NZ729518A - Biomarkers and combination therapies using oncolytic virus and immunomodulation - Google Patents

Abstract

Disclosed is a method for predicting the response of a patient, with cancer or is suspected of having cancer, to an oncolytic virus comprising the steps of: (a) determining the level of expression of Th1 and/or Th2 biomarkers and/or antibodies against tumor associated antigens in a test sample; (b) comparing the levels of expression (in (a)) to a control, wherein the change in expression level relative to the control sample is predictive of the patient’s treatment response to the oncolytic virus. Also disclosed are methods of treating cancer comprising administering an oncolytic virus and measuring the level of Th1/Th2 biomarkers at two different time points to determine the patients response to the treatment. Also disclosed is a method for treating cancer in a patient comprising the administration of Th1 cytokine, an oncolytic virus and an agent which suppresses regulatory T cells and/or stimulates a cell mediated immune response.

Description

PATENT APPLICATION BIOMARKERS AND COMBINATION THERAPIES USING ONCOLYTIC VIRUS AND IMMUNOMODULATION CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent ation No. 61/590,441, filed January 25, 2012 and U.S. Provisional Patent Application No. 61/637,191, filed April 23, 2012, the entire content of each of which is orated herein by reference.

BACKGROUND 1. Field of Invention The present invention relates generally to the fields of oncology and cancer therapy.

In some aspects, the present invention ns combination therapies including oncolytic Viruses such as adenoviruses and immune modulating therapies. In other aspects, the t invention concerns the ement or detection of biomarkers that distinguish responders from sponders to oncolytic Viral therapy. 11. Background It is estimated that 43,800 new cases of primary brain tumor, both malignant and nonmalignant, were sed in the U.S. in 2005. It was estimated at 20,500 primary malignant brain tumors including astrocytic (42%) were expected in 2007. This was a more male predominant disease with estimated 11,700 in men and 8,800 in women in 2007. There were approximately 12,700 people who died with brain disease from these tumors estimated in the US in 2007. These tumors account for 1.4% of all adult cancers and 22% of all childhood cancers.

This accounts for 2.4% of all cancer related deaths (SEER Cancer Statistics Review).

Onocolytic viruses have shown potential as anti-cancer agents. Genetic modification of the viruses to ively replicate in cancer cells further increases their efficacy. In gliomas, for example, three kinds of viruses have been shown to be useful in animal models: reoviruses that can replicate selectively in tumors with an activated ras y (Coffey et al., 1998); cally altered herpes simplex viruses (Martuza et al., 1991; Mineta et al., 1995; Andreanski et al., 1997), ing those that can be activated by the different expression of protein in normal and cancer cells (Chase et al., 1998); and mutant adenoviruses that are unable to express the E1B55 kDa protein and are used to treat p53—mutant tumors (Bischof et al., 1996; Heise et al., 1997; Freytag et al., 1998; Kim et al., 1998). In all three systems, the goal is the intratumoral spread of the Virus and the ability to selectively kill cancer cells. Genetically modified adenoviruses that target cellular pathways at key points have both potent and selective anti- cancer effects in gliomas. Frequently tested modifications of the adenovirus include deletion of the viral genes that interact with tumor ssor genes, the modification of the tropism to infect cancer cells with more y, and the inclusion in the viral genome of elements of transcription that are sensitive to transcription factors upregulated in cancer cells.

The role that preexisting immune conditions play and their influence in the overall clinical outcome of oncolytic virus therapy is presently unknown. The ability to accurately predict al in patients treated with oncolytic viruses such as 24-RGD would improve current treatment decisions. Furthermore, it would aid in the design of new therapies that may be tailored, with the basis of tumor properties and immune status. A significant e in the field of biotherapy would occur because there is presently a dramatic lack of clinical biomarkers for cancer immunotherapy strategies. 77 The need for therapies effective against primary tumors of the nervous system, such as diffuse gliomas, anaplastic ytomas, anaplastic oligodendrogliomas, anaplastic mixed oligoastrocytoma, glioblastoma, ependymomas, and stic ependymomas, or any y brain tumor is particularly acute.

SUMMARY The present invention relates to the identification of Thl matory) cytokines and antibodies against tumor associated antigens as biomarkers for correlating their expression patterns as predictors of responsiveness to ent with tic viruses in patients with . The invention thus provides for the identification and use of expression profiles which correlate with (and thus are able to discriminate between) patients with good or poor ent es. In several embodiments, the invention provides expression patterns that are able to identify patients with cancer that are likely to be non-responsive to treatment with an tic virus from those that are responsive or likely to be responsive to treatment with an oncolytic virus. Responsiveness may be viewed in terms of better survival outcomes over time. siveness may also be viewed in terms of reduction in tumor size according to e.g. the RECIST criteria.

The present invention also relates to novel methods for treating cancer patients comprising the co-administration of oncolytic virus and agents which stimulate a Th1 immune response in the patient and/or suppress a Th2 immune response and/or suppress regulatory T cells in the patient.

In one aspect, the present invention provides an objective means for identifying patients with cancer as likely to d, or not respond, to treatment with an oncolytic virus by assaying for the expression level of one or more of the biomarkers described herein. Expression of these biomarkers thus provides an objective means to determine cancer prognosis (treatment outcome) with significant accuracy. These biomarkers may be used in combination with subjective criteria.

The biomarkers described herein are identified as correlating with oncolytic virus ent outcome in patients with cancer such that their expression levels are relevant to determining appropriate treatment protocols. In one ment, the patient is a patient with a high grade glioma and the oncolytic virus is an irus such as DeltaRGD.

WO 12942 [01 l] The biomarkers described herein may be used singly with significant accuracy or in any combination, such as in the format of a ratio of expression levels, to increase the ability to accurately correlate an expression profile with a treatment outcome. The biomarkers can be used to t treatment responsiveness, survival outcome and determination and/or alteration of therapy. The ability to identify patients likely to respond to, or likely not to respond to, oncolytic Virus therapy is conferred by the identification of an expression level of the biomarkers and not by the methodology used to determine such expression level. Thus, the assay may utilize any feature of a biomarker described herein as long as the assay provides a ative or preferably a quantitative expression of the protein (or gene). By way of example, a biomarker can be measured or detected by a variety of methods, including, but not limited to immunohistochemistry (IHC), immunoassays, protein , reverse protein arrays, nucleic acid arrays, mass spectroscopy, polymerase chain reaction (PCR) and the like.

In several embodiments, a method for ting whether a t having or suspected of having cancer will respond therapeutically to a method of treating cancer comprising administering an oncolytic virus is provided comprising the steps of (a) determining the level of expression of one or more Thl biomarkers and/or one or more Th2 kers and/or one or more antibodies against one or more tumor associated antigens in a test sample from the patient ve to a control (b) comparing the level of expression of one or more Thl biomarkers and/or one or more Th2 biomarkers and/or one or more antibodies against one or more tumor associated antigens in the test sample to that in the control, wherein a change in the level of expression of one or more Thl and/or one or more Th2 kers and/or antibodies t one or more tumor associated antigens in the test sample ve to the control sample is predictive of the patient’s treatment response to the tic virus. The test samples include but are not limited to blood, plasma, serum, tissue biopsy, and ospinal fluid. In related embodiments, the method comprises determining the level of at least one Thl biomarker and the level of antibodies against at least one tumor associated antigen in step (a) and comparing these levels to a control in step (b).

The expression level of the one or more biomarkers can be determined prior to, simultaneous with or after administration of an oncolytic virus in order to t the outcome of the therapy. In one embodiment, a method for ng cancer in a patient is provided comprising (a) determining the level of sion of one or more Thl biomarkers and/or one or more Th2 biomarkers and/or one or more antibodies against one or more tumor associated antigens in a test sample from the patient obtained prior to administration of an oncolytic virus relative to a control (b) comparing the level of expression of one or more Thl biomarkers and/or one or more Th2 biomarkers and/or one or more antibodies against one or more tumor associated ns in the test sample to that in the control, wherein a change in the level of expression of one or more Thl and/or one or more Th2 kers and/or antibodies against one or more tumor associated antigens in the test sample relative to the control sample is predictive of the patient’s treatment response to the oncolytic virus and optionally (c) administering the oncolytic virus to the patient if the patient is determined to be likely to respond to treatment with the virus. In a preferred embodiment, oncolytic virus is an adenovirus such as DeltaRGD, the test sample is a serum sample and the levels IL~12p70 and optionally at least one additional Thl biomarker are determined in the sample and compared to a control.

In a related embodiment, the patient is determined as unlikely to respond to the oncolytic virus treatment if low levels of one or more Thl biomarkers (e.g. IL—12p70) and/or high levels of antibodies against one or more tumor associated antigens (e.g. NLRP4) are determined in the test sample relative to a control. For example, step (b) as described above may se detecting the level of one or more Th1 biomarkers and/or antibodies t one or more tumor ated antigens in the test sample ve to the level of one or more Thl biomarkers and/or antibodies against one or more tumor associated antigens from a t having the same cancer that is sive to treatment with the oncolytic virus, wherein lower levels of one or more Thl biomarkers and/or higher levels of antibodies against one or more tumor associated antigens in the subject having the same cancer that is responsive to treatment indicates that the patient is non-responsive to treatment with the oncolytic virus. Preferably, the test sample from the patient and the sample from the subject are taken at the same time point.

Thus, the sion pattern of the biomarkers described herein is useful for the identification of patients presenting with cancer that are unlikely to respond to treatment with an oncolytic virus.

These patients are thus identified as good candidates for co—administration of agents which stimulate a Th1 immune response with an oncolytic virus. Alternatively, these patients are identified as good candidates for alternative treatment ties.

In another d embodiment, the patient is determined as likely to respond to oncolytic virus treatment if high levels of one or more Thl biomarkers (e.g. 70) and/or low levels of antibodies against one or more tumor associated antigens (e.g. NLRP4) are determined in the test sample ve to a control. For example, step (b) as described above may comprise detecting the level of one or more Thl biomarkers and/or antibodies against one or more tumor associated antigens in the test sample relative to the level of one or more Th1 biomarkers and/or antibodies against one or more tumor associated antigens from a subject having the same cancer that is not responsive to treatment with the oncolytic virus, wherein higher levels of one or more Thl biomarkers and/or lower levels of antibodies against one or more tumor associated antigens in the subject having the same cancer that is not responsive to treatment indicates that the patient is responsive to treatment with the tic virus.

Preferably, the test sample from the patient and the sample from the subject are taken at the same time point. Thus, the expression pattern of the biomarkers described herein is useful for the identification of patients presenting with cancer that that are likely to respond to treatment with an oncolytic virus. These patients are good candidates for stration of the oncolytic virus and may be administered the virus.

In other embodiments, a method for evaluating a t with cancer for responsiveness to oncolytic viral therapy is provided comprising (a) ing or detecting one or more biomarkers indicative of the immunologic status of the subject in a test sample from the subject (b) fying, based on the levels of the one or more kers, a subject having cancer that is likely or not likely to respond to the therapy, wherein a favorable response is likely if the immune status of the subject indicates a Th1 polarization and optionally (c) administering the subject an oncolytic Viral therapy if a ble response is likely. In n aspects, the biomarker is a cytokine, cell surface marker, or antibody.

In one aspect, a method for predicting the likelihood that a subject will respond therapeutically to a method of treating cancer by administering an oncolytic virus comprises the following steps: (a) measuring the sion level of at least one biomarker suitable for determining the Thl and/or Th2 immunologic status of the subject prior to administration of the virus (b) comparing the expression level of step (a) to a predetermined l, whereby an se in one or biomarkers of Thl immune status relative to the control and/or a decrease in one or more biomarkers of Th2 immune status relative to the control indicates an increased likelihood that the subject will respond therapeutically to said method of treating cancer and optionally (c) administering an oncolytic virus to the subject if an increased likelihood of se is ted.

In another aspect, the expression level of one or more biomarkers in a patient with cancer is assessed at le time points as a means of predicting the patient’s clinical outcome wherein the patient is undergoing oncolytic virus therapy. This method comprises measuring at least one Thl biomarker and/or antibodies against at least one tumor associated antigen in two or more test samples from the patient over a period of time in order to determine whether a t should continue treatment with the oncolytic virus or alternatively whether the t is a candidate for co-administration of an agent to produce a Thl ype. A decrease in at least one Thl ker and/or an increase in antibodies against one or more tumor associated antigens compared to the level of the same Thl biomarker(s) and/or antibodies at an earlier time point indicates that the patient is at risk for a negative outcome. Alternatively, a decrease in at least one Thl biomarker and/or an increase in antibodies against one or more tumor associated antigens compared to the level of the same Thl ker(s) and/or antibodies in another patient undergoing the same treatment tes that the patient is at risk for a negative outcome. Thus, in one embodiment, a method for treating cancer in a patient having or suspected of having cancer is provided comprising (a) administering an oncolytic virus (b) measuring the level of one or more Th1 kers and/or antibodies against one or more tumor associated antigens at a first time point and at a subsequent second time point and (c) comparing the level of one or more Thl biomarkers and/or antibodies against one or more tumor antigens at the first time point and the second time point wherein a se in one or more Thl biomarkers and/or an increase in antibodies against one or more tumor antigens at the second time point compared to the first time point or compared to the level of the same Th1 biomarkers and/or dies in another patient with the same cancer and administered the same oncolytic virus indicates that the patient is non- responsive to treatment with the oncolytic Virus. Onocolytic virus therapy may be discontinued in patients fied as non-responsive to the virus; alternatively, one or more agents that produce a Th1 phenotype may be co-administered to the patient with the virus.

In one aspect, biomarkers of Th1 immune status (i.e. Thl biomarkers) e, without limitation, immunomodulators such as IL-lB, IL—2, IL—8, IL-12, IL-18, IFN-y, TNF-oc, TNF-B, GM-CSF, cleaved caspase-3, rin, and B2-microglobulin. Th1 biomarkers may be used alone or in any combination according to the methods bed herein. In a preferred embodiment, the expression of at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11 or all 12 Thl biomarkers selected from the group consisting of 1L1 B, IL—2, IL-8, IL—12, IL-18, IFN—y, TNF-oc, TNF-B, GM-CSF, cleaved caspase—3, neopterin, and [32 microglobulin is measured in a test sample from a patient. In a particularly preferred embodiment, the expression of IL-12 and ally at least 1, at least 2, at least 3 at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 biomarkers ed from the group consisting of ILlB, IL—2, IL—8, IL—l8, IFN-y, TNF-Ot, TNF-B, GM—CSF, cleaved caspase-3, neopterin, and B2 microglobulin is measured in a test sample from a patient. In other preferred embodiments, the expression of at least 1, 2, 3, 4, 5, 6, 7 or all 8 Th1 biomarkers selected from the group consisting of ILlB, lL-2, IL-8, IL—12 IFN—y, TNF-oc, GM- CSF, and d caspase-3 is measured in a test sample from a patient. ed expression of any (and preferably of the majority or all) of these biomarkers relative to a predetermined l indicates an increased hood that the patient will respond to oncolytic virus therapy. It should be understood that if, for example 10 Th1 biomarkers are assessed in a test sample from the patient, a high level of 1, 2, 3, 4, 5, 6, 7, 8, or 9 of these biomarkers, in any combination, or a high level of all 10 of these biomarkers, indicates that the patient is likely to respond favorably to oncolytic virus therapy.

In other s, biomarkers of Th2 immune status (i.e. Th2 biomarkers) include, without limitation, IL-4, IL-5, IL—6, IL-10, IL-13, TGF—B and phosphorylated STAT3. Th2 biomarkers may be used alone or in any combination according to the methods described herein.

Preferably, at least one, at least 2, 3, 4, 5, or at least 6 Th2 biomarkers selected from the group consisting of IL—4, IL—5, IL-6, IL—10, IL-l3, TGF-B and phosphorylated STAT3 are measured in a test sample from a patient. It should be understood that if, for example 5 Th2 biomarkers are assessed in a test sample from the patient, a high level of l, 2, 3, or 4 of these biomarkers, in any combination, or a high level of all 5 of these biomarkers, indicates that the patient is unlikely to d favorably to oncolytic virus therapy.

In related embodiments, the expression of at least 1, 2, 3, 4, 5, 6, 7, or all 8 Thl kers selected from the group consisting of ILlB, IL—2, IL-8, IL-l2, IFN-y, TNF-oc, GM- CSF and cleaved e-3, and at least one, at least two or all three Th2 biomarkers selected from the group consisting of IL—6, IL—lO and phosphorylated STAT3 (Tyr 705) are measured in a test sample from a patient according to the methods of the invention. In a ularly preferred embodiment, the methods comprise measuring the expression of IL-12 and optionally at least 1, 2, 3, 4, 5, 6, or all 7 biomarkers selected from the group consisting of ILlB, IL-2, IL-8, IFN—y, GM-CSF, TNF-oc and cleaved caspase-3, and measuring the expression of at least 1, 2, or all 3 biomarkers selected from the group ting of IL—6, IL-10 and phosphorylated STAT3 (Tyr 705). Elevated expression of any (and preferably all) of the Thl kers and similar or decreased expression of any (and preferably all) of the Th2 biomarkers relative to a predetermined controls indicates an increased hood that the t will respond to oncolytic virus y. Preferably, the expression of the Thl biomarkers and the Th2 biomarkers is calculated and a ratio of Thl/Th2 biomarker expression is calculated, whereby a ratio higher than that of a ermined control indicates an increased likelihood that the subject will respond to oncolytic virus therapy. For example, a ratio above 0.2, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 5.0 or more indicates an sed likelihood that the subject will respond to oncolytic Virus therapy. In a related embodiment, the method comprises measuring the expression of IL-12 and optionally IL-6 or IL-lO in a test sample from the subject, wherein an increased ratio of IL-12/IL—6 and/or IL-l2/IL-10 expression relative to a predetermined control indicates an increased likelihood that the subject will respond to oncolytic virus therapy In another aspect, biomarkers of Thl immune status include cell surface markers ing, without limitation, CXCR3, CCRS, CCR1 and IL-12 receptor [31 and CL chains. In other aspects, biomarkers of Th2 immune status include, without limitation, cell surface markers including, without limitation, CXCR4, CCR3, CCR4, CCR7, CCR8, IL-1 receptor and CD30.

In another aspect, biomarkers that may be measured as a means for determining the immune status of a t with cancer include without limitation, antibodies against tumor associated ns (e.g. cancer/testis ns) selected from the group ting of BRAF, CABYR, CRISP3, CSAG2, CTAG2, DHFR, FTHL17, GAGEl, LDHC, MAGEAl, MAGEA3, MAGEA4, MAGEB6, MAPKl, MICA, MUCl, NLRP4, NYESOl, P53, PBK, FRAME, SOX2, SPANXAl, SSX2, SSX4, SSXS, TSGAlO, TSSK6, TULP2, XAGE2, and ZNF165. Anti-tumor associated antigen antibody biomarkers may be used alone or in any combination according to the methods described herein. Thus, the expression level of antibodies against at least 1, at least 2, at least 3, 4, 5, 6, 7, 8,9,10,11,12,l3,14,15,16,17,18,l9, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or at least 30 tumor associated antigens selected from the group ting of: BRAF, CABYR, CRISP3, CSAG2, CTAG2, DHFR, FTHL17, GAGEl, LDHC, , MAGEA3, MAGEA4, MAGEB6, MAPKl, MICA, MUCl, NLRP4, , P53, PBK, FRAME, SOX2, l, SSX2, SSX4, SSXS, TSGAlO, TSSK6, TULP2, XAGE2, and ZNF165 may be measured in a test sample according to the methods described herein. Preferably, the sion level of antibodies against at least one, at least 2, 3, 4, 5, 6 7, 8, or at least 9 tumor associated ns selected from the group consisting of: CABYR, , MAGEA3, MAGEB6, NLRP4, NYESOl, PBK, SSX2, SSXS, and ZNF165 is measured in a test sample from the patient. In related embodiments, the expression level of antibodies against NLRP4 and optionally at least one, 2, 3, 4, 5, 6, 7, or at least 8 tumor associated antigens selected from the group consisting of CABYR, MAGEAl, MAGEA3, MAGEB6, NYESOl, PBK, SSX2, SSXS, and ZNF165 is measured in a test sample from the subject. A high level of expression of one or more of these tumor associated antigens compared to a control level indicates that the patient is unlikely to respond to oncolytic Virus therapy. It should be understood that if, for example antibodies against 9 tumor associated antigens rkers) are assessed in a test sample from the patient, a high level of 1, 2, 3, 4, 5, 6, 7 or 8 of these biomarkers, in any combination, or a high level of all 9 of these biomarkers, indicates that the patient is unlikely to respond favorably to oncolytic Virus therapy.

In related embodiments, the expression of at least 1, 2, 3, 4, 5, 6, 7, or all 8 Th1 biomarkers selected from the group consisting of ILlB, IL-2, IL-8, IL-12, IFN—y, TNF-oc, GM- CSF and cleaved caspase—3, and the expression of antibodies against at least 1, at least 2, at least 3, 4, 5, 6, 7, 8, 9,10,11,12,13,14,15,16,17,18,19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or at least 30 tumor associated antigens selected from the group consisting of: BRAF, CABYR, CRISPS, CSAG2, CTAG2, DHFR, FTHL17, GAGEI, LDHC, , MAGEA3, MAGEA4, MAGEB6, MAPKI, MICA, MUCl, NLRP4, NYESOl, P53, PBK, PRAME, SOX2, SPANXAl, SSX2, SSX4, SSXS, TSGAIO, TSSK6, TULP2, XAGE2, and ZNF165 are ed in a test sample from a patient according to the methods of the invention. In a particularly preferred embodiment, the methods comprise measuring the sion of IL-12 and optionally at least 1, 2, 3, 4, 5, 6, or all 7 biomarkers selected from the group consisting of ILIB, IL-2, IL-8, IFN—y, GM-CSF, TNF-oc and cleaved caspase—3, and measuring the expression of antibodies against at least one, at least 2, 3, 4, 5, 6 7, 8, or at least 9 tumor associated antigens selected from the group consisting of: CABYR, MAGEAl, MAGEA3, MAGEB6, NLRP4, NYESOl, PBK, SSX2, SSXS, and ZNF165. ed expression of any (and preferably all) of the Th1 kers and/or decreased sion of any (and preferably all) of the antibodies against tumor associated antigens relative to a predetermined controls indicates an increased likelihood that the subject will respond to oncolytic Virus therapy.

In a preferred ment, the test sample obtained from the patient is a serum sample and the level of IL-12 and ally at least one additional Th1 ker is determined e.g. by ELISA. Alternatively or onally the level of at least one Th2 biomarker and/or the level of antibody against NLRP4 and optionally at least one additional tumor associated antigen is measured in a serum sample from the patient.

In yet another aspect, the immunologic status of the patient is determined by measuring the level of one or more Thl biomarkers and the level of antibodies against one or more tumor antigens as described above in a test sample (e. g. serum) from a t prior to or during tic virus therapy. Thus, a patient with a low level of one or more Thl biomarkers (e.g. IL-12) and a high level of antibodies against one or more tumor antigens (e.g. NLRP4) compared to a control level of Thl biomarkers and antibodies against one or more tumor antigens is unlikely to respond to oncolytic virus therapy. Conversely, a patient with a high level of one or more Thl biomarkers and a low level of antibodies t one or more tumor antigens compared to a control level is likely to respond favorably to oncolytic virus therapy.

In other related aspects, the method comprises ing lymphocytes from a tissue sample from the subject. In one aspect, the concentration of CD4+ and CD8+ T cells is measured (e.g. by flow tric analysis following treatment with anti-CD4 and anti-CD8 antibodies) and a ratio of CD8+/CD4+ cells in the test sample is calculated, whereby an sed ratio of CD8+/CD4+ cells in the test sample relative to a predetermined control indicates an sed likelihood that the subject will respond to oncolytic virus therapy. In another , lymphocytes are ed from a tissue sample from the subject and a ratio of FoxP3'/F0XP3+ cells is calculated, whereby an sed ratio of FoxP3'/FoxP3+ cells in the test sample relative to a ermined control indicates an increased likelihood that the subject will respond to oncolytic virus therapy. FoxP3 is predominantly expressed on and therefore serves as a marker for regulatory T cells which act to suppress cell mediated immunity. Optionally, FoxP3 is measured along with CD25 and CD4 as a method for determining the level of regulatory T cells.

CD4+, CD8+ or regulatory T cells can be detected for example with anti-FoxP3, anti-CD4, anti- CDS, anti—CD38, and anti—HLA-DR antibodies. Surface marker profiles c for T cell subsets are known in the art.

In d aspects, the method comprises measuring in at least one test sample from a subject (i) the percentage of CD4+ cells, CD8+ cells and optionally FOXP3+ cells relative to total lymphocytes in the sample and (ii) the level of antibodies against at least one tumor associated antigen and/or (iii) the level of at least one Thl biomarker, whereby a percentage of CD4+ and/or FoxP3+ cells greater than 50, 60, 70, 80 or 90% of total lymphocytes and a high level of dy against at least one tumor associated antigen and/or a low level of Thl biomarker relative to a predetermined control indicates that the subject is unlikely to respond to oncolytic viral therapy.

Conversely, a higher percentage of CD8+ cells relative to CD4Jr and/or FoxP3+ cells and/or a low level of antibody against at least one tumor associated antigen and/or a high level of Th1 biomarker relative to a predetermined control indicates that the subject is likely to respond to oncolytic viral therapy.

In another embodiment, a method for treating cancer in a subject is provided comprising the following steps: (a) administering to the subject an oncolytic Virus for a treatment period (b) ing one or more Th1 and/or Th2 biomarkers in a test sample from the subject at least twice during the treatment period and (c)(l) co~administering a Th1 stimulating agent with the oncolytic virus or (c)(2) tinuing administration of the oncolytic Virus if a decrease in the level of one or more Th1 biomarkers is detected. Optionally, one or more Thl and/or Th2 biomarkers are measured in a test sample just before or at the beginning of the treatment period in order to provide a ne measurement. A reduction in one or more Thl biomarkers during the treatment period indicates a need for either co—administration of a Thl stimulating agent with the virus or termination of oncolytic virus therapy, particularly if the level of one or more Th2 kers is not reduced (e.g. remains substantially the same or increases).

Test samples ed from a subject according to the methods, e without limitation, one or more samples obtained from tissue (e.g. tumor biopsy), cerebrospinal fluid (CSF), lymph, blood, plasma, serum, peripheral blood mononuclear cells (PBMCs), lymph fluid, lymphocytes, synovial fluid and urine. In particular embodiments, the test sample is obtained from CSF or tumor . In other particular embodiments, the test sample is ed from tumor tissue and e.g. the ve number of CD4+ and/or CD8+ cells in the sample is ined and/or the level of one or more Thl and/or Th2 cytokines in the sample is measured e.g. by immunofluorescent staining of fixed and permeabilized cells from the sample with antibodies against the Thl and/or Th2 cytokines. In other particular embodiments, the test sample is obtained from blood and e.g. the level of one or more Thl and/or Th2 cytokines in the sample is measured by ELISA.

In a broad aspect, the present invention also provides a ation therapy for treating cancer in a patient comprising co-administering the t an oncolytic virus and one or more agents that produce a Thl immune phenotype. This methodology of using both oncolytic s (e.g. iruses) and immune stimulatory agents is counterintuitive since activating the immune system could ially reduce the level of oncolysis produced by viruses such as DeltaRGD. An activated immune system would be expected to clear the virus at an accelerated rate and thus reduce the effectiveness of the oncolytic virus therapy. Indeed, ce suggests that slowing the development of Thl ses is important for the y of oncolytic therapy. The present inventors, however, have singly discovered that the patients who exhibit strong tumor responses radiographically as well as prolonged survival show virus replication, immune stimulation, and activation of T-cell mediated cytotoxicity. Contran'wise, the present inventors have surprisingly discovered that patients demonstrating a relatively strong Th1 profile are more likely to t a positive response to oncolytic therapy.

The agent that produces a Thl immune phenotype in the patient can be administered prior to, during or after administration of the oncolytic virus. In one embodiment, a patient with cancer that is determined to be at risk for not responding favorably to oncolytic virus therapy according to any of the s fore described, is co—administered with the virus one or more Thl stimulating agents in an amount sufficient to increase the level of one or more Thl biomarkers of the invention and/or decrease the level of one or more Th2 biomarkers and/or decrease the level of antibodies against one or more tumor associated antigens. In a preferred embodiment, the patient is administered one or more agents that produce a Th1 immune phenotype prior to administration of the oncolytic virus in order to "prime" the patient’s immune system to respond favorably to oncolytic virus therapy and therefore increase the likelihood that the patient will have a favorable clinical outcome. In another embodiment, a patient with cancer that is determined to be likely to respond favorably to oncolytic virus therapy according to any of the s heretofore described, is co-administered with the virus one or more Thl stimulating agents in an amount ent to increase the level of one or more Thl biomarkers of the invention and/or decrease the level of one or more Th2 biomarkers and/or decrease the level of antibodies against one or more tumor ated antigens. In a preferred embodiment, the patient is administered one or more agents that produce a Th1 immune phenotype prior to administration of the oncolytic virus in order "prime" the patient’s immune system in order to boost the anti- tumor response to the oncolytic virus.

Certain embodiments are directed to s of treating cancer in a t comprising administering (i) a replication competent oncolytic virus (e.g. adenovirus), and (ii) an agent that upregulates or activates the cellular immune system. The agent can be administered prior to, during or subsequent to administration of the virus. The upregulation or activation of the cellular immune system can be accomplished by either stimulating the cellular immune system or suppressing the inhibition of the cellular immune system. The methods can be used for the treatment of primary tumors or tumors formed from metastasis. In certain aspects, the virus further comprises a targeting moiety. In further aspects, the ation competent Virus is an adenovirus such as delta-24. In still a further aspect, the delta-24 adenovirus ses a targeting moiety. In certain s the targeting moiety is an RGD containing peptide. In certain aspects, the RGD or other naturally occurring cell surface binding peptide s an immune privilege to the oncolytic virus enabling the virus to remain therapeutic during times of elevated immune system ty.

In one preferred embodiment, a method for treating cancer in a patient is provided comprising: (a) administering to the patient a cytokine (e.g. e.g. recombinant 70 or recombinant IFN—y) or one or more agents (e.g. Revlimid or lenalidomide) to increase the production of Th1 cytokines such as 70 or IFN-y (b) administering to the patient an oncolytic virus (e.g. DeltaRGD) and optionally (c) administering to the patient an agent to suppress regulatory T cells (e.g. temozolomide, cyclophosphamide, CCNU, BCNU, melphalan, busulfan) and/or stimulate a cell mediated immune response (e.g. Ipilimumab, Tremelimumab, MDX-1106, MK-3475, AMP-224, Pidilizumab, MDX-llOS). ably the tic Virus is stered by intratumoral injection into one or multiple areas of the tumor. The patient may have been previously determined by methods of the invention to be likely to d to oncolytic virus therapy or alternatively may have been previously determined to be unlikely to respond to oncolytic virus therapy. Preferably, the cytokine or agent to increase production of Th1 cytokines is administered prior to the oncolytic virus and the agent to suppress regulatory T cells and/or stimulate a cellular immune repsonse is administered during or subsequent to oncolytic virus therapy. In a related embodiment, step (a) further or alternatively comprises administering one or more agents to suppress the production of Th2 cytokines such as IL-1 0 and IL-4.

In certain aspects the agent that produces a Th1 phenotype is administered to the patient 1, 2, 3, 4, 5, 6, 7, ,11,12,13,14,15,16,17,18,19, 20, 21, 22, 23, 24 hours, 1, 2, 3, 4, 5, 6, 7 or more days, 1, 2, 3, 4 weeks or 1, 2, 3, 4, 5, 6 months, including all values and ranges there between, prior to or after administration of the tic Virus. In a preferred embodiment, the agent that that produces a Th1 phenotype is administered prior to administration of the oncolytic virus and optionally is ued after administration of the virus.

In one embodiment, the agent that that produces a Th1 phenotype is stered to the patient between 1 and 14 days prior to or after administration of the oncolytic Virus. In another embodiment, the agent is administered n 1 and 4 weeks prior to or after administration of the oncolytic virus. In other embodiments, the agent is simultaneously co-administered to the patient with the oncolytic virus.

In certain aspects, an assessment of cellular immune function will be carried out prior to, , or after administration of the oncolytic virus or the immune activation agent e. g. by measuring the level of one or more Th1 and/or Th2 biomarkers as described herein and/or antibodies to one or more tumor related antigens in the blood. Alternatively, immune cell infiltrates such as antigen presenting cells (e.g. hages, dendritic cells, astrocytes and microglia), cytotoxic T cells, or natural killer cells can be detected in biopsy samples from a tumor.

In one aspect, a concentration of tumor infiltrating CD4+ and CD8Jr lymphocytes is assessed, wherein a patient with a high CD4+/CD8+ ratio compared to a l (indicating a Th2 ) is simultaneously, separately or consecutively inistered a replication competent oncolytic virus and an agent that upregulates or activates the cellular immune system. In another aspect, the ve number of Thl/Th2 CD4+ cells in a test sample (e. g. biopsy or peripheral blood mononuclear cells) from the subject is determined by measuring co-expression of CD4 and one more Thl cytokines (e.g. IL-12p70 or IFN—y) and Th2 nes (e. g. IL-4) and determining the relative number of Thl/Th2 CD4+ cells. Preferably, the cells are stimulated (e.g. With Phorbol ester plus Ionomycin), fixed, permeabilized, stained with appropriate antibodies and subjected to flow cytometric analysis. In one embodiment, one or more cytokine levels indicative of a Th1 profile can be measured in the blood. serum or other fluids. In one aspect, a patient with a low level of Thl cytokines compared to an appropriate control or a high level of Th2 cytokines compared to an riate control is inistered a replication competent oncolytic virus, and an agent that upregulates or activates the cellular immune system.

In a related embodiment, one or more Th1 cytokines ed from the group consisting of ILlB, IL-2, IL-8, IL-12, IL-18, IFN—y, TNF-oc, TNF-B and GM-CSF are measured.

Other Thl biomarkers such as cleaved caspase-3, neopterin and [32 lobulin may also be measured as part of a Thl profile. In r d embodiment, one or more Th2 cytokines selected from the group consisting of IL—4, IL—5, IL-6, IL-10 and IL-13 and TGF-B are measured.

Other Th2 biomarkers such as phosphorylated STAT3 (Tyr 705) may also be measured as part of a Th2 profile. In other aspects, cytokines or other kers indicative of a Th1 and/or Th2 profile are monitored during tic virus (e. g. adenovirus) therapy wherein a patient exhibiting a shift from a Th1 profile to a Th2 profile during therapy is administered an agent that upregulates or activates the cellular immune system. Therapy d necrosis can be detect and/or measured.

In certain embodiments the agent that produces a Th1 phenotype (upregulates or activates the cellular immune ) is an antagonist of a ssor of cellular immunity (antagonist of cellular immune-suppression). Antagonists of cellular immune-suppression are agents that act on cells or molecules that suppress the cellular immune system. Antagonists of cellular immune-suppression include cytotoxic T-lymphocyte antigen 4 (CTLA-4; also known as CD152) antagonists such as Ipilimumab (also known as YervoyTM, MDX-OIO or MDX-lOl; a humanized monoclonal antibody against CTLA-4 developed by Bristol-Myers Squibb) and Tremelimumab (formerly ticilimumab, CP-675,206; a humanized monoclonal antibody against CTLA-4 une/ AstraZeneca); PD-l/PD-Ll — receptor antagonists such as MDX-1106 (an a—PD-l humanized monoclonal antibody, Bristol-Myers Squibb); MK-3475 (an d-PD-l humanized onal antibody, Merck); AMP-224 (Fc—PD-l fusion protein that blocks interaction between PD—1 and ligands B7-DC and B7-H1; Glaxo Smith Kline); Pidilizumab (also known as CT-Oll; a humanized monoclonal antibody against dPD-l, Chirotech); MDX-1105 (an a-PD—Ll humanized monoclonal antibody, Bristol-Myers Squibb); antibodies that specifically bind to B7-H3 such as MGA271 (an d-B7-H3 zed monoclonal antibody, Microgenics) or other antibodies as described in US Application Publication Number 2012— 0294796, the contents of which are incorporated herein by reference; or amine—2,3— dioxygenase (lDO) inhibitors such as thyl-tryptophan (Lunate) and other compounds described in US Patent Number 7,799,776, the contents of which are incorporated herein by In certain embodiments, the agent that upregulates the cellular immune system is a cellular immune system stimulator. An immuno-stimulator is a small molecule, peptide, polypeptide (e.g. antibody), or cell that when introduced into a subject results in increases the activity of cell mediated immune response. Immuno-stimulators include co—stimulatory y agonists, such as CD137 agonists ing without limitation EMS-663513 (an a-CD137 humanized monoclonal dy agonist, Bristol-Myers Squibb); agonists to CD40, such as CP- 870,893 40 humanized monoclonal antibody, Pfizer); 0X40 ) agonists (e.g. anti— OX4O humanized monoclonal dies, AgonOx and those described in US Patent Number 7,959,925); or ts to CD27 such as CDX-1127 (Ct-CD27 humanized monoclonal antibody, Celldex).

In certain aspects, an immune-stimulator is an agent that induces or stimulates antigen-presenting cells relative to DeltaRGD antigens, such as nists of CD47 or SIRPoc by either monoclonal antibodies or small molecule inhibitors including without limitation h (Trillium Therapeutics Inc.) and other inhibitors as described in US Patent Publication Number 2012/0189625, the contents of which are incorporated herein by reference.

Small molecule inhibitors that t the immuno-silencing of tumors include inhibitors of JAK-2, JAK-3 STAT—3 or STAT-5 such , as Lestaurtinib , (CEP-701 hydrate, (9S, 1 OS, 1 2R)-2,3 ,9,1 0,1 exahydro—10-hydroxy-l0-(hydroxymethyl)—9-methy1—9,l2-epoxy— ndolo[1 ,2,3-fg:321kl]pyrrolo[3 ,4-i] [1 ,6]benzodiazocin-1 —one; Sigma—Aldrich; JAK-2 inhibitor), Pacritinib (SB1518; 1 1—(2—pyrrolidiny1-ethoxy)—14,19—dioxa-5,7,26-triaza- yclo[19.3.1.1(2,6).1(8,12)]heptacosa—1(25),2(26),3 ,5,8,10,12(27),16,21,23—decaene; JAK- 2/FLT3 inhibitor), Tofacitinib (CF-690550; 3—[(3R,4R)methyl-3 -[methyl(7H—pyrrolo[2,3- d]pyrimidin-4—yl)amino]piperidiny1] -3 -oxopropanenitri1e; JAK-3 inhibitor, Pfizer); Ruxolitinib ((3R)—3—cyclopenty1-3 -[4—(7H-pyrrolo[2,3-d]pyrimidinyl)pyrazol yl]propanenitrile; JAK- 1 /JAK-2 inhibitor, Incyte and Novartis); CYT3 87 (JAK-2 inhibitor; N- (Cyanomethy1)[2-(4—morpholinoanilino)pyrimidinyl]benzamide; YM BioSciences); Baricitinib (LY3009104; 2-[1-ethylsu1fonyl-3 - [4-(7H-pyrrolo [2,3 imidin—4-yl)pyrazol- l - yl] azetidin-3 -yl]acetonitrile; JAK- 1 /JAK-2 inhibitor); and TG101348 (N—tert—Butyl {5-methyl- 2-[4—(2-pyrrolidin- l -yl-ethoxy)-pheny1amino]-pyrimidinylamino} -benzenesulfonamide; JAK— 2 inhibitor).

In further aspects ts with cancer can be administered one or more ical modifiers that produce a Th1 phenotype (activate the cellular immune response) such as cytokines (preferably recombinant) including without limitation GM—CSF, IL—2, IL—12, lL-18 and interferon-y prior to or during oncolytic virus therapy in order to improve the patient’s response to the virus. lFN-y and IL-12 are known to inhibit production of Th2 cytokines and are therefore particularly preferred cytokines for activating the cellular immune response.

Alternatively, these patients can be administered one or more agents that stimulate production of IL—12p70 and/or other Th1 cytokines such as lenalidomide (Revlimid), or domide. In a related , these patients can alternatively or additionally be administered one or more agents that decrease T-regulatory cells such as alkylating agents including without limitation Temozolomide, cyclophosphamide, lomustine , bis-chloroethylnitrosourea (BCNU), melphalan hydrochloride, an (butane-1,4-diyl dimethanesulfonate), rethamine (nitrogen d), chlorambucil, ifosfamide, streptozocin, dacarbazine (DTIC), thiotepa, altretamine (hexamethylmelamine), cisplatin, carboplatin, and oxalaplatin, prior to, during or after oncolytic Virus therapy. In another related aspect, these ts can be administered one or more agents which neutralize Th2 cytokines such IL—4, IL-5, IL-6, IL-10 and IL—l3, particularly IL-4 and IL—10 prior to or during oncolytic Virus therapy; because these Th2 cytokines suppress the Th1 pathway, their neutralization should improve the patient’s response to the Virus.

The present inventors have discovered that administration of an alkylating agent such as temozolomide to a t with cancer prior to administering an oncolytic Virus such as Delta- 24-RGD singly increases the likelihood that the patient will respond favorably to the Virus.

Without being bound by theory, it is believed that lymphopenia induced in glioma patients following treatment with alkylating agents such as temozolomide may result in a shift in the tumor environment from a predominantly Th2 ype to a Th1 phenotype, y potentiating the tumor to treatment with an oncolytic virus. Thus, in a preferred embodiment of the invention, a method for treating cancer in a patient is provided comprising administering to the patient an alkylating agent and subsequently administering to the patient an tic Virus.

Preferably, the patient is a patient with a y or metastatic brain tumor such as a glioma, the virus is an irus such as Delta-24 or DeltaRGD, and the alkylating agent is selected from the group consisting of lomide, cyclophosphamide, lomustine (CCNU), bis- chloroethylnitrosourea (BCNU), melphalan hydrochloride and busulfan (butane-1,4—diyl dimethanesulfonate). In a particularly preferred ment, the alkylating agent is temozolomide. The alkylating agent can be administered 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15,16,17,18,19, 20, 21, 22, 23, 24 hours, 1, 2, 3, 4, 5, 6, 7 or more days, 1, 2, 3, 4 weeks or 1, 2, 3, 4, 5, 6 months, including all values and ranges there between, prior to administration of the oncolytic Virus.

In other embodiments, the oncolytic virus is co-administered with one or more adjuvants which promote a Th1 response nonlimiting examples of which e monophosphoryl lipid A (MPL®), QS-21 (plant extract comprising soluble triterpene glucoside compounds) or other saponin, oligodeoxynucleotides comprising or consisting of CpG, and ribosomal protein extract (RPE).

In certain aspects the cancer to be treated according to the present s is a cancer of the central nervous system. In related aspect, the cancer is a neuroepithelial tumor such as, Without tion, an astrocytic tumor (e.g. astrocytoma, stic ytoma, glioblastoma, gliosarcoma, pilocytic astrocytoma, giant cell astrocytoma, pleomorphic xanthoastrocytoma), an oligodendroglioma, an ependymoma, an oligoastrocytoma, a spongioblastoma, an astroblastoma, a choroid plexus papiloma, a choroid plexus carcinoma, a gangliocytoma, a ganglioglioma, a neurocytoma, a neuroepithelial tumor, a neuroblastoma, a pineal region tumor (such as a pineocytoma, a lastoma, or a mixed ytoma/pineobastoma), a medulloepithelioma, a medulloblastoma, a neuroblastoma or ganglioneuroblastoma, a retinoblastoma, or an ependymoblastoma. In another related aspect, the cancer is a central nervous system neoplasm such as, Without limitation, a tumor of the sellar region (such as a pituitary adenoma, a pituitary carcinoma, or a craniopharyngioma), a hematopoietic tumor (such as a primary malignant lymphoma, a plasmacytoma, or a granulocytic sarcoma), a germ cell tumor (such as a germinoma, an embryonal carcinoma, a yolk sac tumor, a choriocarcinoma, a ma or a mixed germ cell tumor), a meningioma, a mesenchymal tumor, melanocytoma, or a tumor of cranial or spinal nerves (such as a schwannoma, or a neurofibroma). In a particular aspect, the cancer is a low—grade glioma (e.g. ependymoma, astrocytoma, oligodendroglioma or mixed ) or high-grade nant) glioma (e.g. glioblastoma multiforme). In another aspect, the cancer is a primary or metastatic brain tumor. In other aspects, the cancer is a primary or atic brain tumor comprising brain tumor cell stem cells. In a preferred embodiment, the cancer is a malignant glioma comprising brain tumor cell stem cells.

In other s, cancers to be d according to the present methods include without limitation, lung, ovary, breast, cervix, pancreas, stomach, colon, skin, larynx, bladder, and prostate cancer. ably the cancer to be treated exhibits a disrupted Rb pathway. In yet other aspects, the oncolytic virus is administered to treat a hyperproliferative disorder such as metaplasias, dysplasias or hyperplasia.

In certain aspects, the oncolytic virus is an adenovirus such as Delta—24-RGD.

Oncolytic adenoviruses such as 24-RGD have the ability to replicate in a variety of cell lines including lung, breast, prostate, sarcomas and glioma stem-like cells. Thus, e.g. oncolytic adenoviruses such as Delta—24-RGD may be administered to a patient having any cancer sive to replication of the virus in order to treat the cancer.

BRIEF DESCRIPTION OF THE DRAWINGS Antibodies vs. Survival (Humoral vs Cellular skew 1. A graph depicting the correlation n antibodies t tumor associated ns and survival in patients with gliomas d with oncolytic adenovirus (DeltaRGD). Briefly, serum from a subset of 20 glioma patients was assessed prior to DeltaRGD therapy for antibodies against 31 tumor associated antigens including BRAF, CABYR, CRISP3, CSAG2, CTAGZ, DHFR, FTHL17, GAGEl, LDHC, MAGEAl, MAGEA3, MAGEA4, MAGEB6, MAPKI, MICA, MUCl, NLRP4, NYESOl, P53, PBK, PRAME, SOXZ, SPANXAl, SSXZ, SSX4, SSXS, TSGAlO, TSSK6, TULPZ, XAGEZ, and ZNF165 by automated protein microarray using a modified solid— phase ELISA etrix). Over 40% of patients with fewer than 15 ve antibodies survived more than 20 months after treatment whereas every patient with more than 15 positive antibodies did not survive past 11 months. er, those ts with fewer than 15 positive antibodies who did not survive more than 20 months experienced an improved clinical outcome relative to those having more than 15 positive antibodies.

FIG 2. Immunity to antigen NLRP4 vs. survival. A graph depicting immunity to tumor associated antigen NLRP4 as a function of survival in glioma ts treated with Delta- 24-RGD. Antibodies against NLRP4 were assessed in sera from glioma patients prior to administering the virus and at several time points thereafter generally in monthly intervals.

Patients K, O, I, E, L, N, Q, A, D and J, surviving between 1 month and 11 months post treatment, experienced a relatively high level of antibodies against NLRP4 whereas patients P, C, H, B, M and G, surviving from more than 12 months to more than 19 months post treatment, experienced very low to ctable levels of antibodies against NLRP4. Patient G exhibited a complete response to the therapy.

Correlation of anti-tumor associated antigen antibodies with tumor recurrence. The upper panel is a graph depicting the ation of antibodies against tumor antigens with tumor recurrence in a glioma t (Patient F). Antibodies against an array of tumor associated antigens (those bed in were measured in a serum sample from the patient at the end of DeltaRGD therapy and at 15 days, 1 month, 4 months and 6 months post therapy. No antibodies were detected at the 15 day or 1 month period. However, between the four month and six month period the number of positive antibodies jumped from two to twenty and coincided with recurrence of the tumor. The lower panel depicts a scan of Patient F at the end of Delta—24-RGD therapy (left) and at 6 months after therapy (right). The ence of tumor can be seen in the scan at the 6 month timepoint.

Comparison of Patients G and I. A heat map depicting the results of a peptide array to detect antibodies against a panel of tumor associated antigens (those bed in in sera from two glioma patients (Patient G, a complete responder (left panel), and Patient I who ssed (right panel)) treated with DeltaRGD taken prior to treatment with the virus (0 months) and one, four and six months after treatment (Patient G) and one, three and four months after treatment (Patient 1). The results depicted in the heat map are raw data ed from absorbance analysis (i.e. not background subtracted). An average background of ~0.2 was observed and accordingly a cutoff that could be applied universally to all ns for positive versus negative results was 3 x ound = 0.6. Patient G survived is presently still alive over 30 months after treatment with the Virus. Patient I deceased 6 months after therapy.

Patient G had a score of 0 at each time point, (negative for antibodies in the serum against all of the 31 tumor ated antigens tested). Patient I on the other hand had a score of 10 prior to ent with the virus (positive for dies against CABYR, MAGEAl, MAGEA3, MAGEB6, NLRP4, NYESOl, PBK, SSXZ, SSX5 and ZNF165) and this number increased to 18 at the one month time point, 22 at the three month time point and 31 (i.e. antibodies against all tumor associated antigens tested) at the four month time point. This clearly demonstrates the usefulness of these tumor antigens as biomarkers for predicting the likelihood of response to oncolytic virus therapy such as DeltaRGD.

A graph ing the results of the peptide analysis described in The upper panel illustrates the results of serum analysis from Patient G, a complete responder. The lower panel rates the results of serum analysis from Patient I who did not respond. Serum samples were obtained and analyzed for antibodies reactive against the ed tumor associated antigens pre-treatment (both patients) with the virus and one month, four months and six months after treatment with the Virus (Patient G) and one month, three months and four months after treatment with the Virus (Patient 1). Antibody levels are on the "y" axis; antigens are on the "x" axis. Patient G was negative for all 31 antibodies both prior to and after ent with the Virus. Patient H was ve for 10 dies prior to treatment with the virus and was positive for all 31 antibodies at the four month time point.

Brain scans from a glioma patient (Patient 30A) who exhibited very high levels of IL-l2p70 prior to treatment with DeltaRGD are depicted. The upper left scan demonstrates the glioma in the patient prior to administering Delta—42-RGD therapy (11/18/2011). The upper middle scan demonstrates a positive response in the tumor eight days after therapy (10/26/2011) and eleven days after y (10/29/2011). The bottom left scan demonstrates the response nearly two months after y (1/7/2012) and nearly three months after therapy (2/8/2012).

FIG 7. Measurement of IL—12p70 (in picograms/ml) in patient sera samples. Figure 7 is a graph (on a logarithmic scale) depicting IL-12p70 (picograms/ml) in sera of glioma patients treated with Delta-24—RGD prior to therapy p) and one month, two months and three months post treatment. Patients 12A, 30A and 33A had very high IL-12p70 levels at baseline (prior to treatment with the virus) and these levels increased after treatment with the Virus. Patients 12A and 30A and 33A showed a complete response to the Virus and this correlated with IL-l2p70 levels both prior to and after treatment (IL—12p70 levels were over 100- fold and over 1000-fold higher in these patients relative to non-responders). On the other hand, the remaining patients depicted on the graph ted low levels of IL-12p70 prior to and after stration of the virus and did not respond to the virus. Patient 37A (not shown on the graph) had low baseline IL—12p70 levels and was given IFN—y approximately 2 months after treatment with the virus to stimulate a Th1 immune response and increase IL-12p70 levels. stration of IFN—y to Patient 37A caused a significant increase in IL-12p70 levels (indicating a switch to a Th1 immune response) and corresponded with a complete response to the virus. Thus, IL-12p70 levels (6. g. pretreatment) correlate very well with treatment outcome, with responders having high levels of IL-12 both prior to and during therapy. Conversely, non- responders had low levels of IL—12 prior to and during therapy. Strikingly, administration of the Th1 stimulating agent IFN-y to a patient exhibiting low IL-12p70 levels boosted IL-12p70 levels in the patient and a complete response to the virus was observed in the patient.

DESCRIPTION Definitions As used herein, the term "antigen" is a molecule capable of being bound by an antibody or T-cell receptor. An antigen is additionally e of inducing a humoral immune response and/or cellular immune response leading to the production of B- and/0r T— cytes. The structural aspect of an antigen, e.g., three dimensional conformation or modification (e. g., phosphorylation) that gives rise to a biological se is ed to herein as an "antigenic determinant" or pe. B-lymphocytes d to n antigenic determinants via antibody production, whereas T-lymphocytes are the mediator of cellular immunity. Thus, antigenic determinants or epitopes are those parts of an n that are ized by antibodies, or in the context of an MHC, by T-cell receptors. An antigenic inant need not be a contiguous sequence or segment of protein and may include various sequences that are not immediately adjacent to one another. In certain aspects, Tau oligomers are utilized as antigens.

The term "antibody" or "immunoglobulin" is used to include intact dies and binding fragments/segments thereof. Typically, fragments compete with the intact antibody from which they were derived for specific binding to an antigen. Fragments include separate heavy chains, light chains Fab, Fab', F(ab')2, Fabc, and Fv. Fragments/segments are produced by recombinant DNA techniques, or by enzymatic or chemical separation of intact immunoglobulins. The term "antibody" also includes one or more immunoglobulin chains that are chemically conjugated to, or expressed as, fusion proteins with other proteins. The term "antibody" also includes a bispecific antibody. A bispecific or bifunctional antibody is an artificial hybrid antibody having two different light chain pairs and two different binding sites. Bispecific antibodies can be ed by a variety of methods including fusion of omas or linking of Fab’ fragments. See, e. g., Songsivilai & nn (1990); Kostelny et a1. ( 1992).

In one aspect, the term "control" or "predetermined control" as used herein refers to a baseline level of a Thl and/or Th2 biomarker and/or antibody against a tumor associated antigen as determined from one, or preferably an average obtained from more than one, "normal" or "healthy" subjects believed not to or confirmed by diagnosis not to have a cancer. Once a level has become established for a standard population, results from test samples can be directly ed with the predetermined control. For example, a baseline may be obtained from at least one subject and preferably is obtained from an average of subjects, wherein the subject or subjects have no prior history of cancer. By way of example, a level of a Thl ker such as IL—12p70 in a serum sample from a patient may be compared to the serum level of the same Thl biomarker in an undiseased subject or an average serum level of more than one undiseased subjects.

In another aspect, the term ol" or "predetermined control" refers to a baseline level of a Thl and/or Th2 biomarker and/or antibody against a tumor associated antigen as determined from an e value in subjects having the same type of cancer as the patient to be d. By way of example, a level of a Thl ker such as 1L12p70 in a serum sample from a patient may be compared to an average serum level obtained from multiple subjects with the same type of cancer as the t to be treated. Glioma patients generally exhibit 70 serum levels in the range of 10-20 picograms/ml.

In a related aspect, the term "control" or "predetermined control" refers to a level of a Thl and/or Th2 biomarker and/or antibody against a tumor associated antigen in one subject, or preferably an average level of more than one subject, having the same type of cancer as the patient to be treated who have been administered the same oncolytic virus and shown to have not responded to the virus. For example, a level of a Thl cytokine such as IL-12p70 in a serum sample from the patient is compared to an average serum level obtained from more than t with the same type of cancer who did not d to the virus, whereby a high level of the Thl cytokine compared to the average level from non-responders tes that the patient is likely to respond favorably to the virus.

In another related , the term "control" or "predetermined control" refers to a level of biomarker in one subject, or preferably an average level of more than one t, having the same type of cancer as the patient to be treated who have been administered the same oncolytic virus and shown to have responded well to the virus. For example, a level of a Thl cytokine such as IL—12p70 in a serum sample from the patient is compared to an average serum level obtained from more than subject with the same type of cancer who responded well to the virus, whereby a low level of the Thl cytokine compared to the average level from good responders indicates that the patient is unlikely to respond favorably to the virus.

For purposes of comparison, the level of Th1 and/or Th2 biomarker and/or antibody t a tumor associated antigen in a test sample to be measured is of the same type (obtained from the same biological source) and is processed in the same way as what is used for determination of the baseline control level. For example, if a level of IL-12p70 is determined in by measuring the level of IL-12p70 in serum, the baseline level of IL—12p70 is determined by measuring the level of IL—12p70 in serum from e. g. normal healthy subjects. As used herein, a "high" level or an "increase" in the measured level of a Thl biomarker relative to a predetermined control means that the amount or concentration of Thl biomarker in a test sample is sufficiently greater in the test sample ve to the predetermined control level of Th1 biomarker. For example, an increase in the level of a Thl biomarker relative to a ermined control may be any statistically significant increase which is detectable such as without limitation, about a 1%, about a 5%, about a 10%, about a 15%, about a 20%, about a 30%, about a 40%, about a 60%, about an 80%, about a , about a 3-fold, about a 5-fold, about an 8- fold, about a 10—fold, about a 20-fold 50-fold, ld, ZOO-fold, SOD—fold or even 1000-fold elevation or more relative to the ermined control. In another aspect, a "high level" of biomarker in a test sample compared to control may refer to a detection level of an dy above a predetermined threshold wherein the control level is below the predetermined threshold.

The term "correlate" or "correlation" or equivalents thereof refer to an ation between expression of one or more genes or proteins and a treatment outcome of a cancer cell and/or cancer patient in comparison to the lack of response. The invention provides for the correlation between increases in expression of one or more of the herein disclosed biomarkers and responsiveness of a cancer patient to oncolytic virus therapy.

The term "glioma" refers to a tumor originating in the neuroglia of the brain or spinal cord. s are derived from the glial cell types such as astrocytes and oligodendrocytes, thus gliomas include astrocytomas and endrogliomas, as well as anaplastic gliomas, glioblastomas, and ependymomas. Astrocytomas and ependymomas can occur in all areas of the brain and spinal cord in both children and . endrogliomas typically occur in the cerebral hemispheres of . Other brain tumors are iomas, ependymomas, pineal region tumors, choroid plexus tumors, neuroepithelial tumors, embryonal tumors, peripheral neuroblastic tumors, tumors of cranial nerves, tumors of the hemopoietic system, germ cell , and tumors of the stellar .

The term "IL-12p70" or "IL-12" refers to the biologically active form of IL-12, a 70 kDa (p70) cytokine produced mainly be monocytes, macrophages, B-lymphocytes and dendritic cells. IL-12 is a heterodimer composed of two subunits, one 40 kDa (p40) and the other 35 kDa (p35) linked together by disulfide bonds.

As used herein the term "norma " in the context of a diagnosis or prognosis refers to an individual or group of individuals who have not shown any symptoms of cancer and are not known to suffer from the disorder. Preferably, the normal individual(s) is not on medication for treating cancer and if possible has not been diagnosed with cancer or any other hyperproliferative disorder. "Normal" according to the invention also refers to samples isolated from normal individuals.

The term "oncolytic virus" refers generally to any virus capable of replicating in and killing tumor cells. Preferably, the virus is engineered e.g. to increase tumor cell selectivity.

Representative examples of oncolytic virus include without limitation, adenovirus, reovirus, herpes simplex virus (HSV), Newcastle disease virus, poxvirus, myxoma virus, rhabdovirus, picornavirus, influenza virus, kievirus and parvovirus. In preferred embodiments, the tic virus is a ia virus (e.g. agen, Western Reserve, Wyeth strain), rhabdovirus (e.g. vesicular stomatitis virus (VSV)), or adenovirus (e.g. ONYX-015, Delta-24— RGD). In a particularly preferred embodiment, the oncolytic virus is an adenovirus. A preferred adenovirus is Delta—24—RGD. 24—RGD is a tumor—selective adenovirus serotype 5 strain sing a 24 base—pair on of the EIA region that encompasses the area responsible for binding Rb protein (nucleotides 923-946) corresponding to an eight amino-acids 120-127 in the encoded ElA protein (Fueyo I et al., ne, 1922-12 (2000)). DeltaRGD further comprises an insertion of the RGD-4C sequence (which binds strongly to OLVB3 and OLVBS integrins) into the H1 loop of the fiber knob protein (Pasqualini R. et al., Nat Biotechnol, 15:542— 546 (1997)). The ElA deletion increases the selectivity of the virus for cancer cells; the RGD— 4C sequence increases the ivity of the virus in s.

The term "providing" is used according to its ordinary meaning to indicate "to supply or furnish for use." In some embodiments, a protein is provided directly by administering the protein, while in other ments, the protein is effectively provided by administering a nucleic acid that encodes the protein.

The term "respond" generally means that a patient exhibits a complete or l response to the oncolytic therapy as defined in the Reesponse Evaluation Criteria in Solid Tumors T) criteria hauer et al., European Journal of , 45:228—247 , incorporated herein by reference). A complete response means a disappearance of all target lesions. A partial response means at least a 30% decrease in the sum of the longest diameter (LD) of target lesions, taking as a reference the baseline sum LD. In particular, a response generally refers to a complete or partial change in tumor size. Similarly, patients who fail to respond to oncolytic therapy are those that exhibit stable disease (neither sufficient shrinkage to qualify as a partial response nor ent increase to qualify as ssive disease) or progressive disease (at least a 20% increase in the sum of the LD of target lesions, taking as reference the smallest sum LD since the treatment started). The skilled clinician/radiologist will understand the appropriate methods for determining tumor measurements using e.g. ed tomography (CT) or magentic resonance imaging (MRI), in conjunction with clinical ment.

The term "therapeutic benefit" or "treatment" refers to ng that promotes or enhances the well-being of the subject with respect to the medical treatment of his/her condition, which includes treatment of pre—cancer, cancer, and hyperproliferative diseases. A list of nonexhaustive examples of this includes extension of the t‘s life by any period of time, decrease or delay in the neoplastic development of the disease, se in roliferation, reduction in tumor growth, delay of metastases, reduction in cancer cell or tumor cell proliferation rate, and a decrease in pain to the t that can be attributed to the subject's condition. It is not necessary that the cancer be cured to accomplish a meaningful treatment, all that is required is that the cancer be slowed to some degree or some condition associated with the cancer is ameliorated.

Other embodiments of the invention are discussed throughout this application. Any embodiment discussed with respect to one aspect of the invention applies to other aspects of the invention as well and Vice versa. Each embodiment described herein is understood to be embodiments of the invention that are applicable to all aspects of the invention. It is contemplated that any embodiment discussed herein can be implemented with respect to any method or composition of the invention, and Vice versa. Furthermore, compositions and kits of the invention can be used to achieve methods of the invention.

The use of the word "a" or "an" when used in conjunction with the term ising" in the claims and/or the specification may mean "one," but it is also consistent with the meaning of "one or more, H II at least one," and "one or more than one." Throughout this application, the term "about" is used to indicate that a value includes the standard deviation of error for the device or method being employed to determine the value.

The use of the term "or" in the claims is used to mean "and/or" unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive, although the disclosure supports a definition that refers to only alternatives and "and/or." As used in this cation and claim(s), the words ising" (and any form of comprising, such as "comprise" and "comprises"), "having" (and any form of having, such as "have" and "has"), "including" (and any form of including, such as "includes" and "include") or "containing" (and any form of containing, such as ins" and in") are inclusive or nded and do not exclude additional, unrecited ts or method steps.

Other objects, features and advantages of the present invention will become apparent from the ing detailed description. It should be understood, r, that the detailed description and the specific examples, while indicating specific embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.

A common feature to both primary and metastatic tumors to the brain is a lack of immunosurveillance with these . The normal antigen presenting cells within the brain, known as microglia, are frequently found to be "turned off" and therefore have a fiinctional lack of antigen presentation activities to the immune system. This lack of immunosurveillance is thought to be one reason why these tumors are so difficult to treat.

Data from a phase-1 al trial for treating malignant glioma with an oncolytic adenovirus (DeltaRGD) that replicates within primary gliomas showed unexpectedly that an immune response was raised not only to adenoviral antigens but also cancer related antigens.

Briefly, it has been discovered that these tumor associated antigens are released (or d) after the infection of Delta-24 in glioma cell lines. In addition, this phase-l al study unexpectedly showed massive recruitment of a large population of cytotoxic T-cell infiltrates WO 12942 with large regions of treatment d necrosis. This finding suggests a s of not only active oncolysis with the Delta—24-RGD agent but also cell mediated cytotoxicity to the tumor cells. Without being bound by theory it is believed that, in patients for whom treatment with Virus is sful, an initial immune response to the oncolytic virus is generated followed by a shift in immunity towards cancer related antigens.

The t inventors have discovered that a predominant expression of Th1 matory) cytokines correlates well with a positive outcome in patients undergoing oncolytic Virus therapy; accordingly the extent of Th1 polarization in these patients can be used to predict the outcome of oncolytic virus therapy in patients with cancers such as gliomas.

Without being bound by theory, it is proposed that patients having a predominantly Th1 cytokine profile are primed to mount a cell-mediated antitumor immnunoresponse to oncolytic viruses. In particular, the present inventors have surprisingly discovered that glioma patients with measurable ses to the oncolytic adenovirus DeltaRGD in a positive clinical e have high levels of Th1 cytokines IL-12p70, IL-2 and IFN- 7 whereas responses of Th2 humoral antibodies predicts that these patients will not respond to the virus. In the Phase I clinical study, patients that had a complete response or a high level of response had high levels of Th1 cytokine interleukin-12p70 eratively (serum levels were highly elevated ed to the rest of the population within the phase I trial) and this level increased after injection of the virus. The three patients who had a complete response to the virus also had serum levels of IL-12p70, which were highly elevated compared to the rest of the population within this phase I trial. The use of Th1 biomarkers for predicting the response of a patient with cancer to oncolytic virus therapy is provided.

One t in the clinical trial that exhibited low baseline levels of 70 (i.e. prior to treatment with DeltaRGD) was administered IFN—y two months after the virus was administered. IFN—g stimulated a Th1 immune response in the patient (indicated by an increase in IL—12 production) and corresponded with a very good response to the virus. Thus, methods for treating cancer comprising administration of agents which stimulate or boost the Th1 response and/or suppress regulatory T cells in combination with an oncolytic virus to cancer ts are also provided.

Malignant gliomas constitute the majority of primary cerebral malignant neoplasms.

These deadly tumors invariably recur after conventional therapy and the median al time of patients with glioblastoma (GBM) the most common form of malignant high-grade gliomas is 14 months. In addition to the difficulties to drug delivery imposed by the blood-brain—barrier, conventional chemotherapy and small-molecule approaches have been unable to significantly improve the prognosis of these patients. Oncolytic viruses such as Delta—24-RGD have emerged as a promising alternative to tional therapies for the ent of these tumors. r, it is currently not possible to predict whether a patient will respond favorably to such treatment.

Although emerging evidence suggests immune response during virotherapy is involved in anti— tumor ty, the role that isting immune conditions play during oncolytic virus treatment and its ce in clinical outcome is currently unknown. The present inventors have discovered that treatment with oncolytic s such as DeltaRGD induces autophagic cell death leading to asmic reticulum stress with antigen processing and epitope presentation in glioma-infected cells. The present inventors have also surprisingly discovered that patients who are good responders to oncolytic virus therapy exhibit a skew to the T-cell effector response rather than a humoral skewed response. Patients who are good responders to oncolytic virus therapy exhibit a specific Th1 biomarker profile. These biomarkers are of value in the prognosis and treatment of brain and other cancers including determining the optimum group of patients with cancers such as malignant gliomas that are likely to have positive clinical outcomes when treated with oncolytic viruses and thus significantly se their survival and determining whether a patient is a good ate for an additional treatment modality. It is expected that the present ker profiles are of relevance to the vast ty of solid tumors for which there is currently a dramatic paucity of biomarkers.

Biomarkers that may be measured or detected include, without limitation, proteins (e.g. cytokines, antibodies), cells (e. g. lymphocytes), nucleic acid, and metabolites. ns that can be used as biomarkers of the hood for response to an tic virus therapy include, but are not limited to cytokines such as lymphokines, monokines, growth factors and traditional polypeptide es. Included among the nes are growth hormones such as human growth hormone, N—methionyl human growth hormone, and bovine growth hormone,; parathyroid hormone; throxine; n; proinsulin; relaxin; prorelaxin; rotein hormones such as follicle ating hormone (FSH), thyroid stimulating hormone (TSH), and luteinizing hormone (LH); hepatic growth factor; prostaglandin; fibroblast growth factor; prolactin; placental en; OB protein; tumor necrosis factor-alpha and —beta; mullerian-inhibiting substance; mouse tropin-associated peptide; inhibin; activin; vascular endothelial growth factor; integrin; thrombopoietin (TPO); nerve growth factors such as NGF-beta; et-growth factor; transforming growth factors (TGFs) such as TGF-alpha and TGF-beta; insulin-like growth factor-1 and —II; erythropoietin (EPO); osteoinductive factors; erons such as interferon-alpha, —beta and —gamma; colony stimulating factors (CSFs) such as macrophage-CSF (M-CSF); granuloctye-macrophage-CSF (GM-CSF); and granulocyte—CSF (G—CSF); interleukins (ILs) such as IL-1, lL—lalpha, IL-2, 11—3, IL-4, IL-5, IL—6, IL-7, IL-8, IL—9, IL-10, IL—11, IL-12, IL—13, IL-l4, lL-15, IL-l6, IL-l7, 11-18, IL-l9, IL—20, IL-24, G-CSF, GM-CSF, EPO, kit-ligand or FLT-3. Other antigens can be measured, for example CMV antigens, EGFRVHI or lLl3R. In a further aspect, markers can be measured or detected that are indicative for blood vessel formation in general and for enesis and/or vasculogenesis of tumors in particular fibronectin, fibrinogen and acidic in 3 and colligin 2.

Nucleic acids that can be measured or detected include those mRNAs encoding the proteins described above, as well as various non-coding nucleic acid such as miRNAs. A number of nucleic acid arrays are commercially available for mRNA and miRNA.

Antibodies that can be measured or detected as kers of the likelihood for responding to oncolytic virus therapy include, but are not limited to, antibodies against tumor ated antigens such as BRAF (v-raf murine sarcoma Viral oncogene homolog Bl), CABYR (calcium binding tyrosine-(Y)-phosphorylation regulated), CRISP3 (cysteine-rich secretory n 3), CSAG2 (CSAG family, member 2), CTAG2 (cancer/testis n 2), DHFR (dihydrofolate reductase), FTHLl7 (ferritin, heavy polypeptide l; testis-specific expression), GAGEl (G antigen 1), LDHC (lactate dehydrogenase C), MAGEAl (melanoma antigen family A, 1), MAGEA3 (melanoma antigen family A,3), MAGEA4 (melanoma antigen family A, 4), MAGEB6 (melanoma antigen family B, 6), MAPKl en—activated protein kinase 1), MICA (MHC Class I polypeptide—related sequence A), MUCl (mucin 1, cell surface associated), NLRP4 (NLR family, pyrin domain ning 4), NY-ES—Ol (New York oesophageal squamos cell carcinoma 1), P53, PBK (PDZ binding kinase), PRAME (preferentially expressed n in melanoma), SOX2 (sex ining region Y-box 2), SPANXAl (sperm protein associated with the s, X—linked, family member Al), SSX2 (synovial sarcoma, X breakpoint 2), SSX4 (synovial sarcoma, X breakpoint 4), SSX5 (synovial sarcoma, X breakpoint 5), TSGAlO (testis specific, 10), TSSK6 (testis-specific serine kinase 6), TULP2 (tubby like protein), XAGE2 (X antigen family, member 2), and ZNF165 (zinc finger protein 165). It should be understood that antibody biomarkers of the invention are not limited and extend to antibodies against any tumor associated antigen. In a particular aspect, antibodies against cancer/testis antigens are used as biomarkers of the invention. Antibodies against tumor associated ns that have been identified as occuring in patients with brain cancers such as s are preferable for use as biomarkers of the invention which include but are not limited to: AIM2 (absent in melanoma 2), BMIl (BMIl polycomb ring finger oncogene), COX-2 (cyclooxygenase—2), TRP-l (tyrosine related protein 2) TRP-2 (tyrosine related protein 2), GPlOO (glycoprotein 100), I (epidermal growth factor receptor variant III), EZH2 (enhancer of zeste homolog 2), LICAM (human L1 cell adhesion molecule), Livin, LivinB, MRP-3 drug resistance protein 3), Nestin, OLIG2 (oligodendrocyte transcription factor), SOX2 (SRY-related HMG-box 2), ARTl (antigen recognized by T cells 1), ART4 (antigen recognized by T cells 4), SARTl (squamous cell carcinoma antigen recognized by T cells 1), SART2, SART3, B-cyclin, b-catenin, Glil (glioma-associated oncogene homlog 1), Cav-l (caveolin—l), cathepsin B, CD74 er of Differentiation 74), erin elial calcium-dependent adhesion), EphA2/Eck (EPH receptor A2/epithelia1 kinase), Fra-l/Fosl l (fos-related antigen 1), GAGE—1 (G antigen 1), Ganglioside/GD2, GnT-V, B1,6-N (acetylglucosaminyltransferase-V), Her2/neu (human epidermal growth factor receptor 2), Ki67 (nuclear proliferation-associated antigen of antibody Ki67), Ku70/80 (human Ku heterodimer proteins subunits), a2 (interleukin-13 receptor WO 12942 subunit alpha-2), MAGE-A (melanoma-associated antigen 1), MAGE-A3 (melanoma-associated antigen 3), NY-ESO-l (New York oesophageal squamos cell carcinoma 1), MART-l (melanoma antigen recognized by T cells), PROXl ero homeobox protein 1), PSCA (prostate stem cell antigen), SOX10 (SRY-related HMG-box 10), SOX11, in, UPAR (urokinase—type plasminogen activator receptor, and WT-l (Wilms’ tumor n 1).

Thus, in one embodiment, the expression level of antibodies against any combinatino ofat least 1, at least 2, at least 3, 4, 5, 6, 7, 8, 9,10,11,12,13,14,15,16,17,18,19, 20, 25, 30, , 40, 45, 50, 55, 60, 65, or at least 70 or more tumor associated antigens selected from the group consisting of: BRAF, CABYR, CRISP3, CSAG2, CTAG2, DHFR, FTHL17, GAGEl, LDHC, MAGEAl, MAGEA3, MAGEA4, , MAPKl, MICA, MUCl, NLRP4, NYESOl, P53, PBK, FRAME, SOX2, SPANXAl, SSX2, SSX4, SSXS, TSGAlO, TSSK6, TULP2, XAGE2, ZNF165, AIM2 , BMll, COX-2, TRP-l, TRP—2, GPlOO, II, EZH2, LICAM, Livin, LivinB, MRP-3, Nestin, OLIG2, SOX2, ARTl, ART4, SARTl, SART2, SART3, B-cyclin, b-catenin, Glil, Cav-l, cathepsin B, CD74, E-cadherin, EphA2/Eck, Fra—l/Fosl 1, Ganglioside/GD2, GnT—V,B1,6-N, Her2/neu, Ki67, Ku70/80, IL-13Ra2, MART-1, PROXl, PSCA, SOX10, SOX11, Survivin, UPAR, and WT-l may be measured in a test sample according to the methods described herein.

Thus, in one embodiment, the expression level of antibodies against any combination ofat least 1, 2, 3, 4, 5, 6, 7, 8, 9,10,11,12,13,14,15,16,17,18,19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45 or at least 46 glioma associated ns selected from the group consisting of: AIM2 , BMIl, COX-2, TRP—l, TRP-2, GP100, EGFRVIII, EZH2, LICAM, Livin, LivinB, MRP-3, Nestin, OLIG2, SOX2, ARTl, ART4, SARTl, SART2, SART3, B-cyclin, b-catenin, Glil, Cav—l, cathepsin B, CD74, E— cadherin, EphA2/Eck, Fra-l/Fosl 1, GAGE-1, oside/GDZ, GnT-V,Bl,6~N, Her2/neu , Ki67, Ku70/80, IL-13Ra2, MAGE-A, MAGE-A3, NY-ESO-l, , PROXl, PSCA, SOX10, SOX11, in, UPAR, and WT-l may be measured in a test sample according to the methods described herein.

In other embodiments, the expression level of antibodies against any ation of tumor associated ns as bed herein and the expression level of one or more Th1 biomarkers and optionally the expression level of one or more Th2 biomarkers may be measured in a test sample ing to the methods described .

Cells that can be measured or detected include CD4 T cells, CD8 T cells, regulatory T cells, microglia and the like.

CD4 T cells s the CD4 protein on their surface. Upon presentation of antigens by major histocompatibility complex (MHC) class II molecules, naive CD4 T cells mainly differentiate into Th1, Th2 or Thl7 (regulatory T cells) cells, each type secreting a different set of cytokines to facilitate a different type of immune response. For example, Th1 cells secrete IL- 2, IFN—y and TNF-B; Th2 cells secrete IL—4, IL-5, IL-6, IL-10 and IL-13; and Th17 cells secrete IL—17oc.

CD8 T cells (cytotoxic T cells or CTLs) express the CD8 glycoprotein at their surface and upon presentation of antigen associated with MHC class I molecules, y virally infected cells and tumor cells.

Regulatory T cells (also known as suppressor T cells or Thl7 cells) are CD4+CD25+FoxP3Jr and act to shut down T cell mediated-immunity toward the end of an immune reaction and suppress auto-reactive T cells that escape negative selection in the thymus.

Natural killer T (NKT) cells bridge the ve immune system with the innate immune system. Unlike tional T cells that recognize peptide antigens presented by MHC molecules, NKT cells recognize glyclolipid antigen presented by a molecule called CDld. Once activated, these cells can produce cytokines and release cytolytic/cell killing molecules. They are also able to recognize and eliminate some tumor cells and cells infected with viruses.

Microglia are glial cells that are the resident macrophages of the brain and spinal cord, and the primary mediator of active immune defense in the central nervous system (CNS).

Microglia constitute 20% of the total glial cell tion within the brain. Microglia are constantly scavenging the CNS for infective agents. The brain and spinal cord are considered "immune privileged" organs in that they are separated from the rest of the body by a series of endothelial cells known as the blood-brain barrier, which prevents most infections from reaching the vulnerable nervous tissue. In the case where the infectious agents are directed introduced to the brain or cross the blood-brain barrier, lial cells must react quickly to decrease inflammation and destroy the infectious agents before they damage the sensitive neural tissue.

Due to the unavailability of antibodies from the rest of the body (few antibodies are small enough to cross the blood brain barrier), microglia must be able to recognize foreign bodies, swallow them, and act as antigen-presenting cells ting T cells.

Isolation of Samples Samples (test samples) can be obtained from a patient prior to, concurrently with, or subsequent to treatment with an oncolytic virus from, without limitation, tissue (e.g. tumor biopsy), cerebrospinal fluid, blood, plasma, serum, lymph, and synovial fluid. Standard procedures that are known in the art for obtaining such samples are used. In a red embodiment, the sample is a serum sample and the level of one or more biomarkers described herein is determined by enzyme linked immunosorbent assay ).

[O97] Procedures used to biopsy a tumor e, but are not limited to, stereotactic biopsy, which can be done by precise introduction of a metal probe into the brain tumor, cutting a small piece of the brain tumor, and removing it so that it can be examined. For example, the patient is transported to the MRI or CAT scan suite, and a frame is attached to the scalp under local anesthesia. The "pins" of the frame attach to the skull for rigid fixation (frame will not and can not move from that point forward until completion of the biopsy). The scan (MRI or CT) is obtained. The neurosurgeon examines the scan and determines the safest trajectory or path to the target. This means avoiding critical structures. The spatial co-ordinates of the target are determined, and the optimal path is elected. The biopsy is d out under l anesthesia.

A small incision is created over the entry point, and a small hole is drilled through the skull. The "dura" is perforated, and the biopsy probe is introduced slowly to the target. The biopsy en is awn and placed in fluids or vative to assess biomarkers.

Once samples are obtained, the immune status of a subject is determined by measuring or detecting or analyzing various biomarkers to e data for the determination of the subject’s immune status (e.g. the degree of Th1 polarization) in order to determine 6. g. the susceptibility of a patient to oncolytic Virus therapy, or the need for co-administration of an agent that stimulates a Th1 immune response with the oncolytic virus therapy.

In certain embodiments, the ction or response of the tumor to the therapy is d to the immunologic status of the tumor or surrounding tissue, the istence of anti- virus antibodies and/or the presence and degree of lymphopenia prior to treatment. It is envisaged that a patient having an immunologic status that ts immunosuppression may indicate that a tumor will be resistant to oncolytic virus therapy.

Assessement of biomarkers Assessment of expression levels of the markers discussed above may be direct, as in the use of immunohistochemistry (IHC) (including uantitative or quantitative IHC) or other antibody-based assays (Western blot, fluorescent immunoassay (FIA), fluorescence in situ hybridization (FISH), radioimmunoassay (RIA), radioimmunoprecipitation (RIP), enzyme-linked immunosorbent assay (ELISA), immunoassay, immunoradiometric assay, mmunoassay, chemiluminescent assay, bioluminescent assay, gel ophoresis), or indirectly by quantitating the transcripts for these genes (e. g. by in situ ization, nuclease protection, Northern blot, polymerase chain on (PCR) including reverse transcriptase PCR (RT-PCR)). Cells, for example, lymphocytes, can be analyzed using FACs technology or paraffin embedded tumor sections using antibodies. nt methodologies are discussed below.

Antibodies can be used in the present invention to characterize the protein t of target cells through techniques such as immunohistochemistry, ELISAs and Western blotting.

This may provide a screen e.g. for the presence or absence of a subject likely to respond favorably to oncolytic virus therapy and/or a need for co-administering an immune stimulating agent with an oncolytic virus.

Immunohistochemistry is typically performed on a sample of tissue from a biopsy.

The sample can be examined fresh or frozen. The tissue sample is sliced extremely thin, so that it is approximately one cell thick, then the sample is fixed onto a glass slide. The cells in the sample have characteristic antigens on their cell es that can be used to help identify the c type of cell. Antibodies against these characteristic antigens are added to the sample on the slide and the antibodies bind wherever the antigens are present. Excess antibody is then washed away. The antibodies that remain bound to the cell have labels that either fluoresce or undergo a chemical on that makes them visible by microscope.

The use of antibodies in an ELISA assay is contemplated if the sample is a tissue lysate, blood, serum or cerebrospinal fluid. For example, antibodies against the n to be detected are immobilized onto a ed surface, preferably a surface exhibiting a protein affinity such as the wells of a polystyrene microtiter plate. After g to remove incompletely adsorbed material, it is desirable to bind or coat the assay plate wells with a non- specific n that is known to be antigenically neutral with regard to the test antisera such as bovine serum albumin (BSA), casein or solutions of powdered milk. This allows for blocking of nonspecific adsorption sites on the immobilizing surface and thus reduced the ound caused by non-specific g of antigen to the surface.

After binding of antibody to the well, coating with a non-reactive material to reduce background, and washing to remove unbound material, the immobilizing surface is contacted with the sample to be tested in conditions conducive to immune complex (antigen/antibody) formation.

Following formation of specific immunocomplexes between the test sample and the bound antibody, and subsequent washing, the occurrence and even amount of immunocomplex formation may be determined by subjecting the same to a second dy having specificity for an antigen that differs from that recognized by the first antibody. Appropriate conditions preferably include diluting the sample with diluents such as BSA, bovine gamma in (BGG) and phosphate buffered saline (PBS)/Tween®. These added agents also tend to assist in reduction of nonspecific background. The layered antisera is then allowed to incubate for from about 2 to about 4 hours, at temperatures preferably on the order of about 25° to 270 C.

Following incubation, the antisera-contacted surface is washed so as to remove non- immunocomplexed material. A preferred washing procedure includes washing with a solution such as PBS/Tween®, or borate buffer.

To provide a detecting means, the second antibody will preferably have an associated enzyme, or detectable moiety, that can be detected, e. g., will generate a color development upon incubating with an appropriate chromogenic substrate. Thus, for example, one will desire to contact and incubate the second antibody—bound surface with a urease or dase-conjugated anti-human IgG for a period of time and under ions which favor the development of immunocomplex formation (e. g. incubation for 2 hours at room temperature in a PBS—containing solution such as een®).

After incubation with a second detectable dy, and subsequent to g to remove unbound material, the amount of label is quantified by incubation with a chromogenic substrate such as urea and bromocresol purple or 2,2’—azino—di—(3-ethyl-benzthiazoline)-6— sulfonic acid (ABTS) and H202, in the case of dase as the enzyme label. Quantitation is then achieved by measuring the degree of color tion, e.g. using a Visible spectrum spectrophotometer.

The preceding format may be altered by first binding the sample to the assay plate, then contacting the sample with a primary antibody, followed by detecting bound primary antibody using a labeled second antibody with specificity for the primary antibody.

Antibodies can also find use in immunoblots or Western blot analysis. The antibodies may be used as high affinity primary reagents for the identification of proteins immobilized onto a solid support matrix, such as nitrocellulose, nylon or combinations thereof. In conjunction with precipitation, followed by gel electrophoresis, these may be used as a single step reagent for use in ing antigens against which secondary ts used in the detection of the antigen cause an adverse background. Immunologically-based detection s for use in conjunction with Western blotting include enzymatically—, radiolabel-, or fluroescently-tagged secondary dies against the antigen of interest are considered to be of particular use in this regard.

Aspects of the methods described herein are related to one or more of (i) stimulating a cellular immune response in a subject in ation with administration of an oncolytic virus (e. g. adenovirus), (ii) antagonizing immune suppression in combination with administration of an oncolytic virus (e.g. adenovirus), or (iii) stimulating a cellular immune response in a subject and antagonizing immune suppression in a subject combination with administration of an oncolytic virus (e. g. adenovirus).

A. Antagonism of immune suppression Immunosuppression involves an act that reduces the tion or efficacy of the immune system. Some portions of the immune system itself have immuno-suppressive effects on other parts of the immune system, and immunosuppression may occur as an affect of certain diseases or conditions. Disease related immunosuppression can occur in, for e, malnutrition, aging, many types of cancer (such as , leukemia, lymphoma, multiple myeloma), and certain chronic infections such as acquired immunodeficiency syndrome (AIDS).

The unwanted effect in disease related immunosuppression is immunodeficiency that results in increased susceptibility to the growth of hyperproliferative cells.

In n aspects, the therapeutic methods described herein may reduce immunosuppression in a subject and e the oncolytic effect of viral therapies. In n aspects, the methods may reduce the regulatory T cell activity in the subject. ng immunoregulatory T cell activity may be achieved by administering an agent (e. g. an alkylating agent) to the individual that depletes or inactivates regulatory T cells in the individual.

Reducing immunoregulatory T cell activity also may be achieved by using at least one antibody that binds to the immunoregulatory T cells. Such antibody may be selected from, but not limited to anti-CD4, anti-CD25, anti-neuropilin, and/or anti-CTLA4. Immunoregulatory T cell activity may be reduced in the individual before, during, or after administering an oncolytic virus. The term "depleting or inactivating in vivo immunoregulatory T cells" as used herein refers to a reduction in the number or functional capability of immunoregulatory T cells that suppress the host umor immune response. Antagonism of immune suppression can be ed through a reduction of immunoregulatory T cells (i.e., depletion) or inactivation of anti-tumor immune suppression function of the immunoregulatory T cells. The ultimate result of such treatment is to reduce immunoregulatory T cell activity in the recipient of the treatment.

Depleting or inactivating immunoregulatory T cells may be achieved by administering a pharmaceutical agent such as an antibody specific for the CD4 antigen, the alpha chain subunit of the IL—2 receptor (i.e. CD25), and the like and as bed herein. Also, an antibody to gamma delta immunoregulatory T cells can be used to deplete such cells and stimulate anti-tumor ty. Seo et al., J. l. (1999) 1632242-249. Anti—CD40 ligand, also may be used to deplete or inactivate immunoregulatory T cells.

Partial antibody ucts such as CTLA4Ig, a fusion protein of CTLA-4 and Fe of immunoglobulin (Ig) heavy chain, can be used to inhibit the essential co—stimulatory signal for full T cell activation via blocking the ction between CD28 and B7 molecules. CTLA4Ig may be administered as a pharmaceutical to render regulatory T cells nonresponsive (i.e. inactivation). See Park et al. Pharm Res. (2003) 1239-48. An lL—2 fusion to pseudomonas exotoxin (OnTac) is yet another agent for depleting or inactivating regulatory T cells.

In another approach, agents may be administered that prevent the induction of CD8+ cytolytic T-lymphocyte (CTL) tumor anergy. Agents that agonize CD137, such as agonistic antibodies, may be used to restore the tumor cytolytic function of established anergic CTLs upon reencountering their cognate antigen. See Wilcox et al., Blood (2004) 103:177-184. This approach can be used to break T-cell tolerance to tumor antigens.

Agents that agonize glucocorticoid-induced tumor necrosis factor receptor (GITR) ligand on CD4/CD25+ immunoregulatory T cells reverses the suppressive action of these cells.

GITR ligand agonists are bed in Tone et al., PNAS (2003) 100:15059-15064; Stephens et al. 2004 and Shimizu et al. 2002. dies to neurophilin (e.g. Bruder et al. 2004) and antibodies to CTLA-4 (e.g.

Leach et a1. 1996) also can be administered in vivo to deplete immunoregulatory T cells or reduce their activity.

Methods of removing, depleting or inactivating immunoregulatory T cells may be used even if the methods are not limited solely to such cells. Effort to remove, e or inactivate immunoregulatory T cells may be med multiple times during a given period of treatment. Also, different methods may be used together (e.g., ex Vivo cell removal and in vivo depletion or inactivation). The amount of anti-T cell antibody administered for ion or inactivation may be similar to the amount used in the transplantation field. See, e.g., Meiser et al., Transplantation. (1994) 27; 58(4): 419-23.

Immunoregulatory T cells may be removed, depleted or inactivated before, during and/or after stration of an oncolytic virus. lmmunoregulatory T cells are preferably removed, depleted or inactivated before administering the oncolytic virus.

B. Stimulation of the immune system The terms "enhance the cellular immune system" and "stimulate the cellular immune system" (and different tenses of these terms) refer to the ability of an agent to stimulate the generation of antigen—specific tic activity (the activity of immune cells, particularly xic hocytes) and/or NK cell activity, improve the cellular immune response to antigens (through the activity of at least cytotoxic hocytes), improve immune protection (by at least restoring the activity of cytotoxic T-lymphocytes and/or NK cells and enhancing cytokine production), restore immune protection (by at least restoring or stimulating the activity of cytotoxic T-lymphocytes and/0r NK cell activity and enhancing cytokine tion) or generate pro—inflammatory (Th1) cytokines.

Agents which enhance the cellular immune system (or produce a Th1 phenotype) include cytokines (preferably recombinant) representative examples of which are GM—CSF, IL-2, IL-12, IL-18 and interferon-y. These cytokines can be stered prior to, during, or subsequent to oncolytic virus therapy in order to improve the patient’s response to the virus.

Recombinant cytokines are commercially able and are stered according to their recommended dosages. Other agents which produce a Th1 phenotype include agents which stimulate the production of lL—l2p70 and/or other Th1 cytokines including t limitation lenalidomide (Revlimid) and pomalidomide.

Other agents which produce a Th1 phenotype include alkylating agents entative es of which are Temozolomide, cyclophosphamide, lomustine (CCNU), bis— chloroethylnitrosourea (BCNU), melphalan hydrochloride, busulfan e-1,4-diyl dimethanesulfonate), mechlorethamine (nitrogen mustard), chlorambucil, ifosfamide, streptozocin, dacarbazine (DTIC), thiotepa, altretamine (hexamethylmelamine), cisplatin, carboplatin, and oxalaplatin. The use of these alkylating agents for treating s types of cancer is well ished and they may be used at their recommended s in order produce a Th1 phenotype.

Other agents which produce a Th1 phenotype include adjuvants, nonlimiting examples of which are monophosphoryl lipid A (MPL®), QS-21 (plant extract comprising soluble triterpene glucoside compounds) or other saponin, oligodeoxynucleotides comprising or consisting of CpG, and ribosomal protein extract (RPE).

Other agents which produce a Th1 phenotype include CD137 agonists such as BMS- 663513, CD40 agonists, such as CP-870,893, 0X40 (CD134) agonists and CD27 agonists such as CDX-1127.

Other agents which e a Thl phenotype include inhbitors of JAK—2, JAK-3, STAT-3, or STAT-5.

Other agents which produce a Th1 ype include CTLA-4 antagonists (e.g. lpilimumab or Tremelimumab), PD-l/PD—Ll — receptor antagonists (e.g. MDX-1106, MK-3475, AMP-224, Pidilizumab, or MDX-llOS); antibodies that specifically bind to B7-H3 such as MGA271, and indoleamine-2,3-dioxygenase (IDO) inhibitors such as D-l-methyl-tryptophan (Lunate).

In a further embodiment, the methods for treating cancer may include adoptive transfer of immune cells to enhance anti-tumor immunity. As used herein "adoptive transfer" refers to the administration of immune cells, from another individual or from the same individual. These are preferably T cells, which may be activated ex vivo to enhance their ability to function in supporting an anti-tumor immune se. Adoptively erred immune cells may be activated ex Vivo by any of a variety of well known agents including, for example, exposure to lL—2 and/or to anti-CD3 antibodies. Ex vivo activation also may include exposure to a cancer cell vaccine. Such cancer cell vaccine may tute live (but plicating), or killed cancer cells from the individual to be treated or from r cancer entirely. The vaccine also may be a cancer cell extract or purified vaccine preparation d from cancer cells.

Cancer cell vaccines are well known in the art and may be prepared in accordance with well known methods.

Ill. Antibodies An antagonist of an immune suppressor or a stimulator of the immune system can be an dy. The term "antibody" as used herein includes immunoglobulins, which are the product of B cells and variants thereof as well as the T cell receptor (TCR), which is the product of T cells, and variants thereof. An immunoglobulin is a protein comprising one or more polypeptides substantially encoded by the immunoglobulin kappa and lambda, alpha, gamma, delta, epsilon and mu constant region genes, as well as myriad immunoglobulin variable region genes. A typical immunoglobulin structural unit is known to comprise a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one "light" (about 25 kD) and one "heavy" chain (about 50-70 kD). The N-terminus of each chain defines a variable region of about 100 to 110 or more amino acids primarily responsible for antigen ition.

The terms variable light chain (VL) and variable heavy chain (VH) refer to these light and heavy chains respectively.

Recombinant dies may be tional full length antibodies, antibody fragments known from proteolytic digestion, unique antibody fragments such as Fv or single chain Fv (scFv), domain deleted antibodies, and the like. An Fv antibody is about 50 Kd in size WO 12942 and comprises the variable regions of the light and heavy chain. A single chain FV ") polypeptide is a covalently linked VH::VL heterodimer which may be expressed from a nucleic acid including VH- and VL—encoding sequences either joined directly or joined by a peptide- encoding linker. See , et al. (1988) Proc. Nat. Acad. Sci. USA, 85:5879-5883. A number of structures for converting the naturally aggregated, but chemically separated light and heavy polypeptide chains from an antibody V region into an scFV molecule which will fold into a three dimensional structure ntially similar to the structure of an antigen-binding site. See, e.g.

U.S. Patents 5,091,513; 5,132,405; and 4,956,778.

An antibody may be a non-human antibody, a human antibody, a humanized antibody or a chimeric antibody, the latter sing human and non-human antibody sequence. As is known in the art, chimeric antibody is prepared by exchanging a non-human constant region (heavy chain, light chain or both) with a human constant region antibody. See e.g. U.S. Patent 4,816,567. Methods of making humanized antibodies from non-human antibodies such as from murine antibodies are also well known (see, e.g., U.S. Patent 5,565,332). 1V. Oncolytic Virus Oncolytic viruses that can be administered ing to the methods of the invention include, t limitation, iruses (e.g. Delta-24, DeltaRGD, ICOVIR-S, —7, Onyx-015, ColoAdl, H101, AD5/3-D24-GMCSF), reoviruses, herpes simplex virus (HSV; OncoVEX GMCSF), Newcastle Disease Virus, s viruses, iruses (e.g. influenza Viruses), poxviruses (e.g. vaccinia Virus including Copenhagen, Western Reserve, Wyeth strains), myxoma viruses, rhabdoviruses (e..g vesicular stomatitis virus (VSV)), picomaviruses (e.g. Seneca Valley Virus; SVV-OOl), coxsackievirus and parvovirus.

In preferred embodiments, the oncolytic Virus is an adenovirus including members of any of the 57 human serotypes f (HAdV-l to 57). In one aspect, the adenovirus is an Ad5 serotype. In other aspects, the adenovirus is hybrid serotype which may or may not comprise an Ad5 component. Non-limiting examples of adenoviruses that may be administered according to the present methods include Delta-24, DeltaRGD, ICOVIR—S, -7, ONYX-015, ColoAdl, H101 and AD5/3-D24-GMCSF. Onyx-015 is a hybrid of virus serotype Ad2 and Ad5 with deletions in the BIB—55K and E3B regions to e cancer selectivity. H101 is a modified version of Onyx-015. ICOVIR-S and ICOVIR-7 se an Rb-binding site deletion of EIA and a replacement of the EIA promoter by an E2F promoter. ColoAdl is a chimeric Addllp/Ad3 pe. ADS/3-D24-GMCSF (CGTG—102) is a serotype 5/3 capsid-modified adenovirus ng GM-CSF (the Ad5 capsid protein knob is replaced with a knob domain from serotype 3).

In one particularly preferred embodiment, the oncolytic virus is Delta-24 or Delta—24— RGD. 24 is described in US. Patent Application Publication Nos. 20030138405, and 20060147420, each of which are incorporated herein by reference. The Delta-24 adenovirus is derived from adenovirus type 5 (Ad-5) and contains a 24-base-pair deletion within the CR2 portion of the ElA gene. DeltaRGD r comprises an insertion of the RGD—4C sequence (which binds strongly to och3 and 0Lv[35 integrins) into the H1 loop of the fiber knob protein (Pasqualini R. et al., Nat Biotechnol, 152542-546 (1997)).

Significant antitumor effects of Delta-24 have been shown in cell culture systems and in malignant glioma xenograft . Currently delta-24 is showing anti-tumor efficacy in clinical trials. Conditionally replicating adenoviruses ), such as Delta-24, have several properties that make them candidates for use as biotherapeutic agents. One such property is the ability to replicate in a permissive cell or tissue, which amplifies the original input dose of the oncolytic virus and spreads the virus to adjacent tumor cells providing a direct antitumor .

The in Vitro and in vivo oncolytic effects of delta-24 adenovirus have been demonstrated. Generally, adenovirus is a 36 kb, linear, double-stranded DNA virus (Grunhaus and Horwitz, 1992). Adenoviral infection of host cells results in adenoviral DNA being maintained episomally, which s the ial xicity associated with ating vectors. Also, adenoviruses are structurally , and no genome rearrangement has been detected after extensive amplification. Adenovirus can infect virtually most epithelial cells regardless of their cell cycle stage. So far, adenoviral infection appears to be linked only to mild disease such as acute respiratory disease in humans.

Several factors favor the use of oncolytic adenoviruses for the ent of brain tumors. First, gliomas are typically zed, and therefore an efficient local approach should be enough to cure the disease. Second, gliomas harbor several populations of cells expressing different genetic abnormalities (Sidransky et al., 1992; Collins and James, 1993; Fumari et al., 1995; Kyritsis et al., 1996). Thus, the spectrum of tumors sensitive to the transfer of a single gene to cancer cells may be limited. Third, ation competent adenoviruses can infect and destroy cancer cells that are arrested in G0. Since gliomas invariably include non—cycling cells, this property is important. Finally, the pl6—Rb pathway is abnormal in the majority of gliomas (Hamel et al., 1993; Henson et al., 1994; en et al., 1994; Jen et al., 1994; Schmidt et al., 1994; Costello et al., 1996; Fueyo et al., 1996b; Kyritsis et al., 1996; Ueki et al., 1996; Costello et al., 1997), thus making the delta—24 strategy appropriate for most of these tumors. Although the loss of the retinoblastoma tumor suppressor gene function has been associated with the causes of various types of tumors and is not limited to treatment of gliomas.

If an adenovirus has been d so that it is unable to replicate or is ionally replicative (replication-competent under certain conditions), a helper cell may be required for viral ation. When required, helper cell lines may be derived from human cells such as human embryonic kidney cells, muscle cells, hematopoietic cells or other human embryonic mesenchymal or epithelial cells. Alternatively, the helper cells may be derived from the cells of other mammalian species that are permissive for human adenovirus. Such cells e, for example Vero cells or other monkey nic mesenchymal or epithelial cells. In n s a helper cell line is 293. Various methods of culturing host and helper cells may be found in the art, for example Racher et al., 1995.

In certain aspects, the adenovirus is lly replication-competent in cells with a mutant Rb pathway. After transfection, adenoviral plaques are isolated from the agarose- overlaid cells and the viral particles are expanded for analysis. For detailed protocols the skilled artisan is referred to Graham and Prevac, 1991.

Alternative logies for the generation of adenovirus vectors include utilization of the bacterial artificial chromosome (BAC) system, in vivo bacterial recombination in a recA+bacterial strain utilizing two plasmids ning complementary adenoviral sequences, and the yeast artificial chromosome (YAC) system (PCT publications 95/27071 and 96/33280, which are incorporated herein by reference).

Adenovirus is easy to grow and manipulate and exhibits broad host range in Vitro and in vivo. This group of viruses can be obtained in high titers (e.g., 109-10n plaque forming units (pfu) per ml), and they are highly ive. The life cycle of adenovirus does not require integration into the host cell genome.

Modifications of oncolytic adenovirus described herein may be made to improve the ability of the oncolytic adenovirus to treat cancer. Such modifications of an oncolytic adenovirus have been described by Jiang et a1. (Curr Gene Ther. 2009 Oct 9(5):422-427), see also US. Patent ation No. 20060147420, each of which are incorporated herein by reference.

The absence or the presence of low levels of the coxsackievirus and adenovirus receptor (CAR) on l tumor types can limit the efficacy of the oncolytic adenovirus.

Various peptide motifs may be added to the fiber knob, for instance an RGD motif (RGD sequences mimic the normal ligands of cell surface integrins), Tat motif, polylysine motif, NGR motif, CTT motif, CNGRL motif, CPRECES motif or a strept-tag motif ahti and Rajotte, 2000). A motif can be inserted into the HI loop of the adenovirus fiber protein. Modifying the capsid allows CAR independent target cell infection. This allows higher ation, more efficient infection, and increased lysis of tumor cells i et al., 2001, orated herein by reference). Peptide sequences that bind c human glioma receptors such as EGFR or uPR may also be added. Specific receptors found exclusively or preferentially on the surface of cancer cells may be used as a target for adenoviral binding and infection, such as EGFRVIH.

Oncolytic viruses according to the invention may be administered locally or ically. For example, without limitation, oncolytic viruses ing to the invention can be administered intravascularly (intraarterially or intravenously), intratumorally, intramuscularly, intradermally, intraperitoneally, subcutaneously, orally, parenterally, intranasally, intratracheally, aneously, intraspinally, ocularly, or ranially.

Oncolytic viruses according to the invention may be administered in a single administration or multiple administrations. The virus may be administered at dosage of l X 105 plaque forming units (PFU), 5 x 105 PFU, at least 1 x 106 PFU, 5 x 106 or about 5 x 106 PFU, 1 x 107, at least 1 X 107 PFU, l X 108 or about 1 X 108 PFU, at least 1 X 108 PFU, about or at least 5 X 108 PFU, 1 x 109 or at least 1 x 109 PFU, 5 x 109 or at least 5 x 109 PFU, 1 x 1010 PFU or at least 1X1010 PFU, 5 X 1010 or at least 5 X 1010 PFU, 1 X 1011 or at least 1 X10", 1 X 1012 or at least 1 X 1012, 1 X 1013 or at least 1 X 1013. For example, the virus may be administered at a dosage of between about 107-1013, between about 108-1013, between about 109-1012, or between about 108- 1012.

Oncolytic viruses according to the invention may also be administered in a cellular carrier. In this respect, neuronal and mesenchymal stem cells have high migratory ial yet remain confined to tumor . A subpopulation of adult mesenchymal cells (bone marrow derived tumor infiltrating cells or BM-TICS) has been shown, following injection into gliomas, to infiltrate the entire tumor. Thus, oncolytic Viruses according to the invention can be administered in a virus-producing neuronal or mesenchymal stem cell (e.g. ) carrier (6.g. by injection of the carrier cell into the tumor).

A. Combination Therapies Additional therapies may be combined with any of the methods of the invention heretofore described in order to se the killing of cancer cells, the inhibition of cancer cell growth, the inhibition of angiogenesis or otherwise improve the reverse or ion of malignant phenotype of tumor cells. These compositions would be provided in a combined amount effective to kill or inhibit proliferation of the cell. This process may involve contacting the cells with the expression construct and the agent(s) or (s) at the same time. This may be achieved by ting the cell with a single composition or pharmacological formulation that includes both agents, or by contacting the cell with two distinct compositions or formulations, at the same time, wherein one composition includes the oncolytic virus and the other includes a second agent therapy.

Alternatively, the treatment may precede or follow the other agent or treatment by intervals ranging from minutes to weeks. In ments where the agents are applied separately to the cell, one would generally ensure that a significant period of time did not expire between each delivery, such that the agents would still be able to exert an advantageously ed effect on the cell. In such instances, it is contemplated that one would contact the cell with both modalities within about 12-24 hours of each other and, more preferably, within about 6—12 hours of each other, with a delay time of only about 12 hours being most red. In some situations, it may be desirable to extend the time period for treatment significantly, however, where several days (2, 3, 4, 5, 6 or 7) to several weeks (1, 2, 3, 4, 5, 6, 7 or 8) to several months (1, 2, 3, 4, 5, 6, 7 or 8) lapse between the respective administrations.

It also is conceivable that more than one administration of either agent will be desired. Various combinations may be ed, e.g. where one or more tic virus treatment is administered before the administration of a second agent; or the second agent may be administered prior to oncolytic virus administration. Successive administration can include one or more administration of the oncolytic Virus therapy or second agent. Again, to achieve cell killing, both agents are delivered to a cell in a combined amount effective to kill the cell. For example, the combination of Delta-24 or DeltaRGD and immune tor. ative Cancer Therapies In accordance with n embodiments of the present invention, methods for treating cancer are ed that can be used in conjunction with oncolytic virus y once a subject is identified as a responder or likely to respond to such therapy (e.g. deltaRGD therapy). Such therapies may be utilized when the assays of the present invention indicate that a subject is unlikely to d to treatment with a replication competent oncolytic virus such as adenovirus (e. g. deltaRGD). Alternatively, such therapies may be utilized in combination with replication competent oncolytic Virus such as adenovirus in the case that a subject is identified by the present s as unlikely to respond to treatment with only ation competent oncolytic virus.

Surgery Approximately 60% of persons with cancer will o y of some type, which includes preventative, diagnostic, staging, curative and palliative surgery. Curative surgery is a cancer treatment that may be used in conjunction with other therapies, such as the treatment of the present invention, chemotherapy, radiotherapy, hormonal y, gene therapy, immunotherapy and/or alternative therapies.

Curative surgery includes resection in which all or part of cancerous tissue is physically removed, d and/or destroyed. Tumor resection refers to physical removal of at least part of a tumor. In addition to tumor resection, treatment by surgery includes laser surgery, rgery, electrosurgery, and microscopically controlled y (Mohs’ surgery). It is further contemplated that the present invention may be used in ction with removal of superficial cancers, precancers, or incidental amounts of normal tissue.

In certain aspects, a therapy is administered by intratumoral injection prior to surgery or upon excision of a part of or all of cancerous cells, tissue or tumor. Treatment may also be accomplished by ion, direct injection or local application of these areas with an additional anti-cancer therapy. Such treatment may be repeated, for example, every 1, 2, 3, 4, 5, 6, or 7 days, or every 1, 2, 3, 4, and 5 weeks or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months.

These treatments may be of varying dosages. [01 56] Chemotherapy A wide variety of chemotherapeutic agents may be used in accordance with the t invention. The term "chemotherapy" refers to the use of drugs to treat cancer. A "chemotherapeutic agent" is used to connote a compound or composition that is administered in the treatment of cancer. These agents or drugs are categorized by their mode of activity within a cell, for example, whether and at what stage they affect the cell cycle. Alternatively, an agent may be terized based on its ability to directly cross—link DNA, to intercalate into DNA, or to induce chromosomal and mitotic aberrations by affecting nucleic acid synthesis. Most chemotherapeutic agents fall within the following categories: alkylating agents, antimetabolites, antitumor antibiotics, topoisomerase inhibitors, and mitotic inhibitors.

Alkylating agents direct interact with genomic DNA to prevent the cancer cell from proliferating. This category of drugs include agents that affect all phases of the cell cycle and are commonly used to treat chronic leukemia, non-Hodgkin’s lymphoma, Hodgkin’s disease, malignant melanoma, multiple myeloma, and particular cancers of the breast, lung, and ovary.

They include nitrogen mustards such as mechlorethamine (nitrogen mustard), chlorambucil, cyclophosphamide (Cytoxan®), ifosfamide and melphalan, nitrosoureas such as streptozocin, carmustine (BCNU) and ine, alkyl sulfonates such as busulfan, triazines such as dacarbzine (DTIC) and temozolomide ar®), nimines such as thiotepa and altretamine (hexamethylmelamine), and platinum drugs such as cisplatin, carboplatin, and oxalaplatin.

Antimetabolites disrupt DNA and RNA synthesis. Unlike ting agents, they specifically ce the cell cycle during S phase. They have been used to combat chronic leukemias, and tumors of the breast, ovary and gastrointestinal tract. Antimetabolites include 5- fluorouracil (S—FU), 6—mercaptopurine (6-MP), capecitabine (Xeloda®), cladribine, abine, bine (Ara-C®), floxuridine, fludarabine, gemcitabine (Gemzar®), hydroxyruea, methotrexate, pemetrexed, pentostatin and thioguanine.

Antitumor antibiotics have both antimicrobial and cytotoxic activity. These drugs also interfere with DNA by chemically inhibiting enzymes and mitosis or altering ar membranes. These agents work in all phases of the cell cycle and are used to treat a variety of cancers. Representative es include ubicin, doxorubicin (Adriamycin®), epirubicin, idarubicin, actinomycin-D, bleomycin and cin—C. Generally, these compounds are administered by bolus i.v. injections at doses ranging from 25-100 mg/kg Topoisomerase inhibitors interfere with topoisomerases, enzymes which help separate DNA strands so they can be copied and are used to treat certain leukemias, as well as lung, ovarian, gastrointestinal and other cancers and include topotecan, irinotecan, etoposide (VP-l6) and teniposide.

Mitotic tors, often plant alkaloids, work during M phase of the cell cycle and prevent mitosis or inhibit enzymes from producing proteins required for cell reproduction.

Representative examples include s such as paclitaxel (Taxol®) and docetaxel (Taxotere®), epothilones such as ilone (Ixempra®), vinca alkaloids such as Vinblastine (Velban®), vincristine vin®) and vinorelbine (Navelbine®), and Estramustine (Emcyt®).

Other chemotherapeutic agents include targeted therapies such as imatinib (Gleevec®), gefitinib (Iressa®), sunitinib (Sutent®), sorafenib (Nexavar®), bortezomib (Velcade®), bevacizumab (Avastin®), trastuzumab (Herceptin®), cetuximab (Erbitux®), and mumab (Vectibix®), hormone therapies ing antiestrogens such as fulvestrant (Faslodex), tamoxifen, toremifine, ase inhibitors such as anastrozole, exemstane and letrozole, progestins such as megestrol e, and gonadotropin-releasing hormone and therapies such as antibodies against tumor specific antigens (e.g. prostate specific antigen, carcinoembryonic antigen, urinary tumor associated antigen, fetal antigen, nase (p97), gp68, TAG-72, HMFG, Sialyl Lewis Antigen, MucA, MucB, PLAP, estrogen receptor, laminin reeptor, erb B and p155) which may be conjugated to a drug or toxin (e.g. radionuclide, ricin A chain, cholera toxin, pertussis toxin). [01 64] Radiotherapy herapy, also called radiation therapy, is the treatment of cancer and other diseases with ionizing ion which may be used to treat localized solid tumors such as s of the skin, tongue, larynx, brain, breast or cervix, or may be used to treat cancers of the blood-forming cells (leukemia) and lymphatic system (lymphoma). Radiation therapy includes, without limitation, the use of y—rays, X-rays and/or the directed delivery of radioisotopes to tumor cells. Other forms of DNA damaging factors are contemplated such as microwaves and adiation. Dosage ranges for X—rays range from daily doses of 50-200 roentgens for prolonged periods of time (3 to 4 weeks), to single doses of 2000-6000 roentgens.

Radiotherapy also ses the use of radiolabeled antibodies to deliver doses of radiation directly to the cancer site (e.g. radioimmunotherapy, conformal radiotherapy), high resolution intensity modulated radiotherapy, and stereotactic radio-surgery. Stereotactic radio— y (gamma knife) for brain and other tumors employs precisely targeted beams of gamma radiotherapy from hundreds of different angles. Only one n, taking about 4—5 hours is required.

V. Pharmaceutical compositions Cells, Viruses, polypeptides, peptides, and compounds (i.e, therapeutic ) described herein can be administered as a pharmaceutical or medicament formulated with a pharmaceutically acceptable r. Accordingly, the therapeutic agents may be used in the manufacture of a medicament or pharmaceutical composition. Pharmaceutical compositions of the invention may be formulated as solutions or lized powders for parenteral stration. Powders may be reconstituted by addition of a suitable diluent or other pharmaceutically acceptable carrier prior to use. Liquid formulations may be buffered, isotonic, aqueous solutions. s also may be sprayed in dry form. es of suitable diluents are normal isotonic saline solution, standard 5% dextrose in water, or buffered sodium or ammonium acetate solution. Such formulations are especially suitable for eral administration, but may also be used for oral administration or contained in a metered dose inhaler or nebulizer for insufflation. It may be desirable to add excipients such as polyvinylpyrrolidone, gelatin, hydroxy cellulose, acacia, polyethylene glycol, mannitol, sodium de, sodium citrate, and the like.

Alternately, therapeutic agents may be encapsulated, tableted or prepared in an emulsion or syrup for oral administration. Pharmaceutically acceptable solid or liquid carriers may be added to enhance or stabilize the composition, or to facilitate preparation of the ition. Solid carriers e starch, lactose, calcium sulfate ate, terra alba, magnesium stearate or stearic acid, talc, pectin, acacia, agar or gelatin. Liquid carriers include syrup, peanut oil, olive oil, saline and water. The carrier may also include a sustained release material such as glyceryl monostearate or glyceryl distearate, alone or with a wax. The amount of solid carrier varies but, preferably, will be between about 20 mg to about 1 g per dosage unit.

The pharmaceutical preparations are made following the conventional techniques of pharmacy involving milling, mixing, granulating, and compressing, when necessary, for tablet forms; or milling, mixing and filling for hard gelatin capsule forms. When a liquid carrier is used, the ation may be in the form of a syrup, elixir, emulsion, or an aqueous or non-aqueous suspension. For rectal administration, the invention compounds may be combined with ents such as cocoa butter, glycerin, gelatin, or polyethylene glycols and molded into a itory. eutic agents may be formulated to include other medically useful drugs or biological agents. The therapeutic agents also may be administered in conjunction with the administration of other drugs or biological agents useful for the disease or condition to which the ion compounds are directed.

As employed , the phrase "an ive amount," refers to a dose sufficient to provide concentrations high enough to impart a beneficial effect on the recipient thereof. The specific therapeutically effective dose level for any particular subject will depend upon a variety of factors including the disorder being d, the severity of the disorder, the activity of the specific compound, the route of administration, the rate of clearance of the compound, the duration of treatment, the drugs used in combination or coincident with the compound, the body weight, sex, diet, and general health of the subject, and like factors well known in the medical arts and sciences. Various general considerations taken into account in determining the "therapeutically effective amount" are known to those of skill in the art and are described, e.g., in Gilman et al., eds, Goodman And Gilman‘s: The Pharmacological Bases of Therapeutics, 8th ed., Pergamon Press, 1990; and Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Co., , Pa., 1990. Dosage levels typically fall in the range of about 0.001 up to 100 day; with levels in the range of about 0.05 up to 10 mg/kg/day are generally applicable.

A compound can be administered parenterally, such as intravascularly, enously, intraarterially, intramuscularly, aneously, or the like. Administration can also be orally, nasally, rectally, transderrnally or inhalationally Via an aerosol. The compound may be administered as a bolus, or slowly infused.

EXAMPLES The following es as well as the figures are included to demonstrate preferred embodiments of the ion. It should be appreciated by those of skill in the art that the techniques disclosed in the examples or figures represent techniques discovered by the inventors to function well in the practice of the invention and thus can be ered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the disclosure, appreciate that many changes can be made in the c ments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

Example 1 — Phase I Clinical Study A Phase I, dose—escalation, two-arm clinical trial of conditionally replication- competent adenovirus (Delta-24—RGD) for treatment of malignant gliomas has recently been completed. Arm A of the study tested direct intratumoral ion of a single dose of Delta—24— RGD into a growing area of a recurrent glioma. Arm B tested intratumoral administration of a divided dose into the ion bed following removal of a recurrent glioma. The A arm study started at a dose of 1 x 107 Viral particles (vp) and escalated in half log increments through 3 x 1010 vp; no maximum tolerated dose (MTD) was reached because no dose-limiting toxicities were found, nor were any serious adverse events (SAEs) reported. The Phase I trial showed clear ce of tumor response and good al in both A and B arms after reaching the relatively low dose of 3 x 108 vp. The Phase 1 A arm data (n=24) shows a high response rate by MRI (30-40%), as evidenced by tumor shrinkage and characteristic changes ("signature changes") on MRI. The evidence that these signature changes are relevant is derived from pathology reports on surgically resected tumors. Two tumors were resected several months after Delta—24-RGD therapy in response to what appeared to be tumor progression. In both instances, ogists reported that the tumors were 80% and 90% dead (necrotic) with the remaining tumor infiltrated by predominantly CD8 T cells. As a practical , the signature changes on MRI elicited by intratumoral delivery of Delta—24-RGD can be used as an early indicator of clinical response without having to rely upon accurate measurements of tumor size.

DeltaRGD is an adenovirus that has been engineered to infect cells lacking the Rb tumor suppressor gene, a condition unique to tumor cells, with high selectivity. In theory, adenovirus propagation in the tumor mass results in a series of infection-replication—lysis- infection events that will generate a wave ofpropagation, to potentially ate the tumor mass.

Oncolytic Viruses such as adenoviruses, therefore, hold a unique promise of overcoming the resistance of gliomas to convention therapies and circumvent the inaccessibility of tumor for surgery and the trend of resistance of cancer cells to radiotherapy and chemotherapy. A goal of the Phase I study was to find the maximum tolerable dose of DeltaRGD administered by intratumoral injection and into the post-resection cavity in patients with recurrent malignant glioma. Results from this trial ed that the intratumoral injection of the virus instigated an initial phase of oncolysis followed by a delayed inflammatory (Thl polarized) response that ultimately ed in tumor shrinkage with complete regression in a subset of these patients.

This is the first evidence that patients with gliomas develop an immune response after exposure to oncolytic Virus such as DeltaRGD and that this immune response can be used as the basis for predicting s of the ent.

Although the results described herein were obtained in patients with high-grade gliomas, the y of tic viruses in general (and Delta-24—RGD Virus in particular) to replicate in multiple tumor types has been demonstrated, including t limitation breast carcinoma, prostate carcinoma, large cell lung oma and sarcomas. The data presented herein on the treatment of gliomas is expected to be relevant to the vast majority of other solid A finding that was completely cted and unique during the phase I clinical trial using DeltaRGD was that at least 2 patients who had been previously treated with (and ) lomide and radiation therapy had a minimum of what appeared to be complete radiographic responses based on serial magnetic resonance imaging (MRI) scans. These patients had classic tumor response by MRI; one showed dramatic changes on MRI without shrinking and upon uent resection, the tumor was reported by pathology to be 90% necrotic with the rest infiltrated with immune cells. One of these patients is still alive and well over 2 1/2 years after treatment with a single injection of the virus. This was a striking finding and what was more unexpected was that the 2 patients who had these complete responses also had serum levels of IL-12p70, which were highly elevated compared to the rest of the population within this phase I trial. These IL-12p70 levels of cytokine correlate very well with the hypothesis that a T-helper— one (Th-1) polarization is necessary for l action of the oncolytic adenovirus. In fact, all patients who responded to treatment with Delta-24—RGD had significantly elevated IL-12p70 levels prior to and after treatment with the virus (100% correlation). This data is also consistent with the finding, discussed below, that the serum of these patients with complete responses had very low levels of antibodies against cancer-related antigens. Moreover, analysis of resected tumors from patients that ded to the virus during the Phase 1 study demonstrated an ation of macrophages at two weeks post—treatment followed by CD8 T cells at several months.

This data suggests that stimulation of a Th1 phenotype (ie. high levels of Th1 nes such as IL-12) in a patient, for example by administering a Th1 cytokine such as human recombinant lL-12p70, lL-2, IFN—y or an agent which stimulates IL-12p70 production such as id or lenalidomide prior to or after administration of an oncolytic virus such as DeltaRGD could potentially be used to augment the patient’s se to the s of the virus. Thus, one patient with low baseline IL—12p70 levels was administered IFN—y 2 months after receiving DeltaRGD (2 million units of interferon gamma-1b (Actimmune®) subcutaneously on Monday, Wednesday and Friday, continuously) in order to produce a Th1 phenotype and accordingly t the anti—tumor effects of the virus. The patient ded very well to the virus and s to be a te responder, thus providing a proof of principle. Suppression of Th2 cytokines such as IL—4, IL—5, IL-10, IL-13 could also represent a novel strategy for augmenting the effects of oncolytic viruses.

The present inventors have also discovered that patients who had a high level of antibodies t cancer related antigens prior to receiving DeltaRGD or who developed an increase in circulating auto-antibodies to cancer antigens during tic virus therapy did not exhibit a response to the virus. Conversely, the serum of patients who were complete responders had very low levels of antibodies against cancer related antigens. A 100% correlation was again observed, consistent with the ation between IL-12p70 and response to the virus. Thus, a T- helper-one (Th-l) polarization is necessary for optimal action of the oncolytic adenovirus.

Briefly, sera of ts entering the Phase I clinical trial were tested for the presence or e of antibodies against 31 distinct cancer related antigens both prior to receiving 24-RGD and also for the pment of antibodies post-treatment. These cancer antigens ed cancer/testis antigens, a class of tumor antigens whose expression is normally restricted to germ lines but are activated in a wide range of cancer types, often encoding antigens that are immunogenic in cancer patients. Currently there are no systemic studies on the presence of these antibodies against cancer antigens in patients with glioma. The present inventors have obtained the first evidence that patients with glioma have developed an immune response against cancer ns. Surprisingly, serum from ts with tumors that had a radiographic response to DeltaRGD had low or no humoral antibody response to the defined set of antigens. The lack of antibody se in ts who respond to DeltaRGD is consistent with the requirement of a Th1 polarized immune system to respond to the virus.

These unique findings have extremely broad implications: (1) high levels of Th1 cytokines such as IL—12p70 and/or low levels of antibodies to cancer related ns provides a novel set of biomarkers which can be used to identify a subset of patients who will respond to oncolytic virus (6. g. adenovirus) therapy and (2) administration of agents which produce a Th1 immune phenotype (i.e. increase the levels of lL-12p70 and other Th1 cytokines) can be co- administered prior to or after administration of an oncolytic virus in order to augment oncolytic virus therapy. For instance, recombinant human lL-12p70 (or interferon-gamma or an agent which stimulates production of these cytokines such as Revlimid) could be administered to cancer patients systematically to augment the effect of oncolytic s such as adenovirus and thereby increase antitumor activity and e treatment outcome.

The present inventors have thus discovered that a predominant expression of Th1 cytokines can be used to predict the outcome of oncolytic virus therapy in patients with s such as gliomas. Without wishing to be bound by theory, it is proposed that a subset of patients with cancer having a predominantly Th1 cytokine profile are primed to mount a cell-mediated antitumor immnunoresponse. In particular, the present inventors have surprisingly discovered that glioma patients with measurable responses to 24-RGD in a positive clinical outcome have high levels of Th1 cytokines IL-12p70, IL—2 and IFN— 7 whereas a high level of antibodies against tumor ated antigens seems to predict patients who will not respond to the virus.

The patients that had a complete response or a high level of response had high levels of Th1 cytokine interleukin-12p70 pre—operatively (serum levels were highly elevated compared to the rest of the tion within the phase I trial) and this level increased after injection of the virus.

The two patients who had te ses also had serum levels of IL-12p70, which were highly elevated compared to the rest of the population within this phase I trial.

The role of other proinflammatory Th1 cytokines (e. g. IFN-y) and anti-inflammatory Th2 cytokines (e. g. IL-4 and IL—10) in the response of glioma patients to treatment with the oncolytic adenovirus DeltaRGD was investigated. The role of phosphoproteins (phospho— STAT3 (Tyr 705)) and cleaved caspase—3 were also ed. Phospho-STAT3 (Tyr705).

Thus, o—STAT3 (Tyr705) and d caspase-3 in cell lysates and GM-CSF, IFNy, ILlfi, IL—2, IL-6, IL—8, IL-10, IL-12p40 and TNFoc levels in cell culture media were determined using custom-coated multispot plates and 21 Sector Imager 6000 according to the manufacturer’s protocol (Meso Scale with minor modifications). For quantitation of cytokines, cell culture media were ted and stored at -80°C. For cleaved caspase-3 and phospho-STAT3 (Tyr 705) tation, cells subjected to experimental manipulations were washed with ice-cold phosphate-buffered saline and then lysed by placing on ice in MSD complete lysis buffer (150 mMNaCl, 20 mMTris [pH 7.5], 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 10 mMNaF, MSD phosphatase inhibitor 1, MSD atase inhibitor II, and Protease Inhibitor Cocktail) with occasional vortex—mixing for 30 min. Following centrifugation at 17,968 g for 10 min at 4°C the supernatant was collected, and the protein tration was determined and the cleared lysates were stored at -80°C.

Electrochemiluminescence assays were performed on biological duplicate samples using capture antibody precoated 96-well multispot plates from Meso Scale Discovery (MSD; Gaithersburg, MD). Briefly, plates were blocked for 1 hour at room temperature with shaking and washed four times with Tris-buffered saline with 0.1% Tween-20. Fifteen micrograms of protein or twenty—five microliters of atant or calibrator was added to each well and incubated with shaking for 1 hour at room temperature or overnight at 4°C. Plates were washed and then specific protein levels were tated by adding 25 ul of 1 ptng specific detection antibody labeled with MSD SULFO-TAG reagent to each well and incubated with shaking for 2 hours at room temperature. Plates were washed four times with Tris-buffered saline with 0.1% Tween—20 as , 150 pl of 2x or 1 X read buffer was added, and the plates were immediately read using the SECTOR Imager 6000, and data were quantitated using ery Workbench and SOFTmaX PRO 4.0.

Data obtained to date has demonstrated that a predominant expression of Thl cytokines predicts a patient’s clinical outcome. Without being bound by , it is ed that a subset of patients with cancer having a predominantly Thl cytokine profile are primed to mount a cell-mediated antitumor immnunorespons. In particular, the present study demonstrates a Thl -driven immune response by cytokines IL-12 and IFN—y in glioma patients correlates with a measurable response to DeltaRGD and positive clinical outcome, s a high level of antibodies against tumor asociated antigens seems to predict patients who will not respond to the Virus. The response of Th2 cytokines IL-4 and IL-10 are not involved during DeltaRGD ent and their expression levels do not appear to be relevant for clinical outcome. Thus, a skew of the immune response from humoral (as measured by e.g. autoantibody titration) to cellular (as documented e.g. by cytokine analyses) can be used to predict ts that are likely to be responders to oncolytic s such as DeltaRGD.

An alternative or additional strategy for augmenting the effects of tic viruses such as DeltaRGD is to decrease the level of or suppress the T—regulatory cells also known as T—regs which can be accomplished by a number of herapy agents, including a number of alkylating agents such as Temozolomide and cyclophosphamide. The highly Th1 polarized immune systems prior to and during therapy in the two complete responders who had previously failed temozolomide therapy indicates that an agent that sses T-regulatory cells could be administered to a glioma or other cancer patient prior to oncolytic virus therapy in order to prime the patient’s immune system to respond to the virus. Alternatively, an agent that suppresses T- regulatory cells could be administered after administration of the virus.

Taking advantage of the discoveries described herein, a "perfect storm" treatment is envisioned or embodied by the use of an tic virus such as DeltaRGD comprising (a) ministration of an IL—12 stimulating agent such as recombinant human lL~12p70 or IFN-y or agents such as Revlimid (lenalinamide) to stimulate IL—12p70 production prior to administering the virus (b) injection of the virus into multiple areas of the tumor to achieve maximal sis and optionally (c) administering to the patient an agent which can reduce the T~reg population in the proliferate case and/or stimulate a cell mediated immune response. For example, Temozolomide can be used to reduce T-reg populations in a potentially a dose-dense fashion which would be 7 days on and 7 days off or 21 days on and 7 days off or standard dosing, five days on, 23 days off of the drug. This combination therapy would first increase Th1 cytokines, which drive the Th1 or T-helper 1 response, which mediates T-cell effector cells. The introduction of DeltaRGD into the tumor mass then creates an en burst" of both viral ns and presumably cancer—related ns, as the tumor cells are lysed. Finally, administration of agents that stimulate a cell mediated immune response against tumor related antigens such as CTLA-4 antagonists such as Ipilimumab and PD-l/PD—Ll receptor antagonists stimulate and keep the T-cells mediated clones replicating and active.

Example 2 — Phase IB clincial trial: inistration of an oncolytic Virus with a Th1- stimulating agent A phase 1B clinical study is underway designed to determine the maximum ted dose of DeltaRGD, administered by intratumoral injection and into the post-resection cavity concurrent with Ipilimumab, stered intravenously, as well as the dose limiting toxicities and anti-tumor activity of the combination.

Ipilimumab is a fully human cytotoxic T—lymphocyte antigen (CTLA—4)—blocking Igle monoclonal antibody (formerly MDX—OlO). CTLA—4 is a negative regulator of T cell activation. CTLA-4 functions as an immune checkpoint by exerting an inhibitory control on T cell activation and blocking this particular pathway. Blockade with ipilimumab allows the immune response to persist. Ipilimumab binds to CTLA—4 and blocks interaction of CTLA-4 with its ligands D86. CTLA-4 blockade has been shown to augment T-cell tion and proliferation; the ism of action of umab may occur through T-cell mediated anti tumor immune responses. Briefly, ipilimumab blocks the regulatory ck loop mediated by CTLA-4 and thereby effectively stimulates T-cell eration and secretion of lL—2.

Ipilimumab has gained tory approval by the FDA for treatment of unresectable or atic melanoma. During a Phase 3 clinical trial in which patients with these cancers were administered either gp100 (a peptide vaccine comprising a melanoma—associated antigen), ipilimumab or a combination of both. Patients d with ipilimumab alone had a 34% ion in risk of death over the gp100 arm but no ence in median overall survival (OS) was observed between ipilimumab alone and the combination of gp100 + ipilimumab.

Patient Eligibility — Inclusions Patients with histologically proven recurrent malignant y glioma will be eligible. Glioma type will be restricted to: stoma multiforme (GBM) and gliosarcoma (GS). Patients will consent to have a biopsy taken at the time of the stereotactic injection to confirm the presence of malignant glioma (based on frozen section) before injection of Delta-24— RGD-4C. Patients must be g and able to give informed consent. Patient age must be 3 18 years. Patients must have a Karnofsky performance status >/= 60. Patients must have recovered from the toxic effects of prior therapy (i.e. CTC grade 1 or less) - for example, they must be at least 2 weeks after vincristine, 6 weeks after nitrosoureas, and 3 weeks after procarbazine or Temozolomide administration. Patients must have adequate bone marrow function (absolute granulocyte count 3 1,500 and platelet count of 3 75,000), adequate liver function (SGPT and alkaline phosphatase 5 2 times institutional normals and bilirubin <1 .5 mg%), and adequate renal function (BUN or creatinine <1 .5 times institutional normal) prior to starting therapy. This study was designed to include women and minorities, but was not designed to measure differences of intervention s. Males and females will be recruited with no preference to . No exclusion to this study will be based on race. Minorities will actively be recruited to ipate.

Exclusions Excluded from the study will be patients with: (1) Active uncontrolled infection or unstable or severe intercurrent medical conditions. All patients must be afebrile at baseline (i.e., < 380° Celsius [C]). (2) Evidence of bleeding sis or use of anticoagulant tion or any medication that may increase the risk of bleeding that cannot be stopped prior to surgery. If the medication can be discontinued 3 1 weeks prior to DeltaRGD-4C injection then patient may be eligible. (3) History or current diagnosis of any medical or psychological condition that in the Investigator's opinion, might interfere with the subj ect’s ability to participate or inability to obtain informed consent because of psychiatric or complicating medical problems. (4) Female who is pregnant and/or nursing. Because of the potential risk of a recombinant virus ning a gene involved in ar growth regulation and differentiation which could potentially affect a developing fetus or growing infant, females who are nt, at risk of pregnancy, or breast g a baby during the study period are excluded. (5) Immunocompromised subjects, subjects with autoimmune conditions, active hepatitis (A, B, C or D [Delta]) or HIV sitivity. (6) Patients with Li-Fraumini Syndrome or with a known germ line deficit in the retinoblastoma gene or its related pathways. (7) Multiple intracranial ant glioma lesions. (7) Documented extracranial metastasis. (8) Biologic/immunotherapy (e.g., lL-2, IL—12, interferon) within 2 weeks of DeltaRGD-4C administration. (9) Any indication for undergoing MR1 such as: individuals with pacemakers, epicardial pacer wires, infusion pumps, surgical and/or aneurysm clips, shrapnel, metal prosthesis, implants with potential magnetic properties, metallic bodies in the eyes, etc. (10) White blood cell (WBC) < 2.5 x 103/mm3, absolute neutrophil count (ANC) < 1.5 x 103/mm3, platelet < 75,000/mm3, hemoglobin (Hgb) E 10.0 gm/dL, prothrombin time/international ized ratio (PT/INR) or l thromboplastin time (PTT) > 1.8 x control. (11) Grade 4 hematological toxicity. (12) Serum creatinine > 1.5 mg/dL. (l3) Liver transaminases (aspartate aminotransferase [AST] and/or alanine aminotransferase [ALT]) or total bilirubin > 2x the upper limits of normal. (14) Current diagnosis of other cancer except curative cervical cancer in situ, basal or squamous cell carcinoma of the skin. (15) History of encephalitis, le sclerosis, other CNS ion or primary CNS disease that would interfere with subject evaluation. (16) Males or s who refuse to use a double- barrier form of birth control during the study and for up to 6 months after injection with Delta— 24-RGD-4C ent Plan - Study Overview This study will have a limited phase IB component with the combination of ipilimumab with Delta—24-RGD-4C for the ent of patients with recurrent glioblastoma.

Study patients will require biopsy-confirmed recurrent tumor and will allow two prior therapies or two prior tumor recurrences.

Patients with biopsy-confirmed first or section recurrence of GBM will be consented and administered an l intratumoral or resection bed injection of 3 X 1010 vp Delta—24-RGD in 1 ml. Patients will be monitored for viral shedding at a variety of time points and for tumor response by MRI at two and four weeks to look for "signature" evidence of tumor destruction on MRI. Patients who show no evidence of changes on MRI will be offered an additional dose of Delta—24-RGD, followed by the option of a third dose at two months post treatment. This strategy will control for the possibility that Delta—24—RGD may be ‘delivered sub-optimally during a single injection. Sub-optimal delivery could be due to: (1) missing or not selecting an enhancing area of the tumor, (2) reflux of virus out of the tumor due to intratumoral pressure, (3) genetic alterations in the tumor that make it unable to support robust DeltaRGD replication, or (4) immune suppression due to prior toxic chemotherapy or corticosteroids that may affect the ability of Delta-24—RGD to establish an mor response.

All patients will be monitored for tumor response, safety, ssion—free and overall survival and quality of life ments. s all of the standard blood measurements, including Ad seroconversion, we will also g the appearance (or disappearance) of serum anti-tumor antibodies using a proprietary assay developed by trix (Carlsbad, CA), as well as cytokine assays.

Dose Escalation — Phase I The dosing of DeltaRGD-4C ,will be given at one log less than the highest dose which was safely given during the previous phase I study of 24—RGD-4C as a single agent, that being a dose of 3 x 109 viral particles per ml given as a one m1 ion into the tumor bed by a stereotactic framed delivery system. (The highest dose given during the previous phase I single-agent study was 3 X 1010 viral particles per ml). The phase I component will be based on a 3 x 3 design, whereas if no toxicity is seen in the first 3 patients greater than grade 1 or 2, then the ts may escalate to the next dose which will be 1 x 1010 viral particles per m1 given in 1 m1 injection volume, again by stereotactic framed ry method. Again, if there is no toxicity greater than 1 or 2 at this dose level then we will reach the maximum dose for this trial which will be 3 x 1010 viral les per m1 given on day 28 after starting the temozolomide dosing.

The patients will be allowed to have up to 3 total injections of the virus on subsequent monthly courses 2 and 3, again given at day 21 after starting a new course of temozolomide. Once the highest dose combination is reached, assuming there is no toxicity greater than grade 1 or 2, then the phase II component of this combination clinical trial will commence.

All Subjects After Virus administration and surgical procedures, all subjects will be transferred to the ICU until stable. Subjects may then be transferred to an ient or Step-Down unit based on the y surgeon’s decision. After 3 days, final discharge of subjects will be at the discretion of the Investigator. Subjects will be placed on contact isolation for the duration of their post-operative hospitalization and managed per institutional policies for adenovirus Dosing and Dose-Escalation Safety Stopping Rules - The primary safety variable will be the proportion of subjects who experience any grade 3 or greater toxicity that are at least possibly related to Delta RGD-4C (and not to the surgical procedure).

The toxicity rate will be no greater than 30% for any dose level. If the number of subjects who experience grade 3 or greater toxicity that are deemed to be at least possibly related to DeltaRGD-4C is greater than or equal to the t probabilities above who received DeltaRGD—4C, then the dose will be considered too toxic and the subjects exhibiting DLT attributable to DeltaRGD~4C will be reviewed by Principal Investigator. onal stopping criteria e (a) death that is at least possibly attributable to DeltaRGD-4C, (b) severe allergic reactions at least possibly attributed to DeltaRGD- 4C (CTC Grade 3 or 4), and/or (c) DLT in 3 two patients at the lowest dose level.

Dose Interruption — 24—RGD—4C administration should be upted if the subject develops symptoms of decreased cerebral on or cardiovascular perfusion or if there are signs of allergic reaction or anaphylaxis. Signs or symptoms of anaphylaxis include rash, hives, changes in blood pressure, and shortness of breath in subjects not under general anesthesia. DeltaRGD-4C administration will also be interrupted for any adverse event that, in the Investigator's opinion, warrants uption. In addition, if it appears at that ventricular penetration has occurred, the procedure will be aborted and no further DeltaRGD-4C will be administered.

Corticosteroids and Anticonvulsants - Decadron will be used in the study because the greatest challenge in viral gene therapy has been the immune response to the virus itself.

Steroids will also control brain inflammation and edema. The total daily steroid dose (expressed in mg decadron/day) will be recorded the day before y. The dose will be adjusted at the discretion of the Investigator, with the exception that all subjects will receive 10 mg IV just prior to surgery. "Surgery" includes both stereotactic injection and open tomy. Efforts will be made to reduce the d dose to the l clinically efficacious dose. Anticonvulsants will be administered at the discretion of the Investigator.

Excluded Therapy - Subjects may not receive any of the following medications or therapies while on-study ing a DeltaRGD-4C injection: (1) Vaccinations (2) Gene transfer therapy Dose Modification for Ipilimumab — Recommended Dose Modifications per Prescribing information. Withhold dose for any moderate immune-mediated e reactions or for symptomatic endocrinopathy until return to baseline, improvement to mild severity, or complete tion, and patient is receiving <7.5 mg prednisone or equivalent per day. ently discontinue YERVOY for any of the ing (1) Persistent moderate e reactions or inability to reduce corticosteroid dose to 7.5 mg prednisone or equivalent per day (2) Failure to complete full treatment course within 16 weeks from stration of first dose (3) Severe or life-threatening adverse reactions, including any of the following: (i) Colitis with abdominal pain, fever, ileus, or peritoneal signs; increase in stool frequency (27 over baseline), stool incontinence, need for intravenous hydration for >24 hours, gastrointestinal hemorrhage, and intestinal perforation (ii) AST or ALT >5 X the upper limit of normal (ULN) or total bilirubin >3 X the ULN (iii) Stevens-Johnson syndrome, toxic epidermal necrolysis, or rash complicated by full-thickness dermal ulceration or necrotic, bullous, or hemorrhagic manifestations (iv) Severe motor or sensory neuropathy, in-Barré syndrome, or myasthenia gravis (v) Severe immune-mediated reactions involving any organ system (vi) Immune-mediated ocular disease unresponsive to topical immunosuppressive therapy.

Study ures All patients must have signed the IRE-approved informed consent form before the initiation of any study-related evaluations. In addition, the institution’s surgical consent form for ve procedures will be signed.

Patients will have a complete history (including details of prior tumor therapy and concurrent non-malignant disease) and evaluation of all neurological symptoms within 2 weeks prior to the Day O/Baseline ure. Additionally, they will undergo a complete physical examination including a general examination and a neurological examination. Vital sign measurements and weight will be recorded and Kamofsky mance status score determined.

Routine baseline laboratory g will occur within two weeks of Day 0/Baseline procedure.

These tests will be evaluated as baseline and for surgical clearance.

Finally, all subjects will undergo MRI of the brain with and without gadolinium administration within two weeks prior to the Day O/Baseline procedure. Preliminary decisions regarding the eligibility of the subject for gene transfer therapy and the injections and/or procedures required for each subject will be decided based on the scan results.

Special Pretreatment Laboratory Assessments — All subjects will o special laboratory studies; Serum will be tested for anti-AdVS antibody titer (ELISA); Additional serum will be tested for antibodies to specific cancer-related antigens (CRA) as well as cytokine profile using ELISA based MSD; Peripheral blood will be subject to flow cytometry (at the PI. discretion); Serum pregnancy testing will be med for females of childbearing potential; HIV-1 testing will be performed on all subjects; Hepatitis screening serology: hepatitis A, B [core antibody and surface antigen], C and D [Delta virus]; The following will be used to test serum, nasopharyngeal secretions, and urine for viral dissemination; and PCR for AdV DNA specific to Delta—24-RGD-4C and wild—type AdV.

Evaluation after Surgical Procedures - Within 36 hours after stereotactic biopsy, subjects will have a CT scan to assess for the presence of hematoma. While the subject is in the hospital, daily assessments will be conducted including short ogic evaluation, sky mance status,vital sign ements, determination of adverse signs or symptoms (clinical toxicity) and osteroid dose. On the day after all al procedures serum, urine, and nasopharyngeal secretions will be collected for monitoring Virus dissemination and serum anti-AdVS antibody titer determination. Within 24 hours after a ure, subjects will have serum chemistry and hematological studies performed. tion after Craniotomy: An MRI of the brain with and without gadolinium will be performed for all subjects within 48 hours after craniotomy to assess the extent of resection and/or adverse s of intramural injection.

Evaluation after Discharge: Patients can be discharged within 3 days of the procedure.

Subjects will then be evaluated on day 28 (+/- 2 days) and months 2 (+/— 4 days), 3 (+/— 4 days), and 4 (+/- 4 days). Thereafter, follow—up will occur every two months (+/— 7 days).

Follow-Up Assessments ~ During outpatient follow—up, each subject will undergo a complete physical and ogical examination, assessment of Karnofsky performance status, weight, vital sign measurements, determination of adverse events and an evaluation of steroid and concomitant medication use. An MRI of the brain, without and with gadolinium will be performed. Clinical safety labs will also be performed and samples of serum, urine, and nasopharyngeal secretions obtained for the shedding of systemic AdV. y, serum will be ted for anti-AdVS antibody titer determination.

Specimen Analysis — All laboratory tests will be recorded on the appropriate CRF/worksheet record or electronic database. If unscheduled tory tests are performed based on clinical judgment, the results of these tests must also be recorded. Biopsy samples will be collected at the time of stereotactic . One portion of the biopsy specimen will be frozen in OCT media and another will be fixed in formalin and paraffin—embedded. Remaining biopsy samples will be flash-frozen. The tumor biopsy en will be ed for the ing: H & E ng Specimens for Virus Dissemination Studies - Virus shedding and dissemination will be monitored at the indicated time points in nasopharyngeal secretions, urine and serum. Virus dissemination will be ined by PCR analysis of DeltaRGD-4C and wild—type AdV DNA, and culture as required, by noting the appearance of a CPE in a 293 cell-based assay following the addition of a subject sample. The 293 cell line provides the El protein in trans, thus ting the replication of El-deficient Delta—24—RGD-4C. Confirmation of CPE in select samples as adenoviral-derived will be determined by an ELISA-based assay for hexon protein. Samples will be tested for the presence of replication-competent irus (RCA) by a CPE assay in a 293, cell line that does not provide the E1 proteins in trans.

Statistics The y objective of the phase I portion of this study is to determine the MTD for the combination of ipilimumab and 24-RGD.

Phase IzThree patients are entered per dose level if no DLT is encountered in these 3 patients. If DLT is encountered, the cohort will be expanded to 6 patients. A maximum of 4 higher-dose s are expected. If DLT is found at the starting dose, a maximum of two lower-dose cohorts can be explored. At least 6 patients will be treated at the recommended dose (or MTD-1) level. Hence, at least 9 and m 12 (two dose levels of virus escalation) patients will be treated in this portion. Patients treated at the MTD in the phase 1 portion of this study the number of subjects may be expanded for a preliminary Phase II component.

For the current single—stage trial, the hypotheses to be tested are H0: p < p0 versus H1: p > p1, where p is the probability of remaining alive and free from progression at 6 months. If the value of p0 (uninterestingly low response) will be set at 20% and pl at 40%, a trial with 40 patients will give acceptable error rates for the hypothesis g and precision for estimation (with alpha of 5% and power of 87%). The trial will be successful (null hypothesis rejected) when 13 or more patients are alive and progression-free at 6 months.

To assure adequate l of evaluable patients, the sample size will be se by % (4 additional patients). Therefore a total of 44 patients will be enrolled. e Events Definitions For this protocol, an adverse event (AB) is any untoward medical occurrence (e.g., sign, symptom, disease, syndrome, urrent illness, abnormal laboratory finding) that emerges or s relative to pre-injection baseline, during the administration or follow-up periods in a subject in a clinical investigation who has been administered an investigational product. The untoward medical occurrence may not necessarily have a causal onship to the administration of the product. An AE can therefore be any rable and/or unintended sign (including an abnormal laboratory result), symptom, or disease temporally associated with the use of a medicinal (investigational) product, whether or not related to the medicinal (investigational) product.

Common events/symptoms surrounding surgical procedures (e.g., pain, headache, standard blood pressure variations even if requiring antihypertensive medication, constipation, etc.) are within normal ce. Chronic, underlying, disease-related conditions that remain ged from baseline will not be considered as AEs. Exacerbations of underlying chronic conditions will be assessed for "seriousness" and if they are determined to be "serious" using the regulatory definition, will be reported as Serious Adverse Events (SAEs) on the appropriate forms.

Disability: A substantial disruption of a person’s ability to conduct normal life functions.

Life-threatening adverse event: Any adverse drug experience that, in the view of the Investigator, places the t at immediate risk of death from the reaction as it occurred, i.e., it does not include a reaction that, had it occurred in a more severe form might have caused death.

Unexpected adverse event: Any adverse drug ence, the specificity or severity of which is not consistent with the current Investigator Brochure; or if an Investigator Brochure is not required or ble, the specificity or severity of which is not consistent with the risk information described in the general investigational plan or elsewhere in the current application, as amended. For e, under this ion, hepatic necrosis would be unexpected (by virtue of greater severity) if the Investigator Brochure or other product literature only referred to elevated hepatic enzymes or hepatitis. Similarly, cerebral vasculitis would be cted (by virtue of greater specificity) if the Investigator Brochure only listed cerebral vascular accidents. ected," as used in this definition, refers to an adverse diug experience that has not been previously observed (e.g., included in the igator Brochure) rather than from the perspective of such an experience not being anticipated from the pharmacological properties of the investigational product.

Associated with the use of the Investigational drug (Causality i.e., investigational study drug—related): There is a reasonable possibility that the experience may have been caused by the investigational agent. The Principal Investigator must review each adverse event and determine whether or not it is related to the investigational agent.

Serious Adverse Events - An adverse event occurring at any dose (including overdose) should be classified as SERIOUS if: (1) It resulted in death (i.e., the AE caused or led to death) (2) It was life-threatening (i.e., the AE placed the subject at immediate risk of death; it does not apply to an AB that hypothetically might have caused death if it were more severe) (3) It required or prolonged in—subject hospitalization (i.e., the AE required at least a 24-hour in- subject hospitalization or prolonged a hospitalization beyond the expected length of stay.

Hospitalizations for ve medical/surgical procedures, scheduled treatments, or routine check-ups are not SAEs by this criterion)NOTE: The illness leading to the al or stic procedure should be ed as the AE or SAE, not the procedure itself. The procedure should be captured in the case narrative as part of the action taken in response to the illness.

It was disabling (i.e., the AE resulted in a substantial disruption of the subject’s ability to carry out normal life functions) It is a congenital anomaly/birth defect (1.6., an adverse outcome in a child or fetus of a t exposed to the molecule or Investigational drug before conception or during pregnancy) It does not meet any of the above s criteria but may jeopardize the subject or may require l or surgical intervention to prevent one of the outcomes listed above (i.e., is a significant or important medical event) Note: The causality of the serious Adverse Events must also be determined given that the patients will be undergoing invasive surgical procedures that can in and of themselves lead to rd events or poor outcomes. Only those events deemed possibly or probably related to the study drug will be considered as DLTs.

Serious adverse events will be captured from the time the t signs consent until 30 days after the last dose of drug. Serious e events must be followed until clinical recovery is complete and tory test have returned to baseline, progression of the event has stabilized, or there has been acceptable resolution of the event. [023 7] Safety All subjects who received any Delta—24-RGD—4C will be included in the safety analysis. Subjects who do not complete the study for whatever reason will have all available data up until the time of ation ed in the safety analysis. e events will be summarized both overall and by dose group and tabulated by severity, relationship to Delta—24-RGD-4C and causality. The number and percentage of subjects experiencing adverse events will be tabulated by body system/preferred term both overall and by dose group. When an adverse event occurs more than once, the maximum severity and causality will be counted. Additionally, serious e events, adverse events that are possibly related to DeltaRGD-4C and adverse events that are unrelated to Delta RGD-4C will be summarized separately. Concurrent illnesses will be listed and may be examined as le confounders in the treatment response relationship. Concomitant medications and therapies will also be listed, as will previous treatments for malignant .

Any potentially related side effects will be analyzed. Data listings for all adverse events will be provided by subject.

Vital Sign Measurements — Vital sign measurements (blood pressure, heart rate, atory rate and temperature) results will be presented in data listings by visit, dose cohort and time interval, as appropriate. y data including univariate tics of mean, standard error and median will also be presented.

Physical and Neurological Examinations/Performance Status - Physical findings will be summarized for each subject by visit and dose cohort. Longitudinal analyses of tumor-related neurological symptoms, performance status and weight may also be performed. Summary data including univariate statistics of mean, standard error and median will also be presented as riate. Time to disease progression and performance status less than 60 will also be evaluated.

MRI Scans - MRI scans will be evaluated by the Investigator and descriptive statistics will be reported. Results will be reported by t visit and dose cohort and/or as riate.

Clinical Laboratory Results - Descriptive statistics for selected laboratory parameters will be presented overall and by subject by study day and dose group. For the same laboratory parameters, shift tables may be presented showing the number and t of subjects with high, normal, and low (or normal/ abnormal) laboratory results at baseline and post-Delta—24-RGD—4C injection by dose cohort. Group means, medians and rd errors will be calculated for the various laboratory ters. Laboratory values will also be listed by subject and those ing a normal reference range will be flagged.

Efficacy In this study, tumor response will be evaluated according to the MacDonald criteria (i.e. based on 2D measurements). In order to be comparable to previously published s, the thresholds for partial response and progressive disease are 50% and 25%, respectively.

Tumor response will be the change in the size of brain lesions from ne (within 4 days post DeltaRGD-4C administration) compared to 7 days post Delta—24-RGD-4C administration, 14, 21, 28 days, 2, 3 and 4 months and every two months thereafter following the last injection of virus. Changes in al disease status and steroid administration will be considered when reviewing changes in tumor size. Measurements obtained at these study time points will also be analyzed with consideration of any anti-tumor cancer therapies and timeframes administered. Brain tumor size will be calculated from MRI scans.

Subjects developing new rim enhancement in a previously non-enhancing area of the tumor bed (e.g. as is commonly seen on post-gadolinium Tl—weighted MRI beginning several days after a gross total ion) will not be considered to have developed ssive disease unless the maximum diameter of the enhancing rim exceeds 10 mm or an area of nodular enhancement develops. In cases judged to be indeterminate by the PI, a stereotactic biopsy may be performed to determine if progression has occurred. Additionally, measurement of the areas of contrast enhancement will be determined and compared to the sum of the products of perpendicular ers of all lesions using WHO criteria.

Time to disease progression will be calculated from the time of initiation of Delta RGD-4C administration at tumor injection as well as resection until evidence of progression by clinical exam, performance , and/or MRI. The Investigators Will read the MRI, as needed, for the purpose of clinical assessment.

Descriptive statistics will be used to summarize suivival data. According to Brem et al, the h historical survival rate is 35% for subjects with recurrent malignant glioblastoma.

The percentage of subjects with improved or stable symptoms at the end of the ion of each study phase will also be summarized using 95% confidence intervals. The change in Kamofsky score will also be summarized with univariate tics. Time to disease progression and KPS 5 60 may also be evaluated by the Kaplan—Meier analytical method.

Claims (12)

1. Use of: (a) an agent selected from IL-12p70, IL-2 and IFN- γ; and (b) an oncolytic virus selected from the group consisting of Delta-24 and Delta-24 in the manufacture of a medicament for treating cancer in a patient having or suspected of having cancer.

2. Use of: (a) an agent selected from IL-12p70, IL-2 and IFN-γ; and (b) an oncolytic virus selected from the group consisting of Delta-24 and Delta-24 in the manufacture of a medicament for ng cancer in a patient having or ted of having cancer, wherein the Th1 cytokine and the oncolytic virus are ated for administration to the patient separately.

3. The use of claim 1 or 2, where in the medicament further comprises an agent which suppresses regulatory T cells.

4. The use of claim 3, wherein the agent which suppresses regulatory T cells is selected from the group consisting of temozolomide (4-methyloxo- 2,3,4,6,8-pentazabicyclo [4.3.0] nona- 2,7,9-triene- 9-carboxamide), cyclophosphamide ((RS)-N,N-bis(2-chloroethyl)-1,3,2- hosphinanamine 2-oxide), lomustine (CCNU; N-(2-chloroethyl)-N'-cyclohexyl-N- ourea), bis-chloroethylnitrosourea (BCNU), melphalan hydrochloride (4 [bis(chloroethyl)amino]phenylalanine), and busulfan (butane-1,4-diyl anesulfonate).

5. The use of any one of claims 1-4, wherein the medicament is formulated for intratumoral administration.

6. The use according to claim 5, wherein said tumor is a brain tumor.

7. The use according to claim 6, wherein said brain tumor is a glioma.

8. The use of any one of claims 1-7, wherein the medicament is formulated for administration as a single dose.

9. The use of any one of claims 1-8, wherein the medicament is formulated for administration of the virus at a dose of from 1x106 to 1 x 1013 plaque forming units (pfu).

10. The use of claim 9, wherein the medicament is formulated for administration of the virus at a dose of from 1x108 to 1 x 1012 plaque forming units (pfu).

11. The use of claim 2, n the agent is formulated for administration to the patient prior to the virus.

12. The use of claim 2, n the agent is formulated for administration to the patient subsequent to the virus.