PL410286A1 - Nowa metoda oczyszczania chitynazy A przy użyciu chromatografii powinowactwa - Google Patents
Nowa metoda oczyszczania chitynazy A przy użyciu chromatografii powinowactwaInfo
- Publication number
- PL410286A1 PL410286A1 PL410286A PL41028614A PL410286A1 PL 410286 A1 PL410286 A1 PL 410286A1 PL 410286 A PL410286 A PL 410286A PL 41028614 A PL41028614 A PL 41028614A PL 410286 A1 PL410286 A1 PL 410286A1
- Authority
- PL
- Poland
- Prior art keywords
- chitinase
- purification
- protein
- affinity chromatography
- new method
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2442—Chitinase (3.2.1.14)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01014—Chitinase (3.2.1.14)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Ujawniono kompleks białkowy zawierający białko G i związaną z nim niekowalencyjnie chitynazę A lub białko zawierające fragment chitynazy A mający powinowactwo do białka G oraz sposób wyodrębniania z roztworu wodnego chitynazy A lub białka zawierającego jej fragment mający powinowactwo do białka G, a także zastosowanie sposobu.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL410286A PL235436B1 (pl) | 2014-11-28 | 2014-11-28 | Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania |
PCT/IB2015/056710 WO2016083911A1 (en) | 2014-11-28 | 2015-09-03 | A novel method of chitinase a purification by affinity chromatography |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL410286A PL235436B1 (pl) | 2014-11-28 | 2014-11-28 | Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania |
Publications (2)
Publication Number | Publication Date |
---|---|
PL410286A1 true PL410286A1 (pl) | 2016-06-06 |
PL235436B1 PL235436B1 (pl) | 2020-08-10 |
Family
ID=54325568
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL410286A PL235436B1 (pl) | 2014-11-28 | 2014-11-28 | Kompleks białkowy zawierający białko G i chitynazę A, sposób wyodrębniania z roztworu wodnego chitynazy A oraz jego zastosowania |
Country Status (2)
Country | Link |
---|---|
PL (1) | PL235436B1 (pl) |
WO (1) | WO2016083911A1 (pl) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3862007A (en) * | 1973-03-15 | 1975-01-21 | Canadian Patents Dev | Extraction of chitinase |
US20030017570A1 (en) | 1996-06-14 | 2003-01-23 | Patrick W. Gray | Chitinase materials and methods |
JP3118573B1 (ja) | 1999-10-27 | 2000-12-18 | 工業技術院長 | キチナーゼ及びその製造法 |
-
2014
- 2014-11-28 PL PL410286A patent/PL235436B1/pl unknown
-
2015
- 2015-09-03 WO PCT/IB2015/056710 patent/WO2016083911A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2016083911A1 (en) | 2016-06-02 |
PL235436B1 (pl) | 2020-08-10 |
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