PT1078051E - Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas - Google Patents

Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas Download PDF

Info

Publication number
PT1078051E
PT1078051E PT99922306T PT99922306T PT1078051E PT 1078051 E PT1078051 E PT 1078051E PT 99922306 T PT99922306 T PT 99922306T PT 99922306 T PT99922306 T PT 99922306T PT 1078051 E PT1078051 E PT 1078051E
Authority
PT
Portugal
Prior art keywords
virus
selection
polypeptide
phage
display system
Prior art date
Application number
PT99922306T
Other languages
English (en)
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of PT1078051E publication Critical patent/PT1078051E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Air Bags (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Vehicle Body Suspensions (AREA)
  • Circuits Of Receivers In General (AREA)
PT99922306T 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas PT1078051E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system

Publications (1)

Publication Number Publication Date
PT1078051E true PT1078051E (pt) 2008-03-17

Family

ID=26313668

Family Applications (1)

Application Number Title Priority Date Filing Date
PT99922306T PT1078051E (pt) 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas

Country Status (14)

Country Link
US (2) US7442159B1 (pt)
EP (2) EP1950295A1 (pt)
JP (2) JP4493846B2 (pt)
AT (1) ATE380865T1 (pt)
AU (1) AU762814B2 (pt)
CA (1) CA2328422A1 (pt)
CY (1) CY1107293T1 (pt)
DE (1) DE69937735T2 (pt)
DK (1) DK1078051T3 (pt)
ES (1) ES2299244T3 (pt)
GB (1) GB2361237A (pt)
NO (1) NO329172B1 (pt)
PT (1) PT1078051E (pt)
WO (1) WO1999058655A2 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2334170A1 (en) 1998-07-27 2000-02-10 Genentech, Inc. Improved transformation efficiency in phage display through modification of a coat protein
ES2327382T3 (es) 1999-07-20 2009-10-29 Morphosys Ag Metodos para presentar (poli)peptidos/proteinas en particulas de bacteriofagos a traves de enlaces disulfuro.
GB2375112A (en) * 2000-02-03 2002-11-06 Domantis Ltd Combinatorial protein domains
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
WO2002012277A2 (en) * 2000-08-07 2002-02-14 Domantis Limited Hybrid combinatorial proteins made from reshuffling of differently folded domains
DE10135039C1 (de) * 2001-07-18 2003-03-13 Nemod Immuntherapie Ag Verfahren zur Isolierung großer Varianzen spezifischer Moleküle für ein Zielmolekül aus Phagemid-Gen-Bibliotheken
WO2004101790A1 (en) 2003-05-14 2004-11-25 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
DK1902131T3 (da) 2005-07-08 2010-03-01 Univ Zuerich Fag-display under anvendelse af cotranslational translokation af fusionspolypeptider
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
US20090305898A1 (en) * 2005-07-21 2009-12-10 Ilan Morad Methods and Compositions for Identifying a Peptide Having an Intermolecular Interaction With a Target of Interest
GB0515115D0 (en) 2005-07-22 2005-08-31 Isogenica Ltd Peptide characterisation
CA2655933C (en) * 2006-06-23 2014-09-09 Alethia Biotherapeutics Inc. Polynucleotides and polypeptide sequences involved in cancer
CA2688434A1 (en) 2007-06-06 2008-12-11 Domantis Limited Polypeptides, antibody variable domains and antagonists
US9062097B2 (en) 2007-08-21 2015-06-23 Morpho Sys AG Methods for the formation of disulphide bonds
EP2615115A3 (en) 2007-11-30 2014-01-08 Glaxo Group Limited Antigen-binding constructs
CA3050455A1 (en) 2008-11-03 2010-06-03 Adc Therapeutics Sa Antibodies that specifically block the biological activity of a tumor antigen
CN102307897B (zh) 2008-12-05 2016-01-20 葛兰素集团有限公司 选出蛋白酶抗性多肽的方法
CA2753332A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
JP2012518400A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 多価および／または複数特異的ｒａｎｋｌ結合性構築物
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
EP2853542A1 (en) 2010-11-24 2015-04-01 Glaxo Group Limited Multispecific antigen binding proteins targeting HGF
PT3173427T (pt) 2011-03-31 2019-09-17 Adc Therapeutics Sa Anticorpos contra antigénio associado ao rim 1 e fragmentos de ligação a antigénio dos mesmos
JP5987057B2 (ja) 2011-07-27 2016-09-06 グラクソ グループ リミテッドＧｌａｘｏ Ｇｒｏｕｐ Ｌｉｍｉｔｅｄ Ｆｃドメインと融合した抗ｖｅｇｆ単一可変ドメイン
EP2771456B1 (en) * 2011-10-27 2018-08-15 The Governors of the University of Alberta Device and method for bacterial culture and assay
NZ625758A (en) 2012-01-09 2016-05-27 Alethia Biotherapeutics Inc Method for treating breast cancer
JP6915987B2 (ja) 2013-09-25 2021-08-11 シトムクス セラピューティクス，インコーポレイティド マトリックスメタロプロテイナーゼ基質及び他の切断可能部分並びにそれらの使用方法
IL302642A (en) 2014-01-31 2023-07-01 Cytomx Therapeutics Inc Polypeptide substrates that activate matriptase and U-plasminogen and other cleavable groups, preparations containing them and their uses
US20180340174A1 (en) * 2014-11-11 2018-11-29 Nanocore Aps Method for identification of molecules with desired characteristics
MA41374A (fr) 2015-01-20 2017-11-28 Cytomx Therapeutics Inc Substrats clivables par métalloprotéase matricielle et clivables par sérine protéase et procédés d'utilisation de ceux-ci
KR20170132154A (ko) * 2015-03-31 2017-12-01 브이에이치스퀘어드 리미티드 프로테아제-절단가능 링커를 갖는 펩티드 구조물
EP3987041A1 (en) * 2019-06-21 2022-04-27 Boehringer Ingelheim International Gmbh Novel mechanism to control rna virus replication and gene expression

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JP2919890B2 (ja) 1988-11-11 1999-07-19 メディカル リサーチ カウンスル 単一ドメインリガンド、そのリガンドからなる受容体、その製造方法、ならびにそのリガンドおよび受容体の使用
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
EP0585287B1 (en) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
CA2105300C (en) * 1991-03-01 2008-12-23 Robert C. Ladner Process for the development of binding mini-proteins
DE4122599C2 (de) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
PT1024191E (pt) 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos

Also Published As

Publication number Publication date
DE69937735D1 (de) 2008-01-24
AU3941399A (en) 1999-11-29
CY1107293T1 (el) 2012-11-21
NO20005628L (no) 2001-01-12
DE69937735T2 (de) 2008-11-27
GB2361237A (en) 2001-10-17
JP2010148507A (ja) 2010-07-08
ES2299244T3 (es) 2008-05-16
EP1078051B1 (en) 2007-12-12
WO1999058655A3 (en) 2000-01-27
WO1999058655A2 (en) 1999-11-18
JP2002514413A (ja) 2002-05-21
JP4493846B2 (ja) 2010-06-30
NO20005628D0 (no) 2000-11-08
EP1950295A1 (en) 2008-07-30
GB0026143D0 (en) 2000-12-13
US7442159B1 (en) 2008-10-28
US20090203542A1 (en) 2009-08-13
AU762814B2 (en) 2003-07-03
EP1078051A2 (en) 2001-02-28
CA2328422A1 (en) 1999-11-18
US8680018B2 (en) 2014-03-25
JP5161865B2 (ja) 2013-03-13
NO329172B1 (no) 2010-09-06
ATE380865T1 (de) 2007-12-15
DK1078051T3 (da) 2008-04-07

Similar Documents

Publication Publication Date Title
PT1078051E (pt) Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas
NZ521396A (en) Fusion proteins comprising two or more Neisseria polypeptides
IL121614A0 (en) System for the expression of heterologous antigens as fusion proteins
NZ506086A (en) Tumor-associated antigen derivatives from the mage family, and nucleic acid sequences encoding them, used for the preparation of fusion proteins, antigens comprising derivatised thiol residues and of compositions for vaccination
CA2245006A1 (en) Protein expression system
CA2237296A1 (en) Process for the preparation of peptides by way of streptavidin fusion proteins
IN190327B (pt)
WO2000006717A3 (en) Improved transformation efficiency in phage display through modification of a coat protein
AU1686395A (en) (deltex) proteins, nucleic acids, and antibodies, and related methods and compositions
AU2002300207A1 (en) Recombinant Rhabdovirus containing a heterologous Fusion Protein
DK0977873T3 (da) Fremgangsmåde til spaltning af fusionsproteiner
AU1692595A (en) Mammalian expression systems for hepatitis c virus envelope genes
WO2002022662A3 (en) Insect inhibitory bacillus thuringiensis proteins, fusions, and methods of use therefor
WO2001004331A3 (en) Enhanced expression of proteins using gfp
WO1999003993A3 (en) Suppressors of cytokine signaling; related reagents
WO2000018909A3 (en) NUCLEIC ACIDS AND PROTEINS FROM $i(CENARCHAEUM SYMBIOSUM)
WO2000031127A3 (en) Protein fragments for use in protein targeting
AU7649996A (en) Fused DNA sequence, fused protein expressed from said fused DNA sequence and method for expressing said protein
DE69534178D1 (en) S?ugetier thymokingene
AU2569588A (en) A method for the selective cleavage of fusion proteins
DE60040789D1 (de) Verfahren zur aufreinigung heterologer proteine
ZA200007619B (en) New peptide fragments for protein purification.
WO2000034335A3 (en) 'death domain' containing receptors
AU2003226907A1 (en) Method for the preparation of recombinant mammalian heparin-binding protein (hbp)
WO2001035255A3 (en) Large scale comparative protein structure modeling