MXPA04001127A - Ensayo de enlazamiento competitivo para la identificacion de inhibidores de polimerasa vhc. - Google Patents

Ensayo de enlazamiento competitivo para la identificacion de inhibidores de polimerasa vhc.

Info

Publication number
MXPA04001127A
MXPA04001127A MXPA04001127A MXPA04001127A MXPA04001127A MX PA04001127 A MXPA04001127 A MX PA04001127A MX PA04001127 A MXPA04001127 A MX PA04001127A MX PA04001127 A MXPA04001127 A MX PA04001127A MX PA04001127 A MXPA04001127 A MX PA04001127A
Authority
MX
Mexico
Prior art keywords
hcv polymerase
binding assay
competitive binding
identifying inhibitors
identifying
Prior art date
Application number
MXPA04001127A
Other languages
English (en)
Inventor
L Beaulieu Pierre
Original Assignee
Boehringer Ingelheim Ca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Ca Ltd filed Critical Boehringer Ingelheim Ca Ltd
Publication of MXPA04001127A publication Critical patent/MXPA04001127A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Hydrogenated Pyridines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Un metodo para la identificacion de compuestos que se enlazan a la polimerasa VHC que comprende las etapas de: poner en contacto la polimerasa VHC o un analogo de la misma con una Formula de prueba I: (ver formula) en donde A es O, S, N, NR1, o CR1, en donde R1 es como se define en la presente; ---representa ya sea un enlace doble o sencillo; R2 se selecciona de: H, halogeno, R21, OR21, SR21, COOR21, S02N (R22)2, N( R22)2, CON (R22)2, NR22C (O) R22 o NR22C(O)NR22 en donde R21 y cada una de R22 esta definida en la presente; B es NR3 o CR3 en donde R3 es como se define en la presente; con la condicion de que cuando A no es N, luego una de A o B es ya sea CR1 o CR3, K es N o CR4, en donde R4 esta definida en la presente; L es N o CR5, en donde R5 tiene la misma definicion como R4 como se ha definido anteriormente. M es N o CR7, en donde R7 tiene la misma definicion como R4 como se ha definido anteriormente; R5 es C(Y1)Z en donde Y1 es O o S; y Z es N(R6a)R6 o OR6, en donde R6a es H o alquilo o NR61R62 en donde R61 y R62 estan definidas en la presente; y R6 es H, alquilo, cicloalquilo, alquenilo, Het, alquil-arilo, alquil-Het o R6 es En donde R7, R8 y Q son como se han definido en la presente; Y2 es O o S; R9 es H, alquilo de (C1-6), cicloalquilo de (C3-7) o alquilo de (C1-6)-cicloalquilo de (C3-7), arilo, Het, alquilo de (C1-6)-arilo o alquilo de (C1-6)-Het los cuales estan opcionalmente sustituidos con R90; o R9 se enlaza covalentemente a ya sea R7 o R8 para formar un heterociclo de 5- o 6-miembros; o una sal de la misma; en donde la prueba comprende una etiqueta detectable que se une cualquier posicion adecuada, con lo cual la prueba se enlaza a una polimerasa VHC o a un analogo de la misma y que es capaz de ser desplazada por un inhibidor de la misma.
MXPA04001127A 2001-08-07 2002-08-05 Ensayo de enlazamiento competitivo para la identificacion de inhibidores de polimerasa vhc. MXPA04001127A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31027201P 2001-08-07 2001-08-07
PCT/CA2002/001214 WO2003014377A2 (en) 2001-08-07 2002-08-05 Competitive binding assay for identifying inhibitors of hcv polymerase

Publications (1)

Publication Number Publication Date
MXPA04001127A true MXPA04001127A (es) 2004-07-08

Family

ID=23201740

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA04001127A MXPA04001127A (es) 2001-08-07 2002-08-05 Ensayo de enlazamiento competitivo para la identificacion de inhibidores de polimerasa vhc.

Country Status (13)

Country Link
US (1) US7294457B2 (es)
EP (1) EP1417493B1 (es)
JP (1) JP4646514B2 (es)
AT (1) ATE475095T1 (es)
CA (1) CA2450142C (es)
DE (1) DE60237089D1 (es)
DK (1) DK1417493T3 (es)
ES (1) ES2347954T3 (es)
HU (1) HUP0400670A3 (es)
IL (1) IL159981A0 (es)
MX (1) MXPA04001127A (es)
NZ (2) NZ531488A (es)
WO (1) WO2003014377A2 (es)

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US9029196B2 (en) 2003-11-10 2015-05-12 Stats Chippac, Ltd. Semiconductor device and method of self-confinement of conductive bump material during reflow without solder mask
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TW202334164A (zh) 2022-01-12 2023-09-01 美商戴納立製藥公司 （Ｓ）－５－苄基－Ｎ－（５－甲基－４－側氧基－２，３，４，５－四氫吡啶並［３，２－ｂ］［１，４］氧氮呯－３－基）－４Ｈ－１，２，４－***－３－甲醯胺的晶型

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Also Published As

Publication number Publication date
JP4646514B2 (ja) 2011-03-09
US7294457B2 (en) 2007-11-13
DE60237089D1 (de) 2010-09-02
CA2450142C (en) 2009-12-22
NZ531488A (en) 2008-06-30
JP2005500055A (ja) 2005-01-06
EP1417493A2 (en) 2004-05-12
US20030108862A1 (en) 2003-06-12
ES2347954T3 (es) 2010-11-26
WO2003014377A2 (en) 2003-02-20
CA2450142A1 (en) 2003-02-20
HUP0400670A3 (en) 2006-06-28
HUP0400670A2 (en) 2006-03-28
EP1417493B1 (en) 2010-07-21
WO2003014377A3 (en) 2003-12-18
DK1417493T3 (da) 2010-10-04
ATE475095T1 (de) 2010-08-15
NZ554933A (en) 2009-01-31
IL159981A0 (en) 2004-06-20

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