SG10201707485YA - Methods and devices for analysis of defined multicellular combinations - Google Patents

Methods and devices for analysis of defined multicellular combinations

Info

Publication number
SG10201707485YA
SG10201707485YA SG10201707485YA SG10201707485YA SG10201707485YA SG 10201707485Y A SG10201707485Y A SG 10201707485YA SG 10201707485Y A SG10201707485Y A SG 10201707485YA SG 10201707485Y A SG10201707485Y A SG 10201707485YA SG 10201707485Y A SG10201707485Y A SG 10201707485YA
Authority
SG
Singapore
Prior art keywords
combinations
analysis
methods
devices
multicellular
Prior art date
Application number
SG10201707485YA
Other languages
English (en)
Inventor
Jason A A West
Brian Fowler
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of SG10201707485YA publication Critical patent/SG10201707485YA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5097—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving plant cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/10—Means to control humidity and/or other gases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201707485YA 2013-03-15 2014-03-14 Methods and devices for analysis of defined multicellular combinations SG10201707485YA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361852135P 2013-03-15 2013-03-15

Publications (1)

Publication Number Publication Date
SG10201707485YA true SG10201707485YA (en) 2017-10-30

Family

ID=51538375

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201707485YA SG10201707485YA (en) 2013-03-15 2014-03-14 Methods and devices for analysis of defined multicellular combinations
SG11201504876TA SG11201504876TA (en) 2013-03-15 2014-03-14 Methods and devices for analysis of defined multicellular combinations

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201504876TA SG11201504876TA (en) 2013-03-15 2014-03-14 Methods and devices for analysis of defined multicellular combinations

Country Status (7)

Country Link
US (2) US10578633B2 (zh)
EP (2) EP2970849B1 (zh)
JP (1) JP2016521350A (zh)
CN (1) CN105164246B (zh)
CA (1) CA2895638A1 (zh)
SG (2) SG10201707485YA (zh)
WO (1) WO2014144789A2 (zh)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
SG11201609055RA (en) 2014-05-08 2016-11-29 Fluidigm Corp Integrated single cell sequencing
CA2965273C (en) 2014-10-27 2021-08-17 Universidade De Aveiro Equipment for chromatic discrimination and counting of organisms
US9996920B2 (en) 2014-12-09 2018-06-12 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
US10751715B1 (en) * 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
WO2017040548A1 (en) 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
US20170160187A1 (en) * 2015-12-08 2017-06-08 Tdk Corporation Laser Scanner Particle Counter and Imager
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
EP3514518A4 (en) * 2016-09-16 2020-09-02 On-chip Biotechnologies Co., Ltd. FINE PARTICLE DISTRIBUTION DEVICE, FINE PARTICLE ANALYSIS DEVICE, REACTION DETECTION DEVICE AND PROCESS USING SAID DEVICES
KR102300480B1 (ko) 2016-11-17 2021-09-13 콤비네티 인코포레이티드 핵산 분석 및 정량화를 위한 방법 및 시스템
AU2017366833B2 (en) * 2016-12-02 2020-02-20 EMULATE, Inc. In vitro epithelial models comprising lamina propria-derived cells
US11579073B2 (en) * 2017-02-07 2023-02-14 Nodexus Inc. Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof
KR101897250B1 (ko) * 2017-07-11 2018-09-10 광주과학기술원 항암제 조합에 대한 단일 세포 분석 칩
US10391493B2 (en) * 2017-08-29 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
TWI699494B (zh) 2018-01-24 2020-07-21 美商伊路米納有限公司 流體緩衝
GB2585148B (en) * 2018-02-07 2023-03-29 Emulate Inc Gas mixer and pressurizer apparatus
EP3752828A4 (en) * 2018-02-16 2021-11-24 Astrego Diagnostics AB MICROFLUIDIC DEVICE
US11278888B2 (en) 2018-04-03 2022-03-22 Hewlett-Packard Development Company, L.P. Microfluidic channels to convey cells of different sizes
WO2019243598A1 (en) * 2018-06-21 2019-12-26 Astraveus Microfluidic device and method for processing particles
US20210364410A1 (en) * 2018-12-21 2021-11-25 Sony Corporation Particle confirming method, particle trapping chip, and particle analyzing system
CN114556258A (zh) * 2019-04-24 2022-05-27 班努生物科技公司 用于分选和富集感兴趣的靶细胞的定位辅助的阴性颗粒排斥（panr）
WO2021110896A1 (en) * 2019-12-04 2021-06-10 Astraveus Microfluidic device and method for processing particles
US20230191402A1 (en) * 2020-05-18 2023-06-22 National University Of Singapore Microfluidic device and liquid control system therefor
EP4175568A1 (en) * 2020-07-06 2023-05-10 Cornell University Methods and devices for automated microfluidic oocyte denudation
US11498067B2 (en) * 2020-10-23 2022-11-15 City University Of Hong Kong Apparatus and a method for patterning biological cells

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4119955C2 (de) 1991-06-18 2000-05-31 Danfoss As Miniatur-Betätigungselement
US5417235A (en) 1993-07-28 1995-05-23 Regents Of The University Of Michigan Integrated microvalve structures with monolithic microflow controller
US6267858B1 (en) 1996-06-28 2001-07-31 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
US5785295A (en) 1996-08-27 1998-07-28 Industrial Technology Research Institute Thermally buckling control microvalve
EP0972082A4 (en) 1997-04-04 2007-04-25 Caliper Life Sciences Inc BIOCHEMICAL ANALYZERS OPERATING IN CLOSED LOOP
DE60031540T2 (de) 1999-06-28 2007-05-16 California Institute Of Technology, Pasadena Mikromechanische pump- und ventilsysteme
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US7258774B2 (en) 2000-10-03 2007-08-21 California Institute Of Technology Microfluidic devices and methods of use
WO2002065005A1 (en) 2000-11-06 2002-08-22 California Institute Of Technology Electrostatic valves for microfluidic devices
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US20020164816A1 (en) 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US7075162B2 (en) 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
WO2003085379A2 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
CA2499245A1 (en) 2002-09-16 2004-03-25 Cytonome, Inc. Method and apparatus for sorting particles
CA2500283A1 (en) 2002-09-25 2004-04-08 California Institute Of Technology Microfluidic large scale integration
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
AU2003297214A1 (en) * 2002-12-16 2004-07-22 Cytodiscovery, Inc. Microfluidic system with integrated permeable membrane
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
ES2383689T3 (es) 2003-09-23 2012-06-25 University Of North Carolina At Chapel Hill Perfluoropoliéteres fotocurables para su uso como materiales novedosos en dispositivos microfluídicos
US20070275193A1 (en) 2004-02-13 2007-11-29 Desimone Joseph M Functional Materials and Novel Methods for the Fabrication of Microfluidic Devices
CN103884698B (zh) 2004-06-07 2017-04-12 先锋生物科技股份有限公司 用于微流体器件的光学透镜***和方法
US20080264863A1 (en) 2004-12-03 2008-10-30 California Institute Of Technology Microfluidic Sieve Valves
EP1915618A4 (en) * 2005-06-02 2009-09-30 Fluidigm Corp ANALYSIS USING MICROFLUIDIC SEPARATION DEVICES
US9376658B2 (en) * 2008-01-03 2016-06-28 Emd Millipore Corporation Cell culture array system for automated assays and methods of operation and manufacture thereof
SG173048A1 (en) 2009-01-20 2011-08-29 Univ Leland Stanford Junior Single cell gene expression for diagnosis, prognosis and identification of drug targets
US20120100538A1 (en) 2009-03-24 2012-04-26 Biocept, Inc. Devices and methods of cell capture and analysis
JP5923035B2 (ja) 2009-03-24 2016-05-24 バイオセプト インコーポレイティッド 細胞の捕捉および解析のデバイスおよび方法
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
WO2012106668A2 (en) 2011-02-03 2012-08-09 Fluidigm Corporation Multifunctional probe-primers
WO2012154876A1 (en) 2011-05-09 2012-11-15 Fluidigm Corporation Probe based nucleic acid detection
WO2012162779A1 (en) 2011-05-27 2012-12-06 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
WO2012178166A1 (en) * 2011-06-24 2012-12-27 Arryx, Inc. Method and apparatus for fractionating genetically distinct cells and cellular components
US9952126B2 (en) 2012-02-29 2018-04-24 Fluidigm Corporation Methods for multiple single-cell capturing and processing using micro fluidics

Also Published As

Publication number Publication date
EP2970849B1 (en) 2019-08-21
US20200264205A1 (en) 2020-08-20
EP3581641A1 (en) 2019-12-18
CN105164246A (zh) 2015-12-16
US20160025761A1 (en) 2016-01-28
EP2970849A4 (en) 2016-11-02
JP2016521350A (ja) 2016-07-21
WO2014144789A3 (en) 2014-12-24
US10578633B2 (en) 2020-03-03
CN105164246B (zh) 2018-09-25
EP2970849A2 (en) 2016-01-20
WO2014144789A2 (en) 2014-09-18
CA2895638A1 (en) 2014-09-18
SG11201504876TA (en) 2015-07-30

Similar Documents

Publication Publication Date Title
SG11201504876TA (en) Methods and devices for analysis of defined multicellular combinations
IL252544A0 (en) Devices and methods for analyzing mouse behavior
GB2547875B (en) Methods for meta-genomics analysis of microbes
GB201309568D0 (en) Methods and apparatus
EP2953041A4 (en) METHOD AND DEVICE FOR ANALYZING FORM OPTIMIZATION
EP2953040A4 (en) METHOD AND DEVICE FOR ANALYZING FORM OPTIMIZATION
PL3056056T3 (pl) Sposób i układ wykrywania urządzeń
GB2513304B (en) Peripheral apparatus and method of construction
PL3087198T3 (pl) Metagenomowa analiza próbek
PL3071969T3 (pl) Sposób analizy
EP2946614A4 (en) METHOD AND APPARATUS
ZA201600927B (en) Structural assembly and method of assembly thereof
EP2963410A4 (en) PARTICLE ANALYSIS DEVICE
GB201604534D0 (en) Nucleic-acid-sequence determination device and nucleic-acid-sequence determination method
ZA201602719B (en) Hydraulic devices and methods of actuating same
GB201319706D0 (en) Real time analysis of big data
GB201301728D0 (en) Telcommunications apparatus and methods
GB201421886D0 (en) Metagenomic analysis of samples
EP2972190A4 (en) DEVICE AND METHOD OF ANALYSIS
EP2971145A4 (en) VISIBLE BREATHING SYSTEM AND METHOD
GB201307787D0 (en) Methods and Apparatus
EP2966433A4 (en) DEVICE FOR SETTING AN INTEREST RANGE FOR ANALYSIS
TWM475581U (en) Analysis system of behavior
GB2519734B (en) Apparatus and Methods
GB201316737D0 (en) Apparatus and Methods