MXPA03004162A - Metodos para produccion a gran escala de proteinas en procariotes. - Google Patents
Metodos para produccion a gran escala de proteinas en procariotes.Info
- Publication number
- MXPA03004162A MXPA03004162A MXPA03004162A MXPA03004162A MXPA03004162A MX PA03004162 A MXPA03004162 A MX PA03004162A MX PA03004162 A MXPA03004162 A MX PA03004162A MX PA03004162 A MXPA03004162 A MX PA03004162A MX PA03004162 A MXPA03004162 A MX PA03004162A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- prokaryotes
- protein production
- large scale
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
El invento pertenece al sector de la produccion de proteinas en celulas procarioticas. El invento se refiere a metodos para la produccion de una proteina heterologa que se deriva de un ADN recombinante en celulas procarioticas, en el que dicha proteina heterologa se segrega extracelularmente como una proteina activa y correctamente plegada, y la celula procariotica contiene y expresa un vector que comprende el ADN que codifica dicha proteina heterologa, unido operativamente con el ADN que codifica el peptido de senal OmpA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0027782.2A GB0027782D0 (en) | 2000-11-14 | 2000-11-14 | Methods fro large scale protein productio in prokaryotes |
PCT/EP2001/012920 WO2002040696A2 (en) | 2000-11-14 | 2001-11-08 | Methods for large scale protein production in prokaryotes |
Publications (1)
Publication Number | Publication Date |
---|---|
MXPA03004162A true MXPA03004162A (es) | 2004-04-20 |
Family
ID=9903155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MXPA03004162A MXPA03004162A (es) | 2000-11-14 | 2001-11-08 | Metodos para produccion a gran escala de proteinas en procariotes. |
Country Status (18)
Country | Link |
---|---|
EP (1) | EP1343909B1 (es) |
JP (1) | JP2004513648A (es) |
KR (1) | KR100857402B1 (es) |
CN (1) | CN100457909C (es) |
AR (1) | AR031336A1 (es) |
AT (1) | ATE440145T1 (es) |
AU (1) | AU2002221824A1 (es) |
CA (1) | CA2428643A1 (es) |
CY (1) | CY1110546T1 (es) |
DE (1) | DE60139635D1 (es) |
DK (1) | DK1343909T3 (es) |
ES (1) | ES2332124T3 (es) |
GB (1) | GB0027782D0 (es) |
HK (1) | HK1077848A1 (es) |
MX (1) | MXPA03004162A (es) |
PT (1) | PT1343909E (es) |
UY (1) | UY27021A1 (es) |
WO (1) | WO2002040696A2 (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7087412B2 (en) | 2000-11-14 | 2006-08-08 | Boehringer Ingelheim International Gmbh | Methods for large scale protein production in prokaryotes |
US6955910B2 (en) | 2000-11-14 | 2005-10-18 | Boehringer Ingelheim International Gmbh | Method for large scale production of recombinant DNA-derived TPA or K2S molecules |
GB0027779D0 (en) * | 2000-11-14 | 2000-12-27 | Boehringer Ingelheim Int | Methods for large scale production of recombinant dna-derived tpa or k2s molecules |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
PL2336153T3 (pl) | 2003-11-21 | 2016-08-31 | Pfenex Inc | Udoskonalone systemy ekspresji z układem wydzielania SEC |
EP2412816B1 (en) | 2004-07-26 | 2014-12-03 | Pfenex Inc. | Process for improved protein expression by strain engineering |
CA2677179C (en) | 2007-01-31 | 2016-02-16 | Dow Global Technologies Inc. | Bacterial leader sequences for increased expression |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
EP2142651B1 (en) | 2007-04-27 | 2013-05-22 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
MY159680A (en) * | 2009-11-17 | 2017-01-13 | Janssen Biotech Inc | Improved bacterial membrane protein secrection |
CN102924602B (zh) * | 2011-08-08 | 2015-06-17 | 深圳市艾派格生物技术有限公司 | 金离子吸附蛋白表面展示***及富集并回收金离子的方法 |
CN106188311B (zh) * | 2016-07-18 | 2020-08-04 | 山东大学齐鲁医院 | 一种重组肿瘤坏死因子相关细胞凋亡诱导配体蛋白的制备方法与应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
EP0467676A3 (en) * | 1990-07-18 | 1992-11-19 | Applied Immunesciences, Inc. | Temperature-inducible secretion expression vectors and their use in prokaryotic cells |
WO1997038123A1 (en) * | 1996-04-05 | 1997-10-16 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
EP1048732A1 (de) * | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
-
2000
- 2000-11-14 GB GBGB0027782.2A patent/GB0027782D0/en not_active Ceased
-
2001
- 2001-11-08 EP EP01996619A patent/EP1343909B1/en not_active Expired - Lifetime
- 2001-11-08 ES ES01996619T patent/ES2332124T3/es not_active Expired - Lifetime
- 2001-11-08 AT AT01996619T patent/ATE440145T1/de active
- 2001-11-08 KR KR1020037006564A patent/KR100857402B1/ko not_active IP Right Cessation
- 2001-11-08 MX MXPA03004162A patent/MXPA03004162A/es active IP Right Grant
- 2001-11-08 JP JP2002543008A patent/JP2004513648A/ja not_active Withdrawn
- 2001-11-08 AU AU2002221824A patent/AU2002221824A1/en not_active Abandoned
- 2001-11-08 WO PCT/EP2001/012920 patent/WO2002040696A2/en active Application Filing
- 2001-11-08 DK DK01996619T patent/DK1343909T3/da active
- 2001-11-08 CA CA002428643A patent/CA2428643A1/en not_active Abandoned
- 2001-11-08 PT PT01996619T patent/PT1343909E/pt unknown
- 2001-11-08 CN CNB018188974A patent/CN100457909C/zh not_active Expired - Fee Related
- 2001-11-08 DE DE60139635T patent/DE60139635D1/de not_active Expired - Lifetime
- 2001-11-12 UY UY27021A patent/UY27021A1/es not_active Application Discontinuation
- 2001-11-14 AR ARP010105300A patent/AR031336A1/es unknown
-
2005
- 2005-11-04 HK HK05109852.3A patent/HK1077848A1/xx not_active IP Right Cessation
-
2009
- 2009-11-12 CY CY20091101181T patent/CY1110546T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
ATE440145T1 (de) | 2009-09-15 |
ES2332124T3 (es) | 2010-01-27 |
JP2004513648A (ja) | 2004-05-13 |
CY1110546T1 (el) | 2015-04-29 |
AR031336A1 (es) | 2003-09-17 |
HK1077848A1 (en) | 2006-02-24 |
EP1343909A2 (en) | 2003-09-17 |
WO2002040696A3 (en) | 2002-09-19 |
CN100457909C (zh) | 2009-02-04 |
CN1628176A (zh) | 2005-06-15 |
WO2002040696A2 (en) | 2002-05-23 |
KR100857402B1 (ko) | 2008-09-08 |
PT1343909E (pt) | 2009-09-11 |
EP1343909B1 (en) | 2009-08-19 |
DK1343909T3 (da) | 2009-12-21 |
GB0027782D0 (en) | 2000-12-27 |
AU2002221824A1 (en) | 2002-05-27 |
UY27021A1 (es) | 2002-07-31 |
CA2428643A1 (en) | 2002-05-23 |
KR20030051821A (ko) | 2003-06-25 |
DE60139635D1 (de) | 2009-10-01 |
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Legal Events
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FG | Grant or registration |