SE8605095D0 - METHOD OF DETECTING VEXVIRUS - Google Patents
METHOD OF DETECTING VEXVIRUSInfo
- Publication number
- SE8605095D0 SE8605095D0 SE8605095A SE8605095A SE8605095D0 SE 8605095 D0 SE8605095 D0 SE 8605095D0 SE 8605095 A SE8605095 A SE 8605095A SE 8605095 A SE8605095 A SE 8605095A SE 8605095 D0 SE8605095 D0 SE 8605095D0
- Authority
- SE
- Sweden
- Prior art keywords
- virus
- biotin
- rna
- detecting
- plant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A process for detecting plant virus, in which plant sap is treated with a protein-decomposing enzyme for removal of endogenously bound biotin, and in which virus RNA from the plant sap is bound to a filter membrane and prehybridisation is carried out, whereupon virus RNA is hybridised by incubation of the membrane with biotin-labelled DNA which is homologous to RNA in the virus to be detected, the presence of the specific virus RNA becoming visually detectable through the occurrence of a colour after addition of a biotin-specific enzyme.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8605095A SE455600B (en) | 1986-11-27 | 1986-11-27 | PROCEDURE FOR DETECTING VEXVIRUS AND ITS APPLICATION FOR DETECTING ROOTSOTVIRUS AND POTATISVIRUS |
AU83382/87A AU8338287A (en) | 1986-11-27 | 1987-11-25 | Process for detecting plant virus |
PCT/SE1987/000558 WO1988003956A1 (en) | 1986-11-27 | 1987-11-25 | Process for detecting plant virus |
DK382488A DK382488D0 (en) | 1986-11-27 | 1988-07-08 | METHOD OF DETECTING PLANT VIRUS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8605095A SE455600B (en) | 1986-11-27 | 1986-11-27 | PROCEDURE FOR DETECTING VEXVIRUS AND ITS APPLICATION FOR DETECTING ROOTSOTVIRUS AND POTATISVIRUS |
Publications (3)
Publication Number | Publication Date |
---|---|
SE8605095D0 true SE8605095D0 (en) | 1986-11-27 |
SE8605095L SE8605095L (en) | 1988-05-28 |
SE455600B SE455600B (en) | 1988-07-25 |
Family
ID=20366432
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8605095A SE455600B (en) | 1986-11-27 | 1986-11-27 | PROCEDURE FOR DETECTING VEXVIRUS AND ITS APPLICATION FOR DETECTING ROOTSOTVIRUS AND POTATISVIRUS |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU8338287A (en) |
SE (1) | SE455600B (en) |
WO (1) | WO1988003956A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8827094D0 (en) * | 1988-11-19 | 1988-12-21 | Scottish Crop Research Inst | Dna sequence encoding coat protein gene of potato leafroll virus |
IL97295A0 (en) * | 1990-02-27 | 1992-05-25 | Agrilab Biotechnology Ltd | Procedure for the detection of plant pathogens performed under field conditions and a diagnostic kit for its application |
CN1303423C (en) * | 2004-04-15 | 2007-03-07 | 云南省农业科学院 | PVY Yunnan isolate TAS-ELISA test kit and its preparing method |
CN1303422C (en) * | 2004-04-15 | 2007-03-07 | 云南省农业科学院 | PVX Yunnan isolate TAS-ELISA test kit and its preparing method |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338856C (en) * | 1983-07-05 | 1997-01-21 | Jannis Stavrianopoulos | In vivo labelling of polynucleotide sequences |
GB8331071D0 (en) * | 1983-11-22 | 1983-12-29 | Karayiannis P | Assay for dna/rna |
JPH06100605B2 (en) * | 1984-04-05 | 1994-12-12 | ハワ−ド フロ−リ インステイチユ−ト オブ イクスペリメンタル フイジオロジ アンド メデイスン | Hybridization histochemistry |
EP0189280A3 (en) * | 1985-01-23 | 1987-01-07 | Dekalb-Pfizer Genetics | Qualitative testing for nucleic acid |
-
1986
- 1986-11-27 SE SE8605095A patent/SE455600B/en not_active IP Right Cessation
-
1987
- 1987-11-25 WO PCT/SE1987/000558 patent/WO1988003956A1/en not_active Application Discontinuation
- 1987-11-25 AU AU83382/87A patent/AU8338287A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1988003956A1 (en) | 1988-06-02 |
SE8605095L (en) | 1988-05-28 |
AU8338287A (en) | 1988-06-16 |
SE455600B (en) | 1988-07-25 |
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Legal Events
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---|---|---|---|
NUG | Patent has lapsed |
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