MXPA05006702A - Hemoglobina modificada y metodos para hacer la misma. - Google Patents

Hemoglobina modificada y metodos para hacer la misma.

Info

Publication number
MXPA05006702A
MXPA05006702A MXPA05006702A MXPA05006702A MXPA05006702A MX PA05006702 A MXPA05006702 A MX PA05006702A MX PA05006702 A MXPA05006702 A MX PA05006702A MX PA05006702 A MXPA05006702 A MX PA05006702A MX PA05006702 A MXPA05006702 A MX PA05006702A
Authority
MX
Mexico
Prior art keywords
methods
making same
modified hemoglobin
modified
peg chains
Prior art date
Application number
MXPA05006702A
Other languages
English (en)
Inventor
N Manjula Belur
Original Assignee
Einstein Coll Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Einstein Coll Med filed Critical Einstein Coll Med
Publication of MXPA05006702A publication Critical patent/MXPA05006702A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/105831—Protein or peptide standard or control [e.g., hemoglobin, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion proporciona una molecula de hemoglobina (Hh) que tiene seis ° una cadenas PEG, en donde dos de las cadenas PEG estan ligadas a Cys-93(°) de Hb, y las cadenas PEG restantes estan ligadas a grupos tiol introducidos en ?-NH de Hb. La presente invencion tambien proporciona un proceso para preparar una molecula de hemoglobina modificada (Hb), que comprende los pasos de: (a) hacer reaccionar Hb con un exceso de 8-15 veces de iminotiolano para formar Hb tiolada; y (b) hacer reaccionar la Hb tiolada con exceso de 16-30 veces de PEG funcionalizado con una fraccion de maleimida, para formar la Hb modificada.
MXPA05006702A 2002-12-23 2003-12-18 Hemoglobina modificada y metodos para hacer la misma. MXPA05006702A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43614902P 2002-12-23 2002-12-23
PCT/US2003/040407 WO2004058291A1 (en) 2002-12-23 2003-12-18 Modified hemoglobin and methods of making same

Publications (1)

Publication Number Publication Date
MXPA05006702A true MXPA05006702A (es) 2005-09-30

Family

ID=32682349

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05006702A MXPA05006702A (es) 2002-12-23 2003-12-18 Hemoglobina modificada y metodos para hacer la misma.

Country Status (16)

Country Link
US (1) US7501499B2 (es)
EP (1) EP1585538B1 (es)
JP (1) JP2006514652A (es)
KR (1) KR101086138B1 (es)
CN (1) CN1741812B (es)
AT (1) ATE497776T1 (es)
AU (1) AU2003299700B2 (es)
BR (1) BR0317721A (es)
CA (1) CA2511494A1 (es)
DE (1) DE60336018D1 (es)
ES (1) ES2360215T3 (es)
MX (1) MXPA05006702A (es)
MY (1) MY135256A (es)
SA (1) SA04250032B1 (es)
TW (1) TW200505941A (es)
WO (1) WO2004058291A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1894573A3 (en) * 2002-01-11 2013-06-12 Sangart, Inc. Methods and compositions for oxygen transport comprising a high oxygen affinity
US20030153491A1 (en) * 2002-01-11 2003-08-14 Winslow Robert M. Methods and compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin
US7144989B2 (en) 2002-03-25 2006-12-05 Albert Einstein College Of Medicine Of Yeshiva University Pegylated non-hypertensive hemoglobins, methods of preparing same, and uses thereof
US7521174B2 (en) * 2003-12-05 2009-04-21 Albert Einstein College Of Medicine Of Yeshiva University Universal red blood cells, methods of preparing same, and uses thereof
CA2578232C (en) * 2004-08-31 2015-12-29 Sangart, Inc. Methods to enhance hemodynamic stability using oxygen carrying compositions
US20060134694A1 (en) * 2004-12-22 2006-06-22 Intel Corporation Methods of protein profiling by thiolation
WO2006096774A2 (en) 2005-03-07 2006-09-14 Sangart, Inc. Composition and methods for delivering carbon monoxide (co) and nitric ozide (no) co to tissue using heme proteins as carriers
WO2007050121A2 (en) * 2005-05-11 2007-05-03 Albert Einstein College Of Medicine Of Yeshiva University Site specific pegylated hemoglobin, method of preparing same, and uses thereof
US8741832B2 (en) * 2005-06-10 2014-06-03 Albert Einstein College Of Medicine Of Yeshiva University Pegylated albumin and uses thereof
US8071546B2 (en) 2005-06-10 2011-12-06 La Jolla Bioengineering Institute Uses of pegylated albumin
US7795401B2 (en) * 2006-09-06 2010-09-14 National Chung Cheng University Modified hemoglobin with allosteric effector conjugated
US8697645B2 (en) * 2007-03-13 2014-04-15 Albert Einstein College Of Medicine Of Yeshiva University Second generation low oxygen affinity pegylated hemoglobins as oxygen-carrying plasma expanders
CN101306196B (zh) * 2008-07-03 2011-05-11 中国科学院长春应用化学研究所 一种生物降解的键合了血红蛋白分子的纳米粒子及制法
US10172949B2 (en) 2009-06-09 2019-01-08 Prolong Pharmaceuticals, LLC Hemoglobin compositions
US10172950B2 (en) 2009-06-09 2019-01-08 Prolong Pharmaceuticals, LLC Hemoglobin compositions
DK2440239T3 (en) * 2009-06-09 2017-10-16 Prolong Pharmaceuticals Llc HEMOGLOBIN FORMATIONS
WO2011106396A1 (en) * 2010-02-25 2011-09-01 Sangart, Inc. Methods for preparing peg-hemoglobin conjugates using reduced reactant ratios
US7989593B1 (en) 2010-05-27 2011-08-02 Bing Lou Wong Method for the preparation of a high-temperature stable oxygen-carrier-containing pharmaceutical composition and the use thereof
US7932356B1 (en) 2010-06-23 2011-04-26 Bing Lou Wong Method for the preparation of a heat stable oxygen carrier-containing pharmaceutical composition
US8048856B1 (en) 2010-06-23 2011-11-01 Billion King, Ltd. Treatment methods using a heat stable oxygen carrier-containing pharmaceutical composition
US8084581B1 (en) 2011-04-29 2011-12-27 Bing Lou Wong Method for removing unmodified hemoglobin from cross-linked hemoglobin solutions including polymeric hemoglobin with a high temperature short time heat treatment apparatus
US20130052232A1 (en) 2011-08-31 2013-02-28 Bing Lou Wong Method for the preparation of a heat stable oxygen carrier-containing composition facilating beta-beta cross-linking
MX358785B (es) 2012-03-29 2018-09-04 Sangart Inc Hemoglobina pegilada reticulada con diaspirina.
AU2013243875B2 (en) 2012-04-03 2017-11-30 Schindler, William Succinimide-activated nitroxyl compounds and methods for the use thereof for nitroxylation of proteins
CA2906878C (en) 2013-03-15 2021-09-21 Sangart, Inc. Polyalkylene oxide valerate hemoglobin conjugates

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234903A (en) * 1989-11-22 1993-08-10 Enzon, Inc. Chemically modified hemoglobin as an effective, stable non-immunogenic red blood cell substitute
US5585484A (en) * 1995-04-19 1996-12-17 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Hemoglobin crosslinkers
US5900402A (en) * 1997-05-29 1999-05-04 Enzon, Inc. Method of reducing side effects associated with administration of oxygen-carrying proteins
US6773613B1 (en) * 1998-10-15 2004-08-10 Sangart, Inc. Method for production of stroma-free hemoglobin
AU2002320628A1 (en) * 2001-07-19 2003-03-03 Albert Einstein College Of Medecine Of Yeshiva University Size enhanced hemoglobins: surface decoration and crosslinking of the protein with polyoxy akylene glycols
US20030153491A1 (en) * 2002-01-11 2003-08-14 Winslow Robert M. Methods and compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin
EP1469811A4 (en) * 2002-01-11 2006-01-04 Sangart Inc METHOD AND COMPOSITIONS FOR OXYGEN TRANSPORT WITH MODIFIED HEMOGLOBIN IN PLASMA
US7144989B2 (en) * 2002-03-25 2006-12-05 Albert Einstein College Of Medicine Of Yeshiva University Pegylated non-hypertensive hemoglobins, methods of preparing same, and uses thereof
US7521174B2 (en) * 2003-12-05 2009-04-21 Albert Einstein College Of Medicine Of Yeshiva University Universal red blood cells, methods of preparing same, and uses thereof
WO2007050121A2 (en) 2005-05-11 2007-05-03 Albert Einstein College Of Medicine Of Yeshiva University Site specific pegylated hemoglobin, method of preparing same, and uses thereof

Also Published As

Publication number Publication date
MY135256A (en) 2008-03-31
KR20050084460A (ko) 2005-08-26
ATE497776T1 (de) 2011-02-15
CA2511494A1 (en) 2004-07-15
ES2360215T3 (es) 2011-06-01
US20060135753A1 (en) 2006-06-22
SA04250032B1 (ar) 2007-07-31
AU2003299700B2 (en) 2009-03-26
TW200505941A (en) 2005-02-16
CN1741812B (zh) 2013-04-24
EP1585538B1 (en) 2011-02-09
AU2003299700A1 (en) 2004-07-22
US7501499B2 (en) 2009-03-10
KR101086138B1 (ko) 2011-11-25
BR0317721A (pt) 2005-12-06
CN1741812A (zh) 2006-03-01
WO2004058291A1 (en) 2004-07-15
EP1585538A1 (en) 2005-10-19
EP1585538A4 (en) 2007-11-21
JP2006514652A (ja) 2006-05-11
DE60336018D1 (de) 2011-03-24

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