NZ334103A - an expression vector used to transform or transfect a host cell to make a thrombopoietin polypeptide, the cultured yeast cell thereof and the thrombopoietin polypeptide - Google Patents

an expression vector used to transform or transfect a host cell to make a thrombopoietin polypeptide, the cultured yeast cell thereof and the thrombopoietin polypeptide

Info

Publication number
NZ334103A
NZ334103A NZ334103A NZ33410397A NZ334103A NZ 334103 A NZ334103 A NZ 334103A NZ 334103 A NZ334103 A NZ 334103A NZ 33410397 A NZ33410397 A NZ 33410397A NZ 334103 A NZ334103 A NZ 334103A
Authority
NZ
New Zealand
Prior art keywords
amino acid
polypeptide
acid residues
thrombopoietin
residues
Prior art date
Application number
NZ334103A
Inventor
Meher Irani
Gary R Morrison-Nelson
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of NZ334103A publication Critical patent/NZ334103A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/524—Thrombopoietin, i.e. C-MPL ligand
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The expression vector replicable in a eukaryotic host cell comprises: a) a transcription promoter; b) a first DNA segment encoding a secretory leader; c) a second DNA segment encoding a thrombopoietin polypeptide backbone consisting of C-X-B, wherein C is a human thrombopoietin cytokine domain polypeptide; X is a peptide bond or a linker consisting of one or two amino acid residues, subject to the limitation that X, alone or in combination with C or B, does not provide a dibasic amino acid pair; and B is a polypeptide consisting of residues 1 to y of SEQ ID NO:3, wherein y is an integer from 5 to 18, and wherein up to 35 % of the amino acid residues of B are individually replaced by other amino acid residues and d) a transcription terminator. The polypeptides are characterized by an amino acid backbone consisting of C-X-B wherein: C is a human thrombopoietin cytokine domain polypeptide; X is a peptide bond or a linker consisting of one or two amino acid residues, subject to the limitation that X, alone or in combination with C or B, does not provide a dibasic amino acid pair; and B is a polypeptide consisting of residues 1 to y of SEQ ID NO:3, wherein y is an integer from 5 to 18, and wherein up to 35 % of the amino acid residues of B are individually replaced by other amino acid residues. The polypeptides are useful with methods of stimulating proliferation or development of hematopoietic cells in vitro and in vivo.
NZ334103A 1996-08-13 1997-07-30 an expression vector used to transform or transfect a host cell to make a thrombopoietin polypeptide, the cultured yeast cell thereof and the thrombopoietin polypeptide NZ334103A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US69644796A 1996-08-13 1996-08-13

Publications (1)

Publication Number Publication Date
NZ334103A true NZ334103A (en) 2000-09-29

Family

ID=24797104

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ334103A NZ334103A (en) 1996-08-13 1997-07-30 an expression vector used to transform or transfect a host cell to make a thrombopoietin polypeptide, the cultured yeast cell thereof and the thrombopoietin polypeptide

Country Status (8)

Country Link
EP (1) EP0920511A1 (en)
JP (1) JP2000516465A (en)
KR (1) KR20000029998A (en)
CN (1) CN1230993A (en)
AU (1) AU728881B2 (en)
CA (1) CA2262507A1 (en)
NZ (1) NZ334103A (en)
WO (1) WO1998006849A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU750803B2 (en) 1997-10-29 2002-07-25 University Of Pittsburgh Use of vectors such as adenoviruses and/or adeno associated viruses and/or retroviruses and/or herpes simplex viruses and/or liposomes and/or plasmids as a vehicle for genetic information enabling mammal cells to produce agents for the treatment of bone pathologies
KR19990081421A (en) * 1998-04-29 1999-11-15 성재갑 Method for producing human platelet promoter (TPO) using animal cells
WO2002015926A1 (en) * 2000-08-24 2002-02-28 Kirin Beer Kabushiki Kaisha c-mpl LIGAND-CONTAINING MEDICINAL COMPOSITIONS FOR INCREASING PLATELETS AND ERYTHROCYTES
WO2004078780A1 (en) * 2003-03-04 2004-09-16 Pepharm R&D Limited Pharmaceutical composition containing l-seryl-l-leucine

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2285446B (en) * 1994-01-03 1999-07-28 Genentech Inc Thrombopoietin
EP0668352A1 (en) * 1994-02-14 1995-08-23 Kirin Brewery Company, Ltd. Protein having TPO activity

Also Published As

Publication number Publication date
JP2000516465A (en) 2000-12-12
EP0920511A1 (en) 1999-06-09
AU728881B2 (en) 2001-01-18
WO1998006849A1 (en) 1998-02-19
CN1230993A (en) 1999-10-06
AU3823897A (en) 1998-03-06
KR20000029998A (en) 2000-05-25
CA2262507A1 (en) 1998-02-19

Similar Documents

Publication Publication Date Title
GR3018123T3 (en) Expression of the recombinant tumor necrosis factor binding protein I (TBP-I).
NO20032250D0 (en) Use of a hpG-CSF polypeptide for the preparation of a drug for arresting the proliferation of leukemia cells
ES8307297A1 (en) Polypeptides, process for their microbial production, intermediates therefor and compositions containing them.
EP0232112A3 (en) Recombinant protein complex having human factor viii:c activity its production and use
WO1998022574A3 (en) Type i recombinant clostridium histolyticum collagenase and its use for isolating cells and cell aggregates
ATE49233T1 (en) YEAST TRANSFORMATION VECTORS.
ATE82321T1 (en) CDNA CLONES ENCODING PEPTIDES WITH HUMAN GRANULOCYTE, MACROPHAGE AND EOSINOPHIL CELL GROWTH FACTOR ACTIVITIES.
DE69426289D1 (en) Signal sequences for the secretion of heterologous proteins from yeast
IE871871L (en) Cloning and expression of acidic fibroblast growth factor
NZ334103A (en) an expression vector used to transform or transfect a host cell to make a thrombopoietin polypeptide, the cultured yeast cell thereof and the thrombopoietin polypeptide
Holter et al. Efficient gene transfer by sequential treatment of mammalian cells with DEAE-dextran and deoxyribonucleic acid
ATE79409T1 (en) MANUFACTURING PROCESS FOR HUMAN ATRIAL NATRIURETIC POLYPEPTIDE.
NZ500844A (en) Nucleotide sequences (mutated GDF-9B polypeptides) involved in increasing and decreasing mammalian ovulation rate
DE59712567D1 (en) Chemokine vom cc-typ
GB9915200D0 (en) Neurotrophic factor receptor
KR940007182A (en) Recombinant human alpha interferon expression vector, strain and method for producing human alpha interferon using the same
NZ536319A (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same

Legal Events

Date Code Title Description
RENW Renewal (renewal fees accepted)
RENW Renewal (renewal fees accepted)