NZ335544A - Use of GPE (tripeptide or dipeptide) in form of Gly-Pro-Glu or Gly-Pro or Pro-Glu as a neuromodulator - Google Patents

Abstract

A method for regulating the effects of neural enzymes by administrating an effective amount of GPE or an analogue thereof. GPE is a tripeptide consisting of amino acids Gly-Pro-Glu. Administration of GPE relates to increasing the effective amount of neural enzymes choline acetyltransferase (ChAT), glutamic acid decarboxylase (GAD) and/or nitric oxide synthetase (NOS) in the central nervous system (CNS) for treating conditions such as motor neuron disease, Alzheimer's disease, muscular dystrophy, peripheral neuropathies, autonomic neuropathies, memory loss, neurodegeneration due to aging, post-asphyxial seizures, convulsive disorders, subarachnoid hemorrhage, transient ischemic attack, stroke, multi-infarct dementia, cerebral vasculitis or traumatic brain injury.

Description

REGULATION OF NEURAL ENZYMES This invention relates to methods of regulating the effect of neural enzymes, ll particularly relates to Increasing the effective amount of the neural enzymes choline 5 acetyltransferase (ChAT), glutamic acid decarboxylase (GAD) and nitric oxide synthetase (NOS) in the central nervous system (CNS).

BACKGROUND OF THE INVENTION GPE is a tnpeptide consisting of amino acids Gly-Pro-Glu. It and its dipeptide analogs Gly-Pro and Pro-Glu were first disclosed by Sara et al in EP 0366638. The suggestion made by Sara et al is that GPE has neuromodulator properties (the capability of affecting the electrical properties of neurons). GPE has also been established as having neuroprotective properties and therefore having utility m the prevention or inhibition 15 of neural cell death (WO 95/17204).

To date however, there has been no teaching or suggestion of GPE or its analogs having any direct effect on the effective amount of neural enzymes present in the CNS. There has certainly been no suggestion of GPE having the ability to upregulate expression of 20 the neural enzymes, ChAT, GAD and NOS, and/or of their receptors.

ChAT is involved m the synthesis of the neurotransmitter acetyl choline. An ability to upregulate ChAT expression therefore has implications for neural, muscular and neuromuscular therapy and prophylaxis, including where the survival of neural cells 25 is not threatened.

GAD is involved in the synthesis of the important inhibitory neurotransmitter gamma ammo butyric acid (GABA). An ability to upregulate GAD expression therefore has implications for neural therapy and prophylaxis.

NOS has multiple functions in the brain, including regulating blood flow, cell metabolism and cell survival. An ability to regulate NOS expression using GPE therefore has implications for neural therapy and prophylaxis, including where the survival of neural cells is not threatened.

Printed from Mimosa It is the object of this invention to provide new approaches to neuronal therapy or prophylaxis which involve directly upregulating the expression of neural enzymes present in the CNS, or at least to provide the public with a useful choice.

SUMMARY OF THE INVENTION In a first aspect, the invention provides a method of treatment of a patient suffering from or susceptible to a condition m which an increase in the amount of a neural enzyme selected from ChAT, NOS and GAD is desirable, which method comprises the 10 step of increasing the effective amount of GPE or an analog thereof withm the CNS of said patient.

In a further aspect, the invention provides a method of increasing the amount of the neural enzyme ChAT in a patient for therapy or prophylaxis of a neurological disorder 15 or condition, said method comprising the step of increasing the effective amount of GPE or an analog thereof within the CNS of said patient.

In still a further first aspect, the invention provides a method of increasing the amount of the neural enzyme GAD in a patient for therapy or prophylaxis of a neurological 20 disorder or condition, said method comprising the step of increasing the effective amount of GPE or an analog thereof withm the CNS of said patient.

In yet a further aspect, the invention provides a method of increasing the amount of the neural enzyme NOS in a patient for therapy or prophylaxis, said method comprising 25 the step of regulating the effective amount of GPE or an analog thereof within the CNS of said patient.

"Increasing the amount" of a neural enzyme is through upregulation of expression of the neural enzyme.

By "analog1 it is meant the dipeptides Gly-Pro and Pro-Glu as well as any other small peptide which is capable of effectively binding to the receptors in the CNS GPE binds to and of inducing an equivalent upregulatory effect upon the expression of ChAT, GAD or NOS and/or their respective receptors.

Prmted from Mimosa Most preferably, It is the effective amount of GPE itself which is increased within the CNS of the patient. This can be effected by direct administration of GPE and indeed this is preferred. However, the administration of compounds which indirectly increase the effective amount of GPE (for example a prodrugwhich, within the patient is cleaved 5 to release GPE) is in no way excluded.

The active compound (GPE or its analog) can be administered alone or, as is preferred, as part of a pharmaceutical composition.

The composition can be administered to the patient peripherally (for example by a parenteral route such as injection into the peripheral circulation) or can be administered directly to the CNS. This latter route of administration can involve, for example, lateral cerebro-ventncular injection or a surgically inserted shunt into the lateral cerebro ventricle of the bram of the patient.

Conveniently, the expression of ChAT and/or its receptors is upregulated through the administration of GPE or its analogs in the prophylaxis or therapy of one or more of the following: Motor neuron disease; Alzheimers disease; Muscular dystrophy; Peripheral neuropathies; Autonomic neuropathies; Memory loss; and 25 Neurodegeneration due to aging.

Conveniently, the expression of GAD and/or its receptors is upregulated through the administration of GPE or its analogs in the prophylaxis or therapy of one or more of the following: postasphyxial seizures; convulsive disorders such as epilepsy; and neurodegenerative diseases such as Huntingtons.

Printed from Mimosa Conveniently, the expression of NOS and/or its receptors is upregulated through the administration of GPE or its analogs in the prophylaxis or therapy of one or more of the following: subarachonoid haemorrahge; transient ischemic attack: stroke; multmfarct dementia; cerebral vasculitis; and traumatic brain injury.

In a further aspect, the invention also consists in the use of GPE or an analog thereof in the manufacture of a medicament for use in increasing the amount of ChAT, GAD or NOS present in the CNS.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is broadly as defined above. However, those persons skilled in the art will appreciate that it is not limited only to the above but that it also includes embodiments of which the following description provides examples. A better 20 understanding of the present invention will also be gained through reference to the accompanying drawings in which: Figure 1 shows the number of ChAT-positive neurons following treatment with a control vehicle or with 3 jig of GPE 2 hours after induced hypoxia; Figure 2 shows the number of GAD-positive neurons following treatment with a control vehicle or with 3 \ig of GPE 2 hours after mduced hypoxia; Figure 3 shows the number of NOS-positive neurons following treatment with a control 30 vehicle or with 3 \ig of GPE 2 hours after induced hypoxia, Figure 4 shows the effects of GPE on the number of GAD-positive neurons following quinohnic acid-induced injury to the brain; Printed from Mimosa Figure 5 shows the effects of GPE on the number of NOS-positive neurons following quinolinic acid-induced injury to the brain; and Figure 6 shows the effects of GPE on the number of ChAT-positive neurons following 5 quinolinic acid-induced injury to the brain.

DESCRIPTION OF THE INVENTION As indicated above, the present invention is broadly based upon the applicants 10 surprising finding that GPE and its analogs are capable of increasing the amount of certain neural enzymes within the CNS. This increase, which is through upregulating enzyme expression, is achieved through increasing the effective concentration or amount of GPE or the analog m the CNS of a patient. The neural enzymes specifically upregulated in effect are ChAT, GAD and NOS It is presently preferred by the applicants that GPE itself be used to increase the amount of the neural enzyme. Most conveniently, this is effected through the direct administration of GPE to the patient.

However, while this is presently preferred, there is no intention on the part of the applicants to exclude administration of other forms of GPE. By way of example, the effective amount of GPE in the CNS can be increased by administration of a prodrug form of GPE which comprises GPE and a carrier, GPE and the carrier bemgjomed by a linkage which is susceptible to cleavage or digestion withm the patient. Any suitable 25 linkage can be employed which will be cleaved or digested to release GPE following administration.

It is further preferred that GPE be administered as part of a medicament or pharmaceutical preparation. This can involve combination of GPE with any 30 pharmaceutical^ appropriate carrier, adjuvant or excipicnt. The selection of the carrier, adjuvant or excipient will of course usually be dependent upon the route of administration to be employed.

Printed from Mimosa WO 98/14202 PCT/NZ97/00132 The administration route can vary widely. An advantage of GPE is that it can be administered peripherally. This means that it need not be administered directly to the CNS of the patient in order to have effect in the CNS Any peripheral route of administration known in the art can be employed. These can include parenteral routes with injection into the peripheral circulation being a suitable example. However, alternative administration routes selected from oral, rectal, nasal, subcutaneous, inhalation, intraperitonial or Intramuscular can be employed.

Two of the most convenient administration routes will be by IV (eg. dissolved in 0.9% sodium chloride) or orally (in a capsule).

It will also be appreciated that it may on occasion be desirable to directly administer GPE to the CNS of the patient. Again, this can be achieved by any appropriate direct 15 administration route. Examples include administration by lateral cerebro-ventricular injection or through a surgically inserted shunt into the lateral cerebro-ventncle of the brain of the patient.

The calculation of the effective amount of GPE or its analogs to be administered will 20 be routine to those persons skilled in this art. Needless to say, the final amount to be administered will be dependent upon the route of administration and upon the nature of the neurological disorder or condition which is to be treated. A suitable dose range may for example be between about 0.04 fig to 1000 jig of GPE and/or analog per 100 g of body weight where the dose is administered centrally.

GPE and its analogs can be obtained from a suitable commercial source. Alternatively, GPE and its analogs can be directly synthesised by conventional methods such as the stepwise solid phase synthesis method of Merryfleld et al. (J. Amer. Chem. Soc. 85 2149-2156 (1963)) Alternatively, synthesis can involve the use of commercially 30 available peptide synthesisers such as the Applied Biosystems model 430A.

The present invention will now be illustrated with reference to the following non-limitmg examples.

Printed from Mimosa Example 1 The objective of these studies was to determine the effects of administering GPE on expression of ChAT in the presence or absence of CNS injury The experiment 5 involved treating the rats with a control vehicle or GPE 2 hours after a focal CNS injury. These rats had an hypoxic-ischemic injury to one cerebral hemisphere induced in a standard manner (ligation of the carotid artery). The degree and length of hypoxia, the ambient temperature and humidity were defined to standardise the degree of damage. The neuronal death is restricted to the side of the carotid ligation and is 10 primarily in the hippocampus, dentate gyrus, striatum and lateral cortex of the ligated hemisphere. There is no neuronal loss in the contralateral hemisphere.

Specifically, nine pairs of adult wistar rats (280-320g) were prepared under halothane/02 anaesthesia. The right side carotid artery was ligated A guide cannula 15 was placed on the dura 7.5mm anterior from stereotaxic zero and 1.5mm from midline on the right. The rats were allowed to recover for 1 hour and were then placed in an incubator with humidity 90+/-5% and temperature 31+/-0.5°C for 1 hour before hypoxia. Oxygen concentration was reduced and maintained at 6+/-0.202% for 10 minutes. The rats were kept in the incubator for 2 hours after hypoxia and then 20 treated either with 3ug GPE or vehicle alone (0.1 M citrate buffer [pH6], diluted 10 times in 0.1% bovine serum albumin in 0.1M phosphate buffered saline [PBS] lpH7.3]). A further 6 rats were used as normal controls. The rats were sacrificed using pentobarbital 3 days after hypoxic-schemic injury. Brains were perfused with normal saline and 4% paraformaldehyde and fixed in perfusion fixative overnight. 25 Brains were stored in 25% sucrose in 0.1M PBS (pH7.4) until the tissue sank. Frozen coronal sections (30um) of striatum, globus pallidus and substantia nigra were cut using a microtome and stored in 0.1% sodium azide in 0.1M PBS at 4°C. Immunoreactivity for Choline acetyltransferase (ChAT) was established by staining using a free floating section method. Briefly, the antibodies were diluted in 1% goat 30 serum. The sections were incubated in 0.2% triton in 0.1M PBS/triton at 4°C overnight before immunohistochemistry. The sections were pre-treated with 1% H202 in 50% methanol for 20 minutes. The sections were then incubated with rabbit (Rb) anti-ChAT (1:5000) antibodies (the primary antibodies) in 4D on a shaker for two days. The sections were washed using PBS/triton (15 minutes x 3d) and then incubated with goat Printed from Mimosa anti-rabbit biotmylated secondary antibodies (1:1000) at room temperature overnight. The sections were washed and incubated in (ExtrAvidin TM Sigma 1:1000) for 3 hours and followed by H202 (0.01%) m 3,3-diaminobenzidine tetraliydrochloride (DAB, 0.05%) reaction. These sections were mounted on chrome alum coated slides, dried, 5 dehydrated and covered.

The striatal neurons in both hemispheres which showed specific immunoreactivities corresponding to ChAT were counted using a light microscope and a 1mm 2x1000 grid. The size of the striatal region used for the count was measured using an image 10 analyser. The total counts of neurons/mm2 were compared between the GPE and vehicle treated group. Data were analysed with paired t-test and presented as mean +/- sem. Results are presented in Figure 1.

This figure shows that the number of ChAT immunoposilive neurons increased in both 15 the right and left (uninjured) sides. This clearly indicates that administration of GPE is effective to upregulate ChAT expression.

Example 2 The objective of these studies was to determine the effects of administering GPE on expression of GAD in the presence or absence of CNS injury The experiment involved treating the rats with a control vehicle or GPE 2 hours after a focal CNS injury. These rats had an hypoxic-ischemic injury to one cerebral hemisphere induced in a standard \ manner (ligation of the carotid artery). The degree and length of hypoxia, the ambient 25 temperature and humidity were defined to standardise the degree of damage. The neuronal death is restricted to the side of the carotid ligation and is primarily in the hippocampus, dentate gyrus, striatum and lateral cortex of the ligated hemisphere There is no neuronal loss in the contralateral hemisphere.

Specifically, mne pairs of adult wistar rats (280-320g) were prepared under haJothane/02 anaesthesia. The right side carotid artery was ligated. A guide cannula was placed on the dura 7.5mm anterior from stereotaxic zero and 1.5mm from midline on the right. The rats were allowed to recover for 1 hour and were then placed in an incubator with humidity 90+/-5% and temperature 31+/-0.5°C for 1 hour before Printed from Mimosa hypoxia. Oxygen concentration was reduced and maintained at 6+/-0.202% for 10 minutes. The rats were kept in the incubator for 2 hours after hypoxia and then treated either with 3ug GPE or vehicle alone (0 1M citrate buffer [pH6i, diluted 10 times in 0.1% bovine serum albumin in 0 1M phosphate buffered saline [PBS] 5 |pH7.3)). A further 6 rats were used as normal controls. The rats were sacrificed using pentobarbital 3 days after hypoxic-schemic injury. Brains were perfused with normal saline and 4% paraformaldehyde and fixed in perfusion fixative overnight. Brains were stored in 25% sucrose in 0.1M PBS (pH7.4) until the tissue sank. Frozen coronal sections (30um) of striatum, globus pallidus and substantia nigra were cut 10 using a microtome and stored in 0.1% sodium azide in 0.1M PBS at 4°C Immunoreactivity for GAD was established by staining using a free floating section method. Briefly, the antibodies were diluted in 1% goat serum. The sections were incubated in 0.2% triton in 0.1M PBS/triton at 4°C overnight before immunohistochemistry. The sections were pre-treated with 1 % H202 in 50% methanol 15 for 20 minutes. The sections were then incubated with rabbit (Rb) anti-GAD (1:5000) antibodies (the primary antibodies) in 4D on a shaker for two days. The sections were washed using PBS/triton (15 minutes x 3d) and then incubated with goat anti-rabbit biotmylated secondary antibodies (1:1000) at room temperature overnight. The sections were washed and incubated in (ExtrAvidin TM Sigma 1.1000) for 3 hours and 20 followed by H202 (0.01%) in 3,3-diaminobenzidine tetrahydrochloride (DAB, 0.05%) reaction. These sections were mounted on chrome alum coated slides, dried, dehydrated and covered.

The striatal neurons in both hemispheres which showed specific immunoreactivities 25 corresponding to GAD were counted using a light microscope and a 1 mm 2x1000 grid The size of the striatal region used for the count was measured using an image analyser. The total counts of neurons/mm2 were compared between the GPE and vehicle treated group. Data were analysed with paired t-test and presented as mean +/- sem. Results are presented in Figure 2.

This figure shows that the number of GAD immunopositive neurons increased in the right side whereas Parvalbumin (a marker for the same cell type) was not increased, showing that GAD expression was upregulated in the surviving cells (*p<0.05). ;-9- ;Prmted from Mimosa ;WO 98/14202 ;PCT/NZ97/00132 ;Example 3 ;The objective of these studies was to determine the effects of administering GPE on expression of NOS in the presence or absence of CNS injury. The experiment involved 5 treating the rats with a control vehicle or GPE 2 hours after a focal CNS injury. These rats had an hypoxic-ischemic injury to one cerebral hemisphere induced in a standard manner (ligation of the carotid artery). The degree and length of hypoxia, the ambient temperature and humidity were defined to standardise the degree of damage. The neuronal death is restricted to the side of the carotid ligation and is primarily m the 10 hippocampus, dentate gyrus, striatum and lateral cortex of the ligated hemisphere. There is no neuronal loss in the contralateral hemisphere. ;Specifically, nine pairs of adult wistar rats (280-320g) were prepared under halothane/02 anaesthesia. The right side carotid artery was ligated. A guide cannula 15 was placed on the dura 7.5mm anterior from stereotaxic zero and 1.5mm from midline on the right. The rats were allowed to recover for 1 hour and were then placed in an incubator with humidity 90+/-5% and temperature 31+/-0.5°C for 1 hour before hypoxia. Oxygen concentration was reduced and maintained at 6+/-0.202% for 10 minutes. The rats were kept in the incubator for 2 hours after hypoxia and then 20 treated either with 3ug GPE or vehicle alone (0.1M citrate buffer [pH6], diluted 10 times in 0.1% bovine serum albumin in 0.1M phosphate buffered saline [PBS] [pH7.3)). A further 6 rats were used as normal controls. The rats were sacrificed using pentobarbital 3 days after hypoxic-schemic injury. Brains were perfused with normal saline and 4% paraformaldehyde and fixed in perfusion fixative overnight. 25 Brains were stored in 25% sucrose mO.lM PBS (pH7.4) until the tissue sank. Frozen coronal sections (30um) of striatum, globus pallidus and substantia nigra were cut using a microtome and stored in 0.1% sodium azide In 0.1M PBS at 4°C. Immunoreactivity for neuronal nitric oxide synthetase (NOS) was established by staining using a free floating section method. Briefly, the antibodies were diluted in 30 1 % goat serum. The sections were incubated m 0.2% triton in 0.1M PBS/triton at 4°C overnight before immunohistochemistry. The sections were pre-treated with 1 % H202 in 50% methanol for 20 minutes. The sections were then incubated with rabbit (Rb) anti-NOS (1:3000) antibodies (the primary antibodies) in 4D on a shaker for two days. The sections were washed using PBS/triton (15 minutes x 3d) and then incubated with ;- 10- ;Pnnted from Mimosa ;WO 98/14202 ;PCT/NZ97/00132 ;goat anti-rabbit blotmylated secondary antibodies (1:1000) at room temperature overnight. The sections were washed and incubated in (ExtrAvidin TM Sigma 1 • 1000) for 3 hours and followed by H202 (0.01%) in 3,3-diaminobenzidine tetrahydrochloride (DAB, 0.05%) reaction. These sections were mounted on chrome alum coated slides, 5 dried, dehydrated and covered. ;The striatal neurons in both hemispheres which showed specific immunoreactivities corresponding to NOS were counted using a light microscope and a 1 mm 2x1000 grid. The size of the striatal region used for the count was measured using an image 10 analyser. The total counts of neurons/mm2 were compared between the GPE and vehicle treated group. Data were analysed with paired t-test and presented as mean +/- sem. Results are presented in Figure 3. ;This figure shows that the number of NOS immunopositive neurons increased in both 15 the right and left (uninjured) sides (#p=0.072, *p=0.008). This clearly indicates that administration of GPE is effective to upregulate NOS expression.

Example 4 The objective of this study was to determine the effect of GPE administration on the expression of GAD, NOS and ChAT in the presence or absence of injury to the striatum induced by quinolinic acid. When injected into the striatum m experimental animals quinolinic acid produces lesions arising from the loss of striatopallidal and striatonigral GABAergtc projection neurons, but spares the axons in the striatum 25 (Science, 219. 316-318 [1983]]. Rats were either treated with a control vehicle or GPE 2 hours after administration of quinolinic acid.

Materials and Methods A total of 14 adult male Wistar rats (280-3 lOg) were used in this investigation. Eight 30 rats were anaesthetized using halothane/02 anaesthesia. An injection of quinolinic acid (78 units, Sigma, dissolved in 4M NaOH) was stereotaxically placed in the dorsal region of the right striatum 0.8mm anterior to bregma, 2.5mm lateral to the midline and 4.0mm ventral to the pial surface. 2 hours later an injection of 3ng GPE or vehicle alone (0.1M citrate buffer [pH6], diluted 10 times in 0.1% bovine serum albumin in Printed from Mimosa 0.1M phosphate buffered saline [PBS](pH7.3]) was administered into the right lateral ventrical via a guide cannula 7.5mm anterior from stereotaxic zero, 1.5inm from the midline on the right, and vertical 3mm. Six rats were used as normal controls.

The rats were sacrificed using pentobarbital 3 days after the quinolinic acid induced striatal injury. Brains were perfused with 10% buffered formalin (pH7) and processed for immunoreactivity for GAD, NOS which are found m GABAergic interneurons in the striatum and ChAT, which synthesizes acetylcholine and is found in cholinergic neurons in the striatum. Brains were stored m 25% sucrose in 0.1M PBS (pH7.4) 10 until the tissue sank. Frozen coronal sections (30nm) of the striatum, globus pallidus and substantia nigra were cut using a microtome and stored m 0.1 % sodium azide in 0.1M PBS at 4°C. Immunoreactivity for GAD, NOS and ChAT was established by staining using a free floating method. Briefly, the antibodies were diluted in 1% goat serum. The sections were incubated in 0.2% tnton in 0.1M PBS/triton at 4°C overmght 15 before immunohistochemistry. The sections were pre-treated with 1 % H2Oz in 50% methanol for 20 minutes. The sections were then incubated either with rabbit anti-GAD (1:5000), rabbit anti-NOS (1:3000) or rabbit anti-ChAT (1:5000) in 4D on a shaker for two days. The sections were washed using PBS/tnton (15 minutes x 3) and then incubated with goat anti-rabbit biotinylated secondary antibodies (1.1000, 20 Amersham) at room temperature overnight. Sections were washed and incubated in (ExtrAvidin™, 1:1000, Sigma) for 3 hours and then reacted in 0.05% 3,3-diaminobenzidine tetrahydrochloride and 0.01% H,02 to produce a brown reaction product. These sections were mounted on chrome alum coated slides, dried, dehydrated and covered.

The striatal neurons in both hemispheres which showed specific immunoreactivities corresponding to GAD, NOS and ChAT were counted using a light microscope and a 1mm 2x 1000 grid. The size of the striatal region used for the count was measured using an image analyser (Mocha image analysis software). The total counts of 30 neurons/mm2 were compared between the GPE and vehicle treated group. The data has not been analysed for statistical signigicance. Tissue from one of the GPE treated animals was unable to be counted. These results are presented in Figures 4-6.

Printed from Mimosa Results Figure 4 shows a loss of GAD immumoreactivity in the striatum on both the left (non-mjured) and right sides of the brain following injury. GPE induced an upregulation of GAD expression on both sides of the brain.

Figure 5 shows a loss of NOS activity m the striatum after injury on both the left and right sides of the brain. GPE induced an upregulation of enzyme expression on both sides of the brain, restoring the enzyme levels to normal on the left (non-mjured) side of the brain.

Figure 6 shows a loss of ChAT immunoreactivity after injury on both sides of the brain. GPE treatment upregulated the levels of ChAT to above normal on the right (injured) side of the brain.

Conclusions These results demonstrate the ability of GPE to regulate the expression of GAD, NOS and ChAT in the CNS.

Furthermore, the results indicate that GAD and NOS are regulated in the presence and absence of the quinolinic acid induced injury. This clearly demonstrates the effect of GPE m upregulating GAD and NOS expression independent of a response to neural damage or a threat to neural cell survival.

The results also show that ChAT can be upregulated by GPE in the presence of quinotmic acid mduced neural injury.

INDUSTRIAL APPLICATION The experimental results demonstrate the ability of GPE to increase the amount of the neural enzymes ChAT, GAD and NOS m the CNS through a direct increase in enzyme expression. Further, the results indicate that expression of both ChAT and NOS is upregulated both in the presence and absence of neural injury. This clearly represents Printed from Mimosa that the effect of GPE In upregulating expression of these enzymes is independent of a response to neural damage or a threat to neural cell survival.

These findings make GPE and its analogs applicable in treating a number of 5 neurological disorders or conditions, either therapeutically or prophylactically. Indeed, it will be apparent to those persons skilled in the art that GPE and its analogs can be employed at any time where a patient would benefit from an increase in the expression of ChAT, GAD or NOS within the CNS. Neurological disorders or conditions which would benefit from this include, but are not limited to the following: motor neuron disease, Alzheimers disease, muscular dystrophy, peripheral neuropathies, autonomic neuropathies, memory loss, aging and other forms of neurodegeneration (ChAT); postasphyxial seizures, epilepsy and other convulsive disorders, neurodegenerative diseases such as Huntingtons, plus the immediate post acute phase following head trauma, stroke, and other forms of hypoxic ischemic brain injury (GAD); and subarachonoid haemorrahge, transient ischemic attack, stroke, multinfarct dementia, cerebral vasculitis and traumatic brain injury plus the immediate post acute phase following head trauma, stroke and other forms of hypoxic ischemic brain injury.

It will be appreciated that although the present invention is described above with reference to certain specific embodiments, the description provided is exemplary only and that the invention is limited only by the lawful scope of the appended claims.

Printed from Mimosa

Claims (17)

CLAIMS:

1. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof in the preparation of a medicament for administration to a patient, in order to cause a desired increase in at least one neural enzyme selected from the range of ChAT, GAD, or NOD for therapeutic or prophylactic treatment of an appropriate neurological condition.

2. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof in the preparation of a medicament for administration to a patient, in order to cause upregulation of expression of at least one neural enzyme selected from the range of ChAT, GAD, or NOD for therapeutic or prophylactic prophylactic treatment of an appropriate neurological condition.

3. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof according to either claim 1 or claim 2 wherein the condition is one in which an increase in the amount of ChAT is desirable.

4. Use as claimed in claim 3, wherein the condition is selected from Motor Neuron disease, Alzheimer's disease, muscular dystrophy, peripheral neuropathies, autonomic neuropathies, memory loss, and neurodegeneration due to aging.

5. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof according to either claim 1 or claim 2 wherein the condition is one in which an increase in the amount of GAD is desirable.

6. Use as claimed in claim 5, wherein the condition is selected from post-asphyxial seizures, convulsive disorders, neurodegenerative disease, and hypoxic-ischemic brain injury.

7. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof according to either claim 1 or claim 2 wherein the condition is one in which an increase in the amount of NOS is desirable.

8. Use as claimed in claim 7, wherein the condition is selected from subarachnoid hemorrhage, transient ischemic attack, stroke, multi-infarct dementia, cerebral vasculitis, and traumatic brain injury. \S INTELLECTUAL PROPERTY OFFICE OF NZ. 1 k FEB 2001 RECEIVED

9. Use of GPE or an analogue thereof or a prodrug thereof in the preparation of a medicament for administration to a patient for therapeutic or prophylactic treatment of an appropriate neurological condition, where the amount contained in the medicament to be administered is in the range of from 0.04 micrograms to 1000 micrograms of GPE per 100 grams of body weight.

10. Use of GPE or an analogue thereof or a prodrug thereof in the preparation of a medicament for administration to a patient for therapeutic or prophylactic treatment of an appropriate neurological condition, where the amount contained in the medicament to be administered is about 3 micrograms GPE per 100 grams of body weight.

11. Use of an effective amount of GPE or an analogue thereof or a prodrug thereof and a vehicle including an about 0.1M citrate buffer at a pH of about 6, in the preparation of a medicament for systemic administration to a patient requiring therapeutic or prophylactic treatment of at least one neurological condition.

12. A method of treatment of a non-human animal suffering from or susceptible to a condition in which an increase in the amount of at least one neural enzyme selected from the range of ChAT, GAD, or NOD is desirable, which method comprises the step of increasing the effective amount of GPE or an analogue thereof or a prodrug thereof within the CNS of said non-human animal.

13. A method of treatment of a non-human animal as claimed in claim 12, wherein the concentration of GPE or an analogue thereof is increased by administering to said non-human animal an effective amount of GPE or an analogue thereof or a prodrug thereof.

14.A method of treatment as claimed in claim 13, wherein the concentration of GPE is increased in the CNS by direct administration of GPE.

15. A method of treatment according to any one of claims 12 to 14, which method is prophylactic.

16. A method of treatment according to any one of claims 12 to 14, which method is therapeutic. , A INTELLECTUAL PROPERTY 10 OFFICE OF NZ. 1 4 FEB 2001 ocrciucn cr~? r 7 " r "3 £ T7 w /n /. / '

17. A method of prophylaxis or treatment of a non-human animal for a neurological condition, said method involving upregulation of the expression of at least one neural enzyme selected from the range of ChAT, GAD, or NOD,- the method of treatment being substantially that described in any one of the examples given in the accompanying specification and with or without reference to the accompanying drawings. Ensor and Associates for the Applicants, Neuronz Limited. END OF CLAIMS INTELLECTUAL PROPERTY OFFICE OF N Z. M 1 4 FEB 2001 RECEIVED