MXPA06014461A - Electroporador que tiene un miembro hueco alargado. - Google Patents

Electroporador que tiene un miembro hueco alargado.

Info

Publication number
MXPA06014461A
MXPA06014461A MXPA06014461A MXPA06014461A MXPA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A
Authority
MX
Mexico
Prior art keywords
hollow member
elongated hollow
electroporation
electroporator
elongated
Prior art date
Application number
MXPA06014461A
Other languages
English (en)
Inventor
-Keun Jun Chan
Keun-Chang Cho
Chan-Ii Chung
Young-Shik Shin
Jeong-Ah Kim
Neon-Cheol Jung
Original Assignee
Digital Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Digital Biotechnology Co Ltd filed Critical Digital Biotechnology Co Ltd
Publication of MXPA06014461A publication Critical patent/MXPA06014461A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un aparato de electroporacion que contiene miembro hueco alargado para poder proporcionar un campo electrico uniforme durante la electroporacion, donde especificamente, la electroporacion se lleva a cabo al aplicar impulsos electricos a traves de un par de electrodos desde ambas partes extremas del miembro hueco alargado, despues de que el miembro hueco se carga con el especimen de fluido que incluye celulas y material que puede inyectarse en las celulas.
MXPA06014461A 2004-06-12 2005-06-13 Electroporador que tiene un miembro hueco alargado. MXPA06014461A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20040043312 2004-06-12
KR20040088245 2004-11-02
PCT/KR2005/001792 WO2006001614A1 (en) 2004-06-12 2005-06-13 Electroporator having an elongated hollow member

Publications (1)

Publication Number Publication Date
MXPA06014461A true MXPA06014461A (es) 2007-05-23

Family

ID=35781995

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA06014461A MXPA06014461A (es) 2004-06-12 2005-06-13 Electroporador que tiene un miembro hueco alargado.

Country Status (13)

Country Link
US (4) US8932850B2 (es)
EP (1) EP1766004B1 (es)
JP (2) JP4519915B2 (es)
KR (1) KR100853102B1 (es)
CN (1) CN103060191B (es)
AU (1) AU2005257583A1 (es)
BR (1) BRPI0512002A (es)
CA (1) CA2570557C (es)
IL (1) IL179384A0 (es)
MX (1) MXPA06014461A (es)
NO (1) NO20070170L (es)
RU (1) RU2006143560A (es)
WO (1) WO2006001614A1 (es)

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WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
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US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
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Also Published As

Publication number Publication date
US20190040350A1 (en) 2019-02-07
KR100853102B1 (ko) 2008-08-21
JP4519915B2 (ja) 2010-08-04
AU2005257583A1 (en) 2006-01-05
JP2010178746A (ja) 2010-08-19
US20220154128A1 (en) 2022-05-19
US8932850B2 (en) 2015-01-13
US9890355B2 (en) 2018-02-13
EP1766004B1 (en) 2016-08-31
JP2008502356A (ja) 2008-01-31
US20070275454A1 (en) 2007-11-29
IL179384A0 (en) 2007-03-08
NO20070170L (no) 2007-03-12
CA2570557C (en) 2011-01-25
BRPI0512002A (pt) 2008-01-22
WO2006001614A1 (en) 2006-01-05
RU2006143560A (ru) 2008-07-20
CN103060191A (zh) 2013-04-24
US20150093813A1 (en) 2015-04-02
US11130936B2 (en) 2021-09-28
EP1766004A4 (en) 2009-05-06
CA2570557A1 (en) 2006-01-05
EP1766004A1 (en) 2007-03-28
KR20070064556A (ko) 2007-06-21
CN103060191B (zh) 2015-03-11

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