NL2031584B1 - Use of composition containing fermented eucalyptus product in inhibiting weeds - Google Patents
Use of composition containing fermented eucalyptus product in inhibiting weeds Download PDFInfo
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- NL2031584B1 NL2031584B1 NL2031584A NL2031584A NL2031584B1 NL 2031584 B1 NL2031584 B1 NL 2031584B1 NL 2031584 A NL2031584 A NL 2031584A NL 2031584 A NL2031584 A NL 2031584A NL 2031584 B1 NL2031584 B1 NL 2031584B1
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- eucalyptus
- fermented
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- weeds
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- 239000000203 mixture Substances 0.000 title claims abstract description 94
- 241000196324 Embryophyta Species 0.000 title claims abstract description 65
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 11
- 244000166124 Eucalyptus globulus Species 0.000 title abstract 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 241000219927 Eucalyptus Species 0.000 claims description 130
- NGJANBBWZSMYRM-UHFFFAOYSA-N 2,4,6-Trihydroxy-3-methyl-5-(3-methylbutanoyl)benzaldehyde Chemical compound CC(C)CC(=O)C1=C(O)C(C)=C(O)C(C=O)=C1O NGJANBBWZSMYRM-UHFFFAOYSA-N 0.000 claims description 39
- 239000000463 material Substances 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 28
- 241000186046 Actinomyces Species 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 235000015639 rosmarinus officinalis Nutrition 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 14
- 238000010564 aerobic fermentation Methods 0.000 claims description 11
- 230000008635 plant growth Effects 0.000 claims description 7
- 241000235644 Issatchenkia Species 0.000 claims description 4
- 239000003112 inhibitor Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 3
- 230000001332 colony forming effect Effects 0.000 claims description 2
- 241000557821 Azadirachta Species 0.000 claims 2
- 241001530490 Salvia rosmarinus Species 0.000 claims 2
- 230000035784 germination Effects 0.000 abstract description 19
- 230000012010 growth Effects 0.000 abstract description 13
- 239000002689 soil Substances 0.000 abstract description 12
- 230000018109 developmental process Effects 0.000 abstract description 10
- 239000002245 particle Substances 0.000 abstract description 10
- 239000003895 organic fertilizer Substances 0.000 abstract description 5
- 239000000758 substrate Substances 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 3
- 239000011550 stock solution Substances 0.000 description 16
- 244000237986 Melia azadirachta Species 0.000 description 15
- 235000013500 Melia azadirachta Nutrition 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- 239000000126 substance Substances 0.000 description 14
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 13
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 13
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 13
- 244000178231 Rosmarinus officinalis Species 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 6
- 230000035764 nutrition Effects 0.000 description 6
- 238000009331 sowing Methods 0.000 description 6
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 244000101284 Pinus kesiya Species 0.000 description 5
- 235000005026 Pinus kesiya Nutrition 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 229910001425 magnesium ion Inorganic materials 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000003415 peat Substances 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011591 potassium Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 241000906682 Hemsleya Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 4
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 3
- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 3
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 3
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 3
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 3
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 229910001710 laterite Inorganic materials 0.000 description 2
- 239000011504 laterite Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000008121 plant development Effects 0.000 description 2
- 235000010333 potassium nitrate Nutrition 0.000 description 2
- 239000004323 potassium nitrate Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000001331 rosmarinus officinalis leaf Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241000234646 Cyperaceae Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241001107098 Rubiaceae Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002373 plant growth inhibitor Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P13/00—Herbicides; Algicides
- A01P13/02—Herbicides; Algicides selective
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The disclosure belongs to the technical field of agricultural and forestry seedling raising. The disclosure specifically relates to use of a composition containing a fermented eucalyptus product in inhibiting weeds. In the disclosure, the composition containing a fermented eucalyptus product is prepared by a specific preparation method, which ensures that the composition containing a fermented eucalyptus product has a suitable particle porosity and an air permeability, and is rich in organic matters, and Grand/no! in the composition has a concentration of 200 mg/kg to 1,000 mg/kg. The composition integrates advantages of an organic seedling substrate and a bio-organic fertilizer, and could ensure normal growth and development of seedlings. In addition, the composition effectively inhibits germination of weed seeds in soil to reduce an incidence of the weeds by 29.41 % to 83.45%.
Description
USE OF COMPOSITION CONTAINING FERMENTED EUCALYPTUS
PRODUCT IN INHIBITING WEEDS
[0001] The present disclosure belongs to the technical field of agricultural and forestry seedling raising. The present disclosure specifically relates to use of a composition containing a fermented eucalyptus product in inhibiting weeds.
[0002] Weeds are one of the important factors affecting plant germination and growth. Weed species represented by Gramineae, Cyperaceae,
Compositae, Solanaceae, Amaranthaceae and Rubiaceae grow rapidly and compete with crops and forest tree seedlings for water, fertilizer, light and space. If not dealt with in time, the phenomenon of "weeds covering seedlings" is particularly likely to occur in rainy seasons, affecting growth and development of the seedlings and reducing a survival rate and quality of the seedlings.
[0003] Although having a desirable removal effect on the weeds, chemical herbicides may induce the generation of drug-resistant weeds after a long-term use, increasing the difficulty of weed control. Manual weeding requires a lot of labour, which increases labour costs as well as easily damages root systems of the seedlings. It is a technical problem to be solved urgently during seedling raising in agriculture and forestry by seeking a method to effectively inhibit the germination of weed seeds in the soil and reduce the incidence of weeds, while ensuring normal growth and development of the crops and forest tree seedlings.
[0004] An object of the present disclosure is to provide use of a composition containing a fermented eucalyptus product in inhibiting weeds. The composition could effectively inhibit germination of weed seeds in soil while ensuring normal growth and development of seedlings.
[0005] To achieve the above object, the present disclosure provides the following technical solutions.
[0006] The present disclosure provides use of a composition containing a fermented eucalyptus product in inhibiting weeds, where Grandinol (CAS: 63861-11-0) in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg.
[0007] The present disclosure further provides use of a composition containing a fermented eucalyptus product in preparation of a weed inhibitor, where Grandino! in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg.
[0008] The present disclosure further provides use of a composition containing a fermented eucalyptus product in plant growth and development, where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg.
[0009] In some embodiments, the composition containing a fermented eucalyptus product is prepared by a method including the following steps:
[0010] conducting aerobic fermentation on an eucalyptus material by mixed bacteria to obtain the fermented eucalyptus product; and
[0011] mixing the fermented eucalyptus product, Azadirachta indica seeds and Rosmarinus officinalis leaves to obtain the composition containing a fermented eucalyptus product,
[0012] where the mixed bacteria comprise /ssatchenkia, Bacillus subtilis and Actinomyces.
[0013] In some embodiments, a volume ratio of the /ssatchenkia, the
Bacillus subtilis and the Actinomyces is in the range of (7.4-7.6) : (1.9- 2.1): (0.4-0.6).
[0014] In some embodiments, the /ssatchenkia may have a colony forming unit (CFU) of 80 million/ml to 100 million/ml; the Bacillus subtilis may have a CFU of 80 million/ml to 100 million/ml; and the Actinomyces may have a CFU of 80 million/ml to 100 million/ml.
[0015] In some embodiments, during the aerobic fermentation, a ratio of the mass of the eucalyptus material to the volume of the mixed bacteria is in the range of 250 g: (7-8) ml.
[0016] In some embodiments, the aerobic fermentation is conducted for 30 d to 40d.
[0017] In some embodiments, a mass ratio of the fermented eucalyptus product, the Azadirachta indica seeds and the Rosmarinus officinalis leaves is in the range of (98.5-99.2) : (0.3-0.5) : (0.5-1).
[0018] The present disclosure further provides a method for promoting plant growth, including cultivating a plant using the composition containing a fermented eucalyptus product, where Grandino/ in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg.
[0019] The present disclosure provides use of a composition containing a fermented eucalyptus product in inhibiting weeds, where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg. The composition containing a fermented eucalyptus product is used during the growth and development of crops and forest tree seedlings, which has advantages of an organic seedling substrate and a bio-organic fertilizer, ensuring normal growth and development of seedlings, and effectively inhibiting germination of weed seeds in soil. Results of examples of the present disclosure show that the composition containing a fermented eucalyptus product reduces an incidence of the weeds by 29.41% to 83.45%.
[0020] In the present disclosure, the method for preparing the composition containing a fermented eucalyptus product is defined to ensure that the composition containing a fermented eucalyptus product has a suitable particle porosity and an air permeability, and is rich in organic matters, thereby providing abundant nutrients for rhizosphere microorganisms, which could significantly shorten adaptation period, promote robust growth of plants, and reduce use of chemical fertilizers and pesticides.
[0021] The present disclosure provides use of a composition containing a fermented eucalyptus product in inhibiting weeds, where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg.
[0022] In the present disclosure, Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg, preferably 200 mg/kg to 400 mg/kg, 450 mg/kg to 700 mg/kg, or 750 mg/kg to 1,000 mg/kg, more preferably 250 mg/kg to 350 mg/kg, 500 mg/kg to 650 mg/kg, or 800 mg/kg to 900 mg/kg. In the present disclosure, Grandinol is a potent plant growth inhibitor, which is controlled to have a concentration of 200 mg/kg to 1,000 mg/kg in the composition containing a fermented eucalyptus product, and is used to prepare the composition containing a fermented eucalyptus product, which could effectively inhibit the germination of weed seeds in soil.
[0023] In some embodiments, the composition containing a fermented eucalyptus product is prepared by a method including the following steps:
[0024] conducting aerobic fermentation on an eucalyptus material by mixed bacteria to obtain the fermented eucalyptus product; and
[0025] mixing the fermented eucalyptus product, Azadirachta indica seeds and Rosmarinus officinalis leaves to obtain the composition containing a fermented eucalyptus product,
[0026] where the mixed bacteria comprise /ssatchenkia, Bacillus subtilis and Actinomyces.
[0027] In the present disclosure, the aerobic fermentation is conducted on the eucalyptus material by the mixed bacteria to obtain the fermented eucalyptus product. The ratio of the mass of the eucalyptus material to the volume of the mixed bacteria is in the range of 250 g: (7-8) ml. In some embodiments, the eucalyptus material is a wood raw material before or after processing of eucalyptus, preferably including one or more selected from the group consisting of bark, sawdust, shavings, broken wood pieces and wood chips, and more preferably the bark.
[0028] In some embodiments, the mixed bacteria comprise /ssatchenkia,
Bacillus subtilis and Actinomyces. In some embodiments, the bacteria are mixed in the form of bacterial stock solutions. In some embodiments, a volume ratio of the /ssatchenkia stock solution, the Bacillus subtilis stock solution and the Actinomyces stock solution is in the range of (7.4-7.6) : (1.9-2.1) : (0.4-0.6), preferably 7.5 : 2 : 0.5. In some embodiments, the
Issatchenkia stock solution has a CFU of 80 million/ml to 100 million/ml, preferably 80 million/ml to 90 million/ml. In some embodiments, the
Bacillus subtilis stock solution has a CFU of 80 million/ml to 100 million/ml, preferably 80 million/ml to 90 million/ml. In some embodiments, the Actinomyces stock solution has a CFU of 80 million/ml to 100 million/ml, preferably 80 million/ml to 90 million/ml.
[0029] The /ssatchenkia stock solution, the Bacillus subtilis stock solution and the Actinomyces stock solution of the present disclosure are prepared by adding corresponding microorganisms to culture solutions respectively. The culture solutions are not limited to the formula disclosed in the present disclosure, and other culture solutions which are able to achieve similar effects may also be used. In an example of the present disclosure, culture solutions for culturing the /ssatchenkia, the Bacillus subtilis and the Actinomyces each include the following components by mass percentage: 3% of sucrose, 0.1% of potassium nitrate, 0.01% of sodium chloride, 0.012% of magnesium sulphate, and a balance of water.
[0030] In some embodiments of the present disclosure, during the aerobic fermentation, the eucalyptus material is pulverized into a fibrous shape with a length of 0.2 cm to 2.5 cm, and then mixed with the mixed bacteria evenly. In some embodiments, the aerobic fermentation is conducted at ambient temperature for 30 days to 40 days, preferably 35 days to 40 days. There is no strict requirement for a specific method of the aerobic fermentation, as long as the eucalyptus material and the mixed bacteria could be piled up and then fermented naturally at ambient temperature.
[0031] In the present disclosure, after the fermented eucalyptus product is obtained, the fermented eucalyptus product, Azadirachta indica seeds and Rosmarinus officinalis leaves are mixed to obtain the composition containing a fermented eucalyptus product.
[0032] In some embodiments of the present disclosure, the mass ratio of the fermented eucalyptus product, the Azadirachta indica seeds and the
Rosmarinus officinalis leaves is in the range of (98.5-99.2) : (0.3-0.5) : (0.5-1), preferably (98.8-99.0) : (0.35-0.45) : (0.6-0.8). In some embodiments, the Azadirachta indica seeds are in the form of granular, with a granularity of less than 1 cm. In some embodiments, fresh
Azadirachta indica seeds are dried to have a moisture content of 20% to 25%, and the dried Azadirachta indica seeds are pulverized to obtain granular Azadirachta indica seeds. There is no strict requirement on a method of the pulverization, and the pulverization may be conducted routinely. There is no strict requirement on a method of drying the
Azadirachta indica seeds, and the Azadirachta indica seeds may be air- dried, shade-dried or dried at a temperature of not greater than 65 °C.
[0033] In the present disclosure, in some embodiments, the Rosmarinus officinalis leaves are in the form of granular, with a granularity of not more than 1 cm. In some embodiments, fresh Rosmarinus officinalis leaves are dried to have a moisture content of 20% to 25%, and the dried
Rosmarinus officinalis leaves are pulverized to obtain granular
Rosmarinus officinalis leaves. There is no strict requirement on a method of the pulverization, and the pulverization may be conducted routinely.
There is no strict requirement on a method of drying the Rosmarinus officinalis leaves, and the Rosmarinus officinalis leaves may be air-dried, shade-dried or dried at a temperature of not greater than 65°C.
[0034] In the present disclosure, the composition containing a fermented eucalyptus product is prepared by the method above, which ensures the particle porosity and air permeability of the composition containing a fermented eucalyptus product, and enables the root system of the plant seedlings to be grown and intertwined with the organic fertilizer particles in the bark to form a root mass, such that it is not easy to disperse during transportation and transplanting. In addition, the composition containing a fermented eucalyptus product has sufficiently degraded inhibitors, complete nutrient release, similar physical and chemical properties to peat soil, and organic matter and nutrient contents not lower than the peat soil, with an excellent fertility. The composition could replace the traditional non-renewable resource peat soil as an organic fertilizer suitable for the application of economical forest trees, commercial crops and flower medicinal materials, which is convenient for transportation and reduces costs. During the growth and development of crops and forest tree seedlings, the composition containing a fermented eucalyptus product could provide abundant nutrients for rhizosphere microorganisms, significantly shorten a slow seedling period, promote robust growth of plants, and reduce use of chemical fertilizers and pesticides. The composition containing a fermented eucalyptus product has advantages of an organic seedling substrate and a bio-organic fertilizer, ensuring normal growth and development of seedlings, and effectively inhibiting germination of weed seeds in soil. Results of examples of the present disclosure show that the composition containing a fermented eucalyptus product reduces the incidence of the weeds by 29.41% to 83.45%.
[0035] The present disclosure further provides use of a composition containing a fermented eucalyptus product in the preparation of a weed inhibitor, where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg, preferably 200 mg/kg to 400 mg/kg, or 450 mg/kg to 700 mg/kg, or 750 mg/kg to 1,000 mg/kg, more preferably 250 mg/kg to 350 mg/kg, or 500 mg/kg to 650 mg/kg, or 800 mg/kg to 900 mg/kg. In the present disclosure, a method for preparing the composition containing a fermented eucalyptus product is the same as the above method, and will not be repeated here.
[0036] The present disclosure further provides use of a composition containing a fermented eucalyptus product in plant growth and development, where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg, preferably 200 mg/kg to 400 mg/kg, or 450 mg/kg to 700 mg/kg, or 750 mg/kg to 1,000 mg/kg, more preferably 250 mg/kg to 350 mg/kg, or 500 mg/kg to 650 mg/kg, or 800 mg/kg to 900 mg/kg. In the present disclosure, a method for preparing the composition containing a fermented eucalyptus product is the same as the above method, and will not be repeated here.
[0037] The present disclosure further provides a method for promoting plant growth, including cultivating a plant using the composition containing a fermented eucalyptus product; where Grandinol in the composition containing a fermented eucalyptus product has a concentration of 200 mg/kg to 1,000 mg/kg, preferably 200 mg/kg to 400 mg/kg, or 450 mg/kg to 700 mg/kg, or 750 mg/kg to 1,000 mg/kg, more preferably 250 mg/kg to 350 mg/kg, or 500 mg/kg to 650 mg/kg, or 800 mg/kg to 900 mg/kg. In some embodiments, the plant includes a crop or a forest tree. In some embodiments, the crop includes an economic crop, preferably broccoli or a Chinese herbal medicine. In some embodiments, the forest tree includes an arbour, preferably Pinus kesiya.
[0038] The technical solutions provided by the present disclosure are described in detail below with reference to the examples, but the examples cannot be understood as limiting the protection scope of the present disclosure.
[0039] Example 1
[0040] A composition containing a fermented eucalyptus product was prepared as follows:
[0041] 1. A medium was prepared according to the following components by mass percentage:
[0042] 4.5% of sucrose, 0.11% of potassium nitrate, 0.01% of sodium chloride, 0.014% of magnesium sulphate, and 95.366% of water.
[0043] 2. /ssatchenkia, Bacillus subtilis and Actinomyces were cultured to have a CFU of 80 million/ml in the medium prepared in step 1 to obtain an /ssatchenkia stock solution, a Bacillus subtilis stock solution, and an
Actinomyces stock solution, respectively. Then, the /ssatchenkia stock solution, the Bacillus subtilis stock solution, and the Actinomyces stock solution were mixed according to a volume ratio of 7.4 : 21 : 0.5, to obtain a mixed bacterial solution.
[0044] 3. An eucalyptus bark was pulverized into fibres, with a length of 2 cm, and the mixed bacterial solution prepared in step 2 was added for mixing uniformly to obtain an eucalyptus material, where 28 L of the mixed bacterial solution was added in per ton of the eucalyptus material.
[0045] 4. The eucalyptus material containing the mixed bacterial solution obtained in step 3 was piled for natural fermentation for 40 d at ambient temperature, such that Grandinol had a content of 210 mg/kg in the eucalyptus material, to obtain a fermented and decomposed eucalyptus material.
[0046] 5. Fresh Azadirachta indica seeds were dried at 55 °C to have a moisture content of 20%, and then the dried Azadirachta indica seeds were pulverized to have a particle size of 0.5 cm to obtain Azadirachta indica seed particles.
[0047] 6. Fresh Rosmarinus officinalis leaves were dried at 55 °C to have a moisture content of 20%, and then the dried Rosmarinus officinalis leaves were pulverized to have a particle size of 0.5 cm to obtain
Rosmarinus officinalis leaf particles.
[0048] 7. In percentages by weight, 98.5% of the fermented and decomposed eucalyptus material obtained in step 4, 0.5% of the
Azadirachta indica seed particles obtained in step 5, and 1% of the
Rosmarinus officinalis leaf particles obtained in step 6 were stirred and mixed uniformly to obtain the composition containing a fermented eucalyptus product.
[0049] Physical and chemical indicators of the composition containing a fermented eucalyptus product were measured, and measurement results are shown in Table 1.
[0050] Table 1 Physical and chemical indicators of the composition containing a fermented eucalyptus product
[0051]
PH
Available Fe ion me
Available Mg ion 78 mm ees |"
ToslHg | mgkg | 006
[0052] As can be seen from Table 1, after fermentation and decomposition, the nutrients in the eucalyptus material are released, and the composition containing a fermented eucalyptus product has high contents of organic matters, nitrogen, phosphorus and potassium.
[0053] Example 2
[0054] Example 2 was conducted according to the method of Example 1, except that, in step 4: the eucalyptus material containing the mixed bacterial solution obtained in step 3 was piled for natural fermentation for 35 days at ambient temperature, such that Grandinol had a content of 530 mg/kg in the eucalyptus material, to obtain a fermented and decomposed eucalyptus material.
[0055] Physical and chemical indicators of the composition containing a fermented eucalyptus product were measured, and the results are shown in Table 2.
[0056] Table 2 Physical and chemical indicators of the composition containing a fermented eucalyptus product
[0057] eA 82
Available Fe ion 210
IT ewe
Available Mg ion 42 ewe 7
[0058] As can be seen from Table 2, after fermentation and decomposition, the nutrients in the eucalyptus material are released, and the composition containing a fermented eucalyptus product has relatively high contents of organic matters, nitrogen, phosphorus and potassium.
[0059] Example 3
[0060] Example 3 was conducted according to the method of Example 1, except that, in step 4: the eucalyptus material containing the mixed bacterial solution obtained in step 3 was piled for natural fermentation for 30 days at ambient temperature, such that Grandino/ had a content of 980 mg/kg in the eucalyptus material, to obtain a fermented and decomposed eucalyptus material.
[0061] Physical and chemical indicators of the composition containing a fermented eucalyptus product were measured, and the results are shown in Table 3.
[0062] Table 3 Physical and chemical indicators of the composition containing a fermented eucalyptus product
[0063]
PH
Available Fe ion 130 ae eee
Available Mg ion 21
Tome
[0064] As can be seen from Table 3, after fermentation and decomposition, nutrients such as organic matters, nitrogen, phosphorus and potassium in the eucalyptus material can be released, and the composition containing a fermented eucalyptus product has a reduced amount of the released nutrients compared to Example 1 and Example 2.
[0065] Comparative Example 1
[0066] Comparative example 1 was conducted according to the method of Example 1, except that, in step 4: the eucalyptus material containing the mixed bacterial solution obtained in step 3 was piled for natural fermentation for 45 days at ambient temperature, such that Grandino/ had a content of 150 mg/kg in the eucalyptus material, to obtain a fermented and decomposed eucalyptus material.
[0067] Physical and chemical indicators of the composition containing a fermented eucalyptus product were measured, and the results are shown in Table 4.
[0068] Table 4 Physical and chemical indicators of the composition containing a fermented eucalyptus product
[0069] pH 70 _15mmparticlesize | % | eo
Available Fe ion
Tome
Available Mg ion 83 ewe 0
ToslHg | mgkg | 006
[0070] As can be seen from Table 4, after fermentation and decomposition, the nutrients in the eucalyptus material are released. The composition containing a fermented eucalyptus product has high contents of organic matters, nitrogen, phosphorus and potassium, while has a concentration of Grandino/ of 150 mg/kg.
[0071] Comparative Example 2
[0072] Comparative example 2 was conducted according to the method of Example 1, except that, in step 4: the eucalyptus material containing the mixed bacterial solution obtained in step 3 was piled for natural fermentation for 25 days at ambient temperature, such that Grandinol had a content of 1,100 mg/kg in the eucalyptus material, to obtain a fermented and decomposed eucalyptus material.
[0073] Physical and chemical indicators of the composition containing a fermented eucalyptus product were measured, and the results are shown in Table 5.
[0074] Table 5 Physical and chemical indicators of the composition containing a fermented eucalyptus product
[0075] m8
Available Fe ion 105
Tome
Available Mg ion 11 me
[0076] As can be seen from Table 5, after fermentation and decomposition, the nutrients in the eucalyptus material are released. The composition containing a fermented eucalyptus product has relatively low contents of organic matters, nitrogen, phosphorus and potassium, and a relatively high concentration of Grandinol.
[0077] Use Example 1-1
[0078] In a nursery greenhouse, floating seedlings of a vegetable, broccoli was conducted using the composition containing a fermented eucalyptus product obtained in Example 1. The nursery greenhouse had a daily maximum temperature of 41 °C, a minimum temperature of 9 °C, an average temperature of 25.8 °C, an average relative humidity of 64%, and an average sunshine duration of 11.4 h.
[0079] Specifically, on March 18, 2019, the composition containing a fermented eucalyptus product obtained in Example 1 was filled with all holes of 10 floating seedling trays with a size of 162 holes, where 1 seed was sown in each hole. After 15 days of sowing, broccoli seeds had a germination rate of 74.1%; and after 40 days of broccoli in the seedling period, each seedling tray had 1-4 weeds, with the incidence of weeds of 0.62% to 2.47%.
[0080] Use Example 1-2
[0081] Use example 1-2 was conducted according to the method of Use
Example 1-1, except that: commercially available peat soil was filled with all holes of 10 floating seedling trays with a size of 162 holes, where 1 seed was sown in each hole. After 15 days of sowing, broccoli seeds had a germination rate of 62.5%; and after 40 days of broccoli in the seedling period, each seedling tray had 6-8 weeds, with the incidence of weeds of 3.70% to 4.94%.
[0082] Use Example 1-3
[0083] Use example 1-3 was conducted according to the method of Use
Example 1-1, except that: the composition containing a fermented eucalyptus product obtained in Comparative Example 1 was filled with all holes of 10 floating seedling trays with a size of 162 holes, where 1 seed was sown in each hole. After 15 days of sowing, broccoli seeds had a germination rate of 72.8%; and after 40 days of broccoli in the seedling period, each seedling tray had 8-12 weeds, with the incidence of weeds of 4.94% to 7.41%.
[0084] Use Example 1-4
[0085] Use example 1-4 was conducted according to the method of Use
Example 1-1, except that: the composition containing a fermented eucalyptus product obtained in Comparative Example 2 was filled with all holes of 10 floating seedling trays with a size of 162 holes, where 1 seed was sown in each hole. After 15 days of sowing, broccoli seeds had a germination rate of 13.0%; and after 40 days of broccoli in the seedling period, each seedling tray had 1-4 weeds, with the incidence of weeds of 0.62% to 2.47%.
[0086] By comparing the occurrence of weeds in Use Example 1-1, Use
Example 1-2, Use Example 1-3 and Use Example 1-4, it can be seen that compared with commercially available peat soil, the composition containing a fermented eucalyptus product obtained in Example 1 can significantly reduce the occurrence of weeds and reduce the incidence of weeds by 33.24% to 87.45% under the premise of ensuring the normal germination of broccoli seeds; the composition containing a fermented eucalyptus product obtained in Comparative Example 1 can guarantee the germination of broccoli seeds, but cannot inhibit the growth of weeds; and the composition containing a fermented eucalyptus product obtained in Comparative Example 2 can inhibit the occurrence of weeds, but significantly reduces the germination rate of broccoli seeds.
[0087] Use Example 2-1
[0088] Seedlings of Pinus kesiya were cultivated in a field using the composition containing a fermented eucalyptus product obtained in
Example 2, where the field of implementation site had an altitude of 1,300 m, an average annual temperature of 18.9 °C, and an average annual rainfall of 1,500 mm.
[0089] Specifically, on March 29, 2019, the composition containing a fermented eucalyptus product obtained in Example 2 was mixed with laterite in a volume ratio of 3:7, an obtained mixture was filled with 1,000 bags of 0.6 L non-woven nutrition bags, where each nutrition bag was sown with 1 seed; after 30 days of sowing, the Pinus kesiya seeds had a germination rate of 92.9%, and 5-12 weeds appeared per 100 nutrition bags, with the incidence of weeds of 5% to 12%.
[0090] Use Example 2-2
[0091] Use example 2-2 was conducted according to the method of Use
Example 2-1, except that: in the field, 1,000 bags of 0.6 L non-woven nutrition bags were filled with pure laterite in Use Example 2-1, where each nutrition bag was sown with 1 seed; after 30 days of sowing, the
Pinus kesiya seeds had a germination rate of 89.1%, and 17-30 weeds appeared per 100 nutrition bags, with the incidence of weeds of 17% to 30%.
[0092] By comparing Use Example 2-1 and Use Example 2-2, it can be seen that the composition containing a fermented eucalyptus product obtained in Example 2 of the present disclosure can significantly reduce the occurrence of weeds on the premise of ensuring the germination rate of Pinus kesiya seeds, with the incidence of weeds decreased by 29.41% to 83.33%.
[0093] Use Example 3-1
[0094] Seedbed seedlings of a Chinese herbal medicine, Hemsleya were cultivated in a field using the composition containing a fermented eucalyptus product obtained in Example 3, where the field of implementation site had an altitude of 2,400 m, an average annual temperature of 13.6 °C, and an average annual rainfall of 1,200 mm.
[0095] Specifically, on March 19, 2021, 1 cm to 2 cm of the composition containing a fermented eucalyptus product obtained in Example 3 was laid evenly on a sandy loam-based seedling bed with a flat surface, an area of 10 m? and a thickness of 15 cm to 20 cm, and 400 seedlings (tubers) of Hemsleya were planted per square meter. After 45 days of planting, the incidence of weeds was counted, where 21-33 weeds appeared per 1 m? of the seedling bed, with the incidence of weeds of 5.25% to 8.25%.
[0096] Use Example 3-2
[0097] Use example 3-2 was conducted according to the method of Use
Example 3-1, except that: sandy loam was further laid on the sandy loam- based seedling bed with a flat surface and a thickness of 15 cm to 20 cm, and 400 seedlings (tubers) of Hemsleya were planted per square meter.
After 45 d of planting, the incidence of weeds was counted, where 56-74 weeds appeared per 1 m? of the seedling bed, with the incidence of weeds of 14% to 18.5%.
[0098] By comparing Use Example 3-1 and Use Example 3-2, it can be seen that the composition containing a fermented eucalyptus product obtained in Example 3 of the present disclosure can significantly reduce the occurrence of weeds during the planting of Hemsleya seedlings (tubers), with the incidence of weeds reduced by 40.07% to 71.62%.
[0099] In the present disclosure, the composition containing a fermented eucalyptus product has both a moderate fertilizer efficiency of organic fertilizers and stable physical properties of seedling substrates; the composition could effectively inhibit the germination of weed seeds in the soil while ensuring the normal growth and development of crops and forest tree seedlings, and could reduce the occurrence rate of weeds in the seedling process by 29.41% to 83.45%; meanwhile, only when having a content of Grandinol at 200 mg/kg to 1,000 mg/kg, the composition containing a fermented eucalyptus product could promote the growth of seedlings and inhibit the weeds; Grandinol cannot effectively inhibit the germination of weed seeds at a too low content, and will inhibit the growth and development of crops and forest tree seedlings at a too high content.
[00100] Although the above embodiments have described the present disclosure in a thorough manner, it is only some but not all embodiments of the present disclosure. Other embodiments may be obtained without inventive step according to the present embodiments, all of which fall within the scope of protection the present disclosure.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173891A (en) * | 2010-12-15 | 2011-09-07 | 国家***桉树研究开发中心 | Method for processing light organic substrate on basis of taking eucalyptus bark as raw material |
| CN102531719B (en) * | 2011-12-15 | 2013-12-25 | 江门市华康精细化工有限公司 | Method for preparing organic fertilizer with eucalyptus bark and leaves as raw materials |
| CN106431655B (en) * | 2016-09-26 | 2020-04-17 | 中国林业科学研究院资源昆虫研究所 | Plant-source organic pesticide fertilizer taking eucalyptus material, neem seed and rosemary leaf as raw materials and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173891A (en) * | 2010-12-15 | 2011-09-07 | 国家***桉树研究开发中心 | Method for processing light organic substrate on basis of taking eucalyptus bark as raw material |
| CN102531719B (en) * | 2011-12-15 | 2013-12-25 | 江门市华康精细化工有限公司 | Method for preparing organic fertilizer with eucalyptus bark and leaves as raw materials |
| CN106431655B (en) * | 2016-09-26 | 2020-04-17 | 中国林业科学研究院资源昆虫研究所 | Plant-source organic pesticide fertilizer taking eucalyptus material, neem seed and rosemary leaf as raw materials and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| YONEYAMA KOICHI ET AL: "Effects of grandinol and related phloroglucinol derivatives on transpiration and stomatal closure", PLANT GROWTH REGULATION, vol. 19, no. 1, 1 June 1996 (1996-06-01), Dordrecht, pages 7 - 11, XP055967867, ISSN: 0167-6903, Retrieved from the Internet <URL:http://link.springer.com/article/10.1007/BF00024396/fulltext.html> DOI: 10.1007/BF00024396 * |
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