PT3083958T - Métodos de integração genómica - Google Patents
Métodos de integração genómicaInfo
- Publication number
- PT3083958T PT3083958T PT14831130T PT14831130T PT3083958T PT 3083958 T PT3083958 T PT 3083958T PT 14831130 T PT14831130 T PT 14831130T PT 14831130 T PT14831130 T PT 14831130T PT 3083958 T PT3083958 T PT 3083958T
- Authority
- PT
- Portugal
- Prior art keywords
- methods
- genomic integration
- genomic
- integration
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361918625P | 2013-12-19 | 2013-12-19 | |
| US201461937444P | 2014-02-07 | 2014-02-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT3083958T true PT3083958T (pt) | 2019-06-27 |
Family
ID=52432914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT14831130T PT3083958T (pt) | 2013-12-19 | 2014-12-19 | Métodos de integração genómica |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US9476065B2 (pt) |
| EP (1) | EP3083958B1 (pt) |
| JP (1) | JP6652489B2 (pt) |
| KR (1) | KR102274445B1 (pt) |
| CN (1) | CN106029886B (pt) |
| AU (1) | AU2014368982B2 (pt) |
| CA (1) | CA2933902C (pt) |
| MX (1) | MX2016007797A (pt) |
| PT (1) | PT3083958T (pt) |
| WO (1) | WO2015095804A1 (pt) |
Families Citing this family (82)
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| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| SG11201508028QA (en) | 2013-04-16 | 2015-10-29 | Regeneron Pharma | Targeted modification of rat genome |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| KR102380245B1 (ko) | 2013-11-07 | 2022-03-30 | 에디타스 메디신, 인코포레이티드 | 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물 |
| CN105980568B (zh) | 2013-12-11 | 2019-12-03 | 瑞泽恩制药公司 | 用于靶向修饰基因组的方法和组合物 |
| US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
| WO2015184268A1 (en) | 2014-05-30 | 2015-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods of delivering treatments for latent viral infections |
| SG10201803444YA (en) | 2014-06-23 | 2018-06-28 | Regeneron Pharma | Nuclease-mediated dna assembly |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| CN113444747A (zh) | 2014-11-21 | 2021-09-28 | 瑞泽恩制药公司 | 使用成对向导rna进行靶向遗传修饰的方法和组合物 |
| CN107208113A (zh) | 2014-12-19 | 2017-09-26 | 瑞泽恩制药公司 | 用于通过单步多重靶向进行靶向遗传修饰的方法和组合物 |
| DK3242949T3 (da) | 2015-01-06 | 2022-01-24 | Dsm Ip Assets Bv | Crispr-cas-system til en gærværtscelle |
| GB2531454A (en) * | 2016-01-10 | 2016-04-20 | Snipr Technologies Ltd | Recombinogenic nucleic acid strands in situ |
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| JP7409773B2 (ja) * | 2015-07-31 | 2024-01-09 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | 改変された細胞および治療の方法 |
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| CN105469123A (zh) * | 2015-12-30 | 2016-04-06 | 华东理工大学 | 一种基于k平面回归的缺失数据补全方法 |
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- 2014-12-19 KR KR1020167019329A patent/KR102274445B1/ko active IP Right Grant
- 2014-12-19 CN CN201480075698.1A patent/CN106029886B/zh active Active
- 2014-12-19 MX MX2016007797A patent/MX2016007797A/es active IP Right Grant
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- 2014-12-19 AU AU2014368982A patent/AU2014368982B2/en active Active
- 2014-12-19 CA CA2933902A patent/CA2933902C/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| US20150184199A1 (en) | 2015-07-02 |
| AU2014368982A1 (en) | 2016-06-16 |
| US20190017074A1 (en) | 2019-01-17 |
| CN106029886A (zh) | 2016-10-12 |
| MX2016007797A (es) | 2016-09-07 |
| US11390888B2 (en) | 2022-07-19 |
| AU2014368982B2 (en) | 2021-03-25 |
| US9476065B2 (en) | 2016-10-25 |
| JP6652489B2 (ja) | 2020-02-26 |
| CA2933902A1 (en) | 2015-06-25 |
| KR102274445B1 (ko) | 2021-07-08 |
| EP3083958B1 (en) | 2019-04-17 |
| CN106029886B (zh) | 2021-02-05 |
| KR20160098481A (ko) | 2016-08-18 |
| CA2933902C (en) | 2022-08-30 |
| EP3083958A1 (en) | 2016-10-26 |
| US20170058299A1 (en) | 2017-03-02 |
| JP2017500038A (ja) | 2017-01-05 |
| US20200354746A1 (en) | 2020-11-12 |
| US10041092B2 (en) | 2018-08-07 |
| WO2015095804A1 (en) | 2015-06-25 |
| US20220325303A1 (en) | 2022-10-13 |
| US10626418B2 (en) | 2020-04-21 |
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