temperature), standing at 37 ℃, and keeping at 5% CO2The rotating speed is adjusted to 70rpm above the magnetic stirrer in the incubator, and the corresponding stage of culture time is set. Wherein the culture medium is replaced every 2 days, and the culture medium is cultured for 3 days at the first stage, 5 days at the second stage, 7 days at the third stage and more than 7 days at the fourth stage.
Three, pancreatic islet beta cell ball morphology observation
100mm-dish was placed on a sterile operating table, the islet β cell pellet cultured for about three weeks was transferred to dish using a 10mL pipette, and the dish was gently shaken to uniformly distribute the cell pellets, which were then observed under an EVOS microscope.
FIGS. 2A to D show islet beta-cell pellets (including the morphology of the cell pellets at each stage) cultured by the differentiation method of example 4, and it is understood that islet beta-cell pellets having a good morphology can be obtained by this method.
Example 5
The main content of this example is a method for differentiating islet β cells after adding a small molecule compound (to improve differentiation efficiency), which includes the following steps:
using the islet beta cell differentiation method of example 4, small molecule compounds A83-01, AT7519, BayK8644 and Super-TDU1-31 (marked by the symbol x) were selectively added to the induction medium AT the PP, EN and islet beta cell stages of differentiation, and the morphology and number of the resulting islet beta cells were compared after three weeks of culture.
As shown in FIGS. 2-A 'to D', a larger number of islet beta cells could be obtained in the induction medium after addition of the small molecule compound, and the morphology of the islet beta cell spheres was more uniform.
Example 6
The main content of this example is the identification of the expression of a protein characteristic of DE stage cells by immunofluorescence and flow cytometry, as follows:
the method of example 5 was used to perform the directed differentiation of islet beta cells, during which induction of stage one (DE cells) was the initiation and key to differentiation, and the identification of DE cells was performed using immunofluorescence and flow cytometry as follows.
a, immunofluorescence:
placing the cells cultured to the first stage in an ultra-low adsorption 24-pore plate, and standing for 1min to allow the cell balls to sink to the bottom of the pore plate; after carefully aspirating the upper layer of the culture medium, 1 XPBS (pH7.4, the same below, 1 mL/well) was slowly added along the wall of the 24-well plate and washed 2 times; 4% PFA (paraformaldehyde, 0.5 mL/well) pre-warmed at 37 ℃ was then added slowly along the plate walls, the cells were fixed for 18 minutes at room temperature, the PFA was gently aspirated, and the plates were washed 3 times (1 mL/well/time) with 1 XPBS.
0.3% Triton X100 (0.5 mL/well) was added and incubated at 37 ℃ for 30 minutes; then blocked by adding 5% BSA (0.5 mL/well) and incubated at 37 ℃ for 30 min; primary antibody (SOX17 and FOXA2) was then added directly to the blocking solution in an amount of 10. mu.L/well, incubated at 37 ℃ for 1 hour, and washed 3 times with 1 XPBS (1 mL/well/time).
Adding a secondary antibody diluted by 1% BSA (dilution ratio 1:1000), wherein the addition amount of the secondary antibody is 0.5 mL/hole, incubating at 37 ℃ for 60 minutes in the dark, absorbing the secondary antibody, adding 1 XPBS, and washing for 3 times (1 mL/hole/time) for 5 minutes each time; then, 1. mu.g/mL DAPI (0.5 mL/well) prepared with 1 XPBS was added to the cells, stained for 5 minutes, the DAPI was aspirated, and the cells were washed 2 times with 1 XPBS (1 mL/well/time); finally, 1 × PBS (0.5 mL/well) was added to resuspend the cell pellet, and the pellet was observed under a microscope and photographed for processing.
Remarking: in addition to immunofluorescent staining of the cell spheres as per the protocol described above, the spheres were also plated for immunofluorescence in 24-well plates using Accutase digestion into single cells (better to see the expression of each marker in the single cells).
b, flow cytometry:
placing the cell balls finished in the first stage of culture in a 15mL centrifuge tube, naturally settling for 1-3 min, then discarding the culture solution, and rinsing once by using 1mL DPBS and discarding; adding 1-2 mL of Accutase or TrypLE digestive juice into the cell balls, placing the cell balls at 37 ℃ for 3-5 minutes, and separating the cell balls into single cells; then adding 2-3 times of DPBS to dilute the digestive juice, centrifuging at a rotating speed of 200-300 g for 3 minutes, and discarding the digestive juice and the DPBS.
1mL of 4% Paraformaldehyde (PFA) was added to the cells, fixed at 4 ℃ for 30 minutes and discarded; then adding 0.3% Triton X100 (1mL) or other stabilizing buffer, sealing and permeating for 30 minutes at 4 ℃, and then discarding; primary antibody (SOX17 and FOXA2) in 1mL blocking solution was then added overnight at 4 ℃.
The primary antibody is discarded the next day, 1mL of secondary antibody prepared by confining liquid is added, and the mixture is incubated for 2 hours at 4 ℃ in a dark place; finally, flow cytometry analysis is carried out.
FIGS. 3-A-B 'and FIGS. 3-C' are immunofluorescence and flow cytometric assay results, respectively, of the DE cells cultured in example 5, and it is known that the cultured DE cells all have high marker molecule expression.
Example 7
The main content of this example is the identification of the expression of a protein characteristic to cells in the PP stage by immunofluorescence, as follows:
cells (PP cells) cultured in stage two were removed and the expression of the key proteins (PDX1 and NKX6.1) in the PP stage cells was examined according to the immunofluorescence procedure of example 6.
FIG. 4 shows the results of immunofluorescence assay of Marker markers (PDX1 and NKX6.1) of PP cells cultured in example 5, which indicates that the PP cells cultured have high Marker molecule expression.
Example 8
The main content of this example is the identification of the expression of a protein characteristic to pancreatic islet β cells using immunofluorescence, as follows:
the cells (islet β cells) cultured to stage four were removed, and the expression of islet β cell key proteins (NKX6.1, C-peptide, insulin) was detected according to the immunofluorescence procedure of example 6.
FIG. 5-A shows the immunofluorescence assay results of islet β cells cultured in example 5, indicating that islet β cells cultured have high expression of marker molecules (NKX6.1, C-peptide, insulin, and MAFA).
Example 9
The main content of this example is to use an Elisa kit to detect the insulin secretion function of pancreatic islet β cells, which includes the following details:
collecting the islet beta cell spheres (20-30) cultured for 7 days in the fourth stage in a 15mL centrifuge tube by using a 5mL pipette, naturally settling the cell spheres for 1-3 minutes, and then removing the upper-layer culture solution. Then, 1mL of 70% ethanol containing 1.5% hydrochloric acid (HCl) was added thereto, and the mixture was left at-20 ℃ for 24 hours. After 24 hours the cell pellet was gently shaken and allowed to stand for another 24 hours.
After 48 hours of standing, the tube was placed in a centrifuge and centrifuged at 2100rcf for 15 minutes, and 1mL of the supernatant was collected in a new 15mL centrifuge tube and neutralized with 1mL of 1M TRIS (pH 7.5). The neutralized liquid was tested for insulin release using the human insulin Elisa kit.
FIG. 5-B shows the results of measurement of insulin release from islet β cells cultured in example 5, which indicates that islet β cells cultured in example 5 have a high insulin release amount.
Example 10
The main content of this example is to use Elisa kit to detect the GSIS function of pancreatic islet β cells, which is as follows:
preparation of reagents: krb buffer was made up of 128mM NaCl, 5mM KCl, 2.7mM CaCl2, 1.2mM MgCl2, 1mM Na2HPO4, 1.2mM KH2PO4, 5mM NaHCO3, 10mM HEPES, and 0.1% BSA with deionized water; low concentration glucose (2mM) and high concentration glucose (20mM) were formulated from Krb buffer; the 30mM KCl is prepared from a 1M KCl aqueous solution and a 20mM high-concentration glucose solution; all reagents were filter sterilized through 0.22 μ M filter after formulation.
GSIS functional verification: firstly, islet beta cell spheres (20) cultured for 14 days in stage four are collected in a 15mL centrifuge tube by using a 5mL pipette, and the cell spheres naturally settle for 1-3 minutes and then the supernatant culture solution is discarded. Then, 1mL of Krb buffer was added for rinsing and pre-incubation with 200. mu.L of low concentration (2mM) glucose Krb solution for 1 hour was used to remove residual insulin. After rinsing again with 1mL of Krb buffer 2 times, the cell pellet was incubated with 200. mu.L of low concentration (2mM), high concentration (2mM) glucose Krb solution and KCl solution for 1 hour (each time the solution was changed to a different concentration, the cell pellet was rinsed with Krb equilibration solution, and the supernatant was collected at the end of the incubation). Finally, 200. mu.L of glucose and KCl stimulated supernatant were collected and insulin Elisa kit was used to detect insulin release (GSIS) levels of islet beta cells stimulated with different concentrations of glucose.
FIG. 5-C shows the GSIS measurements of islet beta cells cultured in example 4, indicating that islet beta cells cultured in example 4 have different levels of insulin release in response to different concentrations of glucose stimulation, and that the amount of insulin released from islet beta cells stimulated to increase with increasing glucose content is highest after KCl stimulation.
From the identification results of examples 4-10, it can be seen that the cells obtained by the induced pluripotent stem cell directed differentiation culture using the additive and the use method of the present invention not only have the expression of proteins characteristic to pancreatic islet β cells, such as PDX1, NKX6.1, C-Peptide and Insulin, but also have high yield and purity, and have the glucose stimulation-Insulin release (GSIS) function, indicating that the cells are mature and functional pancreatic islet β cells.
Compared with other differentiation methods, the additive of the invention ensures that the induced multifunctional stem cells cultured by 3D suspension are directionally differentiated into the islet beta cells, and has the following advantages: firstly, the quantity of raw materials is not limited, and induced pluripotent stem cells can be expanded infinitely; secondly, the raw material source is not limited, the induced multifunctional stem cells can be obtained by reprogramming adult cells of healthy people such as peripheral blood mononuclear cells, and the use of the embryonic stem cells has ethical limitation; thirdly, the differentiation efficiency is high, and mature and functional islet beta cells can be obtained by using suspension culture and specific signal pathway protein factors and small molecular compounds; fourthly, the obtained yield is high, and mature islet beta cells with large quantity and higher purity can be obtained by adding a specific small molecular compound; fifthly, the time consumption is short, the culture period is 22-30 days, higher cell yield can be obtained by prolonging the amplification time, and the cost loss is reduced; and sixthly, the method is safe and reliable, and the purity of the obtained cells can be improved or the immune rejection of the cells to a host can be reduced or even eliminated by using methods such as cell sorting or biological material wrapping.
In addition, the use of small molecule compounds such as ALK4/5/7 inhibitors (A83-01), CDK5 inhibitors (AT7519), LTCC activators (BayK8644) and Hippo signaling pathway effector inhibitors (Super-TDU1-31) promotes the directed differentiation of islet beta cells, as evidenced by the massive expansion of intermediates (pancreatic precursor cells) during differentiation, and the maturation and high expansion efficiency of the differentiation products (islet beta cells). The islet beta cells obtained by the additive and the using method have the advantages of high quantity, high maturity and complete functions, and a new method is provided for the treatment of future diabetes.
The above-mentioned embodiments only express a certain implementation mode of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the present invention; it should be noted that, for those skilled in the art, without departing from the concept of the present invention, several variations and modifications can be made, which are within the protection scope of the present invention; therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (16)
1. An additive for inducing pluripotent stem cells to directionally differentiate and prepare islet beta cells is characterized in that: the additive comprises a JNK inhibitor, a hedgehog pathway antagonist, an EGF signal factor, an FGF signal factor, a TGF-beta inhibitor, a WNT signal pathway activator, a Notch signal pathway inhibitor, a small molecular compound and trace elements, and the proportion of the additive in different stages of cell culture is different.
2. The additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 1, wherein: the JNK inhibitor is CC-930, the hedgehog pathway antagonist is N-acetylcysteine or cyclopamine, the EGF signal factor is EGF, the FGF signal factor is FGF10, the TGF-beta superfamily factor is ActivinA, the TGF-beta inhibitor is ALK5iII, the WNT signal pathway activator is BML-284 or WNT3a, and the Notch signal pathway inhibitor is FLI-06 or DAPT.
3. The additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 1, wherein: the small molecule compounds include keratin growth factor, protein kinase C activator, ROCK1 inhibitor, Sirt1 inhibitor, C-Met inhibitor, thyroid hormone and retinoic acid (Retinoic acid).
4. The additive for inducing pluripotent stem cells to differentiate directionally into islet beta cells according to claim 3, wherein the additive comprises: the keratin growth factor is KGF, the protein kinase C activator is TPB, the ROCK1 inhibitor is Y27632, the Sirt1 inhibitor is Nicotinamide, the C-Met inhibitor is BMS-777607, and the thyroid hormone is T3.
5. The additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 1, wherein: also comprises an ALK4/5/7 inhibitor, a CDK5 inhibitor, an L-type calcium channel (LTCC) activator and a Hippo signal channel effector inhibitor, wherein the ALK4/5/7 inhibitor is A83-01, the CDK5 inhibitor is AT7519, the L-type calcium channel (LTCC) activator is BayK8644, and the Hippo signal channel effector inhibitor is Super-TDU 1-31.
6. The additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 1, wherein: the additive has different proportions in different stages of cell culture, and can be specifically divided into an additive A, an additive B, an additive C and an additive D, wherein the additive A is used for inducing the differentiation of the multifunctional cell balls into the stage of definitive endoderm, the additive B is used for inducing the differentiation of the definitive endoderm into the stage of pancreatic precursor cells, the additive C is used for inducing the differentiation of the pancreatic precursor cells into pancreatic endocrine progenitor cells, and the additive D is used for inducing the differentiation of the pancreatic endocrine progenitor cells into pancreatic islet beta cells.
7. The additive for inducing pluripotent stem cells to differentiate directionally into islet beta cells according to claim 6, wherein the additive comprises: the additive A comprises a TGF-beta factor, a WNT signal pathway activator and a JNK signal inhibitor, wherein the concentration of the TGF-beta factor is 30-100 ng/mL; the concentration of WNT signal pathway activator is 1-5 μ M; the concentration of JNK signal inhibitor is 0.5-5 μ M.
8. The additive for inducing pluripotent stem cells to differentiate directionally into islet beta cells according to claim 6, wherein the additive comprises: the additive B comprises EGF signal factors, retinoic acid, keratin growth factors, protein kinase C activators and Sirt1 inhibitors, wherein the concentration of the EGF signal factors is 2-20 ng/mL; retinoic acid (retinoic acid) concentration of 1-5 μ M; the concentration of the keratin growth factor is 20-40 ng/mL; the concentration of the protein kinase C activator is 100-300 nM; the concentration of Sirt1 inhibitor was 10-40. mu.M.
9. The additive for inducing pluripotent stem cells to differentiate directionally into islet beta cells according to claim 6, wherein the additive comprises: the additive C comprises a TGF-beta inhibitor, a hedgehog signaling pathway inhibitor, a Notch signaling pathway inhibitor, an FGF signaling factor and thyroid hormone; wherein the concentration of the TGF-beta inhibitor is 5-20 mu M; the concentration of the hedgehog signal pathway inhibitor is 10-50 ng/mL; the concentration of Notch signaling pathway inhibitor is 0.2-1 μ M; the concentration of FGF signal factor is 5-50 ng/ml; the concentration of thyroid hormone is 0.1-1 μ M.
10. The additive for inducing pluripotent stem cells to differentiate directionally into islet beta cells according to claim 6, wherein the additive comprises: the additive D comprises a TGF-beta inhibitor, a C-Met inhibitor and trace elements, wherein the concentration of the TGF-beta inhibitor is 5-20 mu M; the concentration of the C-Met inhibitor is 20-100 nM.
11. Use of an additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells, comprising the steps of:
s100, preparing a culture medium, namely adding an additive A, an additive B, an additive C and an additive D into the same culture medium respectively to obtain a culture medium A, a culture medium B, a culture medium C and a culture medium D;
s200, preparing an induced multifunctional cell ball;
s300, performing primary differentiation, namely adding a culture medium A into the induced multifunctional cell balls, and performing directional differentiation culture to obtain definitive endoderm cells;
s400, performing secondary differentiation, namely adding a culture medium B into the definitive endoderm cells, and performing induced differentiation to obtain pancreatic precursor cells;
s500, performing tertiary differentiation, namely adding a culture medium C into the pancreatic precursor cells, and performing induced differentiation to obtain pancreatic endocrine progenitor cells;
s600, differentiating for four times, adding a culture medium D into the pancreatic endocrine progenitor cells, and inducing and differentiating to obtain the required islet beta cells.
12. The use method of the additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 11, wherein the additive comprises: the step S200 of preparing the induced multifunctional cell pellet is to digest the adherent culture induced multifunctional stem cells into small cell blocks, then use mTeSR-1 complete culture medium containing ROCK1 inhibitor for heavy suspension, and use the mTeSR-1 complete culture medium for 0.2 multiplied by 106/cm2The cell density of the cell is inoculated in an ultra-low adsorption six-hole plate, the cell is cultured for 24 hours under the conditions of 37 ℃ and 5 percent oxygen until the cell becomes a regular pellet, and then 3D suspension culture is carried out for 2-3 days, so as to obtain the induced multifunctional cell pellet.
13. The use method of the additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 11, wherein the additive comprises: the step S300 is to add the culture medium A into the induced multifunctional cell balls, and to add 5% CO at 37 DEG C2Culturing for 3 days to obtain definitive endoderm cells, and replacing culture medium A every 1 day during culture.
14. The use method of the additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 11, wherein the additive comprises: the step S400 is to add the culture medium B to the definitive endoderm cells, and to add 5% CO at 37 ℃2The pancreatic precursor cells were obtained by further culturing for 5 days under the conditions, and the culture medium B was changed every 1 day during the culture.
15. The use method of the additive for inducing the directed differentiation of pluripotent stem cells to produce islet beta cells according to claim 11, wherein the additive comprises: the step S500 is to add the culture medium A into the pancreatic precursor cells, and to add 5% CO at 37 deg.C2The pancreatic endocrine progenitor cells were obtained by further culturing for 7 days under the conditions, and the medium C was replaced every 1 day during the culture.
16. The method for inducing pluripotent stem cells to differentiate directionally into pancreas according to claim 11The use method of the additive for the island beta cells is characterized in that: the step S600 is to add the culture medium A to the pancreatic endocrine progenitor cells and 5% CO at 37 deg.C2Under the condition, the islet beta cells are obtained after 7 days of culture, and the culture medium D is replaced every 2-4 days during the continuous culture period.
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