PL400501A1 - Sposób izolowania DNA drobnoustrojów z krwi - Google Patents
Sposób izolowania DNA drobnoustrojów z krwiInfo
- Publication number
- PL400501A1 PL400501A1 PL400501A PL40050112A PL400501A1 PL 400501 A1 PL400501 A1 PL 400501A1 PL 400501 A PL400501 A PL 400501A PL 40050112 A PL40050112 A PL 40050112A PL 400501 A1 PL400501 A1 PL 400501A1
- Authority
- PL
- Poland
- Prior art keywords
- blood
- microorganisms
- isolating dna
- lysis
- dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Abstract
Sposób umozliwia jednoczesne izolowanie DNA bakteryjnego i grzybiczego z krwi. W sposobie wykorzystuje sie lize enzymatyczna, lizy mechaniczna oraz lize termiczna.
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL400501A PL219490B1 (pl) | 2012-08-24 | 2012-08-24 | Sposób izolowania DNA drobnoustrojów z krwi |
ES13774254.0T ES2652267T3 (es) | 2012-08-24 | 2013-08-23 | Método para el aislamiento eficiente del ADN microbiano de la sangre |
PCT/PL2013/000109 WO2014031018A1 (en) | 2012-08-24 | 2013-08-23 | Method for efficient isolation of microbial dna from blood |
EP13774254.0A EP2888364B1 (en) | 2012-08-24 | 2013-08-23 | Method for efficient isolation of microbial dna from blood |
US14/422,860 US9879311B2 (en) | 2012-08-24 | 2013-08-23 | Method for efficient isolation of microbial DNA from blood |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL400501A PL219490B1 (pl) | 2012-08-24 | 2012-08-24 | Sposób izolowania DNA drobnoustrojów z krwi |
Publications (2)
Publication Number | Publication Date |
---|---|
PL400501A1 true PL400501A1 (pl) | 2014-03-03 |
PL219490B1 PL219490B1 (pl) | 2015-05-29 |
Family
ID=49322673
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL400501A PL219490B1 (pl) | 2012-08-24 | 2012-08-24 | Sposób izolowania DNA drobnoustrojów z krwi |
Country Status (5)
Country | Link |
---|---|
US (1) | US9879311B2 (pl) |
EP (1) | EP2888364B1 (pl) |
ES (1) | ES2652267T3 (pl) |
PL (1) | PL219490B1 (pl) |
WO (1) | WO2014031018A1 (pl) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3035409B1 (fr) * | 2015-04-21 | 2020-01-24 | Fondation Mediterranee Infection | Methode d'extraction d'adn comprenant un traitement de degradation enzymatique d'un echantillon biologique |
US10428370B2 (en) * | 2016-09-15 | 2019-10-01 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
US11959125B2 (en) | 2016-09-15 | 2024-04-16 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
US20200370036A1 (en) * | 2017-08-04 | 2020-11-26 | Microbedx, Inc. | Methods for Lysis of Cells Within a Sample |
CN113637668A (zh) * | 2021-10-15 | 2021-11-12 | 微岩医学科技(北京)有限公司 | 一种同时提取血浆和血细胞病原菌dna的试剂盒及应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU717453B2 (en) * | 1995-08-17 | 2000-03-23 | Eberhard-Karls-Universitat Tubingen Universitatsklinikum | Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material |
US6605439B2 (en) | 1995-08-17 | 2003-08-12 | Eberhard-Karls Universität Tübingen Universitätsklinikum | Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material |
EP2290099B1 (en) | 1998-02-02 | 2012-10-10 | QIAGEN North American Holdings, Inc. | Processes for isolating, amplifying and characterizing DNA |
US20020068280A1 (en) | 2000-12-06 | 2002-06-06 | Jeff Fairman | Compositions and methods for DNA purification from whole blood |
US8481265B2 (en) * | 2007-08-02 | 2013-07-09 | Universite Laval | Concentration and enrichment of microbial cells and microbial nucleic acids from bodily fluids |
US20090081678A1 (en) | 2007-09-21 | 2009-03-26 | Streck, Inc. | Nucleic acid isolation in preserved whole blood |
US10041061B2 (en) * | 2010-09-29 | 2018-08-07 | Ibis Biosciences, Inc. | Fungal nucleic acid extraction |
-
2012
- 2012-08-24 PL PL400501A patent/PL219490B1/pl unknown
-
2013
- 2013-08-23 WO PCT/PL2013/000109 patent/WO2014031018A1/en active Application Filing
- 2013-08-23 EP EP13774254.0A patent/EP2888364B1/en active Active
- 2013-08-23 US US14/422,860 patent/US9879311B2/en active Active
- 2013-08-23 ES ES13774254.0T patent/ES2652267T3/es active Active
Also Published As
Publication number | Publication date |
---|---|
EP2888364A1 (en) | 2015-07-01 |
ES2652267T3 (es) | 2018-02-01 |
US9879311B2 (en) | 2018-01-30 |
PL219490B1 (pl) | 2015-05-29 |
US20150240290A1 (en) | 2015-08-27 |
WO2014031018A1 (en) | 2014-02-27 |
EP2888364B1 (en) | 2017-11-15 |
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