PL1856257T3 - Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności - Google Patents

Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności

Info

Publication number
PL1856257T3
PL1856257T3 PL06716196T PL06716196T PL1856257T3 PL 1856257 T3 PL1856257 T3 PL 1856257T3 PL 06716196 T PL06716196 T PL 06716196T PL 06716196 T PL06716196 T PL 06716196T PL 1856257 T3 PL1856257 T3 PL 1856257T3
Authority
PL
Poland
Prior art keywords
dual specificity
specificity oligonucleotide
processes
oligonucleotide
dual
Prior art date
Application number
PL06716196T
Other languages
English (en)
Inventor
Jong-Yoon Chun
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/KR2005/001206 external-priority patent/WO2006095941A1/en
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of PL1856257T3 publication Critical patent/PL1856257T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/117Modifications characterised by incorporating modified base
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/125Allele specific primer extension

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
PL06716196T 2005-03-05 2006-03-03 Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności PL1856257T3 (pl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20050018419 2005-03-05
PCT/KR2005/001206 WO2006095941A1 (en) 2005-03-05 2005-04-26 Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
EP06716196A EP1856257B1 (en) 2005-03-05 2006-03-03 Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
PCT/KR2006/000746 WO2006095981A1 (en) 2005-03-05 2006-03-03 Processes using dual specificity oligonucleotide and dual specificity oligonucleotide

Publications (1)

Publication Number Publication Date
PL1856257T3 true PL1856257T3 (pl) 2012-03-30

Family

ID=36953549

Family Applications (1)

Application Number Title Priority Date Filing Date
PL06716196T PL1856257T3 (pl) 2005-03-05 2006-03-03 Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności

Country Status (7)

Country Link
EP (2) EP2365079A1 (pl)
JP (1) JP5619702B2 (pl)
KR (4) KR100977186B1 (pl)
PL (1) PL1856257T3 (pl)
PT (1) PT1856257E (pl)
RU (1) RU2400538C2 (pl)
WO (1) WO2006095981A1 (pl)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8871469B1 (en) * 2004-11-13 2014-10-28 Steven Albert Benner Self-avoiding molecular recognition systems in DNA priming
ES2400805T3 (es) * 2006-05-04 2013-04-12 Seegene, Inc. Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida
ATE543828T1 (de) * 2006-12-26 2012-02-15 Siemens Healthcare Diagnostics Heteropolynukleotid-duplexe mit purin-purin- paarung
WO2010026488A2 (en) 2008-09-03 2010-03-11 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
EP3075867B1 (en) * 2008-09-03 2019-03-13 Quantumdx Group Limited Sensing strategies and methods for nucleic acid detection using biosensors
WO2010026490A1 (en) 2008-09-03 2010-03-11 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
KR20110050327A (ko) 2009-11-07 2011-05-13 주식회사 씨젠 Thd 프라이머 타겟 검출
KR101569476B1 (ko) 2009-12-21 2015-11-16 주식회사 씨젠 Tsg프라이머 타겟 검출
JP5709897B2 (ja) * 2009-12-24 2015-04-30 シージーン アイエヌシー 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出
CA3113213A1 (en) 2010-01-12 2011-07-21 Siemens Healthcare Diagnostics Inc. Oligonucleotides and methods for detecting kras mutations
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
KR20120042100A (ko) 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
JP5922139B2 (ja) 2010-11-01 2016-05-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド エマルジョンを形成するためのシステム
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
EP2691543B1 (en) 2011-03-29 2017-11-01 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2012150749A1 (en) 2011-05-04 2012-11-08 Seegene, Inc. Detection of target nucleic acid sequences by po cleavage and hybridization
EP2737089B1 (en) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Library characterization by digital assay
CN102559661B (zh) * 2012-01-18 2014-06-04 厦门基科生物科技有限公司 一种新型连接酶反应介导的扩增方法及用途
NO2817421T3 (pl) * 2012-02-20 2018-05-12
CN104388426B (zh) * 2012-02-28 2017-08-08 盛司潼 一种Oligo dT引物及构建cDNA文库的方法
CN104245959B (zh) 2012-03-05 2017-10-13 Seegene株式会社 基于探测和标记寡核苷酸切割及延伸试验的从靶核酸序列的核苷酸变异检测
JP6544861B2 (ja) 2013-02-07 2019-07-17 ラトガース,ザ ステート ユニバーシティ オブ ニュー ジャージー 高い選択性の核酸増幅プライマー
EP2770065B1 (en) 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
JP2016508733A (ja) 2013-03-13 2016-03-24 シージーン アイエヌシー メルティングピーク分析を利用したターゲット核酸配列の定量
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ptoの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
CN105705657B (zh) 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
KR102016747B1 (ko) * 2014-12-09 2019-09-02 주식회사 씨젠 상이한 검출 온도 및 기준값을 사용한 타겟 핵산 서열의 검출
KR101742681B1 (ko) 2015-01-30 2017-06-01 에스디 바이오센서 주식회사 상보적 염기서열 내지는 미스-매치된 염기를 포함하는 상보적인 염기서열과 연결된 pcr 프라이머 및 이를 이용한 핵산 증폭 방법
WO2016137031A1 (ko) * 2015-02-25 2016-09-01 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
JP2019503174A (ja) 2016-01-04 2019-02-07 クァンタムディーエックス グループ リミテッドQuantumdx Group Limited 電荷量タグを含む合成ヌクレオチドのデザイン、合成、および使用
WO2017209575A1 (en) * 2016-06-03 2017-12-07 Seegene, Inc. Evaluation of specificity of oligonucleotides
KR101785687B1 (ko) * 2016-07-20 2017-10-17 (주)다이오진 다중 증폭 이중 시그널 증폭에 의한 타겟 핵산 서열의 검출 방법
CN111742056B (zh) * 2017-06-23 2023-09-01 荣研化学株式会社 核酸检测方法、核酸检测用引物以及核酸检测用试剂盒
EP3688188A4 (en) 2017-09-29 2021-06-16 Seegene, Inc. DETECTION OF TARGET NUCLEAR ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION DEPENDENT NON-HYBRIDIZATION TEST
WO2024054924A1 (en) * 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
EP0359789B1 (en) 1988-01-21 1993-08-04 Genentech, Inc. Amplification and detection of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5817797A (en) 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1990001069A1 (en) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
AU5269590A (en) 1989-03-10 1990-10-09 Gene-Trak Systems Immobilized oligonucleotide probes and uses therefor
AU635105B2 (en) 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5432065A (en) 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5831065A (en) 1994-04-04 1998-11-03 Lynx Therapeutics, Inc. Kits for DNA sequencing by stepwise ligation and cleavage
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US5723298A (en) 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US20030165888A1 (en) 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6013449A (en) 1997-11-26 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Probe-based analysis of heterozygous mutations using two-color labelling
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2004001042A2 (en) * 2002-06-20 2003-12-31 Nuevolution A/S Microarrays displaying encoded molecules
CA2468754C (en) * 2001-12-08 2011-11-15 Seegene, Inc. Annealing control primer and its uses
WO2003093509A1 (en) 2002-05-01 2003-11-13 Seegene, Inc. Methods and compositions for improving specificity of pcr amplication
CA2469923A1 (en) 2001-12-12 2003-06-19 Genset S.A. Biallelic markers of d-amino acid oxidase and uses thereof
ES2640279T3 (es) * 2002-10-30 2017-11-02 Nuevolution A/S Procedimiento para la síntesis de un complejo bifuncional
WO2004074429A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
FR2933490B1 (fr) 2008-07-02 2010-08-27 Commissariat Energie Atomique Procede de mesure du debit d'un liquide en ecoulement dans un canal fluidique et dispositif de mise en oeuvre

Also Published As

Publication number Publication date
KR101243266B1 (ko) 2013-03-25
EP1856257A1 (en) 2007-11-21
WO2006095981A1 (en) 2006-09-14
EP1856257B1 (en) 2011-10-19
KR100977186B1 (ko) 2010-08-23
RU2400538C2 (ru) 2010-09-27
EP2365079A1 (en) 2011-09-14
KR20090094388A (ko) 2009-09-04
KR20100099333A (ko) 2010-09-10
EP1856257A4 (en) 2009-02-18
PT1856257E (pt) 2012-01-02
JP5619702B2 (ja) 2014-11-05
KR20110122231A (ko) 2011-11-09
KR101032750B1 (ko) 2011-05-06
RU2007132657A (ru) 2009-04-10
JP2012065655A (ja) 2012-04-05
KR20070111543A (ko) 2007-11-21

Similar Documents

Publication Publication Date Title
PL1856257T3 (pl) Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności
EP1723162A4 (en) ANTI-microRNA oligonucleotide molecules
EP1968612A4 (en) NUCLEOTIDE AND OLIGONUCLEOTIDE PRODRUGS
GB0422052D0 (en) Enzymes
IL228770A0 (en) processes and intermediates
DE602006010048D1 (en) Biosensor
EP1791567A4 (en) CHEMICALLY MODIFIED OLIGONUCLEOTIDES
EP1969123A4 (en) NEW ENZYMES
EP1903334A4 (en) BIOSENSOR
ZA200703326B (en) Genomic assay
ZA200807747B (en) Catalyst and hydrotreting process
EP1909098A4 (en) BIOSENSOR
EP1859035A4 (en) IMMOBILIZED ENZYMES
PT1907574E (pt) Oligonucleótidos
GB0402487D0 (en) Catalytic process
EP1852499A4 (en) AGENT PROMOTING NEUROTISATION
GB0409771D0 (en) Assay
EP1908829A4 (en) OLIGONUCLEOTIDE PROBE
GB0519605D0 (en) Assay
GB0407526D0 (en) Assay
GB0427585D0 (en) Assay
GB0408853D0 (en) Assay
GB0511279D0 (en) Assay
GB0408559D0 (en) Assay
GB0503741D0 (en) Assay