RU2002114044A - L-пантолактон-гидролаза и способ получения d-пантолактона - Google Patents
L-пантолактон-гидролаза и способ получения d-пантолактона Download PDFInfo
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- RU2002114044A RU2002114044A RU2002114044/13A RU2002114044A RU2002114044A RU 2002114044 A RU2002114044 A RU 2002114044A RU 2002114044/13 A RU2002114044/13 A RU 2002114044/13A RU 2002114044 A RU2002114044 A RU 2002114044A RU 2002114044 A RU2002114044 A RU 2002114044A
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- Prior art keywords
- acid sequence
- pantolactone
- nucleic acid
- microorganism
- hydrolase
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
- C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
- C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (20)
1. Выделенная последовательность нуклеиновых кислот, кодирующая полипептид с активностью L-пантолактон-гидролазы, выбранная из группы, включающей а) последовательность нуклеиновых кислот с представленной в SEQ ID NO: 1 последовательностью, б) последовательности нуклеиновых кислот, которые отводятся как результат дегенерированного генетического кода от представленной в SEQ ID NO: 1 последовательности нуклеиновых кислот, в) производные представленной в SEQ ID NO: 1 последовательности нуклеиновых кислот, которые кодирует полипептиды с представленной в SEQ ID NO: 2 аминокислотной последовательностью и которые имеют по меньшей мере 50% гомологии на аминокислотном уровне, без значительного снижения ферментативной активности полипептидов,
г) функциональные эквиваленты приведенных в пунктах от (а) до (в) последовательностей.
2. Аминокислотная последовательность, кодированная последовательностью нуклеиновых кислот по п.1.
3. Аминокислотная последовательность по п.2, кодированная представленной в SEQ ID NO: 1 последовательностью.
4. Конструкт нуклеиновой кислоты, содержащий последовательность нуклеиновых кислот по п.1, причем последовательность нуклеиновых кислот связана с несколькими регуляторными сигналами.
5. Вектор, содержащий последовательность нуклеиновых кислот по п.1 или конструкт нуклеиновых кислот по п.4.
6. Микроорганизм, содержащий последовательность нуклеиновых кислот по п.1, по меньшей мере один конструкт нуклеиновых кислот по п.4 или вектор по п.5.
7. Микроорганизм по п.6, отличающийся тем, что микроорганизмом является грам-отрицательной бактерией.
8. Микроорганизм по п.6 или 7, отличающийся тем, что микроорганизмом является бактерия из группы, включающей α-протеобактерии, β-протеобактерии или γ-протеобактерии.
9. Микроорганизм по пп.6-8, отличающийся тем, что микроорганизмом является бактерия из семейства Enterobacteriaceae, Pseudomonadaceae или Rhizobiaceae.
10. Микроорганизм по пп.6-8, отличающийся тем, что микроорганизмом является бактерия вида Agrobacterium, Pseudomonas, Burkholderia, Salmonella или Escherichia.
11. L-пантолактон-гидролаза, отличающаяся следующими свойствами: a) превращением L-пантолактона в соответствующую кислоту, b) стабильностью значения рН: L-пантолактон гидролаза стабильна в диапазоне значений рН от 4 до 10, c) оптимальными значениями рН: от 7,2 до 7,6, d) оптимальными температурами 70-75°С, e) не имеет места ингибирование активности посредством этилендиамино-тетрауксусной кислоты.
12. Способ получения D-пантолактона, отличающийся тем, что он включает следующие стадии: а) взаимодействие рацемического пантолактона в присутствии L-пантолактон-гидролазы по п.11 или L-пантолактон-гидролазы с аминокислотной последовательности по п.2 или микроорганизма по п.6 с получением D-пантолактона и L-пантоиновой кислоты, б) отделение D-пантолактона.
13. Способ по п.12, отличающийся тем, что полученную на стадии (б) L-пантоиновую кислоту рацемизируют и возвращают на стадию (а).
14. Способ по п.12 или 13, отличающийся тем, что взаимодействие рацемического пантолактона проводят в присутствии иммобилизированной L-пантолактон-гидролазы по п.11 или иммобилизированной L-пантолактон-гидролазы с аминокислотной последовательностью по п.2.
15. Способ по п.12 или 13, отличающийся тем, что взаимодействие рацемического пантолактона проводят в присутствии промытого, покоящегося или вскрытого микроорганизма по п.6.
16. Способ по п.12 или 15, отличающийся тем, что микроорганизм иммобилизирован.
17. Способ по пп.12-16, отличающийся тем, что его проводят в водной реакционной смеси при значении рН между 4 и 12.
18. Способ по пп.12-17, отличающийся тем, что его проводят при температуре 0-95°С.
19. Способ по п.12 или 13, отличающийся тем, что D-пантолактон отделяют экстракцией.
20. Способ по пп.12-18, отличающийся тем, что D-пантолактон имеет оптическую чистоту по меньшей мере 90%.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19952501.3 | 1999-10-29 | ||
DE19952501A DE19952501A1 (de) | 1999-10-29 | 1999-10-29 | L-Pantolacton-Hydrolase und ein Verfahren zur Herstellung von D-Pantolacton |
DE10029194.5 | 2000-06-19 | ||
DE10029194A DE10029194A1 (de) | 2000-06-19 | 2000-06-19 | L-Pantolacton-Hydrolase und ein Verfahren zur Herstellung von D-Pantolacton |
Publications (1)
Publication Number | Publication Date |
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RU2002114044A true RU2002114044A (ru) | 2004-03-27 |
Family
ID=26006072
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2002114044/13A RU2002114044A (ru) | 1999-10-29 | 2000-10-20 | L-пантолактон-гидролаза и способ получения d-пантолактона |
Country Status (17)
Country | Link |
---|---|
US (1) | US6998258B1 (ru) |
EP (1) | EP1224296A1 (ru) |
JP (1) | JP2003530080A (ru) |
KR (1) | KR20020043254A (ru) |
CN (1) | CN1384880A (ru) |
AU (1) | AU782517B2 (ru) |
BR (1) | BR0015114A (ru) |
CA (1) | CA2389064A1 (ru) |
CZ (1) | CZ20021449A3 (ru) |
EE (1) | EE200200225A (ru) |
HU (1) | HUP0203704A3 (ru) |
IL (1) | IL148973A0 (ru) |
MX (1) | MXPA02003417A (ru) |
NO (1) | NO20021931D0 (ru) |
PL (1) | PL355487A1 (ru) |
RU (1) | RU2002114044A (ru) |
WO (1) | WO2001032890A1 (ru) |
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DE4005150A1 (de) | 1990-02-17 | 1991-08-22 | Hoechst Ag | Verfahren zur enzymatischen racematspaltung von pantolacton |
JP3011449B2 (ja) * | 1990-10-05 | 2000-02-21 | 富士薬品工業株式会社 | D―パントラクトン加水分解酵素およびその製造法 |
EP0507278A3 (en) | 1991-04-02 | 1993-12-15 | Hoechst Ag | Immobilised biocatalyser, its preparation and use for ester synthesis in a column reactor |
DE4126580A1 (de) | 1991-08-12 | 1993-02-18 | Degussa | D-2,4-dihydroxy-3,3-dimethylbutansaeurenitril, seine herstellung und verwendung |
WO1994009175A1 (en) | 1992-10-15 | 1994-04-28 | Kawasaki Steel Corporation | Method of continuously carburizing metal strip |
US6406898B1 (en) * | 1995-09-13 | 2002-06-18 | Daiichi Fine Chemical Co., Ltd. | D-pantolactone hydrolase and gene encoding the same |
WO2000028043A2 (en) | 1998-11-10 | 2000-05-18 | Novozymes Biotech, Inc. | Polypeptides having lactonohydrolase activity and nucleic acids encoding same |
US6395529B1 (en) * | 1998-11-10 | 2002-05-28 | Novozymes Biotech, Inc. | Polypeptides having lactonohydrolase activity and nucleic acids encoding same |
-
2000
- 2000-10-20 RU RU2002114044/13A patent/RU2002114044A/ru not_active Application Discontinuation
- 2000-10-20 JP JP2001535572A patent/JP2003530080A/ja not_active Withdrawn
- 2000-10-20 IL IL14897300A patent/IL148973A0/xx unknown
- 2000-10-20 CZ CZ20021449A patent/CZ20021449A3/cs unknown
- 2000-10-20 KR KR1020027005466A patent/KR20020043254A/ko not_active Application Discontinuation
- 2000-10-20 WO PCT/EP2000/010320 patent/WO2001032890A1/de not_active Application Discontinuation
- 2000-10-20 CA CA002389064A patent/CA2389064A1/en not_active Abandoned
- 2000-10-20 AU AU11416/01A patent/AU782517B2/en not_active Ceased
- 2000-10-20 HU HU0203704A patent/HUP0203704A3/hu unknown
- 2000-10-20 MX MXPA02003417A patent/MXPA02003417A/es unknown
- 2000-10-20 CN CN00814937A patent/CN1384880A/zh active Pending
- 2000-10-20 EP EP00972817A patent/EP1224296A1/de not_active Withdrawn
- 2000-10-20 EE EEP200200225A patent/EE200200225A/xx unknown
- 2000-10-20 BR BR0015114-9A patent/BR0015114A/pt not_active IP Right Cessation
- 2000-10-20 PL PL00355487A patent/PL355487A1/xx not_active Application Discontinuation
- 2000-10-20 US US10/111,451 patent/US6998258B1/en not_active Expired - Fee Related
-
2002
- 2002-04-24 NO NO20021931A patent/NO20021931D0/no not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
IL148973A0 (en) | 2002-11-10 |
BR0015114A (pt) | 2002-07-16 |
WO2001032890A1 (de) | 2001-05-10 |
NO20021931L (no) | 2002-04-24 |
EP1224296A1 (de) | 2002-07-24 |
CN1384880A (zh) | 2002-12-11 |
AU782517B2 (en) | 2005-08-04 |
CA2389064A1 (en) | 2001-05-10 |
NO20021931D0 (no) | 2002-04-24 |
KR20020043254A (ko) | 2002-06-08 |
AU1141601A (en) | 2001-05-14 |
PL355487A1 (en) | 2004-05-04 |
EE200200225A (et) | 2003-06-16 |
MXPA02003417A (es) | 2002-08-20 |
CZ20021449A3 (cs) | 2002-10-16 |
US6998258B1 (en) | 2006-02-14 |
HUP0203704A2 (hu) | 2003-03-28 |
JP2003530080A (ja) | 2003-10-14 |
HUP0203704A3 (en) | 2006-03-28 |
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