MXPA01012844A

MXPA01012844A - Corynebacterium glutamicum.

Info

Publication number: MXPA01012844A
Application number: MXPA01012844A
Authority: MX
Inventors: Markus Pompejus
Original assignee: Basf Ag
Priority date: 1999-06-25
Filing date: 2000-06-23
Publication date: 2002-07-09
Also published as: HUP0203340A2; TR200103709T2; KR20030000013A; KR20070087094A; KR100878335B1; KR100834986B1; JP2007244392A; ES2184658T1; CA2380870A1; KR20070087095A; AU5836900A; KR20060118631A; PL359863A1; JP2003525593A; AU783703B2; WO2001000804A2; EP1290178A2; WO2001000804A3; SK18882001A3; JP2007244391A

Abstract

Isolated nucleic acid molecules, designated SRT nucleic acid molecules, which encode novel SRT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SRT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SRT proteins, mutated SRT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SRT genes in this organism.

Description

CORYNEBACTERIUM GLUTAMICUM GENES CODING TOLERANCE AND STRESS RESISTANCE PROTEINS Related Requests This application claims priority of the previously filed US Provisional Patent application Serial No. 60/141031, filed on June 25, 1999, US Provisional Patent Application Serial No. 60/142692, filed July 1, 1999, and also United States Provisional Patent Application Serial No. 60/151214, filed on August 27, 1999. This application also claims priority of the Application for German Patent No. 19930429.7, filed on July 1, 1999, German Patent Application No. 19931413.6, filed July 8, 1999, German Patent Application No. 19931457.8, filed July 8, 1999, Application of German Patent No. 19931541.8, filed July 8, 1999, German Patent Application No. 19932209.0, filed on July 9, 19 99, German Patent Application No. 19932230.9, filed on July 9, 1999, German Patent Application No. 19932914.1, filed on July 14, 1999, German Patent Application No. 19940764.9, filed on August 27, 1999. 1999, and German Patent Application No. 19941382.7, filed on August 31, 1999. The complete contents and all the applications mentioned above are hereby expressly incorporated in their entirety by reference. BACKGROUND OF THE INVENTION Certain products and byproducts of metabolic processes that occur naturally in cells are useful in a wide range of industries, including the food, cosmetic and pharmaceutical industries. These molecules, collectively known as "fine chemicals" include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatics, vitamins and cofactors and enzymes. Its production is most conveniently carried out through large scale cultivation of bacteria developed to produce and secrete large quantities of a particularly desired molecule. A particularly useful organism for this purpose is Ccrynebacterx um gl uta xcum, a non-pathogenic, gram-positive bacterium. Through selection by staining, numerous strains have been developed which produce a range of desirable compounds. However, the selection of improved strains for the production of a particular molecule is a difficult process that requires a lot of time. COMPENDIUM OF THE INVENTION The invention offers novel molecules of bacterial nucleic acid that tieper. Various uses. These uses include the detection of microorganisms that can be used to produce fine chemicals, the modulation of the production of fine chemicals in C. gl utamxcum or related bacteria, the determination of the type or identification of C. gl utamxcum or related bacteria, as points of reference for the genome mapping of C. gl utamx cum, and as markers for transformation. These novel nucleic acid molecules encode proteins, which are known herein as tolerance and stress resistance (SRT) proteins. C. gl utamxcum is an aerobic, gram-positive bacterium commonly used in the industry for the large-scale production of several fine chemicals and also for the degradation of hydrocarbons (as, for example, in the case of oil spills) and for oxidation of terpenoids. The SRT nucleic acid molecules of the invention can therefore be used to identify microorganisms that can be used to produce fine chemicals such as, for example, by fermentation processes. The modulation of the expression of the SRT nucleic acids of the invention, or modification of the sequence of the SRT nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism (for example, to improve the yield or production of one or more chemicals from a species, Corynebacterium or Brevibacterium).

The SRT nucleic acids of the invention can also be used to identify an organism as being Corynebacterxum gl utamxcum or a closely related organism thereof, or to identify the presence of C. gl utamxcum or a related organism thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of numerous genes of C. gl utamicum, - by probing the genomic DNA extracted from a culture of a single or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. gl uta zxcum which is unique to this organism, it can be determined if this organism is present. Although Corynebacterx um gl utamx itself is not pathogenic, it is related to pathogenic species for humans such as Corynebacterxum diphtheri ae (the causative agent of diphtheria); The detection of these organisms is of significant clinical relevance. The SRT nucleic acid molecules of the invention can also serve as reference points for mapping the genome of C. gl utami cum or genomes of related organisms. Similarly, these molecules or variants or portions thereof may serve as markers for genetically engineered Corynebacterx um or Brevibacterx um species. The SRl proteins encoded by the novel nucleic acid molecules of the invention can, for example, allow the survival of C. gl utami cum in an environment either chemically or environmentally hazardous to this microorganism. Given the availability of cloning vectors for use in Corynebacterx um gl utamxcum, such as those disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C. glutamxcum and related Brevibacterium species (e.g. lactofermentum) (Yoshihama et al., J. Bacteriol., 162: 591-597 (1985); Katsumata et al., J. Bacteriol., 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the present invention can be used in the genetic manipulation of this organism to make said organism a better or more efficient producer of one or more fine chemicals, through of the capacity of these proteins to allow the growth and multiplication of C. gl utamxcum (and also the continuous production of one or several fine chemicals) in circumstances that would normally prevent the growth of said organism, such as the conditions frequently encountered during large-scale fermentative cultivation. For example, by overexpression or manipulation of a protease molecule induced by heat shock such that it has an optimized activity, the ability of the bacterium to degrade incorrectly bent proteins when the bacterium is challenged at high temperatures can be increased. . Having a lower number of misfolded proteins (and possibly erroneously regulated or non-functional) to interfere with normal reaction mechanisms in the cell, increases the ability of the cell to function normally in a culture of this type, which it must in turn provide increased viability. This overall increase in the number of cells that have greater viability and activity in the crop should also result in an increase in yield, production, and / or production efficiency of one or more desired fine chemicals, due to at least the relatively greater number of cells that produce these chemicals in the crop. This invention offers novel SRT nucleic acid molecules that encode SRT proteins that can allow the survival of C. gl utamxcum in an environment either chemically or environmentally hazardous to this microorganism. Nucleic acid molecules encoding a SRT protein are referred to herein as SRT nucleic acid molecules. In a preferred embodiment, the SRT protein participates in metabolic pathways that allow the survival of C. gl utami cum in an environment either chemically or environmentally dangerous for this microorganism. Examples of proteins of this type include the proteins encoded by the genes presented in Table 1. Accordingly, one aspect of the invention relates to isolated nucleic acid molecules (such as, for example, cDNA, DNA, or RNA) comprising a nucleotide sequence encoding a SRT protein or biologically active portion thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of nucleic acid encoding SRT (such as for example AD? or AR? m). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences presented in SEQ ID Nos with odd numbers in the Sequence List (eg, SEQ ID? 0: 1, SEQ ID? O: 3 , SEQ ID? 0: 5, SEQ ID? 0: 7 ...) either the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments the isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes or is at least 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90% and even more preferably at least about 95%, 96%, 97%, 98%, 99% homologous with a presented nucleotide sequence, such as a SEQ ID? O: of odd number in the List of Sequences (such as, ?? Q ID MO: l, SEQ I MO: 3, SEQ ID? O: 5, SEQ ID? O: 7 ...) or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences presented in a SEQ ID NO: numbered evenly in the Sequence List (such as, for example, SEQ ID NO: 2, SEQ ID NO: 4 , SEQ ID NO: 6, SEQ ID NO: 8 ...) The preferred SRT proteins of the present invention also possess at least one of the SRT activities described above. In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence that is sufficiently homologous with an amino acid sequence of the invention (such as, for example, a sequence having a SEQ ID NO: of even number in the Sequence List), as exemplified by a sufficient homology with an amino acid sequence of the invention such that the protein or portion thereof retains SRT activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to increase the survival of C. gl utamxcum in an environment that is chemically or environmentally hazardous to this microorganism. In one embodiment, the protein encoded by the nucleic acid molecule has a homology of at least about 50%, preferably at least about 61%, and more preferably at least about 70%, 0%, or 90% and most especially at least about 95%, 96%, 97%, 98% or 99% or more with an amino acid sequence of the invention (eg, an entire amino acid sequence selected from the that have a SEQ ID: of even number in the Sequence List). In another preferred embodiment, the protein is a full-length C. gl utamx cum protein substantially homologous with a whole amino acid sequence of the invention (encoded by the open reading frame shown in the corresponding odd number SEQ ID NO: samples). in the Sequence List (for example, SEQ ID N0: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7 ...). In another preferred embodiment, the isolated nucleic acid molecule is derived from C. gl utamxcum and encodes a protein (such as, for example, a SRT fusion protein) that includes a biologically active domain having a homology level of at least about 50% or more with a biologically active domain of the amino acid sequences of the invention (such as for example, a sequence of one of the SEQ ID Nos: even number in the Sequence List) and has the ability to increase the survival of C. gl utamx cum in an environment either chemically or environmentally hazardous to this microorganism, or possesses one or more of the activities presented in Table 1, and which also includes heterologous nucleic acid sequences encoding a polypeptide or regulatory regions.

In another embodiment, the isolated nucleic acid molecule is at least 15 nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention (eg, a sequence of SEQ ID NO. : with odd number in the Sequence List). Preferably, the isolated nucleic acid molecule corresponds to a naturally occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C. glutamicum SRT protein, or a biologically active portion thereof. Another aspect of the invention relates to vectors such as, for example, expression vectors, recombinants containing the nucleic acid molecules of the invention, and host cells in which such vectors have been introduced. In one embodiment, said host cell is used to produce a SRT protein by culturing the host cell in a suitable medium. The SRT protein can be isolated from the host cell or medium. In another aspect, the invention relates to a genetically modified microorganism wherein a SRT gene has been introduced or altered. In the embodiment, the genome of the microorganism has been altered by the introduction of a nucleic acid molecule of the invention that constructs a wild-type or mutated SR7 sequence as a transgene. In another embodiment, an endogenous SRT gene within the genome of the microorganism has been altered, for example, functionally affected, by homologous recombination with an altered SRT gene. In another embodiment, an endogenous or endogenous SRT gene introduced into a microorganism has been altered by one or several point mutations, deletions or inversions, but continues to encode a functional SRT protein. In another embodiment, one or more of the regulatory regions (e.g., a promoter, repressor or inducer) of the SRT gene in a microorganism has been altered (e.g., by removal, truncation, inversion, or dot mutation) in such a manner. that the expression of the SRT gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterxum or Brevxbacterxum, with Corynebacterium um glummicum being particularly preferred. In a preferred embodiment, the microorganism is also used for the production of a desired compound, such as for example amino acid, lysine being particularly preferred. In another aspect, the invention provides a method for identifying the presence or activity of Corynebacterxum diphtheria in a subject. This method includes the detection of one or more of the nucleic acid or amino acid sequences of the invention (for example, the sequences presented in the Sequence List as SEQ ID NOs 1 to 304)) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheria in the subject. Another aspect of the present invention relates to an isolated SRT protein or to a portion, for example, a biologically active portion thereof. In a preferred embodiment, the isolated SRT protein or portion thereof has the ability to increase the survival of C. gl utamxcum in a chemical or environmentally hazardous environment for this microorganism. In a preferred embodiment, the isolated SRT protein or portion thereof is sufficiently homologous with an amino acid sequence of the invention (eg, a sequence of a SEQ ID NO: of even number in the Sequence List) such that the protein or portion of it maintains the ability to increase the survival of C. gl utamxcum in a chemical or environmentally hazardous environment for this microorganism.

The invention also offers an isolated preparation of a SRT protein. In preferred embodiments, the protein of SRT comprises an amino acid sequence of the invention (for example, a sequence of a SEQ ID No: even number of the Sequence List). In another preferred embodiment, the invention relates to a full-length protein isolated substantially homologous to a whole amino acid sequence of the present invention (eg, a SEQ ID NO: sequence of even number from the Sequence List) (encoded for the open reading term established in SEQ ID NO: corresponding odd number in the Sequence List). In another embodiment, the protein has a homology of at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and most especially by at least about 95%, 96%, 97%, 98%, or 99% or more with a whole amino acid sequence of the invention (eg, a sequence of a SEQ ID NO: even number from the Sequence List) ). In other embodiments, the isolated SRT protein comprises an amino acid sequence having a homology of at least about 50% or more with one of the amino acid sequences of the invention (eg, a sequence of a SEQ ID NO: of an even number from the Sequence List) and may improve the survival rate of C. glutamxcum in a chemical or environmentally endangered environment for - this microorganism, or has one or more of the activities presented in Table 1. Alternatively, the protein Isolated SRT may comprise an amino acid sequence that is encoded by the sequence of nucleotides that hybridize, e.g., hybridizes under stringent conditions, or has a homology level of at least about 50%, preferably io less approximately the JIJ, with greater preference at least approximately 70%,? , z 90% and most especially at least about 95%, 96%, 97%, 98% or 99% or more with a nucleotide sequence of one of the SEQ ID NOs with even numbers presented in the Sequence List. It is also preferred that the preferred forms of SRT proteins have one or more of the SRT bioactivities described herein. The SRT polypeptide, or a biologically active portion thereof, can be operatively linked to a non-SRT polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity that differs from the activity of the SRT protein alone. In other preferred embodiments, this fusion protein results in increased yields, increased production and / or higher production efficiency of a desired fine chemical from C. gl utami cum. In another aspect, the invention includes methods for screening molecules that modulate the activity of a SRT protein, either by interaction with the protein itself or a substrate or binding partner of the SRT protein, or by modulation of the protein. transcription or translation of an SRT nucleic acid molecule of the invention. Another aspect of the invention relates to a method for the production of a fine chemical. This method includes the cultivation of a cell containing a vector that directs the expression of an SRT nucleic acid molecule of the invention, such that it produces a fine chemical. In a preferred embodiment, this method includes the step of obtaining a cell containing said vector, wherein a cell is affected with a vector that directs the expression of an SRT nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. in a particularly preferred embodiment, the cell is of the genus Corynebacterxum or Brevxbacterxum or is selected from the strains shown in Table 3. Another aspect of the invention relates to methods for modulating the production of a molecule from a microorganism . Such methods include contacting the cell with an agent that modulates SRT protein activity or SRT nucleic acid expression such that an activity associated with a cell is altered relative to said activity in the absence of the agent. In a preferred embodiment, the cell is modulated in terms of resistance to one or more toxic chemicals or resistance to one or several environmental stresses, so that the yields or rate of production of a fine chemical desired by this microorganism can be increased. The agent that modulates SRT protein activity can be an agent that stimulates SRT protein activity or an expression of SRT nucleic acid. Examples of agents that stimulate SRT protein activity or SRT nucleic acid expression include small molecules, active SRT proteins, and nucleic acids encoding SRT proteins that have been introduced into the cell. Examples of agents that inhibit SRT activity or SRT expression include small molecules, as well as antisense SRT nucleic acid molecules. Another aspect of the invention relates to methods for modulating yields of a desired compound from a cell, which includes the introduction of a mutant or wild type SRT gene into a cell, either maintained in a separate plasmid and one hundred integrated in the genome of the host cell. If it is integrated in the genome, said integration can be random or it can be carried out by homologous recombination in such a way that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical agent is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine. DETAILED DESCRIPTION OF THE INVENTION The present invention provides SR7 protein and nucleic acid molecules involved in the survival of C. gl utamicum when said microorganism is exposed to chemical or environmental hazards. The molecules of the invention can be used in modulating the production of fine chemicals from microorganisms, since these SRT proteins provide a means for the continuous growth and multiplication of C. gl utamxcum in the presence of dangerous environmental conditions or toxic chemicals, conditions that can be found, for example, during large-scale fermentative growth. By increasing the rate of growth or maintaining at least normal growth in unsatisfactory and even toxic conditions, the yield, production, and / or production efficiency of one or more fine chemicals can be increased from a culture of this type, for example due to the relatively larger number of cells that produce the fine chemical in the culture. Aspects of the invention are further explained below. I. FINE CHEMICALS The term "fine chemical" is an expression well known in the art that includes molecules produced by an organism that has applications in various industries such as, for example, without limitation, the pharmaceutical, agricultural and cosmetic industries. Such compounds include organic acids such as tartaric acid, itaconic acid and dia inopiolic acid, both nanoproteinogenic protein crenotene amino acids, purine and pyrimidine bases, nucleosides and nucleotides (in accordance with the writing, for example in Kuninaka, A. ( 1996) Nucleotides and related compounds, pp. 561-612, in Biotechnology vol.6, Rehm et al., Eds VCH: Weinheim, and references contained there), lipids, both saturated and unsaturated fatty acids (eg, arachidonic acid ), diols (for example, propandiol, and butanediol), carbohydrates (for example, hyaluronic acid and trehalose), aromatics (for example, aromatic amines, vanillin, and indigo), vitamins, and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, Vol. A27, "Vitamins", pp. 443-613 (1996) VCH: Weinheim and references there, and Ong, AS, Niki, E. &Packer, L. (1995) "Nutrition, Lipids, Health, and Disease "Abstracts of UNESCO / Confederation of Scientific and Technological Associations in Malaysia, at the Society for Free Radical Research-Asia, which was held from September 1 to 3, 1994 in Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides ( Cañe et al. (1998) Science 282: 63-68), and all other chemical agents described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references mentioned therein. The metabolism and uses of some of these fine chemicals are further explained below. A. Metabolism and use of amino acids Amino acids comprise the basic structural units of all proteins and as such are essential for normal cellular functioning in all organisms. The term "Amino Acids" is known in the art. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, where they are linked by peptide bonds, while the non-proteinogenic amino acids (hundreds of them are known) are not normally found in proteins (see Ulmann 's Encyclopedia of Industrial Chemistry, volume A2, page 57-97 VCH: Weinheim (1985)). Amino acids can be found in the optical configuration D or L, even though amino acids in L configuration are generally the only type found in naturally occurring proteins. The biosynthetic and degradation pathways of each of the 20 proteinogenic amino acids have been well characterized in both prokaryotic and eukaryotic cells (See, for example, Stryer, L. Biochemistry, 3rd edition, pages 578-590 (1988)). The "essential" amino acids (histidine, isoieucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine) so named since they are generally a nutritional requirement due to the complexity of their biosynthesis, are easily converted by simple biosynthetic pathways into the remaining 11"non-essential" amino acids (alanine, argmin, asparagine, aspartate, cysteine, glutamate , glutamine, glycine, proline, serine and tyrosine). Higher animals retain the ability to synthesize some of these amino acids, but essential amino acids must be supplied from the diet for normal protein synthesis to occur. Apart from their function in the biosynthesis of proteins, these amino acids are interesting chemical agents per se, and it has been found that many of them have several applications in the food industries for humans and animals, chemical industry, food industry cosmetics, agriculture as well as pharmaceutical industry. Lysine is an important amino acid in nutrition not only in humans but also in monogastric animals such as birds and pigs. Glutamate is most often used as a flavoring additive rr.opc-sodium glutamate, MSG) and is widely used in the food industry, as well as aspartate phenylalanine, glycine and cysteine. The glycine L-methionine and tryptophan are all used in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, prelina, serine and alanine are used in both the pharmaceutical industry and the cosmetics industry. Threonine, tryptophan and methionine D / L are final additives for food. (Leuchtenberger, W. (1996) Amino Acids Technical Production and Use, page 466-502 in Rehm et al. (Eds.) Biotechnology vol.6, chapter 14a.VCH: Weinheim). In addition, these amino acids are useful as precursors for the synthesis of synthetic amino acids and proteins, such as N-acetylcysteine, S-carboxymethyl-L-cysteine, (S) -5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry , vol. A2, pages 57-97, VCH: Weinheim, 1985. The biosynthesis of these natural amino acids in organisms capable of producing them such as bacteria has been well characterized (for a review of bacterial amino acid biosynthesis and regulation ie the same, see Umbarger , HE (1978) Ann. Rev. Bxoche-. 47: 533-606). Glutamate is synthesized by the reductive amination of a-ketoglutarate, an intermediate product in the citric acid cycle. Glutamine, proline and arginine sen subsequently produced, each one, from glutamate. Serine biosynthesis is a three-step process that starts with 3-phosphoglycerate (an intermediate product in glycolysis), and results in this amino acid after oxidation, transamination, and hydrolysis. Both cysteine and glycine are produced from serine; the first condensation of homocysteine with serine, and the second by the transfer of the β-side chain carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhidroximetiiasa.

Phenylalanine and tyrosine are synthesized from the precursors of the glycolytic pathway and pentose phosphate, erythro-4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differs only in the 2 final steps after the synthesis of prephenate. Tryptophan is also produced from these 2 initial molecules, but its synthesis is a way of 11 steps. Tyrosine can be synthesized from phenylalanine in the reaction catalyzed by phenylalanine hydroxylase. Alanine, valine and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed of oxaloacetate, an intermediate product of the citric acid cycle. Asparagine, methionine, threonine and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex line of 9 steps results in the production of histidine from 5-phosphoribosyl-1-pyrophosphate, an activated sugar. Amino acids in excess of the protein synthesis needs of the cell can not be stored and are degraded to the contrary to provide intermediates for major metabolic pathways of cells (see Stryer, L. Biochemistry 3rd edition, Chapter 21"Amino Acid Degradation and the Urea Cycie "pages 495-516 (1988)). Even though the cell can convert unwanted amino acids into useful metabolic intermediates, the production of amino acids is expensive in terms of energy, precursor molecules and the enzymes needed to synthesize them. It is therefore not surprising that amino acid biosynthesis is regulated by the inhibition of feedback where the presence of a particular amino acid serves to slow down or completely suspend its own production (for a review of the feedback mechanisms in the biosynthetic pathways of amino acids, see Stryer, L. Biochemistry, 3rd edition, chapter 24: "Biosynthesis of Amino Acids and Heme" pages 575-600 (1988)). Thus, the production of any particular amino acid is limited by the amount of this amino acid present in the cell. B. Vitamin metabolism as cofactors and nutraceuticals, and uses Vitamins, cofactors and nutraceuticals comprise another group of molecules that higher animals have lost the ability to synthesize and therefore must ingest, even when they are easily synthesized by other organisms such as bacteria . These molecules are either bioactive substances themselves or precursors of biologically active substances that can serve as electron carriers or intermediates in various metabolic pathways. Apart from their nutritional value, these compounds also have a significant industrial value as coloring agents, antioxidants and catalysts or other processing aids. (For a review of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol.A27, pages 443-613, VCH: Weinheim, 1996.). The term "vitamin" is known in the art and includes nutrients that are required by an organism for normal functioning, but which said organism can not synthesize alone. The group of vitamins can include cofactors and nutraceuticals. The term "cofactors" includes non-proteinaceous compounds required for normal enzymatic activity to occur. Such compounds may be organic and inorganic; The cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements that have health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids (for example, polyunsaturated fatty acids). The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been widely characterized (Ullman's Encyclopaedia of Industrial Chemistry, "Vitamins" vol.A27, pages 443-613, VCH: Weinheim, 1996; Michal. G. (1999) Bicchemical Pathways: An Atlas of Biochemistry and Molecular? Ioiogy, John Wiiey ¿Sons; Ong, A.S., Niki, E. & Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Abstracts of the UNESCO / Confederation of Scientific and Technological Assistances in Malaysia, and the Society for Free Radical Research - Asia, which had verification from September 1 to 3 1994 in Penang, Malaysia, AOCS Press: Champaing, IL X, 374 S). Thiamine (vitamin Bi) is produced by chemical coupling of pyrinidine and thiazole portions. Riboflavin (vitamin B2) is synthesized from guanosine-5 '-triphosphate (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is used for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds known collectively as "vitamin BJ '(eg pyridoxine, pyridoxamine, pyridoxa-5'-phosphate, and pyridoxine hydrochloride used commercially) are all derived from the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R) - (+) - N- (2,4-dihydroxy-3, 3-dimethyl-l-oxobutyl) -β-alanine) can be produced by chemical synthesis or by fermentation. The enzymes responsible for the steps of biosynthesis for the conversion into pantoic acid in β-alanine and for the condensation of pantothenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which biosynthesis is carried out in 5 enzymatic steps: pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of of pantothenate but also the production of pantoic acid- (R), (R) -pantolactone, (R) -pantenol (provitamin B5), pantethein (and its derivatives) and coenzyme A. The biosynthesis of biotin from the The precursor molecule of pimeloil-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. It has also been found that many of the corresponding proteins were involved in the synthesis of Fe groups and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. Folates are a group of substances that are derived from folic acid which in turn is derived from L-glutamic acid, p-amino-benzoic acid and 6-methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5 'triphosphate (GTP), L-glutamic acid and p-aminobenzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as cobalamines and especially Bi2 vitamins) and porphyrins belong to a group of chemicals characterized by a tetrapyrol ring system. The biosynthesis of vitamin B-_; it is sufficiently complex that it has not been fully characterized to date but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate) nicotinamine are peridin derivatives that are also known as "niacin". Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms. The large-scale production of these compounds is based largely on chemical synthesis without cells, even though some of these chemical agents have also been produced by large-scale cultivation of microorganisms such as riboflavin, vitamin B, pantothenate and biotin. Only B; 2 is produced only by fermentation, due to the complexity of its synthesis. In vi tro methodologies require significant contributions in terms of materials and time, often at a high cost. C. Metabolism of purine, pinmidine, nucleoside and nucleotides, and uses The genes for the metabolism of purine and pyrimidine and their corresponding proteins are important in the therapy of tumor diseases and viral infections. The terms "purine" or "pyrimidine" include the nitrogenous bases that constitute the nucleic acids, coenzymes and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which consist of a nitrogenous base, a pentose sugar (in the case of RNA, sugar is ribose, in the case of DNA, sugar is D-deoxyribose) , and phosphoric acid. The term "Nucleosides" includes molecules that serve as precursors for nucleotides, but do not have the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit the synthesis ie RNA and DNA; by inhibiting this activity in a targeted manner towards cancer cells, the ability of tumor cells to divide and replicate can be inhibited. In addition, there are nucleotides that do not form nucleic acid molecules, but serve as energy stores (ie AMP) or as coenzymes (ie FAD and NAD) Several publications have described the use of these chemical agents for these medical indications. , through the influence of purine and / or pyrimidine metabolism (eg Christopherson, RI and Lyons, SD 1990) "Potent inhibitors of de novo pyrimidine and purine ciosynthesis as chemotherapeutic agents." Med. Res. Revi ews 10: 505-548 ). Studies of enzymes involved in the metabolism of purine and pyrimidine have been focused on the development of new drugs that can be used, for example, as immunosuppressants or anti-proliferation agents (Smith, JL, (1995) "Enzymes in nucleotide synthesis. ^ Curr. Opin Struct. Biol. 5: 752-757; (1995) Bi ochem Soc. Transact. 23: 877-902.) However, the purine and pyrimidine, nucleoside and nucleotide bases have other utilities: as intermediates in the biosynthesis of several fine chemicals (such as, for example, thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy vehicles for the cell (for example, ATP or GMP) and for chemical agents themselves, commonly employed as flavor enhancers (eg, IMP or GMP) or for various medical applications (see, eg, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol.6, Rehm et al., eds. : Weinheim, pages 561-612). The same enzymes involved in purine, pyrimidine, nucleoside or nucleotide metabolism increasingly serve as targets with which chemical agents are developed for the protection of crops, including fungicides, herbicides and insecticides. The metabolism of these compounds in bacteria has been characterized (for review see, for example, Zalkin, H. and Dixon, JE (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press: pages 259-287 and Michal, G. (1999) "Nucleotides and Nucleosides", chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). The metabolism of purine has been the subject of intense research and is essential for the normal functioning of the cell. An affected metabolism of the purine in higher animals can cause severe diseases such as gout. Purine nucleotides are synthesized from ribose-5'-phosphate, in the series of steps through the intermediate inosine-5'-phosphate (IMP), which results in the production of guanosine-5'-monophosphate ( GMP) or adenosine-5'-monophosphate (AMP), for which the triphosphate forms used as nucleotides are easily formed. These compounds are also used as energy stores in such a way that their degradation provides energy for many different biochemical processes in the cell. The biosynthesis of pyrimidine proceeds through the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. In turn UMP is converted to histidine-5'-triphosphate (CTP). The deoxy forms of all of these nucleotides are produced in a one-step derivatization reaction from the ribose diphosphate form of the nucleotide to the deoxyribose diphosphate form of the nucleotide. When phosphorylation is carried out, these molecules are able to participate in the synthesis of DNA. D. Trehalose metabolism and uses Trehalose consists of two molecules of glucose, linked in a bond to, a-l, l. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical industry, the cosmetics industry and the biotechnology industry (see, for example, Nishimoto et al. (1998), North American Patent No. 5,759,610, Singer, M.A. and Lindquist, S. (1998) Trends Biotech 16: 460-467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes of numerous microorganisms and is released naturally in the surrounding environment, from which it can be collected using methods known in the art. II. RESISTANCE TO DAMAGE CAUSED BY CHEMICALS, ENVIRONMENTAL STRESS, AND ANTIBIOTICS The production of fine chemicals is typically done through large-scale cultivation of bacteria developed to produce and secrete large quantities of these molecules. However, this type of large-scale fermentation results in the subjection of microorganisms to stress of various types. These stresses include environmental stress and chemical stress. A. Resistance to environmental stress Examples of environmental stresses typically found in large-scale fermentative culture include mechanical stress, thermal stress, stress caused by a limited amount of oxygen, stress caused by free radicals, pH stress, as well as osmotic stress. The agitation mechanism used in most large-scale fermenters to ensure crop ventilation produces heat, thus increasing the culture temperature. Increases in temperature induce the well-known thermal shock response where a group of proteins are expressed which not only help the survival of the bacteria against high temperatures but also increases survival in response to numerous other environmental stresses (see, Neidhardt, FC et al., Eds. (1996) E. coli and Salmonella, ASM Press: Washington, DC pp. 132-1399; Wosten, MM (1998) FEMS Microbiology Reviews 22 (3): 17-50; Bahl, H. et al. (1995) FEMS Microbiology Reviews 17 (3): 341-348; Zimmerman, JL, Cohill, PR (1991) New Biologist 3 (7): 641-650; Samali, A., and Orrenius, S. (1998) Cell. Stress Chaperones 3 (4): 228-236, and references contained therein from each of these quotes). The regulation of the response to heat shock in bacteria is facilitated by specific sigma factors and other cellular regulators of gene expression (Hecker, M., Volker, U (1998), Molecular Microbiology 29 (5): 1129-1136). One of the most important problems that the cell encounters when exposed to high temperature is that the folding of the protein is affected; Incipient proteins have sufficient kinetic energy under high temperature circumstances that it is difficult for the growing peptide chain to maintain a stable conformation for a sufficiently long period to be properly bent. Thus, two of the major types of proteins that are expressed during the heat shock response consists of chaperones (proteins that aid in the folding or unfolding of other proteins - see, for example, Fink, AL (1999) Physiol. Rev. 79 ( 2): 425-449), and proteases, which can destroy any inappropriately folded protein. Examples of chaperones expressed during heat shock responses include GroEL and DNAK; Proteases known to be expressed during this cellular reaction to thermal shock include Lon, FtsH and ClpB. Other environmental stresses other than heat can also cause a stress response. Even though the fermentor agitation process is intended to introduce oxygen to the crop, oxygen can remain in limited supply, especially when the crop is advanced in its growth and therefore the oxygen requirements of the crop are greater; an insufficient supply of oxygen is another stress for the microorganism. Cells in fermentor cultures are also subjected to numerous osmotic stresses, especially when nutrients are added to the culture, resulting in a high extracellular concentration and a low intracellular concentration of these molecules. In addition, the large amounts of the desired molecules produced by these organisms in culture can contribute to the osmotic stress of the bacteria. Finally, aerobic metabolism such as the metabolism used by C. gl utamxcum results in carbon dioxide as a waste product; The secretion of this molecule can acidify the culture medium due to the conversion of this molecule into carboxylic acid. Thus, bacteria in culture are also frequently subjected to acid pH stress. The opposite may also be true - when high levels of basic residual molecules such as ammonium are present in the culture medium, the bacteria in culture may be subject to a stress of basic pH as well. To combat such environmental stresses, bacteria have elegant gene systems that are expressed by being exposed to one or several stresses, such as the heat shock system mentioned above. Genes expressed in response to osmotic stress, for example, encode proteins capable of transporting or synthesizing compatible solutes in such a way that said absorption or osmotic export of a particular molecule is slower to reach manageable levels. Other examples of stress-induced bacterial proteins are the proteins involved in the biosynthesis of trehalose, the coding enzymes involved in ppGpp metabolism, the enzymes involved in signal transduction, especially the two-component coding systems sensitive to osmotic pressure, and the which encode transcription factors that respond to various stress factors (eg, RssB analogs and / or sigma factors). Many other genes of this type and their protein products are known in the art. B. Resistance to chemical stress Apart from environmental stresses, cells can also be subjected to numerous chemical stresses. These stress fall into two categories. The first category refers to natural residual products of metabolism and other cellular processes that are secreted by the cell into the surrounding environment. The second category refers to chemicals present in the extracellular medium that do not originate from the cell. In general, when the cells excrete toxic waste products from the concentrated intracellular cytoplasm in the relatively much more dilute extracellular medium, these products dissipate in such a way that the extracellular levels of the possibly toxic compound are very low. However, in large-scale fermentative culture of the bacteria, this may not be the case: so many bacteria are grown in a relatively small environment and at such a high metabolic rate that residual products can accumulate in the medium to almost toxic levels. Examples of such residues are carbon dioxide, metal ions, and reactive oxygen species such as hydrogene peroxide. These compounds can interfere with the activity or structure of cell surface molecules, or they can re-enter the cell, where they can seriously damage proteins and also nucleic acids. Other chemicals dangerous to the normal functioning of cells can be found in the extracellular environment. For example, metal ions such as mercury, cadmium, nickel or copper are frequently found in water sources, and can form narrow complexes with cellular enzymes that prevent the normal functioning of these proteins. C. Resistance to antibiotics Bacteriocidal proteins or antibiotics can also be found in the extracellular space, either through the intervention of the researcher or as a natural product of another organism, used to achieve a competitive sale. Microorganisms have several mechanisms known in the art to protect themselves from antimicrobial chemicals. The degradation, modification, and export of toxic compounds to the cell are common methods through which microorganisms eliminate antibiotics or remove their toxic character. Cytoplasmic "flow pumps" are known in several prokaryotes and show similarities with what are known as "multiple drug resistance" proteins from higher eukaryotes (Neyfakh, AA, et al. (1991) Proc. Nati. Acad. Sci. USA 88: 4781-4785). Examples of such proteins include emrAB from E. coli (Lomovskaya, O. and K. Lewis (1992) Proc.? Nati Acad. Sci. USA 89: 8938-8942), lmr? of B. subtilis (Ku ano, M. et al. (1997) Microbiology 143: 2775-2782), smr of S. aureus (Grinius, L.G. et al. (1992) Plasmid 27: 119-129) or c r of C. gl utamicum (Kaidoh, K. et al. (1997) Micro. Drug Resist., 3: 345-350). C. gl utamxcum e? yes nc is pathogenic, in contrast to several other members of the genus Corynebacterxum glutamicum, such as C. diphtheriae or C. pseudotuberculosis. Several pathogenic corynebacteria are known to have multiple resistance against several antibiotics, such as C. jexkex um and C. urealytx cum (Soriano, F. et al. (1995) Antimicrob Agents Chemot er.39: 208-214). Lincosamides are recognized as effective antibiotics against Corynebacterium species (Soriano, F. et al. (1995, Antimicrob Agents Chemother, 39: 208-214) .An unexpected result of the present invention was the identification of a gene encoding a resistance protein. to lincosamide (particularly, a lincomycin resistance protein) The LMRB protein from C. gl utamicum shows a homology of 40% with the product of the lmrB gene from B. subtilxs (see Genbank accession number AL009126 ), according to the calculation using the version 1.7 of the CLUSTALW program 'Thompson, JD, Higgins, DG, Gibson, TJ (1994) Nucí Acids Res. 22: 4673-4680) using standard parameters (TORQUE ALIGNMENT PARAMETERS: slow / precise alignments: Open Space Penalty = 10.00, Space Extension Penalty = 0.10, Protein Weight Matrix = BLOSUM 30, DNA Weight Matrix = IUB, Quick Alignments / Approximate: Space penalty = 3, K-tuple size (word) = 1, number of superior diagonals = 5, window size = 5, alignments in pairs Slow / Fast Displacement = slow. Parameters of multiple alignments: Aperture penalty of 'Space = 10. CJ, Space Extension Penalty = 0.05, divergent delay sequences = 40%, weight of DNA transitions = 0.50, protein weight matrix = BLOSUM series, matrix of DNA weight = IUB, negative use matrix = DISABLED). Environmental stress is chemical stress, and the stress of antibiotics or other antimicrobial agents can influence the behavior of microorganisms during fermentor culture, and can have an impact on the production of the desired compound from these organisms. For example, the osmotic stress of a microorganism can cause inappropriate or inappropriately rapid absorption of one or more compounds, which can ultimately cause cell damage or cell death due to an osmotic shock. Similarly, chemicals present in culture, either aggregated exogenously (e.g., antimicrobial compounds intended to kill unwanted microbes, or generated by the bacteria themselves, (e.g., residual compounds such as heavy metals or oxygen, or even antimicrobial compounds), can result in the inhibition of the production of fine chemicals or even the death of the organism The genes of the invention encode proteins of C. gl utamxcum that act to prevent cell damage or cell death , by specifically counteracting the source or effect of environmental or chemical stress III ELEMENTS AND METHODS OF THE INVENTION The present invention is based, at least in part, on the discovery of novel molecules, which are known herein as molecules of protein and nucleic acid of SR7, which increase the ability of C. gl utamicum to survive in chemical or environmentally dangerous. In one embodiment, the SRT molecules function to provide resistance to one or more environmental or chemical stresses for C. gl utamicum. In a preferred embodiment, the activity of the SRT molecules of the present invention have an impact on the production of a fine chemical desired by this organism. In a particularly preferred embodiment, the SRT molecules of the invention are modulated in activity in such a way that the yield, production, and / or production efficiency of one or several fine chemicals from C. gl utamx cum can also be modulated. The terms, "SRT protein" or "SRT polypeptide" include proteins that participate in the resistance of C. gl utamicum to one or more environmental or chemical stresses. Examples of SRT proteins include the proteins encoded by the SRT genes presented in Table 1 and by the SEQ ID NOs of the odd number. The terms "SRT gene" or "SRT nucleic acid sequence" include nucleic acid sequences encoding an SRT protein, which consist of a coding region and also regions of corresponding 5 'and 3' non-translated sequences. Examples of SRT genes include the genes presented in Table 1. The terms "production" or "productivity" are recognized in the art and include the concentration of the fermentation product (eg, the desired fine chemical) formed within a period of time. given time and a given volume of fermentation (for example, kg of product per hour per liter). The term "production efficiency" includes the time required to achieve a particular level of production (for example, the time needed for the cell to reach a particular rate of fine chemical production). The term "yield" or "product yield / carbon "is known in the art and includes the efficiency of the conversion of the carbon source into the product (ie, fine chemical." This is generally written, for example, kg of product or kg of carbon source. increase in the yield or production of the compound, the amount of molecules recovered, or of recovered molecules useful from this compound in a given amount of culture during a given period of time, is increased .The terms "biosynthesis" or a "biosynthetic pathway" are thermal sensors known in the art and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be r a highly regulated and multi-stage process. The terms "degradation" or a "degradation pathway" are known in the art and include the decomposition of a compound, preferably an organic compound, by a cell into degradation products in general terms, smaller or less complex molecules) into what can be a highly regulated and multi-stage process. The term "metabolism" is known in the art and includes all the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, (for example, the metabolism of an amino acid such as for example glycine) comprises the biosynthetic, global modification and degradation pathways in the cell that are related to this compound. The terms "resistance" and "tolerance" are known in the art and include the ability of a cell not to be affected by exposure to a chemical or an environment that would otherwise be detrimental to the normal functioning of those organisms. The terms "stress" or "danger" include factors that are detrimental to the normal functioning of cells such as C. gl utamxcum. Examples of stress include "chemical stress", where a cell is exposed to one or more chemicals that are harmful to the cell, and "environmental stress", where a cell is exposed to an environmental condition that is not a condition to which said cell has adaptation. Chemical stresses can be either natural metabolic waste products, for example, not limited to these examples, reactive oxygen species or carbon dioxide, or chemicals present -otherwise in the environment, including, but not limited to, these examples, heavy metal ions or bacteriocidal proteins such as antibiotics. Environmental stresses can be, not limited to these examples, temperatures outside the normal range, suboptimal availability of oxygen, osmotic pressures, or extremes of pH as for example. In another embodiment, the SRT molecules of the invention can modulate the production of a desired molecule, such as, for example, a fine chemical, in a microorganism such as, for example, C. gl utamxcum. Using recombinant genetic techniques, one or more SRT proteins of the invention can be manipulated in such a way that their function is modulated. The alteration of the activity of genes of response, resistance or tolerance to stress, in such a way that the cell increases its tolerance to one or several stresses can improve the capacity of said cell to grow and multiply under conditions of relative stress in the case of large-scale fermentor culture. For example, by overexpression or manipulation of a chaperone molecule induced by heat shock such that it has an optimized activity, the ability of the bacterium to correctly fold proteins against non-optimal temperature conditions can be increased. The fact of having a lower number of misfolded proteins (and possibly with erroneous or non-functional regulation) allows the cell to increase its capacity to function normally in such a culture, which in turn must provide an increased viability. This overall increase in the number of cells that have greater viability and activity in the culture should also result in an increase in yield, production and / or production efficiency of one or more desired fine chemicals, due at least to the relatively greater number of cells that produce these chemicals in the crop. The isolated nucleic acid sequences of the invention are located within the genome of a strain of Corynebacterx um gl utamxcum available from the American Type Culture Coliection, which received the designation ATCC 13032. The nucleotide sequence of the SRT DNAs of C. gl utamx cum isolates and the predicted amino acid sequences of the SRT proteins of C. gl utamicum are shown in the Sequence List as SEQ ID NOs of odd number and SEQ ID NOs of even number, respectively. Computer analyzes were carried out which classified and / or identified these nucleotide sequences as sequences that encode proteins of resistance and tolerance to chemical and environmental stress. The present invention also relates to proteins having an amino acid sequence that is substantially homologous to an amino acid sequence of the invention (for example, the sequence of a SEQ ID NO of even number from the Sequence List). As used herein, a protein having an amino acid sequence that is substantially homologous to a selected amino acid sequence has a level of homology of at least about 50% with the amino acid sequence selected, for example, the entire sequence of Selected amino acids. A protein having an amino acid sequence that is substantially homologous to a selected amino acid sequence may also have a homology level of at least about 50-60%, ie preference of at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and more preferably at least about 96%, 97%, 98%, 99% or more with the selected amino acid sequence. Ranges as well as identity values that are intermediate in relation to the values mentioned above (for example, 75% - &0% identity, 85-87% identity, 91-92% identity) are also contemplated within the framework of the present invention. For example, ranges of identiiai values employing a combination of any of the above mentioned values as upper and / or lower limit are contemplated within the framework of the present invention. The SRT proteins or biologically active portions or fragments of the same ie the present invention can provide resistance or tolerance to one or several chemical or environmental stresses, or they can have one or several of the activities presented in Table 1. Several aspects of the present invention are described with additional details in the following subsections. A. Isolated Nucleic Acid Molecules One aspect of the invention relates to isolated nucleic acid molecules encoding SRT polypeptides or biologically active portions thereof, as well as to nucleic acid fragments sufficient to be used as hybridization probes or as primers for identification or amplification of nucleic acid encoding SRT (for example, SRT DNA). As used herein, the term "nucleic acid molecule" includes DNA molecules (e.g., cDNA or genomic DNA) and RNA molecules (e.g., mRNA and DNA analogs or PNAs generated using nucleotide analogs. untranslated sequence located both at the 3 'end and the 5' end of the coding region of the gene: at least about 100 nucleotides of sequence upstream of the 3 'end of the coding region and at least 20 nucleotides of sequence downstream of the 3 'end of the coding region of the gene The nucleic acid molecule can be single stranded or double stranded, but referred to is AD? ie double stranded.An "isolated" nucleic acid molecule is a it is separated from other nucleic acid molecules present in the natural source of the nucleic acid.Preferably, an "isolated" nucleic acid is free of flanking sequence natur ally the nucleic acid (i.e., sequences located at the 5 'and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived, for example, in various embodiments, the nucleic acid molecule of isolated SRT can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb, c well 0. 1 kb of nucleotide sequences that naturally flank the nucleic acid molecule in the genomic DNA of the cell from which the nucleic acid is derived (for example, a cell of C. gl utamxcum). In addition, an "isolated" nucleic acid molecule, such as for example the DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemical agents when synthesized chemically A nucleic acid molecule of the present invention, for example, a nucleic acid molecule having a nucleotide sequence of a SEQ ID NO: odd number of the Sequence List, or a portion thereof, can be isolated using standard techniques of molecular biology and the sequence information provided here. For example, a C. gl utamx cum SRT DNA can be isolated from a C. gl utami cum library using all or a portion of one of the sequences SEQ ID NO: odd number from the Sequence List as a hybridization probe and standard hybridization techniques (eg, as described in Sambrook, J., Fritsh, EF and Maniatis, T. Molecular Cloning, A Laboratory Manual 2nd, Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989). In addition, a nucleic acid molecule that encompasses all or a portion of one of the nucleic acid sequences of the invention (eg, a SEQ ID NO of odd number from the Sequence Listing) can be isolated by chain reaction of polymerase using initiators of oligonucleotides initiated on the basis of this sequence (for example, the nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention (for example, a SEQ ID NO of an odd number of the Sequence List) can be isolated by polymerase chain reaction using oligonucleotide primers designed based on said sequence). For example, mRNA can be isolated from normal enyelone cells (for example by the guanidinium thiocyanate extraction method of Chirgwin et al. (1979 ^ Biochemistry 18: 5294-5299) and the DNA can be prepared using reverse transcriptase (e.g. Moloney MLV reverse transcriptase available from Gibco / BRL, Bethesda, MD or AMV reverse transcriptase available from Seikagaku America, Inc., St Petersburg, FL.) Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based on one of the nucleotide sequences shown in the Sequence Listing A nucleic acid of the invention can be amplified using cDNA or alternatively genomic DNA as annealed and appropriate oligonucleotide primers in accordance with standard polymerase chain reaction amplification techniques. The nucleic acid amplified in this way can be cloned into a vector appropriate and characterized by DNA sequence analysis. In addition, oligonucleotides corresponding to a nucleotide sequence of SRT can be prepared by standard synthetic techniques, for example, using an automated DNA synthesizer. In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence List. The nucleic acid sequences of the invention, in accordance with that presented in the Sequence Listing, correspond to the SRT DNAs of Corynebacterium um gl utamicum of the invention. This DNA comprises sequences that encode SRT proteins (ie, the "coding region", indicated in each SEQ ID NO sequence of odd number in Sequence List) as well as the 5 'untranslated sequences and 3' untranslated sequences, indicated also in each SEQ ID NO: odd number in the Sequence List. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the nucleic acid sequences of the Sequence List. For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences presented in the Sequence List has an identification number RXA, RXN or RXS having the designation "RXA", "RXN" or "RXS" "followed by 5 digits (for example RXA01524, RXN00493, or RXS 01027). Each of the nucleic acid sequences comprises up to three parts: an upstream region of 5 ', a coding region, and a downstream region. Each of these three regions is identified by the same designation RXA, RXN, or RXS to eliminate confusion. The expression "one of the odd number sequences of the Sequence List", then refers to any of the nucleic acid sequences in the Sequence List, which may also be distinguished by their different RXA, RXN, or RXS designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also presented in the Sequence List, as an even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence. For example, the coding region for RXA01524 is presented in SEQ ID NO: 1, while the amino acid sequence encoding it is presented as SEQ ID NO: 2. The sequences of the nucleic acid molecules of the invention are identified by the same designations RXA, RXN or RXS as the amino acid molecules they encode, in such a way that they can be easily correlated. For example, the amino acid sequence designated PXi01524 is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule, RXA01524, the amino acid sequence designated RXN00034 is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule RXN00034, and the amino acid sequence designated RXS00568 is a translation of the coding region of the nucleotide sequence of the nucleic acid molecule RXS00568. The correspondence between the nucleotide and amino acid sequences of RXA, RXN and RXS of the invention and their assigned SEQ ID NOs is presented in Table 1. Several of the genes of the invention are "genes designated with the letter F". A gene designated with the letter F includes the genes presented in Table 1 having a letter "F" in front of the designation RXA, RXN or RXS. For example, SEQ ID NO: 7, designated, as indicated in Table 1, as "F RXA00498", is a gene designated with letter F, as are SEQ ID NOs: 25, 33, and 37 (designated in the Table 1 as "FRXA01345", "FRXA02543", and "FRXA02282", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not contemplated as including the molecules compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, A., et al. (1998) J. Bacteriol. 180 (12); 3159-3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version was based on an incorrect initial codon, and therefore represents only a fragment of the actual coding region. In another preferred embodiment, an isolated nucleic acid molecule of the present invention comprises a nucleic acid molecule that is a complement to one of the nucleotide sequences of the invention (e.g., a sequence of a SEQ ID NO: odd of the Sequence List) or a part of it. A nucleic acid molecule that is complementary to one of the nucleotide sequences of the invention is a molecule that is sufficiently complementary to one of the nucleotide sequences shown in the Sequence List (e.g., the sequence of a SEQ ID NO: of odd number) in such a way that it can hybridize with one of the nucleotide sequences of the invention, thereby forming a stable duplex. In another preferred embodiment of the invention, an isolated nucleic acid molecule of the present invention comprises a nucleotide sequence having a homology level of at least about 50%, 51%, 52%, 53%, 54%, 55 %, 56%, 57%, 58%, 59% or 60%, preferably at least approximately 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73% 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89% or 90%, or 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more with a nucleotide sequence of the invention (for example a sequence of SEQ ID NO: odd number of the Sequence List) or a part thereof. Intermediate identity ranges and values in relation to the ranges mentioned above (an identity level of 70-90% or 80-95%) are also contemplated within the framework of the present invention for example ranges of identity values which use a combination of any of the values mentioned above as upper and / or lower limits are also included. In a further preferred embodiment an isolated nucleic acid molecule of the invention comprises a nucleotide sequence that hybridizes, for example, hybridizes under stringent conditions to one of the nucleotide sequences of the invention, or a portion thereof. In addition, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of SEQ ID NOs of odd number from the Sequence List, eg, a fragment that can be used as a probe or initiator or a fragment encoding a biologically active portion of an SRT protein. The nucleotide sequences determined from the cloning of the SRT genes from C. glutamx cum into the generation of probes and primers designed to be used to identify and / or clone SRT homologs in other cell types and organisms, as well as SRT homologs from other Corinebacteria or related species. Probes / primers typically comprise substantially purified oligonucleotides. The oligonucleotide typically comprises a nucleotide sequence region that hybridizes under stringent conditions to at least about 12, preferably about 25, more preferably, about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention (for example, a sequence of one of the SEQ ID NOs of odd number of the Sequence List), an antisense sequence of one of these sequences, or imitations that occur naturally from them. Initiators based on a nucleotide sequence of the invention can be used in polymerase chain reactions to clone SRT homologues. They are the passages in the nucleotide sequences of SRT can be used to detect transcripts or genomic sequences that encode the same protein or a homologous protein. In preferred embodiments, the probe further comprises a label group attached thereto as for example, the label group can be a radioisotope, a fluorescent compound, an enzyme, an enzyme cofactor. Such probes can be used as part of a set of diagnostic test elements to identify cells that erroneously express a SRT protein as for example by measuring a level of nucleic acid encoding SRT in a sample of cells, such as for example , the detection of SRT mRNA levels or the determination of whether a genomic SRT gene has been mutated or removed. In one embodiment, the nucleic acid molecule of the present invention encodes a protein or part thereof that includes an amino acid sequence that is sufficiently homologous with an amino acid sequence of the invention (eg, a sequence of a SEQ ID NO. odd number of the Sequence List) in such a way that the protein or portion thereof retains the ability to provide resistance or tolerance of C. gl utamxcum to one or more chemical or environmental stresses. As used herein, the term "sufficiently homologous" refers to proteins or portions thereof that have amino acid sequences that include a minimum number of identical or equivalent amino acid residues (e.g., an amino acid residue having a side chain) similar to an amino acid residue in a sequence of one or several of the even number SEQ ID NOs of the Sequence List) with an amino acid sequence of the invention such that the protein or portion thereof may participate in the C. gl utamx resistance to one or several chemical or environmental stresses. Proteins that are members of such metabolic pathways, in accordance with what is described herein, function to increase the resistance or tolerance of C. gl utami cum to one or several environmental or chemical hazards or stresses. Examples of such activities are also described here. Thus, "the function of an SRT protein" contributes to the global resistance of C. gl utamicum to elements of its environment that may impede its growth or normal functioning, and / or contribute, either directly or indirectly, to yield, production , and / or production efficiency of one or several fine chemicals. Examples of SRT protein activities are presented in Table 1. In another embodiment, the protein has a homology level of at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and more preferably at least about 96%, 97%, 98%, 99% or more with an entire amino acid sequence of the invention (for example, a sequence of a SEQ ID NO: of even number from the Sequence List). Intermediate identity ranges and values in relation to the values mentioned above (for example, an identity level of 75% -80%, an identity level of 85-87%, or an identity level of 91-92%) is also within the scope of the present invention. For example, ranges of identity values employing a combination of the aforementioned values indicated as upper and / or lower limits are also contemplated within the framework of the present invention. Portions of proteins encoded by the SRT nucleic acid molecules of the invention are preferably biologically active portions of one of the SRT proteins. As used herein, the term "biologically active portion of an SRT protein" includes a portion, eg, a domain / motif, of a SRT protein that can provide resistance or tolerance to one or more environmental or chemical hazards or stresses. , or it has an activity in accordance with the provisions in Table 1. To determine if an SRT protein or a biologically active portion of it can increase the resistance or tolerance of C. gl utamicum to one or more chemical hazards or stress or environmental, an enzyme activity assay can be performed. Such test methods are well known to those of ordinary skill in the art, in accordance with what is presented in detail in Example 8 of the Examples Section. Additional fragments of nucleic acids encoding biologically active portions of an SRT protein can be prepared by isolating a portion of one of the amino acid sequences of the invention (e.g., a sequence of an SEQ ID NO: Sequence List), which expresses the encoded portion of the SRT protein or peptide (for example, by recombinant expression in vitro) and by evaluating the activity of the encoded portion of the SRT protein or peptide. The invention also encompasses nucleic acid molecules that differ from one of the nucleotide sequences of the invention (eg, a sequence of an SEQ ID NO: odd number from the Sequence List) (and portions thereof) due to the degeneracy of the genetic code and therefore encode the same SRT protein as the protein encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the present invention has a nucleotide sequence that encodes a protein having an amino acid sequence shown in the Sequence List (eg, an even-numbered SEQ ID NO:). In a further embodiment, the nucleic acid molecule of the invention encodes a full-length C. gl utamxcum protein substantially homologous with an amino acid sequence of the invention (encoded by an open reading frame shown in a SEQ ID NO: of odd number from the Sequence List). It will be understood by a person with ordinary skill in the art that, in one embodiment, the sequences of the invention are not intended to include the prior art sequences such as the Genbank sequences presented in Tables 2 or 4 available prior to publication. present invention. In one embodiment, the invention includes nucleotide or amino acid sequences having a percent identity with a nucleotide or amino acid sequence of the invention that is greater than the percent identity of a prior art sequence (a Genbank sequence (or protein encoded by said sequence) presented in Tables 2 or 4). For example, the invention includes a sequence of nuclectides having a level of identity that is greater than or equal to at least 39% with the nucleotide sequence designated RXA00084 (SEQ ID NO: 189), a nucleotide sequence representing a level of identity that is greater or at least equal to 56% relative to the nucleotide sequence designated RXA00605 (SEQ ID NO: 11), and a nucleotide sequence having an identity level that is greater than 50% or at least equal to 50%, relative to the nucleotide sequence designated RXA00886 (SEQ ID NO: 39). A person with ordinary knowledge in the art will be able to calculate the lower percent identity limit for any given sequence of the invention by examining the percentage identity scores calculated by GAP presented in Table 4 for each of the three superior results for the given sequence, and subtracting from 100% the percentage identity calculated by higher GAP. A person with ordinary knowledge in the field will recognize that nucleic acid and amino acid sequences that have percentages higher than the lower limits calculated in this way (a level of identity of at least 50%, 51%, 52%, 53% , 54%, 55%, 56%, 57%, 58%, 59% or 60%, preferably at least 61%, 62%, 63% 64%, 65%, 66%, 67%, 68% , 69% or 70%, with greater preference of at least approximately 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 56%, 8%, 88%, 89% or 90% or 91%, 92%, 93%, 94%, and even greater preference, 95% 96%, 97%, 98%, 99% or more are also within the scope of the present invention.

In addition to the nucleotide sequences of SRT of C. gl utamxcum presented in the Sequence List as SEQ ID NOs, of odd number, it will be observed by a person with ordinary knowledge in the field that polymorphisms of DNA sequences that cause changes in the amino acid sequences of SRT proteins may exist within a population (eg, the population of C. gl utamx cum). Such genetic polymorphism in the SRT gene may exist between individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding a SRT protein, preferably a SRT protein of C. gl utamxcum. Such natural variations can typically result in a 1-5% variation in the nucleotide sequence of the SRT gene. Each and every one of these variations of nucleotides and amino acid polymorphisms resulting in SRT that are the result of natural variation and that do not alter the functional activity of SRT proteins are contemplated within the framework of the present invention. Nucleic acid molecules corresponding to natural variants and non-C. gi zamxcum homologs of the C. gl utamx SRT DNA of the invention can be isolated on the basis of their homology with the nucleic acid of SRT of C. gl utamxcum disclosed herein. using the AZ, from C. gl uz amx cum c a part thereof, as a hybridization shadow in accordance with standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention has a length of at least 15 nucleotides and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of a SEQ ID NO: Odd number of the Sequence List. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein the expression "hybridizes under stringent conditions" is intended to describe hybridization and washing conditions under which sequences having a homology of at least 60% between them typically remain hybridized to each other. Preferably, the conditions are such that sequences exhibiting at least about 65% homology, more preferably at least about 70% homology, and preferably greater sequences exhibiting at least 75% homology between them remain typically hybridized between them. Such stringent conditions are known to those of ordinary skill in the art and can be found in Ausubel and Colleagues, Current Protocols in Molecular Biology, John Wiley & Sons. N.Y. (1989), 6.3.1-6.3.6. A preferred non-limiting example ie stringent hybridization conditions are hybridization in 6X sodium chloride / sodium citrate (SSC) at a temperature of about 45 ° C followed by one or more washes in 0.2 X SSC, 0.1% SDS at a temperature 50-65 ° C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the present invention corresponds to a naturally occurring nucleic acid molecule. As used herein, a naturally occurring nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (eg, encodes a natural protein). In one embodiment, the nucleic acid encodes a SRT protein of natural C. gl utamxcum. In addition to naturally occurring variants of the SRT sequence that may exist in the population, a person of ordinary skill in the art can observe that changes can be introduced by mutation in a nucleotide sequence of the invention, thus causing changes in the amino acid sequence of the encoded SRT protein, without altering the functional capacity of the SRT protein. For example, nucleotide substitutions that cause amino acid substitutions in "essential nc" amino acid residues can be made in a sequence of nucieetides of the invention. A non-essential amino acid residue is a residue that can be altered from a wild-type sequence of one of the SRT proteins (eg, a SEQ ID NO: of pair number from the Sequence List) without altering the activity of said SRT protein, while an "essential" amino acid residue is required for SRT protein activity. Other amino acid residues (however, for example those that are not conserved or only semi-conserved in the domain having an SRT activity) may not be essential for activity and therefore probably lend themselves to alteration without altering the activity of SRT. Accordingly, another aspect relates to nucleic acid molecules that encode SRT proteins that contain changes in amino acid residues that are not essential for SRT activity. Such SRT proteins differ in amino acid sequences from a sequence of a SEQ ID NO: of even number from the Sequence List and yet retain at least one of the SRT activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence having a homology of at least about 50% with an amino acid sequence of the invention and can increase the resistance c tolerance of C. gl utamicum to one or several environmental or chemical stresses, or have one or more of the activities presented in Table 1. Preferably, the prctein encoded by the nucleic acid molecule has a homology of at least about 50-60% with the amino acid sequence of one of the SEQ ID numbers of even number of the Sequence List, more preferably a homology of at least about 60-70% with one of these sequences, with even greater preference a homology of at least about 70-80%, 80-90%, 90-95% with one of these sequences, and most especially at least most 96%, 97%, 98%, or 99% homology with one of the amino acid sequences of the invention. To determine the percentage of homology of two amino acid sequences (for example, one of the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes (can be introduce spaces in the sequence of a protein or nucleic acid for optimal alignment with the other nucleic acid protein.) The amino acid residues or nucleotides at corresponding or posicicher amino acid positions are corresponding nucleotides are then compared. sequence (e.g. an ae of the amino acid sequences of the invention; is occupied by the same amino acid or nucleotide residue as the corresponding position in the other sequence (for example a mutant form of the amino acid sequence) then the molecules are homologous in this position (ie, in accordance with what is used here, the "homology" of amino acid or nucleic acid is equivalent to "amino acid or nucleic acid identity" the homology between the two sequences depends on the number of identical positions shared by the sequences (ie, the percentage of 10 homology = number of identical positions / total number of positions xlOO). An isolated nucleic acid molecule encoding an SRT protein homologous to a protein sequence of the invention (eg, a sequence of a SEQ ID JO: 15 even number of the Sequence List) can be created by introducing one or more substitutions, additions or deletions of nucleotides in a sequence of fl nucleotides of the invention in such a way that one or more amino acid substitutions, additions or deletions are 20 introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as by e; site-directed mutagenesis and mutagenesis mediated by polymerase chain reaction. Preferably, Conservative amino acid substitutions are made in one or more predicted nonessential amino acid residues. A conservative substitution of amino acids is a substitution in which the amino acid residue is replaced by an amino acid residue having a similar side chain. Families of amino acid residues that have similar side chains have been defined in the art. These families include amino acids with basic side chains (lysine, arginine, histidine) acid side chains (such as aspartic acid, glutamic acid) uncharged polar side chains (eg glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine) , non-polar side chains (eg alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan) beta-branched side chains (eg, threonine, valine, isoleucine), and aromatic side chains (eg, tyrosine, phenylalanine , tryptophan, histidine). Thus, a non-essential amino acid residue predicted in SRT protein is preferably replaced by another amino acid residue of the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly over all or a portion of an SRT coding sequence, such as by saturation mutagenesis, and the resulting mutants can be screened to determine an SRT activity described herein for identify mutants that preserve the activity of SRT. After the mutagenesis of one of the nucleotide sequences of one of SEQ ID Nos. With odd number of the Sequence List, the encoded protein can be expressed recombinantly and the activity of the protein can be determined, using, for example, tests described here (see Example 8 of the Examples Section). In addition to the nucleic acid molecules encoding SRT proteins described above, another aspect of the present invention relates to isolated nucleic acid molecules that are antisense. An "antisense" nucleic acid comprises a nucleotide sequence that is complementary to an antisense nucleic acid encoding a protein, for example, complementary to the coding strand of a double-stranded DNA molecule or complementary to an mRNA. Accordingly, an antisense nucleic acid can be linked by hydrogen to a sense nucleic acid. The antisense nucleic acid may be complementary to a complete coding chain SRT, or only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense relative to a "coding region" ie the coding strand of a nucleotide sequence encoding an SRT protein. The term "coding region" refers to the region ie the nucleotide sequence comprising codons that are translated into amino acid residues (for example, the entire coding region of SEQ ID NO: 120 (RXA00600) comprises nucleotides 1 to 1098) . In another embodiment, the antisense nucleic acid molecule is antisense relative to a "non-coding region" of the coding strand of a nucleotide sequence encoding SRT. The term "non-coding region" refers to the 5 'and 3' sequences that flank the coding region that are not translated into amino acids (i.e., is also known as untranslated regions 5 'and 3'). Given the coding strand sequences encoding SRT disclosed herein (eg, the sequences presented as SEQ ID NOs of even number in the Sequence Listing), antisense nucleic acids of the invention can be designed in accordance with the mating rules of bases of Watson and Crick. The antisense nucleic acid molecule may be complementary to the entire coding region of SRT mRNA, but more preferably it is an oligonucleotide that is antisense only relative to a portion of the coding or non-coding region of SRT mRNA. For example, the antisense oligonucleotide may be complementary to the region surrounding the translation initiation site of SRT mRNA. An antisense oligonucleotide can have, for example, about 5, 10, 15, 2., 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid (eg, an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or modified nucleotides ie various forms designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, for example phosphorothiate derivatives and nucleotides substituted by acridine can be used. Examples of modified nucleotides that can be used to generate the antisense nucleic acid include 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytokine, 5- (carboxyhydroxymethyl) uracil, 5-carboxylmethylaminomethyl- 2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyiadenine, 2-methygiguanine, 3-rr? Etiicitocin, 5-Methylcycline, N6-adenine, 7-methylguanine, 5-methylaminomethyl-methyluracil, 5-methoxyamine-emethi-2-thiouracil, beta-D-mannosylkeosine, 5'-methoxycarbonylmethyluracyl, 5-methoxyuracil, 2-methylthio-N-isopentenyladenine, uracil acid -5-oxyacetic acid (v), wibutoxosin, pseudouracil, keosin, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiuracil, 5-methyluracil, methyl ether of uracil-5-oxyacetic acid, uracil acid -5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3- (3-amino-3-N-2-carboxy propyl) uracil, (acp3) w, and 2,6-diaminopurine. Alternatively, the sense nucleic acid can be produced biologically by employing an expression vector in which a nucleic acid has been subcloned in an antisense orientation (ie, the RNA transcribed from the inserted nucleic acid will be of an antisense orientation with related to a target nucleic acid of interest, which is further described in the following subsection). The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ in such a way that they hybridize or one with cellular mRNA and / or AD? genomic encoding a SRT protein in order to inhibit the expression of the protein in this way, for example, by inhibiting transcription and / or translation. Hybridization can be carried out by conventional nucleotide complementarity in order to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule that binds with duplex ie AD ?, through specific interactions in the greater crack of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, for example, by linking the antisense nucleic acid molecule with a peptide or an antibody that binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be administered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs are referred in which the antisense nucleic acid molecule is placed under the control of a prokaryotic promoter., viral or eukaryotic strong. In another embodiment, the antisense nucleic acid molecule of the invention is an α-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA where, unlike the usual β-units, the chains are parallel to each other (Gaultier et al (1987) Nucleic Acids Res. 15: 6625- 6641). The antisense nucleic acid molecule can also comprise a 2'-c-methylribonucleotide (Inoue et al (1987), Nucleic Acid Res 15: 6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. 1998) FEBS Lett . 215: 327-330).

In another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity that can dissociate a single-stranded nucleic acid such as mRNA, with which they have a complementary region. Thus, ribozymes, for example hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334: 585-591)) can be used to catalytically dissociate transcripts from SRT mRNA in order to inhibit the translation of AR? M from SRT. A ribozyme having specificity for a nucleic acid encoding SRT can be designed based on the nucleotide sequence of AD? of SRT disclosed herein (ie SEQ ID? O: 119 (RXA00600)). For example, a derivative of an AR? of Tetrahymena L-l9 IVS can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be dissociated in an AR? m encoding SRT. See, for example, Cech et al. US Patent No. 4, 98", 071 and Cech et al, US Patent No. 5,116,742 Alternatively, SRT mRNA can be used to select a catalytic RNA having specific ribonuclease activity from a set of RNA molecules. for example, Bartel, D and Szostak, JW (1993) Science 261: 1411-1418 Alternatively, the expression of the SRT gene can be inhibited by the approach of nucleic acid sequences complementary to the regulatory region of a nucleotide sequence of SRT. (for example, a promoter and / or SRT enhancers) to form triple helical structures that prevent transcription of a SRT gene in target cells.See, broadly Helene, C. (1991) Anticancer Drug Des. 6 (6 ): 569-84; Helene C. Et al (1992) Ann NY Acad Sci. 660: 27-36; and Maher, LJ (992) Bioassays 14 (12): 807-15 B. Recombinant expression vectors and cells guests Another aspect of the invention relates to vectors, preferably expression vectors containing a nucleic acid encoding a SRT protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule that can carry another nucleic acid to which it is attached. One type of vector is a "plasmid," which refers to a circular double-stranded DNA loop in which additional segments of DNA can be ligated. Another type of vector is a viral vector in which additional segments of DNA can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (eg, bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the nuéspei cell, and therefore, are replicated together with the host genome. In addition, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are known herein as "expression vectors" in general, expression vectors useful in recombinant DNA techniques are often in the form of plasmids. In the present specification, the terms "plasmids" and "vector" can be used interchangeably since the plasmid is the most commonly used vector form. However, the invention is intended to include all other forms of expression vectors such as viral vectors (e.g., retroviruses, adenoviruses and adeno-associated viruses defective for replication), serve equivalent functions. The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences., selected based on the host cells to be used for expression, operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, the term "operably linked" is intended to indicate that the nucleotide sequence of interest is linked to the regulatory sequence (s) in a manner that allows expression of the nucleotide sequence (for example, in a transcription system of an in vitro translation or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology; Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include sequences that direct constitutive expression of a nucleotide sequence in many types of host cells and sequences that direct the expression of the nucleotide sequence only in certain host cells. Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, lpp-, lac-, lpp-lac-, lacl ^ -, T7-, T5-, T3 -, gal-, trc-, ara-, SP6-, arny, SP02,? -PR- or? PL / which are preferably used in bacteria. Additional regulatory sequences, for example, are promoters from yeasts and fungi such as ADC1, MFa, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMv / 355, SSU , OCS, lib4, usp, STLS1, B33, or ubiquitin or phaseolin promoters. It is also possible to use artificial promoters. A person with ordinary knowledge in the art will note that the design of the expression vector may depend on such factors as the choice of the host cell to be transformed, the desired level of protein expression. The expression vectors of the invention can be introduced into the host cells in order to thereby produce proteins or peptides, including proteins or fusion peptides, which are encoded by nucleic acids in accordance with that described herein, for example, proteins. of SRT, form SRT protein mutants, fusion proteins, etc. The recombinant expression vectors of the invention can be designed for expression of SRT proteins in prokaryotic or eukaryotic cells. For example, SRT genes can be expressed in bacterial cells such as C. gl utamxcum, insect cells (using baculovirus expression vectors) yeast and other fungal cells (see Romans MA et al. (1992) "Foreign gene expression in yeast: a review ", Yeast 8: 423-488, van den Hondel CAMJJ et al. (1991)" Heterologous gene expression in filamentous fungi "in: More Gene Manipulations in Fungi, JW Bennet &LL Lasure, eds., pages 396-428: Academic Press: San Diego; and van den Hondel, CAMJJ &Punt, PJ (1991) "Gene transfer systems and vector development for fiiamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, JF et al., eds. pages 1-28, Cambridge University Press, Cambridge), algae and multicellular plant cells (see Schmidt, R. And Willmitzer, L. (1988) High efficiency Agrobacterxum tumefaciens-transformation mediated by Arabidopsis thaliana leaf and cotyledon explants "Plant Cell Rep. 583-586) , or mammalian cells. Suitable host cells are further discussed in Goeddel, Gene Expression Technology Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example, using regulatory sequences of T7 promoter and T7 polymerase. The expression of proteins in prokaryotes is most often effected with vectors containing constitutive or inducible promoters that direct the expression of either fusion proteins or non-fusion proteins. Fusion vectors add several amino acids to an encoded protein, usually to the amino terminus of the recombinant protein. Such fusion vectors typically have three purposes: 1) to increase the expression of recombinant protein; 2) increase the solubility of the recombinant protein; and 3) helping to purify the recombinant protein by its action as a ligand in affinity purification. Frequently, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion portion and the recombinant protein in order to allow separation of the recombinant protein from the fusion portion after purification. of the fusion protein. Enzymes of this type and their recognition sequences include factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc., Smith D.B. and Johnson, K.S. (1988) Gene 67: 31-40); pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) that fuse glutathione S-transferase (GST) maltose binding protein E, or protein A respectively, on the target recombinant protein. In one embodiment, the coding sequence of the SRT protein is cloned into an expression vector of pGEX to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site - protein X. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin the recombinant SRT protein not fused to GST can be rered by dissociation of the fusion protein with thrombin. Examples of suitable non-inducible fusion vectors of E. coli include pTrc (Amann et al (1988) Gene 69: 301-315) pLG338, pACYC184, pBR322, 0UCI8, pUC19, pKC30, pRep4, pee, pHSl , pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN-III113-Bl,? gtll, pBdCl, and pET lid (Studier et al., Gene Expression Technology Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60 -89; and Pouwels et al., Eds. (1985) Cloning Vectors, Elsevier: New York IBSN 0 444 904018). The expression of target gene from pTrc vector is based on the transcription of host RNA polymerase from a trp-lac hybrid fusion promoter. The expression of the white gene from the vector pET lid is based on the transcription from the fusion promoter gn 10-lac of T7 mediated by an RA? viral co-expressed polymerase (T7 gnl). This viral polymerase is supplied by BL21 (DE3) or HMS17 (DE3) host strains from a prophase? resident that contains a gnl gene of T7 under the transcriptional control of the lacUV5 promoter. For the transformation of other varieties of bacteria, appropriate vectors can be selected. For example, plasmids pIJIOl, pIJ364, pIJ702 and pIJ361 are useful for transforming Streptomyces while plasmids pUBUO, pC194 or pBD214 are suitable for transforming Bacillus species. Several plasmids useful in the transfer of genetic information in Corynebacterium include pHM1519, pBLl, pSA ~~ or pAJ667 (Pouwels et al., Eds. (1985) Cloning Vectors Elsevier:? Ew York IBS? 0 444 904018). One strategy for optimizing the expression ie recombinant protein is to express the protein in a host bacterium with an affected capacity for proteoitic dissociation of the recombinant protein (Gottesman, S., Gene Expression Technology Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector such that the individual codons for each amino acid are the codons used preferably in the bacterium selected for expression, such as C. glutamxcum (Wada et al. (1992) Nucleic Acids Res. 20: 2111-2118). Said alteration of nucleic acid sequences of the invention can be carried out by standard techniques of DNA synthesis. In another embodiment, the SRT protein expression vector is a yeast expression vector. Examples of vectors for expression of S. Cerivisae yeast include pYepSecl (Baldari, et al., (1987) E bo J. 6: 229-234), 2 μ, pAG-1, Yepd, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, 1982) Cell 30: 933-943), pJRY88 (Schultz et al, (1987) Gene 54: 113-123) and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for construction of appropriate vectors for use in other fungi, such as filamentous fungi, include the vectors and methods presented in detail at: van den Hondel, C.A.M.J.J. & Punt, P.J. 1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied molecular Genetics of Fungi, J.F. Peberdy, et al., Eds., Pages 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elservier: New York (IBSN 0 444 904018). Alternatively, the SRT proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (for example SF9 cells) include the pAc series (Smith et al (1983) Mol Cell Biol. 3: 2156-2165) and the pVL series (Lucklow and Summers ( 1989) Virology 170: 31-39). In another embodiment, the SRT proteins of the invention can be expressed in cells of unicellular plants (such as algae) or in cells of higher plants (for example, spermatophytes, such as harvest plants). In another embodiment, the SRT proteins of the invention can be expressed in cells of unicellular plants (such as algae) or in cells of higher plants (for example, spermatophytes, such as harvest plants). Examples of plant expression vectors include the vectors presented in detail in: 3ecker, D., Ke per, E., Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border ". Plant Mol. Biol. 20: 1195-1197; and Bevan, M.

W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucí. Acid Res. 12: 8711-8721, and includes pLGV23, pGHlac +, pBIN19, pAK2004, and pDH51 (Pouwels et al., Eds. (1985) Cloning Vectors, Elsevier: New York IBSN 0 444 904018). In another embodiment, a nucleic acid of the present invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian vectors include pCDM8 (Seed, B. (1987) Nature 329: 840) and pMT2PC (Kaufman et al (1987) EMBO J. 6: 197-195). When employed in mammalian cells, the control functions of the expression vector are often provided by viral regulatory elements. For example, commonly used promoters are polyoma derivatives, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other expression systems suitable for both prokaryotic and eukaryotic cells, see chapters 16 and 17 of Sambrook, J., Fritsh, EF, and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbcr, NY, 1989. In another embodiment, the recombinant mammalian expression vector can direct the expression of the nucleic acid preferably of a particular cell type. (for example, tissue-specific regulatory elements are used to express the nucleic acid). Specific regulatory elements for fabrics are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (specific for liver; Pinkert et al (1987) Genes Dev. 1: 268-277), lymphoid-specific promoters (Caiame and Eaton (1988) Adv. Immunol 43: 235-275), in particular T cell promoters (Winoto and Baltimore (1989) EMBO J. 8: 729-733) and immunoglobulins (Banerji et al. (1983) Cell 33: 729-740; Queen and Baltimore (1983) Cell 33: 741-748), specific promoters for neurons (for example, the neurofilament promoter: Byrne and Ruddle (1989) PNAS 86: 5473-5477), pancreas-specific promoters (Ediund et al. '.1985 ) Science 230: 912-916), and mammary gland-specific promoters (e.g., whey promoter; U.S. Patent No. 4,873,316 and Application Publication European No. 264,166). Promoters regulated by the development are also included, for example, murine hox promoters (Kessel and Gruss (1990) Science 249: 374-379) and the promoter of < x-fetoprotein (Campes and Tilghman (1989) Genes Dev. 3: 537-546). The invention further provides a recombinant expression vector comprising a DNA molecule ie the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operably linked to a regulatory sequence in such a way as to allow the expression (by transcription of the DNA molecule) of an RNA molecule that is antisense relative to SRT mRNA. Regulatory sequences operably linked to a nucleic acid cloned in the antisense orientation can be selected which direct the continuous expression of the antisense RNA molecule in various cell types, for example viral promoters and / or enhancers, or regulatory sequences can be selected which direct tissue-specific or tissue-specific constitutive expression of antisense RNA. The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus wherein antisense nucleic acids are produced under the control of a high efficiency regulatory region whose activity can be determined by the type of cell into which the vector is introduced. For comments on the regulation of gene expression using antisense genes, see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews - Trenas in Genetics, Vol. 1 (1) 1986. Another aspect of The invention relates to host cells in which a recombinant vector of the invention has been introduced. The terms "host cell" and "recc binant host cell" are used interchangeably herein. It is understood that these terms refer not only to the particular cell in question but to the progeny or potential progeny of said cell. Since certain modifications can occur in subsequent generations due to either mutations or environmental influences, in fact said progeny may not be identical to the cell of origin, but it is still included within the scope of the term in accordance with what was used. here. A host cell can be any prokaryotic or eukaryotic cell. For example, an SRT protein can be expressed in bacterial cells such as C. gi utamx cum, insect cells, yeast or mammalian cells (eg Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to a person of ordinary skill in the art. Microorganisms related to Corynebacterium gl utamx cum that can conveniently be used as host cells for the protein and nucleic acid molecules of the invention are presented in Table 3. Vector DNA can be introduced into prokaryotic or eukaryotic cells through conventional techniques of transformation or transfection. As used herein the terms "transformation" and "transfection" refer to several known techniques for introducing foreign nucleic acid for example, linear DNA or APN, eg, a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector (eg, a plasmid, phage, phasmid, phagemid, transposon or other DNA) in a host cell, including co-precipitation with calcium chloride or calcium phosphate, DEAE-dextran-mediated transfection , lipofection or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals. For a stable transfection of mammalian cells it is known that, according to the expression vector and according to the transfection technique that is used, only a small fraction of cells can integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene encoding a selectable marker (e.g., resistance to antibiotics) is generally introduced into the host cells together with the gene of interest. Preferred selectable markers include markers that provide resistance to drugs, such as G418, hygromycin and methotrexate. A nucleic acid encoding a selectable marker can be introduced into a host cell in the same vector as a nucleic acid encoding an SRT protein or it can be introduced into a separate vector. Cells transfected stably with the introduced nucleic acid can be identified by drug selection (for example, cells that have the selectable marker gene incorporated will survive, while the other cells will die). To create a homologous recombinant microorganism, a vector is prepared that contains at least a portion of an SRT gene into which a removal has been introduced., addition or substitution to alter in this way, for example, functionally upset the SRT gene. Preferably, this SRT gene is a SRT gene of Corynebacterxum glummicum, but may be a homologue of a related bacterium or even a mammalian, yeast or insect source. In a preferred embodiment, the vector is designed such that homologous recombination must be effected, the endogenous SRT gene is functionally disrupted, (ie, it no longer encodes a functional protein; it is also known as a "knocked out" vector). Alternatively, the vector can be designed in such a way that, upon homologous recombination, the endogenous SRT gene is mutated or otherwise altered but continues to encode a functional protein for example, the upstream regulatory region can be altered for the purpose of to thereby alter the expression of the endogenous SRT protein). In the homologous recombination vector the altered portion of the SRT gene is flanked at its 5 'and 3' ends by additional nucleic acid of the SRT gene to allow homologous recombination to occur between the exogenous SRT gene carried by the vector and a endogenous SRT gene in a microorganism. The additional flanking SRT nucleic acid is of sufficient length to effect successful homologous recombination with the endogenous gene. Typically, several kilobases of flank DNA (both at the 5 'and 3' ends) are included in the vector (such as, for example, Thomas, KR, and Capecchi, MR (1987) Cell 51: 513 for a description of vectors of homologous recombination). The vector is introduced into a microorganism (for example, by electroporation) and cells in which the introduced SRT gene has recombined in a manner homologous to the endogenous SRT gene are selected, using known techniques. In another embodiment, recombinant microorganisms can be produced which contain selected systems that allow regulated expression of the introduced gene. For example, the inclusion of a SRT gene in a vector by placing it under the control of the lac operon allows the expression of the ie SRT gene only in the presence of IPTG. Said regulatory systems are well known in the art. In another embodiment, an endogenous SRT gene in the host cell is disrupted (as by example, by homologous recombination or other genetic means known in the art) such that the expression of its protein product does not occur. In another embodiment, an endogenous SRT gene is either introduced into a host cell by one or several inversions, deletions or point mutations, but it continues to encode a functional SRT protein. In another embodiment, one or more of the regulatory regions (e.g., a promoter, repressor or inducer) of a SRT gene in a microorganism has been altered (e.g., by removal, truncation, inversion or dot mutation) of such that the expression of the SRT gene is modulated. A person of ordinary skill in the art will observe that host cells containing more than one of the described SRT gene and protein modifications can be readily produced using the methods of the invention and are included within the scope of the present invention. A host cell of the invention, such as for example a prokaryotic or eukaryotic cell in culture, can be used to produce (ie, express) an SRT protein. Accordingly, the invention further provides methods for the production of SRT protein using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of the invention (in which a recombinant expression vector decodes an SRT protein has been introduced, or in whose genomes a gene encoding a protein has been introduced. of wild-type or altered SRT) in a suitable medium until the production of an SRT protein. In another embodiment, the method further comprises isolating SRT proteins from the medium or host cell. C. Isolated SRT proteins Another aspect of the invention relates to isolated SRT proteins, as well as biologically active portions thereof. An "isolated" or "purified" protein or a biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The term "substantially free of cellular material" includes preparations of SRT protein in which the protein is separated from cellular components of the cells where it is produced naturally or recombinantly. In one embodiment the expression "substantially free of cellular material" includes preparations of SRT protein having less than about 30% (dry weight) of non-PTS protein (also referred to as a "contamination protein"), more preferably less than about 20% non-PTS protein, with still greater preference, less than about 10% non-PTS protein, and most especially less than about 5% non-PTS protein. When the SRT protein or biologically active portion thereof is produced recombinantly, it is also preferred that it be substantially free of the culture medium, ie, that the culture medium represents less than about 20%, more preferably less than about 10%, and most especially less than about 5% of the volume of the protein preparation. The expression "substantially free of chemical precursors or other chemicals" includes preparations of SRT protein wherein the protein is separated from the chemical precursors and other chemicals involved in the synthesis of the protein. In one embodiment, the phrase "substantially free of chemical precursors or other chemicals" includes SRT protein preparations having less than about 30% (by dry weight) of chemical precursors or non-PTS chemicals, more preferably less than about 20% of chemical precursors or non-PTS chemicals, preferably still less than about 10% non-PTS chemical or chemical precursors, and most especially less than about 5% non-PTS chemical or chemical precursors. In preferred embodiments, isolated proteins or biologically active portions thereof do not have contaminating proteins from the same organism from which the SRT protein is derived. Typically, such proteins are produced by recombinant expression, for example, of an SRT protein of C. gl utamx cum in a microorganism, such as for example C. gl utamicum. An isolated SRT protein or a part thereof of the present invention can contribute to the resistance or tolerance of C. gl utamx cum to one or several chemical or environmental hazards or stress, or it has one or several of the activities presented in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence that is sufficiently homologous to an amino acid sequence of the invention (eg, a sequence of an even-numbered SEQ ID NO: of Sequences) that the protein or portion thereof retains the ability to mediate the resistance or tolerance of C. gl utamicum to one or more chemical or environmental hazards or stresses. The portion of the protein is preferably a biologically active portion in accordance with what is described herein. In another preferred embodiment, an SRT protein has an amino acid sequence presented as a SEQ ID NO: of even number from the Sequence List. In another preferred embodiment, the SRT protein has an amino acid sequence that is encoded by a nucleotide sequence that hybridizes, eg, hybridizes under stringent conditions, to a nucleotide sequence of the invention (eg, a sequence ie SEQ ID LJ: the odd number of the Sequence List). In another preferred embodiment, the SRT protein has an amino acid sequence that is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57 %, 58%, 59%, or 60%, with greater preference at least approximately 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69% or 70%, with senior preference at least approximately 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80%, 81%, 82%, 83%, 84%, 85% , 86%, 87%, 88%, 89% or 90%, or 91%, 92%, 93%, 94% and with very special preference at least approximately 95%, 96%, 97%, 98%, 99% or more homologous with one of the nucleic acid sequences of the invention, or a portion thereof. Intermediate ranges and values of identity in relation to the values mentioned above (for example, an identity of 70-90% or an identity of 80-95%) are also included within the framework of the present invention. For example, ranges of identity values that employ a combination of any of the aforementioned values as upper and / or lower limit are also included within the scope of the present invention. Preferred SRT proteins of the present invention also preferably have at least one of the SRT activities described herein. For example, a preferred SRT protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence that hybridizes, such as, for example, hybridizes under stringent conditions, to a nucleotide sequence of the invention, and that may increase the resistance or tolerance of C. glutamicum to one or more environmental or chemical stresses, or having one or more of the activities presented in Table 1. In other embodiments, the SRT protein is substantially homologous to an amino acid sequence of the invention (as, for example, a sequence of an SEQ ID NO: of even number of the Sequence List) and retains the functional activity of the protein of one of the amino acid sequences of the invention and nevertheless differs in the amino acid sequences due to a natural variation or mutagenesis, in accordance with what is written in detail in subsection I above. Accordingly, in another embodiment, the SRT protein is a protein comprising an amino acid sequence that has a homology of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56% 57%, 58%, 59% or 60%, preferably a homology of at least approximately 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68% 69% or 70% , more preferably a homology of at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89% or 90% or 91%, 92%, 93%, 94%, and even more preferably a homology of at least about 95%, 96%, 97%, 98%, 99% or more in relation to a whole amino acid sequence of the invention and having at least one of the SRT activities described herein. Intermediate ranges and values of identity in relation to the values mentioned above (for example an identity of 70-90% or an identity of 80-95%) are also within the scope of the present invention. For example, ranges of identity values that employ the combination of any of the above-mentioned values as upper and / or lower limits are within the scope of the present invention. In another embodiment, the invention relates to a full-length C. gl utami cum protein that is substantially homologous to a whole amino acid sequence of the invention. Biologically active portions of an SRT protein include peptides comprising amino acid sequences derived from the amino acid sequence of a SRT protein, for example, an amino acid sequence of an even number SEQ ID NO: SEQ. amino acid sequence of a protein homologous to the SRT protein, which include fewer amino acids than a full-length SRT protein or the full-length protein that is homologous to a SRT protein, and exhibit at least one activity of a SRT protein. Typically, biologically active portions of peptides, for example, peptides having, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an SRT protein. In addition, other biologically active portions, wherein other regions of the protein are removed, can be prepared by recombinant techniques and can be evaluated in relation to one or more of the activities described herein. Preferably, the biologically active portions of a SRT protein include one or more selected domains / motifs or portions thereof that have the biological activity. SRT proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (in accordance with that described above) the expression vector is introduced into a host cell (in accordance with that described above), and the SRT protein is expressed in the host cell. The SRT protein can then be isolated from the cells through an appropriate purification scheme using standard protein purification techniques. Alternatively to recombinant expression, a SRT protein, polypeptide, or peptide can be chemically synthesized using standard peptide synthesis techniques. In addition, a native SRT protein can be isolated from cells (for example, endothelial cells), for example using an anti-PTS antibody, which can be produced by standard techniques using an SRT protein or fragment thereof of this invention. The invention also offers chimeric or fusion SRT proteins. As used herein, a "chimeric" protein of SRT or a "fusion protein" of SRT comprises an SRT polypeptide operably linked to a non-PTS poiyptype. A "SRT polypeptide" refers to a polypeptide having an amino acid sequence that corresponds to SRT, while a "non-PTS polypeptide" refers to a polypeptide having an amino acid sequence that corresponds to a protein that is not substantially homologous to the SRT protein, such as, for example, a protein that is different from the SRT protein and that is derived from the same organism or from a different organism. Within the fusion protein, the term "operably linked" is intended to indicate that the SRT polypeptide and the non-PTS polypeptide are fused in frame with each other. The non-PTS polypeptide may be fused to the N-terminal or C-terminus of the SRT polypeptide. For example, in one embodiment, the fusion protein is a GST-PTS fusion protein in which the SRT sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the encoding of reccmblant SRT proteins.

In another embodiment, the fusion protein is an SRT protein that contains a heterologous signal sequence at its N-terminus. In certain host cells (e.g., mammalian host cells), the expression and / or secretion of an SRT protein they can be increased through the use of a heterologous signal sequence. Preferably, a SRT, chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments encoding the different polypeptide sequences are ligated together in a frame in accordance with conventional techniques, such as, for example, by use of terminals with flat ends or stepped ends for ligation, digestion with restriction enzyme to provide terminals appropriate, filling of cohesive ends as appropriate, treatment with alkaline phosphates to avoid undesirable binding, as well as enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, amplification by polymerase chain reaction of gene fragments can be carried out using anchor primers that provide complementary projections between two consecutive gene fragments that can be subsequently fused and reamplified to generate a chimeric gene sequence; eg, Current Protocols in Molecular Biology, Ausubel et al. John Wiley & Sons: 1992). In addition, many expression vectors are commercially available which already encode a fusion portion (eg, a GST polypeptide). A nucleic acid encoding an SRT can be cloned into said expression vector such that the fusion portion is bound in frame with the SRT protein. Counterparts of the SRT protein can be generated by mutagenesis, eg, discrete point mutation or truncation of the SRT protein. As described herein the term "homologs" refers to a variant form of the SRT protein that acts as an agonist or antagonist of the activity of the SRT protein. An SRT protein agonist can conserve substantially the same biological activities of the SRT protein or a subset of said biological activities. An SRT protein antagonist can inhibit one or more of the activities of the SRT protein form that occurs naturally, for example by competitively linking a downstream or upstream member of the SRT system that includes the SRT protein. Thus, the SRT protein of C. gl utamicum and homologs thereof of the present invention can increase the tolerance or resistance of C. gl utamxcum to one or more chemical or environmental stresses.

In an alternative embodiment, homologs of the SRT protein can be identified by screening combinatorial libraries of mutants, eg, truncated mutants, of the SRT protein for SRT protein agonist or antagonist activity. In one embodiment, a library composed of SRT variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a library composed of genes. A library composed of SRT variants can be produced, for example, by enzymatically linking a mixture of synthetic oligonucleotides into gene sequences in such a way that a degenerate set of potential SRT sequences can be expressed as individual polypeptides or, alternatively, as a set of longer fusion proteins (eg, for phage display) that contain SRT sequence set therein. There are several methods that can be used to produce libraries of potential SRT homologs from a sequence of degenerate oligonucleotides. A chemical synthesis of a degenerate gene sequence in an automatic DNA synthesizer can be performed and the synthetic gene is then ligated into an appropriate expression vector. The use of a degenerate set of genes allows the delivery, in a mixture of all the sequences that encode the desired gene ie potential sequences of SRT. Methods for synthesizing oligonucleotides are known in the art (see, for example, Narang, SA (1983) Tetrahedron 39: 3, Itakura et al (1984) Annu, Rev. Biochem 53: 323, Itakura et al. (1984) Science 198: 1056; Ike et al. (1983) Nucleic Acid Res. 11: 477. In addition, fragment libraries of the coding SRT protein can be employed to generate a varied population of SRT fragments for screening and subsequent selection of homologues. of a SRT protein In one embodiment a library of fragments of coding sequences can be generated by treating the double stranded fragment per polymerase chain reaction of a SRT coding sequence with a nuclease under conditions in which a cut occurs only about once per molecule, denaturing the double-stranded DNA, renaturing the DNA to form double-stranded DNA which can include sense / antisense pairs of different cut products, remove single chain portions of the reformed duplexes by treatment with nuclease Sl and ligate the resulting fragment library into an expression vector. Through this method an expression library encoding an N, C terminal and internal fragments of various sizes of the SRT protein can be derived. Several techniques are known to screen gene pro -ducts from combinatorial libraries made by dot or truncation mutations, and to screen cDNA library for gene products having a selected property. Such techniques are adaptable for rapid screening of gene libraries generated by the combinatorial mutagenesis of SRT homolog. The most widely used techniques that lend themselves to high throughput analysis, to screen large gene libraries, typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and the expression of the combinatorial genes under conditions in which the protection of a desired activity facilitates the isolation of the vector encoding the gene whose product was detected. Recursive assembly mutagenesis (REM), a new technique that increases the frequency of functional mutants in libraries, can be used in combination with screening assays to identify SRT homologues (Arkin and Yourvan (1992) PNAS 89: 7811-7815; Delgrave et al. (1993) Protein Engineering 6 (3): 327-331). ' In another embodiment, cell-based assays can be exploited to analyze a varied SRT library, using methods well known in the art. D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologous, fusion protein, primers, vectors and host cells described herein can be used in one or more of the following methods: identification of C. gl utamicum and related organisms; mapping of genomes of related organisms such as C. gl utamxcum; identification and location of sequences of interest of C. gl utami cum; evolution studies; determination of SRT protein regions that are required per function; modulation of an SRT protein activity; modulation of the activity of a SRT pathway; and modulating cell production of a desired compound, such as a fine chemical. The SRT nucleic acid molecules of the invention have various uses. First, they can be used to identify an organism as Corynebacterx um gl utamxcum or a closely related organism. Likewise, they can be used to identify the presence of C. gl utami cum or a closely related organism in a mixed population of microorganisms. The invention offers the nucleic acid sequences of several genes of C. gl utamicum, by probing DNA genome extracted from a culture of a single or mixed population of microorganisms under stringent conditions with a probe encompassing a region ie gene C. gl utamxcum that is unique to this organism, it can be determined if this organism is present. Although Corynebacterx um gl u tamx cum itself is not pathogenic, it is related to pathogenic species such as Corynebacterium um Diphtheriae. Corynebacteri um diphtheri ae is the causative agent of diphtheria, an acute, rapidly developing febrile infection that includes both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves a necrotic lesion of the epithelial cells; the bacilli secrete toxin which is disseminated throughout this lesion to susceptible tissues of the body. Degenerative changes caused by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenal, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have a high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the Independent States of the Former Soviet Union. A current epidemic of diphtheria in these last two regions resulted in the death of at least 5,000 people since 1990. In one embodiment, the invention offers a method for identifying the presence or activity of Corynebacterium um dxphtheriae in a subject, this method includes detection of one or more nucleic acid sequences or amino acids of the invention (eg, the sequences presented as SEQ ID NOs, odd or even, respectively, in the Sequence List) in a subject, thus detecting the presence or activity of Corynebacterxum dxphtherxae in the subject. C. gl utamxcum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. gl utami cum are homologous to C. dxphtheriae nucleic acid and protein molecules, and therefore can be used to detect C. diphtherxae in a subject. The nucleic acid and protein molecules of the invention can also serve as markers for specific regions of a genome. This is useful not only in the mapping of the genome, but also for functional studies of proteins of C. gl utamicum. For example, to identify the region of the genome to which a particular DNA binding protein of C. glutamxcum binds, the genome of C. gl utamxcum can be digested, and the fragments incubated with the DNA binding protein. Those that bind with the protein are additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; the union of said nucleic acid molecules with the genome fragment allows the location of the fragment in the genome map of C. glutamx cum and, when it is carried out many times with different enzymes, facilitates a rapid determination of the nucleic acid sequence a which binds the protein. further, the nucleic acid molecules of the present invention can be sufficiently homologous by the sequences of related species such that these molecules can serve as markers for the construction of a geonomic map in related bacteria, such as for example Brevibacterium lactofermentum. The SRT nucleic acid molecules of the invention are also useful for structural and evolutionary protein studies. The resistance processes in which the molecules of the invention participate are used by a wide range of cells; By comparing the sequences of the nucleic acid molecules of the present invention with those encoding similar enzymes of other organisms, the evolutionary relationship of these organisms can be evaluated. Similarly, a comparison of this type allows the evaluation of the regions of the sequence that are conserved and of the regions of the sequence that are not conserved, which can help to determine the regions of the protein that are essential for the functioning of the enzyme. This type of determination is valuable for protein manipulation studies and can provide an indication of what the protein can tolerate in terms of mutagenesis without loss of function. The genes of the present invention, for example, the gene encoding LMRB (SEQ ID NO: 1) or another gene of the invention that encodes a protein for tolerance or resistance to chemical or environmental stress (for example, resistance against one or several antibiotics), can be used as genetic markers for genetic transformation (for example, the transfer of additional genes or the disorganization of pre-existing genes of organisms such as C. gl utamicum or other bacterial species.) The use of these nucleic acid molecules allows efficient selection of organisms that have incorporated a given transgene cassette (eg, a plasmid, phage, phasmid, phagemid, transposon, or other nucleic acid element) based on a trait that allows the survival of the organism in a In another hostile or toxic environment (for example, in the presence of an antimicrobial compound), by using one or more of the genes in the As genetic markers, the speed and ease with which organisms that have desirable transformed traits are manipulated and isolated (for example, modulated production of fine chemicals) are improved. While it is advantageous to use the genes of the present invention for the selection of transformed C. gl utamxcum and related bacteria, it is possible, in accordance with what is described herein, to use homologs (for example, homologs from other organisms), allelic variants or fragments of the gene that preserves the desired activity. In addition, 5 'and 3' regulatory elements ie the genes of the invention can be modified in accordance with what is described herein (for example, by substitution, insertion, removal, replacement of nucleotides with a more desirable genetic element) with the object to modulate the transcription of the gene. For example, a variant of LMR3 in which the nucleotide sequence of the region -1 to -200 5 'up to the initial codon has been altered to modulate (preferably increase) the transcription and / or translation of LMRB can be employed, as well as constructs in which a gene of the invention (e.g., the LMRB gene (SEQ ID NO: 1)) is functionally coupled to one or more regulatory signals (e.g., inducer or repressor binding sequences) that they can be used to modulate gene expression. Similarly, more than a copy of a gene (functional or deactivated) of the invention can be used. An additional application of the genes of the invention (for example, the gene encoding LMRB (SEQ ID NO: 1) or another drug or antibiotic resistance gene) is the discovery of new antibiotics that are active against corynebacteria and / or other bacteria. For example, a gene of the present invention can be expressed (or overexpressed) in a suitable host to generate an organism that has an increased resistance to one or several drugs or antibiotics (in the case of LMRB, lincosamides in particular, especially lincomycin ). This type of resistant host can subsequently be used to screen chemicals with bacteriostatic and / or bacteriocidal activity, such as novel antibiotic compounds. It is possible, in particular, to use the genes of the invention (e.g., the LMRB gene) to identify new antibiotics that are active against these microorganisms that are already resistant to standard antibiotic compounds. The invention offers methods for screening molecules that modulate the activity of a SRT protein, either by interaction with the protein itself or a substrate or a binding partner of the SRT protein, or by modulation of the transcription or translation of the protein. an SRT nucleic acid molecule of the invention. In methods of this type, a microorganism expressing one or more SRT proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the test is evaluated. SRT protein. The manipulation of the SRT nucleic acid molecules of the invention can result in the production of SRT proteins having functional differences from the wild-type SRT proteins. These proteins can be improved in terms of efficiency or activity, they can be present in larger numbers in the cell than in the usual case, or they can have a decreased efficiency or activity. The objective of these manipulations is to increase the viability and activity of the cell when the cell is exposed to stress and the environmental and chemical hazards that often accompany a large-scale fermentative culture. Thus, by increasing the activity or number of copies of a protease regulated by heat shock, the ability of the cell to destroy the incorrectly bent proteins, which may otherwise interfere with the normal functioning of the cell, can be increased. (for example, by continuous binding to substrates and co-factors even when the protein does not have the activity to act on these molecules appropriately). The same is true in the case of overexpression or optimization of activity of one or several chaperone molecules induced by thermal or cold shock. These proteins help the correct dooiado of incipient polypeptide chains, and therefore their increased activity or increased presence should increase the percentage of proteins correctly folded in the cell, which in turn should increase the overall metabolic efficiency and viability of the cells in culture. The overexpression or optimization of the transport molecules activated by osmotic shock would result in an increased capacity on the part of the cell to maintain the intracellular hcmeostasis, thus increasing the viability of these cells in culture. Similarly, ia = ooreproduction or increased activity by mutagenesis of proteins involved in the development of cellular resistance to chemical stresses of various types (either by transporting the offensive chemical out of the cell or by modifying the chemical in a less dangerous substance), it must increase the adaptability of the organism in the environment containing the dangerous substance (ie, large-scale fermentative culture), and therefore may allow the survival of a relatively large number of cells in a culture of this type. The overall effect of all these mutagenesis strategies is to increase the amount of fine chemical producing compounds in the culture, thereby increasing the yield, production, and / or production efficiency of one or more desired fine chemicals from the culture. The list mentioned above of mutagenesis strategies for SRT proteins to result in improved yields of a desired compound is not intended to be a limiting list; Variations of the mutagenesis strategies presented will be readily apparent to a person with ordinary knowledge in the field. Through these mechanisms, the nucleic acid and ein molecules of the present invention can be used to generate C. gl utamx cum or related strains of bacteria expressing nucleic acid molecules and SRT ein with mutations so that ime the yield, uction, and / or uction efficiency of a desired compound. This desired compound can be any natural uct of C. gl utamicum, which includes the final ucts of biosynthetic pathways as well as intermediates of naturally occurring metabolic pathways, as well as molecules that do not occur naturally in the metabolism of C. gl utamicum, but which are uced by a strain of C. gl utamicum of the present invention. This invention is further illustrated by the following examples which should not be construed as limiting the present invention. The contents of all references, patent applications, patents, published patent applications, tables, and the list of sequences mentioned in this application are incorporated by reference. TABLE 1: Genes included in the application Amino Acid- Contig Code. NT NT nucleic acid initial identification end SEQ ID NO SEQ ID NO 1 2 RXA01524 GR00424 29041 30483 3 4 RXA00497 GR00124 52 348 5 6 RXN00493 W0036 14389 16002 7 8 F RXA00498 GP0C124 363 1601 9 10 RXA01217 GR0C3? 3 802 203 11 12 RXA00605 GR00159 7412 5865 13 14 RXA00404 GR00089 2909 594 15 16 RXN03119 W0098 86877 87008 17 18 RXN03120 W0098 87351 87476 19 20 RXN00575 W0323 14716 15252 21 22 F RXA00575 GR00156 2130 1648 Nucleic acid Function. 1 SEQ ID NO I Lincomycin 3 RESISTANCE PROTEIN 3 KEYERONIN 10 KD 5 CHAPERONIN 60 KD 7 CHAPERONIN 60 KD 9 GENERAL STRESS PROTEIN CTC II CATALASE (EC 1.11.1.6) 13 CARBON EXHAUST PROTEIN A 15 SUPERXXIDASE DISMUTASE [MN] ( EC 1.15.1.1) 17 DISMUTASE SUPEROXIDE [MN] (EC 1.15.1.1) 19 PROTEIN OF RESISTANCE TO FOSFINOTRICIN 21 PROTEIN OF RESISTANCE TO PHOSPHINOTRICIN Chaperones Acid Amino- Code of Contig. NT NT nucleic acid initial identification end SEQ ID NO SEQ ID NO 23 24 RXN01345 W0123 4883 3432 25 26 F RXA01345 GR0C391 1172 í 27 28 RXA02541 GR00726 13657 12473 29 30 RXA02542 GR00726 14518 13865 31 32 RXN02543 W0057 22031 20178 33 34 F RXA02543 GR00726 16375 14522 35 36 RXN02280 W0152 1849 26 37 38 F RXA02282 GR00659 1145 1480 39 40 RXA00886 GR00242 12396 13541 41 42 RXS00568 W0251 2928 1582 43 44 RXN03038 W0017 42941 43666 45 46 RXN03039 W0018 2 631 47 48 RXN03040 W0018 761 1069 49 50 RXN03051 W0022 2832 3566 51 52 RXN03054 W0026 1906 3486 53 54 RXN02949 W0025 31243 31575 55 56 RXN02462 W0124 11932 13749 57 58 RXN01559 W0171 7795 5954 59 60 RXN00046 W0119 5363 6058 61 62 RXN01863 W0206 1172 24 63 64 RXN00833 W0180 8039 8533 65 66 RXN01676 W0179 12059 11304 67 68 RXN00380 W0223 836 216 69 70 RXN00937 W0079 42335 42706 71 72 RXN02325 W0047 5527 6393 73 74 RXN01837 W0320 7103"879 75 76 RXN01926 V7C234 i ~ 4i 77 78 RXN02002 W0111 141 518 79 80 RXN02736 W0074 13600 14556 81 82 RXS03217 • 83 84 F RXA01917 GR00549 3465 3665 5 Nucleic acid Func: ion SEQ ID NO 23 Molecular chaperones (HSP70 / Dnak family) 25 Molecular chaperones (family HSP70 / Dnak) 27 PROTEIN: DNAJ 10 29 PROTEIN (GRPE ft 31 PROTEIN: DNAK 33 PROTEIN: DNAK 35 TRAP1 37 Molecular chaperone , family HSP90 15 39 PROTEIN: DNAJ 41 ACTIVATION FACTOR 43 PS1 PROTEIN INITIATOR 45 PROTEIN INITIATOR PSi> 47 PROTEIN INITIATOR PS1 20 49 PROTEIN INITIATOR PS1 51 PROTEIN INITIATOR PS1 53 SECRE SUBUNIDAD DE PROPROTEÍLO TRANSLOCASA 55 SUBUNIDAD SECE DE PREPROTEIN TRANSLOCASÁ 57 PROTEIN: PROTEIN EXPORT MEMBRANE 25 NA SE OJ 59 Signal recognition particle GTPase 61 thioredoxin oxide / O / C 63 TIOL PEROXIDASE (EC 1.11.1.-) 65 TIOL EXCHANGE PROTEIN: DISULFURE DS3D 67 PROTEIN OF TIOL EXCHANGE: DISUL JRO TL? A 69 TIOREDOXIN 71 TIOREDOXIN 73 PEPTIDIL-PROLIL CIS-TRANSISOMERASE (EC 5.2.1. 8) 75 CAD RELEASE FACTOR ENA DE PÉPTIDOS 3 77 PEPTIDE CHAIN RELEASE FACTOR 3 79 OXPPCICLO PROTEIN PUTATIVAS OPCA 81 SMALL CHIQUE PROTEIN FRIED 83 SMALL FRIED SHOCK PROTEIN Proteins involved in stress responses Amino Acid- Contig Code. NT NT nucleic acid initial identification end SEQ ID NO SEQ ID NO 85 86 RXA02184 GR00641 19628 19248 87 88 RXA00810 GR00218 792 992 89 90 RXA01674 GR00467 1878 2771 91 92 RXA02431 GR00708 2 1192 93 94 RXA02446 GR00709 11640 11206 95 96 RXA02861 GR10006 551 1633 97 98 RXA00981 GR00276 3386 4:17 99 100 RXN00786 W0321 1680 706 101 102 RXS01027 W0143 5761 6768 103 104 RXS01528 W0050 17276 16749 105 106 RXS01716 W0319 3259 2774 107 108 RXS01835 W0143 10575 10045 109 110 RXS02497 W0007 15609 16535 111 112 RXS02972 W0319 2763 2353 Nucleic Acid Function SEQ ID NO: 85 COLD SHOCK PROTEIN CSPC 87 SMALL COLD SHOCK PROTEIN 89 PROBABLE ACTIVATOR OF INDUCABLE GENES BY HYDROGEN PEROXIDE 91 Protein inducible by damage P 93 PROTEIN INDUCED C 95 Metallothionein u0308aa-Mycobacterium leprae probable 97 GTP PIROFOSFOQUI ASA (EC 2.7.6.5) 99 PROTEIN LYTB 101 DIADENOSIN 5 ', 5"-Pl, P4-TETRAFOSFATOHIDROLASA (EC.3.6.1.17) 103 DIADENOSIN 5 ', 5" -Pl, P4-TETRAFOSFATOHIDROLASA (EC.3.6.1.17) 105 EXOPOLIFO? FATASA (EC 3.6.1.11) Í07 GUANOSINA-3 , 5 '-BIS (DIFOSPHATE) 3' -PIROFOSFCHI-DROLASE (EC 3.1.7.2) 109 EXOPOLYPHOSPHATASE (EC 3.6.1.11) 111 EXOPOLYPHOSPHATASE (EC 3.6.1.11) Resistance and tolerance Amino acid- Contig code. NT NT nucleic acid initial identification end SEQ ID NO SEQ ID NO 113 114 RXA02159 GR00640 6231 6743 115 116 RXA02201 GROO646 5837 6199 117 118 RXA00599 GR00159 1843 1457 119 120 RXA00600 GR00159 2940 1843 121 122 RXAC2200 GR00646 4651 5760 123 124 RXA02202 GR00646 6278 6916 125 126 RXA02205 GR00646 9871 8993 127 128 RXA00900 GR00245 4052 3201 129 130 RXNC0901 W0140 8581 8168 131 132 F RXA00901 GR00245 4357 3980 133 134 RXA00289 GR00046 3263 4438 135 136 RXN01984 W0056 1515 1811 137 138 'F RXA01984 GR00574 282 4 139 140 RXA00109 GR00015 1176 565 141 142 RXA00109 GR00015 1176 565 143 144 RXA00996 GR00283 1763 1023 145 146 RXN: 0829 w: 18o 7950 5611 147 14d F RXA00629 GPJ 0224 i. Be 149 150 F RXA00834 GR00225 463 2025 151 152 RXA00995 GR00283 1023 283 153 154 RXN00803 W0009 53858 52629 155 156 F RXA00803 GR00214 4560 5162 157 158 RXA01407 GR00410 3918 3028 159 160 RXA01408 GR00410 4384 4184 161 162 RXN01922 W0020 2031 3182 163 164 F RXA01922 GR00552 3 1109 165 166 RXA02060 GROO626 1 339 167 168 RXN01936 W0127 40116 41387 169 170 F RXA01936 GR00555 9796 3975 171 172 F RXA01937 GR00555 '10246 9821 173 174 RXN01010 W0209 3776 4894 175 176 F RXA01010 GR00288 774 177 178 RXN03142 W0136 5754 4612 179 180 F RXA01150 GR00323 3807 2917 181 182 RXN02964 W0102 7931 6714 183 184 F RXA02116 GR00636 911 6 185 186 RXA00858 GR00233 1680 2147 187 188 RXA02305 GR00663 2921 2070 189 190 RXA00084 GR00013 2367 1543 191 192 RXA00843 GR00228 3236 3580 193 194 RXA01052 GR00296 3398 3706 195 196 RXA01053 GR00296 3772 4191 197 198 RXA01054 GR00296 4229 4717 199 200 RXN03123 W0106 808 1245 201 202 F RXA00993 GR00282 641 6 203 204 RXA01051 GR00296 3298 2690 205 206 RXN01873 W0248 2054 819 207 208 F RXA01873 GR00535 855 1946 209 210 RXN00034 W0020 16933 18381 211 212 F RXA02273 GR00655 8058 9002 213 214 RXN03075 W0042 2491 3216 215 216 F RXA02907 GR10044 1395 2120 217 218 RXA00479 GR00119 16290 14101 219 220 RXN03124 W0108 4 963 221 222 F RXA01180 GR00336 4 765 223 224 RXA02586 GR00741 10296 10027 225 226 RXA02587 GR00741 12343 10253 227 228 RXN03042 W0018 2440 1835 229 230 F RXA02893 GR10035 1841 1236 231 232 RXA01616 GR00450 1684 203 233 234 RXA01666 GR00463 2307 3683 235 236 RXA00062 GR00009 13252 11855 237 238 RXA00215 GR00032 13834 15294 239 240 RXN03064 W0038 4892 6223 241 242 F RXA00565 GR00151 4892 5884 243 244 F RXA02878 GR10016 1837 1481 245 246 RXA00648 GROO169 2713 1304 247 248 RXN01320 W0082 13146 11500 249 250 F RXA01314 GR00382 744 4 251 252 F RXA01320 GR00383 1979 1200 253 254 RXN02926 W00S2 11497 9866 255 256 F RXA01319 GR00383 1197 4 257 258 RXA01578 GR00439 1423 29 259 260 RXA02087 GR00629 7076 5730 261 262 RXA02088 GR00629 8294 7080 263 264 RXA00764 GR00204 3284 2169 265 266 RXN03125 W0103 972 1142 267 268 RXN01553 W0135 25201 26520 269 270 RXN00535 W0219 5155 5871 271 272 RXN00453 W0G ~ 6 1173 3521 273 274 RXN00932 W0171 13120 13593 275 276 RXN03022 W0002 65 511 277 278 RXN03151 W0163 489 4 279 280 RXN02832 W0355 547 281 282 RXN00165 W0232 3275 1860 283 284 RXN01190 W0169 8992 10338 285 286 RXN01102 W0059 6128 4884 287 288 RXN00788 W0321 3424 3648 289 290 RXN02119 W0102 11242 9602 291 292 RXN01605 W0I37 7124 5610 293 294 RXN01091 W0326 567 4 295 296 RXS02979 W0149 2150 2383 297 298 RXS02987 W0234 527 294 299 300 RXS03095 W0057 4056 4424 Nucleic Acid Function SEQ ID NO 113 ARGININE HYDROXYMIUM RESISTANCE PROTEIN 115 ARSENATE REDUCTASE 117 ARSENICAL RESISTANCE PROTEIN ACR3 119 ARSENICAL RESISTANCE PROTEIN ACR3 121 ARSENICAL RESISTANCE PROTEIN ACR3 123 PROTEIN ARSENICAL RESISTANCE ACR3 125 BACITRACIN RESISTANCE PROTEIN (UN- DECAPRENOLACHINASE PUTATIVA) (EC 2.7.1.66) 127 BICICLOMYCIN RESISTANCE PROTEIN 129 BICICLOMYCIN RESISTANCE PROTEIN 131 BICICLOMYCIN RESISTANCE PROTEIN 133 CHLORANPHENICOL RESISTANCE PROTEIN 135 PROTEIN OF RESISTANCE TO CLORANPHENICOL 137 PROTEIN OF RESISTANCE TO CHLORANPHENICOL 139 PRECURSOR D? COPPER RESISTANCE PROTEIN C 141 PRECURSOR OF COPPER RESISTANCE PROTEIN C 143 PROTEIN D? UNION WITH ATP OF RESISTANCE TO DAUNORUBICINA DRRA 145 PROTEIN OF RESISTANCE TO DAJJGRJ? ICIJA 147 PROTEIN OF RESISTANCE TO DAUNORUBICINA 149 PROTEIN OF RESISTANCE TO DAUNORUBICINE 151 TRANSMEMBRANE PROTEIN OF RESISTANCE TO DAUNORUBYCIN 153 PROTEIN OF RESISTANCE TO METHYLENOMYCIN A 155 PROTEIN OF RESISTANCE TO METHYLENOMYCIN A 157 PROTEIN OF RESISTANCE TO METHYLENOMYCIN A 159 PROTEIN OF RESISTANCE TO METILENOMYCIN A 161 PROTEIN OF RESISTANCE TO METHYLENOMYCIN A 163 PROTECTION OF RESISTANCE TO METHYLENOMYCIN A 165 MYRA MICINAMYCIN RESISTANCE PROTEIN 167 MACROLIDE EFFLUTE PROTEIN 169 NICKEL RESISTANCE PROTEIN 171 NICKEL RESISTANCE PROTEIN 173 NUQUEL RESISTANCE PROTEIN NORA 175 PROTEIN OF RESISTANCE TO QUINOLONE NORA 177 PROTEIN OF RESISTANCE TO QUINOOLONE NORA 179 PROTEIN OF RESISTANCE TO QUINOLONE NORA 181 PROTEIN OF RESISTANCE TO THE QUINOLONE NORA 183 PROTEIN OF RESISTANCE TO THE QUINOLONE NORA 185 PROTEIN OF RESISTANCE TO TELURIO T? RC 187 DAUNOMYCIN C-14 HYDROXYLASE 189 PROTECTION OF USE OF VIBRIOBACTIN VIUB 191 ARSENATE REDUCTASE 193 MERCURY REDUCTASE (EC 1.16.1.1) 195 MERCURY R? DUCTASE (EC 1.16.1.1) 197 M ? RCURIO REDUCTASE (EC 1.16.1.1) 199 HIGH METAL TOLERANCE PROTEIN PRECURSOR 201 METAL TOLERANCE PROTEIN PRECURSOR HEAVY 203 VANZ PROTEIN, teicoplanin resistance protein 205 Hypothetical drug resistance protein 207 Hypothetical drug resistance protein 209 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 211 Hypothetical drug resistance protein 213 Hypothetical Drug Conveyor 215 Hypothetical Drug Conveyor 217 Hypothetical Drug Conveyor 219 Hypothetical Drug Conveyor 221 Hypothetical Drug Conveyor 223 Hypothetical Drug Conveyor 225 Hypothetical Drug Conveyor 227 Hypothetical Drug Conveyor 229 Hypothetical Drug Conveyor 231 EFFLUX PROTEIN MULTIPLE QACB DRUGS 233 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS 235 MULTIPLE DRUG RESISTANCE PROTECTION B 237 MULTIPLE DRUG RESISTANCE PROTEIN B 239 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 241 MULTIPLE DRUG RESISTANCE PROTECTION B 243 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 245 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 247 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 249 PROT? NA D? R? SYSTEM TO MULTIPLE DRUGS B 251 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 253 PROT? NA D? RESISTANCE TO MULTIPLE DRUGS B 255 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 257 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 259 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 261 PROTEIN OF RESISTANCE TO MULTIPLE DRUGS B 263 PROTEIN BMRU, multiple drug efflux transporter 265 Hypothetical drug transporter 267 Hypothetical permease drug 269 Hypothetical drug resistance protein 271 Hypothetical drug transporter 273 Hypothetical drug transporter 275 MULTIPLE DRUG RESISTANCE PROTEIN B 277 PROTEIN OF RESISTANCE TO THE. MYRA MICINAMYCIN 279 IMMUNITY FACTOR TO LYSOSTAPHIN 281 UNION PROTECTION CCN ATP L? MULTIPLE DRUG RESISTANCE CYCLES 283 PROTECTION OF UNION WITH ATP TYPE OF RESISTANCE TO MULTIPLE DRUGS MDL 285 PROT? NA D? R? SISTENCE TO QUINOLONE NORA 287 PROTEIN OF RESISTANCE TO CHLORANPHENICOL 289 PROTEIN OF UNION WITH ATP OF RESISTANCE TO A201A 290 PROTEIN OF TRANSMEMBRANE OF RESISTANCE TO DAUNORUBICINE 293 PROTEIN MAZG 295 PRECURSOR D? PERIPLASMIC COMPONENT OF TRANSPORTATION PROTEIN OF M? RCURIO 297 COMPONENT PRECURSOR? PROTECTIVE PERIPLASMIC D? TRANSPORT? D? M? RCURIO 299 PROTEIN HOMOLOGOUS ACCESSORY OF CADMIUM EFFLUX SYSTEM TABLE 2 - Excluded Genes Genbank Accession No.: A09073 Gene Name: ppg Gene Function: Fosfoenol pyruvate carboxylase Reference: Bachmann, B. et al. "DNA fragment coding for phosphoenolpiruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and methcd for producing L-amino acids using said strains," Patent:? P 0358940-A 3 21/03/90 Access No. Genbank ™: A45579, A45581, A45583, A45585, A45587 Gene Name: Gene Function: threonine dehydratase Reference: Moeckel, B. et al. "Production of L-isoleucine by means of recombinant micro-organisms with deregulated threonine dehydratase," Patent: WO 9519442-A 5 20/07/95 Access No. to Genbank ™: AB003132 Gene Name: murC; ftsQ; ftsZ Function of the Gene: Reference: Kobayashi, M. et al. "Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria," Biochem. Biophys. Res. Commun., 236 (2): 383-388 (1997) Access No. to Genbank ™: AB0150123 Gene Name: murC; ftsQ Function of the Gene: Reference: Wachi, M. et al. "A murC gene from Coryneform bacteria," Appl. Microbiol. Biotechnol., 51 (2): 223-228 (1999) Access No. to Genbank ™: AB018530 Gen Name: dtsR Gen's Function: Reference: Kimura,? et al. "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium lactofermentum," Biosci. Biotechnci. Biochem., 60 (10): 1565-1570 (199 Genbank Accession No.: AB018531 Gen Name: dtsRl; dtsR2 Gen Generation: Reference: Genbank Accession Number: AB020624 Gene Name: murl Gene Function: D-glutamate racemase Reference: Genbank Accession No.: AB023377 Gene Name: tkt Gene Function: transketolase Reference: Genbank Accession No.: AB024708 Gene Name: gltB; gltD Gene Function : large and small subunits of Glutamine 2-oxoglutarate to inotransferase Reference: Genbank Accession No.: AB025424 Gene Name: acn Gene Function: aconitase Reference: Genbank Accession No.: AB027714 Gene Name: rep Gene Function: Replication Protein Reference : Access No. to Genbank ™: AB027715 Name of Gen: rep; aad Gene Function: Replication protein; aminoglycoside adenyltransferase Reference: Genbank Accession No.: AF005242 Gene Name: argC Gene Function: N-acetylglutamate-5-semialdehyde dehydrogenase Reference: Genbank Accession No.: AF005635 Gene Name: glnA Gene Function: Glutamine Synthetase Reference: Genbank Accession No.: AF030405 Gene Name: hisF Gene Function: Cyclase Reference: Genbank Accession No.: AF030520 Gene Name: argG Gene Function: Argininosuccinate synthetase Reference: No. of Access to Genbank ™: AF031518 Gene Name: argF Gene Function: Ornithine carbam.oiitransferase Reference: Genbank Accession No.: AF036932 Gene Name: aroD Gene Function: 3-dehydroquinate dehydratase Reference: Access No. Genbank ™: AF038548 Gene Name: pyc Gen Function: Pyruvate carboxylase Reference: Genbank Accession No.: AF038651 Gene Name: dciA ?; apt; Reí Function of the Gene: Protein binding with Dipeptide; adenine phosphoribosyltransferase, GTP pyrophosphokinase Reference: Wehmeier, L. et al. "The role of the Corynebacterium glutamicum reí gene in (p) ppGpp metabolism," Microbiology, 144: 1853-1862 (1998) Genbank Accession No.: AF041436 Gene Name: argR Gene Function: Arginine Repressor Reference: Genbank Accession No.: AF045998 Gene Name: impA Gene Function : Inositol monophosphate phosphatase Reference: Genbank Accession No. ': AF04S764 Gene Name: argH Gene Function: Argininosuccinate lyase Reference: Genbank Accession No.: AF049897 Gene Name: argC; argJ; argB; argD; argF; argR; argG; argH Gene Function: N-acetylglutamyl phosphate reductase; ornithine acetyltransferase, N-acetylglutamate kinase; acetyl cortinin transminase; ornithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate lyase Reference: Genbank Accession No.: AFOSO109 Gene Name: inhA Gene Function: Enoil-Acyl Reductase Carrier Protein Reference: Genbank Accession Number ~ v: AF050166 Gene Name: hisG Gene Function: ATP Phosphoribosyltransferase Reference: Accession No. to Genbank ™: AF051846 Gene Name: hisA Gene Function: Fosfopcosylformimino-5-am? No-l-phosphoribosi1-4-imidazolecarboxamide isomerase Reference: Accession No. to Genbar.K: A? 052652 JJ Name of Gene: metA Gene Function: Homoserine O-acetyltransferase Reference: Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacterium glutamicum," Mol.

Cells., 8 (3): 286-29 (1998) Genbank Accession No.: AF053071 Gene Name: aroB Gene Function: Dehydroquinate synthetase Reference: Genbank Accession No.: AF060558 Gene Name: hisH Gene Function: Glutamine Amidotransferase Reference: Genbank Accession No.: AF086704 Gene Name: hisE Gene Function: Phosphoribosyl-ATP-pyrophosphohydrolase Reference: Genbank Accession No.: AF114233 Gene Name: aroA Gene Function : 5-enolpiruvilshiquimato 3-phosphate synthase Reference: Genbank Accession No.: AF116184 Gene Name: panD Gene Function: L-a precursor = parcato-alpha-decareexilaea Reference: Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli, "Appl. Environ Microbiol., 65 (4) 1530-1539 (1999) Genbank Accession No.: AF124518 Gene Name: aroD; aroE Gene Function: 3-dehydrokinase; shiquimate dehydrogenase Reference: No Access to Genbank ™: AF124600 Gene Name: aroC; aroK; aroB; pepQ Gene Function: Corismate synthase; shikimate kinase; 3-dehydroquinate synthase; putative cytoplasmic peptidase Reference: Genbank Accession No.: AF145897 Gen: inhA Gen Function: - Reference: Genbank ™ Accession Number: AF145898 Gen Name: inhA Gen Generation Function: - Reference: Genbank ™ Accession Number: AJ001436 Gene Name: ectP Gene Function: Transport of ectoin, glycine, betaine, proline Reference: Peter, H. et al. "Corynebacterium glutamicum is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline / ectoine uptake system, ProP, and the ectoine / proline / glycine betaine carrier, EctP, "J.

Bacteriol., 180 (22): 6005-6012 (1998) Genbank Accession No.: AJ004934 Gene Name: dapD Gene Function: Tetrahydrodipicolinate succinylase (incomplete1) Reference: Wehrmann, A. et al. "Different modes of diaminopimelate synthesis and their role in cell wall integrity: A study with Corynebacterium glutamicum," J.

Bacteriol., 180 (12): 3159-3165 (1998) Genbank Accession No.: AJ007732 Gene Name: ppc; secG; amt; ocd; soxA Gene Function: Phosphoenolpyruvate carboxylase; ? high affinity ammonium absorption protein; putative ornithine-cyclodecarboxylase; sarcosine oxidase Reference: Genbank Accession No.: AJ010319 Gene Name: ftsY, glnB, glnD, srp; amtP Function of the Gene: Involved in cell division; PII protein; uridyliltransferase (uridylyl removal enzyme); signal recognition particle; low affinity ammonium absorption protein Reference: Jakoby, M. et al. "Nitrogen regulation in Corynebacterium glutamicum; Isolation of genes involved in biochemical characterization of corresponding proteins," FEMS Microbiol., 173 (2): 303-310 (1999) Genbank Accession No.: AJ132968 Gene Name: cat Gene Function: Chloramphenicol Acetyltransferase Reference: Genbank Accession No.: AJ224946 Gene Name: mqo Gene Function: L-malate; quinone oxidoreductase Reference: Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum," Eur. J. Biochem., 254 (2): 395-403 (1998) Genbank Accession No.: AJ238250 Gene Name: ndh Gene Function: NADH dehydrogenase Reference: Genbank Accession No.: AJ238703 Name of Gene: porA Function of Gene: Porin Reference: Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: The channel is formed by a low molecular mass polypeptide, "Biochemistry, 37 (43): 15024-15032 (1998) Access No. to Genbank ™: D17429 Name of the Gene: - Function of the Gene: Element that can be transposed IS31831 Reference: Vertes et al. "Isolation and characterization of IS31831, a transposable element from Corynebacterium glutamicum," Mol. Microbiol., 11 (4): 739-746 (1994) Genbank Accession No.: D84102 Gene Name: odhA Gene Function: 2-oxoglutarate dehydrogenase Reference: Usuda, Y. et al. "Molecular cloning of the Corynebacterium glutamicum (Brevibacterium lactofermentum AJ12036) odhA gene encoding a novel type of 2-oxoglutarate dehydrogenase, "Microbiology, 142: 3347-3354 (1996) Genbank Accession No.: E01358 Gene Name: hdh; hk Gene Function: Ho oserine dehydrogenase; homoserine kinase Reference: Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP 1987232392-A 1 12/10/87 Genbank Accession No. 1: E01359 Gene Name: Function of the Gene: Higher current of the initial codon of the homoserin kinase gene. Reference: Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent JP 1987232392-A 12/10/87 Genbank Accession No.:? 01375 Gene Name: Gene Function: Tryptophan Operon Reference: Access No. Genbank ™:? 01376 Gene Name: trpL; trp? Gene Function: Peptide leader; anthranilate synthase Reference: Matsui, K. et al. "Tryptophan operon, peptide and protein coded that, utilization of tryptophan operen gene expression and production of tryptophan," Patent: JP 1987244382-A 10/24/87 Access No. to Genbank ™:? 01377 Gene Name: Gene Function: Promoter and operator regions of tryptophan operon Reference: Matsui, K. et al. "Tryptophan operon, peptide and protein coded as, utilization of tryptophan operon gene expression and production of tryptophan," Patent: JP 1987244382-A 10/24/87 Genbank Accession No.: 03937 Gene Name: Gene Function: Biotin-synthase Reference: Hatakeyama, K. et al. "DNA fragment containing gene capable of coding biotin synthetase and its utilization, Patent: JP 1992278088-A 02/10/92 Genbank Accession No.: E04040 Gene Name: Gene Function: diamino pelargonic acid aminotransferase Reference: Koha a, K. et al. "" Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utiiization, "Patent: JP 1992330284-A 1 11/18/92 Access No. to Genbank ™ 1: E04041 Name of Gene: Function of Gene: destiobiotinsintasa Reference: Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 Genbank Accession No.:? 04307 Gene Name: Gene Function: Flavum aspartase Reference: Kurusu, Y. et al. "Gene DNA coding aspart and utilization thereof," Patent: JP 1993030977-A 02/09/93 Access No. to Genbank ™:? 04376 Name of Gene: Gene Function: isocitric acid lyase Reference: Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 09/03/93 Access No. to Genbank ™:? 04377 Gene Name: Gene Function: N-terminal fragment of isocitric acid lyase Reference: Katsumata, R . et al. "Gene manifestation controlling DNA," Patent: J? 1993056782-A 3 09/03/93 Access No. to Genbank ™: E04484 Gene Name: Gene Function: Prephenate Hydroxysate Reference: Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 1993076352-A 2 30/03/93 Genba k ™ Accession No.: E05108 Gene Name: Gene Function: Aspartokinase Reference: Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1993184366-A 1 27/07/93 Genbank Accession No.: E05112 Gene Name: Gene Function: Dihydro-dipicorinate synthetase Reference: Hatakeyama, K. et to the. "Gene DNA coding dihydrodipicolinic acid synthetase and its use," Patent: J? 1993184371-A 1 27/07/93 Genbank Accession No.: E05776 Gene Name: Gene Function: diaminopimelic acid dehydrogenase Reference: Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase and its use, "Patent: JP 1993284970-A 1 02/11/93 Access No. to Genbank ™: E05779 Gene Name: Gene Function: Treoninsintasa Reference: Kohama, K. et al. "Gene DNA coding threonine synthase and its use," Patent: JP 1993284972-A 1 02/11/93 Genbank Accession No.: E06110 Gene Name: Gene Function: Prefenate dehydratase Reference: Kikuchi, T. et al, "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A 1 27/12 / 93 Genbank Accession No.: E06111 Gene Name: Gene Function: Prefenate dehydratase with mutation Reference: Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A 1 2"/ 12/93 Access No. to Genbank ™: E06146 Gene Name: Gene Function: acetchidrcxi smtetase acid Reference: Inui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its use, "Patent: JP 1993344893-A 1 27/12/93 Access No. to Genbank ™:? 06825 Gene Name: Gene Function: Aspartoquinasa Reference: Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 08/03/94 Genbank ™ Accession No.:? 06826 Gene Name: Gene Function: Alpha aspartokinase mutant subunit Reference: Sugimoto, M. et al. "Mutant aspartokinase gene," Patent: JP 1994062866-A 1 08/03/94 Genbank Accession No. "M: E06827 Gene Name: Gene Function: Alpha subunit of mutated aspartakinase Reference: Sugimoto, M. et al. "Mutant aspartokinase gene," Patent JP 1994062866-A 1 08/03/94 Genbank Accession No.: E07701 Gene Name: secY Gene Function: Reference: Honno, N. et al. "Gene DNA involved in integration of membrane protein to membrane," Patent: JP 1994169780-A 1 06/21/94 No. ie Access to Genbank ": i: 5177 Gene Name: Gene Function: Aspartokinase Reference: Sato, Y. et a." Genetic DNA capable of coding Aspartokinase released from feedback inhibition and its utilization, "Patent: JP 1994261766-A 1/20/09/94 Genbank Accession No.: E08178, E08179,? 08180,? 08181, 08182 Gene Name: Gene Function: Aspartokinase Release of Feedback Inhibition Reference: Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase reieased from feedback inhibition and its utilization, "Patent: JP 1994261766-A 1/20/09/94 Genbank Accession No.: EC8232 Gene Name: Gene Function: Acetohydroxy acid isomeroreductase Reference: Inui, M. et al. "Gene DNA coding acetohydroxy acid iso eroreductase", Patent: JP 1994277067-A 1 04/10/94 Access No. to Genbank ™:? 08234 Gene Name: sec? Function of the Gene: Reference: Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," Patent: JP 1994277073-A 1 04/10/94 Genbank Accession No. ": E J5643 Gene Name: Gene Function: Promoter Region of FT aminotransferase and destiobiotin synthetase Reference: Hatakeyama, K. et al." DNA fragment having promoter function in coryneform bacterium , "Patent: JP 1995031476-A 1 03/02/95 Access No. to Genbank ™: E08646 Gene Name: Gene Function: Biotinsintetase Reference: Hatakeyama, K. et al. "DNA fragment having promoter function in coryneform bacterium," Patent: JP 1995031476-A 1 03/02/95 Access No. to Genbank7": E08649 Gene Name: Gene Function: Aspartase Reference: Kohama, K. et al." DNA fragment having promoter function in coryneform bacterium, "Patent: JP 1995031478-A 1 03/02/95 Genbank Access Number ::? 08900 Gene Name: Gene Function: Dihydrodipicolinate reductase Reference: Madori, M. et al. "DNA fragment containir. Gene coding Dihydrodipicolinate acid reductase and utilization thereof," Patent: J? 1995C75578-A 1 03/20/95 Genbank Accession No. ": E08901 Gene Name: Gene Function: Diaminopimelic acid decarboxylase Reference: Madori, M. et al. "DNA fragment containing gene coding Diaminopimelic acid decarboxylase and utilization therof," Patent: JP 1995075579-A 1 03/20/95 Genbank Accession No.: E12594 Gene Name: Gene Function: Serine hydroxymethyltransferase Reference: Hatakeyama, K . et al. "Production of L-trypophan," Patent: JP 1997028391-A 1 04/02/97 Genbank Accession No.: E12760, E12759,? 12758 Gene Name: Gene Function: Transposase Reference: Moriya, M. et to the. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 Genbank Accession No. ": E12764 Gene Name: Gene Function: Arginyl-tRNA synthetase; diaminopimelic acid decarboxylase Reference: Moriya, M. et al." Amplification of gene using artificial transposon, "Patent: JP 1997070291-A 03/18/97 Accession No. to Genbank "'': E12767 Name of Gene: Gene Function: Dih dropicolinic acid synthase Reference: Moriya, M. et al." A piíficaticn de gene usir.g artificial transposon, "Patent: JP 1997070291- 3/18/97 Access No. to Genbank ™: E12770 Gene Name: Gene Function: Aspartoquinasa Reference: Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 Genbank Accession No.: E12773 Gene Name: Gene Function: Dihydrodipicolinic acid reductase Reference: Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 Genbank Accession No.: E13655 Gene Name: Gene Function: Glucose-6-phosphate dehydrogenase Reference: Hatakeyama, K. et al. "Glucose-6-phosphate dehydrogenase and DNA capable of coding the same," Patent: JP 1997224661-A 1 02/09/97 Genbank Accession No.: L01508 Gene Name: IlvA Gene Function: Threonine Dehydratase Reference: Moeckel, B. et al. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutamicum," J. Bacteriol., 174: 8065-8072 '1992 * Genbank Accession No.: L07603 Gene Name: EC 4.2.1.15 Gene Function: 3- deoxy-D-arabinoheptulosonate-7-phosphate synthase Reference: Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium glutamicum 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase gene," FEMS Microbiol. Lett., 107: 223-230 (1993) Access No. to Genbank ™: L09232 Gene Name: IlvB; ilvN; ilvC Gene Function: Large subunit of acetohydroxy acid synthase; Small subunit of acetohydroxy acid synthase; isomeroreductase of acetohydroxy acid Reference: Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon," J. Bacteriol., 175 (17): 5595-5603 (1993) Genbank Accession No.: L18874 Gene Name: SRTM Function of the Gene: Phosphoenolpyruvate sugar phosphotransferase Reference: Fouet, A. et al. "Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria," PNAS USA, 84 (24): 8773-8777 (1987); Lee, J.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II and analyzes of the deduced protein sequence," FEMS Micrebiel. Lett., 119 (1-2): 137-145 (1994) Genbank Accession No.: L27123 Gene Name: aceB Gene Function: Malate synthase Reference: Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J. Microbiol. Biotechnol., (4): 256-263 (1994) Genbank Accession No.: L27126 Gene Name: - Gene Function: Pyruvate kinase Reference: Jetten, M.S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," Appl. Environ. Microbiol., 60 (7): 2501-2507 (1994) Genbank Accession No.: L28760 Gene Name: aceA Gene Function: Isocitrate lyase Reference: Genbank Accession No.: L35906 Gene Name: dtxr Function of the Gene: Diphtheria toxin repressor Reference: Oguiza, JA et al. "Molecular cloning DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium lactofermentum, "J. Bacteriol., 177 (2): 465-467 (1995) Nc. Access to Genbank": M13774 No ore dei Gen: - Function of Gene: prefenate dehydratase Reference: Follettie, M.T. et al. "Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene," J. Bacteriol., 167: 695-702 (1986) 5 Genbank Accession No.: M16175 Gene Name: 5S rRNA Gene Function: - Reference: Park, YH et al. "Phylogenetic analysis of the bacterium coryneform by 56 rRNA sequences," J. Bacteriol., 10 169: 1801-1806 (1987) Genbank Accession No. 1 '' ": M16663 Gene Name: trpE Gene Function: Anthranilate synthase, 5 'end Reference: Sano, K. et al. "Structure and function of the 15 trp operon control regions of Brevibacterium lactofermentum, to glutamic-acid-producing bacterium," Gene, 52: 191-200 (1987) Accession No. to Genbank ™: M16664 f¡f¡ Gene Name: trpA Gene Function: Tryptophan synthase, 3 'end 20 Reference: Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium," Gene, 52: 191-200 (1987) Accession No. to Genbank ™: M25819 Gene Name: 25 Gene Function: Phosphoenoipyruvate carbcxiiase Reference: O'Regan, M. et al. "Cloning and Nucleotide Sequence of the Phosphoenolpyruvate carboxylase-coding of Corynebacterium glutamicum ATCC 13032," Gene, 77 (2) 237-251 (1989) Genbank Accession No.: M85106 Gene Name: Gene Function: Sequence of insertion of 23S rRNA gene Reference: Roller, C. et al. "Gram-positive bacteria with a high DNA G + C content are charecterized by a common insertion within their 23S rRNA genes," J. Gen. Microbiol., 138: 1167-1175 (1992) Access No. to Genbank ™: M85107 , M851C5 Gene Name: Gene Function: 23S rRNA insertion sequence Reference: Roller, C. et al. "Gram-positive bacteria with a high D? A G + C content are characterized by a copy insertion within their 23S rR? A genes," J. Gen. Microbiol., 138: 1167-1175 (1992)? O. Access to Genbank ™: M89931 Gene Name: aecD; brnQ; yhbw Gene Function: Beta C-S lyase; branched-chain amino acid absorption vehicle; hypothetical protein and tw Reference: Rossol, I. et al. "The Corynebacterium slutamicum aecD gene encodes to C-S lyase with alpha, beta-elimination activity that degraaes aminoethyicysteme," J. Bacterio!., 174 (9): 2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the gene product," Arch. Mirobiol., 169 (4): 303-312 (1998) Genbank Accession No.: S59299 Gene Name: trp Gene Function : Lead Generator (promoter) Reference: Herry, DM et al. "Cloning of the trp gene cluster from a tryptophan-hyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence," Appl. ? nviron. Microbiol., 59 (3): 791-799 (1993) Genbank Accession No. 1: U11545 Gene Name: trpD Gene Function: Phosphoribosyltransferase anthranilate Reference: O'Gara, J.P. and Dunican, L.K. (1994) Complete nucleotide sequence of the Corynebacterium glutamicum ATCC 21850 tpD gene. "Thesis, Microbiology Department, University College Galway, Ireland Genbank Accession No.: U13922 Gene Name: cglIM; cglIR; clglIR Gene Function: 5 - putative type II methylcyanosis methyltransferase; putative type II restriction endonuclease; type I or putative type II restriction endonuclease Reference: Schafer, A. et al. "Cloning and characterization of a DNA region encoding a stress - sensitive restrictior. system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli, "J. Bacteriol., 176 (23): 7309-7319 (1994); Schafer, A. et al. "The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine in an McrBC-deficient? Scherichia coli strain," Gene, 203 (2): 95-101 (1997) Genbank Accession No.: U14965 Gene Name: recA Function of Gen: Reference: Genbank Accession No.: U31224 Gene Name: ppx Gene Function: - Reference: Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J. Bacteriol, 178 (15): 4412-4419 (1996) Genbank Accession No.: U31225 Gene Name: proC Gene Function : L-proline; NADP + 5-oxidoreductase Reference: Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the prcA step, "J. Bacteriol., 178 (15): 4412-4419 (1996) Access No. to Genbank: U3123: Gene Name: obg; proB, unkdh Function of the Gen:?; gamma glutamyl kinase; similar to D-hydroxy acid dehydrogenase specific for D isomers Reference: Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J. Bacteriol., 178 (15): 4412-4419 (1996) Genbank Accession No.: U31281 Gene Name: bioB Gene Function: Biotin synthase Reference: Serebriiskii, IG, "Two new members of the bio B superfamily: Cloning, sequencing and expression cf the B genes of Methylobacilius flagellatum and Corynebacterium glutamicum, "Gene, 175: 15-22 (1996) Genba .i ™ Accession No.: U35023 Gene Name: thtR; accBC Gene Function: Thiosulfate sulfurtransferase; Acyl CoA carboxylase Reference: Jager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylase and biotin-carboxyl-carrier proteins," Arch.

Microbiol., 166 (2); 76-62 (1996) Genbar.k ™ Accession No.: U43535 Gene Name: cmr Gene Function: Multiple Drug Resistance Protein Reference: Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli, "J. Bacteriol., 179 (7): 2449-2451 (1997) Genbank Accession No.: U43536 Gene Name: clpB Gene Function: Heat shock ATP binding protein Reference: Genbank Accession No.: U53787 Gene Name: aphA-3 Gene Function: 3 '5"-aminoglycoside phosphotransferase Reference: Genbank Accession No.: U89648 Gene Name: -Gen Gene Function: Sequence unidentified Corynebacterium glutamicum involved in histidine biosynthesis, partial sequence Reference: Genbank Accession No.: X04960 Gene Name: trpA; trpB; trpC, trpD; trpE, trpG; trpl Gene Function: Tryptophan Operon Reference: Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of the Brevibacterium lactofermentum tryptophan operon," Nucleic Acids? Es., 14 (24): 10113-1G114 (1966) Genbank Accession No.: X07563 Gene Name: lys A Function of the Gene: DAP decarboxylase (meso-diaminopimelate decarboxylase, EC 4.1.1.20) Reference: Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium glutamicum and possible mechanisms for modulation of its expression," Mol. Gen. Genet., 212 (1): 112-119 (1988) Genbank Accession No.: X14234 Gene Name: EC 4.1.1.31 Gene Function: Phosphoenolpyruvate carboxylase Reference: Eikmanns, B.J. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequences, and expression," Mol. Gen. Genet., 218 (2): 330-339 (1989); Lepiniec, L. et al. "Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution," Plant. Mol. Biól. , 21 (3): 487-502 (1993) Genbank Accession No.: X17313 Gene Name: fda Gene Function: Fructose-bisphosphate aldolase Reference: Von der Osten, C.H. et al. "Molecular cioning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1, 6-biphosphate aldolase to class I and class II aldolases," Mol. Microbiol., Accession No. to Genbank ™: X53993 Gene Name: dapA Gene Function: L-2, 3-dihydrodipicolinate synthetase (EC 4.2.1.52) Reference: Bonnassie, S. et al. "Nucleic sequence of the dapA gene from Corynebacterium glutamicum," Nucleic Acids Res., 18 (21): 6421 (1990) Access No. to Genbank ™: X54223 Gene Name: - Gene Function: Site related to AttB Ref rrence: Cianciotto; N. et al. "DNA sequence homology between att B-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of lambdacorynephage, "FEMS, Microbiol, Lett., 66: 299-302 (1990) Genbank Accession No.: X54740 Gene Name: argS; lysA Gene Function: Arginyl-tRNA synthetase; Diaminopimelate decarboxylase Reference: Marcel, T. et al. "Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene," Mol. Microbiol., 11): 1819-1830 (1990) Access No. to Genbank ™: X55994 Gene Name: trpL; trpE Gene Function: Putative leader peptide; component 1 of anthranilate synthase Reference: Heery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamicum trp? gene, "Nucleic Acids Res., 18 (23): 7138 (1990) Genbank Accession No.: X56037 Gene Name: thrC Gene Function: Threonine synthase Reference: Han, KS et al." The molecular structure of the Corynebacterium glutamicum threonine synthase gene, "Mol.

Microbiol., 4 (10): 1693-1702 (1990) Genbank Accession No. "": X56075 Gene Name: attB-related site Gen Function: Fixation Site Reference: Cianciotto, N. et al. "DNA sequence homology between att B-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of lambdacorynephage, "FEMS, Microbiol, Lett., 66: 299-302 (1990) Genbank Accession No.: X57226 Gene Name: lysC-alpha; lysC-beta; asd Gen Function: Aspartokinase alpha subunit; Beta subunit of aspartokinase; aspartate beta semialdehyde dehydrogenase Reference: Kalinowski, J. et al. "Genetic ana biochemical analysis of the Aspartokinase from Corynebacterium glutamicum," Mol. Microbiol., 5 (5): 1197-1204 (1991); Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspartate beta-semialdehyde dehydrogenase gene asd in Corynebacterium glutamicum," Mol. Gen. Genet., 224 (3): 317-324 (1990) Access No. to Genbank ™: X59403 Name of the Gene: gap; pgk; tpi Gene Function: Glyceraldehyde-3-phosphate; phosphoglycerate kinase; triosephosphate isomerase Reference: Eikmanns, B.J. "Identification, sequence analysis, and expression of a Corynebacterium glutamicu gene cluster encoding the three glycolytic enzymes glyceraldehyde- 3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomers," J. Bacteriol., 174 (19): 6076-6086 ( 1992) Genbank Accession No.: X59404 Gene Name: gdh Gene Function: Glutamate Dehydrogenase Reference: Bormann, ER et al. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase, "Mol.Microbiol., 6 (3): 317-326 (1992) Genbank Accession No.: X60312 Gene Name: lysl Function to Gene: L-lysine permease Reference: Seep -Feldhaus, AH et al. "Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake," Mol. Microbiol., 5 (12): 2995-3005 (1991) Genbank Accession No.: X66078 Gene Name : copl Function of the Gene: Psl protein Reference: Joliff, G. et al. "Cloning and nucleotide sequence of the cspl gene encoding PS1, one of the two major secreted proteins of Corynebacterium glutamicum: The deduced N-terminal region of PS1 is similar to the Mycobacterium antigen 85 complex, Mol. Microbiol., 6 (16): 2349-2362 (1992) Access No. to Genbar. ™: X66112 Gene Name: glt Gene Function: Citrate synthase Reference: Eikmanns, B.J. et al. "Cloning sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase, "Microbiol., 140: 1817-1828 (1994) Genbank Accession No.: X67737 Gene Name: dapB Gene Function: Dihydrodipicolinate reductase Reference: Access No. to Genbank ™: X69103 Gene Name: csp2 Gene Function: PS2 surface layer prickle Reference: Peyret, JL et al. "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacterium glutamicum," Mol. Microbiol., 9 ( 1): 97-109 (1993) Genbank Accession No.: X69104 Gene Name: - Gene Function: Insertion element related to IS3 Reference: Bona and, C. et al. "Identification of IS1206, to Corynebacterium glutamicum IS3-related insertion sequence and phylogenetic analysis, "Mol.Microbiol., 14 (3): 571-581 (1994) Genbank Accession No.: X70959 Gene Name: leuA Gene Function: Isopropylpylate Synthase Reference: Patek , M. et al. "Leucine synthesis in Corynebacterium glutamicum: enzyme activity ties, structure of leuA, and effect of leuA inactivation on lysine synthesis, "Appl. Environ. Microbiol., 60 (1): 133-140 (1994) Genbank Accession No.: X71489 Gene Name: icd Gene Function: Isocitrate dehydrogenase (NADP +) Reference: Eikmanns, B.J. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J. Bacteriol., 177 (3): 774-782 (1995) Access No. to Genbank ™: X72855 Gene Name: GDSRT Gene Function: Glutamate dehydrogenase (NADP +) Reference: Genbank Accession No.: X75083, X70584 Gene Name: mtrA Gene Function: Resistance to 5-methyltryptophan Reference: Heery, DM et al. "A sequence from a tryptophan-hyperproducing strain of Corynebacterium glutamicum encoding resistance to 5-methyltryptophan," Biochem. Biophys. Beef.

Commun., 201 (3): 1255-1262 (1994) Genbank Accession No.: X75085 Gene Name: recA Gene Function: -Reference: Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutamicum and Brevibacterium lactofermentum," Appl. Microbiol. Biotechnol., 42 (4): 575-580 (1994) Access No. to Genbank ™: X75504 Gene Name: aceA; thiX Function of the Gene: Partial isocytilate lyase; ? Reference: Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of enzyme," J. Bacteriol., 176 (12): 3474-3463 994) Genbank Accession No.: X76875 Gene Name: - Gene Function: Beta subunit of ATPase Reference: Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes," Antonie Van Leeuwenhoek, 64: 285-305 (1993) Genbank Accession No.: X77034 Gene Name: tuf Gene Function: Elongation Factor Your Reference: Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factur Tu and ATP-synthase beta-subunit genes," Antonie Van Leeuwenhoek, 64: 285-305 (1993) Genbank Accession No.: X77384 Gene Name: recA Gene Function: - Reference: Billman-Jacobe, H. "Nucleotide sequence of a recA gene from Corynebacterium glutamicum," DNA Seq., 4 (6): 403-404 (1994) Genbank Accession No.: X78491 Gene Name: aceB Gene Function: Malate synthase Reference: Reinscheid, DJ et al. "Malate synthase from Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase: sequence analysis," Microbiology, 140: 3099-3108 (1994) Genbank Accession No.: X80629 Gene Name: 16S rDNA Gene Function: 16S ribosomal RNA Reference : Rainey, FA et al. "Phylogenetic analysis of the genera Rhodococcus and? Orcardia and evidence for the evolutionary origin of the genus? Orcadia from within the radiation of Rhodococcus species," Microbiol., 141: 523-528 (1995)? O. Access to Genbank ™: X81191? Gen Name: gluA; gluB; gluC; gluD Function of the Gene: Glutamate absorption system Reference: Kronemeyer, W. et al. "Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum," J. Bacteriol., 177 (5): 1152-1158 (1995)? O. Access to Genbank ™: X81379 Gene Name: dapE Gene Function: Succinyldiaminopimelate Deuccinylase Reference: Wehrmann, A. et al. "Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of? Scherichia coli," Microbiology, 40: 3349-56 (1994) Genbank Accession No.: X82061 Gene Name: 16S rDNA Gene Function: 16S Ribosomal RNA Reference : Ruimy, R. et al. "Phylogeny of the genus Corynebacterium deduced from Analyzes of small-subunit ribosomal D? A sequences, "Int. J. Syst. Bacteriol. 45 (4): 740-746 (1995)? Or Genbank Access: X82928 Gene Name: asd; lysC Gene Function: Aspartate-semialdehyde dehydrogenase,? Reference: Serebrijski, I. et al. "Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants," J. Bacteriol., 177 (24): 7255-7260 (1995) Genbank Accession No.: X82929 Gene Name: proA Gene Function: Gamma-glutamylphosphate reductase Reference: Serebrijski, I. et al. "Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants," J. Bacteriol., 177 (24): 7255-7260 (1995) Genbank Accession No. ': X84257 Gene Name: 16S rDNA Gene Function: 16S ribosomal RNA Reference: Pascual, C. et al. "Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences," Int. J. Syst. Bacteriol., 45 (4): 724-726 (1995) Access No. to Genbank ™: X85965 Name of Gene: aroP; dapE Function of the Gene: aromatic amino acid permease; ? Reference: Wehrmann et al. "Functional analysis of sequences adjacent to dapE of C. glutamicum proline reveáis the presence of aroP, which encodes the aromatic amino acid transporter," J. Bacteriol., 177 (20): 5991-5993 (1995) Genbank Accession No. ™: X86157 Gene Name: argB; argC; argD; argF; argj Gen Function: Acetylglutamate kinase, N-acetyl-gamma-glutamyl phosphate reductase; acetylornithine aminotransferase; ornithine carbamoyltransferase; glutamate N-acetyltransferase Reference: Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway," Microbiology, 142: 99-108 (1996) Genbank Accession No.: X89084 Gene Name: pta; ackA Gene Function: Phosphate Acetyltransferase; acetate kinase Reference: Reinscheid, D.J. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase," Microbiology, 145: 503-513 (1999) Genbank Accession No.: X89850 Gene Name: attB Function of the Gen: Fixation site Reference: Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J. Bacteriol., 178 (7): 1996-2004 (1996) Genbank Accession No.: X90356 Gene Name: Gene Function: F promoter fragment Reference: Patek, M. et to the. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No. "J X90357 Gene Name: Gene Function: F2 Promoter Fragment Reference : Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No.: X90358 Gene Name: Function of the Gene: FIO promoter fragment Reference: Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No. ™ ': X90359 Name of the Gene: Gene Function: F13 promoter fragment Reference: Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No.: X90360 Gene Name: - Gene Function: F22 Promoter Fragment Reference: Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No.: X90361 Gene Name: Gene Function: F34 Promoter Fragment Reference : Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No.: X90362 Gene Name: Gene Function: F37 Promoter Fragment Reference : Patek, M. et al. "Promoters from C. glutamicum: cloning molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) Genbank Accession No.: X90363 Gene Name: Gene Function: F45 Promoter Fragment Reference: Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X90364 Gene Name: Gene Function: F64 Promoter Fragment Reference: Patek , M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X90365 Gene Name: Gene Function: F75 Promoter Fragment Reference: Patek , M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X90366 Gene Name: Gene Function: FP101 Promoter Fragment Reference: Patek , M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X90367 Gene Name: Gene Function: FP104 Promoter Fragment Reference: Patek , M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X90368 Gene Name: Gene Function: FR109 Promoter Fragment Reference: Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142.1297-1309 (1996) Genbank Accession No.: X93513 Gene Name: at Gene Function: Ammonia Transport System Reference : Siewe, RM et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier of Corynebacterium glutamicum," J. Biol. Chem., 271 (10): 5398-5403 (1996) Genbank Accession No.: X93514 Gene Name: betP Gene Function: Glycine betaine transport system Reference: Peter, H. et al. "Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the compatible solute glycine betaine," J. Bacteriol., 178 (17): 5229-5234 (1996) Access No. to Genbank ™: X95649 Gene Name: orf4 Gen Function: Reference: Patek, M. et al. "Identification and transcriptional analysis of the dapB-ORF2-dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine sinthesis, "Biotechnol.Lett., 19: 1113-1117 (1997) Genbank Accession No.: X96471 Gene Name: lys ?; lysG Gene Function: Lysine exporting protein; lysine Reference: Vrljic, M. et al. "A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum, "Mol. Microbiol., 22 (5): 815- 826 (1996) Genbank Accession No.: X96580 Gene Name: panB; panC; xylB Gene Function: 3-methyl-2-oxobutanoate hydroxymethyltransferase; pantoate-beta-alanine lyase; xylulokinase Reference: Sahm, H. et al. "D-pantothenate synthesis in Corynebacterium glutamicum and use of panBC and genes encoding L-valine synthesis for D-pantothenate overproduction, "Appl.? Nviron. Microbiol., 65 (5): 1973- 1979 (1999) Genbank Accession No.: X96962 Name of the Gene: -Gen Function: Insertion sequence IS1207 and transpo = handle Reference: Genbank Accession No.: X99289 Gene Name: - Gene Function: Elongation Factor P Reference: Ramos, A. et al. "Cloning , sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermentum (Corynebacterium glutamicum ATCC 13869), "Gene, 198: 217-222 (1997) Genbank Accession No.: Y00140 Gene Name: thrB Gene Function: Homoserin kinase Reference: Mateos, LM et al." Nucleotide sequence of the homoserine kinase (thrB) gene of the Brevibacterium lactofermentum, "Nucleic Acids Res., 15 (9): 3922 (1987) Genbank Accession No.: Y00151 Gene Name: ddh Gene Function: Meso-diaminopimelate D- dehydrogenase (? C 1. 4.1.16) Reference: Ishino, S. et al. "Nucleotide sequence of the meso-diaminopimelate D-dehydrogenase gene from Corynebacterium giutamicum, "Nucleic Acids Res., 15 (9): 3917 (1987) Genbank Accession No.: Y00476 Gene Name: thrA Gene Function: Homoserine dehydrogenase Reference: Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacterium lactofermentum," Nucleic Acids Res., 15 (24): 10598 (1987) Genbank Accession No.: Y00546 Gene Name: hom; thrB Gene Function: Homoserine dehydrogenase; homoserin kinase Reference: Peoples, O.P. et al. "Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon," Mol. Microbiol., 2 (1): 63-72 (1988) Genbank Accession No. ': Y08964 Gene Name: murC; ftsQ / divD; ftsZ Gene Function: UDP-N-acetylmuramate-alanine ligase; split-start protein or cell-division protein; cell division protein Reference: Honrubia, M.P. et al. "Identification, characterization, and chromosomal organization of the ftsZ gene from Brevibacterium lactofermentum," Mol. Gen. Genet. 259 (1): 97-107 (1998) Genbank Accession No.: Y09163 Gene Name: putP Gene Function: High affinity proline transport system Reference: Peter, H. et al. "Isolation of the putP gene the Corynebacterium glutamicumproline and characterization of a low-affinity uptake system for compatible solutes," Arch.

Microbiol., 168 (2): 143-151 (1997) Genbank Accession No.: Y09548 Gene Name: pyc Gene Function: Pyruvate carboxylase Reference: Peters-Wendisch, P.G. et al. "Piruvate carboxilase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene," Microbiology, 144: 915-927 (1998) Genbank Accession No.: Y09578 Gene Name: leuB Gene Function: 3-? Sopropilmalate dehydrogenase Reference: Patek, M. et al. "Analysis of the leuB gene from Corynebacterium glutamicum," Appl. Microbiol. Biotechnol., 50 (1): 42-47 (1998) Genbank Accession No.: Y12472 Gene Name: Gene Function: Phi-16 bacteriophage binding site Reference: Moreau, S. et al. "Site-specific integration of corynebacterium Phi-16: The construction of an integration vector," Microbiol., 145: 539-548 (1999) Genbank Accession No.: Y12537 Gene Name: proP Gene Function: Protein of proline / ectoin absorption system Reference: Peter, H. et al. "Corynebacterium glutamicum is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline / ectoine uptake system, ProP, and the ectoine / proline / glycine betaine carrier, EctP, "J.

Bacteriol., 180 (22): 6005-6012 (1998) Genbank Accession No.: Y13221 Gene Name: glnA Gene Function: Glutamine synthetase I Reference: Jakoby, M. et al. "Isolation of Corynebacterium glutamicum glnA gene encoding glutamine synthetase I," FEMS Microbiol. Lett., 154 (1): 81-88 (1997) Genbank Accession No.: Y16642 Gene Name: Ipd Gene Function: Dihydrolipoamide dehydrogenase Reference: Genbank Accession No.: Y18059 Gene Name: - Function of the Gene: Fixation site of Corinéfago 304L Reference: Moreau, S. et al. "Analysis of the integration functions of φ 304L: An integrase module among corynephages," Virology, 255 (1): 150-159 (1999) Genbank Accession No.: Z21501 Gene Name: argS; lysA Function of the Gene: Arginyl-tRNA synthetase, decarboxylase diaminopimelate (partial) Reference: Oguiza, J.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: Regulation of argS-lysA cluster expression by arginine," J. Bacteriol., 175 (22): 7356-7362 (1993) No Access to Genbank ™: Z12502 Gene Name: dapA; dapB Gene Function: Dihydrodipicolinate synthase; Dihydrodipicolinate reductase Reference: Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function, "J. Bacteriol., 175 (9): 2743- 2749 (1993) Access No. to Genbank ™: Z29563 Name of Gene: thrC Gene Function: Threonine synthase Reference: Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded threonine synthase," Appl.

Environ. Microbiol. 60 (7) 2209-2219 (1994) Genbank Accession No.: Z46753 Gene Name: 16S rDNA Gene Function: Gene for 16S Ribosomal RNA Reference: Genbank Accession No.: Z49822 Gene Name: NEXT Function of the Gene: Sigma Factor SigA Reference: Orguiza, JA et al. "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J. Bacteriol., 178 (2): 550-553 (1996) Genbank Accession No.: Z49823 Gene Name: galE; dtxR Gene Function: Catalytic activity of UDP-galactose-4-epimerase; diphtheria toxin regulatory protein Reference: Orguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of Brevibacterium lactofermentum is coupled transcriptionally to the dmdR gene, "Gene, 177: 103-107 (1996) Genbank Accession No.: Z49824 Gene Name: orfl, sigB Gene Function:?; sigma factor SigB Reference: Orguiza, JA et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and SigB," J. Bacteriol., 178 (2): 550-553 (1996) Genbank Accession No.: Z66534 Gene Name: - Gene Function: Transposase Reference: Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacterium lactofermentum ATCC 13869," Gene, 170 (1): 91- 94 (1996) 1 A sequence for this gene was published in the aforementioned reference, however, the sequence obtained by the inventors of the present application is significantly longer than the published version.It is believed that the published version was based on a incorrect start codon, and therefore represents only a fragment of the actual coding region. 10 Table 3: Strains of Corynebacterium and Brevibacterium that can • used in the practice of the invention Genus Species ATCC FERM NRRL CECT Brevibacterium ammoniagenes am oniagenes 21054 19350 Brevibacterium ammoniagenes 15 Brevibacterium ammoniagenes 19351 19352 Brevibacterium ammoniagenes Brevibacterium ammoniagenes .Brevibacterium 19353 19354 19355 Brevibacterium ammoniagenes 20 Brevibacterium ammoniagenes Brevibacterium ammoniagenes 19356 21055 21077 Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes 21553 21580 39101 Brevibacterium ammoniagenes 25 Brevibacterium Brevibacterium divaricatum butanicum 21196 21792 P928 Brevibacterium flavum 21474 Brevibacterium flavum 21129 Brevibacterium flavum 21518 Brevibacterium flavum B11474 Brevibacterium flavum B11472 Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium flavum 21517 Brevibacterium flavum 21528 Brevibacterium flavum 21529 Brevibacterium flavum B11477 Brevibacterium flavum B11478 Brevibacterium flavum 21127 Brevibacterium flavum B11474 Brevibacterium healii 15527 Brevibacterium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacterium lactofermentum 70 Brevibacterium lactofermentum 74 Brevibacterium lactofermentum Brevibacterium lactofermentum 77 Brevibacterium lactofermentum 21798 Brevibacterium lactofermentum 21799 Brevibacterium lactofermentum 21800 Brevibacterium lactofermentum 21801 Brevibacterium lactofermentum B11470 Brevibacterium lactofermentum B11471 Brevibacterium lactofermentum 21086 Brevibacterium lactofermentum 21420 Brevibacterium lactofermentum 21086 Brevibacterium linens 31269 Brevibacterium linens 9174 Brevibacterium linens 19391 Brevibacterium paraffinolyticum Brevibacterium spec 8377. Brevibacterium spec. Brevibacterium spec. 14604 Brevibacterium spec. 21860 Brevibacterium spec. 21864 Brevibacterium spec. 21865 Brevibacterium spec. 21866 Brevibacterium spec. 19240 Corynebacterium acetoacidophilum 21476 Corynebacterium acetoacidophilum 13870 Corynebacterium acetoglutamicum B11473 Corynebacterium acetoglutamicum B11475 Corynebacterium acetoglutamicum 15806 Corynebacterium acetoglutamicum 21491 Corynebacterium acetoglutamicum 31270 Corynebacterium acetophilum B3671 Corynebacterium ammoniagenes 6872 Corynebacterium ammoniagenes 15511 Corynebacterium fujioke 21496 Corynebacterium glutamicum 39137 Corynebacterium glutamicum 21254 Corynebacterium glutamicum 21255 Corynebacterium glutamicum 31830 Corynebacterium glutamicum 13032 Corynebacterium glutamicum 14305 Corynebacterium glutamicum 15455 Corynebacterium glutamicum 13058 Corynebacterium glutamicum 13059 Corynebacterium glutamicum 13060 Corynebacterium glutamicum 21492 Corynebacterium glutamicum 21513 Corynebacterium glutamicum 21526 Corynebacterium glutamicum 21543 Corynebacterium glutamicum 13287 Corynebacterium glutamicum 21851 Corynebacterium glutamicum 21253 Corynebacterium glutamicum 21514 Corynebacterium glutamicum 21516 Corynebacterium glutamicum 21299 Corynebacterium glutamicum 21300 Corynebacterium glutamicum 39684 Corynebacterium glutamicum 21488 Corynebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutamicum 21158 Corynebacterium glutamicum 21159 Corynebacterium glutamicum 21355 Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutamicum 21565 Corynebacterium glutamicum 21566 Corynebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacterium glutamicum 21572 Corynebacterium glutamicum 21573 Corynebacterium glutamicum 21579 Corynebacterium glutamicum 19049 Corynebacterium glutamicum 19050 Corynebacterium glutamicum 19051 Corynebacterium glutamicum 19052 Corynebacterium glutamicum 19053 Corynebacterium glutamicum 19054 Corynebacterium glutamicum 19055 Corynebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium glutamicum 19058 Corynebacterium glutamicum 19059 Corynebacterium glutamicum 19060 Corynebacterium glutamicum 19185 Corynebacterium glutamicum 13286 Corynebacterium glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium glutamicum 21544 Corynebacterium glutamicum 21492 Corynebacterium glutamicum B8183 Corynebacterium glutamicum B8182 Corynebacterium glutamicum B1241-.

Corynebacterium glutamicum B12417 Corynebacterium glutamicum B12418 Corynebacterium glutamicum B11476 Corynebacterium glutamicum 21608 Corynebacterium lilium P973 Corynebacterium nitrilophilus 21419 Corynebacterium spec. P4445 Corynebacterium spec. P4446 Corynebacterium spec. 31088 Corynebacterium spec. 31089 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 Corynebacterium spec. 21857 Corynebacterium spec. 21862 Corynebacterium spec. 21863 Genus Species NCIMB CBS Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium ammoniagenes Brevibacterium butanicum Brevibacterium divaricatum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium flavum Brevibacterium f1avum Brevibacterium. Brevibacterium flavum Brevibacterium ketoglutamicum Brevibacterium healii ketoglutamicum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium ketosoreductum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium lactofermentum Brevibacterium linens Brevibacterium paraffinolyticum Brevibacterium linens 11160 Brevibacterium linens Brevibacterium spec. 717.73 Brevibacterium spec. ^ 17.73 Brevibacterium spec. Brevibacterium spec. Brevibactepum spec.

Brevibacterium spec. Brevibacterium spec. Brevibacterium spec. Corynebacterium acetoacidophilum Corynebacterium acetoacidophilum Corynebacterium acetoglutamicum Corynebacterium acetoglutamicum Corynebacterium acetoglutamicum Corynebacterium acetoglutamicum Corynebacterium acetoglutamicum Corynebacterium acetophilum Corynebacterium ammoniagenes 2399 Corynebacterium ammoniagenes Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium fujioke Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium Corynebacterium Liliu nitrilophilus 11594 Corynebacterium spec. Corynebacterium spec. Corynebacterium spec. Corynebacterium spec. Corynebacterium spec. Corynebacterium spec. Co'rynebacterium spec. Corynebacterium spec. 20145 Corynebacterium spec. Corynebacterium spec. Corynebacterium spec. A "C: American Type Culture Collection, Rcc / iville, Xi, United States of America F? RM: Fermentation Research Institute, Chiba Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, United States C? CT: Spanish Type Culture Collection, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, United Kingdom CBS: Centraalbureau voor Schi elcultures, Baarn, NL NCTC: National Collection of Type Cultures, London, United Kingdom DSMZ: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany For reference see Sugawara, H. et al. (1993) World directory of collections of microorganisms cultures: Bacteria, fungi and yeasts (4th Edition) World federation for culture collections world data center on microorganisms, Saimata, Japan Table 4: Alignment Results ID # Length Results Source Length Genbak Access (NT) rxa00062 1521 GB_HTG2: AC007366 185001 AC007366 rxa00084 948 G3_PR3: HSU80741 912 U80741 GB_PL1: BNDNATRNA 1-732 X89901 GB PR3: HSU80741 912 U80741 rxa00109 735 1- 3 G5S9: AQ153 ~ 2i and AQ1Í3721 GB_HTG4: AC007054 171979 AC007054 GB_HTG4: AC007054 171979 ACC07054 rxa00215 1449 GB_BA1: SC9C7 31360 AL035161 GB_BA1: SCE94 38532 AL049628 GB_BA2: AF110185 20302 AF110185 rxa00289 1299 GB_EST6: N80167 384 N80167 GB_STS: G37084 384 G37084 GB_STS: G37084 384 G37084 rxa00404 2439 GB_BA1: MTCY22D7 31859 Z83866 GB_BA1: ECU82598 136,742 U82598 GB_BA2: AE000165 12003 A 000165 rxa00479 2313 GB_BA1: SCF43A 35437 AL096837 GB_GSS2: CNS015U4 1036 AL1C5910 GB_PR3: HSA494016 50502 AL117328 rxa00497 420 GB_BA1: MTCY78 33818 Z77165 GB_BA2: AF079544 817 AF0"'9544 GB_BA1: MTGR0? 0P 2987 X60350 rxa00575 rxa00599 510 GB_GSS10: AQ199703 439 AQ199703 GB_PR2: AC002127 144165 AC002127 GB_STS: G51234 439 G51234 rxa00600 1221 GB_ BA1: MTCY441 35187 Z80225 GB_BA1: MSGY223 42061 A: OOI9 GB_BA1: BSUB0014 213420 Z99117 rxaOCOS G3 5A2: FG? 9O7O £. 1/0 GB_BA1: OVA "1845 X82176 GB_BA1: SC2G5 38404 AL035478 rxa00648 1533 GB_HTG1: HS74016 169,401 AL110119 GB_HTG1: HS74016 169,401 AL110119 GB_HTG1: HS74016 169,401 AL110119 rxa00764 1239 GB_ ST36: AI898007 609 AI898007 GB_BA2: PAU93274 8008 U93274 GB_BA2: PAU93274 8008 U93274 rxa00803 1353 GB_IN2: CELH34C03 27748 AF100662 GB_HTG2: AC007905 100722 AC007905 GB_HTG2: AC007905 100722 AC007905 rxa00810 324 GB_BA1: TY15C10 33050 Z95436 GB_BA1: MLCB2548 38916 AL023093 GB_BA1: EC0UW76 225419 U00039 rxa00829 2463 GB_BA1: SC5C7 41906 AL031515 GB_BA1: SC5F2A 40105 AL049587 GB BA1: STMDRRS 3374 L76359 rxa00843 468 GB_BA1: MTCY9C4 15916 Z77250 GB_BA1: MTCY9C4 15916 Z77250 rxa00858 568 GB_BA1: SCC54 30753 AL035591 GB_EST18: N96610 547 N96610 GB_ ST18: 5493 436 T45493 rxa00886 1269 GB_BA1: SYCSLLLH 132106 D64006 GB_BA1: SCDNAJ 5611 X77458 G3 3A1: 5TMDNAK L46"00 rxa00900 975 GB_BA2:? COUW67_0 110000 U18997 GB_3A2:? COU 67_0 110000 U18997 GB_BA2: A? 000393 10516 AE000393 rxa00901 537 GB_HTG3: AC010757 175571 AC010757 GB_HTG3: AC010757 175571 AC010757 GB_HTG3: AC011283 87295 AC011283 rxa00981 753 GB_OV: GGA2 5664 512 AJ245664 GB_PL2: AC007887 159434 AC007887 GB_GSS1: CNS00RNW542 542 AL087338 rxa00995 864 GB_ ST29: AI553951 450 AI553951 GB_PR3: AC003029 139166 AC003029 GB_BA1: EAY14603 4479 Y14603 rxa00996 864 GB_BA2: A 00100I 10730 AE001001 GB_EST30: AV018764 242 AV018764 GB_GSS3: B24189 377 B24189 rxaOlOlO 1242 GB_OV: AF007068 356 AF007068 GB_EST10: AA166324 514 AA166324 GB_EST7: W89968 46 W89968 rxa01051 732 GB_GSS12: AQ381423 579 AQ381423 GB_HTG6: AC010901 206,121 AC010901 GB_GSS5: AQ746932 837 AQ746932 rxa01052 432 GB_IN1: CELC13D9 43437 AF016420 GB_IN1: CELC13D9 43487 AF016420 rxa01053 543 GB_0V: CHKMAFG1 1316 D28601 G3 HTG6: AC 010,755 - C -i -z 0 AC010765 GB__HTG6: AC010765 146468 AC010765 rxa01054 612 GB_ _PL1: D45425 962 PHNPNGLP GB_ _HTG2: 170302 AL049553 HSJ402N21 GB_ _HTG2: 170302 AL049553 HSJ402N21 rxa01217 723 GB_ _IN2: AF016688 29784 CELF18A12 GB_ _IN2: AF016688 29784 CELF18A12 GB_ _RO : MUSMCFTR 6304 M60493 rxa01320 1770 GB_ _BA2: AF031037 1472 AF031037 GB_ _HTG1: PFMAL13PA80518 AL109815 GB_ _HTG1: PFMAL13PA80518 AL109815 rxa01345 1575 GB_ _PR3: AC005224 166687 ACC05224 GB_ _PR3: AC005224 166687 ACC05224 GB_ _HTG3: AC011500_1300851 ACC11500 rxa01407 1014 GB_ _HTG3: AC010831 70233 AC010831 GB_ _HTG3: AC010831 70233 AC010831 GB_ _PR3: AC004058 38400 AC004058 rxa01408 324 GB_ _PR4: AF152365 246546 AF152365 GB_ _HTG3: AC007890 121256 AC007890 GB_ _HTG3:: AC007890 121256 AC007890 rxa01524 1566 GB_ _BA1: BSUB0015"218410 Z99118 GB_ _HTG2: AC008260 107439 ACC 08260 GB_ _HTG2: AC008260 107439 ACG 03260 rxa01578 1510 GB_ _PR4: AF111170 143083 AF111170 GB PR4: AF111170 178083 AF1111 ~ 3 Ga 3A1 : A.Y13732 0/4. rxa01616 1605 GB__BA2: AF088857 2908 AF088857 GB_ _IN1: C? M04D8 21552 GB32682 GB_ _? ST25: AI281910 276 AI281910 rxa01666 1500 GB_ _BA1: CGU43535 2531 U43535 GB_ _HTG3: AC009213 114735 AC009213 GB_ _HTG3: AC009213 114735 AC009213 rxa01674 1017 GB_ _PL1: AB017159 1859 AB017159 GB_ _PR1: HUMGN0S48231 2 D26607 GB_ _HTG3: AC011234 154754 AC011234 rxa01873 1359 GB_ _HRG3: AC009450 124337 AC009450 GB_ _HRG3: AC009450 124337 AC009450 GB_ _HTG3: AC009919 134724 AC009919 rxa01922 1275 GB_ _BA1: ECONEUC 1676 M84026 GB_ _HTG2: AC007853 116280 AC007853 GB_ _HTG2: AC007853 116280 AC007853 rxa01936 1395 GB_ _HTG4: AC010037 166249 AC010037 GB_ _HTG4: AC010037 166249 AC010037 GB_ _PR4: AC005552 167228 AC005552 rxa01984 420 GB_ _PR1: HS169C8F 245 Z57239 GB_ _BA1: S? RATTBXIS 3255 L11597 GB EST7: W97557 267 W97557 rxa02060 rxa02087 1470 GB_PR3: AC005544 169045 AC005544 GB PL1: ATF20B18 104738 AL049483 599C-. Allo, 91": xa02088 1338 GB_HTG3: AC008697 AC008697 GB_HTG3 167932: 167932 AC008697 AC008697 GB_HTG3: AC008703 AC008703 rxa02159 636 213971 GB_BA2: AF049897 AF049897 GB_BA2 9196: AF031518 AF031518 GB_BA2 2045: AF041436 AF041436 rxa02184 516 504 GB_BA1: 8164 BSZ92953 Z92953 GB_EST36: AI878071 593 AI878071 GB_EST37: AI958166 641 AI958166 rxa02200 1233 GB_PR3: HSA494016 50502 AL117328 GB_HTG2: AC008161 158440 AC008161 GB_HTG2: AC008161 158440 AC008161 rxa02201 486 GB_EST4: H16949 465 H16949 GB_EST4: H16949 465 H16949 rxa02202 762 GB_IN1: CELC41A3 37149 U41541 GB_EST33: AV080151 236 AV080151 GB_GSS5 : AQ766877 545 AQ766877 rxa02205 1002 GB_HTG2: AC005959 127587 AC005959 GB_HTG2: AC005959 127587 AC005959 GB_IN1: BRPTUBBA 4571 M36380 rxa02305 975 GB_RO: MUSPAFR 1140 D50872 GB_PR3: HUMARL1A 1108 L28997 GB_BA1: MLCB2533 40245 AL035310 rxa02431 899 GB ST4: H35255 407 H35255 G3 pl. echo GB_HTG1: HS791K14 155318 AL035685 rxa02446 558 GB_BA2: AF036166 895 AF036166 GB_EST5: N25122 620 N25122 GB_? ST5: N251 22 620 N25I22 rxa02541 1308 GB_BA2: dDPU93358 1267 U93358 GB_EST3O: AI658096 343 AI658096 GB_EST37: AI959242 545 AI959242 rxa02542 777 GB_PAT:? 10832 1856? 10832 GB_EST24: Z82017 396 Z82017 GB_0M: CATERYTHR0681 L10606 rxa02543 1977 EM_PAT:? 10832 1856 E10832 GB_BA1: MPHSP70 2179 X59437 GB_BA1: MTY13E10 35019 Z95324 rxa02586 393 GB_IN2: AC006472 156,362 AC006472 GB_HTG4: AC010020 106541 AC010020 GB_HTG4: AC010020 106541 AC010020 rxa02587 2214 GB_BA1: MLCL622 42498 Z95398 GB_RO: AF074879 3316 AF074879 GB_RO: RNJ001380 2641 AJ001380 rxs03217 331 GB_BA1: MLCB2548 38916 AL023093 GB_HTG2: HSJ662M14 174772 AL079336 GB_HTG2: HSJ662M14 174772 ALC ^ 9336 ID # Genbank result name rxa00062 Homo sapiens, clone NH0501G22, *** SEQUENCING? ro IN PROGRESS * - > • -, 3 parts not crder.aias. rxa00084 Homo sapiens CAGH44 APNm, partial cds. B. nigra AD? for tAR gene ?. Homo sapiens, CAGH44 AR? M, partial cds. rxa00109 HS_2245_A1_F07_MF CIT Approved Human Genomic Sperm Library D Genomic clone of Homo sapiens plate = 2245 col = 13 row = K, genomic research sequence. Drosophila melanogaster chromosome 2 clone BACR45018 (D527) RPCI-98 45.0.18 map 41E-41? strain and; cn bw sp, *** SECURING IN PROGRESS ***, 13 non-ordered parts. Drosophila melanogaster chromosome 2 clone BACR45018 (D527) RPCI-98 45.0.18 map 41E-41? strain and; cn bw sp, *** S? CU? NTOAMN? N PROGR? SO ***, 13 non-ordered parts. rxa00215 Streptomyces coelicolor cosmid 9C7. Streptomyces coelicolor cosmid? 94. Burkholderia pseudomallei strain 1026b genes of DbhB (dbhB), protein D of general secretory pathway (gspD), protein? of general secretory pathway (gsp?), of protein F of the general secretory route (gspF), GspC (gspC), of protein G of secretory general route (gspG), of protein H of secretory general route (gspH), of protein I of general secretory route . gsplj, ae prctein J ie general secretory pathway (gspJ), protein K of general secretory pathway (gspK), protein L of general secretory pathway (gspL), protein M of general secretory pathway (gspM), and protein N of general secretory pathway (gspN), complete cds; and unknown genes. rxa00289 za65g02.sl Soares fetal spleen liver 1NFLS clone of 7ADNc from Homo sapiens IMAG ?: 297458 3 ', mRNA sequence. SHGC-56832 STS from human genomic Homo sapiens, sequence marked site. SHGC-56832 STS from human genomic Homo sapiens, sequence marked site. rxa00404 Mycobacterium tuberculosis H37Rv, complete genome; segment 133/162. ? scherichia coli genomic sequence of 9 minutes a 12. Escherichia coli K-12 MG1655 section 55 of 400 of the complete genome. rxa00479 Streptomyces coelicolor cosmid F43A. Drosophila melanogaster, extreme SP6 genomic research sequence from BAC BAC 14G08 from Dros3AC library of Drosophila melar.ogaster (fruit fly), genomic research sequence. chromosome 22, complete sequence. rxa00497 Mycobacterium tuberculosis H37Rv, complete-segment genome 145/162. Mycobacterium avium, GroESL operon, partial sequence. M. tuberculosis, gen gro? for KCS and 10-kDa products. rxa00575 rxa00599 RPCI11-46013. TJ RPCI-11 genomic clone of Homo sapiens, RPCI-11-46013, genomic research sequence. Human BAC clone RG305H12 of 7q21, complete sequence. SHGC-80708 Human genomic STS from Homo sapiens, marked sequence site. rxa00600 Mycobacterium tuberculosis H37Rv, complete genome; segment 118/162. Mycobacterium tuberculosis clone sequence y223. Bacillus subtilis complete genome (section 14 of 21): from 2599451 to 2812870. rxa00605? Ndosimbionto of catalase gene of Onchocerca volvulus, complete cds. Onchocerca volvulus, endobacterianc mRNA for catalase. Streptomyces ceelicoior cósmiao 2G5. rxa00648 Homo sapiens, chromosome 21 clone RPCIP704O1674 map 21q21, *** S? CU? NCIAMI? NTO PROGRESO ***, in unordered parts. Homo sapiens, chromosome 21 clone RPCIP704O1674 map 21q21, *** S? CU? NCIAMIENTO IN PROGRESS ***, in unordered parts. Homo sapiens, chromosome 21 clone RPCIP704O1674 map 21q21, *** SECU? NCIAMI? NTO IN PROGRESS ***, in unordered parts. rxa00764 EST267450 tomato ovary, TAMU Lycopersicon esculentum cDNA clone CLED31K22, mRNA sequence. Pseudomonas aeruginosa genes YafE (yaf?), LeuB (leuB), Asd (asd), FimV (fimV), and HisT (hisT), complete cds; TrpF gene (trpF), partial cds; and unknown. Pseudomonas aeruginosa genes YafE (yafE), LeuB (leuB), Asd (asd), FimV (fimV), and HisT (hisT), complete cds; TrpF gene (trpF), partial cds; and unknown. rxa00803 Caenorhabiditis elegans cosmid H34C03. Homo sapiens chromosome 16q24.3 clone PAC 754F23, *** SECUE? CIAMIE? TO E? PROGRESS ***, 33 non-ordered parts. Homo sapiens chromosome 16q24.3 clone PAC 754F23, *** SECUE? CIAMIE? TO E? PROGPE? C -, 33 parts nc ordered. rxa00810 Mycobacterium tuberculosis H37Rv, complete genome; segment 154/162. Mycobacterium leprae cosmid B2548. E. coli chromosomal region from 76.0 to 81.5 minutes. rxa00829 Streptomyces coelicolor cosmid 5C7. Streptomyces coelicolor cosmid 5F2A. Streptomyces peucetius protein gene for daunorubicin resistance (dic), complete. rxa00843 Mycobacterium tuberculosis H37Rv, complete genome; segment 113/162. Mycobacterium tuberculosis H37Rv, complete-segment genome 113/162. rxa00858 Streptomyces coelicolor cosmid C54. 21285 Lambda-PRLl Arabidopsis thaliana clone cDNA F10G3T7, mRNA. 8756 Lambda-PRL2 Arabidopsis thaliana cDNA clone 133C14T7, mRNA. rxa00886 Synechocystis sp. PCC6803 complete genome, 25/27, 3138604-3270709. 'S. coelicolor genes dnak, grpE and dnaJ. Streptomyces coelicoior (strain A3 (2)), dnaK operon encoding molecular chaperones (dnaK, dnaK), grp genes and hspR, complete cds. rxa00900 E = c erichia ccii K-12 regiór. crcmescitai from 6 ~ .4 to 76.0 minutes. Escherichia coli K-12 chromosomal region of 67.4 a 76. 0 minutes Escherichia coli K-12 MG1655 section 283 of 400 of the complete genome. rxa00901 Homo sapiens chromosome 18 clone 128_C_18 map 18, *** SECU? NTOAMI? NTO PROGRESO ***, 20 non-ordered parts. Homo sapiens chromosome 18 clone 128_C_18 map 18, *** SECU? NCIAMIENTO EN PROGRESO ***, 20 unordered parts. Homo sapiens clone MS2016A09, * * * SECU? NCIAMI? NTO EN PROGRESS ***, 1 part not ordered. rxa00981 Gallus gallus partial mRNA for ATP-citrate lyase (ACL gene). Genomic sequence for Arabidopsis thaliana BAC F1504 of chromosome I, complete sequence. Arabidopsis thaliana genomic research sequence T7 end of BAC F14D7 from IGF library from the Columbia strain of Arabidopsis thaliana, genomic research sequence. rxa00995 te54d01.xl Soares_NFL_T_GBC_Sl Homo sapiens cDNA clone IMAGE: 2090497 3 'similar to gb: X02067 H. sapiens mRNA for 7SL RNA pseudogene (HUMAN); Homo sapiens, chromosome 12q24 PAC RPCI3-462E2 (Roswell Park Cancer Institute Human Library PAC), complete sequence. Erwinia amylovora genes srlA, srl ?, srlB, srlD, srlM and srlR. rxa00996 Archaeoglobus fulgidus section 106 of 172 of the complete genome. AV018764 Mus musculus 18-day embryo C57BL / 6J, cDNA clone of Mus musculus 1190006M16, mRNA sequence. F19? 16TF IGF Arabidopsis thaliana, genomic clone F19? 16, genomic research sequence. rxaOlOlO Coturnix coturnix, arylalkylamine mRNA N-acetyltransferase, partial cds. ms50c09.rl Life Tech Mouse Embryo 13 5dpc 10666014 Mus musculus cDNA, IMAG clone: 614992 5 'similar to SW: NEST_RAT P21263 NESTIN; sequence of MRNA. mf64gll.rl mouse embryo Soares NbME13.5 14.5 cDNA clone of Mus musculus IMAGE: 419108 5 'similar to SW: NEST_RAT P21263 N? STIN. [1],; mRNA sequence. rxa01051 RPCI11-135F10. TJ RPCI-11 genomic clone of Homo sapiens RPCI-11-135F10, genomic research sequence.

Homo sapiens clone RP11-544J22, S? CU? NCIA D? DRAFT D? WORK, 1 part not ordered. HS_5538_A1_A11_T7A RPCI-11 Human Male BAC Library genomic clone of Homo sapiens, plate = 1114 col = 21 row = A, genomic research sequence. rxa01052 Caenorhabiditis elegans cosmid C13D9. Caenorhabiditis elegans cosmid C13D9. rxa01053 Maf-related gene with novel chicken maf that encodes bZip MafG nuclear protein, promoter region and exon 1. Homo sapiens, clone RP11-115N6, *** SEQUENCING ? N PROGR? SO ***, 26 unordered parts. Homo sapiens, clone RP11-115N6, *** S? CUENCIAMIENTO ? N PROGR? SO ***, 26 unordered parts. rxa01054 Pharbitis nil mRNA for precursor of germinal protein of Pharbitis nil, complete cds. Homo sapiens, chromosome 6 clone RP3-402? 21 map p21.l-21.31, *** S? CU? CIAMI ?? TO E? PROGRESS ***, in unordered parts. Homo sapiens, chromosome 6 clone RP3-402? 21 map p21.l-21.31, *** SECU ?? CIAMIE? TO ?? PROGRESS ***, in unordered parts. rxa01217 Caenorhabiditis elegans cosmid F18A12. Caenorhabiditis elegans cosmid F18A12. AR? M ie controller of conductivity ie trar.smeiicrana of cystic fibrosis of mouse (CFTR), complete cds. rxa01320 Neisseria meningitidis chloramphenicol acetyltransferase gene, complete cds. Plasmodium faiciparum, chromosome 13 strain 3D7, *** SECU? NCIAMIENTO EN PROGRESO ***, in unordered parts. Plasmodium falciparum, chromosome 13 strain 3D7, *** SECU? NCIAMIENTO? N PROGR? SO ***, in unordered parts. rxa01345 Homo sapiens chromosome 17, clone hRPK.214_0_l, complete sequence. Homo sapiens chromosome 17, clone hRPK.214_0_1, complete sequence. Homo sapiens, chromosome 19, clone CIT978SKB_60E11, *** S? CU? NCIAMI? NTO PROGRESO ***, 246 unordered parts. rxa01407 Homo sapiens clone 6_L_24, SEQUENCE SAMPLING OF LOW STEP. Homo sapiens clone 6_L_24, LOW PASSAGE SEQUENCE SAMPLING. Homo sapiens chromosome 4 clone B241P19 map 4q25, complete sequence. rxa01408 Homo sapiens, FRA3B sequence fragile constitutive region. Drosophila melanogaster chromosome 3 clone 3AC ?: 2G21 (D722) RPCI-98 02.G.21 map 90E-91A, strain y; cn bw sp, *** SECU? NCIAMIENTO? N PROGR? SO ***, 89 unordered parts. Drosophila melanogaster chromosome 3 clone BACR02G21 (D722) RPCI-98 02.G.21 map 90? -91A, strain y; cn bw sp, *** S? FOUNDATION IN PROGRESS ***, 89 unordered parts. rxa01524 Bacillus subtilis complete genome (section 15 of twenty-one); from 2795131 to 3013540. Drosophila melanogaster chromosome 2 clone BACR13J10 (D924) RPCI-98 13.J.10 map 47B-47C, strain y; cn bw sp, *** SECRET IN PROGRESS ***, 82 non-ordered parts. Drosophila melanogaster chromosome 2 clone BACP13J10 (D924) RPCI-98 13.J.10 map 47B-47C, strain y; cn bw sp, *** SECU? NCIAMIENTO EN PROGRESO ***, 82 non-ordered parts. rxa01578 Homo sapiens 14q32 gene Jagged2, complete cds; and unknown gene; Homo sapiens 14q32 gene Jagged2, complete cds; and unknown gene; Alcaligenes eutrophus genes for ureases, _reDl, ureD2, ureA, ureB, and ORFl, 0RF2. rxa01616 Vogesellea indigofera locus regulator of Piosynthesis ie ia indigoiama, comp_eta sequence.

Caenorhabiditis elegans cosmid M04D8, complete sequence. qt82d04.xl NCI_CGAP_Col4 cDNA clone of Homo sapiens, IMAG ?: 1961767 3 ', mRNA sequence. rxa01666 Corynebacterium glutamicum, multiple drug resistance protein gene (cmr), complete cds. Drosophila melanogaster chromosome 3 clone BACR09F18 (D812) RPCI-98 09.F.18 map 98D-98D strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 109 non-ordered parts. Drosophila melanogaster chromosome 3 clone BACR09F18 (D812) RPCI-98 09.F.18 map 98D-98D strain y; cn bw sp, *** SECU? NTIAMI? NTO IN PROGRESS ***, 109 non-ordered parts. rxa01674 Daucus carota mRNA for citrate synthase, complete cds. Homo sapiens, nitric oxide endothelial synthase gene, complete cds. Homo sapiens clone NH0166D23, *** SEQUENCING IN PROGRESS ***, 7 non-ordered parts. rxa01873 Homo sapiens, chromosome 9, clone 30_C_23 map 9, *** SECUENCIAMI? NTO? N PROGR? SO + ** 20 non-ordered parts. Homo sapiens, chromosome 9, clone _ _: _ 23 map 9, SEQUENCE IN PROGRESS *** 20 non-ordered parts. Homo sapiens clone 115_I_23, MU? STR? O D? SEQUENCE OF LOW PASSAGE. rxa01922 E. coli protein gene ~ (neuC), complete cds. Drosophila meianogaster chromosome 3 clone BACR03L02 (D766) RPCI-98 03. L.2 map 96B-96C strain y; cn bw sp, *** SECUXING IN PROGRESS *** 80 non-ordered parts. Drosophila melanogaster chromosome 3 clone BACR03L02 (D766) RPCI-96 03. L.2 map 96B-96C strain y; cn bw sp, *** SEQUENCING IN PROGRESS *** 80 non-ordered parts. rxa01936 Drosophila melanogaster chromosome 3L / 66B6 clone RPCI98-6E4, "* SEQUENCE IN PROGRESS *** 52 unordered parts Drosophila melanogaster chromosome 3L / 66B6 clone RPCI98-6E4, * - * SEQUENCE IN PROGRESS *** 52 parts not ordered. Homo sapiens chromosome 17, clone hRPK.212_E_8, complete sequence. rxa01984 H. sapiens ANÍ of island CpG genomic fragment Msel, clone 169c8, reading forward, cpg 169c8.ft la. Saccharopolyeepora erytnraea excisionasa gene (xis), gene ae ir.tegra = a (ir.c., ees ccmp_ecas and site attB.mf98a09.rl Soares mouse embryo NbME13.5 14.5 cDNA clone of Mus musculus IMAGE: 422296 5 ' mRNA sequence rxa02060 rxa02087 Homo sapiens chromosome 17 clone hRPK.349_A_8, full sequence Arabidopsis thaliana AD? chromosome 4, BAC clone F20B18 (ESSA project). Arabidopsis thaliana AD? chromosome 4, BAC (project SSA). rxa02088 Homo sapiens, chromosome 5 clone CIT978SKB_70D3, *** SECURE? CIAMI ?? TO E? PROGRESS ***, 54 non-ordered parts. Homo sapiens, chromosome 5 clone CIT978SKB_70D3, *** SECU ?? CIAMI ?? TO ?? PROGRESS ***, 54 non-ordered parts. Homo sapiens, chromosome 5 clone CIT978SKB_70D3, *** SECUE? CIAMI ?? TO ?? PROGRESS ***, 54 non-ordered parts. rxa02159 Corynebacterium glutamicum? - acetylglutamyl phosphate reductase gene (argC), ornithine acetyltransferase (argJ),? -acetylglutamate kinase (argB), acetylornithine transaminase (argD), ornithine carba oiltransferase ^ argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH), complete cds. Corynebacterium glutamicum ornithine gene carbamoyltransferase (argF), complete cds. Corynebacterium glutamicum, arginine repressor gene (argR), complete cds. rxa02184 B. subtilis genes yws [A, B, C] and rbs genes [A, C, D, K, R]. fc57al2.yl and zebrafish WashU MPIMG EST Danio rerio ARNc 5 'similar to TR: Q13151 Q13151 RIBONUCLEOPROTÍÍNA NUCL? AR HETEROGÉNEA AO: mRNA sequence. fc91f01.yl zebra fish WashU MPIMG EST Danio rerio 5 'cRNA similar to TR: Q13151 Q13151 RIBONUCLEOPROT? NA NUCL? AR HETEROGEN? A AO: mRNA sequence. rxa02200 AND human clone sequence 494016 on chromosome 22, complete sequence. Mus musculus clone 182_H_5, *** SEQUENCING IN PROGRESS ***, 29 unordered parts. Mus musculus clone 182_H_5, *** SEQUENCING IN PROGRESS ***, 29 non-ordered parts rxa02201 ym34all.rl Soares brain of infant 1NIB, cDNA of Homo sapiens, clone IMAGE: 50010 5 'mRNA sequence. ym34all.rl Soares brain of infant 1NIB, cDNA of Homo sapiens, clone IMAGE: 50010 5 'sequence MRNA. rxa02202 Caenorhabiditis elegans cosmid C41A3. AV080151 Mus musculus stomach C57BL / 6J, cDNA from Mus musculus adult clone 2210413B04, sequence of MRNA. HS_2017_B2_B08_MR CIT Approved Human Genomic Sperm Library D genomic clone of Homo sapiens plate = 2017, col = 16 row = D, genomic research sequence. rxa02205 Homo sapiens, *** SECU? NTIAMI? NTO? N PROGR? SO ***, 2 ordered parts. Homo sapiens, *** S? CU? NCIAMI? NTO? N PROGR? SO ***, 2 ordered parts. B. pahangi beta-tubulin gene, complete cds. rxa02305 Mouse gene for platelet activation factor receptor, complete cds. Homo sapiens, ARL1 mRNA, complete cds. Mycobacterium leprae cosmid B2533. rxa02431? ST111890, rat PC-12 cells, Rattus sp. cDNA. treated with NGF (9 days), RPNCO03, sequence of MRNA. Homo sapiens, chromosome 20 clone RP4-791K14, ***? ECU? NCIAMI? XIO IN PROGRESS "~, in orderly parts Homo sapiens, chromosome 20 clone RP4-791K14, *** SECU? NTOAMN? N PROGR? SO ***, in unordered parts. rxa02446 Xanthomonas campestris protein gene for resistance to organic hydroperoxide (ohr), complete cds. yxl9dl0.rl Soares melanocyte 2NbHM, Homo sapiens cDNA clone IMAG ?: 262195 5 ', mRNA sequence. ysc0dl0.rl Soares melanocyte 2NbHM, Homo sapiens cDNA clone IMAG ?: 262195 5 ', rxa02541 mRNA sequence Deinococcus proteolyticus, heat shock chaperone protein gene (dnaJ) of 40 kDa, complete cds. fcl4c09.yl and zebrafish WashU MPIMG? ST Danio rerio 5 'cDNA similar to S: DNJ2_HUMAN P31689 HOMOLOGOUS D? PROTEIN DNAJ 2; mRNA sequence. fd25hll.yl zebra fish WashU MPIMG EST Danio rerio 5 'cDNA similar to S: DN 2_HUMAN P31689 HOMOLOGOUS D? PROTEIN DNAJ 2; mRNA sequence. rxa02542 DNA encoding Dnak protein which is one of the heat shock protein from SSZ82017 porcine small intestine cDNA library, Sus scrofa cDNA clone cl2c06 5 'similar to eukaryotic start factor 4 gamma, ie ie mRNA sequence. Cat erythropoietin mRNA, 3 'end. rxa02543 DNA encoding Dnak protein is one of the heat shock proteins of M. paratuberculosis, gene for 70 kD heat shock protein. Mycobacterium tuberculosis H37Rv, complete genome; segment 18/162. rxa02586 Drosophila melanogaster, chromosome 2R, region 45? 1- 46A2, BAC clone BACR48G21, complete sequence. Drosophila melanogaster, chromosome 3L / 66D10 clone RPCI98-2613, *** S? COUNTING IN PROGRESS ***, 55 unordered parts. Drosophila melanogaster, chromosome 3L / 66D10 clone RPCI98-2613, *** SEQUENCING IN PROGRESS ***, 55 unordered parts. rxa02587 Mycobacterium leprae cosmid L622. Rattus norvegicus TSPY gene of testicular specific protein, complete cds. Rattus norvegicus Tspy partial genomic sequence, exons 1-6. rxs03217 Mycobacterium leprae cosmid B2543. Homo sapiens chromosome 20, clone PP4-662 M14, *** SECUENCLAMÍENTO IN PROGRESO * + *, 10 non-ordered parts.

Homo sapiens chromosome 20, clone RP4-662 M14, *** SEQUENCING? N PROGR? SO ***, 10 non-ordered parts. ID # Source of results% of homology Date of de Genbank (GAP) deposit rxa00062 Homo sapiens 39,080 05-06-1999 rxa00084 Homo sapiens 39,264 18-12-1997 Brassica nigra 36,725 06-02-1997 Homo sapiens 38,957 18-12-1997 rxa00109 Homo sapiens 45,066 16-10-1998 Drosophila melanogaster 36, 589 13-10-1999 Drosophila melanogaster 36, 589 13-10-1999 rxa00215 Streptomyces coelicolor 44, 444 12-01-1999 Streptomyces coelicolor 36, 313 12-04-1999 Burkholderia 44,159 02-08-1999 Pseudomallei rxa00289 Homo sapiens 40,420 29-03-1996 Homo sapiens 40,420 30-03-1998 Homo sapiens 40,420 30-03-1998 rxa00404 Mycobacterium 60,271 17-06-1998 tuberculosis Escherichia coli 54,256 15-01-1997 Escherichia coli 54,256 12-11-1998 rxa00479 Streptomyces coelicolor 36, 245 13-07-1999 AJ 2 Drosophila melanogaster 37, 573 26-07-1999 Homo sapiens 36, 475 23-11-1999 rxa00497 Mycobacterium 40,250 17-06-1998 tuberculosis Mycobacterium avium 64,439 August 16, 1998 Mycobacterium 62,857 23-04-1992 tuberculosis rxa00575 rxa00599 Homo sapiens 42,657 20-04-1999 Homo sapiens 37,052 27-05-1997 Homo sapiens 42,657 25-06-1999 rxa00600 Mycobacterium 56,183 18-06-1998 tuberculosis Mycobacterium 37,217 10-12-1996 tuberculosis Bacillus subtilis 36,553 26-11-1997 rxa00605 endosimbionte 55,396 25-11-1998 Onchocerca volvulus endosimbionte 55,396 26-11 -1998 Onchocerca volvulus Streptomyces coelicolor 39, 530 11-06-1999 rxa00648 Homo sapiens 36,327 27-08-1999 Homo sapiens 36,327 27-03-1999 Homo sapiens 35, 119 27-08-1999 : xaü0764 Lycopersiccn esculentum 34,323 2"-C ~ -1999 Pseudomonas 35,898 23-06- 1998 aeruginosa rxa00803 Caenorhabditis elegans 34,152 28-10-1998 Homo sapiens 37,472 24- 06-1999 Homo sapiens 37,472 24-06- 1998 rxa00810 Mycobacterium 24,615 17- 06-1998 tuberculosis Mycobacterium leprae 34,615 27-08-1999? Scherichia coli 52,997 07- 11- 1996 rxa00829 Streptomyces coelicolor 65, 269 07-09-1998 Streptomyces coelicolor 37, 490 24-05- 1999 Streptomyces peucetius 55,279 24-12-1996 rxa00843 Mycobacterium 40,000 17-06-1998 tuberculosis Mycobacterium 37,773 17-06-1998 tuberculosis rxa00858 Streptomyces coelicolor 39, 602 11-06-1999 Arabidopsis thaliana 37,301 05-01-1998 Arabidopsis thaliana 34,194 04-08-1998 rxa00886 Synechocystis sp. 37,459 13 -02-1999 Streptomyces coelicolor 49, 44 21-11-1996 Streptomyces coelicolor 49, 583 22-11-1996 rxa00900? Scherichia coli 38,314 U18997 Escherichia coli 37, ^ 59 U18997 ZL = cr.erichia col i J c, _ ^ 12 -11-1995 rxa00901 Homo sapiens 34,857 22-09-1 999 Homo sapiens 34,857 22-09-1999 Homo sapiens 35,448 07-10-1999 rxa00981 Gallus gallus 37,538 28-09-1999 Arabidopsis thaliana 37,600 04-10-1999 Arabidopsis thaliana 41,264 28-06-1999 rxa00995 Homo sapiens 42,627 13-04- 1999 Homo sapiens 38,915 17-09-1998 Erwinia amylovora 37,694 06-06-1998 rxa00996 Archaeoglobus fulgidus 41,078 15-12-1997 Mus musculus 39, 669 28-08-1999 Arabidopsis thaliana 44,385 10-10-1997 rxaOlOlO Coturnix coturnix 46,629 12- 07-1997 Mus musculus 38, 677 19-12-1996 Mus musculus 58,696 12-09-1996 rxa01051 Homo sapiens 37,651 21-05-1999 Homo sapiens 36,011 04-12-1999 Homo sapiens 38,640 19-07-1999 rxa01052 Caenorhabditis elegans 39,344 02-08-1997 Caenorhabditis elegans 38,780 02-08-1997 rxa01053 Gallus gallus 39,205 07-02-1999 Homo sapiens 32,961 07-12-1999 Homo sapiens 38,476 07-12-1999 rxa01054 Ipomoea nil 42,925 10-02-1999 rlOi O sapiens J o, Homo sapiens 36,825 03-12-1999 rxa01217 Caenorhabditis elegans 35,794 08-10-1999 Caenorhabditis elegans 4 0, 625 08-10-1999 Mus musculus 37,793 10-06-1994 rxa01320 Neisseria meningitidis 35, 014 21-04-1998 Plasmodium falciparum 17,697 19-08-1999 Plasmodium falciparum 17, 697 19-08-1999 rxa01345 Homo sapiens 38,195 14 -08-1998 Homo sapiens 36,611 14-08-1998 Homo sapiens 36,446 AC011500 rxa01407 Homo sapiens 35,764 23-09-1999 Homo sapiens 35,764 23-09-1999 Homo sapiens 40,778 30-09-1998 rxa01408 Homo sapiens 41,234 01-08-1999 Drosophila melanogater 39,432 03-09-1999 Drosophila melanogater 39,432 03-09-1999 rxa01524 Bacillus subtilis 38,201 26-11-1997 Drosophila melanogater 38,302 02-08-1999 Drosophila melanogater 38,302 02-08-1999 rxa01578 Homo sapiens 37,873 14-07-1999 Homo sapiens 40,220 14-07-1999 Ralstonia eutropha 42,960 23-C9-1997 rxa01616 Vogesella indigofera 37,626 10-09-1999 Caenorhabditis elegans 37,237 23-11-1998 Homo sapiens 33,406 rxa01666 Corynebacterium 99,933 09-04-1997 glutámicum Drosophila melanogaster 36, 111 08-23-1999 Drosophila melanogaster 36, 111 23- 08-1999 rxa01674 Daucus carota 39,537 01-05-1999 Homo Sapiens 36,419 13-07-1999 Homo Sapiens 36,317 04-10-1999 rxa01873 Homo sapiens 35,303 22-08-1999 Homo Sapiens 35, 303 22-08-1999 Homo Sapiens 35,409 08-09-1999 rxa01922 Escherichia coli 35, 189 26-04-1993 Drosophila melanogaster 34,365 02-08-1999 Drosophila melanogaster 34,365 02-08-1999 rxa01936 Drosophila melanogaster 38,534 16-10-1999 Drosophila melanogaster 38, 534 16-10- 1999 Homo sapiens 36,249 26-11-1998 rxa01984 Homo sapiens 45, 679 18-10-1995 Saccharopolyspora 36,232 06-07-1994 Erythraea mus musculus 42,969 16-07-1996 rxa02060 rxa02087 Homo sapiens 33,724 25-09-1998 Arabidopsis thaliana 35,890 24 -03-1999 Arabidopsis thaliana 38,128 27-08-1999 Homo sapiens 55,662 03-06-1999 Homo sapiens 36,662 03-08-1999 Homo sapiens 34,768 03-08-1999 rxa02159 Corynebacterium 99,843 01-07-1998 glutamicum Corynebacterium 88, 679 05 -01-1999 glutamicum Corynebacterium 100,000 05-01-1999 glutamicum rxa02184 Bacillus subtilis 38, 951 24-06- 1998 Danio rerio 36,774 21-07- 1999 Danio rerio 36,774 20-08- 1999 rxa02200 Homo sapiens 38, 648 23-11- 1999 Mus musculus 35,938 28-07- 1999 Mus musculus 35, 938 28-07 - 1999 xa02201 Homo sapiens 38,267 29-06- 1995 Homo sapiens 36,552 29-06- 1995 rxa02202 Caenorhabditis elegans 41,678 08-12- 1995 Mus musculus 43,348 25-06- 1999 Homo sapiens 35,568 28-07- 1999 rxa02205 Homo sapiens 40,310 11- 11- 1998 Homo sapiens 10,310 11-11- 1998 Brugia pahangi 37,703 26-54- 199 3 rxa02305 Mus musculus 38,420 10-02- 1999 Homo sapiens 42, 188 13-01- 1995 Míycobacterium leprae 42,000 rxa02431 Rattus sp. 39,098 02-04-1998 Homo sapiens 39,456 23-11-199 Homo sapiens 39,456 23-11-1999 rxa02446 Xanthomonas campestris 49,369 19-05-1998 Homo sapiens 35,417 28-12-1995 Homo sapiens 37, 172 28-12-1995 rxa02541 Deinococcus 42,115 17-01-1998 proteolyticus 42,115 17-01-1998 Danio rerio 52,059 06-05-1999 Danio rerio 45,438 08-08-1999 rxa02542 Corynebacterium 99,000 08-10-1997 glutamicum (Rei 52, created) Sus scrofa 37, 067 04-30-1999 Felis catus 39,409 14-10-1993 rxa02543 Corynebacterium 97,306 08-10-1997 glutamicum Mycobacterium avium 73,404 23-04-1992 Subsp. Paratuberculosis Mycobacterium 72,028 17-06-1998 tuberculosis rxa02586 Drosophila melanogaster 37,958 30-01-1999 Drosophila melanogaster 37, 333 16-10-1999 Drosophila melanogaster 37,333 16-10-1999 Mycobacterium ieprae 39,646 24-56-1997 Rattus norvegicus 35,830 06-07 -1999 Rattus norvegicus 37,702 29-06-1998 rxs03217 Mycobacterium leprae 37,888 27-08-1999 Homo sapiens 36,420 04-02-2000 Homo sapiens 35,962 04-02-2000 EXAMPLES Example 1: Preparation of total genomic DNA of Corynebacterium gl utamicum ATCC 13032 A culture of Corynebacterx um gl utami cum (ATCC 13032) was grown overnight at a temperature of 30 ° C with vigorous shaking in BHI medium (Difco). The cells were harvested by centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml of buffer-I (5% of the original volume of the culture - all indicated volumes have been calculated for 100 ml of culture volume). Composition of the buffer-I: 140.34 g / 1 sucrose, 2.46 g / 1 MgSO x 7H20, 10 ml / L of a solution of KH2P04 (100 g / 1, adjusted to pH 6.7 with KOH), 50 ml / 1 M12 concentrate (10 g / 1 (NH4) 2S0, 1 g / 1 NaCl, 2 g / l MgSO4 x 7H0, 0.2 g / 1 CaCl;, 0.5 g / 1 of yeast extract (Difco), 10 ml / 1 mixture of minor elements (200 mg / l FeSO- x H_0, 10 mg / l ZnS04 x 7H: 0.3 mg / l MnCl2 x 4H20, 30 mg / l H; BO ^ 20 mg / l CoCl2 x 6H.0, 1 mg / l NiCl ^ x 6H_0, 3 mg / l NaMoO x 2H_C, 500 mg / l complexing agent (EDTA or acid critical), 100 mi / i of vitamin mixture \ .2 g / l of biotin, 0.2 mg / l of folic acid, 20 mg / l of p-aminobenzoic acid, 20 mg / l of riboflavin, 40 mg / l of ca-pantothenate, 140 mg / l of nicotinic acid, 40 mg / l of pyridoxol hydrochloride, 200 mg / l of io-inositol). Lysozyme was added to the suspension at a concentration of 2.5 mg / ml. After an incubation of -about 4 hours at a temperature of 37 ° C, the wall of the cells was degraded and the resulting protoplasts were harvested by centrifugation. The pellet was washed once with 5 ml of buffer-I and once with 5 ml of buffer-TE (Tris-HCL, 10 mM, EDTA 1 mM, pH 8). The pellet was resuspended in 4 ml of TE buffer and 0.5 ml of an SDS solution (10%) and 0.5 ml of a NaCl solution (5 M) were added. After adding proteinase K to a final concentration of 200 μg / ml, the suspension is incubated for about 18 hours at a temperature of 37 ° C. The DNA was purified by extraction with phenol, phenol-chloroform-alcoholisoamyl and chloroform-alcoholisoamyl using procedures standards Then, the DNA was precipitated by addition of 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 minute incubation at a temperature of -20 ° C and a 30 minute centrifugation at 12,000 rpm in a high speed centrifuge using an SS34 rotor (Sorvall). The DNA was dissolved in 1 ml of TE buffer containing 20 μg / ml RNaseA and purified at a temperature of 4 ° C against 1000 ml of TE buffer for at least 3 hours. During this period of time, the shock absorber was changed 3 times. To 0.4 ml aliquots of the dialysed DNA solution, 0.4 ml of 2M LiCl and 0.8 ml of ethanol were added. After an incubation period of 30 minutes at a temperature of -20 ° C, the DNA was collected by centrifugation (13,000 rpm, Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in TE buffer. The DNA prepared by this method can be used for all purposes, including Southern staining or construction of genomic libraries. Example 2: Construction of genomic libraries in Escherichia colx of Corynebacterxumum utamx cum ATCC13032 Using the DNA prepared in accordance with that described in Example 1, cosmid and plasmid libraries were constructed in accordance with well-known and well-established methods (see, as for example, Sambrook, JJ Et al. (1989) "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", "John Wiley & Sons).

Any plasmid or cosmid can be used. Plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Nati Acad. Sci. USA, 75: 3737-3741); pACYC177 (Change &Cohen (1978) J. Bacteriol 134: 1141-1156), plasmids of the p3S series (pBSSK +, pBSSK- and others;? Tratagene, LaJolla, United States) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53: 283-286). Gene libraries specifically for use C. gl utamicum can be constructed using the plasmid pSL109 (Lee, H.-S. and A.J. Sinskey (1994) J. Microbiol, Biotechnol 4: 256-263). ? j 3: DNA sequencing and computational functional analysis Genomic libraries according to Example 2 were used for DNA sequencing in accordance with standard methods, particularly through the chain termination method using AB137 sequencing machines ( see, eg, Fleischman, RD et al., (1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269: 496-512." Sequencing primers with the following "nucleotide sequences" were employed: 5'-GGAAACAGTATGACCATG-3 '(S? Q ID NO: 305) or 5'-GTAAAACGACGGCCAGT-3' (S? Q ID NO: 306) Example 4: Mutagenesis in vivo The mutagenesis in vivo of Corynebacterium glutamicum can be effected by the passage of plasmid DNA (or another vector) through E coli or other microorganisms (for example Bacillus spp, or yeasts such as Saccaharomyces cerevisiae) that are affected in terms of their cap to maintain the integrity of your genetic information. Typical mutant strains have mutations in the genes for the DNA repair system (eg, mutHLS, utD, mutT, etc., for reference, see Rupp, WD (1996) DNA repair mechanisms, in: Escheri chia colx and Salmonella, P. 2277-2294, ASM: Washington.). Such figures are well known to people with ordinary knowledge of the subject. The use of such strains is illustrated, as for example in Greener, A. and Callahan, M. (1994) Strategies /: 32-34. • Example 5: DNA transfer between? scherichia coli and Corynebacterium glutamicum Several species of Corynebacterxum and Brevxbacterium contain endogenous plasmids (such as pHM1519 or pBLI) that replicate autonomously (for a review see Martín, JF et al. (1987) Biotechnology, 5: 137-146). Vectors of "shuttle" type for Escheri chia colx and Corynebacteri um gl utamicum can be easily constructed using standard vectors for E. coii (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, FM et al. (1994) "Current Protocols in Molecular Biology", John Wiley & amp;; Sons) to which is added an origin of replication for Corynebacterxum glutamxcum and an adequate marker of Corynebacterium um gl utamx cum. Such sources of application are preferably taken from parasitic enzogens isolated from Corynebacterium um and Brevibacterium species. Particularly useful as transformation markers for these species are kanamycin resistance genes (such as transposon derivatives Tn5 or Tn903) or chloramphenicol (Winnacker, EL (1987) "From Genes to Clones-Introduction to Gene Technology, VCH, Weinheim) There are numerous examples in the literature of a construction of a wide variety of "shuttle" vectors that replicate in both E. coli and C. gl utamicum, and that can be created for various purposes, including overexpression of genes (for reference see for example, Yoshihama, M. et al. (1985) J. Bacteriol 162: 591-597, Martin JF et al. (1987) Biotechnology, 5: 137-146 and Eikmanns, BJ et al. (1991) Gene, 102: 93-98) Using standard methods, it is possible to clone a gene of interest in one of the "shuttle" vectors described above and introduce said hybrid vectors into strains of Corynebacterium um gl utami cum. transformation of C. gl utamicum can l ograrse by protoplast transformation (Kamata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53: 399-303) and in which case special vectors are used, also by conjugation (in accordance with that described in Schafer A et al., 1990) J. Bacteriol. 172: 1663-1666). It is also possible to transfer the shuttle vectors for C. gl utamicum to E. colx by preparing plasmid DNA from C. gl utamicum (using standard methods well known in the art) and transforming it into E. This transformation step can be employed using standard methods, but it is advantageous to use an E. coli strain deficient in Mcr such as NM522 (Gough &Murria (1983) J. Mol. Biol. 166: 1-19) . Genes can be over-exposed in strains in C. gl utamicum using plasmids comprising pCGl (US Patent No. 4,617,267) or fragments thereof, and optionally the gene for kanamycin resistance of TN903 (Grindley, ND and Joyce, CM (1980) Proc. Nati, Acad. Sci. United States 77 (12): 7176-7180). In addition, genes can be overexpressed in strains of C. gl utamxcum using plasmid pSL109 (Lee, H.-S. and A. J. Sinskey (1994) J. Microbiol, Biotechnol.4: 256-263). Apart from the use of replicative plasmids, the overexpression of genes can also be achieved by integration into the genome. Genomic integration in C. gl utamicum or other species of Corynebacterium or Brevibacterium can be achieved through well-known methods such as homologous recombination with genomic region (s), median integration by restriction endonuclease (REMI) ( see, for example, German Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions (eg, a promoter, a repressor and / or an enhancer) by sequence modification, insertion or removal using site-directed methods (homologous recombination) or methods based on random events (such transposon mutagenesis or REMI). Nucleic acid sequences that function as transcriptional terminators can also be inserted 3 'in relation to the coding region of one or several genes of the invention; such terminators are well known in the art and described, for example, in Winnacker,? .L. (1987) From Genes to Clones-Introduction to Gene Technology. VHC: Weinheim. Example 6: Evaluation of Mutant Protein Expression Observations of the activity of a mutated protein in a host cell are based on the fact that the mutant protein is expressed similarly and in a similar amount as the wild-type protein. A useful method for determining the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot analysis (for reference, see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York), wherein an initiator designed to bind to the gene of interest is labeled with a detectable marker (usually radioactive or chemiluminant) in such a way that when the total RNA of a culture of the organism is extracted, the matter in gel, transferred to a stable matrix and incubated with this probe, the binding and amount of binding of the probe indicate the presence and also the amount of mRNA for this gene. This information is evidence of the degree of transcription of the mutant gene. The total cellular RNA can be prepared from Corynebaz zerxum cum utami cum by several methods, all well known in the art, such as for example the method described in Bormann, E.R. et al. (1992) Mol. Microbiol. 6: 317-326. To evaluate the presence or relative amount of protein translated from this mRNA, standard techniques such as Western blot analysis can be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley:? Ew York ). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, incubated with a probe, such as for example an antibody, which binds specifically with the desired protein. This probe is usually labeled with a chemiluminium or calorimetric marker that can be easily detected. The presence and amount of marker observed indicates the presence or amount of the desired mutant protein present in the cell. ? g 7: Cultivation of genetically modified Corynebacterium glutamicum - Genetically modified Corynebacterxa culture media and conditions are grown in synthetic or natural culture media. Numerous different culture media for Corynebacterxa are well known and readily available (Lieb et al (1989) Appl. Microbiol. Biotechnol., 32: 205-210; von der Osten et al. (1998) Biotechnology Letters, 11: 11-16; German Patent 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al. Springer-Verlag editions.) These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and minor elements.The preferred carbon sources are sugars, such as, for example, monosaccharides, disaccharides or polysaccharides, eg, glucose, fructose, mannose, galactose, ribose, sorbose, rubulose, lactose, maltose, sucrose, raffinose, starch or cellulose. It is also possible to supply sugar to the media through complex compounds such as molasses or other sugar refining byproducts It can also be useful to supply mixtures of different carbon sources Other possible sources of carbon are alcohols and organic acids such as methanol, ethanol, acetic acid or lactic acid Nitrogen sources are usually organic or inorganic nitrogen compounds or materials that contain these compounds. Exemplary sources of nitrogen include ammonia gas or ammonia salts such as NH4C1 or (NH4) 2S0, NH4OH, nitrates, urea, amino acids or complex nitrogen sources such as corn distillation liquor, soybean meal, soy protein, extract of yeast, meat extract and others. Compounds of inorganic salts that may be included in the media include the chloride, phosphorous or sulfate salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc and metal. Chelation compounds can be adhered to a metal medium in solution. Particularly useful chelating compounds include dihydroxyphenols, such as for example catechol or protocatequate, or organic acids such as citric acid. It is typical that the media also contains other growth factors such as vitamins or growth promoters, examples of which include biotin, riboflavin, tiamma, folic acid, nicotinic acid, pantothenate and pyridoxine. Growth factors and salts are frequently originated from components of complex media such as yeast extract, molasses, corn distillation liquor and others. The exact composition of the compounds of the media depends strongly on the immediate experiment and is determined individually for each specific case. Information regarding media optimization is available in the book "Applied Microbiol Physiology, A Practical Approach (PM Rodees editions, PF Stanbury, IRL Press (1997) pp. 53-73, ISBN 019 963577 3). It is possible to select growth media from commercial suppliers, such as standard 1 (Merck) or BHI (infusion, DIFCO) or others.All medium components are sterilized, either by applying heat (20 minutes at 1.5 bar and 121 ° C) or through sterile filtration All components can be sterilized either together or, if necessary, separately All media components can be present at the beginning of growth, or they can be added optionally continuous or in batches.The culture conditions are defined separately for each experiment.The temperature must be within a range between 15 ° C and 45 ° C. be kept constant or it can be altered during the experiment. The pH of the medium should be within the range of 5 to 8.5, preferably about 7.0, and can be maintained by the addition of buffers to the medium. An exemplary buffer for that purpose is a potassium phosphate buffer. Synthetic shock absorbers such as MOPS, HEP? S, AC? S and others can be used alternatively or simultaneously. It? s also possible to maintain a constant culture pH through the addition of NaOH or NH4OH during growth. If complex medium components such as yeast extract are used, the need for additional dampers can be reduced due to the fact that many complex compounds have high damping capacities. If a fermenter is used to grow the microorganisms, the pH can also be controlled using gaseous ammonia. The incubation time is usually within a range of several hours to several days. This time is selected to allow the accumulation of the maximum amount of product in the broth. The growth experiments disclosed can be carried out in various containers such as microtitre plates, glass tubes, glass jars, or glass or metal containers of different sizes. For the screening of a large number of clones, the micro organisms must be cultured in microtitre plates, glass tubes or shaking flasks, with or without deviators. Preferably, 100 ml shake flasks filled with 10%? Vclumen, of the required culture medium are used. The bottles should be agitated on a rotary shaker (25 mm amplitude) using a range of speeds of 100-300 rpm. Lost by evaporation can be reduced by maintaining a humid atmosphere; alternatively, a mathematical correction must be made to take into account the losses due to evaporation. If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be examined.

The medium is inoculated to an ODdoc of 0.5 - 1.5 using cells grown on agar plates, for example CM plates (10 g / 1 glucose, 2.5 g / 1 NaCl, 2 g / 1 urea, 10 g / 1 polypeptone, 5 g / 1 yeast extract, 5 g / 1 meat extract, 22 g / 1 NaCl, 2 g / 1 NaCl, 2 g / 1 urea, 10 g / 1 polypetone, 5 g / 1 yeast extract, 5 g / 1 meat extract, 22 g / 1 agar, pH 6.8 with 2M NaOH) that have been incubated at a temperature of 30 ° C. the inoculation of the media is achieved through the introduction of a saline suspension of C. gl utamicum cells from CM plates or the addition of a liquid preculture of this bacterium. Example 8 - In vitro analysis of the function of mutant proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of a given altered enzyme must be adapted to the specific activity of the wild type enzyme, which is within the ability of a person with ordinary knowledge in the art. General information on enzymes, as well as specific details on structure, kinetics, principles, methods, applications and examples to determine many enzymatic activities can be found, for example, in the following references: Dixon, M., and Webb,? .C., (1979) Encimes, Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Gree an: San Francisco; Price, N.C., Stevens, L. (1982) Fundamentals of Enzymology, Oxford Univ. Press: Oxford; Boyer, P.D., edition (1983) The? Nzymes, 3a. Academic Press edition: New York; Bisswanger, H., (1994)? Nzy kinetik, 2a. VCH edition: Weinheim (ISBN 3527300325); Bergmeyer, H.U., Berg eyer, J., Graßl, M., editions (1983-1986) Methods of Enzymatic Analysis, 3a. edition volume I-XII, Verlag Chemie: Weinheim; and Ullmann's Encyclopedia of Industrial Chemistry (1987) vol. A9, "? Nzymes." VCH: Weinheim, pages 352-363. The activity of proteins that bind with DNA can be measured by several well established methods such as DNA band shift assays (also known as gel delay assay). The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (according to the writing in Colmar, H. et al (1995) MBO J. 14: 3895-3904 and references cited there) . Reporter gene test systems are well known and established for applications in both prokaryotic and eukaryotic cells, employing enzymes such as beta-galactosidase, green fluorescent protein and several others. The determination of membrane-transport protein activity can be carried out in accordance with techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, pages 85-137; 199-234; and 270-322. Example 9: Impact analysis of Mutant Protein on the production of the desired product The effect of genetic modification on C. gl utamxcum on the production of a desired compound (such as for example an amino acid) can be evaluated by culturing the finite microorganism under suitable conditions (such as those described above) and analyzing the cell medium and / or component for an increased production of the desired product (i.e., an amino acid). Such analysis techniques are well known to a person with ordinary skill in the art and include spectroscopy, thin layer chromatography, various types of staining methods, enzymatic and microbiological methods as well as analytical chromatography such as high performance liquid chromatography. performance (see, for example, Ullman,? nciclopedia de Industrial Chemistry, volume A2, pages 89-90 and pages 443-613, VCH: Weinheim (1985); Fallón, A.? t al., (1987) "Applications of HPLC in Biochemistry "in: Laboratory Techniques in Biochemistry and Molecular Biology, volume 17; REM et al. (1993) Biotechnology, volume 3, chapter III: "Product recovery and purification", pages 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral J.M.S. (1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of Industrial Chemistry, volume B3, chapter II, pages 1-27, VCH: Weinheim; and Dechow, F.J. 1989) Separation and purification techniques in biotechnology, Noyes Publications). In addition to the measurement of the final fermentation product it is also possible to analyze other components of the metabolic pathways used for the production of the desired compound, such as intermediates and by-products, to determine the overall yield, production and / or production efficiency of the compound . Methods of analysis include measurements of nutrient levels in the medium (eg, sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gases produced during fermentation. Standard methods for these measurements are presented in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds. IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references mentioned there. Example 10: Purification of the desired product from culture C. gl utamxcum Recovery of the desired product from the cells of C. gl utamicum or supernatant of the culture described above can be effected by various methods well known in the art. If the desired product is not secreted from the cells, the cells can be harvested from the culture by low speed centrifugation, the cells can be used by standard techniques, such as mechanical strength or sonication. The cell debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is preserved for further purification of the desired compound. If the product is secreted from the cells of C. gl utamicum, then the cells are removed from the culture by low speed centrifugation, and the supernatant fraction is retained for further purification. The supernatant fraction from any of the purification methods is subjected to chromatography with a suitable resin, wherein the desired molecule is retained in a chromatography resin while many of the impurities in the sample are not, or where the impurities are retained by the resin while the sample is not. Such chromatography steps can be repeated as necessary, using the same chromatography resin or different chromatography resins. A person with ordinary knowledge in the art is enabled to select appropriate chromatography resins and in their most effective application for a particular molecule to be purified. The purified producer can be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is optimized. There is a wide range of purification methods known in the art and the foregoing method of purification is not limiting in any way. Such purification techniques are described, for example, in Bailey, J.E. & Ollis, D.F.

Biochemicl Engineering Fundamentals, McGraw-Hill: New York (1986). The impurity identity of the isolated compounds can be evaluated by standard techniques. These techniques include high performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such methods of analysis are presented in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27-32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Dictionary of Industrial Chemistry, (1996) volume A27, VCH: Weinheim, pages 89-90, pages 521-540, pages 540-547, pages 559-566, 575-581 and pages 581-587: Micha! , G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallón, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, volume 17. Example 11: Cloning of a gene of Corynebacterium glutamicum involved in resistance to lincomycin using a reporter gene approach A. Identification of the gene that encodes the protein ie LM? 3 The plasmid pSL130 was constructed by ligation of the promoter region of aceB (paceB) of C. glutamicum (Kim, HJ et al. (1997) J. Microbiol. Biotechnol. 7: 287-292) in the polylinker of the lac operon fusion vector pRS415, which has no promoter (Simón, R.W. et al. (1987) Gene 53: 85-96). The plasmid pSL145 was constructed by ligating the resulting paceB-lac region in the? cloning vector. coli pACYC184. ? coli DH5aF 'was transformed with pSL145 and the resulting strain was used as a host to screen a genomic library of C. glutamicum (in pSL109). Transformants were screened by agar culture containing 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). A white colony, containing DNA that influences the expression of lacZ, was selected for further analysis. This clone was found to contain a 4 kB fragment of the gene library. Subclones were constructed in pSL109 and a subclone that retained the white genotype in X-gal plates was identified. This subclone was found to contain a 2.6 kB BamHI-XhoI fragment. (plasmid SL149-5). The fragment was sequenced and identified as a gene encoding a membrane protein (LMRB gene). The 1442 nucleotides of the coding sequence of the LMRB gene encode a polypeptide of 481 amino acid residues with a high percentage of hydrophobic amino acids. A search of GenBank determined that the LMRB protein has a level of identity of 40% with the protein product of the Bacillus subtilis ImrB gene (Accession No. GenBank AL009126, TR? MBL P94422), as determined using a CLUSTAL analysis W (using standard parameters). The LMRN protein contains a sequence pattern: I58-APALGPTLSG-167 (SEQ ID NO: 301), which resembles the consensus motif of the multiple drug resistance protein known GXXXGPXXGG (SEQ ID NO: 302) (Paulsen, IT and Skurray, RA (1993) Gene 124: 1-11). Accordingly, the LMRB protein was classified as a drug resistance protein. B. In vivo analysis of the ImrB function The LMRB gene was overexpressed in C. glutamicum AS019E12 (Kim, H. J. et al. (1997) J. Microbiol. Biotechnol: 287-292) using the plasmid pSL149-5, described above. The disorganization of the LMRB gene was achieved by using the vector pSL18-lmrB. This vector was constructed in the following manner: an internal fragment of the LMRB gene was amplified by polymerase chain reaction under standard conditions using the primers 5'-CTCCAGGATTGCTCCGAAGG-3 '(S? Q ID NO: 303) and 5' -CACAGTGGTTGACCACTGGC-3 '(SEQ ID NO: 304). The resulting polymerase chain reaction product was treated with T7 DNA polymerase and T7 polynucleotide kinase, and was cloned in the Smal site of piásmidc pSL16 (Kim, Y. H. and H.-S. Lee (1996) J. Microbiol. Biotechnol., 6 315-320). The disorder of the LMRB gene in C. glutamicum AS019E12 was effected by conjugation, in accordance with the previously described (Schwarzer and Puhler (1991) Bio / Technology 9: 84-87). The cells of C. glutamicum transformed with pSL149-5 showed resistance similar to that observed in the case of non-transformed cells against erythromycin, penicillin G, tetracycline, chloramphenicol, spectinomycin, nalidixic acid, gentamicin, streptomycin, ethyl bromide, m- carbonyl cyanide chlorophenylhydrazone (CCCP), and sodium dodecylisulfate. Significant differences were observed, however in terms of the resistance to lincomycin of transformed and non-transformed cells. It was found that C. glutamicum cells that overexpress LRMB can grow in the presence of 20 microg / ml lincomycin. In contrast, cells that do not overexpress LMRB (or cells that carry an LMRB disorder) could not grow on agar medium that contained 5 microg / ml of lincomycin. This effect was clearly visible in liquid culture. Overexpression of LMRB caused an increased resistance nine times (compared to the wild type) against lincomycin and the LMRB disorder resulted in a decreased resistance (28% compared to the wild type) to this antibiotic.

Example 12: Analysis of the sequences of genes of the invention The comparison of sequences and determination of percentage of homology between two sequences are known techniques, and can be achieved using a mathematical algorithm, as for example the algorithm of Karlin and Altschul (1990) Proc. Nati Acad. Sci. USA 87: 2264-68, modified as Karlin and Altschul (1990) Proc. Nati Acad. Sci. USA 90: 5873-77. Said algorithm is incorporated in the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215: 403-10. Searches of nucleotides by BLAST can be carried out with the NBLAST program, result = 100, word length = 12 to obtain nucleotide sequences that are homologous with the SRT nucleic acid molecules of the invention. Protein searches with BLAST can be carried out with the NBLAST program, result = 50, word length = 3 to obtain amino acid sequences that are homologous with SRT protein molecules of the invention. To obtain spaced alignments for comparison purposes, Gapped BLAST can be used in accordance with that described in Altschul et al., (1997 Nucleic Acids Res. 25 (17): 3389-3402) When using the BLAST and Gapped BLAST programs, a person with ordinary knowledge in the field will know how to optimize the parameters of the program (for example XBLAST and NBLAST) for the specific sequence in the process of analysis.

Another example of a mathematical algorithm used for the comparison of sequences is the algorithm of Meyers and Milier (1988) Comput. Appl. Biosci. 4: 11-17). This algorithm is incorporated into the ALIGN program (version 2.0) that is part of the GCG sequence alignment programmatic package. When the ALIGN program is used to compare amino acid sequences, a weight residue table PAM120, a space length penalty of 12, and a space penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANC? and ADAM, described in Torelli and Robotti (1994) Comput.

Appl. Biosci. 10: 3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85: 2444-8). The percentage of homology between two amino acid sequences can also be achieved using the GAP program in the programmatic GCG package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a space weight of 12, 10, 8, 6 or 4 and a length weight of 2, 3, or 4. The percentage of homology between two nucleic acid sequences can be achieved by using the GAP program in the programmatic GCG package standard parameters, such as a space weight of 50 and a length weight of 3. A comparative analysis of the gene sequences of the invention with those present in GenBank was performed using known techniques (see, for example, Bexevanis and Oueilette , eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Protein, John Wiley and Sons: New York). The gene sequences of the present invention were compared with genes present in GenBank in a three-step process. In a first step, a BLASTN analysis (eg, a local alignment analysis) was carried out for each of the sequences of the invention against the nucleotide sequences present in GenBank, and the top 500 results were retained for further analysis . A subsequent FASTA search (for example, a combined alignment and global alignment analysis, where limited regions of the sequences are aligned) was performed on these 500 results. Each gene sequence of the present invention was subsequently globally aligned with each of the three superior results according to FASTA, using the GAP program in the programmatic GCG package (by using standard parameters). To obtain correct results, the length of the sequences extracted from GenBank were adjusted to the length of the search sequences by methods well known in the art. The results of this analysis are presented in Table 4. The resulting data are identical to the data that could have been obtained if a GAP (global) analysis was carried out only on each one of the genes of the invention in comparison with each one of them. the references in GenBank, but a significantly reduced computation time was required in comparison with this GAP analysis in the entire (global) database. Sequences of the present invention for which no alignments above the cut-off values were obtained are indicated in Table 4 by the absence of alignment information. A person with ordinary knowledge in the art will further understand that the homology percentages according to GAP presented in table 4 under the heading "GAP homology percentage" are listed in the European numerical form, where a "," represents a point decimal. For example, a value of "40,345" in this column represents "40,345". Example 13: Construction and Operation of DNA Micro Sets The sequences of the invention can be further used in the construction and application of DNA microsets (the design, methodology, and uses of DNA sets are well known in the art and are described, as for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al. (1997) Science 278: 680-686). Micro-sets of DNA are flexible solid supports consisting of nitrocellulose, nyion, glass, silicone, and other materials. Nucleic acid molecules can be joined on the surface in an orderly manner. After appropriate labeling, other nucleic acids or mixtures of nucleic acids can be hybridized onto the immobilized nucleic acid molecules and the label can be used to measure the individual signal strengths of the hybridized molecules in defined regions. This methodology allows the simultaneous quantification of the relative or absolute quantity of all nucleic acids or nucleic acids selected in the applied nucleic acid sample or mixture. The DNA microsets, therefore, allow an analysis of the expression of multiple (up to 6800 or more) nucleic acids in parallel (see, for example, Schena, M. (1996) BioEssays 18 (5): 427-431). The sequences of the invention can be used to design oligonucleotide primers that can amplify defined regions of one or more genes of C. gl utamicum by a nucleic acid amplification reaction such as polymerase chain reaction. The direction and design of the 5 'or 3' oligonucleotide primers or suitable linkers allows the covalent attachment of the resulting PCR products on the surface of a support medium as described above (also described, for example, Shena, M. et al., IJ 995) Science 270: 467-470). The nucleic acid microsets can also be constructed by oligonucleotide synthesis in situ in accordance with that described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367. Using photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups that are photolabile are activated in this way and subjected to addition of nucleotides, while regions masked in relation to light are not subject to any modification. Subsequent cycles of protection and activation with light allow the synthesis of different oligonuclectides in defined positions. Small defined regions of the genes of the invention can be synthesized in microassays by solid phase oligonucleotide synthesis. The nucleic acid molecules of the invention present in a mixture or mixture of nucleotides can be hybridized with the microsets. These nucleic acid molecules can be labeled according to standard methods. In summary, nucleic acid molecules (e.g., mRNA molecules or AD molecules) are labeled by incorporation of labeled nucleotides either isotopically or fluorescently, e.g., during reverse transcription or synthesis of AD ?. Hybridization of nucleic acids labeled with microsets is described (for example, in Schena, M. et al. (1995) supra, Wodicka, L. Et al. (1997), supra; and DeSaizieu A. et al. (1998) , supra). The detection and quantification of the hybridized molecule conform to the specific incorporated marker. Radioactive labels can be detected, for example, in accordance with that described in Schena, M. et al. (1995) supra) and fluorescent markers can be detected, for example, through the method of Salon et al. (1996) Genome Research 6: 639-645). The application of the sequences of the invention to DNA microarray technology, as described above, allows comparative analysis of different strains of C. gl utamxcum or other corynebacteria. For example, studies of variations between strains, based on profiles of individual transcripts and the identification of genes that are important for properties of specific and / or desired strains such as pathogenicity, productivity and tolerance to stress are facilitated by nucleic acid cluster methodologies . Likewise, comparisons of the expression profile of genes of the invention during the course of a fermentation reaction are possible using nucleic acid conjugate technology. Example 14: Analysis of the dynamic characteristics of cell protein populations (Preteomy) The genes, compositions and methods of the invention can be applied to the study of the interactions and dynamic characteristics of protein populations, which are known as "proteomics". Protein populations of interest include, but are not limited to, the total protein population of C. gl utamicum (eg, compared to the protein populations of other organisms), the proteins that are active under specific environmental or metabolic conditions ( for example, during fermentation, at high or low temperature, or at a high or low pH), or the proteins that are active during specific phases of growth and development. Protein populations can be analyzed by several well known techniques, such as for example gel electrophoresis. Cell proteins can be obtained, for example, by lysis or extraction, and can be separated from each other using various electrophoretic techniques. Electrophoresis in sodium dodecyl sulfate - polyacrylamide gel (SDS-PAGE) separates proteins to a large extent based on their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAG?) Separates proteins by their isoelectric point (reflecting not only the amino acid sequence but also post translational modifications of the protein.) Another, more preferred, protein analysis method is the consecutive combination of IEF-PAGE and SDS-PAG ?, which is known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998)? lectrophoresis 19 3217-3221; Fountoulakis et al. (1998)? Lectrophoresis 19 1193-1202; Lange et al. (1997)? Lectrophoresis 18 1184-1192; Antelmann et al. (1997)? Lectrophoresis 18 1451-1463). Other separation techniques can also be used for the separation of proteins, such as capillary gel electrophoresis; such techniques are well known. Separated proteins per these methods can be visualized by standard techniques, for example staining or marking. Suitable stains are known in the art and include Coomassie Brilliant Blue, silver stain, fluorescent dyes such as Sypro Ruby (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors (eg, 35S-methionine, jS-cysteine, amino acids labeled with 14C, 1 CN-amino acids, amino acids marked with 13C or ""? O3 or 15? HJ) in the C. glutamicum medium allows the labeling of proteins from these cells before their separation. Similarly, fluorescent labels can be used. These labeled proteins can be extracted, isolated and separated according to ccr. the techniques previously described. Proteins visualized by these techniques can be further analyzed by measuring the amount of stain or marker that is used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or other gels. Comparisons of proteins in gels can be carried out for example, by means of optical comparison, spectroscopy, image exploration and gel analysis, or through the use of photographic films and sieves. Such techniques are well known. To determine the identity of a given protein, direct sequencing or other standard techniques can be employed. For example, sequencing of N-terminal and / or C-terminal amino acids (eg, Edman degradation) can be employed, as well as mass spectrometry (in particular MALDI or ESI techniques (see, for example, Langen et al. (1997)). Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. gíutamicum proteins by these techniques. The information obtained by these methods can be used to compare patterns of protein presence, activity or modification between different samples of various biological conditions (eg, different organisms, different fermentation time points, different media conditions, or different biotopes, among other) . Data obtained from these experiments, alone or in combination with other techniques, can be used for several applications, for example to compare the behavior of several organisms in a given situation (eg, metabolic) to increase the productivity of strains that produce chemicals fine or to increase the efficiency of the production of fine chemicals. Equivalents Persons of ordinary skill in the art will recognize, or may determine by using only routine experiments, many equivalents of the specific embodiments of the invention described herein. Such equivalents are within the framework of the following claims.

LIST OF SEQUENCES < 110 > BASF Aktiengesellschaft < 120 > GENE OF CORYNEBACTERIUM GLUTAMICUM CODING TOLERANCE PROTEINS AND STRESS RESISTANCE < 130 > BGI-124CPPC < 140 > PCT / US00 / 00922 < 141 > 2000-06-23 < 150 > 60/141031 < 151 > 1999-06-25 < 150 > 60/142692 < 151 > 1999-07-01 < 150 > 60/151214 < 151 > 1999-08-27 < 150 > DE 19930429.7 < 151 > 1999-07-01 < 150 > DE 19931413.6 < 151 > 1999-07-08 < 150 > DE 19931457.8 < 151 > 1999-07-08 < 150 > DE 19931541.8 < 151 > 1999-07-08 < 150 > DE 19932209.0 < 151 > 1999-07-09 < 150 > DE 19932230.9 < 151 > 1999-07-09 < 150 > DE 19932914.1 < 151 > 1999-07-14 < 150 > DE 19940764.9 < 151 > 1999-08-27 < 150 > DE 19941382.7 < 151 > 1999-08-31 < 160 > 306 < 210 > 1 < 211 > 1566 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS | »¿¿^^^ < 222 > (101) .. (1543) < 223 > RXA01524 < 400 > 1 ttgtggcact ctttagtagt tttttctcat agctcagttt cgcaacttta gagaactcta 60 gaaactgagc ttcatgctgt gaaaggcctt ttctccattc atg_ gat tcc caa att 115 Met Asp Ser Gln He 1 5 aat act cag acc tct ccg gca gct gcg aag ctg ect agg gag gtc gtt 163 Asn Thr Gln Thr Ser Pro Ala Ala Wing Lys Leu Pro Arg Glu Val Val 10 15 20 gtt gtt ctt tcg atc ctc gtg gtt tcc gcg atg atc atg att ctt aat 211 Val Val Leu Ser He Leu Val Val Ser Wing Met He Met He Leu Asn 25 30 35 gaa acc att ctg tcg gtt gcg ttg ect aka atc atg gaa gat ttc aka 259 Glu Thr He Leu Ser Val Ala Leu Pro Ser He Met Glu Asp Phe Ser 40 45 50 gtg ect gaa act act gca cag tgg ttg acc act ggc ttt atg ttg acg 307 Val Pro Glu Thr Thr Ala Gln Trp Leu Thr Thr Gly Phe Met Leu Thr 55 60 65 atg gca gtg gtg att cca act act ggt tat ctg ctt gat cgt ttt tcc 355 Met Wing Val Val He Pro Thr Thr Gly Tyr Leu Leu Asp Arg Phe Ser 70 75 80 85 act aag acg atc ttt gtt act gcg ttg ttg ttc ttt acg gtt ggt acg 403 Thr Lys Thr He Phe Val Thr Ala Leu Leu Phe Phe Thr Val Gly Thr 90 95 100 ttg act gcg gcg ttg gct cca acg ttt gcg gtg ctg ctt ggt gct cgt 451 Leu Thr Ala Ala Leu Ala Pro Thr Phe Ala Val Leu Leu Gly Ala Arg 105 110 115 atc gtt cag gcg gtt ggt act gcg ctg gtg atg ect ttg ctg atg acg 499 He Val Gln Ala Val Gly Thr Ala Leu Val Met Pro Leu Leu Met Thr 120 125 130 gtt acg ttg acg gtg gtt ect gcg gag cgt cgt ggt tcg atg atg ggc 547 Val Thr Leu Thr Val Val Pro Ala Glu Arg Arg Gly Met Met Met Gly 135 140 145 att att tcc atc gtg att tct gtt gcg ccg gcg ctt ggt ect acg ttg 595 He He Ser I Am He Val He Ser Val Ala Pro Ala Leu Gly Pro Thr Leu 150 155 160 165 tct ggt gtc att ctt aac tct ttg acc tgg cac tgg ttg ttt tgg atg 643 Ser Gly Val He Leu Asn Ser Leu Thr Trp His Trp Leu Phe Trp Met 170 175 180 atg ctt ccg atc gtt gtt atc gct ttg gta att ggt ttc ttc ttg atc 691 Met Leu Pro He Val Val He Ala Leu Val He Gly Phe Phe Leu He 185 190 195 aaa aat atc ggc gaa acc aag atc acc cca ctg gat gtt ctg tct gtc 739 Lys Asn He Gly Glu Thr Lys He Thr Pro Leu Asp Val Leu Ser Val 200 205 210 atc ctt tcg gtg ttt gcc ttc ggt ggt ttg gtg tac ggc ttc agt tcc 787 He Leu Ser Val Phe Wing Phe Gly Gly Leu Val Tyr Gly Phe Ser Ser 215 220 225 ttc gga gca atc ctg gag ggc gaa ggc acc gta ggt atc ttc gcg atc 835 Phe Gly Ala He Leu Glu Gly Glu Gly Thr Val Gly He Phe Ala He 230 235 240 245 gtc gtt ggc gcc atc gca ctc ctc atc ttt gct ttg cga cag cac caa 883 Val Val Gly Ala He Ala Leu Leu He Phe Ala Leu Arg Gln His Gln 250 255 260 ctc ggc aag caa gac aaa gca ctg atg gat ctc cga gcc ttc aag gtg 931 Leu Gly Lys Gln Asp Lys Ala Leu Met Asp Leu Arg Wing Phe Lys Val 265 270 275 agg aac ttc age ttc aka ttg acc acc atc ctt ttg gcg ttc ggc gcg 979 Arg Asn Phe Ser Phe Ser Leu Thr Thr He Leu Leu Ala Phe Gly Ala 280 285 290 atg ctc gga acc gtc atg gtt ttg cca atc tac ctg cag act tcc ctc 1027 Met Leu Gly Thr Val Met Val Leu Pro He Tyr Leu Gln Thr Ser Leu 295 300 305 gga gtt act gct ttg gtg gtt gtt gt gtc gtc ctc 1075 Gly Val Thr Ala Leu Val Thr Gly Leu Val Val Met Pro Gly Gly Leu 310 315 320 325 ctc cag ggt ctg atc age cca ttc atc gga cgt tcc tac gac aag gtc 1123 Leu Gln Gly Leu He Ser Pro Phe He Gly Arg Phe Tyr Asp Lys Val 330 335 340 ggt cca cgc ctg ctg att ccc gga gca att gcg ctg gct atc gcg 1171 Gly Pro Arg Pro Leu Leu He Pro Gly Ala He Ala Leu Ala He Wing 345 350 355 gca tcc tcg atg act ttt ctc aat gag aat tea ccc gtg tgg atg gtc 1219 Wing Being Met Thr Phe Leu Asn Glu Asn Ser Pro Val Trp Met Val 360 365 370 gtg gtc atg cac gtt gtg ttc age atc ggc atg tgt ttg atg atg acc 1267 Val Val Met His Val Val Phe Ser He Gly Met Cys Leu Met Met Thr 375 380 385 ect ctc atg acc acc gct ctc ggc gcc ctt ccg aag cac ctc tat ggt 1315 Pro Leu Met Thr Thr Ala Leu Gly Ala Leu Pro Lys His Leu Tyr Gly 390 395 400 405 cac ggc tcc gca att ttg aac acg ttc caa cag cte gca ggc gca gcc 1363 His Gly Ser Ala He Leu Asn Thr Phe Gln Gln Leu Ala Gly Ala Wing 410 415 420 gga here gcg atc atg att gca gca ctt tcc t tc ggc act tcc att gca 1411 Gly Thr Wing He Met Wing Wing Wing Leu Ser Phe Gly Thr Ser Wing Wing 425 430 435 gcg tct tcg gga tct gcg cat gct gaa gct gtt scc gct ggt acc aag 1459 Wing Being Ser Gly Ser Wing His Wing Glu Wing Val Wing Wing Gly Thr Lys 440 445 450 gtt gcg ttc atc gca ggc gca atc atc gcg gtg atc gct ttg gtt gtt 1507 Val Ala Phe He Allah Ala Gly Ala He He Ala Ala He Ala Leu Val Val 455 460 465 tcc ctc ttc gtc act cgc gtc gag gaa gaa gct cac taaataccaa 1553 Ser Leu Phe Val Thr Arg Val Glu Glu Glu Ala His 470 475 480 aaaatggggc aga 1566 < 210 > 2 < 211 > 481 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 2 Met Asp Ser Gln He Asn Thr Gln Thr Ser Pro Wing Wing Wing Lys Leu 1 5 10 15 Pro Arg Glu Val Val Val Val Leu Ser He Le Val Val Ser Wing Met 20 25. 30 He Met He Leu Asn Glu Thr He Leu Ser Val Ala Leu Pro Ser He 35 40 45 Met Glu Asp Phe Ser Val Pro Glu Thr Thr Wing Gln Trp Leu Thr Thr 50 55 60 Gly Phe Met Leu Thr Met Wing Val Val He Pro Thr Thr Gly Tyr Leu 65 70 75 80 Leu Asp Arg Phe Ser Thr Lys Thr He Phe Val Thr Ala Leu Leu Phe 85 90 95 Phe Thr Val Gly Thr Leu Thr Ala Ala Leu Ala Pro Thr Phe Ala Val 100 105 110 Leu Leu Gly Wing Arg He Val Gln Wing Val Gly Thr Wing Leu Val Met 115 120 125 Pro Leu Leu Met Thr Val Thr Leu Thr Val Val Pro Wing Glu Arg Arg 130 135 140 Gly Ser Met Met Met Gly He He Ser He Val He Ser Val Val Ala Pro Wing 145 150 155 160 Leu Gly Pro Thr Leu Ser Gly Val Le Le Asn Ser Leu Thr Trp His 165 170 175 Trp Leu Phe Trp Met Met Leu Pro He Val Val He Ala Leu Val He 180 185 190 Gly Phe Phe Leu He Lys Asn He Gly Glu Thr Lys He Thr Pro Leu 195 200 205 Asp Val Leu Ser Val He Leu Ser Val Phe Wing Phe Gly Gly Leu Val 210 215 220 Tyr Gly Phe Ser Ser Phe Gly Wing He Leu Glu Gly Glu Gly Thr Val 225 230 235 . 240 Gly He Phe Wing He Val Val Gly Wing He Wing Leu Leu He Phe Wing 245 250 255 Leu Arg Gln His Gln Leu Gly Lys Gln Asp Lys Wing Leu Met Asp Leu 260 265 270 Arg Wing Phe Lys Val Arg Asn Phe Ser Phe Ser Leu Thr Thr He Leu 10 275 280 285 Leu Ala Phe Gly Ala Met Leu Gly Thr Val Met Val Leu Pro He Tyr 290 295 300 Leu Gln Thr Ser Leu Gly Val Thr Ala Leu Val Thr Gly Leu Val Val 305 310 315 320 Met Pro Gly Gly Leu Leu Gln Gly Leu He Ser Pro Phe He Gly Arg 325 330 335 Phe Tyr Asp Lys Val Gly Pro Arg Pro Leu Leu He Pro Gly Ala He 15 340 345 350 Wing Leu Wing Wing Wing Being Ser Met Thr Phe Leu Asn Glu Asn Ser 355 360 365 Pro Val Trp Met Val Val Val Met His Val Val Phe Ser He Gly Met 370 375 380 Cys Leu Met Met Thr Pro Leu Met Thr Thr Ala Leu Gly Ala Leu Pro 385 390 395 400 Lys His Leu Tyr Gly His Gly Be Wing He Leu Asn Thr Phe Gln Gln 20 405 410 415 Leu Wing Gly Wing Wing Gly Thr Wing He Met Wing Wing Wing Leu Ser Phe 420 425 430 Gly Thr Ser Wing Wing Wing Ser Gly Wing Wing His Wing Wing Wing Val 435 440 445 Wing Wing Gly Thr Lys Val Wing Phe He Wing Ala Gly Wing He He Wing Val 450 455 460 He Wing Leu Val Val Ser Leu Phe Val Thr Arg Val Glu Glu Glu Wing 25 465 470 475 480 - '- * M - His < 210 > 3 < 211 > 371 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (52) .. (348) < 223 > RXA00497 < 400 > 3 tggaaaccca caaccggcac acacaaaatt tttctcatgg agggattcac c gtg gca 57 Val Ala 1 aac gtc aac atc aag ccg ctt gag gac aag atc ctc gtt cag atc aac 105 Asn Val Asn He Lys Pro Leu Glu Asp Lys He Leu Val Gln He Asn 5 10 15 gaa gca gag acc action acc gct tcc ggc ctg gtc att cca gat tcc gct 153 Glu Wing Glu Thr Thr Thr Wing Ser Gly Leu Val He Pro Asp Ser Wing 20 25 30 aag gaa aag cca caa gag gca acc gtt atc gca gtt ggc cca ggc cgc 201 Lys Glu Lys Pro Gln Glu Wing Thr Val He Wing Val Gly Pro Gly Arg 35 40 45 50 ttc gat gac aag ggt aac cgc atc cca ctg gac atc aag gaa gat gac 249 Phe Asp Asp Lys Gly Asn Arg He Pro Leu Asp He Lys Glu Asp Asp 55 60 65 gtt gtg atc ttc tc cgt tac ggc ggc acc gag atc aag ttc ggt ggc 297 Val Val He Phe Ser Arg Tyr Gly Gly Thr Glu He Lys Phe Gly Gly 70 75 • _ 80 gtg gag tac ttg ctt ctc tcc gct cgt gac atc ctc gca atc gtc gag 345 Val Glu Tyr Leu Leu Leu Ser Ala Arg Asp He Leu Ala He Val Glu 85 90 95 aag taggggataa gttcatggca aag 371 Lys < 210 > 4 < 211 > 99 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 4 Val Wing Asn Val Asn He Lys Pro Leu Glu Asp Lys He Leu Val Gln 1 5 10 15 He Asn Glu Wing Glu Thr Thr Thr Wing Ser Gly Leu Val He Pro Asp 20 25 30 Wing Wing Lys Glu Lys Pro Gln Glu Wing Thr Val lié Ala Val Gly Pro 35 40 .- 45 Gly Arg Phe Asp Asp Lys Gly Asn Arg He Pro Leu Asp He Lys Glu 50 55 60 Asp Asp Val Val He Phe Ser Arg Tyr Gly Gly Thr Glu He Lys Phe 65 70 75 80 Gly Gly Val Glu Tyr Leu Leu Leu Ser Ala Arg Asp He Leu Ala He 85 90 95 Val Glu Lys 10 < 210 > 5 < 211 > 1737 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1714) < 223 > RXN00493 < 400 > 5 cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60 15 gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115 Met Ala Lys Leu He .1 5 gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163 Wing Phe Asp Gln Asp Wing Arg Glu Gly He Leu Arg Gly Val Asp Ala 10 15 20 ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211 Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val 25 30 35 20 gtt ctt gat aag gca ttc ggc gga ect ctg gtc acc aac gac ggt gtc 259 Val Leu Asp Lys Wing Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val 40 45 50 acc att gcc cgcp gac atc gac ctt gag gat ect ttt gag aac ctc ggt 307 Thr He Wing Arg Asp He Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly 55 60 65 gcg cag ctg gtg aag tcc gtt gct gag aag acc aac gac atc gct ggt 355 Wing Gln Leu Val Lys Ser Val Wing Val Lys Thr Asn Asp He Wing Gly 70 75 80 '85 25 j ^ gj ^ gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403 Asp Gly Thr Thr Thr Wing Thr Leu Leu Wing Gln Ala Leu He Wing Glu 90 95 100 ggc ctg cgc aac gtt gct gct ggc gcac cca atg gag ctc aac aag 451 Gly Leu Arg Asn Val Wing Wing Gly Wing Asn Pro Met Glu Leu Asn Lys 105 110 115 ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499 Gly He Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Glu Leu Lys Wing Arg 120 125 130 gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547 Wing Thr Glu Val Ser Asp Thr Lys Glu He Wing Asn Val Wing Thr Val 135 140 145 tea tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gcg ggg atg gaa 595 Ser ser arg Asp Glu Val Val Gly Glu He Val Wing Wing Wing Met Glu 150 155 160 '165 aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643 Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser He 170 175 - 180 gag act gct ctc gag gtc acc gaa ggt att tct tcc gac aag gcc tac 691 Glu Thr Ala Leu Glu Val Thr Glu Gly He Being Phe Asp Lys Gly Tyr 185 190 195 ctt tcc ect tat tcc atc aac gac aac gac act cag ggc gtc ctg 739 Leu Ser Pro Tyr Phe He Asn Asp Asn Thr Gln Gln Wing Val Leu 200 205 210 gac aac ect gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787 Asp Asn Pro Wing Val Leu Leu Val Arg Asn Lys He Ser Ser Leu Pro 215 220 225. gac ttc ctc ctc ctg gag aag gtt gtg gag tcc aac cgt ect ttg 835 Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu 230 235 240 245 ctg atc atc gca gaa gac gtc gag ggc gag ect ttg cag acc ctg gtt 883 Leu He He Wing Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val 250 255 260 gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tct ect 931 Val Asn Ser He Arg Lys Thr He Lys Val Val Wing Val Lys Ser Pro 265 270 275 tac tcc ggt gac cgc cgc aag gcg ttc atg gat gac ctg gct att gtc 979 Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala He Val 280 285 290 acc aag gca act gtg gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027 Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly He Asn Leu Asn Glu 295 300 305 gct ggc gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075 Wing Gly Glu Glu Val Phe Gly Thr Wing Arg Arg He Thr Val Ser Lys 310 315 320 325 gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123 Asp Glu Thr He Hep Val Asp Gly Wing Gly Ser Wing Glu Asp Val Glu 330 335 340 gca cgc cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171 Wing Arg Arg Gly Gln He Arg Arg Glu He Wing Asn Thr Asp Ser Thr 345 350 355 tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219 Trp Asp Arg Glu Lys Wing Glu Glu Arg Leu Wing Lys Leu Ser Gly Gly 360 365 370 att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267 He Wing Val He Arg Val Gly Wing Wing Thr Glu Thr Glu Val Asn Asp 375 380 385 cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315 Arg Lys Leu Arg Val Glu Asp Ala He Asn Ala Ala Arg Ala Ala Ala 390 395 400 405 caa gaa ggc gtt atc gct ggt ggc ggt tcc gct ttg gtt cag atc gct 1363 Gln Glu Gly Val He Wing Gly Gly Gly Wing Wing Leu Val Gln He Wing 410 415 r 420 gag act ctg aag gct tac gcc gaa gag ttc gaapggc gac cag aag gtc 1411 Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu * Gly Asp Gln Lys Val 425 430 - 435 ggc gtt cgc gca ctg gct act gct tg ggc aag cca gcg tac tgg atc 1459 Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys PJGO Wing Tyr Trp He 440 445 • '450 gcc tcc aac gca ggt ctt gac ggc tct gtt gtt gtt gca cgc act gct 1507 Wing Ser Asn Wing Gly Leu Asp Gly Ser Val Val Val Wing Arg Thr Wing 455 460 465 gc't ctg cca aac ggc gag ggc ttc aac gct gca act ttg gaa tac gga 1555 Wing Leu Pro Asn Gly Glu Gly Phe Asn Wing Wing Thr Leu Glu Tyr Gly 470 475 480. 485 aac ctg atc aac gac ggt gtc atc gac cca gtc aag gtc acc cat tcc 1603 Asn Leu He Asn Asp Gly Val He Asp Pro Val Lys Val Thr His Ser 490 495 500 gca gta gtg aat gca acc tct gtt gca cgc atg gtt ctg acc act gag 1651 Wing Val Val Asn Wing Thr Ser Val Wing Arg Met Val Leu Thr Thr Glu 505 510 515 gct tct gtt gtt gag aag ect gca gaa gaca gca gcc cat gca cat gca 1699 Wing Ser Val Val Glu Lys Pro Wing Glu Glu Ala Ala Asp Ala His Ala 520 525, 530 gga cat cat cac cac taaagttctg tgaaaaacac cgt 1737 Gly His His His His 535 < 210 > 6 < 211 > 538 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 6 Met Ala Lys Leu He Ala Phe Asp Gln Asp Ala Arg Glu Gly He Leu 1 5 10 15 Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro 20 25 30 Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val 35 40 45 Thr Asn Asp Gly Val Thr He Ala Arg Asp He Asp Leu Glu Asp Pro 50 55 .6.0 Phe Glu Asn Leu Gly Wing Gln Leu Val Lys Ser Val Wing Val Lys Thr 65 70 75 80 Asn Asp He Wing Gly Asp Gly Thr Thr Thr Wing Thr Leu Leu Wing Gln 85 90 95 Ala Leu He Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro 100 105 110 Met Glu Leu Asn Lys Gly He Ser Ala Ala Ala Glu Lys Thr Leu Glu 115 120 125 Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp. Thr Lys Glu He Wing 130 135 140 Asn Val Wing Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu He Val 145 150 155 160 Wing wing Wing Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu 165 170 175 Glu Ser Gln Ser He Glu Thr Wing Leu Glu Val Thr Glu Gly He Ser 180 185 190 Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe He Asn Asp Asn Asp Thr 195 200 205 Gln Gln Wing Val Leu Asp Asn Pro Wing Val Leu Leu Val Arg Asn Lys 210 215 220 He Be Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu 225 230 235 240 Being As Arg Pro Leu Leu He He Wing Glu Asp Val Glu Gly Glu Pro 245 250 255 Leu Gln Thr Leu Val Val Asn Ser He Arg Lys Thr He Lys Val Val 260 265 270 Wing Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Wing Phe Met Asp 275 280 285 Asp Leu Wing He Val Thr Lys Wing Thr Val Val Asp Pro Glu Val Gly 290 295 300 He Asn Leu Asn Glu Wing Gly Glu Glu Val Phe Gly Thr Wing Arg Arg 305 310 315 320 He Thr Val Ser Lys Asp Glu Thr He He Val Asp Gly Wing Gly Ser 325 330 335 Wing Glu Asp Val Glu Wing Arg Arg Gly Gln He Arg Arg Glu He Wing 340 345 350 Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Wing Glu Glu Arg Leu Wing 355 360 365 Lys Leu Ser Gly Gly He Wing Val He Arg Val Gly Ala Ala Thr Glu 370 375 380 Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala He Asn Ala 385 390 395 400 Ala Arg Ala Ala Ala Gln Glu Gly Val He Ala Gly Gly Gly Ser Ala 405 410 415 Leu Val Gln He Ala Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu 420 425 430 Gly Asp Gln Lys Val Gly Val Arg Ala Leu Ala T < hr Ala Leu Gly Lys 435 440 445 Pro Wing Tyr Trp Wing Wing Wing Asn Wing Gly Leu Asp Gly Wing Val Val 450 455 460 Val Wing Arg Thr Wing Wing Wing Leu Pro Asn Gly Glu Giy Phe Asn Wing Wing 465 470 475 480 Thr Leu Glu Tyr Gly Asn Leu He Asn Asp Gly Val He Asp Pro Val 485 490 495 Lys Val Thr His Ser Wing Val Val Asn Wing Thr Ser Val Wing Arg Met 500 505 510 Val Leu Thr Thr Glu Wing Ser Val Val Glu Lys Pro Wing Glu Glu Wing 515 520 525 Wing Asp Wing His Wing Gly His His His His 530 535 < 210 > 7 < 211 > 1339 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1339) < 223 > FRXA00498 < 400 > 7 cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60 gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115 Met Ala Lys Leu He 1 5 gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163 Ala Phe Asp Gln Asp Ala Arg Glu Gly He Leu Arg Gly Val Asp Wing 10 15 20 ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211 Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val 25 30 35 gtt ctt gat aag gca ttc ggc gga ect ctg gtc acc aac gac ggt gtc 259 Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val 40 45 50 acc att gcc cgc gac atc gac ctt gag gat ect ttt gag aac ctc ggt 307 Thr He Wing Arg Asp He Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly 55 60 65 gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355 Wing Gln Leu Val Lys Ser Val Wing Val Lys Thr Asn Asp He Wing Gly 70 75 80 85 gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403 Asp Gly Thr Thr Thr Wing Thr Leu Leu Wing Gln Ala Leu He Wing Glu 90 95 100 ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451 Gly Leu Arg Asn Val Ala Wing Gly Wing Asn Pro Met Glu Leu Asn Lys 105 110 115 ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499 Gly He Ser Wing Wing Wing Glu Lys Thr Leu Glu Glu Glu Leu Lys Wing Arg 120 125 130 gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547 Wing Thr Glu Val Ser Asp Thr Lys Glu He Wing Asn Val Wing Thr Val 135 140 145 tea tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595 Being Arg Asp Glu Val Val Gly Glu He Val Wing Wing Ala Met Glu 150 155 160 165 aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643 Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser He 170 175 180 gag act gct ctc gag gtc acc gaa ggt att tct tcc gac aag ggc tac 691 Glu Thr Ala Leu Glu Val Thr Glu Gly He Ser Phe Asp Lys Gly Tyr 185 190 '195 ctt tcc ect tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739 Leu Ser Pro Tyr Phe He Asn Asp Asn Asp Thr Gln Gln Wing Val Leu 200 205 210 gac aac ect gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787 Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys He Ser Ser Leu Pro 215 220 225 gac ttc ctc cca ctc ctc ctg gag aag gtt gtg gag tcc aac ccttct ttg 835 Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu 230 235 240 245 ctg atc atc gca gaa gac gtc gag ggc gag ect ttg cag acc ctg gtt 883 Leu He He Wing Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val 250 255 260 gtg aac tcc atc cgc aag acc atc aag gtc gtt g? a gtg aag tcc ect 931 Val Asn Ser He Arg Lys Thr He Lys Val Val Wing Val Lys Ser Pro 265 270 275 tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979 Tyr Phe Gly Asp Arg Arg Lys Wing Phe Met Asp Asp Leu Ala He Val 280 285 290 acc aag gca act gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027 Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly He Asn Leu Asn Glu 295 300 305 gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075 Wing Gly Glu Glu Val Phe Gly Thr Wing Arg Arg He Thr Val Ser Lys 310 315 320 325 gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123 Asp Glu Thr H e He Val Asp Gly Wing Gly Wing Wing Glu Asp Val Glu 330 335 340 gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc ac tc acc 1171 Wing Arg Arg Gly Gln He Arg Arg Glu He Wing Asn Thr Asp Ser Thr 345 350 355 tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219 Trp Asp Arg Glu Lys Wing Glu Glu Arg Leu Wing Lys Leu Ser Gly 360 365% 370 att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267 He Wing Val He Arg Val Gly Wing Wing Thr Glu Thr Glu Val Asn Asp 375 380 385 cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315 Arg Lys Leu Arg Val Glu Asp Ala He Asn Ala Ala Arg Ala Ala Ala 390 395 400 - 405 caa gaa ggc gtt atc gct ggt ggc 1339 Gln Glu Gly Val He Wing Gly Gly 410 < 210 > 8 < 211 > 413 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 8 Met Ala Lys Leu He Ala Phe Asp Gln Asp Ala Arg Glu Gly He Leu 1 5 10 15 Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro 20 25 30 Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val 35 40 45 Thr Asn Asp Gly Val Thr He Wing Arg Asp He Asp Leu Glu Asp Pro 50 55 60 Phe Glu Asn Leu Gly Wing Gln Leu Val Lys Ser Val Wing Val Lys Thr 65 70 75 80 Asn Asp He Wing Gly Asp Gly Thr Thr Thr Wing Thr Leu Leu Wing Gln 85 90 95 Wing Leu He Wing Glu Gly Leu Arg Asn Val Wing Wing. Gly Ala Asn Pro 100 105 110 Met Glu Leu Asn Lys Gly Be Ala Ala Ala Glu Lys Thr Leu Glu 115 120 125 Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu He Ala 130 135 140 Asn Val Ala Thr Val Be Ser Arg Asp Glu Val Val Gly Glu He Val 145 150 155 160 Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val 'Val Thr Val Glu 165 170 *; - 175 Glu Ser Gln Ser He Glu Thr Wing Leu Glu Val Thr Glu Gly He Ser 180 185 190 Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe He Asn Asp Asn Asp Thr 195 200 205 Gln Gln Wing Val Leu Asp Asn Pro Wing Val Leu Leu Val Arg Asn Lys 210 215 220 Be Ser Be Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu 225 230 235 -J 240 Ser Asn Arg Pro Leu Leu He He Wing Glu Asp Val Glu Gly Glu Pro 245 250 255 Leu Gln Thr Leu Val Val Asn Ser He Arg Lys Thr He Lys Val Val 260 265 270 Wing Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Wing Phe Met Asp 275 280 285 Asp Leu Wing He Val Thr Lys Wing Thr Val Val Asp Pro Glu Val Gly 290 295 300 He Asn Leu Asn Glu Wing Gly Glu Glu Val Phe Gly Thr Wing Arg Arg 305 310 315 320 He Thr Val Ser Lys Asp Glu Thr He He Val Asp Gly Wing Gly Ser 325 330 335 Wing Glu Asp Val Glu Wing Arg Arg Gly Gln He Arg Arg Glu He Wing 340 345 350 Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Wing Glu Glu Arg Leu Wing 355 360 • 365 Lys Leu Ser Gly Gly He Wing Val He Arg Val Gly Wing Wing Thr Glu 370 375 380 Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Wing He Asn Wing 385 390 395 400 Ala Arg Ala Ala Ala Gln Glu Gly Val He Ala Gly Gly 405 410 < 210 > 9 < 211 > 723 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (700) < 223 > RXA01217 < 400 > 9 gcgctgcctg cgaagactcg accaagacat tcgagtcggt cgcgggcatt ttttattttc 60 gcggccgagt gtccaccttc atccatgagg agaaatcact atg gca aag tac caa 115 Met Wing Lys Tyr Gln 1 5 acc att gag gct gct gtc cgc tct gag ttc ggc aag ggc tct gca cgt 163 Thr He Glu Ala Wing Val Arg Ser Glu Phe Gly Lys Gly Be Wing Arg 10 15 20 cgt gca cgc gtt gct ggc cag atc ect gct gtt gt tac ggc gca gat 211 Arg Wing Arg Val Wing Gly Gln He Pro Wing Val Val Tyr Gly Wing Asp 25 30 35 gtt gag tcc aac ctg cac gtc acc atc gac cac cgc acc ttc gcg gcg 259 Val Glu Ser Asn Leu His Val Thr He Asp His Arg Thr Phe Ala Wing 40 45 50 ctg gtt cgc cag gaa ggc gta aac gct gtt ctt gag ctc gac atc gag 307 Leu Val Arg Gln Glu Gly Val Asn Wing Val Leu Glu Leu Asp He Glu 55 60 65 ggc cag aag cag ctc acc atg atc aac cac atc gac cag aac gtg ctg 355 Gly Gln Lys Gln Leu Thr Met He Lys His He Asp Gln Asn Val Leu 70 75 80 85 acc ttc cac atc gac cac ttg gac ctg ctt gcc att aag cgc ggc gaa 403 Thr Phe His He Asp His Leu Asp Leu Leu Wing He Lys Arg Gly Glu 90 95 100 aag gtt gag gtt gac gtt cca gtt atc gtc gag ggc gag cca gct cca 451 Lys Val Glu Val Asp Val Pro Val He Val Glu Gly Pro Ala 105 105 115 ggc acc atg tgg gtt cag gat gct gac acc atc aag gtt gag gct gac 499 Gly Thr Met Trp Val Gln Asp Wing Asp Thr He Lys Val Glu Wing Asp 120 125 130 gtt ctg tcc atc ect gaa gag ttc acc gtt tcc atc gaa ggc ctt gag 547 Val Leu Ser He Pro Glu Glu Phe Thr Val Ser Glu Gly Leu Glu 135 140 145 ctc ggc gca cag atc acc gca gac atc aag ctc gag ggc gac acc 595 Leu Gly Wing Gln He Thr Wing Wing Asp He Lys Leu Glu Gly Asp Thr 150 155 160 • 165 acc ctg gtt gag gat ect gag acc ctc atc gtc aac atc gtt ctc cca 643 Thr Leu Val Glu Asp Pro Glu Thr Leu He Val Asn He Val Leu Pro 170 175 180 gct gag gaa gaa gac acc ga ga ga gac ga ga ga gaa gca 691 Wing Val Glu Glu Glu Asp Thr Glu Glu Asp Glu Wing Wing Glu Glu Wing 185 190, 195 gct act gag taagcttttt tagatagett tat 723Wing Thr Glu 200 < 210 > 10 < 211 > 200 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 10 Met Ala Lys Tyr Gln Thr He Glu Ala Ala Val Arg Ser Glu Phe Gly 1 5 10 15 Lys Gly Ser Ala Arg Arg Ala Arg Val Ala Gly Gln He Pro Ala Val 20 25 30 Val Tyr Gly Ala Asp Val Glu Ser Asn Leu His Val Thr He Asp His 35 40 45 Arg Thr Phe Ala Ala Leu Val Arg Gln Glu Gly Val Asn Ala Val Leu 50 55 60 Glu Leu Asp He Glu Gly Gln Lys Gln Leu Thr Met He Lys His He 65 70 75 80 Asp Gln Asn Val Leu Thr Phe His He Asp His Leu Asp Leu Leu Wing 85 90 95 He Lys Arg Gly Glu Lys Val Glu Val Asp Val Pro Val He Val Glu 100 105 110 Gly Glu Pro Wing Pro Gly Thr Met Trp Val Gln Asp Wing Asp Thr He 115 120 125 Lys Val Glu Wing Asp Val Leu Ser He Pro Glu Glu Phe Thr Val Ser 130 135 140 He Glu Gly Leu Glu Leu Gly Wing Gln He Thr Wing Wing Asp He Lys 145 150 155 160 Leu Glu Gly Asp Thr Thr Leu Val Glu Asp Pro Glu Thr Leu He Val 165 170 175 Asn He Val Leu Pro Ala Val Glu Glu Glu Asp Thr Glu Glu Asp Glu 180 185 190 Ala Ala Glu Glu Ala Ala Thr Glu 195 200 < 210 > 11 < 211 > 1603 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (33) .. (1580) < 223 > RXA00605 < 400 > 11 ataggtaacc ctcataaaag gaaggaatgc ta atg tct gag aag tea gca gca 53 Met Ser Glu Lys Ser Ala Ala 1 t 5 gac cag atc gta gat cgc gga atg cgt cca aag ctt tct gga aac act 101 Asp Gln He Val Asp Arg Gly Met Arg Pro Lys Leu Ser Gly Asn Thr 10 15 20 acc cgc cac aac gga gca cca gtt cca tct gag aac atc tcc gca acc 149 Thr Arg His Asn Gly Wing Pro Val Pro Ser Glu Asn He Ser Ala Thr 25 30 35 gca ggc cca cag ggt cca aac gtt ctc aat gac att cac ctc att gaa 197 Ala Gly Pro Gln Gly Pro Asn Val Leu Asn Asp He His Leu He Glu 40 45 50 55 aag ctc gca cac ttt aac cgc gag aac gtt cca gag cgt atc ect cac 245 Lys Leu Ala His Phe Asn Arg Glu Asn Val Pro Glu Arg He Pro His 60 65 70 gca aag ggc cac ggc gct ttc ggt gag ctg cac atc acc gag gac gta 293 Ala Lys Gly His Gly Ala Phe Gly Glu Leu His He Thr Glu Asp Val 75 80 85 tcc gaa tac acc aag gca gac ctg ttc cag ect ggt aag gtc acc ccg 341 Ser Glu Tyr Thr Lys Wing Asp Leu Phe Gln Pro Gly Lys Val Thr Pro 90 95 100 ctg gct gtt cgc ttc tct act gtt gct gct gag c ag ggc aka cca gat 389 Leu Ala Val Arg Phe Ser Thr Val Ala Gly Glu Gln Gly Ser Pro Asp 105 110 115 acc tgg cgc gac gtc cac ggc ttc gct ctt cgc ttc tac acc gaa gag 437 Thr Trp Arg Asp Val His Gly Phe Ala Leu Arg Phe Tyr Thr Glu Glu 120 125 130 135 ggc aac tac gac atc gtg ggt aac aac acc cca acc ttc ttc ctt cgt 485 Gly Asn Tyr Asp He Val Gly Asn Asn Thr Pro Thr Phe Phe Leu Arg 140 145 150 gac ggc atg aag tcc ccg gac ttc atc cac tea cag aag cgt ctc aac 533 Asp Gly Met Lys Phe Pro Asp Phe He His Ser Gln Lys Arg Leu Asn 155 1.60 165 aaag aac ggt ctg cgc gat gca gac atg cag tgg gat ttc tgg acc cgc 581 Lys Asn Gly Leu Arg Asp Ala Asp Met Gln Trp Asp Phe Trp Thr Arg 170 175 180 gca ect gaa tct gca cac cag gtg acc tac ctg atg ggt gac cgc ggt 629 Ala Pro Glu Ser Ala His Gln Val Thr Tyr Leu Met Gly Asp Arg Gly 185 190 195 acc ect aag acc aka cgc cac cag gac ggc ttc ggc aka cac acc ttc 677 Thr Pro Lys Thr Ser Arg His Gln Asp Gly Phe Gly Ser His Thr Phe 200 205 210 215 cag tgg atc aac gct gaa ggt aag cca gtt tgg gtt aag tac cac ttc 725 Gln Trp He Asn Wing Glu Gly Lys Pro Val Trp Val Lys Tyr His Phe 220 225 230 aag acc cgc cag ggc tgg gat tgc ttc acc gat gca gaa gca gca aag 773 Lys Thr Arg Gln Gly Trp Asp Cys Phe Thr Asp Ala Glu Ala Ala Lys 235 240 245 gtt gca ggc gag aac gct gac tac cag cgc gaa gac ctc tac aac gct 821 Val Wing Gly Glu Asn Wing Asp Tyr Gln Arg Glu Asp Leu Tyr Asn Wing 250 255 260 att gaa aac ggc gac tcc cca atc tgg gac gtc -ag gtt cag atc atg 869 He Glu Asn Gly Asp Phe Pro He Trp Asp Val ¿ys Val Gln He Met 265 270 275 ect ttc gag gat gca gag aac tac cgc tgg aac cea ttc gac ctg acc 917 Pro Phe Glu Asp Ala Glu Asn Tyr Arg Trp Asn Pro Phe Asp Leu Thr 280 285 290 295 aag acc tgg tcc cag aag gat tac cca ctg atc cca gtc ggt tac ttc 965 Lys Thr Trp Ser Gln Lys Asp Tyr Pro Leu He Pro Val Gly Tyr Phe 300 305 310 atc ctg aac cgc aac cca cgc aac ttc ttc gct cag atc gag cag ctt 1013 He Leu Asn Arg Asn Pro Arg Asn Phe Phe Wing Gln He Glu Gln Leu 315 320 325 gca ctg gat cca ggc aac atc gtt ect ggc gtc ggc ctg ccc cc gac 1061 Ala Leu Asp Pro Gly Asn He Val Pro Gly Val Gly Leu Ser Pro Asp 330 335 340 cgc atg ctc cag gca cgt atc tcc gca tac gct gac cag cag cgt tac 1109 Arg Met Leu Gln Wing Arg He Phe Wing Tyr Wing ASp Gln Gln Arg Tyr 345 350 355 cgc atc ggc gct aac tac cgc gac ctg cca gtg aac cgt cca atc aac 1157 Arg He Gly Wing Asn Tyr Arg Asp Leu Pro Val Asn Arg Pro He Asn 360 365 370 375 gag gtc aac acc tac age cgc gaa ggt tcc atg cag tac atc tcc gac 1205 Glu Val Asn Thr Tyr Ser Arg Glu Gly Ser Met Gln Tyr He Phe Asp 380 385 390 gct ggc gag ect tcc tac age ect aac cgc tac gac aag ggc gca 1253 Wing Glu Gly Glu Pro Ser Tyr Ser Pro Asn Arg Tyr Asp Lys Gly Wing 395 400 405 ggc tac ctg gat aac ggt acg g at tcc tcc tcc aac cac acc tcc tac 1301 Gly Tyr Leu Asp Asn Gly Thr Asp Being Ser Asn His Thr Ser Tyr 410 415 420 ggc cag gct gat gac atc tac gtc aac cca gac cca cac ggc acc gac 1349 Gly Gln Ala Asp Asp He Tyr Val Asn Pro Asp Pro His Gly Thr Asp 425 430 435 ctg gtt cgt gct gct tac gtc aag cac gat gat gac gac tcc atc 1397 Leu Val Arg Ala Wing Tyr Val Lys His Gln Asp Asp Asp Asp Phe He 440 445 450 '455 cag cca ggc atc cta tac cgc gag gtc ctg gat gag ggc gag aag gag 1445 Gln Pro Gly He Leu Tyr Arg Glu Val Leu Asp Glu Glu Glu Lys Glu 460 465 • 470 cga ttg gca gac aac atc tcc aac gca atg cag ggc atc tct gag gca 1493 Arg Leu Wing Asp Asn He Being Asn Wing Met Gln Gly He Ser Glu Wing 475 480 485 acc gag cca cgc gtc tac gac tac tgg aac aac gtt gat gag aac ctc 1541 Thr Glu Pro Arg Val Tyr Asp Tyr Trp Asn Asn Val Asp Glu Asn Leu 490 495. 500 ggc gct cgc gtc aag gag ctt tac ctc cag aag aag gct taagtccttc 1590 Gly Ala Arg Val Lys Glu Leu Tyr Leu Gln Lys Lys Ala 505 510 515 tgatttaaaa tga 1603 < 210 > 12 < 211 > 516 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 12 Met Ser Glu Lys Ser Ala Ala Asp Gln He Val Ásp Arg Gly Met Arg 1 5 10 15 Pro Lys Leu Ser Gly Asn Thr Thr Arg His Asn Gly Wing Pro Val Pro 20 25 30 Ser Glu Asn He Ser Ala Thr Ala Gly Pro Gln Gly Pro Asn Val Leu 35 40 45 Asn Asp He His Leu He Glu Lys Leu Wing His Phe Asn Arg Glu Asn 50 55 60 Val Pro Glu Arg He Pro His Wing Lys Gly His Gly Wing Phe Gly Glu 65 70 75 80 Leu His He Thr Glu Asp Val Ser Glu Tyr Thr Lys Wing Asp Leu Phe 85 90 95 Gln Pro Gly Lys Val Thr Pro Leu Wing Val Arg Phe Ser Thr Val Wing 100 105 110 Gly Glu Gln Gly Ser Pro Asp Thr Trp Arg Asp Val His Gly Phe Wing 115 120 4 125 Leu Arg Phe Tyr Thr Glu Glu Gly Asn Tyr Asp He Val Gly Asn Asn 130 135 140 Thr Pro Thr Phe Phe Leu Arg Asp Gly Met Lys Phe Pro Asp Phe He 145 150 155 160 His Ser Gln Lys Arg Leu Asn Lys Asn Gly Leu Aírg Asp Wing Asp Met 165 170 175 Gln Trp Asp Phe Trp Thr Arg Wing Pro Glu Ser Wing His Gln Val Thr 180 185 190 Tyr Leu Met Gly Asp Arg Gly Thr Pro Lys Thr Ser Arg His Gln Asp 195 200 205 Gly Phe Gly Ser His Thr Phe Gln Trp He Asn Wing Glu Gly Lys Pro 210 215 220 Val Trp Val Lys Tyr His Phe Lys Thr Arg Gln Gly Trp Asp Cys Phe 225 230 235 240 Thr Asp Wing Glu Wing Wing Lys Val Wing Gly Glu Asn Wing Asp Tyr Gln 245 250 255 Arg Glu Asp Leu Tyr Asn Wing He Glu Asn Gly Asp Phe Pro He Trp • 260 265 270 Asp Val Lys Val Gln Met Met Pro Phe Glu Asp Wing Glu Asn Tyr Arg 275 280 285 Trp Asn Pro Phe Asp Leu Thr Lys Thr Trp Ser Gln Lys Asp Tyr Pro 290 295 300 Leu He Pro Val Gly Tyr Phe He Leu Asn Arg Asn Pro Arg Asn Phe 305 310 315 320 10 Phe Wing Gln He Glu Gln Leu Wing Leu Asp Pro Gly Asn He Val Pro 325 330 335 Gly Val Gly Leu Ser Pro Asp Arg Met Leu Gln Wing Arg He Phe Wing 340 345 350 Tyr Wing Asp Gln Gln Arg Tyr Arg He Gly Wing Asn Tyr Arg Asp Leu 355 360 365 Pro Val Asn Arg Pro He Asn Glu Val Asn Thr Tyr Ser Arg Glu Gly 370 375 380 15 Ser Met Gln Tyr He Phe Asp Wing Glu Gly Glu Pro Ser Tyr Ser Pro 385 390 395 400 Asn Arg Tyr Asp Lys Gly Wing Gly Tyr Leu Asp Asn Gly Thr Asp Ser 405 410 415 Ser Ser Asn His Thr Ser Tyr Gly Gln Wing Asp Asp He Tyr Val Asn 420 425 430 Pro Asp Pro His Gly Thr Asp Leu Val Arg Wing Ala Tyr Val Lys His 435 440 445 20 Gln Asp Asp Asp Asp Phe He Gln Pro Gly He Leu Tyr Arg Glu Val 450 455 460 Leu Asp Glu Glu Glu Lys Glu Arg Leu Wing Asp Asn He Ser Asn Wing 465 470 475 480 Met Gln Gly He Ser Glu Wing Thr Glu Pro Arg Val Tyr Asp Tyr Trp 485 • 490 495 Asn Asn Val Asp Glu Asn Leu Gly Wing Arg Val Lys Glu Leu Tyr Leu 500 505 510 25 Gln Lys Lys Wing 515 < 210 > 13 < 211 > 2439 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (2416) < 223 > RXA00404 < 400 > 13 aagatccgat catcggcata cagaaacacc catctggccg aactttcctt tttctgcatg 60 catttctgca cacagtttct gcccgctgtt tctacgcata gtg gct ttg aaa cga 115 Val Ala Leu Lys Arg 1 5 ccc gaa gag aaa here gta aag atc gtg acc ata aaa cag act gac aac 163 Pro Glu Glu Lys Thr Val Lys He Val Thr He Lys Gln Thr Asp Asn 10 15 20 ate aat gac gat gat gtg tac age aac gct act gac ctt cca gta 211 He Asn Asp Asp Asp Leu Val Tyr Ser Asn Ala Thr Asp Leu Pro Val 25 30 35 ggc gtg aag aag tcc ect aaa atg tea ccg acc gcc cgc gtt ggt ctc 259 Gly Val Lys Lys Ser Pro Lys Met Ser Pro Thr Wing Arg Val Gly Leu 40 45 50 ctt gtc ttt ggg gtt atc gcg gcg ggt ggg tgg gga gca atc gct ttc 307 Leu Val Phe Gly Val He Wing Wing Val Gly Trp Gly Wing He Wing Phe 55 60 65 tcc cgt ggc gaa here atc aac tct gtg tgg ctg gtt ttg gcg gca gtt 355 Ser Arg Gly Glu Thr He Asn Ser Val Trp Leu Val Leu Ala Wing Val 70 75 80 85 ggt tcc tat atc att gcg ttt tct ttc tat gcc cga ctg att gaa tac 403 Gly Ser Tyr He He Wing Phe Ser Phe Tyr Wing Arg Leu He Glu T yr 90 95 100 aaa gtt gtt aag ccg aaa gat cag cga gca acc ccg gcg gaa tac gtt 451 Lys Val Val Lys Pro Lys Asp Gln Arg Ala Thr Pro Ala Glu Tyr Val 105 110 115 aat gac ggc aag gac tat gtc cca acg gat cgt cgt gtg ctt ttt ggc 499 Asn Asp Gly Lys Asp Tyr Val Pro Thr Asp Arg Arg Val Leu Phe Gly 120 125 130 cac cac ttt gca gct att gca ggt gcg cca ttg gtt gga ect gtc 547 His His Phe Ala Ala He Ala Gly Wing Gly Pro Leu Val Gly Pro Val 135 140 145 atc gcc gcg cag atg ggc tac ctg cca ggc acc ttg tgg att atc ctc 595 Met Wing Wing Gln Met Gly Tyr Leu Pro Gly Thr Leu Trp He He Leu 150 155 160 165 ggt gtg att ttc gcc ggt gca gtg cag gac tac cta gtg ctg tgg gtg 643 Gly Val He Phe Wing Gly Wing Val Gln Asp Tyr Leu Val Leu Trp Val 170 175 180 tct act cgt agg cgt gga cgc tea ctt ggc cag atg gtt cgt gat gaa 691 Ser Thr Arg Arg Arg Gly Arg Ser Leu Gly Gln Met Val Arg Asp Glu 185 190 195 atg ggc acg gtc ggt ggc gct gcc ggt atc ttg gcg acc atc tcc atc 739 Met Gly Thr Val Gly Gly Ala Wing Gly He Leu Ala Thr He Ser He 200 205 210 atc atc att atc gcg gtg ctc gca ttg atc gtg gtt aat gca ctg 787 Met He He He He Wing Wing Val Leu Wing Leu He Val Val Asn Wing Leu 215 220 225 gct gat tea cca tgg ggc gtt tcc tcc atc acc atg acc atc cca att 835 Wing Asp Ser Pro Trp Gly Val Phe Ser He Thr Met Thr He Pro He 230 235 240 245 gca ctg ttc atg ggt gtg tac ttg cgt tac ctg cgc cca ggt cgt gtt 883 Wing Leu Phe Met Gly Val Tyr Leu Arg Tyr Leu Arg Pro Gly Arg Val 250 255 260 act gaa gtg tcc atc atc ggt gtg gca ctg ctc ctg ctg gct atc gtt 931 Thr Glu Val Ser He He Gly Val Ala Leu Leu Leu Ala He Val 265 270 275 gct ggt ggt ggg ggg tgg gtt gc gac acc tea tgg ggc gtg gaa tgg ttc acc 979 Wing Gly Gly Trp Val Wing Asp Thr Ser Trp Gly Val Glu Trp Phe Thr 280 285 290 tgg tct aact ac ttg gcg ttg gcc ttg gt ttg tcg tac gga atc atg 1027 Trp Ser Lys Thr Thr Leu Wing Leu Wing Leu He Gly Tyr Gly He Met 295 300 305 gct gcg att ttg ccg gtg tgg ctg ctg ctt gca ccg cgc gat tac ctg 1075 Ala Ala He Leu Pro Val Trp Leu Leu Leu Ala Pro Arg Asp Tyr Leu 310 315 320 325 tct acc ttt atg aag atc ggc gtc atc ggt ctg ttg gca gtg ggt att 1123 Being Thr Phe Met Lys He Gly Val He Gly Leu Leu Wing Val Gly He 330 335 340 ttg ttc gca cgt ect gag gtg cag atg ect tcc gtg acc tcc tcc gca 1171 Leu Phe Wing Arg Pro Glu Val Gln Met Pro Ser Val Thr Ser Phe Wing 345 350 355 ctt gag ggc aac ggt ccg gtg ttc tct tgc agt ctg ttc cca ttc ctg 1219 Leu Glu Gly Asn Gly Pro Val Phe Ser Gly Ser L'eu Phe Pro Phe Leu 360 365 370 ttc atc acg att gcc tgt ggt gca ctg tct ggt ttc cac gca ctg att 1267 Phe He Thr He Ala Cys Gly Ala Leu Ser Gly Phe His Ala Leu He 375 380 3"85 tct tea gga acc acca cca aag ctt gtg gag aag gaa tcc cag atg cgc 1315 Ser Ser Gly Thr Thr Pro Lys Leu Val Glu Lys Glu Ser Gln Met Arg 390 395 400 405 atg ctc ggc tac ggc ggc atg ttg atg gaa tct ttc gtg gcg atg atg 1363 Met Leu Gly Tyr Gly Gly Met Leu Met Glu Ser Phe Val Wing Met Met 410 415 420 gca ctg atc acc gct att ctg gat cgt cac ctg tac tc tc atg 1411 Ala Leu He Thr Ala Val He Leu Asp Arg His Leu Tyr Phe Ser Met 425 430 435 aac gct ccg ctg gca ctg act ggt gga gat cca gca acc gca gct gag 1459 Asn Ala Pro Leu Ala Leu Thr Gly Gly Asp Pro Ala Thr Ala Ala Glu 440 445 450 tgg gtt aac tcc att ggg ctg here ggt gcg gat atc acc ccg gaa cag 1507 Trp Val Asn Ser He Gly Leu Thr Gly Wing Asp He Thr Pro Glu Gln 455 460 465 ctg tcg gaa gct gct gaa agt gtc gga gaa 'tcc act gtt att tcc cgt 1555 Leu Ser Glu Wing Wing Glu Ser Val Gly Glu Ser Thr Val He Ser Arg 470 475 480 485 ggc ggc gca gca cca acc ttg gcg ttc ggt atg tct gaa atc ctc tcc 1603 Thr Gly Gly Ala Pro Thr Leu Ala Phe Gly Met Ser Glu He Leu Ser 490 495 • 500 gga tcc atc ggc ggc ggc gct gga atg aag gcg ttc tgg tac cac tcc gcc 1651 Gly Phe He Gly Gly Wing Gly Met Lys Wing Phe Trp Tyr His Phe Wing 505 510: 515 atc atg ttt gag gct ctg ttc atc ctc act act gtg gat gca ggt act 1699 He Met Phe Glu Wing Leu Phe He Leu Thr Thr Val Asp Wing Gly Thr 520 525 '530 cgt gtg gct cgc ttt atg atg acc gat acc ttg ggc aat gtt cca ggt 1747 Arg Val Wing Arg Phe Met Met Thr Asp Thr Leu Gly Asn Val Pro Gly 535 540 545 ctg cgc cgt ttc aag gat ect tea tgg act ggt aac tgg att tct 1795 Leu Arg Arg Phe Lys Asp Pro Ser Trp Thr Val Gly TO sn Trp He Ser 550 555 560"'_ 565 acc gtg ttt gtg tgt gct cta tgg ggt gct att ttg ctc atg ggt gtt 1843 Thr Val Phe Val Cys Ala Leu Trp Gly Ala He Leu Leu Met Gly Val 570 575 580 acc gat cca ctg ggc ggc atc aac gtg ctt ttc cca cta ct tc ggt atc 1891 Thr Asp Pro Leu Gly Gly He Asn Val Leu Phe Pro Leu Phe Gly He 585 590 595 gct aac cag ctg ctc gcc gct att gca ctt gct ctc gtg ctg gtt gtt 1939 Wing Asn Gln Leu Leu Ala Wing Wing Wing Leu Wing Leu Val Leu Val Val 600 605 610 gtg gtg aag aag ggc ctg tac aag tgg gcg tgg att cca gct gtt ect 1987 Val Val Lys Lys Gly Leu Tyr Lys Trp Wing Trp He- Pro Wing Val Pro 615 620 625 ttg gca tgg gat ctc att gtc acg atg act gcg toa tgg cag aag att 2035 Leu Wing Trp Asp Leu He Val Thr Met Thr Wing Ser Trp Gln Lys He 630 635 640 645 ttc cac tct gat ccg gct att ggc tac tgg gct cag aac gcg aac ttc 2083 Phe His Ser Asp Pro Wing He Gly Tyr Trp Wing Gln Asn Wing Asn Phe 650 655 660 cgc gat gca aag tct caa ggc ctt acc gaa ttt ggt gcc gct aaa tct 2131 Arg Asp Ala Lys Ser Gln Gly Leu Thr Glu Phe Gly Wing Wing Lys Ser 665 670 675 ect gag gca atc gat gcg gtt atc cga aac acc atg att cag ggc atc 2179 Pro Glu Ala He Asp Ala Val He Arg Asn .Thr Met He Gln Gly He 680 685 690 ttg tcc atc ctg ttc gcg gtg ctc gtc ctc gtt gtt gtc ggc gc gcc 2227 leu ser he leu phe wing val leu val leu val val gly wing wing 695 700 705 att gcg gtg tgc atc aag tcc atc agg gct cgt gca gcc gga here ect 2275 He Wing Val Cys He Lys Ser He Arg Wing Arg Wing Wing Gly Thr Pro 710 715 720 725 ttg gag acc ga ga gact ect gat gact tct gag ttc tcc gcc cca 2323 Leu Glu Thr Thr Glu Glu Pro Asp Thr Glu Ser Glu Phe Phe Wing Pro 730 735 740 act gga ttc ctt gca tct tcc agg gat aag gaa gtc cag gcc atg tgg 2371 Thr Gly Phe Leu Wing Ser Ser Arg Asp Lys Glu Val Gln Wing Met Trp 745 750 755 gac gag cgc tac cca ggc ggt gcg ccc gtg tct tct gga ggg cac 2416 Asp Glu Arg Tyr Pro Gly Gly Wing Pro Val Ser Gly Gly His 760 765 770 taaaacatga tggctcttac tea 2439 < 210 > 14 < 211 > 772 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 14 Val Ala Leu Lys Arg Pro Glu Glu Lys Thr Val Lys He Val Thr He 1 5 10 15 Lys Gln Thr Asp Asn He Asn Asp Asp Asp Leu Val Tyr Ser Asn Wing 20 25 30 Thr Asp Leu Pro Val Gly Val Lys Lys Ser Pro Lys Met Ser Pro Thr 35 40 45 Wing Arg Val Gly Leu Leu Val Phe Gly Val He Wing Wing Val Gly Trp 50 55 60 Gly Wing He Wing Phe Ser Arg Gly Glu Thr He Asn Ser Val Trp Leu 65 70 75 80 Val Leu Ala Ala Val Gly Ser Tyr He He Ala Phe Ser Phe Tyr Ala 85 90 95 Arg Leu He Glu Tyr Lys Val Val Lys Pro Lys Asp Gln Arg Ala Thr 100 105 110 Pro Wing Glu Tyr Val Asn Asp Gly Lys Asp Tyr Val Pro Thr Asp Arg 115 120 125 Arg Val Leu Phe Gly His His Phe Wing Wing He Wing Gly Ala Gly Pro 130 135 140 Leu Val Gly Pro Val Met Ala Ala Gln Met Gly Tyf Leu Pro Gly Thr 145 150 '155' 160 Leu Trp He He Leu Gly Val He Phe Wing Gly Wing Val Gln Asp Tyr 165 170 175 Leu Val Leu Trp Val Ser Thr Arg Arg Arg Gly Arg Ser Leu Gly Gln 180 185 190 Met Val Arg Asp Glu Met Gly Thr Val Gly Gly Ala Wing Gly He Leu 195 200 205 Wing Thr He Ser He Met He He He He He Wing Ala Val Leu Ala Leu He 210 215 220 Val Val Asn Ala Leu Ala Asp Ser Pro Trp Gly Val Phe Ser He Thr 225 230 235 240 Met Thr He Pro He Wing Leu Phe Met Gly Val Tyr Leu Arg Tyr Leu 245 250 255 Arg Pro Gly Arg Val Thr Glu Val Ser He He Gly Val Wing Leu Leu 260 265 270 Leu Leu Wing He Val Wing Gly Gly Trp Val Wing Asp Thr Ser Trp Gly 275 280 285 Val Glu Trp Phe Thr Trp Ser Lys Thr Thr Leu Ala Leu Ala Leu He 290 295 300 Gly Tyr Gly He Met Ala Ala He Leu Pro Val Trp Leu Leu Leu Ala 305 310 315 320 Pro Arg Asp Tyr Leu Be Thr Phe Met Lys He Gly Val He Gly Leu 325 330 335 Leu Wing Val Gly He Leu Phe Wing Arg Pro Glu Val Gln Met Pro Ser 340 345 350 Val Thr Ser Phe Ala Leu Glu Gly Asn Gly Pro Val Phe Ser Gly Ser 355 360 365 Leu Phe Pro Phe Leu Phe He Thr He Wing Cys Gly Wing Leu Ser Gly 370 375 380 Phe His Wing Leu He Ser Ser Gly Thr Thr Pro Lys Leu Val Glu Lys 385 390 395 400 Glu Ser Gln Met Arg Met Leu Gly Tyr Gly Gly Met Leu Met Glu Ser 405 410 415 Phe Val Wing Met Met Wing Leu He Thr Wing Val He Leu Asp Arg His 420 425 '. 430 Leu Tyr Phe Ser Met Asn Wing Pro Leu Wing Leu Thr Gly Asp Pro 435 440 445 Wing Thr Wing Wing Glu Trp Val Asn Ser He Gly Leu Thr Gly Wing Asp 450 455 460 He Thr Pro Glu Gln Leu Ser Glu Wing Wing Glu Ser Val Gly Glu Ser 465 470 475 480 Thr Val He Be Arg Thr Gly Gly Wing Pro Thr Leu Wing Phe Gly Met 485 490 495 Ser Glu He Leu Ser Gly Phe He Gly Gly Wing Gly Met Lys Wing Phe 500 505 510 Trp Tyr His Phe Wing He Met Phe Glu Wing Leu Phe He Leu Thr Thr 515 520, 525 Val Asp Wing Gly Thr Arg Val Wing Arg Phe Met Met Thr Asp Thr Leu 530 535 540 Gly Asn Val Pro Gly Leu Arg Arg Phe Lys Asp Pro Ser Trp Thr Val 545 550 555 560 Gly Asn Trp Be Ser Thr Val Phe Val Cys Wing Leu Trp Gly Wing He 565 570 575 Leu Leu Met Gly Val Thr Asp Pro Leu Gly Gly He Asn Val Leu Phe 580 585 590 Pro Leu Phe Gly He Wing Asn Gln Leu Leu Wing Ala He Ala Ala Leu 595 600 605 Leu Val Leu Val Val Val Lys Lys Gly Leu Tyr Lys Trp Wing Trp 610 615 620 He Pro Wing Val Pro Leu Wing Trp Asp Leu He Val Thr Met Thr Wing 625 630 635 640 Ser Trp Gln Lys He Phe His Ser Asp Pro Wing He Gly Tyr Trp Wing 645 650 655 Gln Asn Wing Asn Phe Arg Asp Wing Lys Ser Gln Gly Leu Thr Glu Phe 660 665 670 Gly Wing Wing Lys Ser Pro Glu Wing He Asp Wing Val He Arg Asn Thr 675 680 685 Met He Gln Gly He Leu Ser He Leu Phe Wing Val Leu Val Leu Val 690 695 700 Val Val Gly Wing Wing He Wing Val Cys He Lys Ser He Arg Wing Arg 705 710 715 _ 720 Ala Ala Gly Thr Pro Leu Glu Thr Thr Glu Glu Pro Asp Thr Glu Ser 725 730 735 Glu Phe Phe Wing Pro Thr Gly Phe Leu Wing Ser Ser Arg Asp Lys Glu 740 745. 750 Val Gln Ala Met Trp Asp Glu Arg Tyr Pro Gly Gl Ala Pro Val Ser 755 760 765 Ser Gly His 770 < 210 > 15 < 211 > 255 < 212 > DNA < 213 > Corynebacterium glutamicum <; 220 > < 221 > CDS < 222 > (101) .. (232) < 223 > RXN03H9 < 400 > 15 tgggaggtgt cgcaccaagt acttttgcga agcgccatct gacggatttt caaaagatgt 60 atatgctcgg tgcggaaacc tacgaaagga ttttttaccc atg gct tata gaa 115 Met Wing Val Tyr Glu 1 5 ctc cca gac ctc gac tac gca tac gac gct ctc gag cca cac atc gtc 163 Leu Pro Glu Leu Asp Tyr Ala Tyr Asp Ala Leu Glu Pro His He Val 10 15 '20 gct gaa atc gag ctt gac cag tcc aag gac cac gca acc tac gtt 211 Wing Glu He Met Glu Leu Asp Gln Ser Lys Asp His Wing Thr Tyr Val 25 30 35 gcg ggc gca aat gca gca ctc taggcactag agaaggcacg cga 255 Ala Gly Ala Asn Ala Ala Leu 40 /, < 210 > 16 < 211 > 44 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 16 Met Wing Val Tyr Glu Leu Pro Glu Leu Asp Tyr Wing Tyr Asp Wing Leu 1 5 10 15 Glu Pro His He Val Wing Glu He Met Glu Leu Asp Gln Ser Lys Asp 20 25 30 His Wing Thr Tyr Val Wing Wing Wing Wing Ala Leu 35 40 < 210 > 17 < 211 > 249 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (226) < 223 > RXN03120 < 400 > 17 ctgggcagtt cttgggtacg accacatatc cggtcgcctg gttatcgagc agctcaccga 60 ccaggagggc aacatctcct tcgacatcac cccagttctg atg ctc gat atg tgg 115 Met Leu Asp Met Trp 1 5 gag cac gct ttc tac ctg cag tac atg aac gtt aag gca gat tac gtc 163 Glu His Wing Phe Tyr Leu Gln Tyr Met Asn Val Lys Wing Asp Tyr Val 10 15 20 aag gct gtt tgg aac gtc ttc aac tgg gac gac gca aga gca cgc ttc 211 Lys Wing Val Trp Asn Val Phe Asn Trp Asp Asp Ala Arg Ala Arg Phe 25 30 - 35 gca gca gct tcc aag taagcatttt tagtccgtgc aat 249 Ala Ala Ala Ser Lys 40 < 210 > 18 < 211 > 42 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 18 Met Leu Asp Met Trp Glu His Wing Phe Tyr Leu Gl Tyr Met Asn Val 1 5 10 15 Lys Wing Asp Tyr Val Lys Wing Val Trp Asn Val Phe Asn Trp Asp Asp 20 25 30 Wing Arg Wing Arg Phe Wing Wing Wing Lys 35 40 < 210 > 19 < 211 > 660 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (637) < 223 > RXN00575 < 400 > 19 gaagcgtccg caggcaagaa aacgtcccgg aaaacggcga taggggtcac ccgcgcatgt 60 ccggttgccg atctatttaa ataccaggac aattgcgtgc atg gtt gaa a gac gac 115 Met Val Glu Arg Asp 5 ttc act atc cga cca ccc gag ggt gat tcc ect cag gtg agg gac 163 Phe Thr He Arg Pro He Arg Glu Gly Asp Phe Pro Gln Val Arg Asp 10 15 20 atc tac gaa ttg ggc ctg gag acg gga cat gcg act tat gag act tct 211 He Tyr Glu Leu Gly Leu Glu Thr Gly His Wing Thr Tyr Glu Thr Ser 25 30 35 ggt ccc acg tgg gac cag ttc tc caa tct aaa atc gat gtc acc gtc 259 Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys He Met Asp Thr Val 40 45 50 atg gtg gcg gta gaa aac aac gac ccg gac ttc atc ctc gga tgg gtg 307 Met Val Wing Val Glu Asn Asn Asp Pro Asp Phe He Leu Gly Trp Val 55 60 65 cct gct cct gt cca att tea age cg cat ca g gtg gtg gag gtg gag 355 Ser Wing Ala Pro Be Ser Arg Gln Val Phe His Gly Val Val Glu 70 75 80 85 gat tcc atc tat atc cac ccc cag ggc caa ggc cga gga atc ggc ggc 403 Asp Ser He Tyr He His Pro Gln Gly Gln Gly Arg Gly He Gly Gly 90 95 100 gct ttg ctc gac gcc ctt atc acc tac tgc gaa age aac ggc atc tgg 451 Wing Leu Leu Asp Wing Leu He Thr Tyr Cys Glu Being Asn Gly He Trp 105 110 115 tcg atc cac tcc tgg atc ttc ccg gaa aac ctc ggt tct gcg aaa ctg 499 Ser He His Ser Trp He Phe Pro Glu Asn Leu Gly Ser Ala Lys Leu 120 125 130 cat gaa tcg aag ggc ttc gtg aag gtg ggc acc atg cac caa atg gca 547 His Glu Ser Lys Gly Phe Val Lys Val Gly Thr Met His Gln Met Wing 135 140 145 agg atg ccc tac ggc gag atg gaa gga caa tgg cgc gat tgt gat ctg 595 Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp Cys Asp Leu 150 155 160 165 tgg gag tgc ctc tta tcc gtt cca gag caa gct caa agt tcc 637 Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Wing Gln Ser Ser 170 175 taaagcaatt taaatctgac ttt 660 < 210 > 20 < 211 > 179 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 20 Met Val Glu Arg Asp Phe Thr He Arg Pro He Arg Glu Gly Asp Phe 1 5 10 15 Pro Gln Val Arg Asp He Tyr Glu Leu Gly Leu Glu Thr Gly His Wing 20 25 30 Thr Tyr Glu Thr Ser Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys 35 40 45 He Met Asp Thr Val Met Val Wing Val Glu Asn As-n Asp Pro Asp Phe 50 55 60 He Leu Gly Trp Val Ser Ala Ala Pro He Ser Ser Arg Gln Val Phe 65 70 75 80 His Gly Val Val Glu Asp Ser He Tyr He His Pro Gln Gly Gln Gly 85 90 95 Arg Gly He Gly Gly Ala Leu Leu Asp Ala Leu He Thr Tyr Cys Glu 100 105 110 Ser Asn Gly He Trp Ser He His Ser Trp He Phe Pro Glu Asn Leu 115 120 125 Gly Ser Ala Lys Leu His Glu Ser Lys Gly Phe Val Lys Val Gly Thr 130 135 140 Met His Gln Met Ala Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp 145 150 155 160 Arg Asp Cys Asp Leu Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Wing 165 170"175 Gln Ser Ser < 210 > 21 < 211 > 606 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 >; < 221 > CDS < 222 > (101) .. (583) < 223 > FRXA00575 < 400 > 21 gcatgtccgg ttgccgatct atttaaatac caggacaatt gcgtgcatgg ttgaaagaga 60 cttcactatc cgaccaatcc gcgagggtga tttccctcag gtg agg gac atc tac 115 Val Arg Asp He Tyr 1 5 gaa ttg ggc ctg gag acg gga cat gcg act tat gag act tct ggt ccc 163 Glu Leu Gly Leu Glu Thr Gly His Ala Thr Tyr Glu Thr Ser Gly Pro October 15 20 acg tgg gac cag ttc aka caa tct aaa atc atg gat acc gtc atg gtg 211 Thr Trp Asp Gln Phe Ser Gln Ser Lys He Met Asp Thr Val Met Val 25 30 35 gta gcg gaa aac aac gac ccg gac ttc atc ctc gga tgg gtg tct gct 259 Ala Val Glu Asn Asn Asp Pro Asp Phe He Leu Gly Trp Val Ser Ala 40 45 50 cca gct att tea age cga cag gtt ttc cat gga gtg gtg gaa gat aka 307 Ala Pro He Ser Ser Arg Gln Val Phe His Gly Val Val Glu Asp Ser 55 60 65 atc tat atc cac ccc cag GGC CAA GGC CGA GGA atc GGC GGC GCT ttg 355 I Tyr He His Pro Gln Gly Gln Gly Arg Gly I Gly Gly Ala Leu 70 75 80 85 ctc gac gcc ctt atc acc tac tgc gaa age aac ggc atc tgg tcg atc 403 Leu Asp Ala Leu He Thr Tyr Cys Glu Ser Asn Gly He Trp Ser He 90 95 100 cac aka tgg atc ttc ccg gaa aac ctc ggt tct gcg aaa ctg cat gaa 451 His Ser Trp He Phe Pro Glu Asn Leu Gly Ser Ala Lys Leu His Glu 105 110 115 tcg aag ggc ttc gtg aag gtg ggc acc atg cac caa atg gca agg atg 499 Ser Lys Gly Phe Val Lys Val Gly Thr Met His Gln Met Ala Arg Met 120 125 • 130 ccc tac ggc gag atg gaa gga caa tgg cgc gat tgt. gat ctg tgg gag 547 Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp Cys Asp Leu Trp Glu 135 140 145 tgc ctc tta aka gtt cca gag caa gct caa agt aka taaagcaatt 593 Cys Leu Leu Ser Val Pro Glu Gln Ala Gln Ser Ser 150 155 160 taaatctgac ttt 606 < 210 > 22 < 211 > 161 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 22 Val Arg Asp He Tyr Glu Leu Gly Leu Glu Thr Gly His Wing Thr Tyr 1 5 10 15 • Glu Thr Ser Gly Pro Thr Trp Asp Gln Phe Ser Gln Ser Lys He Met 20 25 30 Asp Thr Val Val Val Ala Val Glu Asn Asn Asp Pro Asp Phe He Leu 35 40 45 Gly Trp Val Ser Wing Wing Pro Be Ser Arg Gln Val Phe His Gly 50 55 60 Val Val Glu Asp Ser He Tyr He His Pro Gln Gly Gln Gly Arg Gly 10 65 70 75 80 He Gly Gly Ala Leu Leu Asp Ala Leu He Thr Tyr Cys Glu Ser Asn 85 90 95 • Gly He Trp Ser He His Ser Trp He Phe Pro Glu Asn Leu Gly Ser 100 105 110 Ala Lys Leu His Glu Ser Lys Gly Phe Val Lys Val Gly Thr Met His 115 120 125 Gln Met Ala Arg Met Pro Tyr Gly Glu Met Glu Gly Gln Trp Arg Asp 15 130 135 140 Cys Asp Leu Trp Glu Cys Leu Leu Ser Val Pro Glu Gln Ala Gln Ser 145 150 155. 160 Ser < 210 > 23 < 211 > 1575 < 212 > DNA 20 < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1552) < 223 > RXN01345 < 400 > 23 cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60 ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115 Met Arg Phe Gly Leu 25 1 5 gac ttg gga act acc cgc here atc gcg gcc gcc gtg gac cgc gga aac 163 Asp Leu Gly Thr Thr Arg Thr He Wing Wing Wing Val Asp Arg Gly Asn 10 15 20 tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211 Tyr Pro He Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe 25 30 35 att cca tct gtg gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259 He Pro Ser Val Val Ala Leu Lys Wing Asp Arg He Val Wing Gly Trp 40 45 50 gat gct att gag gtt ggg cag gac cac ect tcc ttc gta cgt tct ttc 307 Asp Ala He Glu Val Gly Gln Asp His Pro Ser ..Phe Val Arg Ser Phe 55 60 ''65 aaa cgc cta ctc tct gaa ccc aat gtc acg gaa' gcc acc ccg gtc tac 355 Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Wing Thr Pro Val Tyr 70 75 80 85 ttg ggc gat cat gta cac ect ttg ggc gcc gtc ctg gag gct ttt gcg 403 Leu Gly Asp His Val His Pro Leu Gly Ala Val Leu Glu Ala Phe Wing 90 95 100 gaa aac gtg gtc act gcg ctg cgt gca ttt cag a.cg caa ttg gga gat 451 Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp 105 110 115 accc ccg atc gaa gta gtc att ggt gtg ccc gcc aac ccc cc age 499 Thr Ser Pro He Glu Val Val He Gly Val Pro Wing Asn Ser His Ser 120 125 130 gcc cag cga ctc ctc acc atg tcc gcc ttc age gcc gc gc atc acc 547 Wing Gln Arg Leu Leu Thr Met Be Wing Phe Be Wing Thr Gly He Thr 135 140 145 gtt gtc gtc gtc gtc aat gag ccc age gcc gc gct ttc gag tac acc 595 Val Val Gly Leu Val Asn Glu Pro Be Ala Wing Wing Phe Glu Tyr Thr 150 155 160 '' 165 cac cgc cac gcc cgc acc tta aac tcc aag cgc caa * gcc atc gtg gtt 643 His Arg His Wing Arg Thr Leu Asn Ser Lys Arg Gl? Ala He Val Val 170 175, 180 tat gat ttg gga ggc gga here ttc gac tcc tcg ctc atc cgc atc gac 691 Tyr Asp Leu Gly Gly Gly Thr Phe Asp Being Ser Leu He Arg He Asp 185 190 195 ggc acc cac gac gtt gtg tcc tcc att ggc att cgc ctt ggt 739 Gly Thr His His Glu Val Val Ser Ser He Gly He Ser Arg Leu Gly 200 205 210 ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787 Gly Asp Asp Phe Asp Glu He Leu Leu Gln Cys Ala Leu Lys Ala Ala 215 220 225 ggc aga cag cac gat gcg ttt ggc aag cgt gct a-aa aac acg ctt ctc 835 Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu 230 235 240 245 gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883 Asp Glu Be Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg 250 255 260 ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931 Leu Val Leu Glu He Gly Asp Asp Asp He Thr Val Pro Val Asn Lys 265 270 275 ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979 Phe Tyr Glu Ala Wing Thr Pro Leu Val Glu Lys Ser Leu Ser He Met 280 285. 290 gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca ggc 1027 Glu Pro Leu He Gly Val Asp Asp Leu Lys Asp Ser Asp He Wing Gly 295 300 305 ate tac ctt gtt ggt gga gga tcc tcg ctc cca qtc gtt tcc agg ttg 1075 He Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu 310 315 320 -. 325 ctc cgc gag cgt tgc cgc cgc gtc ccc cac ccc ccc tcc ccc tea 1123 Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser 330 335 gt 340 tcc act gcg gtg gtg gtc ctg gcc gcg gcg cctc ect tcc tct ggt 1171 Gly Ser Thr Wing Val Gly Leu Wing Wing Wing Asp Pro Ser Ser Gly 345 350 355 ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219 Phe His Leu Arg Asp Arg Val Wing Arg Gly He Gly Val Phe Arg Glu 360 365 370 cac gat tct ggt cgt gcc gtg age ttt gac ccg ctg ctg atc gcc ccg gac 1267 His Asp Ser Gly Arg Wing Val Ser Phe Asp Pro Leu He Wing Pro Asp 375 380 385 acc gat tct gcg acc gtg gcg aaa cga tgc tac aag gcg gtg cac aac 1315 Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr Lys Ala Val His Asn 390 395 400 * 405 att ggt tgg ttc agg ttc gtg gaa tac tcc acc gtg tcc gag gat ggc 1363 He Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr Val Ser Glu Asp Gly 410 415"." 420 age ccc gga gat att tcc ctg ctc agt gaa atc aag att ect ttt gat 1411 Ser Pro Gly Asp Be Ser Leu Leu Ser Glu He Lys He Pro Phe Asp 425 430% 435 age tcc atc acc gat gtg gat gct acc gag att tea cgt ttc gat ggc 1459 Ser Ser He Thr Asp Val Asp Ala Thr Glu He Ser Arg Phe Asp Gly 440 445 450 cca gaa gta gaa gaa acc atc here gtc aat gac aac ggc gtg gct tcc 1507 Pro Glu Val Glu Glu Thr He Thr Val Asn Asp Asn Gly Val Ala Ser 455 460 465 att tcc atc aag ata ctc ggc ggc gtt acc gtc gag cac here att 1552 He Be He He Lys He Leu Gly Gly Val Thr Val Glu His Thr He 470 475 480 'tagttaccat tttggtgctg gtg * 1575 < 210 > 24 < 211 > 484 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 24 Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr He Wing Wing 1 5 10 15 Val Asp Arg Gly Asn Tyr Pro He Val Thr Val Glu Asp Ser Leu Gly 20 25 30 Asp Thr His Asp Phe He Pro Ser Val Val Wing Leu Lys Wing Asp Arg 35 40 45 He Val Wing Gly Trp Asp Wing He Glu Val Gly Gln Asp His Pro Ser 50 55 60 Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu 65 70 75 80 Wing Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val 85 90 95 Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln 100 105 110 Thr Gln Leu Gly Asp Thr Ser Pro He Glu Val Val He Gly Val Pro 115 120 125 Wing Asn Ser His Wing Gln Arg Leu Leu Thr Met Ser Wing Phe Ser 130 135 140 Wing Thr Gly He Thr Val Val Gly Leu Val Asn Glu Pro Wing Wing 45 150 155 160 Wing Phe Glu Tyr Thr His Arg His Wing Arg Thr Leu Asn Ser Lys Arg 165 170 175 Gln Ala He Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser 180 185 • 190 Leu He Arg He Asp Gly Thr His His Glu Val Val Ser Ser He Gly 195 200 205 H e Ser Arg Leu Gly Gly Asp Asp Phe Asp Glu He Leu Leu Gln Cys 210 215 220 Wing Leu Lys Wing Wing Gly Arg Gln His Asp Wing Phe Gly Lys Arg Wing 225 230 235 240 Lys Asn Thr Leu Leu Asp Glu Be Arg Asn Ala Lys Glu Ala Leu Val 245 250 255 Pro Gln Ser Arg Arg Leu Val Leu Glu He Gly Asp Asp Asp He Thr 260 265 270 Val Pro Val Asn Lys Phe Tyr Glu Ala Wing Thr Pro Leu Val Glu Lys 275 280 285 Ser Leu Ser He Met Glu Pro Leu He Gly Val Asp Asp Leu Lys Asp 290 295 300 Ser Asp He Wing Gly He Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro 305 310 315 • 320 Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg 325 330 '335 Ser Pro Phe Pro Ser Gly Ser Thr Wing Val Gly Leu Wing He Wing Wing 340 345 - 350 Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Wing Arg Gly He 355 360 365 Gly Val Phe Arg Glu His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro 370 375 380 Leu He Wing Pro Asp Thr Asp Ser Wing Thr Val Wing Lys Arg Cys Tyr 385 390 395 400 Lys Ala Val His Asn He Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr 405 410 415 Val Ser Glu Asp Gly Ser Pro Gly Asp Be Ser Leu Leu Ser Glu He 420 425 430 Lys He Pro Phe Asp Ser Ser He Thr Asp Val Asp Ala Thr Glu He 435 440 445 Ser Arg Phe Asp Gly Pro Glu Val Glu Glu Thr He Thr Val Asn Asp 450 455 4.60 Asn Gly Val Ala Ser He Be He Lys He Leu Gly Val Thr Val 465 470 475 480 Glu His Thr He < 210 > 25 < 211 > 1267 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101). . (1267) < 223 > FRXA01345 < 400 > 25 cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60 ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115 Met Arg Phe Gly Leu 1 5 gac ttg gga act acc cgc here atc gcg gcc gcc gtg gac cgc gga aac 163 Asp Leu Gly Thr Thr Arg Thr He Ala Ala Ala Val Asp Arg Gly Asn 10 15. 20 tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211 Tyr Pro He Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe 25 30 35 att cca tct gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259 He Pro Ser Val Val Ala Leu Lys Wing Asp Arg He Val Wing Gly Trp 40 45 50 gat gct att gag gtt ggg cag gac cac ect tcc tcc gta cct tct ct 307 Asp Ala He Glu Val Gly Gln Asp His Pro Ser Phe Val Arg Ser Phe 55 60 65 aaa cgc cta ctc tct gaa ccc aat gtc acg gaa gcc acc ccg gtc tac 355 Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Wing Thr Pro Val Tyr 70 75 80 85 ttg ggc gat cat gta cac ect ttg ggc gcc gtc ctg gag gct ttt gcg 403 Leu Gly Asp His Val His Pro Leu Gly Wing Val Leu Glu Wing Phe Wing 90 95 100 gaa aac gtg gtc act gcg ctg cgt gca ttt cag acg caa ttg gga gat 451 Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp 105 110 115 acc tcc ccg atc gaa gta gtc att ggt gtg ccc gcc aac tcc cac age 499 Thr Ser Pro He Glu Val Val He Gly Val Pro Ala Asn Ser His Ser 120 125 • 130 gcc cag cga ctg ctc acc atg tcc gcc ttc age gcc here ggc atc acc 547 Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser Ala Thr Gly He Thr 135 140 145 gtt gtc ggt ttg gtc aat gag ccc age gcc gc gct tcc gag tac acc 595 Val Val Gly Leu Val Asn Glu Pro Be Ala Wing Wing Phe Glu Tyr Thr 150 155 160 165 cac cgc cac gcc cgc acc tta aac tcc aag cgc caa gcc atc gtg gtt 643 His Arg His Wing Arg Thr Leu Asn Ser Lys Arg Gln Wing He Val Val 170 175 180 tat gat ttg gga ggc gga here ttc gac tcc tcg ctc atc cgc atc gac 691 Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser Leu He Arg He Asp 185 190 195 ggc acc cac gac gtt gtg tcc tcc att ggc att tea cgc ctt ggt 739 Gly Thr His His Glu Val Val Ser Ser He Gly He Ser Arg Leu Gly 200 205 _ 210 ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787 Gly Asp Asp Phe Asp Glu He Leu Leu Gln Cys Ala Leu Lys Ala Ala 215 220 225 ggc aga cag gat gcg ttt ggc aag cgt gct aaa aac acg ctt ctc 835 Gly Arg Gln His Asp TO the Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu 230 235 240 245 gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883 Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg 250 255 260 ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931 Leu Val Leu Glu He Gly Asp Asp Asp He Thr Val Pro Val Asn Lys 265 270 • 275 ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979 Phe Tyr Glu Wing Wing Thr Pro Leu Val Glu Lys Ser Leu Ser Met 280 280 285 290 gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca gcc 1027 Glu Pro Leu He Gly Val Asp Asp Leu Lys Asp Ser Asp He Wing Gly 295 300 305 atc tac ctt gtt ggt gga gga tcc tcg ctc cca ccc gt tcc agg ttg 1075 He Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu 310 315 320 325 ctc cgc gag cgt ttc ggc cgc cgt gtc cac cgc tcc cca ttc ccc tea 1123 Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser 330 335 340 ggt tcc act gcg gtg ggt ctg gcc atc gcg gct gac ect tcc tct ggt 1171 Gly Ser Thr Wing Val Gly Leu Wing Wing Wing Asp Pro Ser Ser Gly 345 350 355 ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219 Phe His Leu Arg Asp Arg Val Wing Arg Gly He Gly Val Phe Arg Glu 360 365 .370 cac gat tct ggt cgt gcc gtg age ttt gac ccg ctg atc gcc ccg gac 1267 His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro Leu He Ala Pro Asp 375 380 385 < 210 > 26 < 211 > 389 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 26 Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr He Ala Wing Wing 1 5 10 15 Val Asp Arg Gly Asn Tyr Pro He Val Thr Val Glu Asp Ser Leu Gly 20 25 30 Asp Thr His Asp Phe He Pro Ser Val Val Ala Leu Lys Wing Asp Arg 35 40 45 He Val Wing Gly Trp Asp Wing He Glu Val Gly Gln Asp His Pro Ser 50 55 .60 Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu 65 70 75 80 Ala Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val 85 90 95 Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln 100 105 110 Thr Gln Leu Gly Asp Thr Ser Pro He Glu Val Val He Gly Val Pro 115 120 125 Ala Asn Ser His Ser Wing Gln Arg Leu Leu Thr Met Ser Wing Phe Ser 130 135 140 Wing Thr Gly He Thr Val Val Gly Leu Val Asn Glu Pro Wing Ala 145 150 155 • 160 Wing Phe Glu Tyr Thr His Arg His Wing Arg Thr Leu Asn Ser Lys Arg 165 170. 175 Gln Ala He Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Being Ser 180 185 190 Leu He Arg He Asp Gly Thr His His Glu Val Val Be Ser He Gly 195 200 205 He Be Arg Leu Gly Gly Asp Asp Phe Asp Glu He Leu Leu Gln Cys 210 215 220 Wing Leu Lys Wing Wing Gly Arg Gln His Asp Wing Phe Gly Lys Arg Wing 225 230 235 240 Lys Asn Thr Leu Leu Asp Glu Be Arg Asn Ala Lys Glu Ala Leu Val 245 250 255 Pro Gln Ser Arg Arg Leu Val Leu Glu He Gly Asp Asp Asp He Thr 260 265 270 Val Pro Val Asn Lys Phe Tyr Glu Ala Wing Thr Pro Leu Val Glu Lys 275 280 285 Ser Leu Ser He Met Glu Pro Leu He Gly Val Asp Asp Leu Lys Asp 290 295 300 Ser Asp He Wing Gly He Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro 305 310 315 320 Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg 325 330 335 Ser Pro Phe Pro Ser Gly Ser Thr Wing Val Gly Leu Wing Wing Wing 340 345 350 Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Wing Arg Gly He 355 360 365 Gly Val Phe Arg Glu His Asp Ser Gly Arg Wing Val Ser Phe Asp Pro 370 375 380 Leu He Wing Pro Asp 385 < 210 > 27 < 211 > 1308 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1285) < 223 > RXA02541 < 400 > 27 atccgccggt gtccggacaa caaaacttgc aacacaagat aacttaagaa attgcataca 60 attcaccgca tataagactc atggaaggag gggatgccca gtg aac aac age gaa 115 Val Asn Asn Ser Glu 1 5 tgg gca aat aag aac tat tac gca gac ctg ggg gtc tcc tcg tcc gct 163 Trp Wing Asn Lys Asn Tyr Tyr Wing Asp Leu Gly Val Being Ser Wing 10 15/20 tea gaa gat gag atc aaa aag gct tac cgc aag ctc gcc agg gaa aat 211 Ser Glu Asp Glu He Lys Lys Wing Tyr Arg Lys Leu Wing Arg Glu Asn 25 30 35 cac cca gat aaa aat cca ggt gac aag gcc gact gat cga ttc aaa 259 His Pro Asp Lys Asn Pro Gly Asp Lys Ala Wing Glu Asp Arg Phe Lys 40 45 50 aaa gcg gcc gag gca tat gac gta ctt ggt gat gac aag aaa cga aaa 307 Lys Ala Ala Glu Ala Tyr Asp Val Leu Gly Asp Asp Lys Lys Arg Lys 55 60 65 gaa tac gac gac ctc aaa gca ctt cta gct tct ggt gga atc cgc gga 355 Glu Tyr Asp Glu Leu Lys Ala Leu Leu Ala Ser Gly Gly He Arg Gly 70 75 80 85 gga ttc gga age gga ggt gcg gga tcc ccc ggc ggg ttt cgc acg tcg 403 Gly Phe Gly Ser Gly Gly Wing Gly Phe Pro Gly Gly Phe Arg Thr Ser 90 95 100 acg gga gga ttc gac acc tea gac ctc ttc gga gga gga gga caga ggt gga 451 Thr Gly Gly Phe Asp Thr As Asp Leu Phe Gly Gly Gly Gly Gly Gly 105 110 115 ggg ttt tcg acg gac ggc ggc tgc gtc gat atc tgc ggt gtc ctt ttc 499 Gly Phe Ser Thr Asp Gly Gly Leu Gly Asp He Phe Gly Gly Leu Phe 120 125 130 aac cgc ggc gct gct tct cac cag tea gct agg ccg acg cgg ggg gcg 547 Asn Arg Gly Wing Gly Ser His Gln Ser Wing Arg Pro Thr Arg Gly Wing 135 140 14'5 gat gta caa acc gaa ata act ctc tcg ttt gtt gag gca gcg aaa ggc 595 Asp Val Gln Thr Glu He Thr Leu Ser Phe Val Glu Ala Ala Lys Gly 150 155 160 165 acg acc atc cca gtg gaa ctc acc ggc gat gcg ccc tgc aac acc tgc 643 Thr Thr He Pro Val Glu Leu Thr Gly Asp Wing Pro Cys Asn Thr Cys 170 175 180 cac gga tcg ggc tcc aaa tea ggc cac ccc gca aaa tgt gga acc tgt 691 His Gly Ser Gly Ser Lys Ser Gly His Pro Wing Lys Cys Gly Thr Cys 185 190 195 gat gga acc gga ttc acc tct gag aac aag ggt gct ttc gga ttc tcc 739 Asp Gly Thr Gly Phe Thr Ser Glu Asn Lys Gly Wing Phe Gly Phe Ser 200 205 210 gct cca tgt gca acc tgt ggt ggt act ggt gaa ata atc act gat ect 787 Wing Pro Cys Wing Thr Cys Gly Gly Thr Gly Glu He He Thr Asp Pro 215 220 225 tgc gat aac tgc cac ggc cga ggc acc gtc cgg aag tct cgt tcc atc 835 Cys Asp Asn Cys His Gly Arg Gly Thr Val Arg Lys Ser Arg Ser He 230 235 240 245 acc gtg cgt atc cca act ggt gtg gaa gat gga cag aaa gtt cgt ctt 883 Thr Val Arg He Pro Thr Gly Val Glu Asp Gly Gln Lys Val Arg Leu 250 255 260 gca ggc caa ggc gaa gca gca cca aat ggc aaa cca gcg ggc gat ctc 931 Wing Gly Gln Gly Glu Wing Gly Pro Asn Gly Lys Pro Wing Gly Asp Leu 265 270 275 ttt gtg aaa gtc cac gtg aaa aag gac gat gtg ttc here cgc gac ggc 979 Phe Val Lys Val His Val Lys Lys Asp Asp Val Phe Thr Arg Asp Gly 280 285 290 age aac att ttg atc acc att ccc gtg age ttc age gag ctg gct ttg 1027 Ser Asn He Leu He Thr He Pro Val Ser Phe Ser Glu Leu Ala Leu 295 300 305 ggt ggc gct att tct gtg cca acg ctc aac aag ect gta aaa ctc aag 1075 Gly Gly Ala He Ser Val Pro Thr Leu Asn Lys Pro Val Lys Leu Lys 310 315 320 325 cta ect gcg gga acg cca gat ggt cgt act ttg cgt gta cgc ggt cgc 1123 Leu Pro Wing Gly Thr Pro Asp Gly Arg Thr Leu Arg Val Arg Gly Arg 330 335 340 ggt atc gaa gca cgt gat tcc act ggt gat ctg ctg gtt here gtc cag 1171 Gly He Glu Wing Arg Asp Ser Thr Gly Asp Leu Leu Val Thr Val Gln 345 350 355 gtt tct gtc ccg aag aat ctg gat gac aac gct gcg gaa gct ctc cgc 1219 Val Ser Val Pro Lys Asn Leu Asp Asp Asn Ala Ala Glu Ala Leu Arg 360 365 370 gca tat gct gaa gca gaa act aat tea ggt ttt gat ccc cgc gct aac 1267 Ala Tyr Ala Glu Ala Glu Thr Asn Ser Gly Phe? Sp Pro Arg Ala Asn 375 380 385 tgg gcg ggc cag aac cgc tagacgttct ctttgagaaa gga 1308 Trp Ala Gly Gln Asn Arg 390 395 < 210 > 28 < 211 > 395 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 28 Val Asn Asn Ser Glu Trp Wing Asn Lys Asn Tyr Tyr Wing Asp Leu Gly 1 5 10 15 Val Ser Ser Wing Ser Glu Asp Glu He Lys Lys Wing Tyr Arg Lys 20 25 30 Leu Wing Arg Glu Asn His Pro Asp Lys Asn Pro Gly Asp Lys Ala Ala 35 40 45 Glu Asp Arg Phe Lys Lys Ala Ala Glu Ala Tyr Asp Val Leu Gly Asp 50 55 60 Asp Lys Lys Arg Lys Glu Tyr Asp Glu Leu Lys Ala Leu Leu Ala Ser 65 70 75 80 Gly Gly He Arg Gly Gly Ghe Phe Gly Be Gly Gly Wing Gly Phe Pro Gly 85 90 95 Gly Phe Arg Thr Ser Thr Gly Gly Phe Asp Thr Ser Asp Leu Phe Gly 100 105 _ 110 Gly Gly Gln Gly Gly Ghe Phe Ser Thr Asp Gly Gly Leu Gly Asp He 115 120 125 Phe Gly Gly Leu Phe Asn Arg Gly Wing Gly Ser His Gln Ser Wing Arg 130 135 140 Pro Thr Arg Gly Wing Asp Val Gln Thr Glu He Thr Leu Ser Phe Val 145 150 155 160 Glu Ala Ala Lys Gly Thr Thr He Pro Val Glu Leu Thr Gly Asp Ala 165 170 175 Pro Cys Asn Thr Cys His Gly Ser Gly Ser Lys Ser Gly His Pro Wing 180 185 190 Lys Cys Gly Thr Cys Asp Gly Thr Gly Phe Thr Ser Glu Asn Lys Gly 195 200 205 Wing Phe Gly Phe Ser Wing Pro Cys Wing Thr Cys Gly Gly Thr Gly Glu 210 215 220- He He Thr Asp Pro Cys Asp Asn Cys His Gly Arg Gly Thr Val Arg 225 230 235 240 Lys Ser Arg Be He Thr Val Arg He Pro Thr Gly Val Glu Asp Gly 245 250 255 Gln Lys Val Arg Leu Wing Gly Gln Gly Glu Wing Gly Pro Asn Gly Lys 260 265 270 Pro Wing Gly Asp Leu Phe Val Lys Val His Val Lys Lys Asp Asp Val 275 280 285 Phe Thr Arg Asp Gly Ser Asn He Leu He Thr He Pro Val Ser Phe 290 295 300 Ser Glu Leu Ala Leu Gly Gly Ala He Ser Val Pro Thr Leu Asn Lys 305 310 315 320 Pro Val Lys Leu Lys Leu Pro Wing Gly Thr Pro Asp Gly Arg Thr Leu 325 330 335 Arg Val Arg Gly Arg Gly He Glu Wing Arg Asp Ser Thr Gly Asp Leu 340 345 '350 Leu Val Thr Val Gln Val Ser Val Pro Lys Asn Leu Asp Asp Asn Wing 355 360 365 Wing Glu Wing Leu Arg Wing Tyr Wing Glu Wing Glu Thr Asn Ser Gly Phe 370 375 380 Asp Pro Arg Wing Asn Trp Wing Gly Gln Asn Arg 385 390 395 < 210 > 29 < 211 > 777 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (754) < 223 > RXA02542 < 400 > 29 ccaggctgac gcaggtgcag aaggcgctgc agatgacaat gttgttgacg ctgaagttgt 60 cgaagacgac gcagctgaca atggtgagga caagaagtaa atg act acc ect aac 115 Met Thr Thr Pro Asn 1 5 gga atg ccc gac aat ect ggg gat ect gaa aat acc gat gat cca gag gca 163 Gly Met Pro Asp Asn Pro Gly Asp Pro Glu Asn Thr Asp Pro Glu Wing 10 15 20 ac tct gct gat cgt gct gag cag gca gct gaa gaa gca gct gcc cgc 211 Thr Ser Wing Asp Arg Wing Glu Gln Wing Wing Glu Glu Wing Wing Wing Arg 25 30 35 cag gcg gag gaa tct cca ttt gga cag gcc tea gag gaa gaa att tct 259 Gln Ala Glu Glu Ser Pro Phe Gly Gln Ala Ser Glu Glu Glu He Ser 40 45 50 cca gag ctc gaa gca gag atc aat gat ctt cta tea gat gtt gat cca 307 Pro Glu Leu Glu Wing Glu He Asn Asp Leu Leu Ser Asp Val Asp Pro 55 60 65 gat ttg gat ggc gat ggt gaa gtg tcc gct gta gaa here cag ctt gcc 355 Asp Leu Asp Gly Asp Gly Glu Val Ser Wing Val Glu Thr Gln Leu Ala 70 75 80 85 gaa cgc act gag gat ctg cag cga gtc acc gct gag tac gcc aac tac 403 Glu Arg Thr Glu Asp Leu Gln Arg Val Thr Ala Siu Tyr Ala Asn Tyr 90 95 100 cgt cga cgt acc gag cgt gaa cgc cag ggc atc atc gac acc gca cgc 451 Arg Arg Arg Thr Glu Arg Glu Arg Gln Gly He He Asp Thr Ala Arg 105 110 115 gca ggt gtt gtt gt ctg ctg ctg ctg ctc gac gat ctt gac ctg 499 Gly val Val Thr Gln Leu Leu Pro Leu Leu Asp Asp Leu Asp Leu 120 125 130 gct gag cag cag ggt gac ctt aac gaa ggt ccg ctg aag tea ctg tct 547 Wing Glu Gln His Gly Asp Leu Asn Glu Gly Pro Leu Lys Ser Leu Ser 135 140 145 gac aag ctg atc aac atc ctg ggt gga ttg aag gtg gaa tcc tcc ggc 595 Asp Lys Leu He Asn He Leu Gly Gly Leu Lys Val Glu Ser Phe Gly 150 155 160 165 gag atc ggc gaa gca ttc gat cca gag atc cac gaa gca gta cag gat 643 Glu He Gly Glu Wing Phe Asp Pro Glu He His Glu Wing Val Gln Asp 170 175 '' 180 ctc tea cag ggt gat gtc aag gtt ctg gga acg gta ctc cgc aag gga 691 Leu Ser Gln Gly Asp Val Lys Val Leu Gly Thr Val Leu Arg Lys Gly 185 190 195 tac cgc ctc ggc gac cgc gtc atc cgc acc gca atg gtc ctc att ggg 739 Tyr Arg Leu Gly Asp Arg Val He Arg Thr Ala Met Val Leu He Gly 200 205 210 gat cca gag gag age tagagagact aagtctctta gtg 777 Asp Pro Glu Glu Ser 215 < 210 > 30 < 211 > 218 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 30 Met Thr Thr Pro Asn Gly Met Pro Asp Asn Pro Gly Asp Pro Glu Asn 1 5 10 15 Thr Asp Pro Glu Wing Thr Ser Wing Asp Arg Wing Glu Gln Wing Wing Glu 20 25 30 Glu Wing Wing Wing Arg Gln Wing Glu Glu Wing Pro Phe Gly Gln Wing Ser 35 40 45 Glu Glu Glu He Ser Pro Glu Leu Glu Wing Glu He Asn Asp Leu Leu 50 55 60 Be Asp Val Asp Pro Asp Leu Asp Gly Asp Gly Val Val Ser Wing Val 65 70 75 80 Glu Thr Gln Leu Wing Glu Arg Thr Glu Asp Leu Gln Arg Val Thr Wing 85 90 95 Glu Tyr Wing Asn Tyr Arg Arg Arg Thr Glu Arg Glu Arg Gln Gly He 100 105 110 He Asp Thr Wing Arg Wing Gly Val Val Thr Gln Leu Leu Pro Leu Leu 115 120? 125 Asp Asp Leu Asp Leu Wing Glu Gln His Gly Asp Leu Asn Glu Gly Pro 130 135 140 Leu Lys Ser Leu Ser Asp Lys Leu He Asn He Leu Gly Gly Leu Lys 145 150 155 160 Val Glu Ser Phe Gly Glu He Gly Glu Wing Phe Asp Pro Glu He His 165 170 175 Glu Wing Val Gln Asp Leu Ser Gln Gly Asp Val Lys Val Leu Gly Thr 180 185. 190 Val Leu Arg Lys Gly Tyr Arg Leu Gly Asp Arg Val He Arg Thr Wing 195 200 205 Met Val Leu He Gly Asp Pro Glu Glu Ser 210 215 < 210 > 31 < 211 > 1977 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1954) < 223 > RXN02543 < 400 > 31 ctcaatgagg agtttttctt accggcgaaa gtcggtggga agcaagtcaa agctcaagcc 60 gtggacagta ctaaaatcac ctaaaacagg aggcaccatt atg gga cgt gca gta 115 Met Gly Arg Ala Val 1 5 gga att gac ctt gga acc accc aac tct gtg gtt tc gta ctt gaa ggc 163 Gly He Asp Leu Gly Thr Thr Asn Val Val Ser Val Leu Glu Gly 10 15 20 ggc gag cca gta gt atc gca aac gca gaa ggc tea cgc acc action 211 Gly Glu Pro Val Val He Wing Asn Wing Glu Gly Ser Arg Thr Thr Pro 25 30 35 tcc gtc gtt gca ttc gca aag aac ggt gaa gtt cta gtc ggc cag tcc 259 Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val Leu Val Gly Gln Ser 40 45 50 gct aag aac cag gcg gtc acc aac gtt gac cgc acc att cgc tcc gtc 307 Wing Lys Asn Gln Wing Val Thr Asn Val Asp Arg Thr He Arg Ser Val 55 60 65 aag cgc cac atc ggc acc gac tgg tcc gtt gct atc gat gac aag aac 355 Lys Arg His He Gly Thr Asp Trp Ser Val Wing He Asp Asp Lys Asn 70 75 80 85 tac acc cag gaa atc tcg gct cgt acc ctg atg aag ctg aag cgc 403 Tyr Thr Ser Gln Glu Be Ser Ala Arg Thr Leu Met Lys Leu Lys Arg 90 95 100 gac gct gaa gca tac ggc gag gac gtc act gat gct gtt att acc 451 Asp Wing Glu Wing Tyr Leu Gly Glu Asp Val Thr Asp Wing Val He Thr 105 110 115 gtt ect gca tac tcc gag gac tea cag cgc cag gca acc aag gaa gct 499 Val Pro Wing Tyr Phe Glu Asp Ser Gln Arg Gln Wing Thr Lys Glu Wing 120 125 130 ggt cag atc gca ggc ctt aac gtt ctg cgt att gtt aac gag cca acc 547 Gly Gln He Wing Gly Leu Asn Val Leu Arg He Val Asn Glu Pro Thr 135 140 145 gcg gct gca ctt gca tac ggc ctt gag aag ggc gag cag gag cag acc 595 Wing Wing Wing Leu Wing Tyr Gly Leu Glu Lys Gly Gluc Gln Glu Gln Thr 150 155 160 165 att ctg gta ttc gac ctc ggt ggc ggc acc ttc gac gtc tcc ctc cta 643 He Leu Val Phe Asp Leu Gly Gly Thr Phe Asp Val Ser Leu Leu 170 175 180 gag atc ggc gac ggt gtt gtt gag gtt cgc gca acc tcc ggc gat aac 691 Glu He Gly Asp Gly Val Val Glu Val Arg Ala Thr Ser Gly Asp Asn 185 190 195 gag ctc ggt ggc gac gac tgg gat cag cgt atc gtt gac tgg ctg gta 739 Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg He Val Asp Trp Leu Val 200 205 210 gag aag ttc cag tcc tcc aac ggc att gac ctg acc aag gac aag at 787 Glu Lys Phe Gln Ser Ser Asn Gly He Asp Leu Thr Lys Asp Lys Met 215 220 225 gcc ctg cag cgt ctg cgt gag gca gct gag aag gca aag atc gag ctg 835 Wing Leu Gln Arg Leu Arg Glu Wing Wing Glu Lys Wing Lys He Glu Leu 230 235 240 245 tcc tct tcc cag agt gca aac atc aac ctt ect tac atc acc gtt gat 883 Being Ser Gln Ser Wing Asn He Asn Leu Pro Tyr He Thr Val Asp 250 255 260 gca gac aag aac cca ctg ttc ttg gat gag acc ctt tcc cgt gcc gag 931 Wing Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr Leu Ser Arg Ala Glu 265 270 275 ttc cag cgc atc acc cag gac ctc ctg gcc cgc acc aag act ect ttc 979 Phe Gln Arg He Thr Gln Asp Leu Leu Wing Arg Thr Lys Thr Pro Phe 280 285 290 aac cag gtt gtt aag gac gct ggc gtg tcc gtc tcg gag atc gac cac 1027 Asn Gln Val Val Lys Asp Wing Gly Val Ser Val Ser Glu He Asp His 295 300 305 gtt gtt ctc gtc ggt ggt tcc acc cgt atg ect gct gtt acc gaa ctg 1075 Val Val Leu Val Gly Gly Ser Thr Arg Met Pro Wing Val Thr Glu Leu 310 315 320 325 gtc aag gaa ctg acc ggt gga cgt gag cca aac aag ggt gtt aac cca 1123 Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn Lys Gly Val Asn Pro 330 335 340 gat gag gtt gtt gca gtt ggt gca gca ctt cag gcc ggt gtt ctc cgc 1171 Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln Ala Gly Val Leu Arg 345 350 • 355 ggc gag gtc aag gat gtt ctt ctt gac gtc acc cca ctg tcc ctc 1219 Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu 360 365 370 ggc att gag acc aag ggt ggc gtg atg acc aag ctc atc gag cgc aac 1267 Gly He Glu Thr Lys Gly Gly Val Met Thr Lys Leu He Glu Arg Asn 375 380 385 j ^ MjÜÉ acc acc atc ect acc aag cgt tcc gag acc ttc ac acc gca gag gac 1315 Thr Thr He Pro Thr Lys Arg Ser Glu Thr Phe Thr Thr Wing Glu Asp 390 395 400 405 aac cag ect tct gtt cag atc cag gtc ttc cag ggc gag cgt gaa atc 1363 Asn Gln Pro Ser Val Gln He Gln Val Phe Gln Gly Glu Arg Glu He 410 415 420 gca acc gcc aac aag ctg ctc gga tcc tcc gag ctc ggc ggc atc gca 1411 Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu Leu Gly Gly He Wing 425 430 435 ect gca cca ggc ggc gtc cca cag atc gac gtc act ttc gac atc gac 1459 Pro Wing Pro Arg Gly Val Pro Gln He Glu Val Thr Phe Asp He Asp 440 445 450 gcc aac ggc atc gtc cac gtc acc gca aag gac aag ggt act ggc aag 1507 Wing Asn Gly He Val His Val Thr Wing Lys Asp Lys Gly Thr Gly Lys 455 460 465 gaa aac acc atc acc att cag gac ggc tcc ggt ctc tcc cag gat gaa 1555 Glu Asn Thr He Thr He Gln Asp Gly Ser Gly Leu Ser Gln Asp Glu 470 475 480 485 att gat cgc atg atc aag gat gct gaa gct cac gct gat gag gac aag 1603 He Asp Arg Met He Lys Asp Wing Glu Wing His Wing TO sp Glu Asp Lys 490 495 500 aag cgc cgc gag gag cag gaa gtc cgc aac aac gct gag tcc ctg gtt 1651 Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn Ala Glu Ser Leu Val 505 510 515 tac cag acc cgc aag ttc gtt gaa gag aac tcc gag aag gtc tcc gaa 1699 Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser Glu Lys Val Ser Glu 520 525. 530 gac ctc aag gca aag gtc gaa gac gca gcc aag ggc gtt gaa gaa gca 1747 Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys Gly Val Glu Glu Ala 535 540 545 ctc aag ggc gag gac ctc gag gca atc aag gct gca gtt gag aag ctg 1795 Leu Lys Gly Glu Asp Leu Glu Ala He Lys Wing Wing Val Glu Lys Leu 550 555 560 * 565 aac acc gag tcc cag gaa atg ggt aag gct atc tac gac gct gac gct 1843 Asn Thr Glu Ser Gln Glu Met Gly Lys Wing He Tyr Glu Wing Ala Asp Wing 570 575 580 gct gct ggt gca ggt acc ggt cag gc ggt gca ggt gag ggc gct gca gat 1891 Wing Wing Gly Wing Thr Gln Wing Asp Wing Gly Wing Glu Gly Wing Wing Asp 585 590 595 gac aat gtt gtt gac gct gaa gtt gtc gaa gac gac gca gct gac aat 1939 Asp Asn Val Val Asp Ala Glu Val Val Glu Asp Asp Ala Wing Asp Asn 600 605 610 ggt gag gac aag aag taaatgacta cccctaacgg aat 197" Gly Glu Asp Lys Lys 615 < 210 > 32 < 211 > 618 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 32 Met Gly Arg Wing Val Gly He Asp Leu Gly Thr Thr Asn Ser Val Val 1 5 10 15 Ser Val Leu Glu Gly Gly Glu Pro Val Val He Wing Asn Wing Glu Gly 20 25 30 Ser Arg Thr Thr Pro Ser Val Val Wing Phe Wing Lys Asn Gly Glu Val 35 40 45 Leu Val Gly Gln Ser Wing Lys Asn Gln Wing Val Thr Asn Val Asp Arg 50 55 60 Thr He Arg Ser Val Lys Arg His He Gly Thr Asp Trp Ser Val Wing 65 70 75 80 He Asp Asp Lys Asn Tyr Thr Ser Gln Glu He Ser Wing Arg Thr Leu 85 90 '95 Met Lys Leu Lys Arg Asp Wing Glu Wing Tyr Leu Gly "Glu Asp Val Thr 100 105 110 Asp Wing Val He Thr Val Pro Wing Tyr Phe Glu Asp Ser Gln Arg Gln 115 120 125 Wing Thr Lys Glu Wing Gly Gln He Wing Gly Leu Asn Val Leu Arg He 130 135 140 Val Asn Glu Pro Thr Wing Wing Wing Leu Wing Tyr Gly Leu Glu Lys Gly 145 150 155 160 Glu Gln Glu Gln Thr He Leu Val Phe Asp Leu Gly Gly Gly Thr Phe 165 170 175 Asp Val Ser Leu Leu Glu He Gly Asp Gly Val Val Glu Val Arg Ala 180 185 190 Thr Ser Gly Asp Asn Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg He 195 200 205 Val As p Trp Leu Val Glu Lys Phe Gln Ser Ser Asn Gly He Asp Leu 210 215 220 Thr Lys Asp Lys Met Wing Ala Leu Gln Arg Leu Arg Glu Wing Wing Glu Lys 225 230 235 240 Wing Lys He Glu Leu Ser Ser Ser Gln Ser Wing Asn He Asn Leu Pro 245 250 255 Tyr He Thr Val Asp Wing Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr 260 265. 270 Leu Ser Arg Wing Glu Phe Gln Arg He Thr Gln Asp Leu Leu Wing Arg 275 280 285 Thr Lys Thr Pro Phe Asn Gln Val Val Lys Asp Wing Gly Val Ser Val 290 295 300 Ser Glu He Asp His Val Val Leu Val Gly Gly Be Thr Arg Met Pro 305 310 315 320 Wing Val Thr Glu Leu Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn 325 330 335 Lys Gly Val Asn Pro Asp Glu Val Val Wing Val Gly Wing Wing Leu Gln 340 345 350 Wing Gly Val Leu Arg Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val 355 360 365 Thr Pro Leu Ser Leu Gly He Glu Thr Lys Gly Gly Val Met Thr Lys 370 375 380 Leu He Glu Arg Asn Thr Thr He Pro Thr Lys Arg Ser Glu Thr Phe 385 390 395 400 Thr Thr Ala Glu Asp Asn Gln Pro Ser Val Gln He Gln Val Phe Gln 405 410 415 Gly Glu Arg Glu He Ala Thr Ala Asn Lys Leu Leu Gly Ser Phe Glu 420 425 430 Leu Gly Gly He Ala Pro Ala Pro Arg Gly Val Pro Gln He Glu Val 435 440 445 Thr Phe Asp He Asp Wing Asn Gly He Val His Val Thr Wing Lys Asp 450 455 460 Lys Gly Thr Gly Lys Glu Asn Thr He Thr He Gln Asp Gly Ser Gly 465 470 475 480 Leu Ser Gln Asp Glu He Asp Arg Met He Lys Asp Wing Glu Wing His 485 490 495 Wing Asp Glu Asp Lys Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn 500 505 510 Wing Glu Ser Leu Val Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser 515 520 525 Glu Lys Val Ser Glu Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys 530 535 540 Gly Val Glu Glu Ala Leu Lys Gly Glu Asp Leu Glu Ala He Lys Ala 545 550 555 560 Wing Val Glu Lys Leu Asn Thr Glu Ser Gln Glu Met Gly Lys Ala He 565 570. 575 Tyr Glu Wing Asp Wing Wing Wing Gly Wing Thr Gln Wing Asp Wing Gly Wing 580 585 590 Glu Gly Wing Wing Asp Asp Asn Val Val Asp Wing Glu Val Val Glu Asp 595 600 605 Asp Wing Wing Asp Asn Gly Glu Asp Lys Lys 610 615 < 210 > 33 < 211 > 1977 < 212 > DNA < 213 > Corynebacterium glutamicum • _ < 220 > < 221 > CDS < 222 > (101) .. (1954) < 223 > FRXA02543 < 220 > < 221 > misc_feature < 222 > 1824 < 223 > n = a, t, c, or g < 220 > < 221 > misc_feature < 222 > 1830 < 223 > n = a, t, c, or g < 220 > < 221 > misc_feature < 222 > 1844 < 223 > n = a, t, c, or g < 220 > < 221 > VARIANT < 222 > 575 < 223 > Xaa = Val, Ala, Asp, or Gly < 220 > < 221 > VARIANT < 222 > 577 < 223 > Xaa = Phe, Ser, Tyr, or Cys < 220 > < 221 > VARIANT < 222 > 582 < 223 > Xaa = Ser, Pro, Thr, or Ala < 400 > 33 ctcaatgagg agtttttctt accggcgaaa gtcggtggga agcaagtcaa agctcaagcc 60 ¡Um s gtggacagta ctaaaatcac ctaaaacagg aggcaccatt atg gga cgt gca gta 115 Met Gly Arg Ala Val 1 5 gga att gac ctt gga acc acc aac tct gtg gtt tcc gta ctt gaa ggc 163 Gly He Asp Leu Gly Thr Thr Asn Ser Val Val Ser Val Leu Glu Gly 10 15 20 ggc gag cca gta gtt atc gca aac gca gaa ggc tea cgc acc action 211 Gly Glu Pro Val Val He Wing Asn Wing Glu Gly Ser Arg Thr Thr Pro 25 30 '35 tcc gtc gtt gca ttc gca aag aac ggt gaa gtt cta gtc ggc cag tcc 259 Ser Val Val Ala Phe Ala Lys Asn Gly Glu Val Leu Val Gly Gln Ser 40 45 50 gct aag aac cag gcg gtc acc aac gtt gac cgc acc att cgc tcc gtc 307 Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg Thr He Arg Ser Val 55 60 65 aag cgc cac atc ggc acc gac tgg tcc gtt gct atc gat gac aag aac 355 Lys Arg His He Gly Thr Asp Trp Ser Val Wing He Asp Asp Lys Asn 70 75 80 85 tac acc cag gaa atc tcg gct cgt acc ctg atg aag ctg aag cgc 403 Tyr Thr Ser Gln Glu He Ser Wing Arg Thr Leu Met Lys Leu Lys Arg 90 95 100 gac gct gaa gca tac cg ggc gag gac gtc act gat gct gtt att acc 451 Asp Wing Glu Wing Tyr Leu Gly Glu Asp Val Thr Asp Wing Val He Thr 105 110 115 gtt ect gca tac tc gag gac tea cag cgc cag gca acc aag gaa gct 499 Val Pro Wing Tyr Phe Glu Asp Be Gln Arg Gln Wing Thr Lys Glu Wing 120 125 130 ggt cag atc gca ggc ctt aac gtt ctg cgt att gtt aac gag cca acc 547 Gly Gln He Wing Gly Leu Asn Val Leu Arg He Val Asn Glu Pro Thr 135 140 145 gcg gct gca ctt gca tac ggc ctt gag aag ggc gag cag gag cag acc 595 Wing Wing Leu Wing Tyr Gly Leu Glu Lys Gly Glu Gln Glu Gln Thr 150 155 160 • 165 att ctg gta ttc gac ctc ggt ggc ggc acc ttc gac gtc tcc ctc cta 643 He Leu Val Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu 170 175 180 gag atc ggc gac ggt gtt gtt gag gtt cgc gca acc tcc ggc gat aac 691 Glu He Gly Asp Gly Val Val Glu Val Arg Ala Thr Ser Gly Asp Asn 185 190 195 gag ctc ggt ggc gac gac tgg gat cag cgt atc gtt gac tgg ctg gta 739 Glu Leu Gly Gly Asp Asp Trp Asp Gln Arg He Val Asp Trp Leu Val 200 205 210 gag aag ttc cag tcc tcc aac ggc att gac ctg acc aag gac aag 787 Glu Lys Phe Gln Ser Ser Asn Gly He Asp Leu Thr Lys Asp Lys Met 215 220 225 gcc ctg cag cgt ctg cgt gag gca gct gag aag gca aag atc gag ctg 835 Wing Leu Gln Arg Leu Arg Glu Wing Wing Glu Lys Wing Lys He Glu Leu 230 235 240 __ 245 tcc tct tcc cag agt gca aac atc aac ctt ect tac atc acc gtt gat 883 Being Ser Gln Ser Wing Asn He Asn Leu Pro Tyr He Thr Val Asp 250 255 260 gca gac aag aac cca ctg ttc ttg gat gag acc ctt tcc cgt gcc gag 931 Wing Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr Leu Ser Arg Ala Glu 265 270 275 ttc cag cgc atc acc cag gac ctc ctg gcc cgc acc aag act ect ttc 979 Phe Gln Arg He Thr Gln Asp Leu Leu Wing Arg Thr Lys Thr Pro Phe 280 285 290 aac cag gtt gtt aag gac gct ggc gtg tcc gtc tcg gag atc gac cac 1027 Asn Gln Val Val Lys Asp Wing Gly Val Ser Val Ser Glu He Asp His 295 300 305 gtt gtt ctc gtc ggt ggt tcc acc cgt atg ect gct gtt acc gaa ctg 1075 Val Val Leu Val Gly Gly Ser Thr Arg Met Pro Wing Val Thr Glu Leu 310 315 320 325 gtc aag gaa ctg acc ggt gga cgt gag cca aac aag ggt gtt aac cca 1123 Val Lys Glu Leu Thr Gly Arg Glu Pro Asn Lys Gly Val Asn Pro 330 335 340 gat gag gtt gtt gtt gtt ggt gca gca gca ctt cag gcc ggt gtt ctc cgc 1171 Asp Glu Val Val Wing Val Gly Wing Wing Leu Gln Wing Gly Val Leu Arg 345 350 355 ggc gag gtc aag gat gtt ctt ctt ctt gac gtc acc cca ctg tcc ctc 1219 Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu 360 365 370 ggc att gag acc aag ggt ggc gtg atg acc aag ctc atc gag cgc aac 1267 Gly He Glu Thr Lys Gly Gly Val Met Thr Lys Leu He Glu Arg Asn 375 380 385 Acc Acc Acc Acc Acc Acc Acc Acc Acc Acc Acc Acc Acc Acc Gac Gac Gac 1315 Thr Thr Thr Thr Thr Lys Arg Ser Glu Thr Thr Thr Thr Thr Thr Glu Asp 390 395 400 _ 405 aac cag ect tct gtt cag atc cag gtc cag ggc gag cgt gaa atc 1363 Asn Gln Pro Ser Val Gln He Gln Val Phe Gln Gly Glu Arg Glu He 410 415 420 gca acc gcc aac aag ctg ctc gga tcc ttc gag ctc ggc ggc atc gca 1411 Ala Thr Ala Asn Lys Leu Leu Gly Be Phe Glu Leu Gly Gly He Wing 425 430 435 ect gca cca cgt ggc gtc cca cag atc gac gtc act tcc gac atc gac 1459 Pro Wing Pro Arg Gly Val Pro Gln He Glu Val Thr Phe Asp He Asp 440 445 450 gcc aac ggc atc gtc cac gtc acc gca aag gac aag ggt act ggc aag 1507 Wing Asn Gly He Val His Val Thr Wing Lys Asp Lys Gly Thr Gly Lys 455 460 465 gaa aac acc atc acc att cag gac ggc tcc ggt ctc tcc cag gat gaa 1555 Glu Asn Thr He Thr He Gln Asp Gly Ser Gly Leu Ser Gln Asp Glu 470 475 480 485 att gat cgc atg atc aag gat gct gaa gct cac gct gat gag gac aag 1603 He Asp Arg Met He Lys Asp Ala Glu Wing His Wing Asp Glu Asp Lys 490 495 500 aag cgc cgc gag gag cag gaa gtc cgc aac aac gct gag tcc ctg gtt 1651 Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn Ala Glu Ser Leu Val 505 510 515 tac cag acc cgc aag ttc gtt gaa gag aac tcc gag aag gtc tcc gaa 1699 Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser Glu Lys Val Ser Glu 520 525 530 gac ctc aag gca aag gtc gaa gag gca gcc aag ggc gtt gaa gaa gca 1747 Asp Leu Lys Wing Lys Val Glu Glu Wing Wing Lys Gly Val Glu Glu Wing 535 540 '545 ctc aag ggc gag gac ctc gag gca atc aag gct gca gtt gag aag ctg 1795 Leu Lys Gly Glu Asp Leu Glu Ala He Lys Ala Wing Val G lu Lys Leu 550 555 560 565 aac acc gag tcc cag gaa atg ggt aag gnt atc tnc gag gct gac gct 1843 Asn Thr Glu Ser Gln Glu Met Gly Lys Xaa He Xaa Glu Wing Asp Ala 570 575 580 nct gct ggt gca acc cag gct gac gca ggt gca gaa ggc ggc gca gat 1891 Xaa Wing Gly Wing Thr Gln Wing Asp Wing Gly Wing Glu Gly Wing Wing Asp 585 590 595 gac aat gtt gtt gac gct gaa gtt gtc gaa gac gac gc gct gac aat 1939 Asp Asn Val Val Asp Ala Glu Val Val Glu Asp Asp Ala Ala Asp Asn 600 605 610 ggt gag gac aag aag taaatgacta cccctaacgg aat 1977 Gly Glu Asp Lys Lys 615 < 210 > 34 < 211 > 618 < 212 > PRT < 213 > Corynebacterium glutamicum < 220 > < 221 > VARI NT < 222 > 575 < 223 > Xaa = Val, Ala, Asp, or Gly < 220 > < 221 > VARIANT < 222 > 577 < 223 > Xaa = Phe, Ser, Tyr, or Cys < 220 > < 221 > VARIANT < 222 > 582 • < 223 > Xaa = Ser, Pro, Thr, or Ala < 400 > 34 Met Gly Arg Wing Val Gly He Asp Leu Gly Thr Thr Asn Ser Val Val 1 5 10 15 Ser Val Leu Glu Gly Gly Pro Val Val He Wing Asn Wing Glu Gly 20 25 30 Ser Arg Thr Pro Pro Ser Val Val Wing Phe Wing Lys Asn Gly Glu Val 35 40 45 10 Leu Val Gly Gln Ser Wing Lys Asn Gln Wing Val fhr Asn Val Asp Arg 50 55 60 • Thr He Arg Ser Val Lys Arg His He Gly Thr Asp Trp Ser Val Wing 65 70 75 80 He Asp Asp Lys Asn Tyr Thr Ser Gln Glu He Ser Wing Arg Thr Leu 85 90 95 Met Lys Leu Lys Arg Asp Wing Glu Wing Tyr Leu Gly Glu Asp Val Thr 100 105 110 15 Asp Wing Val He Thr Val Pro Wing Tyr Phe Glu Asp Ser Gln Arg Gln 115 120 125 Wing Thr Lys Glu Wing Gly Gln He Wing Gly Leu Asn Val Leu Arg He 130 135 140 Val Asn Glu Pro Thr Wing Wing Wing Leu Wing Tyr Gly Leu Glu Lys Gly 145 150 155 160 Glu Gln Glu Gln Thr He Leu Val Phe Asp Leu Gly Gly Gly Thr Phe 165 170 175 20 Asp Val Ser Leu Leu Glu He Gly Asp Gly Val Val Glu Val Arg Ala 180 185 190 Thr Ser Gly Asp Asn Glu Leu Gly Asp Asp Trp Asp Gln Arg He 195 200 205 Val Asp Trp Leu Val Glu Lys Phe Gln Ser Ser A'sn Gly He Asp Leu 210 215 220 Thr Lys Asp Lys Met Ala Leu Gln Arg Leu Arg Glu Ala Wing Glu Lys 225 230 235 240 25 Wing Lys He Glu Leu Being Being Gln Being Wing Asn He Asn Leu Pro 245 250 255 Tyr He Thr Val Asp Wing Asp Lys Asn Pro Leu Phe Leu Asp Glu Thr 260 265 270 Leu Ser Arg Wing Glu Phe Gln Arg He Thr Gln ASp Leu Leu Wing Arg 275 280 285 Thr Lys Thr Pro Phe Asn Gln Val Val Lys Asp Wing Gly Val Ser Val 290 295 300 Ser Glu He Asp His Val Val Leu Val Gly Gly Ser Thr Arg Met Pro 305 310 315 320 Wing Val Thr Glu Leu Val Lys Glu Leu Thr Gly Gly Arg Glu Pro Asn 325 330 335 Lys Gly Val Asn Pro Asp Glu Val Val Wing Val Gly Wing Wing Leu Gln 340 345 350 Wing Gly Val Leu Arg Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val 355 360 365 Thr Pro Leu Ser Leu Gly He Glu Thr Lys Gly Gly Val Met Thr Lys 370 375 380 Leu He Glu Arg Asn Thr Thr He Pro Thr Lys Arg Ser Glu Thr Phe 385 390 395 400 Thr Thr Ala Glu Asp Asn Gln Pro Ser Val Gln He Gln Val Phe Gln 405 410 415 Gly Glu Arg Glu He Wing Thr Wing Asn Lys Leu Leu Gly Ser Phe Glu 420 425 430 Leu Gly Gly He Wing Pro Wing Pro Arg Gly Val Pro Gln He Glu Val 435 440 445 Thr Phe Asp He Asp Wing Asn Gly He Val His Val Thr Ala Lys Asp 450 455 460 Lys Gly Thr Gly Lys Glu Asn Thr He Thr He Gln Asp Gly Ser Gly 465 470 475 480 Leu Ser Gln Asp Glu He Asp Arg Met He Lys Asp Wing Glu Wing His 485 490 495 Wing Asp Glu Asp Lys Lys Arg Arg Glu Glu Gln Glu Val Arg Asn Asn 500 505 510 Wing Glu Ser Leu Val Tyr Gln Thr Arg Lys Phe Val Glu Glu Asn Ser 515 520 525 Glu Lys Val Ser Glu Asp Leu Lys Ala Lys Val Glu Glu Ala Ala Lys 530 535 540 Gly Val Glu Glu Ala Leu Lys Gly Glu Asp Leu Glu Ala He Lys Ala 545 550 555 560 Wing Val Glu Lys Leu Asn Thr Glu Ser Gln Glu Met Gly Lys Xaa He 565 570 '575 Xaa Glu Ala Asp Ala Xaa Ala Gly Ala Thr Gln Ala Asp Ala Gly Ala 580 585 590 Glu Gly Ala Ala Asp Asp Asn Val Val Asp Ala Glu Val Val Glu Asp 595 600 605 Asp Wing Wing Asp Asn Gly Glu Asp Lys Lys 610 615 < 210 > 35 < 211 > 1947 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1924) < 223 > RXN02280 < 400 > 35 cgcgattgcg tcatcgatcg ttgttgcttc catgcgcacc acactatctt tctgcacgcc 60 ctgatgccct gtggattcaa aactgtgctt ttataggcgt atg caa gaa tcc tea 115 Met Gln Glu Ser Ser 1 5 cgt gat aat ttc caa gtt gac ctc ggc ggc gtt gtt gat ctt ttg agt 163 Arg Asp Asn Phe Gln Val Asp Leu Gly Val Val Asp Leu Leu Ser 10 15 20 cgc cac att tat tcc ggt ccg agg gtg tat gtg cgt gag ttg ctg cag 211 Arg His He Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln 25 30 35 aat gcg gtt gat gct tgt act gca cgt tct gaa cag ggt gag gag ggc 259 asn wing val asp wing cys thr wing arg be glu gln gly glu glu gly 40 45 50 tac gag ccg agt att cgt att cgg ccg gtg acc aag gat cgt gcc acg 307 Tyr Glu Pro Ser He Arg He Arg Pro Val Thr Lys Asp Arg Wing Thr 55 60 65 ttt tea ctg gtt gat aat ggt acg ggc ctg acc gcg cag gag gcg cgg 355 Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Wing Gln Glu Wing Arg 70 75 80 85 gaa ttg ctg gcg acg gtg ggg cgg acg tcg aaa cgc gat gaa ttc ggt 403 Glu Leu Leu Wing Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly 90 95 100 ctg cag cgg gaa ggt cgc ctg ggg caa ttt ggc ate ggg ctg ctt agt 451 Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly He Gly Leu Leu Ser 105 110 '_ 115 tgt ttc atg gtg gcg gat gag atc acc atg gtg tcg cat gcg gag ggt 499 Cys Phe Met Val Wing Asp Glu He Thr Met Val Ser His Wing Glu Gly 120 125 130 gcg tcg gcg att cgg tgg act ggt cat gcg gat ggc acc ttt aac ctg 547 Wing Being Wing He Arg Trp Thr Gly His Wing Asp Gl'y Thr Phe 'Asn Leu 135 140 145 gag att ctt ggg gat gat gca acg gat gtc att ccg ggc ggc acg act 595 Glu He Leu Gly Asp Asp Wing Thr Asp Val He Pro Val Gly Thr Thr 150 155 160 165 gtg cac ctg act ccg cgc ect gat gag cgc acg ttg ctg acg gaa aat 643 Val His Leu Thr Pro Arg Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn 170 175 180 tcc gtg gtc acc att gct agt aat tat ggc cgc tac ctg ccg att ect 691 Ser Val Val Thr He Ala Ser Asn Tyr Gly Arg Tyr L eu Pro He Pro 185 190 195 att gtg gtg cag ggt gag aaa aac acc acc atc act here tcg ccg gtg 739 He Val Val Gln Gly Glu Lys Asn Thr Thr Thr Thr Ser Pro Val 200 205 210 ttt gca aag gat act gat cag cag agg ctg tat gcc ggc cgg gag 787 Phe Wing Lys Asp Thr Asp Gln Gln His Arg Leu Tyr Wing Gly Arg Glu 215 220 225 cgc ctt ggt aaa act ect ttt gat gtc atc gat ctc acc ggt ect ggc 835 Arg Leu Gly Lys Thr Pro Phe Asp Val He Asp Leu Thr Gly Pro Gly 230 235 240 245 atc gag ggt gtg gct tat gta ttg cg gag gcc cag gct cgg cat atg 883 He Glu Gly Val Ala Tyr Val Leu Pro Glu Ala Gln Ala Pro His Met 250 255 260 tcc agg cgt cac agt att tat gtc aac cgc atg ttg gtc tct gat ggg 931 Ser Arg Arg His Ser He Tyr Val Asn Arg Met Leu Val Ser Asp Gly 265 270 275 ect tcc acg gtg ctg ccc aac tgg gcg ttc ttt gtg gaa tgt gaa atc 979 Pro Ser Thr Val Leu Pro Asn Trp Wing Phe Phe Val Glu Cys Glu He 280 285 290 aat tea acc gat ttg gaa ccc acc gca tcg cgt gaa gcg ctc atg gat 1027 Asn Ser Thr Asp Leu Glu Pro Thr Ala S er Arg Glu Ala Leu Met Asp 295 300 305 gac acc gcg tcc gcg gca acc agg gaa cat atc ggt gag tgc att aaa 1075 Asp Thr Wing Phe Wing Wing Thr Arg Glu His He Gly Glu Cys He Lys 310 315 320 325 tcg tgg ctg att aat ctc gcc atg acc aag ect cac cgc gtg cgg gaa 1123 Ser Trp Leu He Asn Leu Wing Met Thr Lys Pro His Arg Val Arg Glu 330 335 340 ttt act gcg att cat gat ctt gcc ctg cgc gag ctg tgc caa tcg gac 1171 Phe Thr Ala He His Asp Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp 345 350 355 gcg gac ctg gct gaa acc atg ttg ggt ctt ctc acc ttg gag acc tcc 1219 Wing Asp Leu Wing Glu Thr Met Leu Gly Leu Leu Thr Leu Glu Thr Ser 360 365 370 cgt ggt cgc atc tcg atc ggt gag atc acc acg ttg tcc atc acc gag 1267 Arg Gly Arg He Ser He Gly Glu He Thr Thr Leu Ser He Thr Glu 375 380 385 gat gtg tcg ctg cag ctg gct acc acg ttg gat gat ttc agg cag ctc 1315 Asp Val Ser Leu Gln Leu Wing Thr Thr Leu Asp Asp Phe Arg Gln Leu 390 395 400 405 aac acc att gcg cgc ccg gac acc ttg att att aat ggc ggc tac att 1363 Asn Thr He Ala Ar g Pro Asp Thr Leu He He Asn Gly Gly Tyr He 410 415 420 cac gac age gat ctg gct cgg ctc att ccc gtt cac tac cca ccg ctt 1411 His Asp Ser Asp Leu Ala Arg Leu He Pro Val His Tyr Pro Pro Leu 425 430 435 acg gta tct act gct gac ctg cgc gaa tcc atg gat ctg atg gag ctt 1459 Thr Val Ser Thr Wing Asp Leu Arg Glu Ser Met Asp Leu Met Glu Leu 440 445 450 ccg ccg cg cg gg att gg aaa gcc aag gca ctg gat gcg cag gtc 1507 Pro Pro Leu Gln Asp He Glu Lys Ala Lys Ala Leu Asp Ala Gln Val 455 460 465 acg gaa tea ttg aag gat ttt cag atc aag ggc gca acg agg gtt ttt 1555 Thr Glu Ser Leu Lys Asp Phe Gln He Lys Gly Wing Thr Arg Val Phe 470 475 480 485 gaa ccc gca gat gtt ect gcc gtg gtg atc att gat ccc aag gcg cag 1603 Glu Pro Wing Asp Val Pro Wing Val Val He He Asp Ser Lys Wing Gln 490 495 500 gcc tea cgg gat cgc aat gaa here caa age gca acc act gat cgt tgg 1651 Wing Being Arg Asp Arg Asn Glu Thr Gln Being Wing Thr Thr Asp Arg Trp 505 510 515 gct gac att ttg gca acg gtg gat aac acg ttg age cgt caa here gcc 1699 Wing Asp He Leu Wing Thr Val Asp Asn Thr Leu Ser Arg Gln Thr Wing 520 525 530 aac att cca cag gat cag gga ctg tcg gcg ttg tgc ttg aat tgg aac 1747 Asn He Pro Gln Asp Gln Gly Leu Ser Wing Leu Cys Leu Asn Trp Asn 535 540 545 aat tcg ctg gtc agg aaa ttg gcg tcc act gat gac acc gcc gtg gtg 1795 Asn Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val - ^ - ^^^ SÉ 550 555 560 '565 tcg cgc acg gtg cgt ttg ctc tac gtt cag gca ttg ttg tcc age aag 1843 Ser Arg Thr Val Arg Leu Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys 570 575 580 agg cca ctg cgg gtg aag gaa cgc gcg ctg ctt aat gat tcg ctg gca 1891 Arg Pro Leu Arg Val Lys Glu Arg Ala Leu Leu Asn Asp Ser Leu Wing 585 590 595 gat ctg gtt tct ttg tct ttg tea tcc gat atc taagacaatc ctccgctaat 1944 Asp Leu Val Ser Leu Ser Leu Ser Ser Asp He 600 605 ctt 1947 < 210 > 36 < 211 > 608 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 36 Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val 1 5 10 15 Val Asp Leu Leu Ser Arg His He Tyr Ser Gly Pro Arg Val Tyr Val 20 25 30 Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys Thr Wing Arg Ser Glu 35 40 45 Gln Gly Glu Glu Gly Tyr Glu Pro Be He Arg He Arg Pro Val Thr 50 55 60 Lys Asp Arg Wing Thr Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr 65 70 75 80 Wing Gln Glu Wing Arg Glu Leu Leu Wing Thr Val Gly Arg Thr Ser Lys 85 90 95 Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly 100 105 110 He Gly Leu Leu Ser Cys Phe Met Val Wing Asp Gl-u He Thr Met Val 115 120 125 Ser His Wing Glu Gly Wing Wing Wing Arg Trp Thr Gly His Wing Asp 130 135 140 Gly Thr Phe Asn Leu Glu He Leu Gly Asp Asp Wing Thr Asp Val He 145 150 155 160 Pro Val Gly Thr Thr Val His Leu Thr Pro Arg Pro Asp Glu Arg Thr 165 170 175 Leu Leu Thr Glu Asn Ser Val Val Thr He Wing Ser Asn Tyr Gly Arg 180 185 190 Tyr Leu Pro He Pro He Val Val Gln Gly Glu Lys Asn Thr Thr He 195 200 205 Thr Thr Ser Pro Val Phe Wing Lys Asp Thr Asp Gln Gln His Arg Leu 210 215 220 Tyr Wing Gly Arg Glu Arg Leu Gly Lys Thr Pro Phe Asp Val He Asp 225 230 235 240 Leu Thr Gly Pro Gly He Glu Gly Val Wing Tyr Val Leu Pro Glu Wing 245 250 255 Gln Ala Pro His Met Ser Arg Arg His Ser He Tyr Val Asn Arg Met 260 265 270 Leu Val Ser Asp Gly Pro Ser Thr Val Leu Pro Asn Trp Wing Phe Phe 275 280 285 Val Glu Cys Glu He Asn Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg 290 295 300 Glu Ala Leu Met Asp Asp Thr Ala Phe Ala Ala Thr Arg Glu His He 305 310 315 320 Gly Glu Cys He Lys Ser Trp Leu He Asn Leu Wing Met Thr Lys Pro 325 330 335 His Arg Val Arg Glu Phe Thr Wing He His Asp Leu Wing Leu Arg Glu 340 345 350 Leu Cys Gln Ser Asp Wing Asp Leu Wing Glu Thr Met Leu Gly Leu Leu 355 360 365 Thr Leu Glu Thr Ser Arg Gly Arg He Ser He Gly Glu He Thr Thr 370 375 380 Leu Ser He Thr Glu Asp Val Ser Leu Gln Leu Ala Thr Thr Leu Asp 385 390 • 395 400 Asp Phe Arg Gln Leu Asn Thr He Wing Arg Pro sp Thr Leu He He 405 410 415 Asn Gly Tyr He His Asp Ser Asp Leu Wing Arg Leu He Pro Val 420 425 '430 His Tyr Pro Pro Leu Thr Val Ser Thr Wing Asp Leu Arg Glu Ser Met 435 440 • 445 Asp Leu Met Glu Leu Pro Pro Leu Gln Asp He Glu Lys Wing Lys Wing 450 455 460 Leu Asp Wing Gln Val Thr Glu Ser Leu Lys Asp Phe Gln He Lys Gly 465 470 475 480 Wing Thr Arg Val Phe Glu Pro Wing Asp Val Pro Wing Val Val He He 485 490 495 Asp Ser Lys Wing Gln Wing Ser Arg Asp Arg Asn Glu Thr Gln Ser Wing 500 505 _ 510 Thr Thr Asp Arg Trp Wing Asp He Leu Wing Thr Val Asp Asn Thr Leu 515 520 525 Ser Arg Gln Thr Wing Asn He Pro Gln Asp Gln Gly Leu Ser Wing Leu 530 535 540 Cys Leu Asn Trp Asn Asn Ser Leu Val Arg Lys Leu Wing Ser Thr Asp 545 550 555 '560 Asp Thr Ala Val Val Ser Arg Thr Val Arg Leu Leu Tyr Val Gln Wing 565 570 575 Leu Leu Ser Ser Lys Arg Pro Leu Arg Val Lys Glu Arg Ala Leu Leu 580 585. 590 Asn Asp Ser Leu Wing Asp Leu Val Ser Leu Ser Leu Ser Ser Asp He 595 600 605 < 210 > 37 < 211 > 436 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (436) < 223 >; FRXA02282 < 400 > 37 cgcgattgcg tcatcgatcg ttgttgcttc catgcgcacc acactatctt tctgcacgcc 60 ctgcgcct gtggattcaa aactgtgctt ttataggcgt atg caa gaa ctc tea 115 Met Gln Glu Ser ser 1 5 cgt gat aat ttc caa gtt gac ctc ggc ggc gtt gttt gat ctt ttg agt 163 Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser 10 15 20 cgc cac att tat tcc ggt ccg agg gtg tat gtg cgt gag ttg ctg cag 211 Arg His He Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln 25 30 35 aat gcg gtt gat gct tgt act gca cgt tct gaa cag ggt gag gag ggc 259 asn wing val asp wing cys thr wing arg be glu gln gly glu glu gly 40 45 50 tac gag ccg agt att cgt att cgg ccg gtg acc aag gat cgt gcc acg 307 Tyr Glu Pro Be He Arg He Arg Pro Val Thr Lys Asp Arg Ala Thr 55 60 65 ttt tea ctg gtt gat aat ggt acg ggc ctg acc gc, g cag gag gcg cgg 355 Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Wing Gln Glu Wing Arg 70 75 80 85 gaa ttg ctg gcg acg gtg ggg cgg acg tcg aaa cgc gat gaa ttc ggt 403 Glu Leu Leu Wing Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly 90 95 100 ctg cag cgg gaa ggt cgc ctg ggg caa ttt ggc 436 Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly 105 110 < 210 > 38 < 211 > 112 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 38 Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val Asp Leu Gly Gly Val 1 5 10 15 Val Asp Leu Leu Ser Arg His He Tyr Ser Gly Pro Arg Val Tyr Val 20 25 30 Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys Thr Wing Arg Ser Glu 35 40 45 Gln Gly Glu Glu Gly Tyr Glu Pro Be He Arg He Arg Pro Val Thr 50 55 60 Lys Asp Arg Wing Thr Phe Ser Leu Val Asp Asn Gly Thr Gly Leu Thr 65 70 75 80 Wing Gln Glu Wing Arg Glu Leu Leu Wing Thr Val Gly Arg Thr Ser Lys 85 90 95 Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly 100 105 '110 < 210 > 39 < 211 > 1269 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1246) < 223 > RXA00886 < 400 > 39 taagtatgtt ggtcgtgtgc tcgctggcga atagctgcgg gtatagttgg ccatttgttt 60 tgattaatct gtttagaagc taaaggaagt atcacccacc gtg gca cgt gac tat 115 Val Ala Arg Asp Tyr 5 tac ggc att ctc ggc gtc gat cgc aat gca acc gaa tea gag atc aaa 163 Tyr Gly He Leu Gly Val Asp Arg Asn Wing Thr Glu Ser Glu He Lys 10 15 20 aag gca tac cga aag ctt gcc cgc aaa tac cac ccg gac gta aac cca 211 Lys Ala Tyr Arg Lys Leu Ala Arg Lys Tyr His Pro Asp Val Asn Pro 25 30 35 ggt gag gaa gca gcg gag aaa ttc cgc gag gct gtt gcg cat gag 259 Gly Glu Glu Glu Ala Glu Lys Phe Arg Glu Wing Ser Val Ala Glu 40 45 50 gta ctc act gat ccg gat aag cgc cgc att gtt gat atg ggc ggt gac 307 Val Leu Thr Asp Pro Asp Lys Arg Arg He Val Asp Met Gly Gly Asp 55 60 65 cca atg gag cag ggc ggc ggc gct ggc gct ggt ggc ttc ggt gga ggc 355 Pro Met Glu Gln Gly Gly Gly Gly Wing Gly Wing Gly Ghe Ply Gly Gly 70 75 80 85 ttc ggc ggc age ggt gga ctg ggc gat atc ttc gat gcc ttc ttc ggc 403 phe gly gly be gly gly leu gly asp he phe asp ala phe Phe Gly 90 95 100 ggc ggc gcg ggc ggt tcc cgt gga cca cgt tcc cgc gtg cag cca ggc 451 Gly Gly Wing Gly Gly Ser Arg Gly Pro Arg Ser Arg Val Gln Pro Gly 105 110 115 agt gac acc ttg tgg cgc acc tcc atc acc ttg gaa gag gct tac aag 499 Ser Asp Thr Leu Trp Arg Thr Ser He Thr Leu Glu Glu Wing Tyr Lys 120 125 130 ggc gct aag aaa gat ctc acc ctt gac acc gca gtg ctg tgt acc aag 547 Gly Ala Lys Lys Asp Leu Thr Leu Asp Thr Ala Val Leu Cys Thr Lys 135 140 145 tgt cat ggt tct gga tct gca tcc gac aag aag ect gtt acc tgt ggc 595 Cys His Gly Ser Gly Ser Ala Ser Asp Lys Pro Val Thr Cys Gly 150 155 160 165 acc tgt aat ggc gct ggt gaa att cag gaa gtg cag cgc age ttc ctg 643 Thr Cys Asn Gly Wing Gly Glu He Gln Glu Val Gln Arg Ser Phe Leu 170 175 180 ggc aac gtc atg acg tcc cgc cca tgc cac acc tgc gat ggc acc ggt 691 Gly Asn Val Met Thr Ser Arg Pro Cys His Thr Cys Asp Gly Thr Gly 185 190 195 gag atc cca gat ect tgc act gag tgt gca gca gat ggt cgt gtg 739 Glu He He Pro Asp Pro Cys Thr Glu Cys Ala TO the Asp Gly Arg Val 200 205 210 cgt gct cgc cgc gac atc gtg gcc aac atc cca gct ggc atc cag tcc 787 Arg Ala Arg Arg Asp He Val Wing Asn He Pro Wing Gly He Gln Ser 215 220 225 ggc atg cgc atc cgc atg gca ggc cag ggt gag gtt ggc ggt ggt ggc 835 Gly Met Arg He Arg Met Wing Gly Gln Gly Glu Val Gly Wing Gly Gly 230 235 240 245 »ggt ect gcg ggt gac ctc tac att gaa gtc atg gtg cgc ccg cac gcc 883 Gly Pro Wing Gly Asp Leu Tyr He Glu Val Met Val Arg Pro His Wing 250 255 260 atc ttc acc cgc gat ggc gac gat ctg cac gcc age atc aag gtt cca 931 He Phe Thr Arg Asp Gly Asp Asp Leu His Ala Ser He Lys Val Pro 265 270 275 atg ttc gat gca gcg ctt ggc acc gaa ttg gac gtg gaa ccc ccc acc 979 Met Phe Asp Ala Ala Leu Gly Thr Glu Leu Asp Val Glu Ser Leu Thr 280 285 290 ggc gag gag gtg aaa att acc atc ect gca ggt act cag ccc aac gat 1027 Gly Glu Glu Val Lys He Thr He Pro Wing Gly Thr Gln Pro Asn Asp 295 300 305 gtg atc acc ttg gat ggt gaa ggc atg ccg aag ctg cgc gca gaa ggc 1075 Val He Thr Leu Asp Gly Glu Gly Met Pro Lys Leu Arg Ala Glu Gly 310 315 320. 325 cac ggc aac ctc atg gcg cat gtc gat cta ttt gtg cca acc gat ttg 1123 His Gly Asn Leu Met Wing His Val Asp Leu Phe Val Pro Thr Asp Leu 330 335 340 gat gac cgc acc cgc gaa ttg ctt gaa gaa atc cgc aac cat cgc age 1171 Asp Asp Arg Thr Arg Glu Leu Leu Glu Glu He Arg Asn His Arg Ser 345 350 355 gac aac gct tcc gtg cat cgc gaa ggc gga gaa gaa tcc ggt ttc ttt 1219 Asp Asn Ala Ser Val His Arg Glu Gly Gly Glu Glu Ser Gly Phe Phe 360 365 370 gac aag ctc cga aac aag ttc cgc aaa taatgtcact gccagtattt 1266 Asp Lys Leu Arg Asn Lys Phe Arg Lys 375 380 atc - 1269 < 210 > 40 < 211 > 382 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 40 Val Ala Arg Asp Tyr Tyr Gly He Leu Gly Val Asp Arg Asn Ala Thr 1 5 10 '15 Glu Ser Glu He Lys Lys Wing Tyr Arg Lys Leu Wing Arg Lys Tyr His 20 25 30 Pro Asp Val Asn Pro Gly Glu Glu Wing Wing Glu Lys Phe Arg Glu Wing 35 40 45 Ser Val Wing His Glu Val Leu Thr Asp Pro Asp Lys Arg Arg He Val 50 55 60 Asp Met Gly Gly Asp Pro Met Glu Gln Gly Gly Gly Gly Wing Gly Wing 65 70 75 • 80 Gly Phe Gly Gly Gly Phe Gly Gly Ser Gly Gly Leu Gly Asp He Phe 85 90 95 Asp Wing Phe Phe Gly Gly Gly Wing Gly Gly Ser Arg- Gly Pro Arg Ser 100 105 110 Arg Val Gln Pro Gly Ser Asp Thr Leu Trp Arg Thr Ser He Thr Leu 115 120 125 Glu Glu Wing Tyr Lys Gly Wing Lys Lys Asp Leu Thr Leu Asp Thr Ala 130 135 140 Val Leu Cys Thr Lys Cys His Gly Ser Gly Ser Ala Ser Asp Lys Lys 145 150 155 160 Pro Val Thr Cys Gly Thr Cys Asn Gly Wing Gly Glu He Gln Glu Val 165 170 175 Gln Arg Ser Phe Leu Gly Asn Val Met Thr Ser Arg Pro Cys His Thr 180 185 190 Cys Asp Gly Thr Gly Glu He He Pro Asp Pro Cys Thr Glu Cys Wing 195 200 205 Wing Asp Gly Arg Val Arg Wing Arg Arg Asp He Val Wing Asn He Pro 210 215 220 Wing Gly He Gln Ser Gly Met Arg He Arg Met Wing Gly Gln Gly Glu 225 230 235 240 Val Gly Wing Gly Gly Gly Pro Wing Gly Asp Leu Tyr. He Glu Val Met 245 250 255 Val Arg Pro His Wing He Phe Thr Arg Asp Gly Asp Asp Leu His Wing 260 265 270 Ser He Lys Val Pro Met Phe Asp Wing Wing Leu Gly Thr Glu Leu Asp 275 280 285 Val Glu Ser Leu Thr Gly Glu Val Val Lys He Thf He Pro Wing Gly 290 295 300 Thr Gln Pro Asn Asp Val He Thr Leu Asp Gly Glu Gly Met Pro Lys 305 310 315 320 Leu Arg Ala Glu Gly His Gly Asn Leu Met Wing His Val Asp Leu Phe 325 330 335 Val Pro Thr Asp Leu Asp Asp Arg Thr Arg Glu Leu Leu Glu Glu He 340 345 350 Arg Asn His Arg Ser Asp Asn Ala Ser Val His Arg Glu Gly Gly Glu 355 360 365 Glu Ser Gly Phe Phe Asp Lys Leu Arg Asn Lys Phe Arg Lys 370 375 380 < 210 > 41 < 211 > 1470 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1447) < 223 > RXS00568 < 400 > 41 gtttcggacg acgcgagaaa tcgcattaat cgteggaaac cgggcttgtt tttgtaatat 60 ctgaaacttt ccctttcccg ateatecagg agatttaetc gtg aag agt tct gtc 115 Val Lys Ser Ser Val 1 5 gag aag ctg age gac acc cgt tea aag atc acc gtt gag gtt cca ttt 163 Glu Lys Leu Ser Asp Thr Arg Ser Lys He Thr Val Glu Val Pro Phe 10 15 20 tct gaa ctg aag cca gag atc gac cag gca tac gcc gct cta gcg cag 211 Ser Glu Leu Lys Pro Glu He Asp Gln Ala Tyr Ala Ala Leu Ala Gln 25 30 35 caa gtc cag atc ect ggt ttc cgt aag ggc aag gca ccg cgt cag ctt 259 Gln Val Gln He Pro Gly Phe Arg Lys Gly Lys Wing Pro Arg Gln Leu 40 45 50 atc gac gca cgc ttc ggc cgt ggt gcg gtt ctg gag cag gtt gtc aac 307 He Asp Wing Arg Phe Gly Arg Gly Wing Val Leu Glu Gln Val Val Asn 55 60 65 gac atg ctt ect aac cgc tac gca cag gca atc gaa gct gag ggc atc 355 Asp Met Leu Pro Asn Arg Tyr Ala Gln Ala He Glu Ala Glu Gly He 70 75 80 85 aag gca atc ggc cag ect aac gta gag gtc acc aag atc gaa gac aac 403 Lys Ala He Gly Gln Pro Asn Val Glu Val Thr Lys He Glu Asp As n 90 95 100 gag ctc gtt gag ttc gtc gct gact gtt gac gtt cgc cca gag ttc gag 451 Glu Leu Val Glu Phe Val Ala Glu Val Asp Val Arg Pro Glu Phe Glu 105 110. 115 ctt ect aag ttc gag gac atc act gtt gag gtc cca gct atc aag gct 499 Leu Pro Lys Phe Glu Asp He Thr Val Glu Val Pro Ala He Lys Wing 120 125 130 gac gaa gaca gca atc gaa gca gag ctc gag acc ctg cgt gca cgt ttc 547 Asp Glu Glu Wing He Glu Wing Glu Leu Glu Thr Leu Arg Wing Arg Phe 135 140 145 tcc acc ttg aag gat cac aac cac aag ctg aag ggt gag ttc gtc 595 Ser Thr Leu Lys Asp His Asn His Lys Leu Lys Lys Gly Glu Phe Val 150 155 160 165 acc atc aac atc acc gca age att gac ggt gag aag att gaa gag gca 643 Thr He Asn He Thr Ala Ser He Asp Gly Glu Lys He Glu Glu Wing 170 175. 180 acc act gag ggt ctg tcc tac gaa atc gga tct gac gat ctg att gac 691 Thr Thr Glu Gly Leu Ser Tyr Glu He Gly Ser Asp Asp Leu He Asp 185 190 195 ggc ctg gac aag gct ctg atc ggc gct aag aag gat gaa acc gta gag 739 Gly Leu Asp Lys Ala Leu He Gly Ala Lys Lys Asp Glu Thr Val Glu 200 205: 210 ttc acc tct gag ctg gca aac ggc gag cac aag ggc aag gaa gct caa 787 Phe Thr Ser Glu Leu Ala Asn Gly Glu His Lys Gly Lys Glu Ala Gln 215 220 225 atc age gtt gag atc acc gag acc aag cag cgc gag ctg ect gag ctg 835 He Ser Val Glu He Thr Ala Thr Lys Gln Arg Glu Leu Pro Glu Leu 230 235 240. 245 gat gat gag ttc gca cag ctg gct tct gag ttc gac acc atc gaa gag 883 Asp Asp Glu Phe Wing Gln Leu Wing Ser Glu Phe Asp Thr He Glu Glu 250 255 • 260 ctt cgt gag tcc acc gtg tct gac gtt gag gct aag cag aag aac gag 931 Leu Arg Glu Ser Thr Val Ser Asp Val Glu Wing Lys Gln Lys Asn Glu 265 270 275 cag gct gct gca atc cgc gac gaa gtt ctc gct gcg gct ctt ggc gag 979 Gln Ala Ala Ala He Arg Asp Glu Val Leu Ala Ala Ala Leu Gly Glu 280 285 • 290 gct gac ttc gct ctg cca cag tcc atc gtt gac gag cag gca cac tcc 1027 Wing Asp Phe Wing Leu Pro Gln Ser He Val Asp Glu Gln Wing His Ser 295 300 3-05 cag ctg cac cag ctc ctc ggc gag ctt gca cac gac gat gct gca ctg 1075 Gln Leu His Gln Leu Leu Gly Glu Leu Ala His Asp Asp Ala Ala Leu 310 315 320 325 aac tcc ctc ctt gag gct cag ggc acc act cgt gaa gag ttc gac aag 1123 Asn Ser Leu Leu Glu Wing Gln Gly Thr Thr Arg Glu Glu Phe Asp Lys 330 335 340 aag aac gtc gaa gat gct gag aag gct gtt cgc acc cag ctg ttc ctg 1171 Lys Asn Val Glu Asp Ala Glu Lys Ala Val Arg Th r Gln Leu Phe Leu 345 350 355 gac acc ctc tct gag gtt gag gact ect gag gtt tcc cag gag ctc 1219 Asp Thr Leu Ser Glu Val Glu Glu Pro Glu Val Ser Gln Gllu Glu Leu 360 365 '370 acc gac cac atc ctg ttc acc gca cag tct tac ggc atg gac cca aac 1267 Thr Asp His He Leu Phe Thr Wing Gln Ser Tyr Gly Met Asp Pro Asn 375 380 385 cag ttc atc ggt cag ctg cag cag tcc ggc cag atc gcg aac ctc ttc 1315 Gln Phe He Gly Gln Leu Gln Gln Ser Gly Gln He Ala Asn Leu Phe 390 395 400 405 tcc gac gtt cgc cgt ggc aag gct ctt gca cag g-ct atc tgc cgc gta 1363 Be Asp Val Arg Arg Gly Lys Ala Leu Ala Gln Ala He Cys Arg Val 410 415 420 aac gtg aag gac tcc gag ggt aac gag atc gac ect aag gaa tac tcc 1411 Asn Val Lys Asp Ser Glu Gly Asn Glu He Asp Pro Lys Glu Tyr Phe 425 430 435 ggt gaa gaa gata gta gact act gag tct gaa gct taaaaacttt 1457 Gly Glu Glu Glu Val Ala Glu Thr Glu Ser Glu Wing 440 445 aaagaaataa cgc _ 1470 < 210 > 42 < 211 > 449 < 212 > PRT < 213 > Corynebacterium glutamicum * < 400 > 42 Val Lys Being Ser Val Glu Lys Leu Being Asp Thr Arg Being Lys He Thr 1 5 10 15 Val Glu Val Pro Phe Ser Glu Leu Lys Pro Glu He Asp Gln Wing Tyr 20 25 30 Ala Ala Leu Ala Gln Gln Val Gln He Pro Gly Phe Arg Lys Gly Lys 35 40 45 Wing Pro Arg Gln Leu He Asp Wing Arg Phe Gly Arg Gly Wing Val Leu 50 55 60 Glu Gln Val Val Asn Asp Met Leu Pro Asn Arg Tyr Ala Gln Ala He 65 70 75 80 Glu Ala Glu Gly He Lys Wing He Gly Gln Pro Asn Val Glu Val Thr 85 90 95 Lys He Glu Asp Asn Glu Leu Val Glu Val Phe Val Ala Val Val Val 100 105 110 Arg Pro Glu Phe Glu Leu Pro Lys Phe Glu Asp He Thr Val Glu Val 115 120 125 Pro Wing He Lys Wing Asp Glu Glu Wing He Glu Wing Glu Leu Glu Thr 130 135 140 Leu Arg Wing Arg Phe Ser Thr Leu Lys Asp His Asn His Lys Leu Lys 145 150 155 160 Lys Gly Glu Phe Val Thr He Asn He Thr Wing Being He Asp Gly Glu 165 170 175 Lys He Glu Glu Wing Thr Thr Glu Gly Leu Ser Tyr Glu He Gly Ser 180 185 190 Asp Asp Leu He Asp Gly Leu Asp Lys Ala Leu He Gly Ala Lys Lys 195 200 205 Asp Glu Thr Val Glu Phe Thr Ser Glu Leu Ala Asn Gly Glu His Lys 210 215 220 Gly Lys Glu Ala Gln He Ser Val Glu He Thr Ala Thr Lys Gln Arg 225 230 235 240 Glu Leu Pro Glu Leu Asp Asp Glu Phe Wing Gln Leu Wing Ser Glu Phe 245 250 255 Asp Thr He Glu Glu Leu Arg Glu Ser Thr Val Ser Asp Val Glu Wing 260 265 270 Lys Gln Lys Asn Glu Gln Wing Wing Wing He Arg Asp Glu Val Leu Wing 275 280 285 Wing Wing Leu Gly Wing Wing Asp Phe Wing Leu Pro Gln Ser He Val Asp 290 295 300 Glu Gln Ala His Ser Gln Leu His Gln Leu Leu Gly Glu Leu Ala His 305 310 315 320 Asp Asp Ala Ala Leu Asn Ser Leu Leu Glu Ala Gln Gly Thr Thr Arg 325 330 - \ 335 Glu Glu Phe Asp Lys Lys Asn Val Glu Asp Wing Glu Lys Wing Val Arg 340 345 350 Thr Gln Leu Phe Leu Asp Thr Leu Ser Glu Val Glu Prolu Glu Val 355 360 365 Ser Gln Gllu Glu Leu Thr Asp His He Leu Phe Thr Wing Gln Ser Tyr 370 375 380 Gly Met Asp Pro Asn Gln Phe He Gly Gln Leu Gln Gln Ser Gly Gln 385 390. 395 400 He Ala Asn Leu Phe Ser Asp Val Arg Arg Gly Lys Ala Leu Ala Gln 405 410 415 Wing He Cys Arg Val Asn Val Lys Asp Ser Glu Gly Asn Glu He Asp 420 425 430 Pro Lys Glu Tyr Phe Gly Glu Glu Glu Val Ala Glu Thr Glu Ser Glu 435 440 445 Wing < 210 > 43 < 211 > 826 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (826) < 223 > RXN03038 < 400 > 43 gcgcggaaaa caccaagtaa gccttacagt ccgacagcct catagcggat gggataagtt 60 ccaaacacgt tcaaatccgt taaagtgcct gtttaaaact atg cat tea aag gaa 115 Met His Ser Lys Glu 1 5 gag tta here gtg cgt aaa gga att tcc cgc gtc ctc tcg gta gcg gtt 163 Glu Leu Thr Val Arg Lys Gly He Be Arg Val Leu Ser Val Ala Val 10 15 20 gct agt tea atc gga ttc gga act gta ctg here ggc acc ggc atc gca 211 Wing Being Ser He Gly Phe Gly Thr Val Leu Thr Gly Thr Gly He Wing 25 30 35 gca gct caa gac tct gca ttt gac tac ggt atg gat cca aac atg aac 259 Wing Wing Gln Asp Being Wing Phe Asp Tyr Gly Met Asp Pro Asn Met Asn 40 45 50 tac aac ccg atc gat gac atc aag gat cgt ccc gaa gga ttg tcc aat 307 Tyr Asn Pro Asp Asp Asp He Lys Asp Arg Pro Glu Gly Leu As Asn 55 60 65 ctt ccc tac ttc gga agt aaa ttg acc age tgg ggc tea tea gcc 355 Leu Pro Tyr Phe Gly Ser Lys Leu Thr Ser Trp Gly Being Tyr Ala 70 75 80 85 acc gcc tea tcc ggc gtc gtg acc tcc gcg ctc ccg cag tac acc gat 403 Thr Wing Being Ser Gly Val Val Thr Ser Ala Leu Pro Gln Tyr T hr Asp 90 95 100 ccc cgc tac ccc ctc ggc aaa gac gac ctg ccc aag gca acc atc gac 451 Pro Arg Tyr Pro Leu Gly Lys Asp Asu Leu Pro Lys Ala Thr He Asp 105 110 115 atg gag cca gaa gtt ctt gcg cgc ctt gag cga ttc gtc ggc gtt gac 499 Met Glu Pro Glu Val Leu Wing Arg Leu Glu Arg Phe Val Gly Val Asp 120 125 130, * ggt gat cgc atc cgc atc aac gcg tac tcg cca tea atg gga cgc 547 Gly Asp Arg He Arg Gln He Asn Ala Tyr Ser? 'ro Met Gly Arg 135 140 145 acc att ect cta gtc tgg gtt gtt cca gaa gac a-ac-acc gtg ect ggc 595 Thr He Pro Leu Val Trp Val Val Pro Glu Asp Asn Thr Val Pro Gly 150 155 160 165 cca acg gtc tac gca gtc gga ggc ggt gac ggt gga cag ggc ggc cag 643 Pro Thr Val Tyr Ala Leu Gly Gly Gly Asp Gly Gly Gln Gly Gly Gln 170 175 180 aac tgg gtc acc cgc acc gac ctt gag gaa tta acc agt gac aac aac 691 Asn Trp Val Thr Arg Thr Asp Leu Glu Glu Leu Thr Ser Asp Asn Asn 185 190 195 atc aac ctc atc atc ccg atg ctc gga tct ttt agt ttc tac tct gac 739 He Asn Leu He Met Pro Met Leu Gly Ser Phe Ser Phe Tyr Ser Asp 200 205 210 tgg gca cgc gaa age caa tcc atg ggt tgt gcg caa cag tgg gaa here 787 Trp Wing Arg Glu Ser Gln Ser Met Gly Cys Wing Gln Gln Trp Glu Thr 215 220 225 ttg ctc atg cac gaa ctg ect gag ccg ctt gta gcg gcc 826 Leu Leu Met His Glu Leu Pro Glu Pro Leu Val Wing Ala 230 235 240 < 210 > 44 < 211 > 242 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 44 Met His Ser Lys Glu Glu Leu Thr Val Arg Lys Gly He Ser Arg Val 1 5 10 15 Leu Ser Val Ala Wing Wing Being Ser Gly Phe Gly Thr Val Leu Thr 20 25. 30 Gly Thr Gly He Wing Wing Wing Gln Asp Wing Wing Phe Asp Tyr Gly Met 35 40 45 Asp Pro Asn Met Asn Tyr Asn Pro As Asp Asp He Lys Asp Arg Pro 50 55 60 Glu Gly Leu As Asn Leu Pro Tyr Phe Gly Ser Lys Leu Thr Ser Trp 65 70 75 80 Gly Ser Ser Tyr Ala Thr Ala Ser Ser Gly Val Val Thr Ser Ala Leu 85 90 95 Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp Leu Pro 100 105 110 Lys Wing Thr He Asp Met Glu Pro Glu Val Leu Wing Arg Leu Glu Arg 115 120 125 Phe Val Gly Val Asp Gly Asp Arg He Arg Gln He Asn Ala Tyr Ser 130 135 140 Pro Ser Met Gly Arg Thr He Pro Leu Val Trp Val Val Pro Glu Asp 145 150 155 160 Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly Asp Gly 165 170 175 Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Glu Glu Leu 180 185 190 Thr Ser Asp Asn Asn He Asn Leu He Met Met Met Leu Gly Ser Phe 195 200 205 Ser Phe Tyr Ser Asp Trp Wing Arg Glu Ser Gln Ser Met Gly Cys Wing 210 215 220 Gln Gln Trp Glu Thr Leu Leu Met His Glu Leu Pro Glu Pro Leu Val 225 230 235 240 Ala Ala < 210 > 45 < 211 > 653 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (630) < 223 > RXN03039 < 220 > < 221 > VARIANT < 222 > 141 < 223 > Xaa = Leu < 220 > < 221 > VARIANT < 222 > 142 < 223 > Xaa = Pro < 220 > < 221 > VARIANT < 222 > 166, 180 < 223 > Xaa = Ser < 220 > < 221 > VARIANT < 222 > 178 < 223 > Xaa = Tyr < 220 > < 221 > VARIANT < 222 > 195 < 223 > Xaa = Gly < 400 > 45 gca ctc ccg caa tac acc gac cca cgc tac ccc ctc ggc aaa gac gac 48 Wing Leu Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp 1 5 10 15 ctg ccc aaa gca acc atc gac atg gag cca gaa gct ctt gcg cgc ctt 96 Leu Pro Lys Wing Thr He Asp Met Glu Pro Glu Ala Leu Wing Arg Leu 20 25 30 gag cga ttc gtc ggc gtt gac ggt gat cgc atc cgc caa atc aac gcg 144 Glu Arg Phe Val Gly Val Asp Gly Asp Arg He Arg Gln He Asn Wing 35 40 45 tac tcg cca tea atg gga cgc acc att ect cta gtc tgg gtc gtg cca 192 Tyr Ser Pro Ser Met Gly Arg Thr He Pro Leu Val Trp Val Val Pro 50 55 60 gaa gac aac acc gtg ect ggc cca acg gtc tac gca ctc ggc ggc ggc 240 Glu Asp Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly 65 70 75 80 gac ggt ggc caa ggc ggc caa aac tgg gtc acc cgc acc gac ctt gat 288 Asp Gly Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Asp 85 90 95 gag ttg acc agt gaa aac aac atc aac ctc atc atc ccc atg ctc gga 336 Glu Leu Thr Ser Glu Asn Asn He Asn Leu He Met Met Pro Met Leu Gly 100 105 110 tct tt t agt ttc tac gct gac tgg gca ggc gaa age gaa tcc atg ggt 384 Ser Phe Ser Phe Tyr Wing Asp Trp Wing Gly Glu Ser Glu Ser Met Gly 115 120 125 ggt gcg caa cag tgg gaa here ttc ctc atg cac gaa ctr ccm gag ccg 432 Gly Ala Gln Gln Trp Glu Thr Phe Leu Met His Glu Xaa Xaa Glu Pro 130 135 140 cta gaa gcg gcc atc ggc gca gac ggg caa cgc age atc gtc ggc atg 480 Leu Glu Wing Wing He Gly Wing Asp Gly Gln Arg Ser He Val Gly Met 145 150 155 160 tcc atg tcc ggg gga ter gtg ctg aac ttt gcg acg cat gac ccc aac 528 Ser Met Ser Gly Gly Xaa Val Leu Asn Phe Ala Thr His Asp Pro Asn 165 170 175 ttt tay tcc tck gtc ggc tea ttt tct gga tgt gcc, gaa acc aac tcc 576 Phe Xaa Ser Xaa Val Gly Ser Phe Ser Gly Cys Wing Glu Thr Asn Ser 180 185 190 tgg atg ggr cgc cgn tgg cat cgc age cac tgc cta caa cgg caa tgt 624 Trp Met Xaa Arg Arg Trp His Arg Ser His Cys Leu Gln Arg Gln Cys 195 200 '205 cgt gcc tgagcaaatc tttggtgaag tag 653 Arg Ala 210 < 210 > 46 < 211 > 210 < 212 > PRT < 213 > Corynebacterium glutamicum < 220 > < 221 > VARIANT < 222 > 141 < 223 > Xaa = Leu < 220 > < 221 > VARIANT < 222 > 142 < 223 > Xaa = Pro < 220 > < 221 > VARIANT < 222 > 166, 180 < 223 > Xaa = Ser < 220 > < 221 > VARIANT < 222 > 178 < 223 > Xaa = Tyr < 220 > < 221 > VARIANT < 222 > 195 < 223 > Xaa = Gly < 400 > 46 Ala Leu Pro Gln Tyr Thr Asp Pro Arg Tyr Pro Leu Gly Lys Asp Asp 1 5 10 15 Leu Pro Lys Wing Thr He Asp Met Glu Pro Glu Wing Leu Wing Arg Leu 20 25 30 Glu Arg Phe Val Gly Val Asp Gly Asp Arg He Arg Gln He Asn Wing 35 40 45 Tyr Ser Pro Ser Met Gly Arg Thr He Pro Leu Val Trp Val Val Pro 50 55 60 Glu Asp Asn Thr Val Pro Gly Pro Thr Val Tyr Ala Leu Gly Gly Gly 65 70 75 80 Asp Gly Gly Gln Gly Gly Gln Asn Trp Val Thr Arg Thr Asp Leu Asp 85 90 95 Glu Leu Thr Ser Glu Asn Asn He Asn Leu He Met Pro Met Leu Gly 100 105 110 Ser Phe Ser Phe Tyr Wing Asp Trp Wing Gly Glu Ser Glu Ser Met Gly 115 120 125 Gly Wing Gln Gln Trp Glu Thr Phe Leu Met His Glu Xaa Xaa Glu Pro 130 135 140 Leu Glu Wing Wing He Gly Wing Asp Gly Gln Arg Ser He Val Gly Met 145 150 155 160 Ser Met Ser Gly Gly Xaa Val Leu Asn Phe Ala Thr His Asp Pro Asn • 165 170 175 Phe Xaa Ser Xaa Val Gly Ser Phe Ser Gly Cys Wing Glu Thr Asn Ser 180 185 190 Trp Met Xaa Arg Arg Trp His Arg Ser His Cys Leu Gln Arg Gln Cys 195 200 205 Arg Ala 210 10 < 210 > 47 < 211 > 432 < 212 > DNA • < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (409) < 223 > RXN03040 < 220 > 15 < 221 > misc_feature < 222 > 25, 108, 395 < 223 > n = a, t, c, or g < 220 > < 221 > VARIANT < 222 > 3 < 223 > Xaa = Phe, Ser, Tyr, or Cys < 220 > < 221 > VARIANT < 222 > 99 20 < 223 > Xaa = Phe, Leu, He, or Val < 400 > 47 attactctcg ctataacgat ccttntgctc aacgctgcga agctcgaaga acaagacaac 60 ctctacatct tcgccggttc cggtgtgttc tctgaactag atg tea tnc ggt gac 115 Met Ser Xaa Gly Asp 1 5 aac gca ccg att gat gag gat gcg ttc aaa aac cgc gtc ttg gtt ggg 163 Asn Ala Pro He Asp Glu Asp Ala Phe Lys Asn Arg Val Leu Val Gly 10 15 20 25 ttt gaa atc gaa gct atg tcc aac acc tgc acc cat aac ctc aag gct 211 Phe Glu He Glu Wing Met Ser Asn Thr Cys Thr His Asn Leu Lys Wing 25 30 35 gcg acc gat caa atg ggc atc gac aac atc aac tac gat ttc cga cca cc 259 wing Thr Asp Gln Met Gly He Asp Asn He Asn Tyr Asp Phe Arg Pro 40 45 50 acc gga acc accc gcc tgg gat tac tgg aac gaa gcg ctc cac cgc ttc 307 Thr Gly Thr His Wing Trp Asp Tyr Trp Asn Glu Wing Leu His'Arg Phe 55 60 65 ttc ccg ttg atg cag ggc ttc ggc ctc gac ggt ggt ccc atc ccg 355 Phe Pro Leu Met Met Gln Gly Phe Gly Leu Asp G .ly Gly Pro He Pro 70 75 80 85 atc tat aac ect aac ggt gtg acc tcc age gact tct tct nct aga act 403 He Tyr Asn Pro Asn Gly Val Thr Ser Ser Glu Ser S er Xaa Arg Thr 90 95 100 gtc ttc tgatgtgagc cttggcaccn gtg. 432 Val Phe < 210 > 48 < 211 > 103 < 212 > PRT < 213 > Corynebacterium glutamicum < 220 > < 221 > VARIANT < 222 > 3 < 223 > Xaa = Phe, Ser, Tyr, or Cys < 220 > < 221 > VARIANT < 222 > 99 < 223 > Xaa = Phe, Leu, He, or Val < 400 > 48 Met Ser Xaa Gly Asp Asn Wing Pro As As Glu Asp Wing Phe Lys Asn 1 5 10 • 15 Arg Val Leu Val Gly Phe Glu He Glu Wing Met As As Thr Cys Thr 20 25 '30 His Asn Leu Lys Ala Wing Thr Asp Gln Met Gly He Asp Asn He Asn 35 40 t 45 Tyr Asp Phe Arg Pro Thr Gly Thr His Wing Trp Asp Tyr Trp Asn Glu 50 55 '60 Wing Leu His Arg Phe Phe Pro Leu Met Met Gln £ Íy Phe Gly Leu Asp 65 70 75 80 Gly Gly Pro He Pro He Tyr Asn Pro Asn Gly Val Thr Ser Ser Glu 85 90 95 Ser Ser Xaa Arg Thr Val Phe 100 < 210 > 49 < 211 > 835 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (835) < 223 > RXN03051 < 400 > 49 acatccagaa gtagtcgttg agtatcacga gcaagtcaac gatagtaaag ataatgtcga 60 ggaactcccg ctgcctaagc gggacatagt tgcaggggac atg cgt tea gat gtt 115 Met Arg Ser Asp Val .1 5 atc gag tta ccg gag ggg gta age aag gag aaa gct gac cag cta gaa 163 He Glu Leu Pro Glu Gly Val Ser Lys Glu Lys Al-a Asp Gln Leu Glu 10 15 20 gtt gcg gaa gcg cga ctt aac gag ggt gca cga ctg atg gca acc acc 211 Val Wing Glu Wing Arg Leu Asn Glu Gly Wing Arg Leu Met Wing Thr Thr 25 30 35 ggg tgt gag gtt atg tgg cca acg ggc ttc tea gtt tgt ggc cga att 259 Gly Cys Glu Val Met Trp Pro Thr Gly Phe Ser Val. Cys Gly Arg He 40 45 50 ctt gac acc tat cgc cag gtt ggt cag ttg tea tgg ctt ggg cca 307 Leu Asp Thr Tyr Arg Gln Val Gly Gly Gln Leu Ser Trp Leu Gly Pro 55 60 65 ccg aag tea aac gag ttg acc aat ccc gac ggt gtt ggc aaa aga agt 355 Pro Lys Ser Asn Glu Leu Thr Asn Pro Asp Gly Val Gly Lys Arg Ser 70 75 80 85 gaa ttt ttt ggt gga gcc atc tat tgg cac cca gac here ggc gct tat 403 Glu Phe Phe Gly Gly Wing He Tyr Trp His Pro Asp Thr Gly Wing Tyr 90 95. 100 gca gtg acc ttg gac ggt ttg cga cag tgg ggg acc ttg aac tgg gaa 451 Wing Val Thr Leu Asp Gly Leu Arg Gln Trp Gly Thr Leu Asn Trp Glu 105 110 115 tea ggg cca ttg ggg tac cca acc tct ggt ccg atg gat here aac tat 499 Ser Gly Pro Leu Gly Tyr Pro Thr Ser Gly Pro Met Asp Thr Asn Tyr 120 125 130 ccc ctt act cag cga cag act ttt ca ggt ggt gac aac tac tac aac 547 Pro Leu Thr Gln Arg Gln Thr Phe Gln Gly Gly Asp Asn Tyr Tyr Asn 135 140 145 cca ttg act ggc ggt gct gtg tgg ggc gat att aaa cag cgc tac gaa 595 Pro Leu Thr Gly Gly Wing Val Trp Gly Asp He Lys Gln Arg Tyr Glu 150 155 160 165 gaa ctt ggc ggc tcg aat cat gcc att ggc atc ccg atc act aat gag 643 Glu Leu Gly Gly Ser Asn His Wing He Gly He Pro He Thr Asn Glu 170 175 180 cta ect age ggt act gag tat ttt tac aat aat ttc tcc aat gga here 691 Leu Pro Ser Gly Thr Glu Tyr Phe Tyr Asn Asn Phe Ser Asn Gly Thr 185 190 ^ 195 att tcg tgg cga aat gat cgt cag here cgg ttt atg. tat ttg gct acg 739 He Be Trp Arg Asn Asp Arg Gln Thr Arg Phe Met Tyr Leu Wing Thr 200 205 • 210 cag cgg gtg tgg gat gcg ttg ggt cgg gag acg ggt cgt tta ggt ttt 787 Gln Arg Val Trp Asp Ala Leu Gly Arg Glu Thr Gly Arg Leu Gly Phe 215 220 225 ect gaa gca gat gaa here ect gag gtt tct ggt cta ttc cat gtg gcg 835 Pro Glu Wing Asp Glu Thr Pro Glu Val Ser Gly Leu Phe His Val Wing 230 235 240 - 245 < 210 > 50 < 211 > 245 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 50 Met Arg Ser Asp Val He Glu Leu Pro Glu Gly Val Ser Lys Glu Lys 1 5 10 15 Wing Asp Gln Leu Glu Val Wing Glu Wing Arg Leu Asn Glu Gly Wing Arg 20 25 .. 30 Leu Met Wing Thr Thr Gly Cys Glu Val Met Trp TPro Thr Gly Phe Ser 35 40 45 Val Cys Gly Arg He Leu Asp Thr Tyr Arg Gln Val Gly Gly Gln Leu 50 55 60 Ser Trp Leu Gly Pro Pro Lys Ser Asn Glu Leu Thr Asn Pro Asp Gly 65 70 75. 80 Val Gly Lys Arg Ser Glu Phe Phe Gly Gly Wing He Tyr Trp His Pro 85 90 95 Asp Thr Gly Wing Tyr Wing Val Thr Leu Asp Gly Leu Arg Gln Trp Gly 100 105 '110 Thr Leu Asn Trp Glu Ser Gly Pro Leu Gly Tyr Pro Thr Ser Gly Pro 115 120 125 Met Asp Thr Asn Tyr Pro Leu Thr Gln Arg Gln Thr Phe Gln Gly Gly 130 135 140 Asp Asn Tyr Tyr Asn Pro Leu Thr Gly Gly Wing Val Trp Gly Asp He 145 150 155 160 Lys Gln Arg Tyr Glu Glu Leu Gly Gly Ser Asn His Wing He Gly He 165 170 175 Pro He Thr Asn Glu Leu Pro Ser Gly Thr Glu Tyr Phe Tyr'Asn Asn 180 185 190 Phe Ser Asn Gly Thr He Ser Trp Arg Asn Asp Arg Gln Thr Arg Phe 195 200 205 Met Tyr Leu Wing Thr Gln Arg Val Trp Asp Wing Leu Gly Arg Glu Thr 210 215 220 Gly Arg Leu Gly Phe Pro Glu Wing Asp Glu Thr Pro Glu Val Ser Gly 225 230 235 240 Leu Phe His Val Wing 245 < 210 > 51 < 211 > 1704 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1681) < 223 > RXN03054 < 400 > 51 ggtggaaata cgcgcacaac aattttattc acagaactta tgatttttte gggttagggt 60 cagtttgttc acatcaacta gtaacgaaag gatcatgtga atg aaa ctg ttt tcc 115 Met Lys Leu Phe Ser 1 5 aag gct gca ggc gtc att gct gca gca ctt ctt gtt gca ggt ggt ata 163 Lys Ala Ala Gly Val He Ala Ala Wing Leu Leu Val Wing Wing Gly Gly He 10 15 20 gca ect gtg gca cag ggg caa gct agt cag gtg gtc here ect gaa gac 211 Wing Pro Val Wing Gln Gly Gln Wing Wing Gln Val Val Thr Pro Glu Asp 25 30 35 caa gat gcg tat gtt caa cag ttc cac cac gaa ggg aat acc cca ect 259 Gln Asp Ala Tyr Val Gln Gln Phe His His Glu Gly Asn Thr Pro Pro 40 45 50 gtg gta gac ggg gg ggt ggc tac act gag gaa gac atc gcc gag atc 307 Val Val Asp Gly Val Gly Gly Tyr Thr Glu Gln Glu He Wing Glu He 55 60 65 cac gag gct atc cga caa gcc caa gaa tct ggc gca ect aat gaa gag 355 His Glu Ala He Arg Gln Ala Gln Glu Ser Gly Ala Pro Asn Glu Glu 70 75 80 85 ctc att ccg ggt gag atg tgg tea gat aag gtg gag ctg cca gta act 403 Leu He Pro Gly Glu Met Trp Ser Asp Lys Val Glu Leu Pro Va l Thr 90 95 100 att gat aaa gca gcc gct gat gag gca gag ata gc1 >1 att gca cag caa 451 He Asp Lys Ala Ala Ala Asp Glu Ala Glu He Ala Ala Ala Gln Gln 105 110 115 caa tct cag cca cag acg cg ggc ctt gct gcg gcg gcg tgt cag 499 Gln Ser Gln Pro Gln Thr Arg Gly Leu Wing Wing Wing Wing Wing Cys Gln 120 125 130 acg ttt tgg ccg tea ect cat cag gtt tgt ggt gct att tta gag cgc 547 Thr Phe Trp Pro Ser Pro His Gln Val Cys Gly Wing He Leu Glu Arg 135 140 145 tat att cag cag ggt gcc cag ttt ggg tgg atg ttg ttt ccg agt gaa 595 Tyr He Gln Gln Gly Wing Gln Phe Gly Trp Met Leu Phe Pro Ser Glu 150 155 160 165 ggc caa acg tta aat ect gat ggt cag ggg tat cgt cag cgg ttt atg 643 Gly Gln Thr Leu Asn Pro Asp Gly Gln Gly Tyr Arg Gln Arg Phe Met 170 175 180 aat ggg ttt gtt tat tgg cat ccg here act ggt gcg cat gct gtt aat 691 Asn Gly Phe Val Tyr Trp His Pro Thr Thr Gly Ala His Wing Val Asn 185 190 195 aat tac agt gcg cag gtg tgg gag cgt aat ggg tgg gag tct ggg tgg 739 Asn Tyr Ser Wing Gln Val Trp Glu Arg Asn Gly Trp Glu Ser Gly Trp 200 205 210 atg ggt tat ccc act ggt ggt gaa gtc ect gtg aat ggt tcc aat ccg 787 Met Gly Tyr Pro Thr Gly Gly Val Val Pro Asn Gly Ser Asn Pro 215 220 225 att gat ggt gag ttg agt ggg tgg gtg caa act ttc caa ggt ggg cga 835 He Asp Gly Glu Leu Ser Gly Trp Val Gln Thr Phe Gln Gly Gly Arg 230 235 240 '245 gtg tat cgc agt ccg gta ttg gac ggt ttc cag gtg gcc agt att aat 883 Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln Val Ala Ser He Asn 250 255 260 ggg ctg atc ttg gat aaa tgg ctt gaa ttg ggt ggt ect gat agt gac 931 Gly Leu He Leu Asp Lys Trp Leu Glu Leu Gly Gly Pro Asp Ser Asp 265 270 • 275 ctt ggt ttt ccc att gcg gat gag gct gtg here gct gac ggt gtg ggt 979 Leu Gly Phe Pr o He Wing Asp Glu Wing Val Thr Wing Asp Gly Val Gly 280 285 290 aga ttt tct gtt ttc cag aac gga gtt gtc tac tgg cat ccg caa cac 1027 Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr Trp His Pro Gln His 295 300 305 gga gct cac ect ata gta tta ggg aat ata tac agt atc tgg aga gaa gaa 1075 Gly Ala Pro His Leu Gly Asn He Tyr Ser He -Trp Arg Glu Glu 310 315 320 325 gga gct gag agt ggg gaa ttc ggt tac ect atc g, gc gat cca gaa aag 1123 Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro He Gly Asp Pro Glu Lys 330 - 335 340 tat here gaa aac atg gct aat cag gta ttc gaa aaa ggc gaa ctt gca 1171 Tyr Thr Glu Asn Met Ala Asn Gln Val Phe Glu Lys Gly Glu Leu Wing 345 350 355 gct aac cta tac ccc aat ect ctt gag gct ttt att gag ttt tta ccc 1219 Wing Asn Leu Tyr Pro Asn Pro Leu Glu Wing Phe He Glu Phe Leu Pro 360 365 '370 ttt gct aat ctt gag gaa gca ata gag tat ttt gag aac gga ttg tea 1267 Phe Wing Asn Leu Glu Glu Wing He Glu Tyr Phe Glu Asn Gly Leu Ser 375 380 385 aat tct cgt gta gag gcg aat tea ctt aac gcc aag aaa gat tcg att 1315 Asn Ser Arg Val Glu Wing Asn Ser Leu Asn Wing Lys Lys Asp Ser He 390 395 400 405 caa tgt caa tcg caa tcc gct aac att cat gtg aga acg aag agt gac 1363 Gln Cys Gln Ser Gln Ser Wing Asn He His Val Arg Thr Lys Ser Asp 410 415 420 gga gtc ggg att agg gtt cca aag att ggg ttt aag gct agg atg gat 1411 Gly Val Gly He Arg Val Pro Lys He Gly Phe Lys Ala Arg Met Asp 425 430 • 435 tgc gac ctt ect g ga act gtc tea gat gta gtg ggg tat gga tgg att 1459 Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val Gly Tyr Gly Trp He 440 445 '450 tac tac gac tat tgg gga cga tgg gg ca gca gca tat gca gca caa caa 1507 Tyr Tyr Asp Tyr Trp Gly Arg Trp Wing Gln Wing Wing Tyr Wing Gln Gln 455 460 465 ttc ttc ggt aat agg aat tct gtt gtg caa acc aat tta gag gcg ggt 1555 Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr Asn- Leu Glu Ala Gly 470 475 480. 485 tgc age ggg gag aag aat here tta ttt tgg ggt act tea tat ttt cag 1603 Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly Thr Ser Tyr Phe Gln 490 495 500 gtg act tat gaa ggt cag ccg tat ttc ggt cag tea gca act aat tac 1651 Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln Ser Wing Thr Asn Tyr 505 510 515 gct tat ctt ccg tgt acg ata gac cgt agt taacataagg aatggaatag 1701 Wing Tyr Leu Pro Cys Thr He Asp Arg Ser 520 525 gag 1704 < 210 > 52 < 211 > 527 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 52 Met Lys Leu Phe Ser Lys Wing Wing Gly Val He Wing Wing Wing Leu Leu 1 5 10 15 Val Wing Gly Wing Wing Pro Val Wing Gln Gly Gln Wing Wing Gln Val 20 25 30 Val Thr Pro Glu Asp Gln Asp Wing Tyr Val Gln Gln Phe His His Glu 35 40 45 Gly Asn Thr Pro Pro Val Val Asp Gly Val Gly Gly Tyr Thr Glu Gln 50 55 60 Glu He Wing Glu He His Glu Wing He Arg Gln Wing Gln Glu Ser Gly 65 70 75 80 Wing Pro Asn Glu Glu Leu He Pro Gly Glu Met Trp Ser Asp Lys Val 85 90 95 Glu Leu Pro Val Thr He Asp Lys Wing Wing Wing Asp Glu Wing Glu He 100 105 110 Wing He Wing Gln Gln Gln Gln Ser Gln Pro Gln Thr Arg Gly Leu Wing Wing 115 120 125 Wing Wing Wing Cys Gln Thr Phe Trp Pro Ser Pro His Gln Val Cys Gly 130 135 140 Wing He Leu Glu Arg Tyr He Gln Gln Gly Wing Gln Phe Gly Trp Met 145 150 155 160 Leu Phe Pro Ser Glu Gly Gln Thr Leu Asn Pro Asp Gly Gln Gly Tyr 165 170 - 175 Arg Gln Arg Phe Met Asn Gly Phe Val Tyr Trp His Pro Thr Thr Gly 180 185 190 Wing His Wing Val Asn Asn Tyr Ser Wing Gln Val Trp Glu Arg Asn Gly 195 200 205 T rp Glu Be Gly Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro Val 210 215 220 Asn Gly Ser Asn Pro He Asp Gly Glu Leu Ser Gly Trp Val Gln Thr 225 230 235 240 Phe Gln Gly Gly Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln 245 250 • 255 Val Ala Ser He Asn Gly Leu He Leu Asp Lys Trp Leu Glu Leu Gly 260 265 270 Gly Pro Asp Ser Asp Leu Gly Phe Pro He Ala Asp Glu Wing Val Thr 275 280 285 • Wing Asp Gly Val Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr 290 295 300 Trp His Pro Gln His Gly Wing His Pro He Leu Gly Asn He Tyr Ser 305 310 315 320 He Trp Arg Glu Glu Gly Wing Glu Ser Gly Glu Phe Gly Tyr Pro He 325 330 335 Gly Asp Pro Glu Lys Tyr Thr Glu Asn Met Wing Asn Gln Val Phe Glu 340 345 350 10 Lys Gly Glu Leu Ala Wing Asn Leu Tyr Pro Asn Pro Leu Glu Wing Phe 355 360 365 He Glu Phe Leu Pro Phe Wing Asn Leu Glu Glu Wing He Glu Tyr Phe 370 375 380 Glu Asn Gly Leu Ser Asn Ser Arg Val Glu Wing Asn Ser Leu Asn Wing 385 390 395 400 Lys Lys Asp Ser He Gln Cys Gln Ser Gln Ser Wing Asn He His Val 405 410 415 15 Arg Thr Lys Ser Asp Gly Val Gly He Arg Val Pro Lys He Gly Phe 420 425 430 Lys Ala Arg Met Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val 435 440 445 Gly Tyr Gly Trp He Tyr Tyr Asp Tyr Trp Gly Arg Trp Wing Gln Wing 450 455 460 Wing Tyr Wing Gln Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr 465 470 475 480 20 Asn Leu Glu Wing Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly 485 490 495 Thr Ser Tyr Phe Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln 500 505, • 510 Ser Wing Thr Asn Tyr Wing Tyr Leu Pro Cys Thr He Asp Arg Ser 515 520 525 < 210 > 53 25 < 211 > 456 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (433) < 223 > RXN02949 < 400 > 53 actctcgaag gttgaacaca gggctgcgat tgtgctggat caaatgtctg cacgaaaaat 60 tgttatcgcc cctggatgag tagtgattta gaggagtgct gtg age gac gag cag 115 Val Ser Asp Glu Gln 1 5 aat tct ggc gta ggc gga acg tct cgc cca acg ggt aaa cgc cag ctg 163 Asn Ser Gly Val Gly Gly Thr Ser Arg Pro Thr Gly Lys Arg Gln Leu 10 15 20 tcg ggt gct tct acct acctct tct tct tct tct tct tat gct aag cag gta 211 Ser Gly Wing Ser Thr Thr Ser Thr Ser Ser Tyr Glu Wing Lys Gln Val 25 30 35 tct here cag aag aag tea tcc ggt tcg gat tct aag ect ggc ggc ggt 259 Ser Thr Gln Lys Lys Ser Ser Gly Ser Asp Ser Lys Pro Gly Gly Gly 40 45 50 gtt att tct ttt ctg ect gag gtt gtg gga gaa gtc cgt aag gtt att 307 Val He Ser Phe Leu Pro Glu Val Val Gly Glu Val Arg Lys Val He 55 60 65 tgg ect act gcg cgc cag atg gtc acg tac acc ctt gtc gtt ttg gga 355 Trp Pro Thr Ala Arg Gln Met Val Thr Tyr Thr Leu Val Val Leu Gly 70 75 80 85 ttc ttg att gtt ttg acc gct ttg gtg tct ggt gtg gat ttc cta gct 403 Phe Leu He Val Leu Thr Ala Leu Val Ser Gly Val Asp Phe Leu Ala 90 95 100 ggt ctt gga gtt gag aag att ctg act ccg taggtaggat gtgtaacatc 453 Gly Leu Gly Val Glu Lys He Leu Thr Pro 105 110 ttt 456 < 210 > 54 < 211 > 111 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 54 Val Ser Asp Glu Gln Asn Ser Gly Val Gly Gly Thr Ser Arg Pro Thr 1 5 10 15 Gly Lys Arg Gln Leu Ser Gly Wing Ser Thr Thr Ser Thr Ser Ser Tyr 20 25 30 Glu Wing Lys Gln Val Ser Thr Gln Lys Lys Being Ser Gly Being Asp Being 35 40 45 Lys Pro Gly Gly Gly Val He Being Phe Leu Pro Glu Val Val Gly Glu 50 55 60 Val Arg Lys Val He Trp Pro Thr Wing Arg Gln Met Val Thr Tyr Thr 65 70 75 80 Leu Val Val Leu Gly Phe Leu He Val Leu Thr Ala Leu Val Ser Gly 85 90 95 Val Asp Phe Leu Ala Gly Leu Gly Val Glu Lys He Leu Thr Pro 100 105 110 < 210 > 55 < 211 > 1941 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1918) < 223 > RXN02462 < 400 > 55 tccatcctca tcgacgaagc ccgcacccca ccgggaccct ccgggaccac tagacggcac atcgcagttc tacaacgtct tcgcacagat cgtcccacgc atg acc aag gac gtt 115 Met Thr Lys Asp Val 1 5 cac tac gaa gtc gac gaa cgt aaa aag gtg gtg gtg aaa gaa gaa 163 His Tyr Glu Val Asp Glu Arg Lys Lys Thr Val Gly Val Lys Glu Glu 10 15. 20 ggc gtc gaa tac gtc gaa gac caa ctc ggc atc gac aac ctc tac gca 211 Gly Val Glu Tyr Val Glu Asp Gln Leu Gly He Asp Asn Leu Tyr Ala 25 30 35 ect gag cac tea cag ctg gtc age tac ctg aac aac gcc atc aag gca 259 Pro Glu His Ser Gln Leu Val Ser Tyr Leu Asn Asn Ala He Lys Wing 40 45 50 cag gaa ctg ttc acc cgc gac aag gac tac atc gtc cgc aac ggc gaa 307 Gln Glu Leu Phe Thr Arg Asp Lys Asp Tyr He Val Arg Asn Gly Glu 55 60 65 gtt atg atc gtc gac ggc ttc acc ggc cgt gtc ctt gcc ggc cgc cga 355 Val Met He Val Asp Gly Phe Thr Gly Arg Val Leu Wing Gly Arg Arg 70 75 80 85 tac aac gaa ggc atg cac gcg atc gaa gcc aaa gag cgc gta gag 403 Tyr Asn Glu Gly Met His Gln Wing He Glu Wing Lys Glu Arg Val Glu 90 95 • 100 atc aaa aac gag aac cag acc ctg gcg acc gtt ac ctc cag aac tac 451 He Lys Asn Glu Asn Gln Thr Leu Wing Thr Val Thr Leu Gln Asn Tyr 105 110 • 115 ttc cgc ctc tac acc aaa ctc gcc ggc atg acc ggt acc gca gag acc 499 Phe Arg Leu Tyr Thr Lys Leu Wing Gly Met Thr Gly Thr Wing Glu Thr 120 125 .130 gaa gca gaca ctc aac cag atc tac aag ctc gac gtc atc gcg atc 547 Glu Wing Wing Glu Leu Asn Gln He Tyr Lys Leu Asp Val He Wing 135 135 145 cca acc aac cga aac cag cgc gaa gac ttg acc gac ttg gtg tac 595 Pro Thr Asn Arg Pro Asn Gln Arg Glu Asp Leu Thr Asp Leu Val Tyr 150 155 160 165 aaa acca gag gct aag ttc gca gca gtc gac gac atc gca gaa 643 Lys Thr Gln Glu Ala Lys Phe Ala Ala Val Val Asp Asp He Ala Glu 170 175 180 cgc acc gaa aag ggc caa cca gtc ctc gtc ggt acc gtc tcc gtc gag 691 Arg Thr Glu Lys Gly Gln Pro Val Leu Val Gly Thr Val Ser Val Glu 185 190 195 cgc tcc gaa tac ctc tc cag ctg ttg acc aaa cga ggc atc aac cac 739 Arg Ser Glu Tyr Leu Ser Gln Leu Leu Thr Lys Arg Gly He Lys His 200 205 210 aac gtc ctc aat gcg aag cac gac cag gaa gca cag atc gtt gct 787 Asn Val Leu Asn Wing Lys His His Glu Gln Glu Wing Gln He Val Wing 215 220 225 cag gca ggt ctt cca ggc gcc gtc acc gtt gcc acc aac gcg ggc 835 Gln Wing Gly Leu Pro Gly Wing Val Thr Val Wing Thr Asn Goal the Gly 230 235 240 245 cgt gga acc gac atc gtg ctc ggc gga aac cca gaa atc ctc ctc gac 883 Arg Gly Thr Asp He Val Leu Gly Gly Asn Pro Glu He Leu Leu Asp 250 255 260 atc aaa ctc cgc gaa cgt gga ctt gat ect ttc gaa gac gaa gaa age 931 He Lys Leu Arg Glu Arg Gly Leu Asp Pro Phe Glu Asp Glu Glu Ser 265 270 275 tac cag gaa gcc tgg gac gt gaa ctt cca gca atg aag cag cga tgc 979 Tyr Gln Glu Ala Trp Asp Wing Glu Leu Pro Wing Met Lys Gln Arg Cys 280 285 290 gaa gag cgt ggc gac aaa gtc cgc gaa gcc gga gga ctc tac gtc ctt 1027 Glu Glu Arg Gly Asp Lys Val Arg Glu Wing Gly Gly Leu Tyr Val Leu 295 300 305 ggc acc gaa cgc cac gaa tcc cga cgc atc gac aac cag ctg cgc ggt 1075 Gly Thr Glu Arg His Glu Ser Arg Arg He Asp Asn Gln Leu Arg Gly 310 315 320 325 cgt tct gca cgt cag ggc gac cca gga ccc tc acc acc tcc tat ctc tct 1123 Arg Ser Wing Arg Gln Gly Asp Pro Gly Ser Thr Arg Phe Tyr Leu Ser 330 335 340 atg cgc gac gac ctg atg gtt cgc ttc gtc cgc cca acc atg gaa aac 1171 Met Arg Asp Asp Leu Met Val Arg Phe Val Gly Pro Thr Met Glu Asn 345 350 355 atg atg aac agg ctc aac gtc cca gac gat gtg ccc atc gaa tcc aaa 1219 Met Met Asn Arg Leu Asn Val Pro Asp Asp Val Pro He Glu Ser Lys 360 365 370 acc gtc acc aac tcc atc aag ggc gcc ca gct gg gg gag aac cag 1267 Thr Val Thr Asn Ser He Lys Gly Ala Gln Ala Gln Val Glu Asn Gln 375 380 385 aac ttc gaa atg cgt aag aac gtt ctg aag tac gac gaa gtc atg aac 1315 Asn Phe Glu Met Arg Lys Asn Val Leu Lys Tyr Asp Glu Val Met Asn 390 395 400 405 gaa cag cgc aag gtt atc tac age gag cga cgc gac atc ctc gaa tcc 1363 Glu Gln Arg Lys Val He Tyr Ser Glu Arg Arg Glu He Leu Glu Ser 410 415 420 gca gac atc tcc cgc tac atc caa aac atc atc gaa gaa here gtc age 1411 Wing Asp He Ser Arg Tyr He Gln Asn Met He Glu Glu Thr Val Ser 425 430"435 gca tac gtc gac ggc gcc acc gcc aac ggc tac gtc gaa gac tgg gac 1459 Wing Tyr Val Asp Gly Wing Thr Wing Asn Gly Tyr Val Glu Asp Trp Asp 440 445 450 ctc gac aaa ctc tgg aac gcc ctc gaa gcc ctc tac gac cca tcg atc 1507 Leu Asp Lys Leu Trp Asn Ala Leu Glu Ala Leu Tyr Asp Pro Ser He 455 460 465 aac tgg acc gac ctc gtc gaa ggc age gaa tac ggc aaa cca ggg gag 1555 Asn Trp Thr Asp Leu Val Glu Gly Ser Glu Tyr Gly Lys Pro Gly Glu 470 475 480 485 ctg tcc gcc gaa gat cta cgc acc gca ctc gtc aac gac gcc cac gcc 1603 Leu Ser Ala Glu Asp Leu Arg Thr Ala Leu Val A.sn Asp Ala His Wing 490 495 500 gaa tac gca aaa ctc gaa gac gcc gta tcc gca atc ggc ggc gaa gca 1651 Glu Tyr Ala Lys Leu Glu Glu Ala Val Ser Ala He Gly Gly Glu Wing 505 510 515 cag atc cgc aac atc gaa cga atg gtg ctc atg cca gtc atc gac acc 1699 Gln He Arg Asn He Glu Arg Met Val Leu Met Pro Val He Asp Thr 520 525 530 aaa tgg cgc gaa cac ctc tac gaa atg gac tac ctg aaa gaa ggc atc 1747 Lys Trp Arg Glu His Leu Tyr Glu Met Asp Tyr Leu Lys Glu Gly He 535 540 545 ggc ctg cgc gca atg gca cag cgc gac cca ctg gtc gaa tac caa aag 1795 Gly Leu Arg Ala Met Ala Gln Arg Asp Pro Leu Val Glu Tyr Gln Lys 550 555 560 565 gaa ggc ggc gac atg ttc aac ggc atg aaa gac ggc atc aag gaa gaa 1843 Glu Gly Gly Asp Met Phe Asn Gly Met Lys Asp Gly He Lys Glu Glu 570 575. 580 acc gtc cgc cag ctc ttc ctc tcc gca age agt tea tea age aag acg 1891 Thr Val Arg Gln Leu Phe Leu Being Wing Being Ser Being Being Lys Thr 585 590 595 cgg aag tcg ctg act aac tea gaa ccc tgaaattcag catccgccac 1938 Arg Lys Ser Leu Thr Asn Ser Glu Pro 600 605 atg 1941 < 210 > 56 < 211 > 606 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 56 Met Thr Lys Asp Val His Tyr Glu Val Asp Glu Arg Lys Lys Thr Val 1 5 10 15 Gly Val Lys Glu Glu Gly Val Glu Tyr Val Glu Asp Gln Leu Gly He 20 25 30 Asp Asn Leu Tyr Ala Pro Glu His Ser Gln Leu Val Ser Tyr Leu Asn 35 40 • 45 Asn Wing He Lys Wing Gln Glu Leu Phe Thr Arg Asp Lys Asp Tyr He 50 55 60 Val Arg Asn Gly Glu Val Met He Val Asp Gly Phe Thr Gly Arg Val 65 70 75 80 Leu Wing Gly Arg Arg Tyr Asn Glu Gly Met His Gln Wing He Glu Wing 85 90 95 Lys Glu Arg Val Glu He Lys Asn Glu Asn Gln Thr Leu Wing Thr Val 100 105 110 Thr Leu Gln Asn Tyr Phe Arg Leu Tyr Thr Lys Leu Wing Gly Met Thr 115 120 -, 125 Gly Thr Wing Glu Thr Glu Wing Wing Glu Leu Asn Gln He Tyr Lys Leu 130 135 140 Asp Val He Wing He Pro Thr Asn Arg Pro Asn Gln Arg Glu Asp Leu 145 150 155 •• 160 Thr Asp Leu Val Tyr Lys Thr Gln Glu Wing Lys Phe Wing Wing Val Val 165 170 175 Asp Asp He Wing Glu Arg Thr Glu Lys Gly Gln Pro Val Leu Val Gly 180 185 190 Thr Val Ser Val Glu Arg Ser Glu Tyr Leu Ser Gln Leu Leu Thr Lys 195 200 '. 205 Arg Gly He Lys His Asn Val Leu Asn Wing Lys His His Glu Gln Glu 210 215 220 Wing Gln He Val Wing Gln Wing Gly Leu Pro Gly Wing Val Thr Val Wing 225 230 235 240 Thr Asn Met Wing Gly Arg Gly Thr Asp He Val Leu Gly Gly Asn Pro 245 250 255 Glu He Leu Leu Asp He Lys Leu Arg Glu Arg Gly Leu Asp Pro Phe 260 265 270 Glu Asp Glu Glu Ser Tyr Gln Glu Wing Trp Asp Wing Glu Leu Pro Wing 275 280 285 Met Lys Gln Arg Cys Glu Glu Arg Gly Asp Lys Val Arg Glu Wing Gly 290 295 300 Gly Leu Tyr Val Leu Gly Thr Glu Arg His Glu Ser Arg Arg He Asp 305 310 315 320 Asn Gln Leu Arg Gly Arg Be Wing Arg Gln Gly Asp Pro Gly Ser Thr 325 330 335 Arg Phe Tyr Leu Ser Met Arg Asp Asp Leu Met Val Arg Phe Val Gly 340 345 350 Pro Thr Met Glu Asn Met Met Asn Arg Leu Asn Val Pro Asp Asp Val 355 360 365 Pro He Glu Ser Lys Thr Val Thr Asn Ser He Lys Gly Ala Gln Ala 370 375 380 Gln Val Glu Asn Gln Asn Phe Glu Met Arg Lys Asn Val Leu Lys Tyr 385 390 395 400 Asp Glu Val Met Asn Glu Gln Arg Lys Val He Tyr Ser Glu Arg Arg 405 410 415 Glu He Leu Glu Being Wing Asp He Being Arg Tyr He Gln Asn Met He 420 425 - 430 Glu Glu Thr Val Ser Wing Tyr Val Asp Gly Wing Thr Wing Asn Gly Tyr 435 440 445 Val Glu Asp Trp Asp Leu Asp Lys Leu Trp Asn Ala Leu Glu Ala Leu 450 455 460 Tyr Asp Pro Ser He Asn Trp Thr Asp Leu Val Glu Gly Ser Glu Tyr 465 470 475 • 480 Gly Lys Pro Gly Glu Leu Ser Wing Glu Asp Leu Arg Thr Wing Leu Val 485 490 495 Asn Asp Wing His Wing Glu Tyr Wing Lys Leu Glu Wing Wing Val Wing Wing 500 505 510 He Gly Gly Wing Wing Gln He Arg Asn He Glu Arg Met Val Leu Met 515 520 525 Pro Val He Asp Thr Lys Trp Arg Glu His Leu Tyr Glu Met Asp Tyr 530 535 540 Leu Lys Glu Gly He Gly Leu Arg Wing Met Wing Gln Arg Asp Pro Leu 545 550 555 560 Val Glu Tyr Gln Lys Glu Gly Gly Asp Met Phe Asn Gly Met Lys Asp 565 570 575 Gly He Lys Glu Glu Thr Val Arg Gln Leu Phe Leu Ser Wing Ser 580 585 590 Ser Ser Lys Thr Arg Lys Ser Leu Thr Asn Ser Glu Pro 595 600 605 < 210 > 57 < 211 > 1965 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1942) < 223 > RXN01559 < 400 > 57 gtctggttga ttggaattga aggagacttt cttggctcgg caaaaaaaga gtgccgctag 60 cgcctgggaa cgcctggaat aacgcgcaat agcgttgttt gtg ctc atc gtc gtt 115 Val Leu He Val Val 1 5 ggt gtt tat gcg ttg gtg ctg ttg here ggc gat cgt tct gcc here cca 163 Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp Arg Ser Wing Thr Pro 10 15 20 aaa ttg ggt att gat ctg ca ggc gga acc cg gtg acc ctc gtg ccg 211 Lys Leu Gly He Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val Pro 25 30 35 cag ggg cag gat cca act cag gac cag ctg aat cag gca cgc acc att 259 Gln Gly Gln Asp Pro Thr Gln Asp Gln Leu Asn Gln Wing Arg Thr He 40 45 50 ctg gaa aac cgt gtg aac ggc atg ggc gtt tea ggt gca age gtg gtc 307 Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser Gly Ala Ser Val Val 55 60 65 . "..«. «^ Gct gac ggt aac acg ctg gtg atc act gtt ccc ggg gaa aat acc gca 355 Wing Asp Gly Asn Thr Leu Val He Thr Val Pro Gly Glu Asn Thr Wing 70 75 80 85 cag gcg caa tcc cta gga cag acc tcc cag ctg ctg ttc cgt ccc gtt 403 Gln Wing Gln Ser Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro Val 90 95 100 ggt cag gca gga atg ccc gat atg acc acg ttg atg cca gag ctg gaa 451 Gly Gln Wing Gly Met Pro Asp Met Thr Thr Leu Met- Pro Glu Leu Glu 105 110 115 gag atg gcc aac agg tgg gtt gaa tac ggc gtc atc acc gaa gag cag 499 Glu Met Wing Asn Arg Trp Val Glu Tyr Gly Val He Thr Glu Glu Gln 120 125 '130 gca aat gcc tcc ttg gag gaa atg aac acc gct gt gc gc tcg acc act 547 Wing Asn Wing Ser Leu Glu Glu Met Asn Thr Ala Val Wing Ser Thr Thr 135 140 145 gcg gtg gaa ggc gaa gaa gca act gag cca gaa ccc gtc acc gtg tcg 595 Wing Val Glu Gly Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val Ser 150 155 160. 165 gcg acc ect atg gat gag cca gcc aac tcc att gag gca here cag cga 643 Thr wing Pro Met Asp Glu Pro Wing Asn Ser He Glu Wing Thr Gln Arg 170 175 •, 180 cgc cag gaa atc acg gac atg ctg cgc acc gac cgc cag tcc acc gat 691 Arg Gln Glu He Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr Asp 185 190 195 ccc act gtc cag atc gct gca agt tct tg atg cag tgc acc act gat 739 Pro Thr Val Gln He Wing Wing Being Leu Met Gln Cys Thr Thr Asp 200 205 210 gag atg gat ect ttg gcc ggc acc gat gat cca cgc ctg cca ttg gtg 787 Glu Met Asp Pro Leu Wing Gly Thr Asp Asp Pro Arg Leu Pro Leu Val 215 220 225 gca tgt gat cca gct gta ggt ggt gtg tat gta ctt gat ect gca ect 835 Wing Cys Asp Pro Wing Val Gly Gly Val Tyr Val Leu Asp Pro Wing Pro 230 235 240 245 ttg ctc aac ggc gaa gat gag gaa aat ggt gcg cgc cta acc ggt 883 Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly Wing Arg Leu Thr Gly 250 255 260 aat gag atc gat acc aac cgt ccc atc acc ggt gga ttc aac gcc cag 931 Asn Glu He Asp Thr Asn Arg Pro He Thr Gly Gly Phe Asn Wing Gln 265 270 275 tcc ggc cag atg gaa atc age ttt gcc ttc aaa tcc ggc gat ggg gaa 979 Ser Gly Gln Met Glu He Ser Phe Ala Phe Lys Ser Gly Asp Gly Glu 280 285 290 gaa ggc tct gca act tgg tcc tct tct ctg acc age cag tac cg cag cag 1027 Glu Gly Ser Wing Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln Gln 295 300 305 cag atc gcc atc acc ctg gac tct cag gtg att tct gca ccc gtg att 1075 Gln He Wing He Thr Leu Asp Ser Gln Val He Ser Wing Pro Val He 310 315 320. 325 cag tea gca acc ect gtg ggt tct gca here tcc atc acc ggt gac ttc 1123 Gln Ser Wing Thr Pro Val Gly Ser Wing Thr Ser He Thr Gly Asp Phe 330 335 340 act caa act gaa gcc caa gat ctg gcg aac aac ctg cgc tac ggt gca 1171 Thr Gln Thr Glu Wing Gln Asp Leu Wing Asn Asn Leu Arg Tyr Gly Wing 345 350 355 ttg ccc ctg age ttc gca ggt gaa aac ggc gag cgc ggc gga act acc 1219 Leu Pro Leu Ser Phe Wing Gly Glu Asn Gly Glu Arg Gly Gly Thr Thr 360 365 370 acc acc gtt ccg cca gcc cca te gca gca gcc tcc ttg aag gcc gga ctg 1267 Thr Thr Val Pro Pro Ser Leu Gly Ala Wing Ser Leu Lys Wing Gly Leu 375 380 385 atc gca ggc atc gtc ggc atc gcg ctg gtc gcc atc ttc gtg tcc gcc 1315 He Ala Gly He Val Gly He Ala Leu Val Ala He Phe Val Phe Wing 390 395 400 405 tac tac cgc gtc ttc gga ttc gtt tcc ctg ttc acc ctg ttt gcc gca 1363 Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Wing Wing 410 415 420 gg gtg gtc gtc gtc gtc gtc gt ctg gt ctg cgg gg cgc tgg atg gga 1411 Gly Val Leu Val Tyr Gly Leu Leu Val Leu Glu Arg Leu Trp He Gly 425 430 435 tat tcc cta gac ctt gct ggt atc gcc gt ttg atc atc ggt atc ggt 1459 Tyr Ser Leu Asp Leu Wing Gly He Wing Gly Leu He He Gly Gly He 440 445 450 acc acc gcc gac tcc ttc gtg gtg ttc tat gag cgc atc aag gat gag 1507 Thr Thr Wing Asp Ser Phe Val Val Phe Tyr Glu Arg He Lys Asp Glu 455 460 465 ate cgt gaa gga aga tcc ttt aga tct gca gta ect cgt gca tgg gaa 1555 He Arg Glu Gly Arg Be Phe Arg Be Wing Val Pro Arg Wing Trp Glu 470 475 480 485 age gcc aag cgc acc atc gtc here ggc aac atg gtc act ttg ctc ggc 1603 Be Ala Lys Arg Thr He Val Thr Gly Asn Met Val Thr Leu Leu Gly 490 495 500 gct atc gtg att tac ttg ctc gcg gtc ggc gaa gtc aag ggc ttt gcc 1651 Ala He Val He Tyr Leu Leu Ala Val Gly Glu Val Lys Gly Phe Ala 505 510 515 ttc acc ctg ggt ctg acc acc gta ttc gat ctc gtt gtc acc ttc ctg 1699 Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe Leu 520 525 530 atc acg gca cca ctg gtt atc ctg gca tea cgc aac cca ttc ttt gcc 1747 He Thr Ala Pro Leu Val He Leu Ala S er Arg Asn Pro Phe Phe Wing 535 540 545 aag tea tcg gtc aac ggc atg gga cga gtg atg aag ctc gtt gaa gaa 1795 Lys Ser Ser Val Asn Gly Met Gly Arg Val Met Lys Leu Val Glu Glu 550 555 560 565 cgc cgc gcc aac ggt gaa ttg gat gag ect gag tac ctg aaa aag atc 1843 Arg Arg Wing Asn Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys He 570 575 580 cat gcc aag aat gcg gca gct gat aag gct tcc act gac aat tct tcc 1891 His Wing Lys Asn Wing Wing Wing Asp Lys Wing Ser Thr Asp Asn Ser Ser 585 590 595 act gac aat tct gaa gca ect ggc acc ga gag gag gag 1939 Thr Asp Asn Ser Glu Wing Pro Gly Thr Asp Thr Asn Gln Glu Glu Glu 600 605 610 aag tagccatgac tgattcccag act 1965 Lys < 210 > 58 < 211 > 614 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 58 Val Leu He Val Val Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp 1 5 10 15 Arg Be Wing Thr Pro Lys Leu Gly He Asp Leu Gln Gly Gly Thr Arg 20 25 -. 30 Val Thr Leu Val Pro Gln Gly Gln Asp Pro Thr, Gln Asp Gln Leu Asn 35 40 45 Gln Ala Arg Thr He Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser 50 55 60 Gly Ala Ser Val Val Ala Asp Gly Asn Thr Leu Val He Thr Val Pro 65 70 75 80 Gly Glu Asn Thr Ala Gln Ala Gln Ser Leu Gly Gln Thr Ser Gln Leu 85 90 95 Leu Phe Arg Pro Val Gly Gln Ala Gly Met Pro Asp Met Thr Thr Leu 100 105 110 Met Pro Glu Leu Glu Glu Met Wing Asn Arg Trp Val Glu Tyr Gly Val 115 120 125 He Thr Glu Glu Gln Wing Asn Wing Being Leu Glu Glu Met Asn Thr Wing 130 135 140 Val Wing Ser Thr Thr Wing Val Glu Gly Glu Glu Wing Thr Glu Pro Glu 145 150 155 160 Pro Val Thr Val Ser Wing Thr Pro Met Asp Glu Pro Wing Asn Ser He 165 170 '175 Glu Ala Thr Gln Arg Arg Gln Glu He Thr Asp Met Leu Arg Thr Asp 180 185 190 Arg Gln Ser Thr Asp Pro Thr Val Gln He Ala Wing Ser Leu Met 195 200 205 Gln Cys Thr Thr Asp Glu Met Asp Pro Leu Ala Gly Thr Asp Asp Pro 210 215 220 Arg Leu Pro Leu Val Wing Cys Asp Pro Wing Val Gly Gly Val Tyr Val 225 230 235 240 Leu Asp Pro Pro Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly 245 250 255 Wing Arg Leu Thr Gly Asn Glu He Asp Thr Asn Arg Pro He Thr Gly 260 265 • 270 Gly Phe Asn Wing Gln Ser Gly Gln Met Glu He Ser Phe Wing Phe Lys 275 280 285 Ser Gly Asp Gly Glu Glu Gly Ser Wing Thr Trp Ser Ser Leu Thr Ser 290 295 300 Gln Tyr Leu Gln Gln Gln He Wing He Thr Leu Asp Ser Gln Val He 305 310 315 320 Be Ala Pro Val He Gln Be Ala Thr Pro Val Gly Ser Ala Thr Ser 325 330 335 He Thr Gly Asp Phe Thr Gln Thr Glu Wing Gln Asp Leu Wing Asn Asn 340 345 350 Leu Arg Tyr Gly Wing Leu Pro Leu Being Phe Wing Gly Glu Asn Gly Glu 355 360 365 Arg Gly Gly Thr Thr Thr Thr Val Pro Pro Ser Leu Gly Ala Ala Ser 370 375 380 'Leu Lys Ala Gly Leu He Ala Gly He Val Gly He Ala Leu Val Ala 385 390 395 400 He Phe Val Phe Wing Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe 405 410 415 Thr Leu Phe Wing Wing Gly Val Leu Val Tyr Gly Leu Leu Val Leu Leu 420 425 430 Gly Arg Trp He Gly Tyr Ser Leu Asp Leu Wing Gly He Wing Gly Leu 435 440"445 He He Gly He Gly Thr Thr Wing Asp Being Phe Val Val Phe Tyr Glu 450 455 460 Arg He Lys Asp Glu He Arg Glu Gly Arg Ser Phe Arg Ser Wing Val 465 470 475 480 Pro Arg Wing Trp Glu Be Wing Lys Arg Thr He Val Thr Gly Asn Met 485 490 495 Val Thr Leu Leu Gly Wing He Val He Tyr Leu Leu Wing Val Gly Glu 500 505 510 Val Lys Gly Phe Wing Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu 515 520 525 Val Val Thr Phe Leu He Thr Wing Pro Leu Val He Leu Wing Ser Arg 530 535 540 Asn Pro Phe Phe Wing Lys Ser Val Valid Asn Gly Met Gly Arg Val Met 545 550 555 560 Lys Leu Val Glu Glu Arg Arg Wing Asn Gly Glu Leu Asp Glu Pro Glu 565 570 575 Tyr Leu Lys Lys He His Wing Lys Asn Wing Wing Wing Asp Lys Wing Ser 580 585 590 Thr Asp Asn Ser Ser Thr Asp Asn Ser Glu Wing Pro Gly Thr Asp Thr 595 600 605 Asn Gln Glu Glu Glu Lys 610 < 210 > 59 < 211 > 819 < 212 > DNA i < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (796) < 223 > RXN00046 < 400 > 59 tggtgcccaa gcagccgtca tcgcagcagc aaaatatgcc cgcgataacg ccttttaagc 60 acctaaaacg ctgttctcaa cacaggagtt tccttaaata atg gac tta aat act 115 Met Asp Leu Asn Thr 1 5 caa cgc tea aag ctc tac gca cag ctt caa ggc cag ctc att gtt tcc 163 Gln Arg Ser Lys Leu Tyr Ala Gln Leu Gln Gly Gl'n Leu He Val Ser 10 15 20 gtg caa gct ccc gac ggc cat gcc atg cga gat acc acc ac ctc 211 Val Gln Ala Pro Asp Gly His Ala Met Arg Asp Thr His Thr Leu Thr 25 30 35 cat gtg gcc gca gcc gtg tgt ggc ggc gct gct gct gcc cgc att tgt 259 His Val Ala Wing Wing Cys Val Asp Gly Wing Ala Pro Wing He Arg Cys 40 45 50 ggt ggt ggt ggt ggt gt gtt gtt gat atc cgt tea atc tcc aac cgt gtc 307 Gly Gly Tyr Gly Gly Leu Glu Asp He Arg Be He Being Asn Arg Val 55 60 65 gac gtt ccc gtt ttc gga ctc acc aaa gaa ggc tcc gaa gga gtt tac 355 Asp Val Pro Val Phe Gly Leu Thr Lys Glu Gly Ser Glu Val Tyr 70 75 80 85 atc acc cca acc agg gat tcc gtt cga gca gtg gca gaa tcc ggc gcc 403 He Thr Pro Thr Arg Asp Ser Val Arg Ala Val Ala Glu Ser Gly Wing 90 95 100 act gta gtc gcg gcg gat gca act ttc cga ect agg ect gac ggc tcc 451 Thr Val Val Cys Wing Asp Ala Thr Phe Arg Pro Arg Pro Asp Gly Ser 105 110 115 acc ttt gca gag ctg gtc act gtt gcc cac gat tcc gga att ctc atc 499 Thr Phe Wing Glu Leu Val Thr Val Wing His Asp Ser Gly He Leu He 120 125 '130 atg gcg gac tgc gca act ccc gaa gaa gtt ctc agt gcg cat aag gct 547 Met Wing Asp Cys Wing Thr Pro Glu Glu Val Leu Ser Wing His Lys Wing 135 140 145 ggc gcg gat ttt gtg tcc acc acg ctt gct gga tac acc gaa cac cgc 595 Gly Wing Asp Phe Val Ser Thr Thr Leu Wing Gly Tyr Thr Glu His Arg 150 155 160 165 gag aaa here gtc ggt cca gat ttc gat tgc ctc cgc gaa gca cgt gag 643 Glu Lys Thr Val Gly Pro Asp Phe Asp Cys Leu Arg Glu Al to Arg Glu 170 175 180 tta gtt ccc gat gcg ttc ctc att ggc gaa ggt cgc ttc tcc aac ect 691 Leu Val Pro Asp Wing Phe Leu He Gly Glu Gly Arg Phe Ser Asn Pro 185 190 195 gcg gat gtg gcg cac ggt cgt ctc att ggt gcc aac gcg atc atc gtg 739 Wing Asp Val Wing His Gly Arg Leu He Gly Wing Asn Wing He He Val 200 205 210 ggc acc gca atc act gac ect ggt ttc atc act gga cag ttc gcg tea 787 Gly Thr Ala He Thr Asp Pro Gly Phe He Thr Gly Gln Phe Wing Ser 215 220 225 ctg ttg cac tageaettag tccagcgctg cac 819 Leu Leu His 230 < 210 > 60 < 211 > 232 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 60 Met Asp Leu Asn Thr Gln Arg Ser Lys Leu Tyr Wing Gln Leu Gln Gly 1 5 10 15 Gln Leu He Val Val Gln Ala Pro Asp Gly His Ala Met Arg Asp 20 25 30 Thr His Thr Leu Thr His Val Ala Ala Ala Cys Val Asp Gly Gly Ala 35 40 45 Pro Ala He Arg Cys Gly Gly Tyr Gly Glu Leu Glu Asp He Arg Ser 50 55 60 He Ser Asn Arg Val Asp Val Pro Val Phe Gly Leu Thr Lys Glu Gly 65 70 75 80 Ser Glu Gly Val Tyr He Thr Pro Thr Arg Asp Ser Val Arg Ala Val 85 90 95 Wing Glu Ser Gly Wing Thr Val Val Cys Wing Asp Wing Thr Phe Arg Pro 100 105 110 Arg Pro Asp Gly Ser Thr Phe Wing Glu Leu Val Thr Val Wing His Asp 115 120 125 Ser Gly He Leu He Met Wing Asp Cys Wing Thr Pro Glu Glu Val Leu 130 135 140 Ser Ala His Lys Ala Gly Ala Asp Phe Val Ser Thr Thr Leu Ala Gly 145 150 155 160 Tyr Thr Glu His Arg Glu Lys Thr Val Gly Pro Asp Phe Asp Cys Leu 165 170 175 Arg Glu Wing Arg Glu Leu Val Pro Asp Wing Phe Leu He Gly Glu Gly 180 185 190 Arg Phe Ser Asn Pro Wing Asp Val Wing His Gly Arg Leu He Gly Wing 195 200 205 Asn Ala He He Val Gly Thr Ala He Thr Asp Pro Gly Phe He Thr 210 215 220 Gly Gln Phe Ala Ser Leu Leu His 225 230 < 210 > 61 < 211 > 1219 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (48) .. (1196) < 223 > RXN01863 < 400 > 61 ggtatcatac cgatatgaac caaatagaaa gaaggaagtt taagacg atg aat age 56 Met Asn Be 1 gtc aaa ttg aag caa ect gtt age att tac aat gat cca tgg gaa tea 104 Val Lys Leu Lys Gln Pro Val Ser He Tyr Asn Asp Pro Trp Glu Ser 5 10 ' 15 tat aac gat gtt aaa gaa cat ggc caa tta act tta agt aac atc gaa 152 Tyr Asn Asp Val Lys Glu His Gly Gln Leu Thr Leu Ser Asn He Glu 20 25 30 35 ttt here act aat ctt tgt aat atg cgt tgt age cac tgt gca gtt 200 Phe Thr Thr Asn Leu Cys Asn Met Arg Cys Ser His Cys Ala Val 40 45 50 ggt tat act tta caa act gtc gac ccc gag ect tta gat atg gac tta 248 Gly Tyr Thr Leu Gln Thr Val Asp Pro Glu Pro Leu Asp Met Asp Leu 55 60 65 att tat cgt aga ctt gat gaa att cca aat ctg cg a acg atg tea att 296 He Tyr Arg Arg Leu Asp Glu He Pro Asn Leu Arg Thr Met Ser He 70 75 80 here ggt ggc gaa cca atg ttt tct aaa aag tct att aga aat gtt gtt 344 Thr Gly Gly Pro Met Phe Ser Lys Lys Ser He Arg Asn Val Val 85 90 95 aaa ect cta tta aag tat gca cat cat cga ggt ata tat here caa a tg 392 Lys Pro Leu Leu Lys Tyr Ala His His Arg Gly He Tyr Thr Gln Met 100 105 110 115 aat tea aac cta here ttg ect caa gat cta tat tta gat att gct gaa 440 Asn Ser Asn Leu Thr Leu Pro Gln Asp Arg Tyr Leu Asp He Wing Glu 120 125 130 tat atc gat gtt atg cat atc tea cat aac tgg gga here act gat gaa 488 Tyr He Asp Val Met His He Ser His Asn Trp Gly Thr Thr Asp Glu 135 140 145 ttc gca aat gtt ggc ttt ggc gca atg aag aag caa cca ccg tta aaa 536 Phe Ala Asn Val Gly Phe Gly Ala Met Lys Lys Gln Pro Pro Leu Lys 150 155 160 gct aag tta aaa tta tat gaa caa atg att tcg aat gca cgt here tta 584 Ala Lys Leu Lys Leu Tyr Glu Gln Met He Be Asn Wing Arg Thr Leu 165 170 175 tea gaa caa gga atg ttt gta tct gcg gaa here atg ctc aat caa agt 632 Ser Glu Gln Gly Met Phe Val Ser Wing Glu Thr Met Leu Asn Gln Ser 180 185 190 195 acg cta cca cat tta cga aaa ata cat caa gaa gtc gtt cat gat atg 680 Thr Leu Pro His Leu Arg Lys He His Gln Glu Val Val His Asp Met 200 205 210 aaa tgt age aga cac gag att cac ect at g tat cca gct gc gac ttt gca 728 5 Lys Cys Ser Arg His Glu He His Pro Met Tyr Pro Wing Asp Phe Wing 215 220 225 agt caa tta aat gtg tta act cta gcg gaa atg aaa aag here att 776 Ser Gln Leu Asn Val Leu Thr Leu Wing Glu Met Lys Lys Thr He His 230 235 240 gat ata ttg gat ttc gat gat gat gat tg atg tta ttt ggt act 824 Asp He Leu Asp Phe Arg Asp Glu Asp He Trp Met Leu Phe Gly Thr 245 250 255 ttg ect gtg ttt cca tgc tta aag gat gat gaa gat caa aag tta cta 872 10 Leu Pro Val Phe Pro Cys Leu Lys Asp Asp Glu Asp Gln Lys Leu Leu 260 265 270. 275 tea cgt tta aga aat gct aac aat gta acg act aga gac ccg gat 920 Ser Arg Leu Arg Asn Ala Asn Asn Val Thr Thr Arg Asn Asp Pro Asp • 280 285 290 ggc cgt agt cgt tta aat gtc aat gta ttt here ggt aat gta atc gta 968 Gly Arg Ser Arg Leu Asn Val Asn Val Phe Thr Gly Asn Val He Val 295 300, 305 act gat ttc gga gat gaa here ggt here att tcg aat ata aaa gat 1016 15 Thr Asp Phe Gly Asp Glu Thr Gly Thr He Ser Asn He Gln Lys Asp 310 315 320 aaa tta here gat gta ttt gat aaa tgg tta tcc tct gat ctt gct aaa 1064 Lys Leu Thr Asp Val Phe Asp Lys Trp Leu Ser Ser Asp Leu Ala Lys 325 330 335 tea tta aat tgt tgt cat tg ttt tt agt tgt tta gga cca aat gtt 1112 Ser Leu Asn Cys His Cys Ser Glu Phe Ser Cys Leu Gly Pro Asn Val • 340 345 350 355 ctt gtt aaa atat tac tat ccg atat gat ttt aaa gat aat gag 1160 20 Leu Val Lys Asn Met Tyr Tyr Pro Asn Met Asp Phe Lys Asp Asn Glu 360 365 370 cgt cat atg cac aaa caa cca caa att ata caa ttt taaaaactct 1206 Arg His Met His Lys Gln Pro Gln He He Gln Phe 375 380 taattatgcg gag 1219 < 210 > 62 < 211 > 383 25 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 62 Met Asn Ser Val Lys Leu Lys Gln Pro Val Ser He Tyr Asn Asp Pro 1 5 10 15 Trp Glu Ser Tyr Asn Asp Val Lys Glu His Gly Gln Leu Thr Leu Ser 20 25 30 Asn He Glu Phe Thr Thr Asn Leu Cys Asn Met Arg Cys Ser His 35 40 45 Cys Ala Val Gly Tyr Thr Leu Gln Thr Val Asp Pro Glu Pro Leu Asp 50 55 60 Met Asp Leu He Tyr Arg Arg Leu Asp Glu He Pro Asn Leu Arg Thr 65 70 75 80 Met Be He Thr Gly Gly Glu Pro Met Phe Ser Lys Lys Ser He Arg 85 90 95 Asn Val Val Lys Pro Leu Leu Lys Tyr Ala His His Arg Gly He Tyr 100 105 110 Thr Gln Met Asn Ser Asn Leu Thr Leu Pro Gln Asp Arg Tyr Leu Asp 115 120 125 He Wing Glu Tyr He Asp Val Met His He Ser His Asn Trp Gly Thr 130 135 140 Thr Asp Glu Phe Wing Asn Val Gly Phe Gly Wing Mét Lys Lys Gln Pro 145 150 155 160 Pro Leu Lys Ala Lys Leu Lys Leu Tyr Glu Gln Met He Ser Asn Ala 165 170 175 Arg Thr Leu Ser Glu Gln Gly Met Phe Val Ser Wing Glu Thr Met Leu 180 185 190 Asn Gln Ser Thr Leu Pro His Leu Arg Lys He Hs Gln Glu Val Val 195 200 205 His Asp Met Lys Cys Ser Arg His Glu He His Pro Met Tyr Pro Wing 210 215 220 Asp Phe Wing Being Gln Leu Asn Val Leu Thr Leu Wing Glu Met Lys Lys 225 230 235 240 Thr He His Asp He Leu Asp Phe Arg Asp Glu Aßp He Trp Met Leu 245 250 255 Phe Gly Thr Leu Pro Val Phe Pro Cys Leu Lys Asp Asp Glu Asp Gln 260 265 270 Lys Leu Leu Ser Arg Leu Arg Asn Wing Asn Val Thr Thr Arg Asn 275 280 285 Asp Pro Asp Gly Arg Ser Arg Leu Asn Val Asn Val Phe Thr Gly Asn 290 295 300 Val He Val Thr Asp Phe Gly Asp Glu Thr Gly Thr He Ser Asn He 305 310 315 320 Gln Lys Asp Lys Leu Thr Asp Val Phe Asp Lys Trp Leu Ser Ser Asp 325 330 335 Leu Ala Lys Ser Leu Asn Cys His Cys Ser Glu Phe Ser Cys Leu Gly 340 345 350 Pro Asn Val Leu Val Lys Asn Met Tyr Tyr Pro Asn Met Asp Phe Lys 355 360 365 Asp Asn Glu Arg His Met His Lys Gln Pro Gln He He Gln Phe 370 375 380 < 210 > 63 < 211 > 618 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (595) < 223 > RXN00833 < 400 > 63 agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct 60 tttctagcac caaaacaaaa ctctccctag tatggggtcc atg gct aaa here cat 115 Met Ala Lys Thr His 1 5 ttt caa ggc aac gaa act gct acc tcc ggc gaa ctg cca cag gtc ggc 163 Phe Gln Gly Asn Glu Thr Ala Thr Be Gly Glu Leu Pro Gln Val Gly 10 15 20 gac aac ctc gca gag ttc aac ctc gtc aac acc gaa ctg ggc gag gtc 211 Asp Asn Leu Wing Glu Phe Asn Leu Val Asn Thr Glu Leu Gly Glu Val 25 30 35 tcc tea aag gac ttc cag ggc cgc aag ctt gtc ctg aac atc ttc cca 259 Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val Leu Asn He Phe Pro 40 45 50 tcc gtt gac acc ggc gtt tgt gca here tea gtc cgc aag ttc aac gag 307 Ser Val Asp Thr Gly Val Cys Wing Thr Ser Val Arg Lys Phe Asn Glu 55 60 65 gca gca gca age ctg gaa aac acc acc gtg ctg tgc atc tcc aag gat 355 Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu Cys He Ser Lys Asp 70 75 80 85 ctt cca ct cca gtc ggc cgt ct tc tcc gca gaca ggc atc gag aac 403 Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala Glu Gly H e Glu Asn 90 95 100 gtc acc cca gta tcc gca ttc cgt tcc acc ttc ggt gaa gac aac ggc 451 Val Thr Pro Val Ser Wing Phe Arg Ser Thr Phe Gly Glu Asp Asn Gly 105 110 115 atc gtg ctc gaa ggc tea cca ctt aag ggt ctt ctt gca cgc age gtc 499 He Val Leu Glu Gly Ser Pro Leu Lys Gly Leu Leu Wing Arg Ser Val 120 125 130 atc gtc gtc gat gaa aac ggc aag gtt gct tac acc cag ttg gtt gat 547 He Val Val Asp Glu Asn Gly Lys Val Wing Tyr Thr Gln Leu Val Asp 135 140 145 gag atc ttc act gaa ect gat tac gac gct gca ctt gct ggg ctg aac 595 Glu He Phe Thr Glu Pro Asp Tyr Asp Ala Ala Leu Ala Gly Leu Asn 150 155 160 165 taatttactt cgctcagggg aat 618 < 210 > 64 < 211 > 165 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 64 Met Wing Lys Thr His Phe Gln Gly Asn Glu Thr Wing Thr Ser Gly Glu 1 5 10 15 Leu Pro Gln Val Gly Asp Asn Leu Wing Glu Phe Asn Leu Val Asn Thr 20 25 30 Glu Leu Gly Glu Val Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val 35 - 40 45 Leu Asn He Phe Pro Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val 50 55 60 Arg Lys Phe Asn Glu Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu 65 70 75 80 Cys He Ser Lys Asp Leu Pro Phe Wing Leu Gly Arg Phe Cys Ser Wing 85 90% 95 Glu Gly He Glu Asn Val Thr Pro Val Ser Wing Phe Arg Ser Thr Phe 100 105 110 Gly Glu Asp Asn Gly He Val Leu Glu Gly Ser Pro Leu Lys Gly Leu 115 120 • 125 Leu Wing Arg Ser Val He Val Val Asp Glu Asn Gly Lys Val Wing Tyr 130 135 140 Thr Gln Leu Val Asp Glu He Phe Thr Glu Pro Asp Tyr Asp Ala Wing 145 150 155 160 Leu Ala Gly Leu Asn 165 < 210 > 65 < 211 > 879 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (856) < 223 > RXN01676 < 400 > 65 agttacagct tttctcggtg gcacactcgc gctacttagc ccttgtgccg cactcctttt 60 accagcattt tttgcatcct cagtgggtgc tggcccgcgc atg atc ctt cac ggt 115 Met He Leu His Gly 1 5 gtt gtg ttc tac gca gga ctt cta gta ctt ctc gtg cca ctt ggc ctt 163 Val Val Phe Tyr Ala Gly Leu Leu Val Leu Leu Val Pro Leu Gly Leu 10 15 20 ggt gcg gga atc ctc ggc gag ctg ttt atc ccc ca g accc acc atc 211 Gly Wing Gly He Leu Gly Glu Leu Phe He Thr Gln Arg Gln Thr He 25 30 35 atc gtg gtt tea tcg atc gtg ctg att atc cta ggt ttt gtc cag atc 259 He Val Val Ser Ser He Val Leu He He Leu Gly Phe Val Gln He 40 45 50 ttc ggc ggc gga ttc gac ttc gga aaa gca ctc cca gga tta gat cgt 307 Phe Gly Gly Gly Phe Asp Phe Gly Lys Wing Leu Pro Gly Leu Asp Arg 55 60 65 ctg caa tct aag gcc act gtg acc g gt t g gt tta gga aag age ttt tta 355 Leu Gln Ser Lys Wing Thr Val Thr Ser Gly Leu Gly Lys Ser Phe Leu 70 75 80 85 cta gga atg acc agt agt att gcc gtt ttt tgt tcc gga cca atc ctc 403 Leu Gly Met Thr Be Ser Wing Wing Gly Phe Cys Ser Gly Pro He Leu 90 95 100 ggc gcc gtt ctt act ttg gct gcc acc agt gga aac tcc atc acc 451 Gly Wing Val Leu Thr Leu Ala Wing Thr Ser Gly Asn Ser He Thr Ser 105 110 115 gca ctc att ttg agt gct tat ggt gcg gga atg gtg ctg ccc ctg atg 499 Wing Leu He Leu Wing Wing Tyr Gly Wing Gly Met Val Leu Pro Leu Met 120 125 130 gct att gca gcg ctc tgg gcc aaa ctc gga cag cgt gga cag cag atg 547 Wing Wing Wing Wing Leu Trp Wing Lys Leu Gly Gln Arg Gly Gln Gln Met 135 140 145 ctc cgc ggc cgg gaa ttc acc ttc ttg ggc agg cag tgg cac att gtt 595 Leu Arg Gly Arg Glu Phe Thr Phe Leu Gly Arg Gln Trp His He Val 150 155 160 165 tct gtc att age ggt gcc ctg atc atc gct gtc gt atc ctc ttt tgg 643 Ser Val He Ser Gly Ala Leu He He Wing Val Gly He Leu Phe Trp 170 175 180 tcc acg aac ggc ctt gtc age atg ccg gag ctc gtt cca atg gac acc 691 Ser Thr Asn Gly Leu Val Ser Met Pro Glu Leu Val Pro Met Asp Thr 185 190 195 cag atc tgg cta cag gaa gcc here ttc tea ctc ggg tea cca ctc ttt 739 Gln He Trp Leu Gln Glu Ala Thr Phe Ser Leu Gly Ser Pro Le u Phe 200 205 210 gac atc gca ttg atc att gtc gcc gct ggc ttg ttc ttg tc tc tgg 787 Asp He Ala Leu He He Val Wing Wing Ala Gly Leu Phe Leu Tyr Phe Trp 215 220 225 aac aaa cga caa aag cga aaa gaa gaa gct cag cga ccc aaa gaa agt 835 Asn Lys Arg Gln Lys Arg Lys Glu Glu Wing Gln Arg Pro Lys Glu Ser 230 235 240 245 gga tgg gtt att aac ect cgc taattattag ttttggagcg agg 879 Gly Trp Val He Asn Pro Arg 250 < 210 > 66 < 211 > 252 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 66 Met He Leu His Gly Val Val Phe Tyr Ala Gly Leu Leu Val Leu Leu 1 5 10 15 Val Pro Leu Gly Leu Gly Wing Gly He Leu Gly Glu Leu Phe He Thr 20 25 30 Gln Arg Gln Thr He He Val Val Ser He Val Leu He He Leu 35 40 45 Gly Phe Val Gln He Phe Gly Gly Phe Asp Phe Gly Lys Ala Leu 50 55 60 'Pro Gly Leu Asp Arg Leu Gln Ser Lys Wing Thr Val Thr Ser Gly Leu 65 70 75 80 Gly Lys Ser Phe Leu Leu Gly Met Thr Ser Be He Wing Gly Phe Cys 85 90 95 Ser Gly Pro He Leu Gly Wing Val Leu Thr Leu Wing Ala Thr Ser Gly 100 105 110 Asn Ser He Thr Ser Ala Leu He Leu Ser Ala Tyr Gly Ala Gly Met 115 120 125 Val Leu Pro Leu Met Ala Ala Ala Ala Leu Trp Ala Lys Leu Gly Gln 130 135 140 Arg Gly Gln Gln Met Leu Arg Gly Arg Glu Phe Thr Phe Leu Gly Arg 145 150 155 • 160 Gln Trp His He Val Ser Val He Ser Gly Ala Leu He He Wing Val 165 170 175 Gly He Leu Phe Trp Ser Thr Asn Gly Leu Val Ser Met Pro Glu Leu 180 185 190 Val Pro Met Asp Thr Gln He Trp Leu Gln Glu Ala Thr Phe Ser Leu 195 200 205 Gly Ser Pro Leu Phe Asp He Wing Leu He He Val Wing Wing Gly Leu 210 215 220 Phe Leu Tyr Phe Trp Asn Lys Arg Gln Lys Arg Lys Glu Glu Wing Gln 225 230 235 240 Arg Pro Lys Glu Ser Gly Trp Val He Asn Pro Arg 245 250 < 210 > 67 < 211 > 744 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (721) < 223 > RXN00380 < 400 > 67 caggcaatgc gacctcgcct cagtgacatc cttggtgttc caagacgatc aaattgtcgg 60 cgtgcattac aacgaaccag ctcaggagat ttgatcactc gtg ttg cgt acc aaa 115 Leu Val Thr Lys Arg cta gca gca May 1 atc ggc tgc gtg here here ctc gga age gcg cta gta 163 .ctt Leu Wing Wing Thr He Gly Cys Val Thr Leu Ser Gl'y Leu Wing Leu Val 10 15 20 gcc tgc age agt gac agt acc gct ggt act gac gct gtt gct gtc ggc 211 Wing Cys Ser Ser Asp Ser Thr Wing Gly Thr Asp Wing Val Ala Val Gly 25 30 35 gga acc ttc caa ttc cac tcc ccg gat gga aag atg gaa att ttc tac 259 Gly Thr Phe Gln Phe His Ser Pro Asp Gly Lys Met Glu He Phe Tyr 40 45. 50 gac gag gct gac cgt caa caa ctc ccc gac att ggt gga gat tcc ctc 307 Asp Glu Wing Asp Arg Gln Gln Leu Pro Asp He Gly Gly Asp Ser Leu 55 60 65 atg gag gag ggc here cag atc aac ctg tct gat gatt gaa aac caa gtt 355 Met Glu Glu Gly Thr Gln He Asn Leu Ser Asp Phe Glu Asn Gln Val 70 75 80, 85 gtc atc ctc aat gcg tgg ggg cag tgg tgt gca ccg tgc cgc tcc gaa 403 Val He Leu Asn Ala Trp Gly Gln Trp Cys Wing Pro Cys Arg Ser Glu 90 95 100 tcc gat gat ctc cag att atc cat gag gaa ctc caa gct gcc gga aac 451 Ser Asp Asp Leu Gln He He His Glu Glu Leu Gln Ala Wing Gly Asn 105 110. 115 ggc gac acc ect ggt ggc gtg gtc atc aat gtg gtc gtc gtc 499 Gly Asp Thr Pro Gly Gly Thr Val Leu Gly He Asn Val Arg Asp Tyr 120 125 130 ccc ccc gac atc gcc caa gac ttt gtc acc gac aac ggc ctt gat tac 547 Ser Arg Asp He Wing Gln Asp Phe Val Thr Asp Asn Gly Leu Asp Tyr 135 140 145 cca age att tac gat cca cca ttt atg gca gca tcc ctc ggt ggt 595 Pro Ser He Tyr Asp Pro Pro Phe Met Thr Ala Ala Ser Leu Gly Gly 150 155 160 165 gtt ccc gca tcg gtg atc cca acc acc atc gtg ctg gat aaa cag cac 643 Val Pro Ala Ser Val He Pro Thr He Val Leu Asp Lys Gln His 170 175 180 cgc ccc gca gca gtg ttc ttg cgc gaa gtc acc tcc aaa gat gtg ttg 691 Arg Pro Ala Ala Val Phe Leu Arg Glu Val Thr Ser Lys Asp Val Leu 185 190 195 gat gtt gcg ttg cca ttg gta gat gag gcc taaatgtctg agattgtggt 741 Asp Val Ala Leu Pro Leu Val Asp Glu Wing 200 205 age 744 < 210 > 68 < 211 > 207 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 68 Val Arg Leu Thr Lys Leu Wing Wing Thr He Gly Cys Val Thr Leu Ser 1 5 10 15 Gly Leu Wing Leu Val Wing Cys Ser Being Asp Ser Thr Wing Gly Thr Asp 20 25 30 Wing Val Wing Val Gly Gly Thr Phe Gln Phe His Ser Pro Asp Gly Lys 35 40 45 Met Glu He Phe Tyr Asp Glu Wing Asp Arg Gin Gln Leu Pro Asp He 50 55 60 Gly Gly Asp Ser Leu Met Glu Glu Gly Thr Gln He Asn Leu Ser Asp 65 70 75 80 Phe Glu Asn Gln Val Val He Leu Asn Wing Trp Gly Gln Trp Cys Wing 85 90 95 Pro Cys Arg Ser Glu Ser Asp Asp Leu Gln He He His Glu Glu Leu 100 105 110 Gln Wing Wing Gly Asn Gly Asp Thr Pro Gly Gly Thr Val Leu Gly He 115 120 125 Asn Val Arg Asp Tyr Ser Arg Asp He Wing Gln .Asp Phe Val Thr Asp 130 135 140 Asn Gly Leu Asp Tyr Pro Ser He Tyr Asp Pro Pro Phe Met Thr Ala 145 150 155 160 Wing Being Leu Gly Gly Val Pro Wing Being Val He Pro Thr Thr He Val 165 170 175 Leu Asp Lys Gln His Arg Pro Ala Wing Val Phe Leu Arg Glu Val Thr 180 185 190 Ser Lys Asp Val Leu Asp Val Wing Leu Pro Leu Val Asp Glu Wing 195 200 205 < 210 > 69 < 211 > 495 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (472) < 223 > RXN00937 < 400 > 69 agctgccggt caatgaagaa aatccttggc cgggaataac tacagtccgc tgaaagttgg 60 tctatatata gaccttacaa atcttgaacg gagattctta atg gca acc atc gat 115 Met wing Thr He Asp 1 '5 gta acc gaa gaa here ttt gag age here gtt acc ggc gac gga att gtc 163 Val Thr Glu Glu Thr Phe Glu Be Thr Val Thr Gly Asp Gly He Val 10 15 20 ctc gta gac gca tgg gca tcc tgg tgc gga ect tgc cgc cag tc gcc 211 Leu Val Asp Wing Trp Wing Ser Trp Cys Gly Pro Cys Arg Gln Phe Wing 25 30 35 cca acc tac gag aag gtt tcc gaa acc cac acc gac gca acc ttc gcc 259 Pro Thr Tyr Glu Lys Val Ser Glu Thr His Thr Asp Ala Thr Phe Wing 40 45 50 aag ctt gat acc gaa gca aac cag ggc ctg gct gca gca ctg cag atc 307 Lys Leu Asp Thr Glu Wing Asn Gln Gly Leu Wing Wing Ala Leu Gln He 55 60 65 cag tcc atc cca act ctg atg gtt ttc cgc gac ggc atc atg gtc tac 355 Gln Ser He Pro Thr Leu Met Val Phe Arg Asp Gly He Met Val Tyr 70 75 80 85 cgc gaa gcc ggc acc atg cca gct ect gca ctg gat gat ctg gtc aac 403 Arg Glu Wing Gly Thr Met Pro Wing Pro Wing Leu Asp Asp Leu Val Asn 90 95 100 cag gtt aag gca ctc gac atg gat gac gtt cgt cgc cag gtc gca gag 451 Gln Val Lys Ala Leu Asp Met Asp Asp Val Arg Arg Gln Val Ala Glu 105 110 115 cag cag ggt tct gca gag gca taagetteca attgtgtttt ggt 495 Gln Gln Gly Ser Ala Glu Ala 120 < 210 > 70 < 211 > 124 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 70 Met Wing Thr He Asp Val Thr Glu Glu Thr Phe Glu Ser Thr Val Thr 1 5 10 15 Gly Asp Gly He Val Leu Val Asp Wing Trp Wing Ser Trp Cys Gly Pro 20 25 30 Cys Arg Gln Phe Wing Pro Thr Tyr Glu Lys Val Ser Glu Thr His Thr 35 40 45 Asp Wing Thr Phe Wing Lys Leu Asp Thr Glu Wing Asn Gln Gly Leu Wing 50 55 60 Wing Wing Gln He Gln He He Pro Thr Leu Met Val Phe Arg Asp 65 70 75 '• 80 Gly He Met Val Tyr Arg Glu Wing Gly Thr Met Pro Pro Wing Ala Leu 85 90 _ 95 Asp Asp Leu Val Asn Gln Val Lys Ala Leu Asp Met Asp Asp Val Arg 100 105 • 110 Arg Gln Val Wing Gllu Gln Gln Gly Ser Wing Glu Ala 115 120 < 210 > 71 a * jV t 'j -' * "» • "- < 211 > 990 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (967) < 223 > RXN02325 < 400 > 71 cagagatttg aagatggaga ccaaggctca aagggaatcc atgccgtctt ggtttaatac 60 tgcacccgtc taatgaaaat cattactatt aggtgtcatg atg gac cat gca cac 115 Met Asp His Ala His 1 5 gat tcc tgc tea cca act ctc cgc cgt gat ttg gag gtc act ggc cag 163 Asp Ser Cys Ser Pro Thr Leu Arg Arg Asp Leu Glu Val Thr Gly Gln 10 15 20 ctc caa ect gag aaa gct gtc gatta gca gcg ccg cac gaa ggg aag 211 Leu Gln Pro Glu Lys Ala Val Asp Leu Ala Ala Pro His Glu Gly Lys 25 30 35 gtt gcc aat ata acg aag gtg acc tcc tea aat atg gag cac acc atc 259 Val Ala Asn He Thr Lys Val Thr Ser Ser Asn Met Glu His Thr He 40 45 50 acg cag gcc tea aaa gct aag gag gtg gtg gtg ctc att ggt cac tcc 307 Thr Gln Ala Ser Lys Wing Lys Glu Val Val Val Leu He Gly His Ser 55 60 65 ctg ctg ccc here ttt cag gat ttg gaa aaa gac att ctg cac ttt cag 355 Leu Leu Pro Thr Phe Gln Asp Leu Glu Lys Asp 'He Leu His Phe Gln 70 75 80 85 gca ggt aat aaa ggg cga ttt tct gta gcg att gtt gat ect gat cgc 403 Wing Gly Asn Lys Gly Arg Phe Ser Val Wing He Val Asp Pro Asp Arg 90 95 100 agt gca gat gtg gtt gcc aga ttt agg cca aaa cag att ccg gtg gca 451 Be Ala Asp Val Val Ala Arg Phe Arg Pro Lys Gln He Pro Val Ala 105 110 115 tac gtg gtg aaa gat ggc gcc age att gcg gag ttc aac tcg ctc aac 499 Tyr Val Val Lys Asp Gly Ala Ser He Wing Glu Phe Asn Ser Leu Asn 120 125 • 130 aag gag ccg gtt gca caa tgg ctt gat cat ttt gtg tcg cgg gaa acg 547 Lys Glu Pro Val Wing Gln Trp Leu Asp His Phe Val Ser Arg Glu Thr 135 140 145 atc ccc aat gaa aaa gag ggg gac gtc gat aag caa ata gac ccg cgc 595 He Pro Asn Glu Lys Glu Gly Asp Val Asp Lys Gln > He Asp Pro Arg 150 155 160 • 165 ctg tgg cgg gca gcg gaa ttg gtg aac gcc ggt gat ttt cgc gcg gcg 643 Leu Trp Arg Ala Ala Glu Leu Val Asn Ala Gly A'sp Phe Arg Ala Ala 170 175 180 ttg gcg ttg tat gag cag ttg ccg cag gat gcg acg gtg aag cgg gcg 691 Leu Ala Leu Tyr Glu Gln Leu Pro Gln Asp Wing Thr Val Lys Arg Wing 185 190 195 cac gcg gcg gtg tcg gta ttg gcg cgg atg tct gtg gcg gat cgg gga 739 His Ala Ala Val Ser Val Leu Ala Arg Met Ser Val 'Ala Asp Arg Gly 200 205. 210 gag gat ccg atc gag aag tcg cgc cgg gat cca gac. gat gtg aac aag 787 Glu Asp Pro He Glu Lys Ser Arg Arg Asp Pro Asp Asp Val Asn Lys 215 220 225 gcg ctg gcg gcg gcg gat atg tat gtg ttg atg aat cag ccg gac here 835 Wing Leu Wing Wing Asp Met Tyr Val Leu Met Asn Gln Pro Asp Thr 230 235 240 245 gcg ctc gcg cac ctt gca gca cta ttg cca aaa ccg gag gct gcc cgg 883 Ala Leu Ala His Leu Ala Ala Leu Pro Lys Pro Glu Ala Ala Arg 250 255 260 cgg atc gtg gag ttg ctg aac ttg ttt gat ccg ctg gac ctg gtc gca 931 Arg He Val Glu Leu Leu Asn Leu Phe Asp Pro Leu Asp Leu Val Wing 265 270 275 ttg gaa atc agg gcg cag gtg ggg aat gca atg age taagaaaaca 977 Leu Glu He Arg Wing Gln Val Gly Asn Wing Met Ser 280 285 ctttaaatat tct 990 < 210 > 72 < 211 > 289 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 72 Met Asp His Wing His Asp Ser Cys Ser Pro Thr Leu Arg Arg Asp Leu 1 5 10 _ 15 Glu Val Thr Gly Gln Leu Gln Pro Glu Lys Wing Val Asp Leu Wing Wing 20 25 30 Pro His Glu Gly Lys Val Wing Asn He Thr Lys Val Thr Ser Ser Asn 35 40 45 Met Glu His Thr He Thr Gln Ala Ser Lys Ala Lys Glu Val Val Val 50 55 60 Leu He Gly His Ser Leu Leu Pro Thr Phe Gln Asp Leu Glu Lys Asp 65 70 75 80 He Leu His Phe Gln Wing Gly Asn Lys Gly Arg Phe Ser Val Wing He. 85 90 95 Val Asp Pro Asp Arg Ser Wing Asp Val Val Wing Arg Phe Arg Pro Lys 100 105 '110 Gln He Pro Val Wing Tyr Val Val Lys Asp Gly Wing Ser He Wing Glu 115 120 125 Phe Asn Ser Leu Asn Lys Glu Pro Val Ala Gln Trp Leu Asp His Phe 130 135 140 • Val Ser Arg Glu Thr He Pro Asn Glu Lys Glu Gly Asp Val Asp Lys 145 150 155 160 Gln He Asp Pro Arg Leu Trp Arg Ala Ala Glu Leu Val Asn Ala Gly 165 170 175 Asp Phe Arg Ala Ala Ala Leu Ala Leu Tyr Glu Gln Leu Pro Gln Asp Wing 180 185 190 Thr Val Lys Arg Ala His Wing Wing Val Ser Val Leu Wing Arg Met Ser 10 195 200 205 Val Wing Asp Arg Gly Glu Asp Pro He Glu Lys Ser Arg Arg Asp Pro 210 215 220 Asp Asp Val Asn Lys Ala Leu Ala Wing Wing Asp Met Tyr Val Leu Met 225 230 235 240 Asn Gln Pro Asp Thr Ala Leu Ala His Leu Ala Ala Leu Leu Pro Lys 245 250 255 Pro Glu Ala Ala Arg Arg He Val Glu Leu Leu Asn Leu Phe Asp Pro 15 260 265 270 Leu Asp Leu Val Ala Leu Glu He Arg Ala Gln Val Gly Asn Ala Met 275 280 285 Ser < 210 > 73 < 211 > 900 < 212 > DNA 20 < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (877) < 223 > RXN01837 < 400 > 73 cccccatcat tccctcaagg tgtgaagata cggttaggat agaaaagaat ttttttgacg 60 ttggacattc tcaaaatcaa gtagcaaggg atcaaactct gtg agt act aat aag 115 Val Ser Thr Asn Lys 25 1 5 gaa cga cgc caa cag gcg ctt ctg cag ctg gag aaa gaa atc aaa age 163 Glu Arg Arg Gln Gln Ala Leu Be Gln Leu Glu Lys lu He Lys Ser 10 15 20 cgg gac cgc aaa gaa aag acc aag cca cta acc gtg gtc ttt gct tcc 211 Arg Asp Arg Lys Glu Lys Thr Lys Pro Leu Thr Val Val Phe Wing Ser 25 30 35 ctg gct gtc atc ctg gtt gtc gtt ggc ggt atc tgg tac gca gct acc 259 Leu Ala Val He Leu Val Val Val Gly Gly He Trp Tyr Ala Ala Thr 40 45 '50 cgc age acc gaa gac gac gtc atc ac gct gat gaa here tcc acc Acc 307 Arg Ser Thr Glu Asp Glu Val He Thr Wing Asp Glu Thr Ser Thr Thr 55 60 65 gca gag acc ect gac tac cag cca ctg gcg ctg acc cgc acc acc gcg 355 Wing Glu Thr Pro Asp Tyr Gln Pro Leu Ala Leu Thr Arg Thr Thr Wing 70 75 80 '85 ctc ggc gac tcc gtg acc tgt gag tac cca gat gct ggc gact gct tcc 403 Leu Gly Asp Ser Val Thr Cys Glu Tyr Pro Asp Wing G and Glu Ala Ser 90 95 100 aag gat gtc tcc aag ect gct act gaa aac gtg cca gca acc ggc acc 451 Lys Asp Val Ser Lys Pro Wing Thr Glu Asn Val Pro Wing Thr Gly Thr 105 110 115 gtg acc gtc aac ctg acc acc gcc cag ggc aac atc ggc atg gaa ctt 499 Val Thr Val Asn Leu Thr Thr Wing Gln Gly Asn lié Gly Met Glu Leu 120 125 130 gat cgc tcc gcc tcc ect tgt acc gtc aac gct 'gtt gag cac atg gct 547 Asp Arg Ser Val Ser Pro Cys Thr Val Asn Wing Val Glu His Met Wing 135 140 145 tcc gag ggc tac tac aac gat act gtc tcc cac cgc atc acc action 595 Ser Glu Gly Tyr Tyr Asn Asp Thr Val Cys His Arg He Thr Thr Ser 150 155 160 165 ggc att tac gtt ctc cag tgc ggc gat cca age age acc ggc gca ggc 643 Gly He Tyr Val Leu Gln Cys Gly Asp Pro Ser Ser Thr Gly Wing Gly 170 175 180 ggc cca ggg ttc age ttc gcc aac gaa tac cca acc gac gaa gca act 691 Gly Pro Gly Phe Ser Phe Wing Asn Glu Tyr Pro Thr Asp Glu Wing Thr 185 190 195 gac cta acc acc ac cc gc atc tac gac cgc gcc acc atc gcc atg gcc 739 Asp Leu Thr Thr Pro Val He Tyr Glu Arg Gly Thr He Wing Met Wing 200 205 210 aac gct ggc gct gac acc aac ggg ctc cca gtt ctt ect caa cta cga 787 Asn Wing Gly Wing Asp Thr Asn Gly Leu Pro Val Leu Pro Gln Leu Arg 215 220 225 gga ttc ccc act ggc acc gaa cta cac cta ctt cgg cca gat cac cga 835 Gly Phe Pro Thr Gly Thr Glu Leu His Leu Leu Arg Pro Asp His Arg 230 235 240 245 aga agg ect tgc aac ect cga cgc cat cgc aga agt tgg cac 877 Arg Arg Pro Cys Asn Pro Arg Arg His Arg Arg Ser Trp His 250 255 tgaaggtgga accggcgacg gag 900 < 210 > 74 < 211 > 259 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 74 Val Ser Thr Asn Lys Glu Arg Arg Gln Gln Ala Leu Ser Gln Leu Glu 1 5 10 15 Lys Glu He Lys Ser Arg Asp Arg Lys Glu Lys Thr Lys Pro Leu Thr 20 25 '30 Val Val Phe Ala Ser Leu Ala Val He Leu Val Vai Val Gly Gly He 35 40 45 Trp Tyr Wing Wing Thr Arg Ser Thr Glu Asp Glu Val He Thr Wing Asp 50 55 60 Glu Thr Ser Thr Thr Wing Glu Thr Pro Asp Tyr Gln Pro Leu Wing Leu 65 70 75 80 Thr Arg Thr Thr Ala Leu Gly Asp Ser Val Thr Cys Glu Tyr Pro Asp 85 90 95 Wing Gly Glu Wing Ser Lys Asp Val Ser Lys Pro Wing Thr Glu Asn Val 100 105 110 Pro Wing Thr Gly Thr Val Thr Val Asn Leu Thr Thr Wing Gln Gly Asn 115 120 '125 He Gly Met Glu Leu Asp Arg Ser Val Ser Pro Cys Thr Val Asn Wing 130 135 140 Val Glu His Met Wing Ser Glu Gly Tyr Tyr Asn Asp Thr Val Cys His 145 150 155 160 Arg He Thr Thr Ser Gly He Tyr Val Leu Gln Cys Gly Asp Pro Ser 165 170 175 Ser Thr Gly Wing Gly Gly Pro Gly Phe Ser Phe Wing Asn Glu Tyr Pro 180 185 190 Thr Asp Glu Wing Thr Asp Leu Thr Thr Pro Val He Tyr Glu Arg Gly 195 200 '205 Thr He Wing Met Wing Wing Asn Wing Gly Wing Asp Thr Asn Gly Leu Pro Val 210 215 220 Leu Pro Gln Leu Arg Gly Phe Pro Thr Gly Thr Glu Leu His Leu Leu 225 230 235 240 Arg Pro Asp His Arg Arg Arg Pro Cys Asn Pro Arg Arg His Arg Arg 245 250 255 Ser Trp His < 210 > 75 < 211 > 741 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (741) < 223 > RXN01926 < 400 > 75 ctg cga age ttc tac acc cca gaa caa gcc atc gaa cgc gaa ggc gac 48 Leu Arg Ser Phe Tyr Thr Pro Glu Gln Wing He Glu Arg Glu Gly Asp 1 5 10 15 gtc tgg aaa gcc gcc acc gaa gaa gca gaa ctc ctc gca gct gac ggc 96 Val Trp Lys Ala Ala Thr Glu Glu Ala Glu Leu Leu Ala Ala Asp Gly 20 25 30 gcc gtc cac gac cag gaa ctc ttc ctc aac tgc acc acc tcc cca ctg 144 Wing Val His Asp Gln Glu Leu Phe Leu Asn Cys Thr Thr Ser Pro Leu 35 40 45 atc tcc gcc tc gcg atg ctc aac ttc ggc gcc cac caa atc ctg gac 192 He Phe Wing Being Wing Met Leu Asn Phe Gly Val His Gln He Leu Asp 50 55 60 acc ctc tcc tc ccac cca cca cca ccc ccc gcc cgc gac gca gac ccc 240 Thr Leu Cys Gln Leu Wing Pro Ser Pro Wing Gly Arg Asp Wing Asp Pro 65 70 75 80 aaa gcc ctc gaa gcc gcc gcc action acc gca atg gac gac cac cgc gac acc 288 Lys Wing Leu Glu Wing Wing Thr Ser Wing Met Asp Asp His Arg Asp Thr 85 90 95 acc gac gac ttc tcc ggc gtc gtc ttc aaa gtc caa .. gcc ggc atg gac 336 Thr Asp Asp Phe Ser Gly Val Val Phe Lys Val Gln Wing Gly Met Asp 100 105 110 aaa aac cac cgc gat acc ctc gcc ttc atg cgc gtc gtc tcc ggc gaa 384 Lys Asn His Arg Asp Thr Leu Wing Phe Met Arg Val Val Ser Gly Glu 1 15 120 • 125 tcc gac cgc ggc atg caa gtc acccccc ccc tcc ggc cgc age ttc 432 Phe Asp Arg Gly Met Gln Val Thr His Ser Gln Ser Gly Arg Ser Phe 130 135 140 tcc acc aaa tac gcc ctc acc gtc ttc ggc cgc acc cgc tct acc gtc 480 Ser Thr Lys Tyr Ala Leu Thr Val Phe Gly Arg Thr Arg Ser Thr Val 145 150 155 160 gaa acc gcc ttc ccc ggc gac atc gtc ggc ctc gtc aac gcc ggc gcc 528 Glu Thr Ala Phe Pro Gly Asp He Val Gly Leu Val Asn Wing Gly Wing 165 170 175 • ctc gca cca ggc gac acc atc ttc gaa ggc cga aaa atc caa tac cca 576 Leu Wing Pro Gly Asp Thr He Phe Glu Gly Arg Lys He Gln Tyr Pro 180 185 190 cca atg cca aaa ttc gcg cca gaa cac tcc ccc atc ctc cgc gcc aaa 624 Pro Met Pro Lys Phe Pro Pro Glu His Phe Arg He Leu Arg Ala Lys 195 200 205 tea ctc ggc aaa tac aaa cag ttc cgc aaa gcc ctc gag cag ctg gac 672 Ser Leu Gly Lys Tyr Lys Gln Phe Arg Lys Wing Leu Glu Gln Leu Asp 10 210 215 220 tcc gaa ggt gtc gtc cag atc ctc aag aac gac ctg cgt ggc gac gcc 720 Ser Glu Gly Val Val Gln He Leu Ly s Asn Asp Leu Arg Gly Asp Ala • 225 230 235 240 aac cca ggt cat ggc cgg tgt 741 Asn Pro Gly His Gly Arg Cys 245 < 210 > 76 15 < 211 > 247 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 76 Leu Arg Ser Phe Tyr Thr Pro Glu Gln Wing He Glu Arg Glu Gly Asp 1 5 10 '15 Val Trp Lys Wing Wing Thr Glu Glu Wing Glu Leu Leu Wing Wing Asp Gly 20 25 30 Wing Val His Asp Gln Glu Leu Phe Leu Asn Cys Thr Thr Ser Pro Leu 20 35 40 45 He Phe Wing Being Wing Met Leu Asn Phe Gly Val His Gln He Leu Asp 50 55 60 Thr Leu Cys Gln Leu Wing Pro Pro Pro Wing Gly Arg Asp Wing Asp Pro 65 70 75 80 Lys Wing Leu Glu Wing Wing Thr Ser Wing Met Asp Asp His Arg Asp Thr 85 90 95 Thr Asp Asp Phe Ser Gly Val Val Phe Lys Val Gln Wing Gly Met Asp 25 100 105 110 Lys Asn His Arg Asp Thr Leu Wing Phe Met Arg Val Val Ser Gly Glu 115 120. 125 Phe Asp Arg Gly Met Gln Val Thr His Ser Gln Ser Gly Arg Ser Phe 130 135 140 Ser Thr Lys Tyr Ala Leu Thr Val Phe Gly Arg Thr Arg Ser Thr Val 145 150 155 160 Glu Thr Wing Phe Pro Gly Asp He Val Gly Leu Val Asn Wing Gly Wing 165 170 175 Leu Wing Pro Gly Asp Thr He Phe Glu Gly Arg Lys He Gln Tyr Pro 180 185 190 Pro Met Pro Lys Phe Wing Pro Glu His Phe Arg He Leu Arg Ala Lys 195 200 205 Ser Leu Gly Lys Tyr Lys Gln Phe Arg Lys Ala Leu Glu Gln Leu Asp 210 215 220 Ser Glu Gly Val Val Gln He Leu Lys Asn Asp Leu Arg Gly Asp Ala 225 230 235 240 Asn Pro Gly His Gly Arg Cys 245 < 210 > 77 < 211 > 478 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (478) < 223 > RXN02002 < 400 > 77 aagtggcaaa aaacgtttca agcaggcaac gccggcgtac aacttcgctg agctggggcg agtggggcc agcgcccaca acccgctatt cttaataccc atg age aac gcc aat 115 Met Ser Asn Wing Asn 1 5 tcc gac acc acc gcc gacc gca cat cgc cgc aga here tcc gcc gta 163 Ser Asp Thr Thr Ala Ala Glu Ala His Arg Arg Arg Thr Phe Wing Val 10 15 20 atc gca cac ccc gac gcc ggt aaa tcc acc ctc acc gag gca ttg gcg 211 He Ala His Pro Asp Ala Gly Lys Ser Thr Leu Thr Glu Ala Leu Ala 25 30 «35 ctg cat gca cac atc atc tcc gaa gcc ggc gcc acc cac ggc aaa gca 259 Leu His Wing His He He Ser Glu Wing Gly Wing Thr His Gly Lys Wing 40 45 50 ggc cgc aaa gcc acc gtt tcc gag tgg gag tg atg gaa aaa gac cgc 307 Gly Arg Lys Wing Thr Val Ser Asp Trp Met Glu Met Glu Lys Asp Arg 55 60 65 ggc atc tcc atc gcc tcc tcc gca ctc caa ttc gag tac gca cca gaa 355 Gly He Ser Be Ala Be Ser Ala Leu Gln Phe Glu Tyr Wing Pro Glu 70 75 80 85 ggc cac gca ggc gag ccc ttc atc atc aac ctc gtg gac acc cca ggc 403 Gly His Wing Gly Glu Pro Phe Met He Asn Leu Val Asp Thr Pro Gly 90 95 100 cac gcc gac ttc tcc gaa gac acc tac cgc gtc ctc atg gcc gtc gac 451 His Wing Asp Phe Ser Glu Asp Thr Tyr Arg Val Leu Met Wing Val Asp 105 110 115 gca gca gtc atg ctt atg cac tcc gtc 478 Ala Ala Val Met Leu Met His Ser Val 120 125 < 210 > 78 < 211 > 126 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 78 Met Ser Asn Wing Asn Being Asp Thr Thr Wing Wing Glu Wing His Arg Arg 1 5 10 15 Arg Thr Phe Wing Val He Wing His Pro Asp Wing Gly Lys Ser Thr Leu 20 25 30 Thr Glu Wing Leu Wing Leu His Wing His He He Ser Glu Ala Gly Ala 35 40 45 Thr His Gly Lys Wing Gly Arg Lys Wing Thr Val Ser Asp Trp Met Glu 50 55 60 Met Glu Lys Asp Arg Gly He Ser Be Ala Be Ser Ala Leu Gln Phe 65 70 75 80 Glu Tyr Ala Pro Glu Gly His Wing Gly Glu Pro Phe Met He Asn Leu 85 90 95 Val Asp Thr Pro Gly His Wing Asp Phe Ser Glu Asp Thr Tyr Arg Val 100 105 110 Leu Met Ala Ala Asp Ala Ala Val Met Leu Met His Ser Val 115 120. 125 < 210 > 79 < 211 > 1080 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1057) < 223 > RXN02736 < 400 > 79 cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca 60 • acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115 Met He Phe Glu Leu 1 5 ccg gat acc acc acga cag att tcc aag ac cta act cga ctg cgt 163 Pro Asp Thr Thr Thr Gln Gln He Ser Lys Thr Leu Thr Arg Leu Arg 10 15 20 gaa tcg ggc acc ggc acc acc ggc acc ggc gtc ctc acc ctc atc gtg 211 Glu Be Gly Thr Gln Val Thr Thr Gly Arg Val Leu Thr Leu He Val 25 30 _ 35 10 gtc act gac tcc gaa age gat gtc gct gca gtt acc gag tcc action aat 259 Val Thr Asp Ser Glu Be Asp Val Wing Wing Val Thr Glu Ser Thr Asn 40 45 50 gaa gcc tcg cgc gag cac cca tct cgc gtg atc att ttg gtg gtt ggc 307 Glu Ala Ser Arg Glu His Pro Ser Arg Val He He Leu Val Val Gly 55 60 65 gat aaa act gca gaa aac aaa gtt gac gca gaa gtc cgt atc ggt ggc 355 Asp Lys Thr Wing Glu Asn Lys Val Asp Wing Glu Val Arg He Gly Gly 70 75 80 85 15 gac gct ggt gct tc gag atg atc atc atc cat ctc aac gga ect gtc 403 Asp Wing Gly Wing Glu Met Met He He Met His Leu Asn Gly Pro Val 95 95 '100 gct gac aag ctc cag tat gtc gtc ac cca ctg ttg ctt gac acc 451 Ala ect Lys Leu Gln Asp Val Val Tyr Thr Pro Leu Leu Leu Pro Asp Thr 105 110 '115 ccc atc gtt gct tgg tgg ggt cca aat gaa aag cca tea cag 499 Pro ect aka I Val Wing Trp Trp Pro Gly Glu Pro Pro Lys Asn Pro Ser Gln 120 125 130 20 gac cca att gga cgc atc gca caca cga cgc atc act gat gct ttg tac 547 Asp Pro He Gly Arg He Wing Gln Arg Arg He Thr Asp Ala Leu Tyr 135 140 145 gac cgt gat gac gca cta gaa gat cgt gtt gag aac tat cac cca ggt 595 Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu Asn Tyr His Pro Gly 150 155 160 165 gat acc gac atg acg tgg gcg cgc ctt acc cag tgg cgg gga ctt gtt 643 Asp Thr Asp Met Thr Trp Wing Arg Leu Thr Gln Trp Arg Gly Leu Val 170 175 '180 25 gcc tcc tea ttg gat cac cca cca cac age gaa atc act tcc gtg agg 691 Wing Being Ser Leu Asp His Pro Pro His Ser Glu He Thr Ser Val Arg 185 190 195 ctg acc ggt gca age ggc agt acc tcg gtg gat ttg gct gca ggc tgg 739 Leu Thr Gly Wing Ser Gly Ser Thr Ser Val Asp Leu Wing Ala Gly Trp 200 205 210 5 ttg gcg cgg agg ctg aaa gtg ect gtg atc cgc gag gtg here gat gct 787 • Leu Wing Arg Arg Leu Lys Val Val Val Ar Ar Glu Val Thr Asp Ala 215 220 225 ccc acc gtg cca acc gat gag ttt ggt act cca ctg ctg gct atc cag 835 Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro Leu Leu Ala He Gln 230 235 240 245 cgc ctg gag atc gtt cgc acc acc ggc tcg atc atc atc tat atc tat 883 Arg Leu Glu He Val Arg Thr Thr Gly Ser He lie He Thr He Tyr 250 255 260 10 gac gct cat acc ctt cag gta gag atg ccg gaa tcc ggc aat gcc cca 931 Asp Ala His Thr Leu Gln Val Glu Met Pro Glu Ser Gly Asn Ala Pro 265 270 275 • tcg ctg gtg gct att ggt cgt cga agt gag tcc gac tgc ttg tct gag 979 Ser Leu Val Ala He Gly Arg Arg Ser Glu Ser Asp Cys Leu Ser Glu 280 285 290 gag ctt cgc cac atg gat cca gat ttg ggc tac cag cac gca cta tcc 1027 Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr Gln His Ala Leu Ser 295 300 305 15 ggc ttg tcc age gtc aag ctg gaa acc gtc taaggagaaa tacaacacta 1077 Gly Leu Ser Ser Val Lys Leu Glu Thr Val 310 315 tgg 1080 < 210 > 80 < 211 > 319 < 212 > PRT < 213 > Corynebacterium glutamicum 20 < 400 > 80 Met He Phe Glu Leu Pro Asp Thr Thr Thr Gln Gln He Ser Lys Thr 1 5 10 15 Leu Thr Arg Leu Arg Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val 20 25 '. 30 Leu Thr Leu He Val Val Thr Asp Ser Glu Ser Asp Val Ala Wing Val 35 40 45 Thr Glu Ser Thr Asn Glu Wing Ser Arg Glu His Pro Ser Arg Val He 50 55 60 25 He Leu Val Val Gly Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu 65 70 75 80 Val Arg He Gly Gly Asp Wing Gly Wing Ser Glu Met He He Met His 85 90 95 Leu Asn Gly Pro Val Wing Asp Lys Leu Gln Tyr Val Val Thr Pro Leu 100 105 * '110 Leu Leu Pro Asp Thr Pro He Val Wing Trp Trp Pro Gly Glu Ser Pro 115 120 125 Lys Asn Pro Ser Gln Asp Pro He Gly Arg He Wing Gln Arg Arg He 130 135 140 Thr Asp Wing Leu Tyr Asp Arg Asp Asp Wing Leu Glu Asp Arg Val Glu 145 150 155 160 Asn Tyr His Pro Gly Asp Thr Asp Met Thr Trp Wing Arg Leu Thr Gln 165 170 175 Trp Arg Gly Leu Val Wing Being Ser Leu Asp His Pro Pro His Ser Glu 180 185 190 He Thr Ser Val Arg Leu Thr Gly Wing Ser Gly Ser Thr Ser Val Asp 195 200 205 Leu Wing Wing Gly Trp Leu Wing Arg Arg Leu Lys Val Pro Val He Arg 210 215 220 Glu Val Thr Asp Wing Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro 225 230 235 _ 240 Leu Leu Ala He Gln Arg Leu Glu He Val Arg Thr Thr Gly Ser He 245 250 255 He He Thr He Tyr Asp Ala His Thr Leu Gln Val Glu Met Pro Glu 260 265 270 Ser Gly Asn Ala Pro Ser Leu Val Ala He Gly Arg Arg Ser Glu Ser 275 280 '285 Asp Cys Leu Ser Glu Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr 290 295 300 Gln His Wing Leu Ser Gly Leu Ser Ser Val Lys Leu Glu Thr Val 305 310 315 < 210 > 81 < 211 > 331 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (301) < 223 > RXS03217 < 400 > 81 tctgtgaagg tagatggttt gacgaggagt tccaacgact cggacgctgg tgaatcatgc 60 tggcgaacgt agcatcacct gattaggaaa aggtacaaat atg gca cag ggt act 115 Met Wing Gln Gly Thr 1 5 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac 163 Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly Phé He Wing Pro Asn 10 15 20 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc 211 Asp Gly Ser Wing Asp Leu Phe Val His Tyr Ser Glu He Gln Gly Ser 25 30. ' 35 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc 259 Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val Glu Phe Glu Val Gly 40 45 50-gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc 301 Glu Gly Wing Lys Gly Pro Gln Wing Gln Gln Val Arg Wing Leu 55 60 65 taagctctaa ctgctagcta aaaattccgc 331 < 210 > 82 < 211 > 67 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 82 Met Wing Gln Gly Thr Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly 1 5 10 15 Phe He Wing Pro Asn Asp Gly Ser Wing Asp Leu Phe Val His Tyr Ser 20 25 30 Glu He Gln Gly Ser Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val 35 40 45 Glu Phe Glu Val Gly Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val 50 55 .60 Arg Ala Leu 65 < 210 > 83 < 211 > 324 < 212 > DNA <; 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (301) < 223 > FRXA01917 < 400 > 83 tctgtgaagg tagatggttt gacgaggagt tccaacgact cggacgctgg tgaatcatgc 60 tggcgaacgt agcatcacct gattaggaaa aggtacaaat atg gca cag ggt act 115 Met Wing Gln Gly Thr 1 5 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac 163 Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly Phe He Wing Pro Asn 10 15 '20 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc 211 Asp Gly Ser Wing Asp Leu Phe Val His Tyr Ser Glu He Gln Gly Ser 25 30 35 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc 259 Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val Glu Phe Glu Val Gly 40 45 50 gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc 301 Glu Gly Wing Lys Gly Pro Gln Wing Gln Gln Val Arg Wing Leu 55 60 65 taagctctaa ctgctagcta aaa 324 < 210 > 84 < 211 > 67 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 84 Met Wing Gln Gly Thr Val Lys Trp Phe Asn Gly Glu Lys Gly Phe Gly 1 5 10 '15 Phe He Wing Pro Asn Asp Gly Ser Wing Asp Leu Phe Val His Tyr Ser 20 25 30 Glu He Gln Gly Ser Gly Phe Arg Asn Leu Glu Glu Asn Gln Pro Val 35 40 45 Glu Phe Glu Val Gly Glu Gly Ala Lys Gly Pro Gln Ala Gln Gln Val 50 55 60 Arg Ala Leu 65 < 210 > 85 < 211 > 504 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (481) < 223 > RXA02184 < 400 > 85 tttaccgcga tgcttgcggt gtataataat ttcttctggt caaaaatagt tgatcaattt 60 gaatcagcat atgaattagg aatgaaagtg gtgaggacaa gtg ect gtc gga gga here 115 Val Pro Val Gly Thr 1 5 gtg aag tgg tac gac gcg gag cgt ggt ttc ggc ttt gtc tcc aat cca 163 Val Lys Trp Tyr Asp Ala Glu Arg Gly Phe Gly Phe Val Ser Asn Pro 10 15 20 ggt ggt gaa gat tgc ttc gta ggt aag caa gta ctt ccc aag gga gtc 211 Gly Gly Glu Asp Cys Phe Val Gly Lys Gln Val Leu Pro Lys Gly Val 25 30 35 acc gaa ttg cac aag gga cag cga atc gat ttt gac tcc gcc gca gcc 259 Thr Glu Leu His Lys Gly Gln Arg He Asp Phe Asp Phe Wing Wing Gly 40 45 50 cgt aag ggc ect caa gca ctt cga cga ata aag att ctt gaa act cca cgc 307 Arg Lys Gly Pro Gln Ala Leu Arg He Lys He Leu Glu Thr Pro Arg 55 60 65"agg cgt cca cag cac aaa tac aag cca gaa gac ctc aac gga atg atc 355 Arg Arg Pro Gln His Lys Tyr Lys Pro Glu Glu Leu Asn Gly Met He 70 75 80 85 tct gac ctc atc acg ctt tc gaa agt gga gtg caa cca gcc ctt gcc 403 Ser Asp Leu He Thr Leu Leu Glu Ser Gly Val Gln Pro Gly L eu Wing 90 95 '100 aaa ggg caa tac ccg gag cac aaa gct gga gcg cag gta gca gaa att 451 Lys Gly Gln Tyr Pro Glu His Lys Ala Gly Ala Gln Val Ala Glu He 105 110 115 ctt cgc gtt gtt gcg aag gag ctt gag tct taaaacaata aggagaggat 501 Leu Arg Val Val Ala Lys Glu Leu Glu Ser 120 125 ccg 504 < 210 > 86 < 211 > 127 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 86 Val Pro Val Gly Thr Val Lys Trp Tyr Asp Ala Glu Arg Gly Phe Gly 1 5 10 15 Phe Val Ser Asn Pro Gly Gly Glu Asp Cys Phe Val Gly Lys Gln Val 20 25 30 Leu Pro Lys Gly Val Thr Glu Leu His Lys Gly Gln Arg He Asp Phe 35 40 • 45 Asp Phe Wing Wing Gly Arg Lys Gly Pro Gln Wing Leu Arg He Lys He 50 55 60 Leu Glu Thr Pro Arg Arg Arg Pro Gln His Lys Tyr Lys Pro Glu Glu 65 70 75 80 Leu Asn Gly Met He Be Asp Leu He Thr Leu Leu Glu Be Gly Val 85 90 95 Gln Pro Gly Leu Wing Lys Gly Gln Tyr Pro Glu His Lys Wing Ala Gly Wing 105 105 110 Gln Val Wing Glu He Leu Wing Val Val Wing Lys Glu Leu Glu Ser 115 120 125 < 210 > 87 < 211 > 324 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (301) < 223 > RXA00810 < 400 > 87 tcggcgttcg ttcaagaaac ggccagatgt tgctgttcga gctcatgcaa gagtggaaca 60 tcgacccgg tagtaattcc aatcagtaaa ggtaagacaa atg gca cag ggc act 115 Met Wing Gln Gly Thr 1 5 gtt aag tgg ttc aac cca gag aag ggc ttc ggc ttc atc gct ect tcc 163 Val Lys Trp Phe Asn Pro Glu Lys Gly Phe Gly Phe He Wing Pro Ser 10 15 20 gac gga tcc gct gac gtt ttc gtc cac tac tcc gag atc gag ggc aac 211 Asp Gly Ser Wing Asp Val Phe Val His Tyr Ser Glu He Glu Gly Asn 25 30 35 «ggc ttc cgt acc ctc gag gag aac cag ctc gtc gag ttc gaa atc ggc 259 Gly Phe Arg Thr Leu Glu Glu Asn Gln Leu Val Glu Phe Glu He Gly 40 45 50 gag ggc gct aag ggc ctt cag gct cag gct gtt cgt gca atc 301 Glu Gly Wing Lys Gly Leu Gln Wing Gln Wing Val Arg Wing He 55 60 65 taattgcatc tgagttcgaa acc 324 < 210 > 88 < 211 > 67 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 88 Met Wing Gln Gly Thr Val Lys Trp Phe Asn Pro Glu Lys Gly Phe Gly 1 5 10 15 5 Phe He Wing Pro Ser Asp Gly Ser Wing Asp Val Phé Val His Tyr Ser 20 25 30 Glu He Glu Gly Asn Gly Phe Arg Thr Leu Glu Glu Asn Gln Leu Val 35 40 '. 45 Glu Phe Glu He Gly Glu Gly Wing Lys Gly Leu Gln Wing Gln Wing Val 50 55 60 Arg Wing He 65 10 <; 210 > 89 < 211 > 1017 < 212 > DNA < 213 > Corynebacterium glutamicum • < 220 > < 221 > CDS < 222 > (101) .. (994) < 223 > RXA01674 < 400 > 89 15 cggcgtcgat tccagaaggt ttgtagacat gcttcaaggt tgcgctaatt gaaaagaacg 60 cggtagacgg tactttcata tccacccata taatgttgat atg gat aat ggg tgg 115 Met Asp Asn Gly Trp May 1 ccg aac ctg caa act ctc gca ctc ttt gtg gcg att gtg gaa gag ggg 163 Pro Asn Leu Gln Thr Leu Ala Leu Phe Val Wing He Val Glu Glu Gly 10 15 20 • age ctc ggt gcc ggt gct cga aaa gtc gga atg gcc caa ect aat gcc 211 Ser Leu Gly Ala Gly Ala Arg Lys Val Gly Met Ala Gln Pro Asn Ala 20 25 30 35 agt cgg gct atc gca gag ctt gag gca gac atg aaa gcc gaa ttg ttg 259 Be Arg Wing He Wing Glu Leu Glu Wing Asp Met Lys Wing Glu Leu Leu 40 45 50 gta cgt cat ect cga gga tea cat cca here gct gct gga ctt gcg ctt 307 Val Arg His Pro Arg Gly Ser His Pro Thr Wing Wing Gly Leu Wing Leu 55 60 65 gtt gag cat tcg cgc gat ctg ctt caa tct gta caa gaa ttt act gaa 355 Val Glu His Ser Arg Asp Leu Leu Gln Ser Val Gln Glu Phe Thr Glu 25 70 75 80 85 tgg gtg here gag gga cga act gag cag ccg ctg aaa ttg cat gtt ggg 403 Trp Val Thr Glu Gly Arg Thr Glu Gln Pro Leu Lys Leu His Val Gly 90 95 100 gcc agt atg acc att gcc gag gct cta cct cca cct gct tgg gtt gcg gac 451 Wing Ser Met Thr He Wing Glu Wing Leu Leu Pro Wing Trp Val Wing Asp 105 110 115 atg cgc acg cgt ttt ect gcc tgc cgt gtc gac gtc tct gtg atg aat 499 Met Arg Thr Arg Phe Pro Wing Cys Arg Val Asp Val Ser Val Met Asn 120 125 130 tct cact gta att gaa gcc gtc cag aaa ggg cac ttg caa cta ggt 547 Be Ser Gln Val He Glu Wing Val Gln Lys Gly His Leu Gln Leu Gly 135 140 145 ttt att gaa here ccg cat gtt ccc gta cgg ctt cat gct cgt gtg gtg 595 Phe He Glu Thr Pro His Val Pro Val Arg Leu His Ala Arg Val Val 150 155 160 165 caa gag gac aag ctg att gtg gtg att tct ect aat cat gag tgg gct 643 Gln Glu Asp Lys Leu He Val Val He Ser Pro Asn His Glu Trp Wing 170 175 180 aat cgc acg ggt agg atc agt ctt cgg gag ttg 'tcg gaa act ccg ctg 691 Asn Arg Thr Gly Arg He Ser Leu Arg Glu Leu Ser Glu Thr Pro Leu 185 190 195 ata gtg agg gaa gtc ggc tea ggt acc cga gaa gca tta caa gaa tta 739 He Val Arg Glu Val G and be Gly Thr Arg Glu Wing Leu Gln Glu Leu 200 205 210 ctt gcg gat tat gac atg gct gag ccg att caa gtg tta aac age aat 787 Leu Wing Asp Tyr Asp Met Wing Glu Pro He Gln Val Leu Asn Ser Asn 215 220 225 gct gcg gta cgt gtt gtt gtt gaa gca gca gca ggt ect gca gta ctt 835 Wing Wing Val Arg Val Val Glu Wing Gly Wing Gly Pro Wing Val Leu 230 235 240 245 ggt gaa tta gcc ttg cgt gat cat ctt gcg ctc ggc agg ctg ttg agt 883 Gly Glu Leu Wing Leu Arg Asp His Leu Wing Leu Gly Arg Leu Leu Ser 250 255 '260 gtg cca ttt gaa ggc agt gga gtt act cgt ect ctt here gct gtg tgg 931 Val Pro Phe Glu Gly Ser Gly Val Thr Arg Pro Leu Thr Wing Val Trp 265 270 275 agt gga ccc cgc aga ttg ccg att cta gcg gga gaa tta gtg tcc atc 979 Ser Gly Pro Arg Arg Leu Pro He Leu Wing Gly Glu Leu Val Ser He 280 285 290 gca tcg aac cac atc tgattttgag ccctggctaa cgg 1017 Ala Ser Asn His He 295 < 210 > 90 < 211 > 298 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 90 Met Asp Asn Gly Trp Pro Asn Leu Gln Thr Leu Ala Leu Phe Val Wing 5 1 5 10 15 He Val Glu Glu Gly Ser Leu Gly Wing Gly Wing Arg Lys Val Gly Met 20 25 30 Wing Gln Pro Asn Wing Being Arg Wing He Wing Glu Leu Glu Wing Asp Met 35 40 45 Lys Wing Glu Leu Leu Val Arg His Pro Arg Gly Ser His Pro Thr Wing 50 55 60 Wing Gly Leu Wing Leu Val Glu His Ser Arg Asp Leu Leu Gln Ser Val 10 65 70 75 80 Gln Glu Phe Thr Glu Trp Val Thr Glu Gly Arg Thr Glu Gln Pro Leu 85 90 95 Lys Leu His Val Gly Ala Ser Met Thr He Ala Glu Ala Leu Leu Pro 100 105 110 Wing Trp Val Wing Asp Met Arg Thr Arg Phe Pro Wing Cys Arg Val Asp 115 120 125 Val Ser Val Met Asn Ser Ser Gln Val He Glu Ala Val Gln Lys Gly 15 130 135 140 His Leu Gln Leu Gly Phe He Glu Thr Pro His Val Pro Val Arg Leu 145 150 155. 160 His Wing Arg Val Val Gln Glu Asp Lys Leu He Val Val He Ser Pro 165 170 175 Asn His Glu Trp Wing Asn Arg Thr Gly Arg He Ser Leu Arg Glu Leu 180 185 190 Ser Glu Thr Pro Leu He Val Arg Glu Val Gly Ser Gly Thr Arg Glu 20 195 200 • 205 Wing Leu Gln Glu Leu Leu Wing Asp Tyr Asp Met Wing Glu Pro He Gln 210 215 220 Val Leu Asn Ser Asn Wing Wing Val Arg Val Val Val Glu Wing Gly Wing 225 230 235 240 Gly Pro Ala Val Leu Gly Glu Leu Ala Leu Arg Asp His Leu Ala Leu 245 250 255 Gly Arg Leu Leu Ser Val Pro Phe Glu Gly Ser Gly Val Thr Arg Pro 25 260 265 270 ^^ g ^ j ^ a Leu Thr Wing Val Trp Ser Gly Pro Arg Arg Leu Pro He Leu Wing Gly 275 280"285 Glu Leu Val Ser He Wing Being Asn His He 290 295 < 210 > 91 < 211 > 1214 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (1191) < 223 > RXA02431 < 400 > 91 gtg gtg gtg here ccc cgt cat atc gtt tac tcc gca gcc tcg cgc cgg 48 Val Val Val Thr Pro Arg His He Val Tyr Ser Ala Ala Ser Arg Arg 1 5 10 15 gtg ttc caa atc gtg gaa aaa cgc gcc gga att gtc gaa cgc ctc age 96 Val Phe Gln He Val Glu Lys Arg Ala Gly He Val Glu Arg Leu Ser 20 25 30 atc gat gaa ggc ttc atg gaa cca gag gct ctc gtt gga gcc cca acc 144 He Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Ala Thr Pro 35 40 45 gaa gag gtg aaa cag tgg gcg gaa gata tta cgc gcg gaa att aaa gaa 192 Glu Glu Val Lys Gln Trp Ala Glu Glu Leu Arg Wing Glu He Lys Glu 50 55 60 gtt act ggc tta ccc tcc tcg gtt ggt gct ggc tcc ggt aag cag atc 240 Val Thr Gly Leu Pro Ser Ser Val Gly Wing Gly Ser Gly Lys Gln He 65 70 75 80 gcc aaa att ggt tea ggc gaa gca aag cca gat ggt gtg ttt gtc gtg 288 Wing Lys He Gly Ser Gly Glu Wing Lys Pro Asp Gly Val Phe Val Val 85 90 95 cca gta gac aag caa cat gac ttg ctt gat cca ctt ect gtg ggc gca 336 Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val Gly Wing 100 105 110 ctt tgg gga gtg ggt ect gtg here ggc tcc aag ctt gcc tea atg ggg 384 Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser Met Gly 115 12 0 125 gtg gaa here att ggt gat cta gca gcg cta acc aaa gaa gta gaa 432 Val Glu Thr He Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu Val Glu 130 135 140 atc age ctc ggt gca acc atc gga ata tea ctg tgg aac ctt gcc cga 480 He Ser Leu Gly Wing Thr He Gly He Ser Leu Trp Asn Leu Wing Arg 145 150 155"160 gga atc gac gac cgc ect gtg gaa ccc cgc gcc gaa gca aaa cag atc 528 Gly He Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys Gln He 165 170 '175 tcc caa gag cac acc tat gaa aaa gac ctc ctc acc agg caa caa gta 576 Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln Gln Val 180 185 190 gat gct gcc atc att cga tea gcc gaa ggc gca cac cga cgg ctc ctc 624 Asp Ala Ala He He Arg Be Ala Glu Gly Ala His Arg Arg Leu Leu 195 200 205 aaa gac ggc ggt ggt gcc aga act gtc age gtg aaa ctg cgg atg gcc 672 Lys Asp Gly Arg Gly Wing Arg Thr Val Ser Val Lys Leu Arg Met Wing 210 215 220 gac ttt cgt att gag tct cgt tcc tac acc ttg tcc tat gcc acc gat 720 Asp Phe Arg He Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala Thr Asp 225 230 235 240 gat tac gca act ctt gag gca here gca ttc cga ctt gcc cgc tac ccc 768 Asp Tyr Ala Thr Leu Glu Ala Thr Ala Phe Arg Leu Ala Arg Tyr Pro 245 250 255 gga gaa gta ggc ccc atc cgc ctt gtc gga gta a, gt ttt tct ggt ttg 816 Gly Glu Val Gly Pro He Arg Leu Val Gly Val Ser Phe Ser Gly Leu 260 265 270 gaa gaa tcc cgc caa gac atc ctc tcc ccg gaa ctt gac caa caa atc 864 Glu Glu Ser Arg Gln Asp He Leu Phe Pro Glu Leu Asp Gln Gln He 275 280 285 atc gta cca cca gca ccc gac acc gat tat gag gta ggc gtg caa tcc 912 He Pro Val Pro Pro Wing Asp Thr Asp Tyr Glu Val Gly Val Gln Ser 290 295 300 tct tct agt tcc gaa agt act ca gtt gaa gcg ccg caa gat gtc gcg 960 Being Ser Glu Being Thr Gln Val Glu Wing But Gln Asp Val Ala 305 310 315 320 ttg agt atg tgg tgc gca acg caa gat gtc tac cac cca gaa tat ggc 1008 Leu Ser Met Trp Cys Wing Thr Gln Asp Val Tyr His Pro Glu Tyr Gly 325 330 335 cac ggt tgg gta ca ggt gcc ggt cac ggt gtt gta gta tea gta cgt ttt 1056 His Gly Trp Val Gln Gly Wing Gly His Gly Val Val Ser Val Arg Phe 340 345 '350 gaa acc cgc age acc aaa ggg cga act aaa agt ttt tcc atg gat 1104 Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser Met Asp 355 360' 365 gac ccg gac ctc ac ccg gca gac ect cta gat agt ttg gat tgg ggg 1152 Asp Pro Asp Leu Thr Pro Wing Asp Pro Leu Asp Ser Leu Asp Trp Wing 370 375 380 gac tgg ttt gct gaa aat ggt gaa acg ggg gat gac gaa tagggtttca 1201 Asp Trp Phe Ala Glu Asn Gly Glu Thr Gly Asp Asp Glu 385 390 395 tcgggtttcg ggg 1214 < 210 > 92 < 211 > 397 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 92 Val Val Val Thr Pro Arg His He Val Tyr Ser Ala Ala Ser Arg Arg 1 5 10 15 Val Phe Gln He Val Glu Lys Arg Ala Gly He Val Glu Arg Leu Ser 20 25 30 He Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Wing Thr Pro 35 40 45 Glu Glu Val Lys Gln Trp Wing Glu Glu Leu Arg Wing Glu He Lys Glu 50 55 60 Val Thr Gly Leu Pro Ser Ser Val Gly Wing Gly Ser Gly Lys Gln He 65 70 75 80 Wing Lys He Gly Ser Gly Glu Wing Lys Pro Asp Gly Val Phe Val Val 85 90 95 Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val Gly Wing 100 105. 110 Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser Met Gly 115 120 125 Val Glu Thr He Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu Val Glu 130 135 140 He Ser Leu Gly Ala Thr He Gly He Ser Leu Trp Asn Leu Wing Arg 145 150 155 160 Gly He Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys Gln He 165 170 • 175 Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln Gln Val 180 185 190 Asp Ala Wing He He Arg Wing Glu Wing Gly Wing His Arg Arg Leu Leu 195 200 205 Lys Asp Gly Arg Gly Wing Arg Thr Val Ser Val Lys Leu Arg Met Wing 210 215 220 Asp Phe Arg He Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala Thr Asp 225 230 235 240 Asp Tyr Wing Thr Leu Glu Wing Thr Wing Phe Arg Leu Wing Arg Tyr Pro 245 250 255 Gly Glu Val Gly Pro He Arg Leu Val Gly Val Ser Phe Ser Gly Leu 260 265 270 Glu Glu Ser Arg Gln Asp He Leu Phe Pro Glu Leu Asp Gln Gln He 275 280 285 He Val Pro Pro Wing Pro Asp Thr Asp Tyr Glu Val Gly Val Gln Ser 290 295 300 Ser Ser Be Glu Ser Thr Gln Val Glu Ala Pro Gln Asp Val Ala 305 310 315 320 Leu Ser Met Trp Cys Wing Thr Gln Asp Val Tyr His Pro Glu Tyr Gly 325 330 335 His Gly Trp Val Gln Gly Wing Gly His Gly Val Val Ser Val Arg Phe 340 345 350 Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser Met Asp 355 360 365 Asp Pro Asp Leu Thr Pro Wing Asp Pro Leu Asp Ser Leu Asp Trp Wing 370 375 380 Asp Trp Phe Wing Glu Asn Gly Glu Thr Gly Asp Asp Glu 385 390 395 < 210 > 93 < 211 > 558 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (535) < 223 > RXA02446 < 400 > 93 caggaaatct tgctgtctaa tgcacaacgg aaaactgctc acggacgaac tagttgtagc 60 gagcgtattc tttgtgttct ctcacgacag gaatactgct atg gcg atc gag tcc 115 Met Ala He Glu Ser 1 5 atc gcg tac acc agt gaa gca ctc tea acc ggc agt ggc cgg ctg ggg 163 He Ala Tyr Thr Ser Glu Ala Leu Be Thr Gly Be Gly Arg Leu Gly 10 15 20 cat gtg cgc tcc here gat ggt gcg ctc gaa ttt gaa atg here ccg cca 211 His Val Arg Ser Thr Asp Gly Ala Leu Glu Phe Glu Met Thr Pro Pro 25 30 35 aag gct ttg ggc gga tcc ggt gaa ggc acc aat cca gaa cag ctg ttc 259 Lys Ala Leu Gly Gly Ser Gly Glu Gly Thr Asn Pro Glu Gln Leu Phe 40 45 50 gcg gta ggt tac gca gcc tgt tcc cac tct gcc atg cac tct gtc gca gca 307 Wing Val Gly Tyr Wing Wing Cys Phe His Ser Wing Met His Ser Val Wing 55 60 65 cgc age cgc aag atc act ctt gaa gac here gcg gtt ggt gcc cga gtt 355 Arg Ser Arg Lys He Thr Leu Glu Asp Thr Wing Val Gly Wing Arg Val 70 75 80. ' 85 age atc ggg cca aac ggc gct ggt gga ttt gag att gcc gta gaa ctc 403 Ser He Gly Pro Asn Gly Wing Gly Gly Phe Glu He Wing Val Glu Leu 90 95 '100 gaa gta tcg att ect caa ttg cca caa gca gaa gcc cag gaa ctt gct 451 Glu Val Ser He Pro Gln Leu Pro Gln Ala Glu Ala Gln Glu Leu Ala 105 110 115 gat gcc gcg cac cag gtg tgc ccg tat tcc aac gcc here cg ggc aat 499 Asp Ala Ala His Gln Val Cys Pro Tyr Ser Asn Ala Thr Arg Gly Asn 120 125 130 -atc tcg gta act gtg tea gtc atc gac gaa gag gct taaaaccaca 545 He Ser Val Thr Val Ser Val He Asp Glu Glu Ala 135 140 145 ggattaacaa aac 558 < 210 > 94 < 211 > 145 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 94 Met Wing He Glu Ser Wing Tyr Thr Ser Glu Wing Leu Ser Thr Gly 1 5 10 15 Ser Gly Arg Leu Gly His Val Arg Ser Thr Asp Gly Ala Leu Glu Phe 20 25 '30 Glu Met Thr Pro Pro Lys Ala Leu Gly Gly Ser Gly Glu Gly Thr Asn 35 40 45 Pro Glu Gln Leu Phe Wing Val Gly Tyr Wing Wing Cys Phe His Ser Wing 50 55 60 Met His Ser Val Wing Arg Ser Arg Lys He Thr Leu Glu Asp Thr Wing 65 70 75. 80 Val Gly Wing Arg Val Ser He Gly Pro Asn Gly Wing Gly Gly Phe Glu 85 90 • 95 He Wing Val Glu Leu Glu Val Ser He Pro Gln Leu Pro Gln Wing Glu 100 105 110 Wing Gln Glu Leu Wing Asp Wing Wing His Gln Val Cys Pro Tyr Ser Asn 115 120 125 Wing Thr Arg Gly Asn He Ser Val Thr Val Ser Val He Asp Glu Glu 130 135 140 Wing 145 < 210 > 95 < 211 > 1206 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1183) 10 < 223 > RXA02861 < 400 > 95 ccctcgatta ttacaatccg gctacttcgg aagcgtttgt ggccggattt ttctatcctg 60 • gaaggtatta atacttcttt aagggtcgga ggattttcgt atg tct act aga here 115 Met Ser Thr Arg Thr 1 5 acg cca caa gac cgt tat acc gac gaa tac ggc atc gaa cgc gtc aac 163 Thr Pro Gln Asp Arg Tyr Thr Asp Glu Tyr Gly He Glu Arg Val Asn 10 15 20 15 aag gat gaa ccc ggc ctg gtg gac aaa ctc cgg gac aag cac gac tgg 211 Lys Asp Glu Pro Gly Leu Val Asp Lys Leu Arg Asp Lys His Asp Trp 25 30 35 ttt gat cat ctc atg cgc atg aat gaa cgt ttc ggc gca aaa ggt ggc 259 Phe Asp His Leu Met Arg Met Asn Glu Arg Phe Gly Wing Lys Gly Gly 40 45 50 • aac caa ttg tcg gcg ggt att acg tat ttc tcc gtg ctg tcg atc ttc 307 Asn Gln Leu Ser Ala Gly He Thr Tyr Phe Ser Val Leu Ser He Phe 55 60 .65 20 ccg att gcc atg ctt gtc ttc ggt att gca ggt gtc atc ctt gcc gga 355 Pro He Wing Met Leu Val Phe Gly He Wing Gly Val He Leu Wing Gly 70 75 80 85 aac ect gaa gtt ctc here gat a tta c aat cga atc aac a g aac g tta 403 Asn Pro Glu Val Leu Thr Asp He Gln Asn Arg He Asn Asp Ala Leu 90 95 100 gaa ggc gag atc ggt aac acc gt c aac ggc atc att gat ccc gcg att 451 Glu Gly Glu He Gly Asn Thr Val Asn Gly He He Asp Be Wing He 105 110 115 25 gcg cag cgt ggt gct gtg gt ggt ggt gta gta gta act gcc ctg tgg 499 Wing Gln Arg Gly Wing Val Leu Gly He Gly Gly Val Thr Wing Leu Trp 120 125 130 tct gga ctg ggg tgg atg gcg aac ctg cgc ttt gga gtcc ccc tg cgc atg 547 Ser Gly Leu Gly Trp Met Wing Asn Leu Arg Phe Gly Val Ser Arg Met 135 140 145 5 tgg gcc att gac cca act gaa ggc aac ttc att caa aag aag ctc acc 595 • Trp Ala He Asp Pro Thr Glu Gly Asn Phe He Gln Lys Lys Leu Thr 150 155 160 165 gac ttg gtc gcg ctg atc gtc ttg ctg ctg gcc atg ggc gta gcc ttc 643 Asp Leu Val Ala Leu He Val Leu Leu Leu Ala Met Gly Val Ala Phe 170 175 180 ggt atc acg gcg ctc ggt gct tcc gga cta acc aaa aac ctg ctg gac 691 Gly He Thr Ala Leu Gly Ala Ser Gly Leu Thr Lys Asn Leu Leu Asp 185 190 195 10 ttt gtg ggc ctg ggg gag att ccg ggc att age tac atc acc tgg gtg 739 Phe Val Gly Leu Gly Glu He Pro Gly He Ser Tyr He Thr Trp Val 200 205 210 gtc gca gca ctt gtt ggt gtc ttg gct aac ttc ctg gtg ttc atg tgg 787 Val Ala Ala Leu Val Gly Val Leu Wing Asn Phe Leu Val Phe Met Trp 215 220 225 ctg att ttc tcc ctg cca cgt acc aaa gtt ccc atg aaa ccg ggt ctt 835 Leu He Phe Ser Leu Pro Arg Thr Lys Val Pro Met Lys Pro Gly Leu 230 235 240 245 15 cag gca gca ctg ctt ggc gca atc ggt ttt gag gtg gtc aag cag gtt 883 Gln Ala Ala Leu Leu Gly Ala He Gly Phe Glu Val Val Lys Gln Val 250 255 260 gga tcg ctg ttg gct tea aat gca ttg agt aac ccc gcg ggt gca gca gca gca 931 Gly Ser Leu Leu Ala Ser Asn Ala Leu Ser Asn Pro Ala Gly Ala Ala 265 270 275 ttc ggt ccg atc atc ggc atc atg gtt gtg ctg tat ttg atc tgg cgc 979 Phe Gly Pro He He Gly He Met Val Val Leu Tyr Leu He Trp Arg 280 285 290 20 ate ctc atg tat tgc tct gcg tgg gct gcc acc agt gaa gac gcg ttg 1027 He Leu Met Tyr Cys Ser Ala Trp Ala Ala Thr Ser Glu Glu Ala Leu 295 300 30'5 cgt ctt gcg act gtt cca gca cca gag ect gcg atc att cgg gtt cgc 1075 Arg Leu Wing Thr Val Pro Wing Pro Glu Pro Wing He He Arg Val Arg 310 315 320 325 cat gaa att gat cca ggt gaa gaa gtc tcc caa tct gct cga aaa gtg 1123 His Glu He Asp Pro Gly Glu Glu Val Ser Gln Ser Wing Arg Lys Val 330 335 * 340 25 ggc att gga gtg gcc gtg ggt gcc gcg act gcg ggt gct ttt gcg ctg 1171 Gly He Gly Val Wing Val Gly Ala Wing Thr Ala Gly Ala Phe Ala Leu 345 350 355 ttg cgt aaa aaa tagtttttat taagggcatt ccc 1206 Leu Arg Lys Lys 360 < 210 > 96 < 211 > 361 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 96 Met Ser Thr Arg Thr Thr Pro Gln Asp Arg Tyr Thr Asp Glu Tyr Gly 1 5 10 15 He Glu Arg Val Asn Lys Asp Glu Pro Gly Leu Val Asp Lys Leu Arg 20 25 30 Asp Lys His Asp Trp Phe Asp His Leu Met Arg Met Asn Glu Arg Phe 35 40 45 Gly Wing Lys Gly Gly Asn Gln Leu Ser Wing Gly He Thr Tyr Phe Ser 50 55 '60 Val Leu Ser He Phe Pro He Wing Met Leu Val Phe Gly He Wing Gly 65 70 75. 80 Val He Leu Wing Gly Asn Pro Glu Val Leu Thr Asp He Gln Asn Arg 85 90 95 He Asn Asp Wing Leu Glu Gly Glu He Gly Asn Thr Val Asn Gly He 100 105 110 He Asp Be Wing He Wing Gln Arg Gly Wing Val Leu Gly He Gly Gly 115 120 125 Val Thr Ala Leu Trp Ser Gly Leu Gly Trp Met Wing Asn Leu Arg Phe 130 135 .'140 Gly Val Ser Arg Met Trp Wing He Asp Pro Thr Glu Gly Asn Phe He 145 150 155 160 Gln Lys Lys Leu Thr Asp Leu Val Wing Leu He Val Leu Leu Leu Wing 165 170 175 Met Gly Val Wing Phe Gly He Thr Wing Leu Gly Wing Ser Gly Leu Thr 180 185 190 Lys Asn Leu Leu Asp Phe Val Gly Leu Gly Glu He Pro Gly He Ser 195 200 205 Tyr He Thr Trp Val Val Ala Wing Leu Val Gly Val Leu Wing Asn Phe 210 215 220 Leu Val Phe Met Trp Leu He Phe Ser Leu Pro Arg Thr Lys Val Pro 225 230 235 240 Met Lys Pro Gly Leu Gln Ala Ala Leu Leu Gly Ala He Gly Phe Glu 245 250 255 Val Val Lys Gln Val Gly Ser Leu Leu Ala Ser Asn Ala Leu Ser Asn 260 265 270 Pro Ala Gly Ala Ala Phe Gly Pro He He Gly He Met Val Val Leu 275 280 285 Tyr Leu He Trp Arg He Leu Met Tyr Cys Ser Wing Trp Wing Wing Thr 290 295 300 Ser Glu Glu Wing Leu Arg Leu Wing Thr Val Pro Wing Pro Glu Pro Wing 305 310 315 320 He He Arg Val Arg His Glu He Asp Pro Gly Glu Glu Val Ser Gln 325 330 335 Be Wing Arg Lys Val Gly He Gly Val Wing Val Gly Wing Wing Thr Wing 340 345 350 Gly Wing Phe Wing Leu Leu Arg Lys Lys 355 360 < 210 > 97 < 211 > 753 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (730) < 223 > RXA00981 < 400 > 97 aaccaatggc tgggtactga tgtggtgatc agtgcccagt ttcttctttc tactagtgtc 60 ggatagaagt acccccagtc cagaatgaag gtcaccacca atg tea gag aat ttg 115 Met. Ser Glu Asn Leu 1 5 cea gcg ccc gag aat ctc ctg gac gcc gag aga att cag atg atc aag 163 Pro Wing Pro Glu Asn Leu Leu Asp Wing Glu Arg He Gln Met He Lys 10 15. 20 aac tcc aac gaa tta acg ggg ttc atg ctc aac tac cac ttt ggc 211 Asn Phe Arg Asn Glu Leu Thr Gly Phe Met Leu Asn Tyr Gln Phe Gly 25 30 '35 att gat gag atc ctg acc aag atc aac atc ctg aaa act gaa ttc age 259 He Asp Glu He Leu Thr Lys He Asn He Leu Lys Thr Glu Phe Ser 40 45 50 cag ctg cac gaa tac gca ect atc gag cac gta tct tea cga ttg aag 307 Gln Leu His Glu Tyr Ala Pro He Glu His Val Being Ser Arg Leu Lys 55 60 65 here cca gaa age atc gtc aaa aag gtc atc cga aaa gga gac gag ctc 355 Thr Pro Glu Ser He Val Val Lys Val He Arg Lar Gly Asp Glu Leu 70 75 80 '85 tcc ctc gca gct atc aaa gac here gtg ttt gat atc gca ggc att cga 403 Ser Leu Ala Ala He Lys Asp Thr Val Phe Asp He Wing Gly He Arg 90 95 100 atc gtc tgc agt ttc ctc aaa gat gcc tac gca atc gcc gat atg ctg 451 He Val Cys Ser Phe Leu Lys Asp Wing Tyr Wing He Wing Asp Met Leu 105 110 115 ac aac aaa gac gtc acg gtc atc gag gcc aaa gac tac atc gct 499 Thr Asn Gln Lys Asp Val Thr Val He Glu Wing Lys Asp Tyr He Wing 120 125 130 aac cca aag ccg aac ggc tac aag agt ttg cac ctt atc ctc caa gtg 547 Asn Pro Lys Pro Asn Gly Tyr Lys Ser Leu His Leu He Leu Gln Val 135 140 145 ect gtc ttc ctg tct aac tcc gtg gaa aag gtc aat gtt gaa gtc cag 595 Pro Val Phe Leu Ser Asn Ser Val Glu Lys Val Asn Val Glu Val Gln 150 155 160! 165 atc cgc acc att gcc atg gac ttc tgg gca age ctc gag cac aaa atc 643 He Arg Thr He Wing Met Asp Phe Trp Wing Being Leu Glu His Lys He 170 175 180 tac tac aaa ttt gaa caa gaa gtt ect cag ate ate ctt gat gag ctc 691 Tyr Tyr Lys Phe Glu Gln Glu Val Pro Gln Ser He Leu Asp Glu Leu 185 190 195 agt gaa gat gga aag aat cca cgg gga agt gaa gtc act taaacctcca 740 Ser Glu Asp Gly Lys Asn Pro Arg Gly Ser Glu Val Thr 200 205. 210 gttgaaacca ctg 753 < 210 > 98 < 211 > 210 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 98 Met Ser Glu Asn Leu Pro Wing Pro Glu Asn Leu Leu Asp Wing Glu Arg 1 5 10 15 He Gln Met He Lys Asn Phe Arg Asn Glu Leu Thr Gly Phe Met Leu 20 25 30 Asn Tyr Gln Phe Gly He Asp Glu He Leu Thr Lys He Asn He Leu 35 40 45 Lys Thr Glu Phe Ser Gln Leu His Glu Tyr Ala Pro He Glu His Val 50 55 60 Ser Ser Arg Leu Lys Thr Pro Glu Ser He Val Lys Lys Val He Arg 65 70 75 _. 80 Lys Gly Asp Glu Leu Ser Leu Wing Wing He Lys Asp Thr Val Phe Asp 85 90 95 • He Wing Gly He Arg He Val Cys Ser Phe Leu Lys Asp Wing Tyr Wing 100 105 110 He Wing Asp Met Leu Thr Asn Gln Lys Asp Val Thr Val He Glu Wing 115 120 125 Lys Asp Tyr He Wing Asn Pro Lys Pro Asn Gly Tyr Lys Ser Leu His 130 135 140 Leu He Leu Gln Val Pro Val Phe Leu Ser Asn Ser Val Glu Lys Val 145 150 155 160 10 Asn Val Glu Val Gln He Arg Thr He Wing Met Asp Phe Trp Wing Ser 165 170 175 Leu Glu His Lys He Tyr Tyr Lys Phe Glu Gln Glu Val Pro Gln Ser 180 185 190 He Leu Asp Glu Leu Ser Glu Asp Gly Lys Asn Pro Arg Gly Ser Glu 195 200 • 205 Val Thr 210 15 < 210 > 99 < 211 > 1098 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1075) < 223 > RXN00786 20 < 400 > 99 tccttcttcc accagctaaa agtgaattac cccgcctttg tcggggtggt tgcattccca 60 gtctaggtgt ttagcctcaa cgttggatac gctgggaggc atg age tea ect gtt 115 Met Ser Ser Pro Val 1 5 atc age ccc gaa acc aaa acc gga aag aag atc ctg ctt gca gcc ect 163 He Ser Pro Glu Thr Lys Thr Gly Lys Lys Leu Leu Wing Ala Pro 10 15 20 cgc gga tac tgt gcc ggc gta gac cgt gca gtg gaa acc gtc gag cgc 211 25 Arg Gly Tyr Cys Wing Gly Val Asp Arg Wing Val Glu Thr Val Glu Arg 25 30 35 gcg ctc gag gaa tac ggc gcc cca att tat gtc cgt aaa gaa atc gtg 259 Wing Leu Glu Glu Tyr Gly Wing Pro He Tyr Val Arg Lys Glu He Val 40 45 50 cac aac cgt tac gtt gtg gac acc ctg gca gaa aag ggc gcg att ttt 307 His Asn Arg Tyr Val Val Asp Thr Leu Wing Glu Lys Gly Wing He Phe 55 60, 65 gtc aac gaa gca tct gaa gca cca gaa ggt gcc aac atg gtg ttc tct 355 Val Asn Glu Wing Ser Glu Wing Pro Glu Gly Wing Asn Met Val Phe Ser 70 75 80 85 gca cac ggc gtg age cca atg gtc cac gaa gaa gct gca gct aaa aac 403 Wing His Gly Val Ser Pro Met Val His Glu Glu Wing Wing Wing Lys Asn 90 95 100 atc aag gct att gac gcg gcc tgc ccg ctg gtc acc aaa gtg cac aag 451 He Lys Ala He Asp Ala Ala Cys Pro Leu Val Thr Lys Val His Lys 105 110 115 gaa gtc cag cgc ttt gat aag cag gga ttc cac att ctc ttc atc ggt 499 Glu Val Gln Arg Phe Asp Lys Gln Gly Phe His He Leu Phe He Gly 120 125 130 cac gaa ggc cat gaa gaa gta gag ggc acc atg ggt cat tcc gtt gag 547 His Glu Gly His Glu Glu Val Glu Gly Thr Met Gly His Ser Val Glu 135 140 145 aaa acc cac ctg gtt gac ggc gtt gct ggc att gcc acc ctg ect gaa 595 Lys Thr His Leu Val Asp Gly Val Wing Gly He Wing Thr Leu Pro Glu 150 155 160 165 ttc tta aac gat gaa cca aac ctg atc tgg ctg tct cag acc acg ctt 643 Phe Leu Asn Asp Glu Pro Asn Leu He Trp Leu Ser Gln Thr Thr Leu 170 175 180 tct gtg gac gag acc atg gag atc gtc cgc gag ctg- aag gtg aag ttc 691 Ser Val Asp Glu Thr Met Glu He Val Arg Glu Leu Lys Val Lys Phe 185 190 '195 ect cag ctg cag gat cc g tea gat gat tgc tac gcc acg cag 739 Pro Gln Leu Gln Asp Pro Pro Ser Asp Asp He Cys Tyr Ala Thr Gln 200 205' 210 aac cgc cag gtt gcc gtc aag gct atc gct gag cgc tgc gag ctg atg 787 Asn Arg Gln Val Wing Val Lys Wing Wing Glu Arg Cys Glu Leu Met 215 220 225 att gtg gtc ggt tcc cgc aac tcc tcc aac tcg gtt cgt ctg gtt gag 835 He Val Val Gly Ser Arg Asn Ser Ser Asn Ser Val Arg Leu Val Glu 230 235 240 245 gtc gct aac gc aac ggt gcc gat aac gcc tac ctg gtg gat tac gcc 883 Val Ala Lys Gln Asn Gly Wing Asp Asn Wing Tyr Leu Val Asp Tyr Wing 250 255 260 cgc gaa atc gac cca gca tgg tcc gaa ggc gta gag ccc atc ggt atc 931 Arg Glu He Asp Pro Wing Trp Phe Glu Gly Val Glu Thr He Gly He 265 270 275 tcc tcc ggc gct tcc gtg ect gag atc ctc gtc cag ggc gtc att gag 979 Ser Ser Gly Wing Ser Val Pro Glu He Leu Val Gln Gly Val He Glu 280 285 290 cgc ctg gct gag ttc ggc tac gac gac gtc gag gaa gtc acc tcc gcc 1027 Arg Leu Wing Glu Phe Gly Tyr Asp Asp Goes l Glu Glu Val Thr Ser Wing 295 300 305 gct gag aag att gtt ttc gcg ctg ect cgc gtg ctg cgc cac aag aat 1075 Wing Glu Lys He Val Phe Wing Leu Pro Arg Val Leu Arg His Lys Asn 310 315 320 325 taattgcaag aatgaaaaat ccc 1098 10 < 210 > 100 < 211 > 325 < 212 > PRT < 213 > Corynebacterium glutamicum • < 400 > 100 Met Ser Ser Val Pro Ser Glu Thr Lys Thr Gly Lys Lys He 1 5 10 _ "15 Leu Leu Ala Wing Pro Arg Gly Tyr Cys Wing Gly Val Asp Arg Wing Val 20 25 * 30 15 Glu Thr Val Glu Arg Ala Leu Glu Glu Tyr Gly Ala Pro He Tyr Val 35 40 45 Arg Lys Glu He Val His Asn Arg Tyr Val Val Asp Thr Leu Ala Glu 50 55 60 Lys Gly Wing He Phe Val Asn Glu Wing Ser Glu Wing Pro Glu Gly Wing 65 70 75 _ 80 • Asn Met Val Phe Ser Wing His Gly Val Ser Pro Met Val His Glu Glu 85 90 95 20 Ala Ala Ala Lys Asn He Lys Ala He Asp Ala Ala Cys Pro Leu Val 100 105 110 Thr Lys Val His Lys Glu Val Gln Arg Phe Asp Lys Gln Gly Phe His 115 120 125 He Leu Phe He Gly His Glu Gly His Glu Glu Val Glu Gly Thr Met 130 135 140 Gly His Ser Val Glu Lys Thr His Leu Val Asp Gly Val Ala Gly He 145 150 155 160 25 Wing Thr Leu Pro Glu Phe Leu Asn Asp Glu Pro Asn Leu He Trp Leu - • '8y? «« «^ A 165 170' 175 Ser Gln Thr Thr Leu Ser Val Asp Glu Thr Met Glu He Val Arg Glu 180 185 • 190 Leu Lys Val Lys Phe Pro Gln Leu Gln Asp Pro Pro Ser Asp Asp He 195 200 205 Cys Tyr Ala Thr Gln Asn Arg Gln Val Wing Val Lys Wing Wing Glu 210 215 220 Arg Cys Glu Leu Met He Val Val Gly Ser Arg Asn Ser Ser Asn Ser 225 230 235 240 Val Arg Leu Val Glu Val Wing Lys Gln Asn Gly Wing Asp Asn Wing Tyr 245 250 255 Leu Val Asp Tyr Wing Arg Glu He Asp Pro Wing Trp Phe Glu Gly Val 260 265. 270 Glu Thr He Gly He Ser Ser Gly Wing Ser Val Pro Glu He Leu Val 275 280 285 Gln Gly Val He Glu Arg Leu Wing Glu Phe Gly Tyr Asp Asp Val Glu 290 295 300 Glu Val Thr Ser Wing Wing Glu Lys He Val Phe Ala Leu Pro Arg Val 305 310 315 320 Leu Arg His Lys Asn 325 < 210 > 101 < 211 > 1131 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1108) < 223 > RXS01027 < 400 > 101 aatagatgga agtagttttt cattcactta tgtgcgcgtt tttaatctgg tttctaccaa 60 gaactgtgtg caccacaacg cggaaggtga atcgcaccca atg gca aat aag aac 115 Met Wing Asn Lys Asn 1 5 aat aag ect cat gag gtg gac aaa gac caa gat tea gcc atg ctg atc 163 Asn Lys Pro His Glu Val Asp Lys Asp Gln Asp Ser Wing Met Leu He 10 15 20 aac ggt cgc ctg caa cag atc ccg gcg cgt ccc act gag gaa ttc acc 211 Asn Gly Arg Leu Gln Gln He Pro Wing Arg Pro Thr Glu Glu Phe Thr 25 30. 35 cgc ccac cct gc gcac gcac gc gc gc cgc cgc gcc gac atc acc 259 Arg Pro Thr Leu Wing Wing Gly Wing Val Leu Trp Arg Gly Asp He Thr 40 45 50 aac ccg gac age atc gag gtc gctc gc atc ccc ccc gc cac tat gat 307 Asn Pro Asp Ser He Glu Val Wing Val He His Arg Pro His Tyr Asp 55 60 65 gac tgg tcc ctg gcc aag ggc aaa gtc gat ccc ggc gag tct att ccg 355 Asp Trp Ser Leu Ala Lys Gly Lys Val Asp Pro Gly Glu Ser He Pro 70 75 80 85 here ac gcg gcc cgt gaa atc ctt gaa gaa act ggc tac gac atc cgt 403 Thr Thr Wing Wing Arg Glu He Leu Glu Glu Thr Gly Tyr Asp He Arg 90 95 100 ctg ggc aag ctg atc ggc aag gtt act tac ect gtg ctc gac cga acc 451 Leu Gly Lys Leu He Gly Lys Val Thr Tyr Pro Val Leu Asp Arg Thr 105 110 115 aaa gtg gtc tac tac tgg act gcc cag gtt ctt ggt gga gag ttt gtc 499 Lys Val Val Tyr Tyr Trp Thr Ala Gln Val Leu Gly Gly Glu Phe Val 120 125 • 130 ccc aac gat gaa gtt gat gaa atc cgt tgg ctg tct gtt gat gaa gca 547 Pro Asn Asp Glu Val Asp Glu He Arg Trp Leu Ser Val Asp Glu Ala 135 14 0 145 tgc gag ttg ctc age tac caa gta gat acc gaa gtt ctg gcc aag gca 595 Cys Glu Leu Leu Ser Tyr Gln Val Asp Thr Glu Va.l Leu Ala Lys Ala 150 155 160 165 gca aag cgt ttc cgc act ect tcc acc act cgg gtg ctg tat gtt cgc 643 Wing Lys Arg Phe Arg Thr Pro Ser Thr Thr Arg Val Leu Tyr Val Arg 170 175 180 cat gct cat gca cat ggt cgc caa acc tgg ggt ggc gac gac aat aag 691 His Wing His Wing His Gly Arg Gln Thr Trp Gly Gly Asp Asp Asn Lys 185 190 195 cgc cca ttg gac aaa aag ggg cgt cga caa gca gaa atg ctc gta ccc 739 Arg Pro Leu Asp Lys Lys Gly Arg Arg Gln Wing Glu Met Leu Val Pro 200 205 210 atg ttg ttg ccc ttc aaa ccc acc gca att tac tcg gcg gtg ccc gat 787 Met Leu Leu Pro Phe Lys Pro Thr Wing He Tyr Ser Wing Val Pro Asp 215 220 225 cgc tgc caa gcc acc gcg ctc ccc ctt gcc gat gag 'ctc ggc ctc gac 835 Arg Cys Gln Ala Thr Ala Leu Pro Leu Ala Asp Glu Leu Gly Leu Asp 230 235 240 '245 gtg tcc gtc aac cga ctg gtc gac gac gcc tgg gaa ccc acc 883 Val Ser Val Asn Arg Leu Phe Gly Asp Asp Wing Trp Glu Thr Asp Pro 250 255". 260 gag gcc tgc aag aag cgc ttc acc gac gtg gtc gcg caa ggt ggc gtg 931 Glu Wing Cys Lys Lys Arg Phe Thr Asp Val Val Wing Gln Gly Gly Val 265 270 275 ccg atg atc gtt ggg cag ggc gac atc att ccg gaa atg atc aaa tgg 979 Pro Met He Val Gly Gln Gly Asp He He Pro Glu Met He Lys Trp 280 285 290 ttc tcc gag aac ggc acc ctc ect atc gat gag aag aac gcg aaa 1027 Phe Ser Glu Asn Gly Thr Leu Pro He Asp Glu Lys He Lys Wing Lys 295 300 305 aag ggc age gtg tgg gtg ttg age ttt cac gac ggt gtg gtc ttc acc ggc 1075 Lys Gly Ser Val Trp Val Leu Ser Phe His Asp Gly Val Phe Thr Gly 310 315 320 325 gct gat tac ctg gcg agt tcc ctg ccg gtt a aa taggagcgcg tttaaggcct 1128 Wing Asp Tyr Leu Wing Ser Ser Leu Pro Val Lys 330 335 cca 1131 < 210 > 102 < 211 > 336 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 102 Met Wing Asn Lys Asn Asn Lys Pro His Glu Val Asp Lys Asp Gln Asp 1 5 10 15 Being Wing Met Leu He Asn Gly Arg Leu Gln Gln He Pro Wing Arg Pro 20 25 30 Thr Glu Glu Phe Thr Arg Pro Thr Leu Wing Ala Gly Ala Val Leu Trp 35 40 45 Arg Gly Asp He Thr Asn Pro Asp Ser He Glu Val Ala Val He His 50 55 60 Arg Pro His Tyr Asp Asp Trp Ser Leu Ala Lys Gly Lys Val Asp Pro 65 70 75 80 Gly Glu Be He Pro Thr Thr Wing Wing Arg Glu He Leu Glu Glu Thr 85 90 95 Gly Tyr Asp He Arg Leu Gly Lys Leu He Gly Lys Val Thr Tyr Pro 100 105 110 Val Leu Asp Arg Thr Lys Val Val Tyr Tyr Trp Thr Ala Gln Val Leu 115 120 125 Gly Gly Glu Phe Val Pro Asn Asp Glu Val Asp Glu He Arg Trp Leu 130 135 140 Ser Val Asp Glu Ala Cys Glu Leu Leu Ser Tyr Gln Val Asp Thr Glu 145 150 155 160 Val Leu Ala Lys Ala Ala Lys Arg Phe Arg Thr Pro Ser Thr Thr Arg 165 170 175 Val Leu Tyr Val Arg His Wing His Wing His Gly Arg Gln Thr Trp Gly 180 185 190 Gly Asp Asp Asn Lys Arg Pro Leu Asp Lys Lys Gly Arg Arg Gln Wing 195 200 205 Glu Met Leu Val Pro Met Leu Leu Pro Phe Lys Pro Thr Wing He Tyr 210 215 220 Ser Wing Val Pro Asp Arg Cys Gln Wing Thr Wing Leu Pro Leu Wing Asp 225 230 235 240 Glu Leu Gly Leu Asp Val Ser Val Asn Arg Leu Phe Gly Asp Asp Wing 245 250 255 Trp Glu Thr Asp Pro Glu Wing Cys Lys Lys Arg Phe Thr Asp Val Val 260 265 270 Wing Gln Gly Gly Val Pro Met He Val Gly Gln Gly Asp He He Pro 275 280 285 Glu Met He Lys Trp Phe Ser Glu Asn Gly Thr Leu Pro He Asp Glu 290 295 300 Lys He Lys Wing Lys Lys Gly Ser Val Trp Val Leu Ser Phe His Asp 305 310 315 320 Gly Val Phe Thr Gly Wing Asp Tyr Leu Wing Ser Ser Leu Pro Val Lys 325 330 335 < 210 > 103 < 211 > 651 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (628) < 223 > RXS01528 < 400 > 103 cacccaaacc caaacctctc agtcgaataa gcagaagtct caggacaacc gcaggggtaa 60 gggtcgtagg tctccaacca ggaggcgttc caacacgagg gtg aat cag gcg tgg 115 Val Asn Gln Wing Trp 1 5 cag cag tcc cgt ttg gtt act tct gat gag act tcc gca ggt ggt ctc 163 Gln Gln Ser Arg Leu Val Thr Ser Asp Glu Thr Ser Wing Gly Gly Leu 10 15 20 gtg gtg gtt gtt tea gtg gcg gcg gtc aac gct aac aat gag gtt gat 211 Val Val Ser Gly Leu Wing Glu Wing Val Asn Wing Asn Asn Glu Val Asp 25 30 35 ctg tcg aag att tat gtt gcg ttg att ggt cgc ctt gat cgt cgt ggt 259 Leu Ser Lys He Tyr Val Ala Leu He Gly Arg Leu Asp Arg Arg Gly 40 45 50 cgt ttg ttg tgg tcg atg ccg aag ggc cat gtt gag ect ggt gag gat 307 Arg Leu Leu Trp Ser Met Pro Lys Gly His Val Glu Pro Gly 'Glu Asp 55 60 65 aag gct gcg act gct gag cgt gag gtg tgg gag gag acc ggc atc cac 355 Lys Wing Wing Thr Wing Glu Arg Glu Val Trp Glu Glu Thr Gly He His 70 75 80 85 ggt gag gtg ttc act gag ttg ggt gtg att gat tat tgg ttc gtt tcg 403 Gly Glu Val Phe Thr Glu Leu Gly Val He Asp Tyr Trp Phe Val Ser 90 95 100 gaa ggg aag cgg atc cat aag acg gtg cat cat cat ttg ttg cgt tat 451 Glu Gly Lys Arg He His Lys Thr Val His His His Leu Leu Arg Tyr 105 110 115 gtt gat ggc gat ttg aat gat gag gat cca gaa gtc act gag gtg gcg 499 Go l Asp Gly Asp Leu Asn Asp Glu Asp Pro Glu Val Thr Glu Val Wing 120 125 130 tgg att ccg gcg aat cag ttg att gag cat ttg gct ttt gcg gat gag 547 Trp He Pro Wing Asn Gln Leu He Glu His Leu Wing Phe Wing Asp Glu 135 140 145 cgg aag ttg gct agg cag gcg cat gat ttg ect gag ttt gct ttg 595 Arg Lys Leu Ala Arg Gln Ala His Asp Leu Leu Pro Glu Phe Ala Leu 150 155 160 165 aag gaa aag gcg gag gga agg tcc acc cca agg tgattccgaa ccccaacccg 648 Lys Glu Lys Wing Glu Gly Arg Ser Thr Pro Arg 170 175 aac 651 < 210 > 104 < 211 > 176 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 104 Val Asn Gln Wing Trp Gln Gln Ser Arg Leu Val Thr Ser Asp Glu Thr 1 5 10 15 Be Wing Gly Gly Leu Val Val Ser Gly Leu Wing Glu Wing Val Asn Wing 20 25 30 Asn Asn Glu Val Asp Leu Ser Lys He Tyr Val Ala Leu He Gly Arg 35 40 45 ^ 4 Leu Asp Arg Arg Gly Arg Leu Leu Trp Ser Met Pro Lys Gly His Val 50 55 60 Glu Pro Gly Glu Asp Lys Ala Wing Thr Wing Glu Arg Glu Val Trp Glu 65 70 75 80 Glu Thr Gly He His Gly Glu Val Phe Thr Glu Leu Gly Val He Asp 85 90 95 Tyr Trp Phe Val Ser Glu Gly Lys Arg He His Lys Thr Val His His 100 105 110 His Leu Leu Arg Tyr Val Asp Gly Asp Leu Asn Asp Glu Asp Pro Glu 115 120 125 Val Thr Glu Val Wing Trp He Pro Wing Asn Gln Leu He Glu His Leu 130 135 140 Wing Phe Wing Asp Glu Arg Lys Leu Wing Arg Gln Wing His Asp Leu Leu 145 150 155 160 Pro Glu Phe Ala Leu Lys Glu Lys Ala Glu Gly Arg Ser Thr Pro Arg 165 170 175 < 210 > 105 < 211 > 509 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (486) < 223 > RXS01716 < 400 > 105 gaa gtc act ect gag gga ttc aaa gag atc acc cgt gaa aac acc atc 48 Glu Val Thr Pro Glu Gly Phe Lys Glu He Thr Arg Glu Asn Thr He 1 5 10 15 gtt cgc ctg ggc aaa ggc gtc gac gcc acc ggt cag cta gac ccc gag 96 Val Arg Leu Gly Lys Gly Val Asp Wing Thr Gly Gln Leu Asp Pro Glu 20 25 30 gca atc gag cgc act cgt gtc gct ttg gaa aac tac gtt gaa ctc atg 144 Wing He Glu Arg Thr Arg Val Wing Leu Glu Asn Tyr Val Glu Leu Met 35 40 45 gaa acc cat ggg gta gag gcc gta gga cg atg gtt gcc acc tcc gca acc 192 Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr 50 55 60 cgc gat gcg tcc aac cgc gat gaa ttc ttt tcg atg acc cgc cag ctt 240 Arg Asp Wing Being Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu 65 70 75 80 ctg tcc aa g atc cgt ect gga tac caa gct gaa gta att tcc ggc gaa 288 Leu Ser Lys He Arg Pro Gly Tyr Gln Wing Glu Val He Ser Gly Glu 85 90 95 gag gaa gct ctg ctg tcc ttc cga ggt gca atc gtt gac ctg ect gaa 336 Glu Glu Ala Leu Leu Ser Phe Arg Gly Ala Hep Val Asp Leu Pro Glu 100 105 110 5 gac caa ggt ect ttc tgt gtt atc gac ctt ggc ggt gga tcc act gag 384 • Asp Gln Gly Pro Phe Cys Val He Asp Leu Gly Gly Gly Ser Thr Glu 115 120 125 ttc atc gtt ggc acc tac gac ggt gaa atc cta ggc tcc cac tea acc 432 Phe He Val Gly Thr Tyr Asp Gly Glu He Leu Gly Ser His Ser Thr 130 135 140 caa atg gga tgc gtg cgc ctg acc gaa cga atc atg cgc age gac cca 480 Gln Met Gly Cys Val Arg Leu Thr Glu Arg He Met Arg Ser Asp Pro 145 150 155 160 10 ccc gac tgaaaccgaa gtggaaatcg ccc 509 Pro Asp < 210 > 106 < 211 > 162 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 106 Glu Val Thr Pro Glu Gly Phe Lys Glu He Thr Arg Glu Asn Thr He 15 1 5 10 15 Val Arg Leu Gly Lys Gly Val Asp Wing Thr Gly Gln Leu Asp Pro Glu 20 25 30 Wing He Glu Arg Thr Arg Val Wing Leu Glu Asn Tyr Val Glu Leu Met 35 40 45 Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr 50 55 60 Arg Asp Ala Ser Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu 20 65 70 75 80 Leu Ser Lys He Arg Pro Gly Tyr Gln Wing Glu Val He Ser Gly Glu 85 90 95 Glu Glu Wing Leu Leu Ser Phe Arg Gly Wing He Val Asp Leu Pro Glu 100 105 110 Asp Gln Gly Pro Phe Cys Val He Asp Leu Gly Gly Gly Ser Thr Glu 115 120 '125 Phe He Val Gly Thr Tyr Asp Gly Glu He Leu Gly Ser His Ser Thr 25 130 135 140 Gln Met Gly Cys Val Arg Leu Thr Glu Arg He Met Arg Ser Asp Pro 145 150 155 160 Pro Asp < 210 > 107 < 211 > 654 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (631) < 223 > RXS01835 < 400 > 107 tcaacatcta ttcctcctgc gatttgcatg ggatatatat taaaaattct agccgaaagt 60 ttcctgcgtg aatacacttt ccccgcgcct tcgcaaagct atg aat act gcc gcg 115 Met Asn Thr Ala Ala 1 5 tgg gca cac cgc cac cac gta cgc aaa ggc ggt gga att ccg tat gtc 163 Trp Ala His Arg His His Val Arg Lys Gly Gly Gly He Pro Tyr Val 10 15 20 age cat ctt tat tea gtg atg tac ttg ctg gcc age gtc act aat gat 211 Ser His Leu Tyr Ser Val Met Tyr Leu Leu Wing Ser Val Thr Asn Asp 25 30 35 gaa gat gtg ctc atc gcc ggg ctg ctc cac gac acc ctc gaa gac gta 259 Glu Asp Val Leu He Wing Gly Leu Leu His Asp Thr Leu Glu Asp Val 40 45 50 ccc gag gaa tac aat tct gcc caa ctt gaa gct gat ttt ggt ccg cgg 307 Pro Glu Glu Tyr Asn Ser Wing Gln Leu Glu Wing Asp Phe Gly Pro Arg 55 60 65 gtg cgc gag ttg gtg gaa gag ctc acc aaa cag ccc tta aaa age tgg 355 Val Arg Glu Leu Val Glu Glu Leu Thr Lys Gln Pro Leu Lys Ser Trp 70 75 80 85 aaa gcg cgt gcc gac gct tac ctc ctg cac ctc age gca ggt gcc age 403 Lys Ala Arg Ala Asp Ala Tyr Leu Leu His Leu Be Wing Gly Wing Be 90 95 100 tta gag gct gtc tta atc tcc acc gca gat aaa ctg cat aat ctc atg 451 Leu Glu Wing Val Leu He Ser Thr Wing Asp Lys Leu His Asn Leu Met 105 110 115 tcc atc ttg gat gac ctt gaa ata cac ggt gaa gat tta tgg cag cgc 499 Ser He Leu Asp Asp Leu Glu He His Gly Glu Asp Leu Trp Gln Arg 120 125 130 ttt aac gct ggc aaa gag cag caa atc tgg tgg tat age gag gtt tat 547 Phe Asn Wing Gly Lys Glu Gln Gln He Trp Trp Tyr Ser Glu Val Tyr 135 140 145 cag ata tct ctc cag cgc tta ggg ttc aat gag ttg aat aaa caa ctg 595 Gln He Ser Leu Gln Arg Leu Gly Phe Asn Glu Leu Asn Lys Gln Leu 150 155 160 165 ggg ttg tgc gtc gaa aag ctc tta aag caa age gcc taggcgctcg 641 Gly Leu Cys Val Glu Lys Leu Leu Lys Gln Ser Ala 170 175 gcggcgtcga taa 654 < 210 > 108 < 211 > 177 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 108 Met Asn Thr Wing Wing Trp Wing His Arg His His Val Arg Lys Gly Gly 1 5 10 15 Gly He Pro Tyr Val Ser His Leu Tyr Ser Val Met Tyr Leu Leu Wing 20 25 30 Ser Val Thr Asn Asp Glu Asp Val Leu He Wing Gly Leu Leu His Asp 35 40 45 Thr Leu Glu Asp Val Pro Glu Glu Tyr Asn Ser Wing Gln Leu Glu Wing 50 55 60 Asp Phe Gly Pro Arg Val Arg Glu Leu Val Glu Glu Leu Thr Lys Gln 65 70 75 80 Pro Leu Lys Ser Trp Lys Wing Arg Wing Asp Wing Tyr Leu Leu His Leu 85 90 95 Wing Wing Gly Wing Ser Leu Glu Wing Val Leu He Ser Thr Wing Asp Lys 100 105 110 Leu His Asn Leu Met Ser He Leu Asp Asp Leu Glu He His Gly Glu 115 120 125 Asp Leu Trp Gln Arg Phe Asn Wing Gly Lys Glu Gln Gln He Trp Trp 130 135 140 Tyr Ser Glu Val Tyr Gln He Ser Leu Gln Arg Leu Gly Phe Asn Glu 145 150 155 160 Leu Asn Lys Gln Leu Gly Leu Cys Val Glu Lys Leu Leu Lys Gln Ser 165 170 175 Wing < 210 > 109 < 211 > 1050 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1027) < 223 > RXS02497 < 400 > 109 tcgatgccgc cgctggcgaa gactcgggga aacctaaaaa taccgaagaa gaatttgacc 60 gattcacact ttgccaccct agaccgtcta acctttaggt gtg aga tta ggt gta 115 Val Arg Leu Gly Val 1 5 tta gat gtg ggc age aat act gtc cac cta gtt gca gta gac gcg cgt 163 Leu Asp Val Gly Ser Asn Thr Val His Leu Val Wing Val Asp Wing Arg 10 15 20 ccc ggt gga cac ccc acc ccg atg age aat tgg cgt acc cca ctg cgc 211 Pro Gly Gly His Pro Thr Pro Met Ser Asn Trp Arg Thr Pro Leu Arg 25 30 35 ctt gtt gag ctt ctt gat gac tcc ggg gcg atc tcc gaa aag ggc atc 259 Leu Val Glu Leu Leu Asp Asp Ser Gly Wing He Ser Glu Lys Gly He 40 45 50 aac aaa ctc gtc acc gca gtc gag gca gca gca gc gac cta gcg aaa acg 307 Asn Lys Leu Thr Ser Wing Val Gly Glu Wing Wing Asp Leu Wing Lys Thr 55 60 65 ctc ggc tgc gct gaa ctg atg cca ttt gct here tcg gca gtc cgc tcc 355 Leu Gly Cys Wing Glu Leu Met Pro Phe Wing Thr Ser Wing Val Arg Ser 70 75 80 85 gcc acc aac age gag gca gtg ctc gac cac gtg gag aag gaa acc ggc 403 Wing Thr Asn Ser Glu Wing Val Leu Asp His Val Glu Lys Gl u Thr Gly 90 95 100 gtc cgc ctc tcc atc ctt tcc ggt gaa gac gaa gca cgc caa act ttc 451 Val Arg Leu Ser He Leu Ser Gly Glu Asp Glu Ala Arg Gln Thr Phe 105 110 115 ctc gca gtt cga cgt tgg tat gga tgg tcc gca ggg ggg cgc ata act aac 499 Leu Wing Val Arg Arg Trp Tyr Gly Trp Ser Wing Gly Arg He Thr Asn 120 125 130 ctc gac atc ggt ggc ggc tcc ctg gaa cta tcc tcc gga acc gac gaa 547 Leu Asp He Gly Gly Gly Ser Leu Glu Leu Ser Ser Gly Thr Asp Glu 135 140 145 tcc cca gac ctc gcg ttc tea ctg gat ctg ggc gcg ggc cgc ttg acc 595 Pro Pro Asp Leu Ala Phe Ser Leu Asp Leu Gly Ala Gly Arg Leu Thr 150 155 160 165 cac aac tgg tcc gac ccg gca ccg gc aaac aac aac aac ctc 643 His Asn Trp Phe Asp Thr Asp Pro Pro Wing Arg Lys Lys He Asn Leu 170 175 180 ctc cgc gat atc gat gcg gaa ctt gca gaa ccc gcc cgc cag atg 691 Leu Arg Asp Tyr He Asp Wing Glu Leu Wing Glu Pro Wing Arg Gln Met 185 185 195 cgc acc cta ggg ccc gcg cgc ctg gca gtg gga here tcc aaa act ttc 739 Arg Thr Leu Gly Pro Wing Arg Leu Wing Val Gl and Thr Ser Lys Thr Phe 200 205 210 cgc acc ctg gca cga cg ggt gct gcg ccc tea tcc gca gga cca 787 Arg Thr Leu Wing Arg Leu Thr Gly Ala Wing Pro Being Ser Wing Gly Pro 215 220 225 cac gtc acc acga acc ctc acc gcg ccg ggt ctg cgc cag ctg atc gca 835 His Val Thr Arg Thr Leu Thr Wing Pro Gly Leu Arg Gln Leu He Wing 230 235 240 245 ttt atc tea cga atg act gcg gcg gac cgc gct gag ctg gaa ggt atc 883 phe He Be Arg Met Thr Wing Wing Asp Arg Wing Glu Leu Glu Gly He 250 255 260 age tcg gat cgg tea cat cag atc gtg gca ggt gcg cta gtt gcg gaa 931 Ser Ser Asp Arg Ser His Gln He Val Ala Gly Ala Leu Val Ala Glu 265 270 275 gct gcg atg cgt gcg ttg gat att gac aag gta gaa att tgt ccg tgg 979 Ala Ala Met Arg Ala Leu Asp He Asp Lys Val Glu He Cys Pro Trp 280 285 290 gca ctt cgt gaa ggt gtg atc ctc acc agg atc gac aaa gga ctc gag 1027 Wing Leu Arg Glu Gly Val He Leu Thr Arg He Asp Lys Gly Leu Glu 295 300 305 taacatttac ccggaaagga gtt '1050 < 210 > 110 < 211 > 309 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 110 Val Arg Leu Gly Val Leu Asp Val Gly Ser Asn Thr Val His Leu Val 1 5 10 15 Ala Val Asp Ala Arg Pro Gly Gly His Pro Thr Pro Met Ser Asn Trp 20 25 30 Arg Thr Pro Leu Arg Leu Val Glu Leu Leu Asp Asp Ser Gly Wing He 35 40 45 Ser Glu Lys Gly He Asn Lys Leu Thr Ser Wing Val Gly Glu Wing Wing 50 55 60 Asp Leu Wing Lys Thr Leu Gly Cys Wing Glu Leu Met Pro Phe Wing Thr 65 70 75 80 Ser Ala Val Arg Ser Ala Thr Asn Ser Glu Ala Val Leu Asp His Val 85 90 95 Glu Lys Glu Thr Gly Val Arg Leu Ser He Leu Ser Gly Glu Asp Glu 100 105 110 Wing Arg Gln Thr Phe Leu Wing Val Arg Arg Trp Tyr Gly Trp Wing 115 115 125 Gly Arg He Thr Asn Leu Asp He Gly Gly Gly Leu Glu Leu Ser 130 135 140 Ser Gly Thr Asp Glu Ser Pro Asp Leu Ala Phe Ser Leu Asp Leu Gly 145 150 155 160 Wing Gly Arg Leu Thr His Asn Trp Phe Asp Thr Asp Pro Pro Wing Arg 165 170 175 Lys Lys He Asn Leu Leu Arg Asp Tyr He Asp Wing Glu Leu Wing Glu 180 185 190 Pro Wing Arg Gln Met Arg Thr Leu Gly Pro Wing Arg Leu Wing Val Gly 195 200 205 Thr Ser Lys Thr Phe Arg Thr Leu Wing Arg Leu Thr Gly Ala Ala Pro 210 215 220 Ser Ser Ala Gly Pro His Val Thr Arg Thr Leu Thr Ala Pro Gly Leu 225 230 235 240 Arg Gln Leu He Wing Phe He Being Arg Met Thr Wing Wing Asp Arg Wing 245 250 255 Glu Leu Glu Gly Gly Be Ser Asp Arg Ser His Gln He Val Wing Gly 260 265 270 Wing Leu Val Wing Glu Wing Wing Met Arg Wing Leu Asp He Asp Lys Val 275 280 285 Glu He Cys Pro Trp Wing Leu Arg Glu Gly Val He Leu Thr Arg He 290 295 300 Asp Lys Gly Leu Glu 305 < 210 > 111 < 211 > 534 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (511) < 223 > RXS02972 < 400 > 111 acctacgacg gtgaaatcct aggctcccac tcacccaaa tgggatgcgt gcgcctgacc 60 gaacgaatca tgcgcagcga cccacccgac tgaaaccgaa gtg gaa atc gcc cgc 115 Val Glu He Ala Arg 1 5 gac tac gtt gca gaa cgc atc cag gaa gta aaa gcc atc gtc cca att 163 Asp Tyr Val Ala Glu Arg He Gln Glu Val Lys Ala He Val Pro Pro 10 15 20 tea aag gca aaa acc ttt gtg gga tgc gca ggc acc ttc acc atc atc 211 Ser Lys Wing Lys Thr Phe Val Gly Cys Wing Gly Thr Phe Thr Thr He 25 30 35 tcc gcc tgg gtg caa ggc cta gaa age tac gac cgc gac gcg atc cac 259 Ser Wing Trp Val Gln Gly Leu Glu Ser Tyr Asp Arg Asp Ala He His 40 45 50 ctc tct gca ctc aac ttc gat gca ctg cga gtt gtc acc gat gag atc 307 Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val Val Thr Asp Glu He 55 60 65 att tea gaa tea tea tea cag cgc gcc age aac cca gtt gtt gat cca 355 He Ser Glu Ser Ser Ser Gln Arg Ala Ser Asn Pro Val Val Asp Pro 70 75 80 85 ggt cgc gcc gac gtc atc ggt ggc gga gtcc ct gtt gtt gtc caa gca gcg 403 Gly Arg Wing Asp Val He Gly Gly Gly Ser Val Val Val Gln Ala Wing 90 95 100 atc gac tta gcc tcc aaa gaa gcc ggt gta gac tac atc att att tcc 451 He Asp Leu Wing Ser Lys Glu Wing Gly Val Asp Tyr He He He Ser 105 110 115 gaa aaa gac atc ctc gac ggc ctc atc ctt ggc ctg gta gaa gcc gac 499 Glu Lys Asp He Leu Asp Gly Leu He Leu Gly Leu Val Glu Wing Asp 120 125 130 tct tg aag aaa taggacccta gttttaaacc act 534 Ser Leu Lys Lys 135 < 210 > 112 < 211 > 137 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 112 Val Glu He Wing Arg Asp Tyr Val Wing Glu Arg He Gln Glu Val Lys 1 5 10 15 Wing He Val Pro He Ser Lys Wing Lys Thr Phe Val Gly Cys Wing Gly 20 25 30 Thr Phe Thr Thr He Ser Wing Trp Val Gln Gly Leu Glu Being Tyr Asp 35 40 45 Arg Asp Wing He His Leu Being Wing Leu Asn Phe Asp Wing Leu Arg Val 50 55 60 Val Thr Asp Glu He He Ser Glu Ser Ser Being Gln Arg Wing Ser Asn • 65 70 75 80 Pro Val Val Asp Pro Gly Arg Ala Asp Val He Gly Gly Gly Ser Val 85 90 95 Val Val Gln Ala Ala He Asp Leu Ala Ser Lys Glu Ala Gly Val Asp 100 105 110 Tyr He He He Ser Glu Lys Asp He Leu Asp Gly Leu He Leu Gly 115 120 125 10 Leu Val Glu Ala Asp Ser Leu Lys Lys 130 135 < 210 > 113 < 211 > 636 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS <; 222 > (101) .. (613) 15 < 223 > RXA02159 < 400 > 113 tgatggacca gcgtccaaag ttttcgatga agcagaaaac cgcctccacg ctcagaaagc 60 actgctggtg tggctgctgg ccaaccagcc gaggtaagac atg ccc ggc tea 115 Met Ser Leu Gly Ser 1 5 acc ccg tea here ccg gaa aac tta aat ccc gtg act cgc act gca cgc 163 Thr Pro Ser Thr Pro Glu Asn Leu Asn Pro Val Thr Arg Thr Wing Arg 10 15 20 20 caa gct ctc att ttg cag att ttg gac aaa caa aaa gtc acc age cag 211 Gln Ala Leu He Leu Gln He Leu Asp Lys Gln Lys Val Thr Ser Gln 25 30 35 gta caa ctg tct gaa ttg ctg gat gaa ggc atc gat atc acc cag 259 Val Gln Leu Ser Glu Leu Leu Asu Glu Gly He Asp He Thr Gln 40 45 50 gcc acc ttg tcc cga gat ctc gat gaa ctc ggt gca cgc aag gtt cgc 307 Wing Thr Leu Ser Arg Asp Leu Asp Glu Leu Gly Wing Arg Lys Val Arg 55 60 65 25 ccc gat ggg gga cgc gcc tac tac gcg gtc gcc cca gta gat age atc 355 Pro Asp Gly Gly Arg Wing Tyr Tyr Wing Val Gly Pro Val Asp Being He 70 75 80 85 gcc cgc gaa gat ctc cgg ggt ccg tcg gag aag ctg cgc cgc atg ctt 403 Wing Arg Glu Asp Leu Arg Gly Pro Ser Glu Lys Leu Arg Arg Met Leu 90 95 100 gat gaa ctg ctg gtt tct here gat cat tcc ggc aac atc gcg atg ctg 451 Asp Glu Leu Leu Val Ser Thr Asp His Ser Gly Asn He Wing Met Leu 105 110 115 cgc acc ccg gc gct gcc cag tac ctg gca agt ttc atc gat agg 499 Arg Thr Pro Pro Gly Wing Wing Gln Tyr Leu Wing Being Phe He Asp Arg 120 125 130 gtg ggg ctg aaa gaa gtc gtt ggc acc atc gct ggt gat gac acc gtt 547 Val Gly Leu Lys Glu Val Val Gly Thr He Ala Gly Asp Asp Thr Val 135 140 145 ttc gtt ctc gcc cgt gat ccg ctc here ggt aaa gaa cta ggt gaa tta 595 Phe Val Leu Ala Arg Asp Pro Leu Thr Gly Lys Glu Leu Gly Glu Leu 150 155 160 165 ctc age ggg cgc acc act taaagcgccc ctagttcaag gct 636 Leu Ser Gly Arg Thr Thr 170 < 210 > 114 < 211 > 171 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 114 Met Ser Leu Gly Ser Thr Pro Ser Thr Pro Glu Asn Leu Asn Pro Val 1 5 10 15 Thr Arg Thr Wing Arg Gln Wing Leu He Leu Gln He Leu Asp Lys Gln 20 25 30 Lys Val Thr Ser Gln Val Gln Leu Ser Glu Leu Leu Asu Glu Gly 35 40 45 He Asp He Thr Gln Wing Thr Leu Ser Arg Asp Leu Asp Glu Leu Gly 50 55 60 Wing Arg Lys Val Arg Pro Asp Gly Gly Arg Wing Tyr Tyr Wing Val Gly 65 70 75 80 Pro Val Asp Be He Wing Arg Glu Asp Leu Arg Gly Pro Ser Glu Lys 85 90 95 Leu Arg Arg Met Leu Asp Glu Leu Leu Val Ser Thr Asp His Ser Gly 100 105 110 Asn He Wing Met Leu Arg Thr Pro Pro Gly Ala Wing Gln Tyr Leu Ala 115 120 125 Be Phe He Asp Arg Val Gly Leu Lys Glu Val Val Gly Thr He Ala 130 135 140 Gly Asp Asp Thr Val Phe Val Leu Ala Arg Asp Pro Leu Thr Gly Lys 145 150 155 160 Glu Leu Gly Glu Leu Leu Ser Gly Arg Thr Thr • 165 170 < 210 > 115 < 211 > 486 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (463) 10 < 223 > RXA02201 < 400 > 115 tctaccagcc aaatcatcaa ctcatagcga aggaatcaac ttcatgaata atcaaccatc 60 • agtacttttc gtttgcgtcg gcaatggtgg aaaatctcaa atg gcc gca gcg cta 115 Met Ala Ala Ala Leu 1 5 gcc aaa aaa cat gcc ggg gac gct ctc aaa gtt tat tea gct ggc here 163 Ala Lys Lys His Ala Gly Asp Ala Leu Lys Val Tyr Ser Ala Gly Thr 10 15 20 15 aag cca ggt acg aaa tta aat caa tc cct gat tct att gct gaa 211 Lys Pro Gly Thr Lys Leu Asn Gln Gln Ser Leu Asp Ser He Wing Glu 25 30 35 gtt ggc gca gat atg tct caa ggg ttt cca aag ggc att gac cag gag 259 Val Gly Wing Asp Met Ser Gln Gly Phe Pro Lys Gly He Asp Gln Glu 40 45 50 • tta att aag cga gta gac cgc gtg gtc att ctt ggt gcc gaa gct caa 307 Leu He Lys Arg Val Asp Arg Val Val He Leu Gly Ala Glu Ala Gln 55 60 65 20 cta gaa atg ect atc gat gca aac ggc ata cta cag cgc tgg gta act 355 Leu Glu Met Pro He Asp Ala Asn Gly He Leu Gln Arg Trp Val Thr 70 75 80 85 gac gaa ccc tct gaa cgt gga att gaa ggt atg gaa cgc atg cgc ctg 403 Asp Glu Pro Ser Glu Arg Gly He Glu Gly Met Glu Arg Met Arg Leu 90 95 100 gtc cga gat gat att gac g cc cga gtc caa aac ctc gtc gct gaa cta 451 Val Arg Asp Asp He Asp Ala Arg Val Gln Asn Leu Val Ala Glu Leu 105 110 115 25 acc aac gca tagcagtttt ctaatctcac here 486 Thr Gln Asn Wing 120 < 210 > 116 < 211 > 121 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 116 Met Ala Ala Ala Ala Ala Lys Lys His Ala Gly Asp Ala Leu Lys Val 1 5 10 15 Tyr Ser Ala Gly Thr Lys Pro Gly Thr Lys Leu Asn Gln Gln Ser Leu 20 25 30 Asp Ser He Ala Ala Glu Val Gly Ala Asp Met Being Gln Gly Phe Pro Lys 35 40 45 Gly He Asp Gln Glu Leu He Lys Arg Val Asp Arg Val Val He Leu 50 55 60 Gly Wing Glu Wing Gln Leu Glu Met Pro He Asp Wing Asn Gly He Leu 65 70 75 80 Gln Arg Trp Val Thr Asp Glu Pro Ser Glu Arg Gly He Glu Gly Met 85 90 95 Glu Arg Met Arg Leu Val Arg Asp Asp Asp Ala Arg Val Gln Asn 100 105 110 Leu Val Ala Glu Leu Thr Gln Asn Ala 115 120 < 210 > 117 < 211 > 510 < 212 > DNA <; 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (487) < 223 > RXA00599 < 400 > 117 gaacgatcgg ccctttgatt gaagtcccag tattagtcgg attggtttat gtcatgttgt 60 ggcttggacc aaaaatcttt aaaaaggaga atgcaggatc atg aaa tea gtt ttg 115 Met Lys Ser Val Leu 1 5 ttt gtg tgc gtc ggt aat ggc gga aaa tea cag atg gcg gcg gcg ctg 163 Phe Val Cys Val Gly Asn Gly Gly Lys Ser Gln Met Wing Ala Wing Leu 10 15 20 gca cag aag tat gca tea gat tea gta gag atc cat gt tct gct gga acc 211 Wing Gln Lys Tyr Wing Ser Asp Ser Val Glu He His Wing Wing Gly Thr 25 30 35 aag ect gca cag ggg cta aac caa ttg tct gtg gaa tcc atc gct gag 259 Lys Pro Wing Gln Gly Leu Asn Gln Leu Ser Val Glu Ser He Wing Glu 40 45 50 gtg ggc gct gat atg tcg caa gga att ccc aaa gcg atc gat ccg gag 307 Val Gly Wing Asp Met Ser Gln Gly He Pro Lys Wing Asp Pro Glu 55 60 65 ctg cgc act gtc gat cgt gtg gtt att ttg ggc gat gac gca cag 355 Leu Leu Arg Thr Val Asp Arg Val Val He Leu Gly Asp Asp Ala Gln 70 75 80 85 gta gat atg ect gaa tct gca cag ggc gct ctt gag cgt tgg tea att 403 Val Asp Met Pro Glu Be Wing Gln Gly Wing Leu Glu Arg Trp Be He 90 95 100 gag gac ccg gat gct caa ggt atg gaa cgt atg cgt att gtg cgg gat 451 Glu Glu Pro Asp Wing Gln Gly Met Glu Arg Met Arg He Val Arg Asp 105 110 115 cag atc gat aac cga gtc caa gct ttg cta gcg gga taagcgccga 497 Gln He Asp Asn Arg Val Gln Wing Leu Leu Wing Gly 120 125 aaaaggggca tgt 510 < 210 > 118 < 211 > 129 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 118 Met Lys Ser Val Leu Phe Val Cys Val Gly Asn Gly Gly Lys Ser Gln 1 5 10 15 Met Ala Ala Ala Ala Gln Lys Tyr Ala Ser Asp Ser Val Glu He 20 25 30 His Ser Ala Gly Thr Lys Pro Ala Gln Gly Leu Asn Gln Leu Ser Val 35 40 45 Glu Ser He Wing Glu Val Gly Wing Asp Met Ser Gln Gly He Pro Lys 50 55 60 Wing He Asp Pro Glu Leu Leu Arg Thr Val Asp Arg Val Val He Leu 65 70 75 80 Gly Asp Asp Wing Gln Val Asp Met Pro Glu Be Wing Gln Gly Wing Leu 85 90 95 Glu Arg Trp Ser He Glu Glu Pro Asp Wing Gln Gly Met Glu Arg Met 100 105 110 Arg He Val Arg Asp Gln He Asp Asn Arg Val Gln Ala Leu Leu Ala 115 120 125 Gly < 210 > 119 < 211 > 1221 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1198) < 223 > RXA00600 < 400 > 119 cggagttaat gagcggtagg tggatgggtg cggtcatgtc cgtcattata tattgacgca 60 catcgatatt gaaggtattt ttatatcggc aaacatcaat atg att gaa ggc tgg 115 Met He Glu Gly Trp 1 5 ctc atg acc ctt act aaa gag cat tcg here ect cga gcg gct ggc tea 163 Leu Met Thr Leu Thr Lys Glu His Be Thr Pro Arg Wing Wing Gly Be 10 15 20 atg tcg ttt ctt gac cgc tgg tta gct gcc tgg att ttc ttg gct atg 211 Met Ser Phe Leu Asp Arg Trp Leu Wing Wing Trp He Phe Leu Wing Met 25 30 35 gct ggg ggg ttg tta atc ggc aag gtc ttt cca gga att ggg gcg ctt 259 Wing Wing Gly Leu Leu He Gly Lys Val Phe Pro Gly He Gly Wing Leu 40 45 50 ttg age gcg gtg gaa att ggt gga att tcc att cca att gct atc ggt 307 Leu Ser Wing Val Glu He Gly Gly He Ser He Pro He Wing He Gly 55 60 65 ttg atc gtc atg tat cca ect ttg gcc aag gtg cgc tac gac aaa 355 Leu He Val Met Met Tyr Pro Pro Leu Ala Lys Val Arg Tyr Asp Lys 70 75 80 85 act aaa gaa atc age here gac cgc gct ctc atg gtg gtg tcg att atg 403 Thr Lys Glu He Ser Thr Asp Arg Ala Leu Met Val Val Ser He Met 90 95 100 ttg aac tgg atc gtt gga cca gca ctt atg ttt age ctg gcg tgg ctg 451 Leu Asn Trp He Val Gly Pro Wing Leu Met Phe Ser Leu Wing Trp Leu 105 110 115 ttt ctt cca cca gat cca gac ctt cgc act ggg cta att atc gtg ggc 499 Phe Leu Pro Asp Gln Pro Glu Leu Arg Thr Gly Leu He He Val Gly 120 125 130 ctt gcg cgc tgt atc gcg atg gtt ttg gta tgg agt gat ctc gct tgt 547 Leu Ala Arg Cys He Ala Met Val Leu Val Trp Ser Asp Leu Wing Cys 135 140 145 ggt gac cgg gaa gca act gct gtg ctg gtt gca atc aac tcg gtg ttc 595 Gly Asp Arg Glu Ala Thr Ala Val Leu Val Ala He Asn Ser Val Phe 150 155 160 165 5 cag atc ctt atg ttc ggt gtg ctt ggt tgg ttt tac ctg cag att ctt 643 • Gln He Leu Met Phe Gly Val Leu Gly Trp Phe Tyr Leu Gln He Leu 170 175 180 ccc tcg tgg ctg gga tta gac acc acg tcg gtg act ttc tct gtg gta 691 Pro Ser Trp Leu Gly Leu Asp Thr Thr Ser Val Thr Phe Ser Val Val 185 190 195 tea atc gtg act tcc gtt ctc gtg ttc ttg ggc atacca ctt gta gct 739 Ser He Val Thr Ser Val Leu Val Phe Leu Gly He Pro Leu Val Wing 200 205 210 10 99a gtt tta tct cgc gtc att ggt gaa aaa here aag gga cgg cgc tgg 787 Gly Val Leu Ser Arg Val He Gly Glu Lys Thr Lys Gly Arg Arg Trp 215 220 225 • tac gag gac acg ttc ctg ect aag att tea ccc tcg gcg ctg att ggc 835 Tyr Glu Asp Thr Phe Leu Pro Lys He Ser Pro Leu Ala Leu He Gly 230 235 240 245 ttg cta tac here att gtt ctg ctg ttt tcg tg gg gat gaa atc 883 Leu Leu Tyr Thr He Val Leu Leu Phe Ser Leu Gln Gly Asp Glu He 250 255 260 15 here gcg cag ect tgg here gta gct cgt ctt gca ttg ccg ctg ctg atg 931 Thr Ala Gln Pro Trp Thr Val Wing Arg Leu Wing Leu Pro Leu Leu Met 265 270 275 tac ttt gtg ggc atg ttt ttc att tcc ctg gtg gta tcc aaa ctg tcc 979 Tyr Phe Val Gly Met Phe Phe He Ser Leu Val Val Ser Lys Leu Ser 280 285 290 • ggg tta act tat gag cga gct gct tc gtg tct act gca gca gga gga 1027 Gly Leu Thr Tyr Glu Arg Ala Wing Ser Val Ser Phe Thr Ala Wing Gly 295 300 305 20 aac aac ttt gaa tta gcg att gcg gta tcg atc gga acc ttt ggt gcg 1075 Asn Asn Phe Glu Leu Wing He Wing Val Ser He Gly Thr Phe Gly Wing 310 315 320 325 here tea ccg cag gta tta gct gga acg atc ggc ect ttg att gaa gtc 1123 Thr Ser P Ro Gln Ala Leu Wing Gly Thr He Gly Pro Leu He Glu Val 330 335 340 cca gta tta gtc gga ttg gtt tat gtc atg ttg tgg ctt gga cca aaa 1171 Pro Val Leu Val Gly Leu Val Tyr Val Met Leu Trp Leu Gly Pro Lys 345 350 355 25 ate ttt aaa aag gag aat gca gga tea tgaaatcagt tttgtttgtg 1218 ^ ^ ^ GÉÉfe He Phe Lys Lys Glu Asn Wing Gly Ser 360 365 tgc 1221 < 210 > 120 < 211 > 366 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 120 Met He Glu Gly Trp Leu Met Thr Leu Thr Lys Glu His Ser Thr Pro 1 5 10 15 Arg Wing Wing Gly Ser Met Ser Phe Leu Asp Arg Trp Leu Wing Wing Trp 20 25 30 He Phe Leu Wing Ala Wing Wing Gly Leu Leu He Gly Lys Val Phe Pro 35 40 45 Gly He Gly Wing Leu Leu Ser Wing Val Glu He Gly Gly He Ser He 50 55 60 Pro He Wing He Gly Leu He Val Met Met Tyr Pro Pro Leu Ala Lys 65 70 75 80 Val Arg Tyr Asp Lys Thr Lys Glu He Ser Thr Asp Arg Ala Leu Met 85 90 95 Val Val Ser He Met Leu Asn Trp He Val Gly Pro Ala Leu Met Phe 100 105 110 Ser Leu Ala Trp Leu Phe Leu Pro Asp Gln Pro Glu Leu Arg Thr Gly 115 120 125 Leu He He Val Val Gly Leu Ala Arg Cys He Ala Met Val Leu Val Trp 130 135 140 Ser Asp Leu Ala Cys Gly Asp Arg Glu Ala Thr Ala Val Leu Val Ala 145 150 155 160 He Asn Ser Val Phe Gln He Leu Met Phe Gly Val Leu Gly Trp Phe 165 170 175 Tyr Leu Gln He Leu Pro Ser Trp Leu Gly Leu Asp Thr Thr Ser Val 180 185 190 Thr Phe Ser Val Val Ser He Val Thr Ser Val Leu Val Phe Leu Gly 195 200 205 He Pro Leu Va l Wing Gly Val Leu Ser Arg Val He Gly Glu Lys Thr 210 215 220 Lys Gly Arg Arg Trp Tyr Glu Asp Thr Phe Leu Pro Lys He Ser Pro 225 230 235 240 Leu Ala Leu He Gly Leu Leu Tyr Thr He Val Leu Leu Phe Ser Leu 245 250 255 Gln Gly Asp Glu He Thr Wing Gln Pro Trp Thr Val Wing Arg Leu Wing 260 265 270 Leu Pro Leu Leu Met Tyr Phe Val Gly Met Phe Phe He Ser Leu Val 275 280 285 Val Ser Lys Leu Ser Gly Leu Thr Tyr Glu Arg Wing Wing Ser Val Ser 290 295 300 Phe Thr Wing Wing Gly Asn Asn Phe Glu Leu Wing He Wing Val Ser He 305 310 315 320 Gly Thr Phe Gly Wing Thr Ser Pro Gln Wing Leu Wing Gly Thr He Gly 325 330 335 Pro Leu He Glu Val Pro Val Leu Val Gly Leu Val Tyr Val Met Leu 340 345 350 Trp Leu Gly Pro Lys He Phe Lys Lys Glu Asn Wing Gly Ser 355 360 365 < 210 > 121 < 211 > 1233 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1210) < 223 > RXA02200 < 400 > 121 attgtgtgga gagtggtcat aaatccacta tatattgacg aatgtcgata ttgaaagtat 60 tttgaatatc gacaggtatc aattacgc aaaggtgtcgc atg aac aac tea act 115 Met Thr Asn Ser Thr 1 5 cag acg cgg gcc aag cca gcc cga atc tea ttt ctt gat aaa tac att 163 Gln Thr Arg Ala Lys Pro Ala Arg He is Phe Leu Asp Lys Tyr He 10 15 20 cca ctt tgg att att tg gcg atg gcg ttt ggg cta ttt tta ggc cgg 211 Pro Leu Trp He He Leu Ala Met Wing Phe Gly Leu Phe Leu Gly Arg 25 30 35 age gtt tcg gga ctc tea ggc ttt cta ggc gca atg gaa gtc gga ggg 259 Ser Val Ser Gly Leu Ser Gly Phe Leu Gly Wing Met Glu Val Gly Gly 40 45 50 atc tcc tcc cca atc gct tta gcc ctc ctt ct gta atg atc tac cc ccg 307 He Be Leu Pro He Wing Leu Gly Leu Leu Val Met Met Tyr Pro Pro 55 60 65 ttg gcc aaa gtt cgg tat gac aaa act aaa caa att gcc act gat aag 355 Leu Wing Lys Val Arg Tyr Asp Lys Thr Lys Gln He Wing Thr Asp Lys 70 75 80 85 cat ttg atg ggc gtg tea ctc att ctc aat tgg gtg gtg gctt ect gcc 403 His Leu Met Gly Val Ser Leu He Leu Asn Trp Val Val Gly Pro Wing 90 95 100 tta atg ttc gcg cta gct tgg ttg ttc ctc cca gac caa ccg 'gaa tta 451 Leu Met Phe Wing Leu Wing Trp Leu Phe Leu Pro Asp Gln Pro Glu Leu 105 110 115 cga acc ggc ctg att att gta gga ctc gca cga tgt att gcg atg gtc 499 Arg Thr Gly Leu He He Val Gly Leu Ala Arg Cys He Wing Met Val 120 125 130 ttg gtt tgg tct gat atg tcc tgt gga gac cgc gag gct here gca gtt 547 Leu Val Trp Ser Asp Met Ser Cys Gly Asp Arg Glu Wing Thr Wing Val 135 140 145 ctc gta gcc att aat tea gtt ttt caa gtc gca atg ttt ggt gca ctt 595 Leu Val Ala He Asn Ser Val Phe Gln Val Ala Met Phe Gly Ala Leu 150 155 160 165 ggc tgg ttc tat ctg caa gtt tta cca tcc tgg cta gga tta cca act 643 Gly Trp Phe Tyr Leu Gln Val Leu Pro Ser Trp Leu Gly Leu Pro Thr 170 175 180 action acc gct caa ttc tct ttc tgg tea att gtg act tcg gtt ttg gtg 691 Thr Thr Wing Gln Phe Ser Phe Trp Ser He Val Thr Ser Val Leu Val 185 190 195 ttc ctc gga ata ect cta ctt gct gga gtt ttc tcg cga att att ggc 739 Phe Leu Gly He Pro Leu Leu Wing Gly Val Phe Ser Arg He He Gly 200 205 210 gaa aag atc aag gga cgt gag tgg tat gaa caa aag ttc ctt ccg gca 787 Glu Lys He Lys Gly Arg Glu Trp Tyr Glu Gln Lys Phe Leu Pro Wing 215 220 225 atc tct cca ttt gca cta atc ggt ctg ctt tat acg atc gtc ttg ttg 835 He Ser Pro Phe Ala Leu He Gly Leu Leu Tyr Thr He Val Leu Leu 230 235 240 245 ttt tea ttg caa ggt gat cag atc gtc tct caa cca tgg gct gta gtt 883 Phe Ser Leu Gln Gly Asp Gln He Val Ser Gln Pro Trp Wing Val Val 250 255 260 cgt ctc gcg atacca ttg gtt atc tat ttc gtt gga atg ttt ttc att 931 Arg Leu Wing Pro Pro Leu Val He Tyr Phe Val Gly Met Phe Phe He 265 270 275 tea ctc att gcg tea aaa cta tct ggc atg aac tat gca aag tct gca 979 Ser Leu He Ala Ser Lys Leu Ser Gly Met Asn Tyr Wing Lys Ser Wing 280 285 290 tcc gtc tct tct tcct act gcc gcc aac aat ttt gaa ctt gcg att gcg 1027 Ser Val Ser Phe Thr Wing Wing Gly Asn Asn Phe Glu Leu Wing Wing 295 300 305 gtg tcg a tc gga acg ttt ggc gca act tct gca cag gct atg gca gga 1075 Val Ser He Gly Thr Phe Gly Ala Thr Ser Ala Gln Ala Met Ala Gly 310 315 320 325 5 acg att ggt ccc ttg att gaa att cca gta ctt gtc ggc ttg gtc tac 1123 Thr He Gly Pro Leu He Glu He Pro Val Leu Val Gly Leu Val Tyr 330 335 340 gcc atg ctg tgg cta ggc ccc aag ttg ttc cca aat gac ccc acg ctg 1171 Wing Met Leu Trp Leu Gly Pro Lys Leu Phe Pro Asn Asp Pro Thr Leu 345 350 355 cca tea tea gct ccttctctct caa atc atc aac tea tagcgaagga 1220 Pro Being Being Wing Arg Ser Thr Being Gln He He Asn Ser 360 365 370 10 atcaaettea tga 1233 < 210 > 122 < 211 > 370 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 122 Met Thr Asn Ser Thr Gln Thr Arg Wing Lys Pro Wing Arg He Ser Phe 1 5 10 15 15 Leu Asp Lys Tyr He Pro Leu Trp He He Leu Wing Met Wing Phe Gly 20 25 30 Leu Phe Leu Gly Arg Ser Val Ser Gly Leu Ser Gly Phe Leu Gly Wing 35 40 45 Met Glu Val Gly Gly He Ser Leu Pro He Wing Leu Gly Leu Leu Val 50 55 60 Met Met Tyr Pro Pro Leu Ala Lys Val Arg Tyr Asp Lys Thr Lys Gln 65 70 75 80 20 He Wing Thr Asp Lys His Leu Met Gly Val Ser Leu He Leu Asn Trp 85 90 95 Val Val Gly Pro Ala Leu Met Phe Ala Leu Ala Trp Leu Phe Leu Pro 100 105 110 Asp Gln Pro Glu Leu Arg Thr Gly Leu He He Val Gly Leu Ala Arg 115 120 125 Cys He Ala Met Val Leu Val Trp Ser Asp Met Ser Cys Gly Asp Arg 130 135 140 25 Glu Ala Thr Ala Val Leu Val Ala He Asn Ser Val Phe Gln Val Ala 145 150 155 160 Met Phe Gly Ala Leu Gly Trp Phe Tyr Leu Gln Val Leu Pro Ser Trp 165 170 175 Leu Gly Leu Pro Thr Thr Wing Gln Phe Ser Phe Trp Ser He Val 180 185 190 Thr Ser Val Leu Val Phe Leu Gly He Pro Leu Leu Wing Gly Val Phe 195 200 205 Ser Arg He He Gly Glu Lys He Lys Gly Arg Glu Trp Tyr Glu Gln 210 215 220 Lys Phe Leu Pro Wing He Ser Pro Phe Ala Leu He Gly Leu Leu Tyr 225 230 235 240 Thr He Val Leu Leu Phe Ser Leu Gln Gly Asp Gln He Val Ser Gln 245 250 255 Pro Trp Wing Val Val Arg Leu Wing Pro Pro Leu Val He Tyr Phe Val 260 265 270 Gly Met Phe Phe He Ser Leu He Wing Ser Lys Leu Ser Gly Met Asn 275 280 285 Tyr Ala Lys Ser Ala Ser Val Phe Thr Wing Wing Gly Asn Asn Phe 290 295 300 Glu Leu Wing He Wing Val Ser He Gly Thr Phe Gly Wing Thr Ser Wing 305 310 315 320 Gln Wing Ala Wing Gly Thr He Gly Pro Leu He Glu He Pro Val Leu 325 330 335 Val Gly Leu Val Tyr Ala Met Leu Trp Leu Gly Pro Lys Leu Phe Pro 340 345 350 Asn Asp Pro Thr Leu Pro Ser Ser Ala Arg Ser Thr Ser Gln He He 355 360 365 Asn Ser 370 < 210 > 123 < 211 > 762 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (739) < 223 > RXA02202 < 400 > 123 cgctgaacta acccaaaacg catagcagtt ttctaatctc acacatcttc aacaccgtta 60 aatctattgg tttccccgta aaatcttcga aaggaagaac atg acc ggg caa gct 115 Met Thr Gly Gln Ala 1 5 gca cca aac ttg cat acc aat att ttg aac cgt atc gca aat gaa ctg 163 Ala Pro Asn Leu His Thr Asn He Leu Asn Arg He Wing Asn Glu Leu 10 15 20 gcg ttg acc tat ca gga gtt ttc tct gc gag act atc aac cgc tat 211 Wing Leu Thr Tyr Gln Gly Val Phe Ser Wing Glu Thr He Asn Arg Tyr 25 30 35 att ttt gaa tcg tat gtg tcg ttg gcg aga here gca aaa atc cat acg 259 He Phe Glu Ser Tyr Val Ser Leu Ala Arg Thr Ala Lys He His Thr 40 45 50 cac ctg cca att ttg gca gaa ggt ttt gct aaa gac cgg ctg cac gca 307 His Leu Pro He Leu Wing Glu Gly Phe Wing Lys Asp Arg Leu His Wing 55 60 65 ctt gcg gta gct gaa ggt aag gtg gct tea ect gtg ect cag gtc cta 355 Leu Ala Val Ala Glu Gly Lys Val Ala Ser Val Pro Pro Gln Val Leu 70 75 80 85 ttt att tgc gtc cac aac gca ggt cgt tea caa att gct tcg gcg ttg 403 Phe He Cys Val His Asn Wing Gly Arg Ser Gln He Wing Being Wing Leu 90 95 100 ttg tct cac tat gcc ggt agt tct gta gag gta cgt tct gca ggt tct 451 Leu Ser His Tyr Wing Gly Ser Val Val Glu Val Arg Ser Wing Gly Ser 105 110 115 tta ect gct tct gaa att cac cca ctg gtg ttg gaa att ttg tea gag 499 Leu Pro Wing Ser Glu He His Pro Leu Val Leu Glu He Leu Ser Glu 120 125 130 cga gga gtg aac att tct gat gca ttt ccg aaa ccg cta acc gat gat 547 Arg Gly Val Asn He Ser Asp Wing Phe Pro Lys Pro Leu Thr Asp Asp 135 140 145 gtt att cgc gca tct gac tat gtc ata here atg gga tgt gga gat gtg 595 Val He Arg Wing As Asp Tyr Val He Thr Met Gly Cys Gly Asp Val 150 155 160 165 tgc cca atg tat cca gga aag cac tat ctc gat tgg gag ctc gct gat 643 Cys Pro Met Tyr Pro Gly Lys His Tyr Leu Asp Trp Glu Leu Wing Asp 170 175 180 ccg tea gat gaa ggt gag gac aag ata cag gaa ata att gag gaa att 691 Pro Ser Asp Glu Gly Glu Asp Lys He Gln Glu He He Glu Glu He 185 190 195 gac ggt cga atc cgc gag ctt tgg aaa age att caa tta tcg caa aac 739 Asp Gly Arg He Arg Glu Leu Trp Lys Ser He Gln Leu Ser Gln Asn 200 205 210 taggcagtca aaggtctggc acc 762 < 210 > 124 < 211 > 213 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 124 Met Thr Gly Gln Wing Wing Pro Asn Leu His Thr Asn He Leu Asn Arg 1 5 10 15 He Wing Asn Glu Leu Wing Leu Thr Tyr Gln Gly Val Phe Wing Wing Glu 20 25 30 Thr He Asn Arg Tyr He Phe Glu Ser Tyr Val Ser Leu Ala Arg Thr 35 40 45 Wing Lys He His Thr His Leu Pro He Leu Wing Glu Gly Phe Wing Lys 50 55 60 Asp Arg Leu His Wing Leu Wing Val Wing Glu Gly Lys Val Wing Pro Pro 65 70 75 80 Val Pro Gln Val Leu Phe He Cys Val His Asn Wing Gly Arg Ser Gln 85 90 95 Wing Wing Wing Wing Leu Leu Ser His Tyr Wing Gly Ser Val Val Val 100 105 110 Arg Wing Wing Ser Leu Pro Wing Ser Glu He His Pro Leu Val Leu 115 120 125 Glu He Leu Ser Glu Arg Gly Val Asn He Ser Asp Wing Phe Pro Lys 130 135 140 Pro Leu Thr Asp Asp Val He Arg Wing Ser Asp Tyr Val He Thr Met 145 150 155 160 Gly Cys Gly Asp Val Cys Pro Met Tyr Pro Gly Lys His Tyr Leu Asp 165 170 175 Trp Glu Leu Wing Asp Pro Be Asp Glu Gly Glu Asp Lys He Gln Glu 180 185 190 He He Glu Glu He Asp Gly Arg He Arg Glu Leu Trp Lys Ser He 195 200 205 Gln Leu Ser Gln Asn 210 < 210 > 125 < 211 > 1002 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (979) < 223 > RXA02205 < 400 > 125 gcccaaaccg agcctggata cccgcaaacc acttgaaccg accattcgct gtttcacgcc 60 caccacacta ctgaggtcat aaggtagtac ggtagatcgg gtg aat gaa gag ata 115 Val Asn Glu Glu He 1 5 acc ctc cta gcc gca gca gat ect gcc gca act gaa aat att ggc 163 Thr Leu Leu Ala Ala Ala Ala Asp Pro Ala Ala Thr Glu Asn He Gly 10 15 20 tgg gta caa acc att gtg ctc tcc atc gtt caa ggc ctc here gag ttc 211 Trp Val Gln Thr He Val Leu Ser He Val Gln Gly Leu Thr Glu Phe 25 30 35 ctg ccg atc age tcc age gga cac ctc cga atc atc tct gag ctg ttc 259 Leu Pro He Ser Ser Gly His Leu Arg He He Ser Glu Leu Phe 40 45 50 tgg ggt gcc gat gcc ggc gcg tcc ttt acc gcc gtg gtt cag ctt ggt 307 Trp Gly Ala Asp Ala Gly Ala Ser Phe Thr Ala Val Val Gln Leu Gly 55 60 65 acc gaa gcc gca gtg ctg gtg ttt ttt gcc aag gaa atc tgg caa atc 355 Thr Glu Ala Ala Val Leu Val Phe Phe Ala Lys Glu He Trp Gln He 70 75 80 85 atc here ggt tgg ttc gct ggc gta ttc aat aag gaa cgc cgc gga ttt 403 He Thr Gly Trp Phe Wing Gly Val Phe Asn Lys Glu Arg Arg Gly Phe 90 95 100 gaa tac cgc atg ggc tgg atg atc att gtt gcc acc att ccc gtc gtg 451 Glu Tyr Arg Met Gly Trp Met He He Val Wing Thr He Pro Val Val 105 110 115 atc ttg ggt gtg ttg ggc aag gac ctg atc cgt gag gcg ctg cga aat 499 He Leu Gly Val Leu Gly Lys Asp Leu He Arg Glu Ala Leu Arg Asn 120 125 130 atg tgg atc act gca aka gtg ctg atc ctg ttc aka ctg gtg ttc att 547 Met Trp He Thr Ala Ser Val Leu He Leu Phe Ser Leu Val Phe He 135 140 145 ttg gcc gag aag atg ggc aag aag gaa cgc gac tac gac aaa ctg acc 595 Leu Wing Glu Lys Met Gly Lys Lys Glu Arg Asp Tyr Asp Lys Leu Thr 150 155 160 165 atg aaa gat gcc atc atc atg ggt ctt gca cag tgt ctt gcg ctg atc 643 Met Lys Asp Ala He He Met Gly Leu Ala Gln Cys Leu Ala Leu He 170 175 180 ect ggc gtg tct cgc aka ggc ggc acc atc tct gct ggt ttg ttc ctt 691 Pro Gly Val Ser Arg Ser Gly Gly Thr He Ser Ala Gly Leu Phe Leu 185 190 195 ggt ctc aag cgt gta gaa gcc acc aag ttc aka ttc ctg ctg gca atc 739 Gly Leu Lys Arg Glu Val Ala Thr Lys Phe Ser Phe Leu Leu Ala He 200 205 210 ect gca gtg ctt ggc aka ggt ttg tac aka ctg ect gac gct ttt gcg 787 Pro Ala Val Leu Gly Ser Gly Leu Tyr Ser Leu Pro Asp Ala Phe Ala 215 220 225 cca age aka gga caa gct gcc aka ggc cta cag ctc acc gtg ggt acc 835 Pro Ser Ser Gly Gln Ala Ala Ser Gly Leu Gln Leu Thr Val Gly Thr 230 235 240 245 ctg gtt gcc ttc gta gtt ggc tac att aka att gcg tgg ctg atg aag 883 Leu Val Ala Phe Val Val Gly Tyr He Ser He Wing Trp Leu Met Lys 250 255 260 ttc gtg gca aac cac tcc ttc age tgg ttt gct gca tac cgt att ect 931 Phe Val Wing Asn His Ser Phe Ser Trp Phe Wing Wing Tyr Arg He Pro 265 270 275 gca ggt ctg ctc gtg atg ctg ctg ctc gca ctg ggc atg ctc aac cca 979 Wing Gly Leu Leu Val Met Leu Leu Leu Wing Leu Gly Met Leu Asn Pro 280 285 290 taaaattcct gtacatetta aaa 1002 < 210 > 126 < 211 > 293 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 126 Val Asn Glu Glu He Thr Leu Leu Wing Wing Wing Asp Pro Wing Wing 1 5 10 15 Thr Glu Asn He Gly Trp Val Gln Thr He Val Leu Ser He Val Gln 20 25 30 Gly Leu Thr Glu Phe Leu Pro He Ser Ser Ser Gly His Leu Arg He 35 40 45 He Ser Glu Leu Phe Trp Gly Wing Asp Wing Gly Wing Being Phe Thr Wing 50 55 60 Val Val Gln Leu Gly Thr Glu Wing Wing Val Leu Val Phe Phe Wing Lys 65 70 75 80 Glu He Trp Gln He He Thr Gly Trp Phe Wing Gly Val Phe Asn Lys 85 90 95 Glu Arg Arg Gly Phe Glu Tyr Arg Met Gly Trp Met He He Val Val Ala 105 105 110 Thr He Pro Val Val He Leu Gly Val Leu Gly Lys Asp Leu He Arg 115 120 125 Glu Ala Leu Arg Asn Met Trp He Thr Ala Ser Val Leu He Leu Phe 130 135 140 Ser Leu Val Phe He Leu Ala Glu Lys Met Gly Lys Lys Glu Arg Asp 145 150 155 160 Tyr Asp Lys Leu Thr Met Lys Asp Wing He Met Met Gly Leu Wing Gln 165 170 175 Cys Leu Ala Leu He Pro Gly Val Ser Arg Ser Gly Thr He Ser 180 185 190 Wing Gly Leu Phe Leu Gly Leu Lys Arg Glu Val Wing Thr Lys Phe Ser 195 200 205 Phe Leu Leu Wing He Pro Wing Val Leu Gly Ser Gly Leu Tyr Ser Leu 210 215 220 Pro Asp Wing Phe Wing Pro Being Ser Gly Gln Wing Wing Ser Gly Leu Gln 225 230 235 240 Leu Thr Val Gly Thr Leu Val Wing Phe Val Val Gly Tyr He Ser He 245 250 255 Wing Trp Leu Met Lys Phe Val Wing Asn His Ser Phe Ser Trp Phe Wing 260 265 270 Wing Tyr Arg He Pro Wing Gly Leu Leu Val Met Leu Leu Leu Ala Leu 275 280 285 Gly Met Leu Asn Pro 290 < 210 > 127 < 211 > 975 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (952) < 223 > RXA00900 < 400 > 127 gtgcggtggc tgcgctggtc gctagtgtgg tgtgcgcgct ggcgccgtcg ataagcgtat 60 tagtgatcgc acgcctggtg caggggcttg gcggcggtgc gtg cgt ggt att gcg 115 Val Arg Gly He Wing 1 5 cgc gcg atc gtg cca gac ctt gaa cgc gga caa aag gct gcg cac gcc 163 Arg Ala He Val Pro Asp Leu Glu Arg Gly Gln Lys Wing Wing His Wing 10 15 20 ttt gca ctg ctg atg att att cag gga att gct ccc gtg gta gct ccg 211 Phe Ala Leu Leu Met He He Gln Gly He Ala Pro Val Val Ala Pro 25 30 35 ctc att ggt ggt gtg gtg ctg gtc ggg ect ttt ggc tgg cgg gga att ttc 259 Leu He Gly Gly Val Leu Val Gly Pro Phe Gly Trp Arg Gly He Phe 40 45 50 tgg gca ctt gca ctg gtg aat ttt gcg cag ctg ctt gtt gct ttg ctg 307 Trp Ala Leu Ala Leu Val Asn Phe Ala Gln Leu Leu Val Ala Leu Leu 55 60 65 cag att aag gag tcg aag cca gtt gaa gag cgt acc gca gca gga ctt 355 Gln He Lys Glu Ser Lys Pro Val Glu Glu Arg Thr Ala Wing Gly Leu 70 75 80 85 ggc gga atg ctg tcc aac tat gtc ttt gtg ctg aat ect cact ttt 403 Gly Gly Met Leu Ser Asn Tyr Val Phe Val Leu Lys Asn Pro Gln Phe 90 95 100 ttg gca tat gta ttc here ttg ggg ctg tct ttt ggg gcg atg ttc tcc 451 Leu Ala Tyr Val Phe Thr Leu Gly Leu Ser Phe Gly Wing Met Phe Ser 105 110 115 tac att tcg gcg tcg ccg ttc gtg ctg cag aat caa atg ggc att ccg 499 Tyr He Ser Ala Ser Pro Phe Val Leu Gln Asn Gln Met Gly He Pro 120 125 130 gta ctg ctg tat tcc att att ttc gga gtg aat gct ttt ggt ttg att 547 Val Leu Leu Tyr Ser He He Phe Gly Val Asn Ala Phe Gly Leu He 135 140 145 gtg ggc gga atg gtc aat agg cga ctt ctg cag cgg att cat cca cac 595 Val Gly Gly Met Val Asn Arg Arg Leu Leu Gln Arg He His Pro His 150 155 160 165 cgc atc atg caa act gtg ctg gcc agt ttt act gtg ctg tgt gcg ctt 643 Arg He Met Gln Thr Val Leu Wing Ser Phe Thr Val Leu Cys Wing Leu 170 175 180 ttg ctg att gaa gtg ctg ttt att aat tgg ata ccg ctg ttc ctg ttg 691 Leu Leu He Glu Val Leu Phe He Asn Trp He Pro Leu Phe Leu Leu 185 190 195 ctg ctg ttt ctt atc gtt tcc cat att ccg atg gtt atg gct aac gcg 739 Leu Leu Phe Leu He Val Ser His He Pro Met Val Met Wing Wing Asn Wing 200 205 210 here gct ctg gga act gaa gtg gtg cga age agg gcg gga tcg ggt tct 787 Thr Ala Leu Gly Thr Glu Val Val Arg Ser Arg Ala Gly Ser Gly Ser 215 220 225 gca att ttg ggt ttc gtg caa ttc acg atg ggt gct ttg gtg agt tea 835 Ala He Leu Gly Phe Val Gln Phe Thr Met Gly Ala Leu Val Ser Ser 230 235 240 245 ctg gtc gga tta ggc tct gat aag gct ttg act atg gga atc gca atg 883 Leu Val Gly Leu Gly Ser Asp Lys Ala Leu Thr Met Gly He Ala Met 250 255 260 act gct tgt gca ctg ctg gcg tgt ggg tgt gcg tac ctg gca ggt cga 931 Thr Wing Cys Wing Leu Leu Wing Cys Gly Cys Wing Tyr Leu Wing Gly Arg 265 270 275 aaa ggt att cca gaa atg aag tagctctagg tggcgtttta agg 975 Lys Gly He Pro Glu Met Lys 280 < 210 > 128 < 211 > 284 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 128 Val Arg Gly He Wing Arg Wing He Val Pro Asp Leu Glu Arg Gly Gln 1 5 10 15 Lys Ala Wing His Wing Phe Wing Leu Leu Met He He Gln Gly He Wing 20 25 30 Pro Val Val Wing Pro Leu He Gly Gly Val Leu Val Gly Pro Phe Gly 35 40 45 Trp Arg Gly He Phe Trp Ala Leu Ala Leu Val Asn Phe Ala Gln Leu 50 55 60 Leu Val Ala Leu Leu Gln He Lys Glu Ser Lys Pro Val Glu Arlu 65 70 75 80 Thr Wing Wing Gly Leu Gly Gly Met Leu Ser Asn Tyr Val Phe Val Leu 85 90 95 Lys Asn Pro Gln Phe Leu Wing Tyr Val Phe Thr Leu Gly Leu Ser Phe 100 105 110 Gly Wing Met Phe Ser Tyr He Ser Wing Ser Pro Phe Val Leu Gln Asn 115 120 125 Gln Met Gly He Pro Val Leu Leu Tyr Ser He He Phe Gly Val Asn 130 135 140 Wing Phe Gly Leu He Val Gly Gly Met Val Asn Arg Arg Leu Leu Gln 145 150 155 160 Arg He His Pro His Arg He Met Gln Thr Val Leu Wing Ser Phe Thr 165 170 175 Val Leu Cys Ala Leu Leu Leu He Glu Val Leu Phe He Asn Trp He 180 185 190 Pro Leu Phe Leu Leu Leu Leu Phe Leu He Val His He Pro Met Met 195 200 205 Val Met Ala Asn Ala Thr Ala Leu Gly Thr Glu Val Val Arg Ser Arg 210 215 220 Wing Gly Ser Gly Ser Wing He Leu Gly Phe Val Gln Phe Thr Met Gly 225 230 235 240 Ala Leu Val Ser Ser Leu Val Gly Leu Gly Ser Asp Lys Ala Leu Thr 245 250 255 Met Gly He Ala Met Thr Ala Cys Ala Leu Leu Ala Cys Gly Cys Ala 260 265 270 Tyr Leu Ala Gly Arg Lys Gly He Pro Glu Met Lys 275 280 < 210 > 129 < 211 > 537 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (514) < 223 > RXN00901 < 400 > 129 ttttaagtat tggtgctatc ttccggtgct gatggtacct gaatgaaaat ttetaattaa 60 aaataccccc aaatettega tatagataca cgagacagtg atg cag aaa aaa caa 115 Met Gln Lys Lys Gln 1 5 cag ctg age acc gcc ctg att atg gga ttg gca tta ttg tea gcc age 163 Gln Leu Ser Thr Ala Leu He Met Gly Leu Ala Leu Leu Ser Ala Be 10 15 20 tcc gcg cta gcg act gat atg tat ttg ccg gca atg ect ggt att gcg 211 Be Ala Leu Ala Thr Asp Met Tyr Leu Pro Ala Met Pro Gly He Ala 25 30 35 gaa gat ttg ggg here act gca ccg atg gtg cag tta act ctt tct tcc 259 Glu Asp Leu Gly Thr Thr Wing Pro Met Val Gln Leu Thr Leu Ser Ser 40 45 50 ttt atg gct gg atg gcg att ggc cag ttg atc att ggt ect ttg tcg 307 Phe Met Wing Gly Met Wing He Gly Gln Leu He He Gly Pro Leu Ser 55 60 65 gat caa ttg gga agg aaa ggc ctg ctc gtt gca ggt gcg gtg gct gcg 355 Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Wing Gly Ala Val Wing Ala 70 75 80 85 ctg gtc gtc gtg gtg gtg gcg ctg gcg gcg tcg ata age gta tta 403 Leu Val Ala Ser Val Val Cys Ala Leu Ala Pro Ser He Ser Val Leu 90 95 100 gtg atc gca cgc ctg gtg cag ggg ctt ggc ggc ggt gcg tgc gtg gta 451 Val He Wing Arg Leu Val Gln Gly Leu Gly Gly Gly Wing Cys Val Val 105 110 115 ttg cgc gcg cga tcg tgc cag acc ttg aac gcg gac aaa agg ctg cgc 499 Leu Arg Ala Arg Ser Cys Gln Thr Leu Asn Wing Asp Lys Arg Leu Arg 120 125 130 acg ect ttg cac tgc tgatgattat tcagggaatt gct 537 Thr Pro Leu His Cys 135 < 210 > 130 < 211 > 138 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 130 Met Gln Lys Lys Gln Gln Leu Ser Thr Wing Leu He Met Gly Leu Wing 1 5 10 15 Leu Leu Ser Wing Being Wing Leu Wing Thr Asp Met Tyr Leu Pro Wing 20 25 30 Met Pro Gly He Wing Glu Asp Leu Gly Thr Thr Wing Pro Met Val Gln 35 40 45 Leu Thr Leu Be Ser Phe Met Wing Gly Met Wing He Gly Gln Leu He 50 55 60 He Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Wing 65 70 75 80 Gly Ala Val Ala Ala Leu Val Ala Ser Val Val Cys Ala Leu Ala Pro 85 90 95 Ser He Ser Val Leu Val He Ala Arg Leu Val Gln Gly Leu Gly Gly 100 105 110 Gly Ala Cys Val Val Leu Arg Ala Arg Ser Cys Gln Thr Leu Asn Wing 115 120 125 Asp Lys Arg Leu Arg Thr Pro Leu His Cys 130 135 < 210 > 131 < 211 > 501 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (478) < 223 > FRXA00901 < 400 >; 131 aectgaatga aaatttetaa ttaaaaatac ccccaaatct tcgatataga tacaegagac 60 agtgatgcag aaaaaacaac agctgagcac cgccctgatt atg gga ttg gca tta 115 Met Gly Leu Ala Leu 1 5 ttg tea gcc age tcc gcg cta gcg act gat atg tat ttg ccg gca atg 163 Leu Ser Ala Ser Ser Ala Leu Ala Thr Asp Met Tyr Leu Pro Wing Met 10 15 20 ect ggt att gcg gaa gat ttg ggg here act gca ccg atg gtg cag tta 211 Pro Gly He Wing Glu Asp Leu Gly Thr Thr Wing Pro Met Val Gln Leu 25 30 35 act ctt tct tcc ttt atg gct gga atg gcg att ggc caa ttg atc att 259 Thr Leu Ser Being Phe Met Wing Gly Met Wing He Gly Gln Leu He He 40 45 50 ggt ect ttg tcg gat caa ttg gga agg aaa ggc ctg ctc gtt gca ggt 307 Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly Leu Leu Val Wing Gly 55 60 65 gcg gtg gcg gcg gtc ctg gtc gtg gtg gtg gcg gcg gcg ccg gcc 355 Wing Val Wing Wing Leu Val Wing Val Val Cys Wing Ala Leu Wing Pro Ser 70 75 80 85 ata age gta tta gtg atc gca cgc ctg gtg cag ggg ctt ggc ggc ggt 4'03 He Ser Val Leu Val He Ala Arg Leu Val Gln Gly Leu Gly G and Gly 90 95 100 gcg tgc gtg gta ttg cgc gcg cga tcg tgc cag acc ttg aac gcg gac 451 Wing Cys Val Val Leu Arg Wing Arg Ser Cys Gln Thr Leu Asn Wing Asp 105 110 115 aaa agg ctg cgc acg ect ttg cac tgc tgatgattat tcagggaatt 498 Lys Arg Leu Arg Thr Pro Leu His Cys 120 125 gct 501 < 210 > 132 < 211 > 126 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 132 Met Gly Leu Ala Leu Leu Be Ala Be Be Ala Leu Ala Thr Asp Met 1 5 10 15 Tyr Leu Pro Wing Met Pro Gly He Wing Glu Asp Leu Gly Thr Thr Wing 20 25 30 Pro Met Val Gln Leu Thr Leu Ser Ser Phe Met Ala Gly Met Ala He 35 40 45 Gly Gln Leu He He Gly Pro Leu Ser Asp Gln Leu Gly Arg Lys Gly 50 55 60 Leu Leu Val Ala Gly Ala Val Ala Ala Leu Val Ala Ser Val Val Cys 65 70 75 80 Ala Leu Ala Pro Ser He Ser Val Val Leu Val He Ala Arg Leu Val Gln 85 90 95 Gly Leu Gly Gly Gly Ala Cys Val Val Leu Arg Ala Arg Ser Cys Gln 100 105 110 Thr Leu Asn Wing Asp Lys Arg Leu Arg Thr Pro Leu His Cys 115 120 125 < 210 > 133 < 211 > 1299 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1276) < 223 > RXA00289 < 400 > 133 cctccccata agttcactca agcaagttct cccgaacaga ttcacccgag aagtcgacag 60 accccattaa acagcccgat tcaagaaagg cttcgcagcc atg age acc acc action 115 Met Ser Thr Thr Thr 1 5 gcg ccc gaa gca cgg ttt ect gtc gtc ect ttg acc gcc atg agt ttc 163 Ala Pro Glu Ala Arg Phe Pro Val Val Pro Leu Thr Wing Met Ser Phe 10 15 20 gcg gt gt tat gtt tat gtc acg ttc gag atg ttt gt gtt gcc ctc atc 211 Wing Ala Phe Val Tyr Val Thr Phe Glu Met Phe Wing Val Gly Leu He 25 30 35 aag ccg atg gcc age gat ctt gga gtg tea gaa tcc age atc ggc ctg 259 Lys Pro Met Ala Ser Asp Leu Gly Val Ser Glu Ser Ser He Gly Leu 40 45 50 ttg atg act gtg tat gcg act gtc gtt gcc gtg gtg acg atc ect gcc 307 Leu Met Thr Val Tyr Wing Thr Val Val Wing Val Val Thr He Pro Wing 55 60 65 atg ttg tgg gtt tct cga ttt aac aag cgc here gtt ttc ctg att act 355 Met Leu Trp Val Ser Arg Phe Asn Lys Arg Thr Val Phe Leu He Thr 70 75 80 85 ctg gca ttt ttg gcc acg ggc att gtt gtt cag gca ctg acc gtt aat 403 Leu Ala Phe Leu Ala Thr Gly He Val Val Gln Ala Leu Thr Val Asn 90 95 100 tat gga atg cta gcc atc ggc cgc act atc gca gca ttg act cag ggg 451 Tyr Gly Met Leu Wing He Gly Arg Thr He Wing Wing Leu Thr His Gly 105 110 115 gtg ttt tgg gca ctt gtt ggg cca atg gca gcg cgt atg tcc cca ggt 499 Val Phe Trp Wing Leu Val Gly Pro Met Wing Wing Arg Met Ser Pro Gly 120 125 130 cac act ggt cgt gca gta ggc gtt gtg tcg att gga tea acc atg gcg 547 His Thr Gly Arg Wing Val Gly Val Val Ser He Gly Ser Thr Met Wing 135 140 145 ctg gtc gtt ggt tct ccg ctg gca here tgg atc ggt gaa ctc atc gga 595 Leu Val Val Gly Ser Pro Leu Wing Thr Trp He Gly Glu Leu He Gly 150 155 160 165 tgg cgt ect gcc acc tgg att ctt ggt gcg ctg acc att gcg gcc gtg 643 Trp Arg Pro Wing Thr Trp He Leu Gly Wing Leu Thr He Wing Wing Val 170 175 180 gct gta ctc att cca acc gtt cca tea ctg cca cca cc cca gac acg 691 Wing Val Leu He Pro Thr Val Pro Ser Leu Pro Pro Leu Pro A sp Th 185 190 195 gaa tea gag tcc aaa gaa aag aaa tcc ctt cca tgg ggt ctc att tcc 739 Glu Ser Glu Ser Lys Glu Lys Lys Ser Leu Pro Trp Gly Leu He Ser 200 205 210 ctg gtc att ttc ctt ctc ctt gcc gtc acc ggt gtt ttt gct gcc gcc tac 787 Leu Val He Phe Leu Leu Leu Wing Val Thr Gly Val Phe Wing Wing Tyr 215 220 225 ctc ctt gtc atc atc gct gaa gca ggg gac age ttc gtg 835 Thr Tyr Leu Gly Leu He He Wing Glu Thr Wing Gly Asp Ser Phe Val 230 235 240 245 tcc att ggc ttg ttc gcc ttc ggt gca ctc gga ctc att ggc gtg here 883 Ser He Gly Leu Phe Wing Phe Gly Wing Leu Gly Leu He Gly Val Thr 250 255 260 gtg gca acc cga act gtg gat caa cgc atg ctg cgt gga agt gtt cac 931 Val Wing Thr Arg Thr Val Asp Gln Arg Met Leu Arg Gly Ser Val His 265 270 275 acc ac tt gtc gtc gtc gt att att ctc gga cag atc gca ttc 979 Thr Thr Leu Phe Val Wing Wing Wing He Leu Gly Gln He Wing Phe 280 285 290 gga tta gag ggc here cta gcc gta gta gct atc ttc ctt gca gtc acc 1027 Gly Leu Glu Gly Thr Leu Wing Val Val Wing He Ph e Leu Wing Val Thr 295 300 305 gtg ttt ggt gga gca tac ggc gct ctc cca acc ctg gga acc acc atc 1075 Val Phe Gly Gly Wing Tyr Gly Wing Leu Pro Thr Leu Gly Thr Thr He 310 315 320 325 ttc ctc cat gcg ggt cgc gac cac cca gat act gca tcc tcc att tat 1123 Phe Leu His Wing Gly Arg Asp His Pro Asp Thr Wing Ser Ser He Tyr 330 335 340 gtg gtc gtc act gtg ggt atc gcg tct ggc gcg gca ctt ggc gcg 1171 Val Val Thr Tyr Gln Val Gly He Ala Ser Gly Ala Ala Leu Gly Wing 345 350 355 atg gct gtg gat gcc gat tgg gtt gct ggc act ttg tgt atc atg gct 1219 Met Wing Val Asp Wing Asp Trp Val Wing Gly Thr Leu Trp He Met Wing 360 365 370 gga ctg tea ttg gcc tcc acg ttg gcc ttg gcg ctg tgg tcc cgc ccg 1267 Gly Leu Ser Leu Wing Being Thr Leu Wing Leu Wing Leu Trp Being Arg Pro 375 380 385 cta ctg aag tagcagccca aattcagccc act 1299 Leu Leu Lys 390 < 210 > 134 < 211 > 392 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 134 Met Ser Thr Thr Thr Ala Pro Glu Ala Arg Phe Pro Val Val Pro Leu 1 5 10 15 Thr Ala Met Ser Phe Ala Ala Phe Val Tyr Val Thr Phe Glu Met Phe 20 25 30 Wing Val Gly Leu He Lys Pro Met Ala Ser Asp Leu Gly Val Ser Glu 35 40 45 Be Ser Gly Leu Leu Met Thr Val Tyr Ala Thr Val Val Ala Val 50 55 60 Val Thr He Pro Ala Met Leu Trp Val Ser Arg Phe Asn Lys Arg Thr 65 70 75 80 Val Phe Leu He Thr Leu Wing Phe Leu Wing Thr Gly He Val Val Gln 85 90 95 Wing Leu Thr Val Asn Tyr Gly Met Leu Wing He Gly Arg Thr He Wing 100 105 110 Wing Leu Thr His Gly Val Phe Trp Wing Leu Val Gly Pro Met Ala Ala 115 120 125 Arg Met Ser Pro Gly His Thr Gly Arg Ala Val Gly Val Val Ser He 130 135 140 Gly Ser Thr Met Ala Leu Val Val Gly Ser Pro Leu Ala Thr Trp He 145 150 155 160 Gly Glu Leu He Gly Trp Arg Pro Wing Thr Trp He Leu Gly Wing Leu 165 170 175 Thr He Wing Wing Val Wing Val Leu Pro Pro Thr Val Pro Ser Leu Pro 180 185 190 Pro Leu Pro Asp Thr Glu Ser Glu Ser Lys Glu Lys Lys Ser Leu Pro 195 200 205 Trp Gly Leu He Ser Leu Val He Phe Leu Leu Leu Wing Val Thr Gly 210 215 220 Val Phe Wing Wing Tyr Thr Tyr Leu Gly Leu He He Wing Glu Thr Wing 225 230 235 240 Gly Asp Ser Phe Val Ser He Gly Leu Phe Wing Phe Gly Wing Leu Gly 245 250 255 Leu He Gly Val Thr Val Wing Thr Arg Thr Val Asp Gln Arg Met Leu 260 265 270 Arg Gly Ser Val His Thr Thr Thu Leu Phe Val Wing Wing Wing He Leu 275 280 285 Gly Gln He Wing Phe Gly Leu Glu Gly Thr Leu Wing Val Val Wing He 290 295 300 Phe Leu Wing Val Thr Val Phe Gly Gly Wing Tyr Gly Wing Leu Pro Thr 305 310 315 320 Leu Gly Thr Thr He Phe Leu His Wing Gly Arg Asp His Pro Asp Thr 325 330 335 Wing Being Ser He Tyr Val Val Thr Tyr Gln Val Gly He Wing Ser Gly 340 345 350 Wing Wing Leu Gly Wing Met Wing Val Asp Wing Asp Trp Val Wing Gly Thr 355 360 365 Leu Trp He Met Wing Gly Leu Ser Leu Wing Being Thr Leu Ala Leu Wing 370 375 380 Leu Trp Ser Arg Pro Leu Leu Lys 385 390 < 210 > 135 < 211 > 420 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (397) < 223 > RXN01984 < 400 > 135 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa gtacgcacat ttgttgtctg 60 caatagtgcc ggtgagggag ctgtccgata ttgtgcttac atg cac gaa tct gga 115 Met His Glu Ser Gly 1 5 aaa aat ect gtc aag gtt gtc gac tcg cag gca cca caa gga cgc ggt 163 Lys Asn Pro Val Lys Val Val Asp Be Gln Ala Pro Gln Gly Arg Gly 10 15 20 ggg cat atc ggc gga cat atc aaa cgc cgc ccg att ect agg caa acg 211 Gly His He Gly Gly His He Lys Arg Arg Pro He Pro Arg Gln Thr 25 30 35 gaa att tcc gag gtt cgt cga tat atc gtc atg act gcc ctc gca ctc 259 Glu He Ser Glu Val Arg Arg Tyr He Val Met Thr Ala Leu Ala Leu 40 45 50 ggt ggc tcc gcc atc ggt gtg acg gaa ttt gtc tcc atg ggt ctg ctc 307 Gly Gly Phe Wing He Gly Val Thr Glu Phe Val Ser Met Gly Leu Leu 55 60 65 age gcg atc gcc tcc gac ttt gag atc tcc gaa gac caa gcc gga cac 355 Wing Wing Wing Being Asp Phe Glu He Ser Glu Asp Gln Wing Gly His 70 75 80 85 atc atc acc atc tac gcc ctc gcg tgg ttg tgg gtg ccc cgc 397 He He Thr He Tyr Ala Leu Wing Trp Leu Trp Val Pro Arg 90 95 tgatcacage gtttaccggc aaa 420 < 210 > 136 < 211 > 99 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 136 Met His Glu Ser Gly Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala 1 5 10 15 Pro Gln Gly Arg Gly Gly His He Gly Gly His He Lys Arg Arg Pro 20 25 30 He Pro Arg Gln Thr Glu He Ser Glu Val Arg Arg Tyr He Val Met 35 40 45 Thr Ala Leu Ala Leu Gly Gly Phe Wing He Gly Val Thr Glu Phe Val 50 55 60 Ser Met Gly Leu Leu Ser Ala He Wing Ser Asp Phe Glu He Ser Glu 65 70 75 80 Asp Gln Ala Gly His He He Thr He Tyr Ala Leu Ala Trp Leu Trp 85 90 95 Val Pro Arg < 210 > 137 < 211 > 379 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (379) < 223 > FRXA01984 < 400 > 137 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa gtacgcacat ttgttgtctg 60 caatagtgcc ggtgagggag ctgtccgata ttgtgcttac atg cac gaa tct gga 115 Met His Glu Ser Gly 1 5 aaa aat ect gtc aag gtt gtc gac tcg cag gca cca caa gga cgc ggt 163 Lys Asn Pro Val Lys Val Val Asp Ser Gln Ala Pro Gln Gly Arg Gly 10 15 20 ggg cat atc ggc gga cat atc aaa cgc cgc ccg att ect agg caa acg 211 Gly His He Gly Gly His He Lys Arg Arg Pro He Pro Arg Gln Thr 25 30 35 gaa att tcc gag gtt cgt cga tat atc gtc atg act gcc ctc gca ctc 259 Glu He Ser Glu Val Arg Arg Tyr He Val Met Thr Ala Leu Ala Leu 40 45 50 ggt ggc ttc gcc atc ggt gtg acg gaa ttt gtc tcc atg ggt ctg ctc 307 Gly Gly Phe Ala He Gly Val Thr Glu Phe Val Ser Met Gly Leu Leu 55 60 65 age gcg atc gcc tcc gac ttt gag atc tcc gaa gac cac gcc gga cac 355 Ser Ala He Ala Ser Asp Phe Glu He Ser Glu Asp Gln Ala Gly His 70 75 80 85 atc atc acc atc tac gcc ctc gcg 379 He He Thr He Tyr Ala Leu Wing 90 < 210 > 138 < 211 > 93 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 138 Met His Glu Ser Gly Lys Asn Pro Val Lys Val Val Asp Ser Gln Wing 1 5 10 15 Pro Gln Gly Arg Gly Gly His He Gly Gly His He Lys Arg Arg Pro 20 25 30 He Pro Arg Gln Thr Glu He Ser Glu Val Arg Arg Tyr He Val Met 35 40 45 Thr Ala Leu Ala Leu Gly Gly Phe Wing He Gly Val Thr Glu Phe Val 50 55 60 Ser Met Gly Leu Leu Ser Ala He Wing Ser Asp Phe Glu He Ser Glu 65 70 75 80 Asp Gln Ala Gly His He He Thr He Tyr Ala Leu Wing 85 90 < 210 > 139 < 211 > 735 < 212 > DNA < 213 > Corynebacterium gluta icum < 220 > < 221 > CDS < 222 > (101) .. (712) < 223 > RXA00109 < 400 > 139 aagtggggga agatttcgac aactaaccgg gcgcaaagat gaaactaatg cgtccgacca 60 cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gtg gct tea gag aag 115 Val Ala Ser Glu Lys 1 5 aat cta aaa ttg cgt acc ttg gcg gca gct gct ggg gtg tgg ggc gtt 163 Asn Leu Lys Leu Arg Thr Leu Ala Wing Wing Wing Gly Val Leu Gly Val 10 15 20 ggc gcg atg tcg atg ctc gtg gct gct cg gct gct gcc cat gat gtg 211 Gly Wing Met Ser Met Leu Val Wing Pro Wing Gln Wing Wing His Asp Val 25 30 35 gtg gtg gat tct aat ect gaa aat ggc agt gtc gtt gat gag tcc ccg 259 Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val Val Asp Glu Phe Pro 40 45 50 gag acc att gag ttg gag ttt tcc ggt att ect cag gat ctg ttc here 307 Glu Thr He Glu Leu Glu Phe Ser Gly He Pro Gln Asp Leu Phe Thr 55 60 65 here gtt gca ttg age aat gcg gat tcc gga gag gtg tta act tct gga 355 Thr Val Ala Leu Ser Asn Ala Asp Ser Gly Glu Val Leu Thr Ser Gly 70 75 80 85 act ect cag ctt gag ggg cag cac ttg age tat gaa gtg cca tct gat 403 Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr Glu Val P Ro Be Asp 90 95 100 gtg cag ac g gg ggt aac tac att ttg ggt tcc cag atc actct 451 Val Gln Thr Gly Wing Gly Asn Tyr He Leu Gly Phe Gln He Thr Ser 105 110 115 tct gat ggt cac gct act aaa ggt tea atc tct ttt gag gtg here ggc 499 Ser Asp Gly His Wing Thr Lys Gly Ser He Ser Phe Glu Val Thr Gly 120 125 130 tct gct gag acg here here gag here gca gag acg here act gag tea 547 Ser Ala Glu Thr Thr Thr Glu Thr Thr Wing Glu Thr Thr Thr Thr Glu Ser 135 140 145 gca gca acc ac gac acc ga ga acc ga ga ga ga gaga ac gaga 595 Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu Ala Glu Thr Thr Glu 150 155 160 165 act gct gat gaa act tct gga att ect gcg ccg tgg aat tgg gtt ttg 643 Thr Wing Asp Glu Thr Ser Gly He Pro Wing Pro Trp Asn Trp Val Leu 170 175 180 age atc gtg gcg gtg ctt gtt gtt gca agt gcc atc gtc atg atg att 691 Ser He Val Wing Val Leu Val Val Wing Ser Wing Val Met Met Met 185 190 195 gca aag aat cgt aac cag aaa taagagggtt tatteaccat gaa 735 Ala Lys Asn Arg Asn Gln Lys 200 < 210 > 140 < 211 > 204 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 140 Val Ala Ser Glu Lys Asn Leu Lys Leu Arg Thr Leu Wing Wing Wing 1 5 10 15 Gly Val Leu Gly Val Gly Wing Met Met Met Leu Val Wing Pro Gln Wing 20 25 30 Ala Ala His Asp Val Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val 35 40 45 Val Asp Glu Phe Pro Glu Thr He Glu Leu Glu Phe Ser Gly He Pro 50 55 60 Gln Asp Leu Phe Thr Thr Val Wing Leu Ser Asn Wing Asp Ser Gly Glu 65 70 75 80 Val Leu Thr Ser Gly Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr 85 90 95 Glu Val Pro Ser Asp Val Gln Thr Gly Wing Gly Asn Tyr He Leu Gly 100 105 110 Phe Gln He Thr Ser Ser Asp Gly His Wing Thr Lys Gly Ser He Ser 115 120 125 Phe Glu Val Thr Gly Ser Ala Glu Thr Thr Thr Glu Thr Thr Ala Glu 130 135 140 Thr Thr Thr Glu Ser Ala Ala Thr Thr Asp Thr Ser Glu Thr Thr Glu 145 150 155 160 Wing Glu Thr Thr Glu Thr Wing Asp Glu Thr Ser Gly He Pro Wing Pro 165 170 175 Trp Asn Trp Val Leu Ser He Val Wing Val Leu Val Val Wing Wing 180 185 190 He Val Met Met He Wing Lys Asn Arg Asn Gln Lys 195 200 < 210 > 141 < 211 > 735 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (712) < 223 > RXA00109 < 400 > 141 aagtggggga agatttcgac aactaaccgg gcgcaaagat gaaactaatg cgtccgacca 60 cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gtg gct tea gag aag 115 Val Wing Ser Glu Lys 1 5 aat cta aaa ttg cgt acc ttg gcg gca gct gct ggg gtg gt ggc 163 Asn Leu Lys Leu Arg Thr Leu Wing Ala Wing Wing Gly Val Leu Gly Val 10 15 20 ggc gcg atg tcg atg ctc gtg gct cg ggct gct gcc cat gat gtg 211 Gly Wing Met Ser Met Leu Val Wing Pro Gln Wing Wing His Asp Val 25 30 35 gtg gtg gat tct aat ect gaa aat ggc agt gtc gtt gat gag tcc ccg 259 Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val Val Asp Glu Phe Pro 40 45 50 gag acc att gag ttg gag ttt tcc ggt att ect cag gat ctg ttc here 307 Glu Thr He Glu Leu Glu Phe Ser Gly He Pro Gln Asp Leu Phe Thr 55 60 65 here gtt gca ttg age aat gcg gat tcc gga gag gtg tta act tct gga 355 Thr Val Ala Leu Ser Asn Wing Asp Ser Gly Glu Val Leu Thr Ser Gly 70 75 80 85 act ect cag ctt gag ggg cag cac ttg age tat gaa gtg cca tct gat 403 Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr Glu Val Pro Ser Asp 90 95 100 gtg cag acg gg gct ggt aac tac att ttg ggt ttc cag atc act tct 451 Val Gln Thr Gly Wing Gly Asn Tyr He Leu Gly Phe Gln He Thr Ser 105 110 115 tct gat ggt cac gct act aaa ggt tea atc tct ttt gag gtg here ggc 499 Ser Asp Gly His Wing Thr Lys Gly Ser He Be Phe Glu Val Thr Gly 120 125 130 tct gct gag acg here here gag here gca gag acg here act gag tea 547 Ser Ala Glu Thr Thr Thr Glu Thr Thr Thr Glu Thr Thr Thr Glu Ser 135 140 145 gca gca acc act gac acc tea gag acc acc gaa gca gag here act gaa 595 Wing Wing Thr Thr Asp Thr Ser Glu Thr Thr Glu Wing Glu Thr Thr Glu 150 155 160 165 act gct gat gaa act tct gga att ect gcg ccg tgg aat tgg gtt ttg 643 Thr Wing Asp Glu Thr Ser Gly He Pro Wing Pro Trp Asn Trp Val Leu 170 175 180 age atc gtg gcg gtg ctt gtt gtt gca agt gcc atc gtc atg atg att 691 Ser He Val Wing Val Leu Val Val Wing Ser Wing He Val Met Met He 185 190 195 gca aag aat cgt aac cag aaa taagagggtt tatteaccat gaa 735 Ala Lys Asn Arg Asn Gln Lys 200 < 210 > 142 < 211 > 204 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 142 Val Ala Ser Glu Lys Asn Leu Lys Leu Arg Thr Leu Wing Wing Wing 1 5 10 15 Gly Val Leu Gly Val Gly Wing Met Met Met Leu Val Wing Pro Gln Wing 20 25 30 Wing Wing His Asp Val Val Val Asp Ser Asn Pro Glu Asn Gly Ser Val 35 40 45 Val Asp Glu Phe Pro Glu Thr He Glu Leu Glu Phe Ser Gly He Pro 50 55 60 Gln Asp Leu Phe Thr Thr Val Wing Leu Ser Asn Wing Asp Ser Gly Glu 65 70 75 80 Val Leu Thr Ser Gly Thr Pro Gln Leu Glu Gly Gln His Leu Ser Tyr 85 90 95 Glu Val Pro Ser Asp Val Gln Thr Gly Wing Gly Asn Tyr He Leu Gly 100 105 110 Phe Gln He Thr Ser Ser Asp Gly His Wing Thr Lys Gly Ser He Ser 115 120 125 Phe Glu Val Thr Gly Ser Wing Glu Thr Thr Thr Glu Thr Thr Wing Glu 130 135 140 Thr Thr Thr Glu Ser Wing Wing Thr Thr Asp Thr Ser Glu Thr Thr Glu 145 150 155 160 Wing Glu Thr Thr Glu Thr Wing Asp Glu Thr Ser Gly He Pro Wing Pro 165 170 175 Trp Asn Trp Val Leu Ser He Val Wing Val Leu Val Val Wing Ser Wing 180 185 190 He Val Met Met He Wing Lys Asn Arg Asn Gln Lys 195 200 < 210 > 143 < 211 > 864 10 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (841) < 223 > RXA00996 < 400 > 143 atettaagee ttttgtccaa ggctctgagt tcttagtggc ggtatttacc aagaagtaac 60 acctctatct tgcacctgat ctggcgtaga etcataagtt atg age acc gta acg 115 15 Met Ser Thr Val Thr 1 5 gca gtg cag gtc aac ggc cta aaa gtt tcc ata tcg tcc ggt ttt tea 163 Ala Val Gln Val Asn Gly Leu Lys Val Ser He Ser Ser Gly Phe Ser 10 15 20 cgc aag aaa aaa acg atc ttg cat gat ctc gat ttc acc gta gag 211 Arg Lys Lys Thr Lys Thr He Leu His Asp Leu Asp Phe Thr Val Glu • 25 30 '35 acc gga aag atc acg gga ttg ctg ggg cca tcg ggc age ggc aag here 259 20 Thr Gly Lys He Thr Gly Leu Leu Gly Pro Ser Gly Ser Gly Lys Thr 40 45 50 act ttg atg cgc gcg att gtg gga gtg caa aac ttc gac ggc acc ctt 307 Thr Leu Met Arg Wing He Val Gly Val Gln Asn Phe Asp Gly Thr Leu 55 60 65 gag gtg ttt gat cag ccc gca ggt gct gcc tct ctc cgc ggg aaa atc 355 Glu Val Phe Asp Gln Pro Wing Ala Gly Wing Ser Leu Arg Gly Lys He 70 75 80 85 ggc tat gtc acc aac gcc age gta tat cac gat ctg tcg gtg ata 403 25 Gly Tyr Val Thr Gln Asn Ala Ser Val Tyr His Asp Leu Ser Val He & > - • '- - < tiHHfr'rMt- "/ '- 90 95 100 gaa aac ctc aag tat ttc ggg gct ctg gct aaa gga c onc t act cc 451 Glu Asn Leu Lys Tyr Phe Gly Wing Leu Wing Lys Gly Thr Ser Thr Pro 105 110 115 cgc acc ccg gaa aag att ctg gag gtc tta gac atc gca gat ctt gct 499 Arg Thr Pro Glu Lys He Leu Glu Val Leu Asp He Wing Asp Leu Wing 5 120 125 130 • caa cgc caa gta tea here cta tct ggt ggg cag cgc ggc cga gtc tcc 547 Gln Arg Gln Val Ser Thr Leu Ser Gly Gly Gln Arg Gly Arg Val Ser 135 140 145 ctt gga tgt gcg ctt att gcc tea cca gaa ctc ttg gtg atg gat gag 595 Leu Gly Cys Ala Leu He Wing Pro Pro Glu Leu Leu Val Met Asp Glu 150 155 160 165 cca acc gtg ggt ttg gat ccc att acc cgg caa gca ctg tgg gaa gag 643 Pro Thr Val Gly Leu Asp Pro He Thr Arg Gln Wing Leu Trp Glu Glu 10 170 175 180 ttc acc acc atc gca aaa gca ggt gct gga gtg gtt atc tcc agt cac 691 Phe Thr Thr He Wing Lys Wing Gly Wing Gly Val Val He Ser Ser His • 185 190 195 gtg ttg gag gac gcc gcg cgg tgc gac aac ctc att ttg ttg cgt gat 739 Val Leu Glu Glu Wing Wing Arg Cys Asp Asn Leu He Leu Leu Arg Asp 200 205 210 ggt cgg atc atc tgg agg gga here ccc ccc ctt cta cta gaa gat here 787 Gly Arg He He Trp Arg Gly Thr Pro Thr Arg Leu Leu Glu Asp Thr 15 215 220 225 ggc aaa age tea tac gaa gat gct ttc ttg gct gcc att gac ggg gta 835 Gly Lys Ser Ser Tyr Glu Asp Wing Phe Leu Wing Wing Asp Gly Val 230 235 240 245 agg tea tgaaccctca ctatctgctt gcc 864 Arg Ser • < 210 > 144 20 < 211 > 247 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 144 Met Ser Thr Val Thr Ala Val Gln Val Asn Gly Leu Lys Val Ser He 1 5 10 15 Ser Ser Gly Phe Ser Arg Lys Lys Thr Lys Thr He Leu His Asp Leu 20 25 30 Asp Phe Thr Val Glu Thr Gly Lys He Thr Gly Leu Leu Gly Pro Ser 25 35 40 45 Gly Ser Gly Lys Thr Thr Leu Met Arg Wing He Val Gly Val Gln Asn 50 55 60 Phe Asp Gly Thr Leu Glu Val Phe Asp Gln Pro Wing Gly Ala Wing Ser 65 70 75 80 Leu Arg Gly Lys He Gly Tyr Val Thr Gln Asn Wing Ser Val Tyr His 85 90 95 Asp Leu Ser Val He Glu Asn Leu Lys Tyr Phe Gly Wing Leu Wing Lys 100 • 105 110 Gly Thr Ser Thr Pro Arg Thr Pro Glu Lys He Leu Glu Val Leu Asp 115 120 125 He Wing Asp Leu Wing Gln Arg Gln Val Ser Thr Leu Ser Gly Gly Gln 130 135 140 Arg Gly Arg Val Ser Leu Gly Cys Ala Leu He Wing Ser Pro Glu Leu 145 150 155 160 Leu Val Met Asp Glu Pro Thr Val Gly Leu Asp Pro He Thr Arg Glr. 165 170 175 Ala Leu Trp Glu Glu Phe Thr Thr He Wing Lys Wing Gly Wing Gly Val 180 185 190 Val He Ser Ser His Val Leu Glu Glu Wing Wing Arg Cys Asp Asn Leu 195 200 205 He Leu Leu Arg Asp Gly Arg He He Trp Arg Gly Thr Pro Thr Arg 210 215 220 Leu Leu Glu Asp Thr Gly Lys Ser Ser Tyr Glu Asp Ala Phe Leu Ala 225 230 235 240 Ala He Asp Gly Val Arg Ser 245 < 210 > 145 < 211 > 2463 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (2440) < 223 > RXN00829 < 400 > 145 tgttttagcc atggacccca tactagggag agttttgttt tggtgctaga aaaggttcac 60 caagcgcgaa caggcctatg caaacggtac gatatgacac atg caa aaa gct gat 115 Met Gln Lys Ala Asp 1 5 tcc cat gat tgg att tcg gtc cac ggt gcg aat gaa aac aac ctc aaa 163 Ser His Asp Trp He Ser Val His Gly Ala Asn Glu Asn Asn Leu Lys 10 15 20 aat gtg tcg gtg cgc atc ect aaa agg cgt ctc acc gtg ttc acg ggt 211 Asn Val Ser Val Arg He Pro Lys Arg Arg Leu Thr Val Phe Thr Gly 25 30 35 gtg tcg gga tct ggc aag tcc tcg ctg gtg ttc ggc here att gct gcg 259 Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe Gly Thr He Wing Wing 40 45 50 gaa tea cgc cgg ttg atc aac gaa acc tat age act ttt gtg caa ggt 307 Glu Ser Arg Arg Leu He Asn Glu Thr Tyr Ser Thr Phe Val Gln Gly 55 60 65 ttc atg ccg tcg atg gca agg ccc gat gtt gac cat ttg gaa ggc atc 355 Phe Met Pro Ser Met Wing Arg Pro Asp Val Asp His Leu Glu Gly He 70 75 80 85 acc acg gcg atc atc gtc gat cag gag cag atg ggc gca aac cca cgg 403 Thr Thr Ala He He Val Asp Gln Glu Gln Met Gly Ala Asn Pr o Arg 90 95 100 tct acg gtg ggt g ac gca ac g ac action gcg atg tgc att ttg 451 Be Thr Val Gly Thr Ala Thr Asp Wing Thr Wing Met Leu Arg He Leu 105 110 115 ttt tcc cga atc gcg gaa ect aac gcg ggt ggc ggc ggc gg gct tat tcc 499 Phe Ser Arg He Wing Glu Pro Asn Wing Gly Gly Pro Gly Wing Tyr Ser 120 125 130 ttc aac gtc ccc tct gtt tcc gcc tcc ggc gcc atc acg gtg gaa aag 547 Phe Asn Val Pro Ser Val Be Wing Be Gly Wing He Thr Val Glu Lys 135 140 145 ggc gga aac acc aag cgg gag aaa gct acc ttc aaa cgc acg ggt ggc 595 Gly Gly Asn Thr Lys Arg Glu Lys Wing Thr Phe Lys Arg Thr Gly Gly 150 155 160 165 atg tgc cca gcg tgc gag ggc atg ggc agg gcc tea gac atc gac ctc 643 Met Cys Pro Wing Cys Glu Gly Met Gly Arg Wing Being Asp He Asp Leu 170 175 180 aaa gag ctt ttc gac gcc tcc ctc tcc ctc aac gac gcc gcc ctg acc 691 Lys Glu Leu Phe Asp Wing Ser Leu Ser Leu Asn Asp Gly Ala Leu Thr 185 190 195 atc ccc ggt tac acc cca ggt gga tgg agt tat cgg atg tat tea gaa 739 He Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr Arg Met Tyr Ser Glu 200 205 210 tcg ggc ctt ttt gat gct gcc aag ccg att aag gat ttc acc gag gaa 787 Ser Gly Leu Phe Asp Wing Wing Lys Pro He Lys Asp Phe Thr Glu Glu 215 220 225 gaa cgc cac aac ttc ctt tat ctt gag ccc acc aag atg aag atc gct 835 Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr Lys Met Lys He Wing 230 235 240 245 ggc atc aac atg acc tat gag ggt ctt atc ccc cgc att cag aaa tcc 883 Gly He Asn Met Thr Tyr Glu Gly Leu He Pro Arg He Gln Lys Ser 250 255 260 atg ttg tct aag gat cgc gag ggc atg cag aag cat att cgt gcg ttc 931 Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys His He Arg Wing Phe 265 270 275 gtg gat cgc gcg gtt acc ttc att ect tgc ec t gcg tgt ggt gga act 979 Val Asp Arg Wing Val Thr Phe He Pro Cys Pro Wing Cys Gly Gly Thr 280 285 290 cga tta gcg cca cat gcc ttg gag tcc aag atc aat ggc aaa aac atc 1027 Arg Leu Ala Pro His Ala Leu Glu Ser Lys He Asn Gly Lys Asn He 295 300 305 gct gag ttg tgc gcg atg gag gtc cgt gat ttg gcc aag tgg atc aaa 1075 Wing Glu Leu Cys Wing Met Glu Val Arg Asp Leu Wing Lys Trp He Lys 310 315 320 325 acg gtg gaa gcc ccc tcg gtt gct ccc ctg ctc acc gca ctg act gaa 1123 Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu Thr Ala Leu Thr Glu 330 335 340 acc ctg gat aac ttc gtg gag atc ggt ttg ggc tat atc caa ctc gat 1171 Thr Leu Asp Asn Phe Val Glu He Gly Leu Gly Tyr He Gln Leu Asp 345 350 355 cgc ccc gct ggc acg ttg tct ggt ggt gag gca cag cgc acc aag atg 1219 Arg Pro Wing Gly Thr Leu Ser Gly Gly Glu Wing Gln Arg Thr Lys Met 360 365 370 atc cgc cat ttg ggc tct gca ttg act gc gtc gtc act tat gtt ttt gat 1267 He Arg His Leu Gly Be Wing Leu Thr Asp Val Thr Tyr Val Phe Asp 375 380 385 gaa ccc acc gcc ggt ttg ca c gcc tac gac att gaa cgc atg aac aag 1315 Glu Pro Thr Wing Gly Leu His Wing Tyr Asp He Glu Arg Met Asn Lys 390 395 400 405 ttg ctc ctc gat ctt cgc gat aaa ggc aat acc gtt tta gtc gtg gag 1363 Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr Val Leu Val Val Glu 410 415 420 cac aag ccg gaa acc atc gcc att gca gat cat gtg gtg gac ctt ggg 1411 His Lys Pro Glu Thr He Wing He Wing Asp His Val Val Asp Leu Gly 425 430 435 cca ggt gca ggc gcg ggt gga ggt gaa att cgg ttt gag ggg age gtc 1459 Pro Gly Wing Gly Wing Gly Gly Gly Glu He Arg Phe Glu Gly Ser Val 440 445 450 gac aag ctt aaa gac age gac acc gtg act ggc ctc cat ttt aat gac 1507 Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly Leu His Phe Asn Asp 455 460 465 'cgg gcg tea ttg aag gaa tcc gtg cgt gcg ccg cat ggc gcc ctg gag 1555 Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro His Gly Ala Leu Glu 470 475 480 485 atc cgc ggg gcc gat cga aat aat ttg aac aat gtg gat gtc gat 1603 He Arg Gly Wing Asp Arg Asn Asn Leu Asn Asn Val Asp Val Asp He 490 495 500 ccg ctc ggc gtg ttc acg gcg att tcc ggc gtt gca ggt tcg ggt aag 1651 Pro Leu Gly Val Phe Thr Ala He Ser Gly Val Ala Gly Ser Gly Lys 505 510 515 tcc tcg ttg att cat gag att ccg cgt gat gag tcg gtt gtg ttt gtc 1699 Be Ser Leu He His Glu He Pro Arg Asp Glu Be Val Val Phe Val 520 525 530 gat caa acc gca atc cac ggt tct aat cct tct aat ect gcg here tat 1747 Asp Gln Thr Ala He His Gly Ser Asn Arg Ser Asn Pro Ala Thr Tyr 535 540 545 here ggc atg ctg gat tcg att cgc aag gct ttt gcc aag gcc aat gat 1795 Thr Gly Met Leu Asp Ser He Arg Lys Ala Phe Ala Lys Ala Asn Asp 550 555 560 565 gtg aaa ccg gcg ctg ttc tcc ccc aat tct gaa ggc gcg tgc cca aac 1843 Val Lys Pro Ala Leu Phe Ser Pro Asn Ser Glu Gly Ala Cys Pro Asn 570 575 580 tgt aag ggc gcc ggc tcg gtc tat gtc gat ttg ggc atg atg gct ggg 1891 Cys Lys Gly Wing Gly Ser Val Tyr Val Asp Leu Gly Met Met Wing Gly 585 590 595 gta tct tcg ccg tgt gag gtg tgc gag ggc aag cgt ttt gat gag tcc 1939 Val Ser Ser Pro Cys Glu Val Cys Glu Gly Lys Arg Phe Asp Glu Ser 600 605 610 gtg ttg gac tac cac ttt ggt ggc aag gac atc gca gac gtg ttg ggg 1987 Val Leu Asp Tyr His Phe Gly Gly Lys Asp He Wing Asp Val Leu Gly 615 620 625 ctg tcg gct gcc aat gcg tat gag ttt tcc gcg gcg aaa gat tea aag 2035 Leu Ser Ala Ala Asn Ala Tyr Glu Phe Phe Ala Ala Lys Asp Ser Lys 630 635 640 645 att ttg ect gcg gca aag atc gca aag agg ctt gtc gac gtc ggc ctc 2083 He Leu Pro Ala Ala Lys He Ala Lys Arg Leu Val Asp Val Gly Leu 650 655 660 ggc tac atc acc ctc ggc cag ccg ctc acc acg ttg tcc ggc ggt gaa 2131 Gly Tyr He Thr Leu Gly Gln Pro Leu Thr Thr Leu Ser Gly Gly Glu 665 670 675 cgc cag cgt ttg aag ctc gcc acc cac atg gca gac aag gcc acc acc 2179 Arg Gln Arg Leu Lys Leu Wing Thr His Met Wing Asp Lys Wing Thr Thr 680 685 690 ttt att ttg gat gag ccc acc here ggc ctg cac ctc gct gat gtg aaa 2227 Phe He Leu Asp Glu Pro Thr Thr Gly Leu His Leu Wing Asp Val Lys 695 700 705 acc ttg ctg gat ctt ttt gat caa ctg gtt gat gac ggc aag tct gtc 2275 Thr Leu Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val 710 715 720 725 atc gtc atc gac cac cac ctc ggc gtg ctc gct cac gct gac cac atc 2323 He Val He Glu His His Leu Gly Val Leu Wing His Wing Asp His He 730 735 740 att gat gtc ggc ect ggt gca ggt tct gat ggt ggc tcg att gta ttc 2371 He Asp Val Gly Pro Gly Wing Gly Ser Asp Gly Gly Ser He Val Phe 745 750 755 gag ggc age ccc gcg gaa ctc atc aaa act gat act cca here gga cgc 2419 Glu Gly Ser Pro Wing Glu Leu He Lys Thr Asp Thr Pro Thr Gly Arg 760 765 770 cac ctt aaa gct tat gta gat tagtttctta tggaaaaccc t gg 2463 His Leu Lys Ala Tyr Val Asp 775 780 < 210 > 146 < 211 > 780 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 146 Met Gln Lys Wing Asp Ser His Asp Trp He Ser Val His Gly Wing Asn 1 5 10 15 Glu Asn Asn Leu Lys Asn Val Ser Val Arg He Pro Lys Arg Arg Leu 20 25 30 Thr Val Phe Thr Gly Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe 35 40 45 Gly Thr He Wing Wing Glu Ser Arg Arg Leu He Asn Glu Thr Tyr Ser 50 55 60 Thr Phe Val Gln Gly Phe Met Pro Ser Met Wing Arg Pro Asp Val Asp 65 70 75 80 His Leu Glu Gly He Thr Thr Wing He He Val Asp Gln Glu Gln Met 85 90 95 Gly Wing Asn Pro Arg Ser Thr Val Gly Thr Wing Thr Asp Wing Thr Wing 100 105 110 Met Leu Arg He Leu Phe Ser Arg He Wing Glu Pro Asn Wing Gly Gly 115 120 125 Pro Gly Ala Tyr Ser Phe Asn Val Pro Ser Val Ser Ala Ser Gly Wing 130 135 140 He Thr Val Glu Lys Gly Gly Asn Thr Lys Arg Glu Lys Wing Thr Phe 145 150 155 160 Lys Arg Thr Gly Gly Met Cys Pro Wing Cys Glu Gly Met Gly Arg Wing 165 170 175 Being Asp He Asp Leu Lys Glu Leu Phe Asp Wing Being Leu Ser Leu Asn 180 185 190 Asp Gly Wing Leu Thr He Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr 195 200 205 Arg Met Tyr Ser Glu Ser Gly Leu Phe Asp Wing Wing Lys Pro He Lys 210 215 220 Asp Phe Thr Glu Glu Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr 225 230 235 240 10 Lys Met Lys He Wing Gly He Asn Met Thr Tyr Glu Gly Leu He Pro 245 250 255 Arg He Gln Lys Ser Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys 260 265 270 His He Arg Ala Phe Val Asp Arg Ala Val Thr Phe He Pro Cys Pro 275 280 285 Wing Cys Gly Gly Thr Arg Leu Wing Pro His Wing Leu Glu Ser Lys He 290 295 300 15 Asn Gly Lys Asn He Wing Glu Leu Cys Wing Met Glu Val Arg Asp Leu 305 310 315 320 Wing Lys Trp He Lys Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu 325 330 335 Thr Ala Leu Thr Glu Thr Leu Asp Asn Phe Val Glu He Gly Leu Gly 340 345 350 Tyr He Gln Leu Asp Arg Pro Wing Gly Thr Leu Ser Gly Gly Glu Ala 355 360 365 20 Gln Arg Thr Lys Met He Arg His Leu Gly Ser Wing Leu Thr Asp Val 370 375 380 Thr Tyr Val Phe Asp Glu Pro Thr Wing Gly Leu His Wing Tyr Asp He 385 390 395 400 Glu Arg Met Asn Lys Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr 405 410 415 Val Leu Val Val Glu His Lys Pro Glu Thr He Ala He Wing Asp His 420 425 430 25 Val Val Asp Leu Gly Pro Gly Wing Gly Wing Gly Gly Gly Glu He Arg 435 440 445 Phe Glu Gly Ser Val Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly 450 455 460 Leu His Phe Asn Asp Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro 465 470 475 480 His Gly Wing Leu Glu He Arg Gly Wing Asp Arg Asn Asn Leu Asn Asn 485 490 495 Val Asp Val Asp He Pro Leu Gly Val Phe Thr Wing He Ser Gly Val 500 505 510 Wing Gly Ser Gly Lys Ser Ser Leu He His Glu He Pro Arg Asp Glu 515 520 525 Val Val Phe Val Asp Gln Thr Ala He His Gly Ser Asn Arg Ser 530 535 540 Asn Pro Wing Thr Tyr Thr Gly Met Leu Asp Ser He Arg Lys Wing Phe 545 550 555 560 Wing Lys Wing Asn Asp Val Lys Pro Wing Leu Phe Ser Pro Asn Ser Glu 565 570 575 Gly Wing Cys Pro Asn Cys Lys Gly Wing Gly Ser Val Tyr Val Asp Leu 580 585 590 Gly Met Met Wing Gly Val Ser Ser Cys Pro Glu Val Cys Glu Gly Lys 595 600 605 Arg Phe Asp Glu Ser Val Leu Asp Tyr His Phe Gly Gly Lys Asp He 610 615 620 Wing Asp Val Leu Gly Leu Ser Wing Wing Asn Wing Tyr Glu Phe Wing Phe 625 630 635 640 Wing Lys Asp Ser Lys He Leu Pro Wing Wing Lys He Wing Lys Arg Leu 645 650 655 Val Asp Val Gly Leu Gly Tyr He Thr Leu Gly Gln Pro Leu Thr Thr 660 665 670 Leu Ser Gly Glu Arg Gln Arg Leu Lys Leu Wing Thr His Met Wing 675 680 685 Asp Lys Wing Thr Thr Phe He Leu Asp Glu Pro Thr Thr Gly Leu His 690 695 700 Leu Wing Asp Val Lys Thr Leu Leu Asp Leu Phe Asp Gln Leu Val Asp 705 710 715 720 Asp Gly Lys Ser Val He Val He Glu His His Leu Gly Val Leu Wing 725 730 735 His Wing Asp His He He As As Val Gly Pro Gly Wing Gly Ser Asp Gly 740 745 750 Gly Ser He Val Phe Glu Gly Ser Pro Wing Glu Leu He Lys Thr Asp 755 760 765 Thr Pro Thr Gly Arg His Leu Lys Wing Tyr Val Asp 770 775 780 < 210 > 147 < 211 > 278 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (255) < 223 > FRXA00829 < 400 > 147 ttg gat gag ccc acc here ggc ctg cac ctc gct gat gtg aaa acc ttg 48 Leu Asp Glu Pro Thr Thr Gly Leu His Leu Ala Asp Val Lys Thr Leu January 5 10 15 ctg gat ctt ttt gat caa ctg gtt gat gac ggc aag tct gtc atc gtc 96 Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val He Val 20 25 30 atc gaa cac cac ctc ggc gtg ctc gct cac gct gac cac atc att gat 144 He Glu His His Leu Gly Val Leu Ala His Ala Asp His He He Asp 35 40 45 gtc ggc ect ggt gca ggt tct gat ggt ggc tcg att gta ttc gag ggc 192 Val Gly Pro Gly Ala Gly Ser Asp Gly Gly Ser He Val Phe Glu Gly 50 55 60 age ccc gcg gaa ctc atc aaa cca act gat act gga cgc cac ctt here 240 Ser Leu Pro Ala Glu Lys Thr Asp Thr He Pro Arg Gly Thr His Leu 65 70 75 80 aaa gct gta tat gat tgg tagtttctta tggaaaaccc 278 Lys Val Tyr Ala Asp 85 < 210 > 148 < 211 > 85 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 148 Leu Asp Glu Pro Thr Thr Gly Leu His Leu Wing Asp Val Lys Thr Leu 1 5 10 15 Leu Asp Leu Phe Asp Gln Leu Val Asp Asp Gly Lys Ser Val He Val 20 25 30"" - - '"- He Glu His His Leu Gly Val Leu Wing His Wing Asp His He He Asp 35 40 45 Val Gly Pro Gly Wing Gly Ser Asp Gly Gly Ser He Val Phe Glu Gly 50 55 60 Ser Pro Ala Glu Leu He Lys Thr Asp Thr Pro Thr Gly Arg His Leu 65 70 75 80 Lys Ala Tyr Val Asp 85 < 210 > 149 < 211 > 1663 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1663) < 223 > FRXA00834 < 400 > 149 tgttttagcc atggacccca tactagggag agttttgttt ..tggtgctaga aaaggttcac 60 caagcgcgaa caggcctatg caaacggtac gatatgacac atg caa aaa gct gat 115 Met Gln Lys Ala Asp 1 5 tcc cat gat tgg att tcg gtc cac ggt gcg aat gaa aac aac ctc aaa 163 Ser His Asp Trp He Ser Val His Gly Wing Asn Glu Asn Asn Leu Lys 10 15 20 aat gtg tcg gtg cgc atc ect aaa agg cgt ctc acc gtg ttc acg ggt 211 Asn Val Ser Val Arg He Pro Lys Arg Arg Leu Thr Val Phe Thr Gly 25 30 35 gtg tcg gga tct ggc aag tcc tcg ctg gtg ttc ggc here att gct gcg 259 Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe Gly Thr He Wing Wing 40 45 50 gaa tea cgc cgg ttg atc aac gaa acc tat age act ttt gtg caa ggt 307 Glu Ser Arg Arg Leu He Asn Glu Thr Tyr Ser Thr Phe Val Gln Gly 55 60 65 ttc atg ccg tcg atg gca agg ccc gat gtt gac cat ttg gaa ggc atc 355 Phe Met Pro Ser Met Wing Arg Pro Asp Val Asp His Leu Glu Gly He 70 75 80 85 acc acg gcg atc atc gtc gat cag gag cag atg ggc gca aac cca cgg 403 Thr Thr Ala He He Val Asp Gln Glu Gln Met Gly Ala Asn P ro Arg 90 95 100 tct acg gtg ggt g ac gca action gcg action ac gcg atg tgc att ttg 451 Be Thr Val Gly Thr Wing Thr Asp Wing Thr Wing Met Leu Arg He Leu 105 110 115 ttt tcc cga atc gcg gaa ect aac gcg ggt ggc ggc ggc gg gct tat tcc 499 Phe Ser Arg He Wing Glu Pro Asn Wing Gly Gly Pro Gly Wing Tyr Ser 120 125 130 ttc aac gtc ccc tct gtt tcc gccc gcc gcc atc acg gtg gaa aag 547 Phe Asn Val Pro Ser Val Be Wing Be Gly Wing He Thr Val Glu Lys 135 140 145 ggc gga aac acc aag cgg gag aaa gct acc ttc aaa cgc acg ggt ggc 595 Gly Gly Asn Thr Lys Arg Glu Lys Wing Thr Phe Lys Arg Thr Gly Gly 150 155 160 165 atg tgc cca gcg tgc gag ggc atg ggc agg gcc tea gac atc gac ctc 643 Met Cys Pro Wing Cys Glu Gly Met Gly Arg Wing Being Asp He Asp Leu 170 175 180 aaa gag ctt ttc gac gcc tcc ctc tcc ctc aac gac ggc gcc ctg acc 691 Lys Glu Leu Phe Asp Wing Ser Leu Ser Leu Asn Asp Gly Wing Leu Thr 185 190 195 atc ccc ggt tac acc cca ggt ggg tgg agt tat cgg atg tat tea gaa 739 He Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr Arg Met Tyr Ser Glu 200 205 210 tcg ggc ctt ttt gat gct gcc aag ccg att aag gat ttc acc gag gaa 787 Ser Gly Leu Phe Asp Wing Wing Lys Pro He Lys Asp Phe Thr Glu Glu 215 220 225 gaa cgc cac aac ttc ctt tat ctt gag ccc acc aag ag atc gct 835 Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr Lys Met Lys He Wing 230 235 240 245 ggc atc aac atg acc tat gag ggt ctt atc ccc cgc att cag aaa tcc 883 Gly He Asn Met Thr Tyr Glu Gly Leu He Pro Arg He Gln Lys Ser 250 255 260 atg ttg tct aga gat cgc gaa ggc atg cag aag cat att cgt gcg ttc 931 Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys His He Arg Ala Phe 265 270 275 gtg gat cga gcg gtt acc ttc att ect tgc ect gcg tgt ggt gga act 979 Val Asp Arg Wing Val Thr Phe He Pro Cys Pro Wing Cys Gly Gly Thr 280 285 290 cga tta gcg cca cat gcc ttg gag tcc aag atc aat ggc aaa aac atc 1027 Arg Leu Wing Pro His Wing Leu Glu Ser Lys He Asn Gly Lys Asn He 295 300 305 gct gag tg tgc gcg atg gag gtc cgt gat ttg gcc aag tgg atc aaa 1075 Wing Glu Leu Cys Wing Met Glu Val Arg Ace p Leu Wing Lys Trp He Lys 310 315 320 325 acg gtg gaa gcc ccc tcg gtt gct ccc ctg ctc acc gca ctg act gaa 1123 Thr Val Glu Ala Pro Ser Val Ala Pro Leu Leu Thr Ala Leu Thr Glu 330 335 340 acc ctg gat aac ttc gtg gag atc ggt ttg ggc tat atc caa ctc gat 1171 Thr Leu Asp Asn Phe Val Glu He Gly Leu Gly Tyr He Gln Leu Asp 345 350 355 cgc ccc gct ggc acg ttg tct ggt ggt gag gca cag cgc acc aag atg 1219 Arg Pro Wing Gly Thr Leu Ser Gly Gly Glu Wing Gln Arg Thr Lys Met 360 365 370 atc cgc cat ttg ggc tct gca ttg act gc gc gtc acc tat gtt ttt gat 1267 He Arg His Leu Gly Be Wing Leu Thr Asp Val Thr Tyr Val Phe Asp 375 380 385 gaa ccc acc gcc ggt tcg cac gcc tac gac att gaa cgc atg aac aag 1315 Glu Pro Thr Wing Gly Leu His Wing Tyr Asp He Glu Arg Met Asn Lys 390 395 400 405 ttg ctg ctc gat ctt cgc gat aaa ggc aat acc gtt tta gtc gtg gag 1363 Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr Val Leu Val Val Glu 410 415 420 cac aag ccg gaa acc atc gcc att gca gat cat gtg gtg gac ctt ggg 1411 His Lys Pro Glu Thr He Wing He Wing Asp His Val Val Asp Leu Gly 425 430 435 cca ggt gca ggc gcg ggt gga ggt gaa att cgg ttt gag ggg age gtc 1459 Pro Gly Wing Gly Wing Gly Gly Gly Glu He Arg Phe Glu Gly Ser Val 440 445 450 gac aag ctt aaa gac age gac acc gtg act ggc ctc cat ttt aat gac 1507 Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly Leu His Phe Asn Asp 455 460 465 cgg gcg tea ttg aag gaa tc gtg cgt gcg ccg cat ggc gcc gtc gag 1555 Arg Wing Ser Leu Lys Glu Ser Val Arg Wing Pro His Gly Wing Leu Glu 470 475 480 485 atc cgc ggg gcc gat cga aat aat ttg aac aat gtg gat gtc gat 1603 He Arg Gly Wing Asp Arg Asn Asn Leu Asn Asn Val Asp Val Asp He 490 495 500 ccg ctc ggc gtg ttc acg gcg att tcc ggc gtt gca ggt tcg ggt aag 1651 Pro Leu Gly Val Phe Thr Ala He Ser Gly Val Ala Gly Ser Gly Lys 505 510 515 t cc tcg ttg att 1663 Ser Ser Leu He 520 < 210 > 150 < 211 > 521 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 150 Met Gln Lys Wing Asp Ser His Asp Trp He Ser Val His Gly Wing Asn 10 15 Glu Asn Asn Leu Lys Asn Val Ser Val Arg He Pro Lys Arg Arg Leu 20 25 30 Thr Val Phe Thr Gly Val Ser Gly Ser Gly Lys Ser Ser Leu Val Phe 35 40 45 5 Gly Thr He Wing Wing Glu Ser Arg Arg Leu He Asn Glu Thr Tyr Ser 50 55 60 Thr Phe Val Gln Gly Phe Met Pro Ser Met Wing Arg Pro Asp Val Asp 65 70 75 80 His Leu Glu Gly He Thr Thr Wing He Hep Val Asp Gln Glu Gln Met 85 90 95 Gly Wing Asn Pro Arg Ser Thr Val Gly Thr Wing Thr Asp Wing Thr Wing 100 105 110 10 Met Leu Arg He Leu Phe Ser Arg He Wing Glu Pro Asn Wing Gly Gly 115 120 125 Pro Gly Ala Tyr Ser Phe Asn Val Pro Ser Val Ser Ala Ser Gly Wing 130 135 140 He Thr Val Glu Lys Gly Gly Asn Thr Lys Arg Glu Lys Wing Thr Phe 145 150 155 160 Lys Arg Thr Gly Gly Met Cys Pro Wing Cys Glu Gly Met Gly Arg Wing 165 170 175 15 Being Asp He Asp Leu Lys Glu Leu Phe Asp Wing Being Leu Ser Leu Asn 180 185 190 Asp Gly Wing Leu Thr He Pro Gly Tyr Thr Pro Gly Gly Trp Ser Tyr 195 200 205 Arg Met Tyr Ser Glu Ser Gly Leu Phe Asp Wing Wing Lys Pro He Lys 210 215 220 Asp Phe Thr Glu Glu Glu Arg His Asn Phe Leu Tyr Leu Glu Pro Thr 225 230 235 240 20 Lys Met Lys He Wing Gly He Asn Met Thr Tyr Glu Gly Leu He Pro 245 250 255 Arg He Gln Lys Ser Met Leu Ser Lys Asp Arg Glu Gly Met Gln Lys 260 265 270 His He Arg Ala Phe Val Asp Arg Ala Val Thr Phe He Pro Cys Pro 275 280 285 Ala Cys Gly Gly Thr Arg Leu Wing Pro His Wing Leu Glu Being Lys He 290 295 300 25 Asn Gly Lys Asn He Wing Wing Glu Leu Cys Wing Met Glu Val Arg Asp Leu ? r? \ - SítiÍ (ífm t? ~ * Tua * * »• * * •. -, - ~ ^ - ^ - - * '- - * ^" ^ 305 310 315 320 Wing Lys Trp He Lys Thr Val Glu Wing Pro Ser Val Wing Pro Leu Leu 325 330 335 Thr Wing Leu Thr Glu Thr Leu Asp Asn Phe Val Glu He Gly Leu Gly 340 345 350 Tyr He Gln Leu Asp Arg Pro Wing Gly Thr Leu Ser Gly Gly Glu Wing 355 360 365 Gln Arg Thr Lys Met He Arg His Leu Gly Ser Wing Leu Thr Asp Val 370 375 380 Thr Tyr Val Phe Asp Glu Pro Thr Wing Gly Leu His Wing Tyr Asp He 385 390 395 400 Glu Arg Met Asn Lys Leu Leu Leu Asp Leu Arg Asp Lys Gly Asn Thr 405 410 415 Val Leu Val Val Glu His Lys Pro Glu Thr He Wing He Wing Asp His 420 425 430 Val Val Asp Leu Gly Pro Gly Wing Gly Wing Gly Gly Gly Glu He Arg 435 440 445 Phe Glu Gly Ser Val Asp Lys Leu Lys Asp Ser Asp Thr Val Thr Gly 450 455 460 Leu His Phe Asn Asg Arg Ala Ser Leu Lys Glu Ser Val Arg Ala Pro 465 470 475 480 His Gly Ala Leu Glu He Arg Gly Wing Asp Arg Asn Asn Leu Asn Asn 485 490 495 Val Asp Val Asp He Pro Leu Gly Val Phe Thr Ala He Ser Gly Val 500 505 510 Wing Gly Ser Gly Lys Ser Ser Leu He 515 520 < 210 > 151 < 211 > 864 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (841) < 223 > RXA00995 < 400 > 151 gtcggatcat ctggagggga acacccacac gccttctaga agatacaggc aaaagctcat 60 acgaagatgc tttcttggct gccattgacg gggtaaggtc atg aac ect cac tat 115 Met Asn Pro His Tyr ctg ctt gcc acg gtc aaa cga gtc ctg ctg cag ctg aaa gcc gat aaa 163 Leu Leu Ala Thr Val Lys Arg Val Leu Leu Gln Leu Lys Wing Asp Lys 10 15 20 cgt tcc atc gcg ctg att ctt cta gca ccc gtg gcg tcg atg tcg ctg 211 Arg Ser He Ala Leu He Leu Leu Ala Pro Val Ala Leu Met Ser Leu 25 30 35 ttt tat tac atg tat tcc tcc here ccg gca ggc acc cag ctg ttt aag 259 Phe Tyr Tyr Met Tyr Ser Ser Thr Pro Wing Gly Thr Gln Leu Phe Lys 40 45 50 acc att tcc acg gtc atg atc gca gtg ttc ccc ttg atg ctc atg ttt 307 Thr He Be Thr Val Met He Wing Val Phe Pro Leu Met Leu Met Phe 55 60 65 ttg atg acg tcg gtg acg atg cag aga gaa cgc aac gct gga acg ctc 355 Leu Met Thr Ser Val Thr Met Gln Arg Glu Arg Asn Ala Gly Thr Leu 70 75 80 85 gag cgc ttg tgg acc acg aac att cac cgc gtt gat ttg atc ggt ggc 403 Glu Arg Leu Trp Thr Thr Asn He His Arg Val Asp Leu He Gly Gl and 90 95 100 tac ggg gtg gcc ttc ggc atc atg gcg gtg gcg caa tct ttg ctc atg 451 Tyr Gly Val Ala Phe Gly He Met Ala Ala Ala Gln Ser Leu Leu Met 105 110 115 gtg ctc acc ctt cgg tat ctc ctg ggt gtg gaa acc gaa tcg gag tgg 499 Val Leu Thr Leu Arg Tyr Leu Leu Gly Val Glu Thr Glu Ser Glu Trp 120 125 130 tgg att tct acg ctc att gct gcg atc acc ggt ctt atc gga gtg tct 547 Trp He Ser Thr Leu He Wing Wing He Thr Gly Leu He Gly Val Ser 135 140 145 ctt ggc ctg ttg age tct gcg ttt gcc age act gag ttc caa gct atc 595 Leu Gly Leu Leu Ser Be Ala Phe Wing Be Thr Glu Phe Gln Wing 150 155 160 165 caa acg ctg ccg ttg ctt att ttg ccc cag ttc cta ttg tgc ggt ttg 643 Gln Thr Leu Pro Leu Leu He Leu Pro Gln Phe Leu Leu Cys Gly Leu 170 175 180 ctg atc cca cgg gat gat ctg gat gtg ttg cgc tgg gtt tct aat 691 Leu He Pro Arg Asp Asp Leu Pro Asp Val Leu Arg Trp Val Ser Asn 185 190 195 gtg ttg ccg ctg tcc tat gca gtt gat gca gcg ctt gag gcc tea cgg 739 Val Leu Pro Leu Ser Tyr Ala Val Asp Ala Ala Leu Glu Ala Ser Arg 200 205 210 acg gga atc gga cag caa gta gtg gtc aac att gcc atc tgc gcc gcg 787 Thr Gly He Gly Gln Gln Val Val Val Asn He Wing He Cys Wing Wing 215 220 225 ttt gcc gtg age ttc ctg ctg gtg gcg gcg cta tcg atg ccg aga atg 835 Phe Ala Val Ser Phe Leu Leu Val Ala Ala Leu Ser Met Pro Arg Met 230 235 240 245 acc cgc tagattaetc ttccagcgag gtg 864 Thr Arg < 210 > 152 < 211 > 247 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 152 Met Asn 'Pro His Tyr Leu Leu Wing Thr Val Lys Arg Val Leu Leu Gln 1 5 10 15 Leu Lys Wing Asp Lys Arg Ser He Wing Leu He Leu Leu Wing Pro Val 20 25 30 Wing Leu Met Being Leu Phe Tyr Tyr Met Tyr Ser Ser Thr Pro Wing Gly 35 40 45 Thr Gln Leu Phe Lys Thr He Ser Thr Val Met He Wing Val Phe Pro 50 55 60 Leu Met Leu Met Phe Leu Met Thr Ser Val Thr Met Gln Arg Glu Arg 65 70 75 80 Asn Wing Gly Thr Leu Glu Arg Leu Trp Thr Thr Asn He His Arg Val 85 90 95 Asp Leu He Gly Gly Tyr Gly Val Wing Phe Gly He Met Wing Val Wing 100 105 110 Gln Ser Leu Leu Met Val Leu Thr Leu Arg Tyr Leu Leu Gly Val Glu 115 120 125 Thr Glu Ser Glu Trp Trp He Ser Thr Leu He Ala Wing He Thr Gly 130 135 140 Leu He Gly Val Ser Leu Gly Leu Leu Ser Ser Ala Phe Ala Ser Thr 145 150 155 160 Glu Phe Gln Wing He Gln Thr Leu Pro Leu Leu He Leu Pro Gln Phe 165 170 175 Leu Leu Cys Gly Leu Leu He Pro Arg Asp Asu Leu Pro Asp Val Leu 180 185 190 Arg Trp Val Ser Asn Val Leu Pro Leu Ser Tyr Ala Val Asp Wing Wing 195 200 205 Leu Glu Wing Being Arg Thr Gly He Gly Gln Gln Val Val Val Asn He 210 215 220 Wing He Cys Wing Wing Phe Wing Val Being Phe Leu Leu Val Wing Wing Leu 225 230 235 240 Ser Met Pro Arg Met Thr Arg 245 < 210 > 153 < 211 > 1353 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1330) < 223 > RXN00803 < 400 > 153 tcatccttcc ttagctcgcg tgagcttccc aagcgtaagc acccccgtgt gagggcataa 60 10 cggccgttct gttaaagatt ggtctggcca tttcctccat atg ggg gtg ccc gcg 115 Met Gly Val Ser Wing 1 5 ctt aac atg tct gac atg gtg gcg aac aaa cgg ggg cag cgt aaa gtc 163 Leu Asn Met Being Asp Met Val Wing Asn Lys Arg Wing Gln Arg Lys Val 10 15 20 tgg cta gcg gta gct tta tcg gtc ttt acg gtc gcg tgg ggt ggc aat 211 Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val Wing Trp Gly Gly Asn 25 30 35 15 gaa ttc act ccc ttg ctg gtg ttt tac cga ggt gaa ggg ttc ttt age 259 Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly Glu Gly Phe Phe Ser 40 45 50 aac ctg ttc atc gac ctt ttg ctg gtg ttt tat gcc atc gga gta gcg 307 Asn Leu Phe He Asp Leu Leu Leu Val Phe Tyr Ala He Gly Val Ala 55 60 65 gta ggt ttg ctg gca gct gcttct tct tct gac cgc tat ggc cga cgt 355 Val Gly Leu Leu Ala Wing Gly Pro Leu Ser Asp Arg Tyr Gly Arg Arg 70 75 80 85 20 gcc gtc atg ttg ect gcg cca ttg atc gcg atc ttg ggt tcc gcg ttg 403 Wing Val Met Leu Pro Wing Pro Leu He Wing He Leu Gly Wing Wing Leu 90 95 100 att gcc tcg ggt gaa acc gcc atc ctg att gcc att ggt cga gtg 451 He Wing Ser Gly Glu Glu Thr Wing He Leu He Wing He Gly Arg Val 105 110 115 ctg tcg gga att tcg gtg ggc atg gtg atg here gcg gga gtcc tgg 499 Leu Ser Gly He Ser Val Gly Met Val Met Thr Wing Gly Gly Ser Trp 120 125 130 25 - * - - - ^^ 8t > ..., att aag gag ctt tea tcg tcg cgg ttt gag cca ggg gtg aaa acc agt 547 He Lys Glu Leu Being Ser Arg Phe Glu Pro Gly Val Lys Thr Ser 135 140 145 gct ggt gca aaa cgc gca tcg atg tct ttg acc ggt ggt ttt gcg ctc 595 Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr Gly Gly Phe Ala Leu 150 155 160 165 ggc cca gcg ctt gct ggt gtg atg gca cag tgg ctg cca cta ect gga 643 Gly Pro Ala Leu Ala Gly Val Met Ala Gln Trp Leu Pro Leu Pro Gly 170 175 180 cag ttg gca tat gtt ttg cac att att ctc act ctg att ttg ttc ccg 691 Gln Leu Ala Tyr Val Leu His He He Leu Thr Leu He Leu Phe Pro 185 190 195 ttg ctt att here gcg ccg gaa act cgt caa tea gcg cac ctg aaa act 739 Leu Leu He Thr Wing Pro Glu Thr Arg Gln Ser Wing His Leu Lys Thr 200 205 210 10 aag gga tea ttc tgg tea gat gtg ctt gtg cca tct gca cta gac aag 787 Lys Gly Ser Phe Trp Ser Asp Val Leu Val Pro Ser Ala Leu Asp Lys 215 220 225 cga ttc ttg ttt gtg gtt gct cca att gga ccg tgg gtt ttc ggt gcg 835 Arg Phe Leu Phe Val Val Ala Pro He Gly Pro Trp Val Phe Gly Wing 230 235 240 245 gcc ttc act gcc tac gca gtt ttg ccg tcg cag ctg cgt gac atg gtt 883 Wing Phe Thr Wing Tyr Wing Val Leu Pro Ser Gln Leu Arg Asp Met Val 250 255 260 15 tct gca ccc gtt gcg tat tct gcg ctg atc gct ttg gtt acc tta ggt 931 Be Ala Pro Val Ala Tyr Be Ala Leu He Ala Leu Val Thr Leu Gly 265 270 275 tct gga ttt ggt atc caa caa ttc ggt ect caa atc atg ggc acc tct 979 Ser Gly Phe Gly He Gln Gln Phe Gly Pro Gln He Met Gly Thr Ser 280 285 290 aaa act cgc ggg ccg att ttg gcc atg ttc gtc here gtc atc ggc atg 1027 Lys Thr Arg Gly Pro He Leu Wing Met Phe Val Thr Val He Gly Met 295 300 305 20 ate ggc gcg gtg atc gtg atg aac ect cat cca tgg tgg gcg cta 1075 He Gly Ala Val He Val Val Met Asn Pro His Pro Trp Trp Ala Leu 310 315 320 325 gtt ggc tgc atg gcc ctc ggc ctg tct tat ggc ctg tgt atg ttc atg 1123 Val Gly Cys Met Ala Leu Gly Leu Ser Tyr Gly Leu Cys Met Phe Met 330 335 340 ggg ttg gcg gaa act aa att gct cca ect att gat atg gca ggc 1171 Gly Leu Wing Glu Thr Gln Asn He Wing Pro Pro He Asp Met Wing Gly 345 350 355 25 ctg acg ggt att ttc tac tgc ctg acg tac gta ggt atg gtc ttt cca 1219 -Late aaa-aMfaamwii-.

Leu Thr Gly He Phe Tyr Cys Leu Thr Tyr Val Gly Met Val Phe Pro 360 365 370 gcc ttg atg acc tgg ttg aat caa tgg ctc agt tac ccg ttc atg ctg 1267 Wing Leu Met Thr Trp Leu Asn Gln Trp Leu Ser Tyr Pro Phe Met Leu 375 380 385 ggc ttt ggt gcg gtg atg gca act att tgt ctg atc att gtg agt ttt 1315 Gly Phe Gly Ala Val Met Wing Thr He Cys Leu He He Val Phe 390 395 400 405 agt gca cgc cga ttc tgagaaacaa ctaaagtgag cca 1353 Ser Ala Arg Arg Phe 410 < 210 > 154 < 211 > 410 < 212 > PRT < 213 > Corynebacterium glutamicum 10 < 400 > 154 Met Gly Val Ser Wing Leu Asn Met Ser Asp Met Val Wing Asn Lys Arg 1 5 10 15 Wing Gln Arg Lys Val Trp Leu Wing Val Wing Leu Ser Val Phe Thr Val 20 25 30 Wing Trp Gly Gly Asn Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly 35 40 45 Glu Gly Phe Phe Ser Asn Leu Phe He Asp Leu Leu Leu Val Phe Tyr 15 50 55 60 Wing He Gly Val Wing Val Gly Leu Leu Wing Wing Gly Pro Leu Ser Asp 65 70 75 80 Arg Tyr Gly Arg Arg Wing Val Met Leu Pro Wing Pro Leu He Wing 85 90 95 • Leu Gly Be Ala Leu He Wing Ser Gly Glu Glu Thr Wing He Leu He 100 105 110 Wing He Gly Arg Val Leu Ser Gly He Ser Val Gly Met Val Met Thr 20 115 120 125 Wing Gly Gly Ser Trp He Lys Glu Leu Ser Ser Arg Phe Glu Pro 130 135 140 Gly Val Lys Thr Ser Ala Gly Ala Lys Arg Ala Ser Met Ser Leu Thr 145 150 155 160 Gly Gly Phe Wing Leu Gly Pro Wing Leu Wing Gly Val Met Wing Gln Trp 165 170 175 Leu Pro Leu Pro Gly Gln Leu Wing Tyr Val Leu His He He Le Le Thr 25 180 185 190 - - ^ '~ -JgÉm? R'? R? Fr? I? I »fc - i Leu He Leu Phe Pro Leu Leu He Thr Wing Pro Glu Thr Arg Gln Ser 195 200 205 Wing His Leu Lys Thr Lys Gly Ser Phe Trp Ser Asp Val Leu Val Pro 210 215 220 Be Ala Leu Asp Lys Arg Phe Leu Phe Val Val Ala Pro He Gly Pro 225 230 235 240 Trp Val Phe Gly Wing Wing Phe Thr Wing Tyr Wing Val Leu Pro Ser Gln 245 250 255 Leu Arg Asp Met Val Ser Wing Pro Val Wing Tyr Being Wing Leu He Wing 260 265 270 Leu Val Thr Leu Gly Ser Gly Phe Gly He Gln Gln Phe Gly Pro Gln 275 280 285 He Met Gly Thr Ser Lys Thr Arg Gly Pro He Leu Wing Met Phe Val 290 295 300 Thr Val He Gly Met He Gly Wing Val He Val Val Met Asn Pro His 305 310 315 320 Pro Trp Trp Wing Leu Val Gly Cys Met Wing Leu Gly Leu Ser Tyr Gly 325 330 335 Leu Cys Met Phe Met Gly Leu Wing Glu Thr Gln Asn He Wing Pro Pro 340 345 350 He Asp Met Wing Gly Leu Thr Gly He Phe Tyr Cys Leu Thr Tyr Val 355 360 365 Gly Met Val Phe Pro Wing Leu Met Thr Trp Leu Asn Gln Trp Leu Ser 370 375 380 Tyr Pro Phe Met Leu Gly Phe Gly Wing Val Met Wing Thr He Cys Leu 385 390 395 400 He He Val Ser Phe Ser Ala Arg Arg Phe 405 410 < 210 > 155 < 211 > 703 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (703) < 223 > FRXA00803 < 400 > 155 tcatccttcc ttagctcgcg tgagcttccc aagcgtaagc acccccgtgt gagggcataa 60 cggccgttct gttaaagatt ggtctggcca tttcctccat atg ggg gtg tcc gcg 115 Met Gly Val Ser Wing 1 5 ctt aac atg tct gac atg gtg gcg aac aaa cgg gca cag cgt aaa gtc 163 Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg Wing Gln Arg Lys Val 10 15 20 tgg cta gcg gta gct tta tcg gtc ttt acg gtc gcg tgg ggt ggc aat 211 Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val Wing Trp Gly Gly Asn 25 30 35 gaa ttc act ccc ttg ctg gtg ttt tac cg ggt gag ggg ttc ttt age 259 Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly Glu Gly Phe Phe Ser 40 45 50 aac ctg ctg atc gac ctt ttg ctg gtg gtg ttt tat gcc atc gga gta gcg 307 Asn Leu Phe He Asp Leu Leu Leu Val Phe Tyr Wing He Gly Val Wing 55 60 65 gta ggt ttg ctg gca gct gcttctct ttacct gaccgc tat ggc cgacgt 355 Val Gly Leu Leu Wing Wing Gly Pro Leu Ser Asp Arg Tyr Gly Arg Arg 70 75 80 85 gcc gtc atg ttg ect gcg cca ttg atc gcg atc ttg ggt tcc gcg ttg 403 Wing Val Met Leu Pro Wing Pro Leu He Wing He Leu Gly Wing Wing Leu 90 95 100 att gcc tcg ggt gaa gaa acc gcc atc ctg att gcc att ggt cga gtg 451 He Wing Ser Gly Glu Glu Thr Wing He Leu He Wing He Gly Arg Val 105 110 115 ctg tcg gga att tcg gtg ggc atg gtg atg here gcg gga ggt tcc tgg 499 Leu Ser Gly He Ser Val Gly Met Val Met Thr Wing Gly Gly Ser Trp 120 125 130 att aag gag ctt tea tcg tcg cgg ttt gag cca ggg gtg aaa acc agt 547 He Lys Glu Leu Being Ser Arg Phe Glu Pro Gly Val Lys Thr Ser 135 140 145 ggt ggt gca aaa cgc gca tcg atg tct ttg gt gt gt gt gt gtc ctc 595 Wing Gly Ala Lys Arg Ala Ser Met Ser Leu Thr Gly Gly Phe Ala Leu 150 155 160 165 ggc cea gcg ctt gct ggt atg gg cag tgg ctg cca caa ect gga 643 Gly Pro Ala Leu Ala Gly Val Met Wing Gln Trp Leu Pro Gln Pro Gly 170 175 180 cag ttg gca tat gtt ttg cac att att ctc act ctg att ttg ttc ccg 691 Gln Leu Ala Tyr Val Leu His He He Leu Thr Leu He Leu Phe Pro 185 190 195 ttg ctt att here 703 Leu Leu He Thr 200 <; 210 > 156 < 211 > 201 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 156 Met Gly Val Ser Ala Leu Asn Met Ser Asp Met Val Ala Asn Lys Arg 1 5 10 15 Ala Gln Arg Lys Val Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val 20 25 30 Wing Trp Gly Gly Asn Glu Phe Thr Pro Leu Leu Val Phe Tyr Arg Gly 35 40 45 Glu Gly Phe Phe Ser Asn Leu Phe He Asp Leu Leu Leu Val Phe Tyr 50 55 60 Wing He Gly Val Wing Val Gly Leu Leu Wing Wing Gly Pro Leu Ser Asp 65 70 75 80 Arg Tyr Gly Arg Arg Wing Val Met Leu Pro Wing Pro Leu He Wing He 85 90 95 Leu Gly Be Ala Leu He Wing Be Gly Glu Glu Thr Wing He Leu He 100 105 110 Wing He Gly Arg Val Leu Ser Gly He Ser Val Gly Met Val Met Thr 115 120 125 Wing Gly Gly Ser Trp He Lys Glu Leu Ser Ser Arg Phe Glu Pro 130 135 140 Gly Val Lys Thr Ser Wing Gly Wing Lys Arg Wing Ser Met Ser Leu Thr 145 150 155 160 Gly Gly Phe Wing Leu Gly Pro Wing Leu Wing Gly Val Met Wing Gln Trp 165 170 175 Leu Pro Gln Pro Gly Gln Leu Wing Tyr Val Leu His He He Leu Thr 180 185 190 Leu He Leu Phe Pro Leu Leu He Thr 195 200 < 210 > 157 < 211 > 1014 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (991) < 223 > RXA01407 < 400 > 157 atccggggaa cggatcccaa agatctcctt gatgccatcg cgtttttaac ctggccagct 60 ctggttgccc cagtgatcgc cccacttctg ggaggtcttc ttg caa gat acc att 115 Leu Gln Asp Thr He 1 5 ggt tgc cga tgg atc ttc ctc ctc aac gtg ccc tta gga atc atc gcg 163 Gly Cys Arg Trp He Phe Leu Leu Asn Val Pro Leu Gly He He Wing 10 15 20 atc atg gct gga cta ttc atc cag ccc aag aac acg gcc gtg aat gtg 211 He Met Wing Gly Leu Phe He Gln Pro Lys Asn Thr Wing Val Asn Val 25 30 35 aag cga ttt gat cgg cca ggt ttc ctc ggc gca atg ctg gtg atg gtg 259 Lys Arg Phe Asp Arg Pro Gly Phe Leu Gly Wing Met Leu Val Met Val 40 45 50 gcg caa gcc gtg att gcg gag tta att tgc age aga agt ccg gcc gca 307 Wing Gln Wing Wing Val He Wing Glu Leu He Cys Ser Arg Ser Pro Wing Wing 55 60 65 ctt act atc tgt gca tgc ctc gtc tta agt gct gcg gtg gta tgc ggt 355 Leu Thr He Cys Wing Cys Leu Val Leu Ser Wing Wing Val Val Cys Gly 70 75 80 85 ttt gta gtg cgc tgg ctg cga gtt cca ggc cga ctt ttt gat ctc age 403 Phe Val Val Arg Trp Leu Arg Val Pro Gly Arg Leu Phe Asp Leu Ser 90 95 100 atc atc cgc atc cca ggt ttc cga gtg gg aat tcc tcc gga agt atc 451 He Met Arg He Pro Gly Phe Arg Val Gly Asn Ser Ser Gly Be He 105 110 115 tac cgc ttg gta atc acc gca gca cca ttc atg ttc act ttg ctc ttc 499 Tyr Arg Leu Val He Thr Wing Wing Pro Phe Met Phe Thr Leu Leu Phe 120 125 130 caa gtg gcg ttt ggg tgg tct gca here tta gcg ggt gcc atg gtg gtc 547 Gln Val Wing Phe Gly Trp Ser Wing Thr Leu Wing Gly Wing Met Val Val 135 140 145 gca cta tcc gca ggc aac gtg gca atc aaa ect tcc acc acc ccg atc 595 Wing Leu Phe Wing Gly Asn Val Wing He Lys Pro Phe Thr Thr Pro He 150 155 160 165 att aaa cgc tgg aat ttc aaa cca gta ctg gtc ttt tct aac gct gct gct 643 He Lys Arg Trp Asn Phe Lys Pro Val Leu Val Phe Ser Asn Wing Wing 170 175 180 ggc gcc ttg gta ttg gca act ttt ttg ttc gtt cgt gca gat acc cca 691 Gly Ala Leu Val Leu Wing Thr Phe Leu Phe Val Arg Wing Asp Thr Pro 185 190 195 ctg gtt ctc atc gtg ctg ctg ctc ttt gtt tcg ggc gca tta agg tcc 739 Leu Val Leu He Val Leu Leu Leu Phe Val Ser Gly Ala Leu Arg Ser 200 205 210 ctc ggt ttc age gcc tac aac acc ttg cag ttt gtc gat atc tea cca 787 Leu Gly Phe Ser Wing Tyr Asn Thr Leu Gln Phe Val Asp He Ser Pro 215 220 225 gaa caa acc age aac gcc aac gtg tta tea gca acc ctg cac caa cta 835 Glu Gln Thr Ser Asn Wing Asn Val Leu Ser Wing Thr Leu His Gln Leu 230 235 240 245 ggc atg tct ttg ggt att gcg gta gca gtc atc gcc atg tcc ctt gca 883 Gly Met Ser Leu Gly He Ala Val Val Ala He Ala Met Ser Leu Ala 250 255 260 ccc acc gcc aac tgg gca ttc cca cca gca gcg ttg ttc ctc att 931 Pro Thr Wing Asn Trp Wing Phe Pro Leu Wing Wing Wing Leu Phe Leu He 265 270 275 ect cta atc ggc gca cta tct tct ect cgc gac ggc ggt gcc cga gcc 979 Pro Leu He Gly Ala Leu Ser Leu Pro Arg Asp Gly Gly Ala Arg Ala 280 285 290 ttt tcc tct tct tagaaaecca cttctgaaag gta 1014 Phe Ser Ser Ser 295 < 210 > 158 < 211 > 297 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 158 Leu Gln Asp Thr He Gly Cys Arg Trp He Phe Leu Leu Asn Val Pro 1 5 10 15 Leu Gly He He Wing He Met Wing Gly Leu Phe He Gln Pro Lys Asn 20 25 30 Thr Wing Val Asn Val Lys Arg Phe Asp Arg Pro Gly Phe Leu Gly Ala 35 40 45 Met Leu Val Met Val Ala Gln Ala Val He Ala Glu Leu He Cys Ser 50 55 60 Arg Ser Pro Ala Ala Leu Thr He Cys Ala Cys Leu Val Leu Ser Ala 65 70 75 80 Wing Val Val Cys Gly Phe Val Val Arg Trp Leu Arg Val Pro Gly Arg 85 90 95 Leu Phe Asp Leu Ser He Met Arg He Pro Gly Phe Arg Val Gly Asn 100 105 110 Ser Ser Gly Ser He Tyr Arg Leu Val He Thr Ala Wing Pro Phe Met 115 120 125 Phe Thr Leu Leu Phe Gln Val Wing Phe Gly Trp Ser Wing Thr Leu Wing 130 135 140 Gly Wing Met Val Val Wing Ala Leu Phe Wing Gly Asn Val Wing He Lys Pro 145 150 155 160 Phe Thr Thr Pro He He Lys Arg Trp Asn Phe Lys Pro Val Leu Val 165 170 175 Phe Ser Asn Wing Wing Gly Wing Leu Val Leu Wing Thr Phe Leu Phe Val 180 185 190 Arg Wing Asp Thr Pro Leu Val Leu He Val Leu Leu Leu Phe Val Ser 195 200 205 Gly Ala Leu Arg Ser Leu Gly Phe Ser Ala Tyr Asn Thr Leu Gln Phe 210 215 220 Val Asp He Ser Pro Glu Gln Thr Ser Asn Ala Asn Val Leu Ser Ala 225 230 235 240 Thr Leu His Gln Leu Gly Met Be Leu Gly He Wing Val Wing Val He 245 250 255 Wing Met Ser Leu Wing Pro Thr Wing Asn Trp Wing Phe Pro Leu Wing Wing 260 265 270 Wing Leu Phe Leu He Pro Leu He Gly Wing Leu Ser Leu Pro Arg Asp 275 280 r 285 Gly Gly Ala Arg Ala Phe Ser Ser Ser 290 295 < 210 > 159 < 211 > 324 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (301) < 223 > RXA01408 < 400 > 159 cgcatagagt tatctcgaaa ctaccaagac ggtttctttt ctttagatct aaggaggaga 60 gaccgtcttt caccctttca tctgattgga catcgacgcc] atg cgc aat gat cgg 115 Met Arg Asn Asp Arg 1 5 tcc ttt age gtt ccc att gcg cta ctt gcc gcg gga gca ctg ttt cta 163 Ser Phe Ser Val Pro He Ala Leu Leu Ala Ala Gly Ala Leu Phe Leu 10 15 20 gaa atc ctc gac ggc acc atc ctg here ac gca gtg cca gct att gct 211 Glu He Leu Asp Gly Thr He Leu Thr Thr Ala Val Pro Ala He Wing 25 30 35 cgt gac ttc ggt att gac gcc gtg gat gtc age att gca ctg gtt gct 259 Arg Asp Phe Gly He Asp Ala Val Asp Val Ser He Ala Leu Val Ala 40 45 50 tac ttg gca gcc gca gca gct ggc att ccg ctg cag ggt ggc 301 Tyr Leu Ala Ala Ala Ala Ala Gly He Pro Leu Gln Gly Gly 55 60 65 tagcggatcg atttggtgtg cgc 324 < 210 > 160 < 211 > 67 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 160 Met Arg Asn Asp Arg Ser Phe Ser Val Pro He Wing Leu Leu Wing Wing 1 5 10 15 Gly Wing Leu Phe Leu Glu He Leu Asp Gly Thr He Leu Thr Thr Wing 20 25 30 Val Pro Wing He Wing Arg Asp Phe Gly He Asp Ala Val Asp Val Ser 35 40 45 He Ala Leu Val Ala Tyr Leu Ala Ala Ala Ala Ala Ala Gly He Pro Leu 50 55 60 Gln Gly Gly 65 < 210 > 161 < 211 > 1275 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1252) < 223 > RXN01922 < 400 > 161 acactccttt ggtcacctgg tttggttgag ggaaacagac cgcccaagaa cccaagaaat 60 cccaagaaaa catgctgctt atgaattaaa gtgagcaccc atg aga tea gga aac 115 Met Arg Ser Gly Asn 1 5 gcc aat cgc gtc ttc ata ggt gtt acc atc ctg ctg ttt act gca gga 163 Wing Asn Arg Val Phe He Gly Val Thr He Leu Leu Phe Thr Wing Gly 10 15 20 tgg gca gcc aat cat ttc gcg tea gtg ttg gtg ttg atc cgt gaa caa 211 Trp Ala Ala Asn His Phe Ala Ser Val Leu Val Leu He Arg Glu Gln 25 30 35 tta gac gta tea age gtg ctg gtc aac ggc gct ttt ggt att tat gca 259 Leu Asp Val Ser Ser Val Leu Val Asn Gly Wing Phe Gly He Tyr Wing 40 45 50 ctg gga ctt ctt cca agt ttg ctc gca ggc ggt gtg ctt gcc gac cgt 307 Leu Gly Leu Leu Pro Be Leu Leu Wing Gly Gly Val Leu Wing Asp Arg 55 60 65 ttt ggt gcc cgc atg gtg gta ctc acc gga ggt gta ctt tct gcg ctt 355 Phe Gly Ala Arg Met Val Val Leu Thr Gly Gly Val Leu Ser Ala Leu 70 75 80 85 gga aac ctt tct ctt tta gcg ttt cat gat ggt ect tcc ctc ctg gta 403 Gly Asn Leu Ser Leu Leu Ala Phe His Asp Gly Pro Ser Leu Leu Val 90 95 100 gga cga ttc atc gtt ggt ctg ggc gtt gga tta gtc gtc age gcg ggc 451 Gly Arg Phe He Val Gly Leu Gly Val Gly Leu Val Val Ser Ala Gly 105 110 115 action ggg tgc ggc ggc aga tgc ggg gca ggc gtg gtg here ttg gcc 499 Thr Wing Trp Wing Gly Arg Leu Arg Gly Wing Ser Gly Val Thr Leu Wing 120 125 130 ggc att att ctg acc gcc ggt ttc atg atg ggg ccg att gtg here agt 547 Gly He He Leu Thr Wing Gly Phe Met Met Gly Pro He Val Thr Ser 135 140 145 ggg ttg ggg atg gcg tcg here age att att acg ccc ttt gcc ata age 595 Gly Leu Gly Met Wing Ser Thr Ser He He Thr Pro Phe Wing He Ser 150 155 160 165 gtt gcc ctc tcg ctg atc gcg gtg gtt gtg gtg gtg gtt ctt ggc gat 643 Val Ala Leu Being Leu He Wing Val Val Gly Phe Wing Leu Gly Asp 170 175 180 gcc cgc age acc ccg age gca ctt ggc gca gcc tcc age gga atc aaa cac 691 Ala Arg Being Thr Pro Being Wing Leu Gly Wing Being Ser Gly He Lys His 185 190 195 gaa cga age atg aaa aag gcc ctc gcg gtg tcc ttg cg atg gca att 739 Glu Arg Ser Met Lys Lys Ala Leu Wing Val Ser Leu Pro Met Wing He 200 205 210 tgg gtg ttc age tgc atc acc tcc ctg atc gtg atg tcc gcg cgc 787 Trp Val Phe Ser Cys He Thr Thr Ser Leu He Val Met Ser Wing Arg 215 220 225 atc gac tcc acc ttc ggc aac gcc att ctt ctc ccc gga atc ggc gcg 835 He Asp Being Thr Phe Gly Asn Wing He Leu Leu Pro Gly He Gly Wing 230 235 240 245 gcg atc gcc ttc age gca ggc ctg atc gca caa ttt tta ggt agg aaa 883 Wing He Wing Phe Being Wing Gly Leu He Al a Gln Phe Leu Gly Arg Lys 250 255 260 ttc gcg tgg ggt cgt ggc tcc gga atc gtg ggc gcg ctg tgt gcc ctc 931 Phe Wing Trp Gly Arg Gly Ser Gly He Val Gly Wing Leu Cys Ala Leu 265 270 275 gcg ggt ttt gcg ctg gca gct ttt ggt ggc gac tcc att cca gtg tgg 979 Wing Gly Phe Wing Leu Wing Wing Phe Gly Gly Asp Ser He Pro Val Trp 280 285 290 ctt ttc gtt atc gcc tcg atc ctg ttc ggc acc gca tat ggc ctc tgc 1027 Leu Phe Val He Wing Be He Leu Phe Gly Thr Wing Tyr Gly Leu Cys 295 300 305 ctc cgc gaa ggc ctc ctc age atc gaa act tac acg cca ctc aac cga 1075 Leu Arg Glu Gly Leu Leu Ser He Glu Thr Tyr Thr Pro Leu Asn Arg 310 315 320 325 cgt ggc acc ggc atc ggc atc tat tat gtg ttc acg tat ttg gga ttc 1123 Arg Gly Thr Gly He Gly He Tyr Tyr Val Phe Thr Tyr Leu Gly Phe 330 335 340 ggg ctg cca gtg ctt ctc gac gcc ccc ctc ccg cac ctt ggc gcc tcc 1171 Gly Leu Pro Val Leu Leu Asp Ala Leu Leu Pro His Leu Gly Wing Ser 345 350 355 att ccg ctg tac gcg ctg gcg gcg ctc gcc ctt ggc tcc gca gta atc 1219 He Pro Leu Tyr Ala Leu Ala Ala Leu Ala Leu Gly Ser Ala Val He 360 365 370 cgc ggc gta caa atc aag cgc ggg tat gtg gtt tagatttcta cctacgacct 1272 Arg Gly Val Gln He Lys Arg Gly Tyr Val Val 375 380 gaa 1275 < 210 > 162 < 211 > 384 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 162 Met Arg Ser Gly Asn Wing Asn Arg Val Phe He Gly Val Thr He Leu 1 5 10 15 Leu Phe Thr Wing Gly Trp Wing Wing Asn His Phe Wing Being Val Leu Val 20 25 30 Leu He Arg Glu Gln Leu Asp Val Ser Val Leu Val Asn Gly Wing 35 40 45 Phe Gly He Tyr Ala Leu Gly Leu Leu Pro Ser Leu Leu Wing Gly Gly 50 55 60 Val Leu Wing Asp Arg Phe Gly Wing Arg Met Val Val Leu Thr Gly Gly 65 70 75 80 Val Leu Be Wing Leu Gly Asn Leu Be Leu Leu Wing Phe His Asp Gly 85 90 95 Pro Ser Leu Leu Val Gly Arg Phe He Val Gly Leu Gly Val Gly Leu 100 105 110 Val Val Ser Ala Gly Thr Ala Trp Ala Gly Arg Leu Arg Gly Ala Ser 115 120 125 Gly Val Thr Leu Ala Gly He He Leu Thr Ala Gly Phe Met Met Gly 130 135 140 Pro He Val Thr Ser Gly Leu Gly Met Wing Ser Thr Ser He He Thr 145 150 155 160 Pro Phe Ala He Ser Val Val Ala Leu Ser Leu He Ala Val Val Val Gly 165 170 175 Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Be Ala Leu Gly Ala Ser 180 185 190 Ser Gly He Lys His Glu Arg Ser Met Lys Lys Ala Leu Ala Val Ser 195 200 205 Leu Pro Met Ala He Trp Val Phe Ser Cys He Thr Thr Ser Leu He 210 215 220 Val Met Being Wing Arg He Asp Being Thr Phe Gly Asn Wing He Leu Leu 225 230 235 240 Pro Gly He Gly Wing Wing He Wing Phe Be Wing Gly Leu He Wing Gln 245 250 255 Phe Leu Gly Arg Lys Phe Wing Trp Gly Arg Gly Ser Gly He Val Gly 260 265 270 Wing Leu Cys Wing Leu Wing Gly Phe Wing Leu Wing Wing Phe Gly Gly Asp 275 280 285 Ser He Pro Val Trp Leu Phe Val He Wing Be Ser Leu Phe Gly Thr 290 295 300 Wing Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser He Glu Thr Tyr 305 310 315 320 Thr Pro Leu Asn Arg Arg Gly Thr Gly He Gly He Tyr Tyr Val Phe 325 330 335 Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Wing Leu Leu Pro 340 345 350 His Leu Gly Wing Being Pro Pro Leu Tyr Wing Leu Wing Wing Leu Wing Leu 355 360 365 Gly Being Wing Val Hearing Arg Gly Val Gln He Lys Arg Gly Tyr Val Val 370 375 380 < 210 > 163 < 211 > 1130 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (1107) < 223 > FRXA01922 < 400 > 163 ctg ctg ttt act gca gga tgg gca gcc aat cat ttc gcg tea gtg ttg 48 Leu Leu Phe Thr Wing Gly Trp Wing Wing Asn His Phe Wing Being Val Leu 1 5 10 15 gtg ttg atc cgt gaa caa tta gac gta tea age gtg ctg gtc aac ggc 96 Val Leu He Arg Glu Gln Leu Asp Val Ser Ser Val Leu Val Asn Gly 20 25 30 gct ttt ggt att tat gca ctg gga ctt ctt cca agt ttg ctc gca ggc 144 Ala Phe Gly He Tyr Ala Leu Gly Leu Leu Pro Ser Leu Leu Wing Gly 35 40 45 ggt gtg ctt gcc gac cgt ttt ggt gcc gg cgc atg gtg gta ctc acc gga 192 Gly Val Leu Wing Asp Arg Phe Gly Wing Arg Met Val Val Leu Thr Gly 50 55 60 ggt gta ctt tct gcg ctt gga aac ctt tct ctt tta gcg ttt cat gat 240 Gly Val Leu Ser Wing Leu Gly Asn Leu Ser Leu Leu Wing Phe His Asp 65 70 75 80 ggt ect tcc ctc ctg gta gga cga ttc atc gtt ggt ctg ggc gtt gga 288 Gly Pro Ser Leu Leu Val Gly Arg Phe He Val Gly Leu Gly Val Gly 85 90 95 tta gtc gtc age gcg ggc acc gca tgg gcg ggc aga ttg cgc gga gca 336 Leu Val Val Ser Wing Gly Thr Wing Trp Wing Gly Arg Leu Arg Gly Ala 100 105 110 age ggc gtg here ttg gcc ggc att att ctg acc gcc gt ttc atg atg 384 Ser Gly Val Thr Leu Wing Gly He He Leu Thr Wing Gly Phe Met Met 115 120 125 999 GC9 att 9t aca a9 ^ 999 tt: 999 at9 9C9 tc9 here a9c att att ^ 32 Gly Pro He Val Thr Ser Gly Leu Gly Met Wing Ser Thr Ser He He 130 135 140 acg ccc ttt gcc ata age gtt gcc ctc tcg ctg atc gcg gtg gtt gtg 480 Thr Pro Phe Ala He Ser Val Ala Leu Ser Leu He Wing Val Val Val 145 150 155 160 gga ttt gcg ctt ggc gat gcc cgc age acc ccg age gca ctt ggc gca 528 Gly Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala 165 170 175 tcc age gga atc aaa cac gaa cga age atg aaa aag gcc ctc gcg gtg 576 jffátoíj Ser Ser Gly He Lys His Glu Arg Ser Met Lys Lys Wing Leu Wing Val 180 185 190 tcc ttg ccg atg gca att tgg gtg ttc age tgc atc acc acc tcc ctg 624 Ser Leu Pro Met Wing He Trp Val Phe Ser Cys He Thr Thr Ser Leu 195 200 205 atc gtg atg tcc gcg cgc atc gac tcc acc ttc ggc aac gcc att ctt 672 5 He Val Met Ser Wing Arg He Asp Ser Thr Phe Gly Asn Wing He Leu • 210 215 220 ctc ccc gga atc ggc gcg gcg atc gcc ttc age gca ggc ctg atc gca 720 Leu Pro Gly He Gly Wing Wing Wing Phe Wing Wing Gly Leu Wing 225 230 235 240 caa ttt tta ggt agg aaa ttc gcg tgg ggt cgt ggc tcc gga atc gtg 768 Gln Phe Leu Gly Arg Lys Phe Wing Trp Gly Arg Gly Ser Gly He Val 245 250 255 ggc gcg ctg tgt gcc ctc gcg ggt ttt gcg ctg gca gct ttt ggt ggc 816 10 Gly Ala Leu Cys Ala Leu Ala Gly Phe Ala Leu Ala Ala Phe Gly Gly 260 265 270 gac tcc att cca gtg tgg ctt ttc gtt atc gcc tcg atc ctg ttc ggc 864 Asp Ser He Pro Val Trp Leu Phe Val He Wing Ser He Leu Phe Gly 275 280 285 acc gca tat ggc ctc tgc ctg cgc gaa ggc ctc ctc age atc gaa act 912 Thr Ala Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser He Glu Thr 290 295 300 tac acg cca ctc aac cga cgt ggc acc ggc atc ggc atc tat tat gtg 960 15 Tyr Thr Pro Leu Asn Arg Arg Gly Thr Gly He Gly He Tyr Tyr V al 305 310 315 320 ttc acg tat ttg gga ttc ggg ctg cca gtg ctt ctc gac gcc ctc ctc 1008 Phe Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Ala Leu Leu 325 330 335 ccg cac ctt ggc gcc tcc att ccg ctg tac gcg ctg gcg gcg ctc gcc 1056 Pro His Leu Gly Ala Ser He Pro Leu Tyr Ala Leu Ala Ala Leu Wing 340 345 350 ctt ggc tcc gca gta atc cgc ggc gta caa atc aag cgc ggg tat gtg 1104 20 Leu Gly Ser Ala Val He Arg Gly Val Gln He Lys Arg Gly Tyr Val 355 360 365 gtt tagatttcta cctacgacct gaa 1130 Val < 210 > 164 < 211 > 369 < 212 > PRT < 213 > Corynebacterium glutamicum 25 .sS? ms? lfa & m ?. ? & > a # í * < 400 > 164 Leu Leu Phe Thr Ala Gly Trp Ala Ala Asn His Phe Ala Ser Val Leu 1 5 10 15 Val Leu He Arg Glu Gln Leu Asp Val Ser Ser Val Leu Val Asn Gly 20 25 30 Wing Phe Gly He Tyr Wing Leu Gly Leu Leu Pro Ser Leu Leu Wing Gly 35 40 45 • Gly Val Leu Wing Asp Arg Phe Gly Wing Arg Met Val Val Leu Thr Gly 50 55 60 Gly Val Leu Ser Wing Leu Gly Asn Leu Ser Leu Leu Wing Phe His Asp 65 70 75 80 Gly Pro Ser Leu Leu Val Gly Arg Phe He Val Gly Leu Gly Val Gly 85 90 95 Leu Val Val Ser Wing Gly Thr Wing Trp Wing Gly Arg Leu Arg Gly Wing 10 100 105 110 Ser Gly Val Thr Leu Wing Gly He He Leu Thr Wing Gly Phe Met Met 115 120 125 Gly Pro He Val Thr Ser Gly Leu Gly Met Wing Ser Thr Ser He He 130 135 140 Thr Pro Phe Wing He Ser Val Wing Leu Ser Leu He Ala Val Val Val 145 150 155 160 Gly Phe Ala Leu Gly Asp Ala Arg Ser Thr Pro Ser Ala Leu Gly Ala 15 165 170 175 Ser Ser Gly He Lys His Glu Arg Ser Met Lys Lys Wing Leu Wing Val 180 185 190 Being Leu Pro Met Wing He Trp Val Phe Ser Cys He Thr Thr Ser Leu 195 200 205 He Val Met Ser Wing Arg He Asp Being Thr Phe Gly Asn Wing He Leu 210 215 220 Leu Pro Gly He Gly Wing Wing He Wing Phe Being Wing Gly Leu He Wing 20 225 230 235 240 Gln Phe Leu Gly Arg Lys Phe Wing Trp Gly Arg Gly Ser Gly He Val 245 250 255 Gly Wing Leu Cys Wing Leu Wing Gly Phe Wing Leu Wing Wing Phe Gly Gly 260 265 270 Asp Ser He Pro Val Trp Leu Phe Val He Wing He Leu Phe Gly 275 280 285 Thr Wing Tyr Gly Leu Cys Leu Arg Glu Gly Leu Leu Ser He Glu Thr 25 290 295 300 Tyr Thr Pro Leu Asn Arg Arg Gly Thr Gly He Gly He Tyr Tyr Val 305 310 315 320 Phe Thr Tyr Leu Gly Phe Gly Leu Pro Val Leu Leu Asp Wing Leu Leu 325 330 335 Pro His Leu Gly Wing Be He Pro Leu Tyr Wing Leu Wing Wing Leu Wing 340 345 350 Leu Gly Ser Wing Val He Arg Gly Val Gln He Lys Arg Gly Tyr Val 355 360 365 Val < 210 > 165 < 211 > 362 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (339) < 223 > RXA02060 < 400 > 165 gaa ttt gcc cgc att ttg aag cca aag gga cag gtc atc gtg ctt acc 48 Glu Phe Wing Arg He Leu Lys Pro Lys Gly Gln Val He Val Leu Thr 1 5 10 15 gca gat acc ggc cac ttg gct gag ctt cgt gaa cca ctg ggc atc att 96 Wing Asp Thr Gly His Leu Wing Glu Leu Arg Glu Pro Leu Gly He He 20 25 30 gat gtg gag gcc ggc aaa gtt gat cgc atg atc gaa caa gcg gca ggc 144 Asp Val Glu Wing Gly Lys Val Asp Arg Met He Glu Gln Ala Ala Gly 35 40 45 cac ctc aag cca gtt ggc gaa aga gac ttg gtg gaa ttt gaa atg ctg 192 His Leu Lys Pro Val Gly Glu Arg Asp Leu Val Glu Phe Glu Met Leu 50 55 60 ctg gat aaa tcc att gca tct cag atc ggt atg age ect tct gca 240 Leu Asp Gln Lys Ser He Wing Ser Gln He Gly Met Ser Ser Pro Wing 65 70 75 80 cgc cac att aag ect gag gct ttg gcg gaa cgc atc gcc gct cta cca 288 Arg His He Lys Pro Glu Ala Leu Wing Glu Arg He Wing Wing Leu Pro 85 90 95 gaa caa atg aag gtt aca gcc cgg gcc aag atc acc agg ctg gaa cgc 336 Glu Gln Met Lys Val Thr Ala Arg Ala Lys He Thr Arg Leu Glu Arg 100 105 110 atc taact ettat ctcactgggc ctt 362 He < 210 > 166 < 211 > 113 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 166 Glu Phe Wing Arg He Leu Lys Pro Lys Gly Gln Val He Val Leu Thr 1 5 10 15 Wing Asp Thr Gly His Leu Wing Glu Leu Arg Glu Pro Leu Gly He He 20 25 30 Asp Val Glu Wing Gly Lys Val Asp Arg Met He Glu Gln Ala Wing Gly 35 40 45 His Leu Lys Pro Val Gly Glu Arg Asp Leu Val Glu Phe Glu Met Leu 50 55 60 Leu Asp Gln Lys Ser He Wing Ser Gln He Gly Met Ser Ser Pro Wing 65 70 75 80 Arg His He Lys Pro Glu Ala Leu Wing Glu Arg He Wing Wing Leu Pro 85 90 95 Glu Gln Met Lys Val Thr Wing Arg Wing Lys He Thr Arg Leu Glu Arg 100 105 110 He < 210 > 167 < 211 > 1395 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1372) < 223 > RXN01936 < 400 > 167 gcgcggtgac accacagccg ttgtcagcgg cgcttggtct gtggaggatc gccgaggtta 60 ctaacaaata ggcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115 Val Ser Phe Arg Asp 1 5 att ttc gct gac acc aga ccg ctg aaa gaa ccg gcc ttc aaa cgc ctc 163 He Phe Wing Asp Thr Arg Pro Leu Lys Glu Pro Wing Phe Lys Arg Leu 10 15 20 tgg ctt ggc aat gtt gcc acc gtc att ggt gcc caa tta act gtt gtt 211 Trp Leu Gly Asn Val Wing Thr Val He Gly Wing Gln Leu Thr Val Val 25 30 35 gcc gtt ccg gtg cag att tac caa atg act ggg tcc tcc ggc tat gtg 259 Wing Val Pro Val Gln He Tyr Gln Met Thr Gly Ser Ser Gly Tyr Val 40 45 50 ggc ttg acc ggg ctt ttt ggc ctt att ect ttg gtt att ttt ggc ctt 307 Gly Leu Thr Gly Leu Phe Gly Leu He Pro Leu Val He Phe Gly Leu 55 60 65 tat ggt gga tcc att gcg gat gct ttt gat aaa cgc atc gtg ctg atc 355 Tyr Gly Gly Ser He Wing Asp Wing Phe Asp Lys Arg He Val Leu He 70 75 80 85 tgc acc acg atc ggc atg tgt gtc acc act gcc ttt tgg gtg ctg 403 Cys Thr Thr He Gly Met Cys Val Thr Thr Ala Gly Phe Trp Val Leu 90 95 100 acc att tta ggc aat gag aat att tgg ctc ctg tta ata aac ttt tct 451 Thr He Leu Gly Asn Glu Asn He Trp Leu Leu Leu He Asn Phe Ser 105 110 115 tta cag gca ttt ttc gcg gtg aat caa ccc acc cga acg gcg atc 499 Leu Gln Gln Wing Phe Phe Wing Val Asn Gln Pro Thr Arg Thr Wing He 120 125 130 ctt cga agt att ttg ccg att gat caa tta gcg tcg gca aca tea ctg 547 Leu Arg Ser He Leu Pro He Asp Gln Leu Wing Ser Wing Thr Ser Leu 135 140 145 aat atg ctg ctc atg cag acc ggc gc atc gtt ggc ccg ctg atc gca 595 Asn Met Leu Leu Met Gln Thr Gly Ala He Val Gly Pro Leu He Ala 150 155 160 165 ggt gcg ttg att ccg ctg atc ggt ttc ggg tgg ctg tat ttc ctt gat 643 Gly Ala Leu He Pro Leu He Gly Phe Gly Trp Leu Tyr Phe Leu Asp 170 175 180 gtt gtc tcc atc atc ccc aca ctg tgg gct gta tgg tea ctg ect tcg 691 Val Val Ser He He Pro Thr Leu Trp Wing Val Trp Ser Leu Pro Ser 185 190 195 atc aag cca tcc ggc aag gtg atg aag gct ggt ttc gcc agt gtg gtg 739 He Lys Pro Ser Gly Lys Val Met Lys Wing Gly Phe Wing Ser Val Val 200 205 210 gat ggc ctg aag tat ttg gct ggc caa ccc gtg ttg ttg atg gtg atg 787 Asp Gly Leu Lys Tyr Leu Wing Gly Gln Pro Val Leu Leu Met Val Met 215 220 225 gtg ctg gat ctt atc gcc atg att ttc ggc atg cca cgt gcg ctt tac 835 Val Leu Asp Leu He Wing Met He Phe Gly Met Pro Arg Wing Leu Tyr 230 235 240 245 ccc gag atc gca gaa gtg aac ttc ggt ggg ggt gac gcc ggt gca acg 883 Pro Glu He Wing Glu Val Asn Phe Gly Gly Gly Asp Wing Gly Wing Thr 250 255 260 atg ctg gcg ttc atg tac tea tcc atg gct gtt ggc gca gtt ctt ggc 931 Met Leu Wing Phe Met Tyr Ser Ser Wing Wing Val Gly Wing Val Leu Gly 265 270 275 ggc gtg ctg tct ggt tgg gtg gcc cgg att age cgc cag ggt gtt gca 979 Gly Val Leu Ser Gly Trp Val Wing Arg He Ser Arg Gln Gly Val Wing 280 285 290 • gtt tat tgg tgc atc atc gcc tgg ggc gca gcc gtt gct tgg ggt ggt 1027 Val Tyr Trp Cys He He Wing Trp Gly Wing Wing Val Wing Leu Gly Gly 295 300 305 gtg gca att gtt gtc age ccc ggc gcg gtg act gcg tgg gcg tgg atg 1075 Val Ala He Val Val Ser Pro Gly Ala Val Thr Ala Trp Ala Trp Met 310 315 32 0 325 ttc atc atc atg atg att gtc att ggt atg gct gac atg ttc age tcg 1123 phe he met Met Met Val He Gly Met Met Wing Asp Met Phe Ser Ser 10 330 335 340 gca gtt cga aac gct att ttg cag cag tct gct gcg gaa cat gtg cag 1171 Wing Val Arg Asn Wing He Leu Gln Gln Wing Wing Wing Glu His Val Gln 345 350 355 ggc cga atc caa ggt gtg tgg atc atc gtc gtg gtg ggt gga ect cgt 1219 Gly Arg He Gln Gly Val Trp He He Val Val Val Gly Gly Pro Arg 360 365 370 tta gct gac gtc ctt cac ggt tgg gcc gct gact ccc ccc ggc gca ggt 1267 Leu Wing Asp Val Leu His Gly Trp Wing Wing Glu Pro Leu Gly Wing Gly 15 375 380 385 tgg acg gta tta tgg ggc gga gta gcg gtg gtt gta ctc act gca att 1315 Trp Thr Val Leu Trp Gly Val Val Val Val Val Leu Thr Ala He 390 395 400 405 tgt atg gtg gcg gtg ect aaa ttc tgg aaa tac gag aaa cca aaa att 1363 Cys Met Val Wing Val Pro Lys Phe Trp Lys Tyr Glu Lys Pro Lys He 410 415 420 acc ggc atc taaatactta tccatgccca ttt 1395 Thr Gly He 20 < 210 > 168 < 211 > 424 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 168 Val Ser Phe Arg Asp He Phe Wing Asp Thr Arg Pro Leu Lys Glu Pro 1 5 10 15 25 Wing Phe Lys Arg Leu Trp Leu Gly Asn Val Wing Thr Val He Gly Wing 20 25 30 Gln Leu Thr Val Val Wing Val Pro Val Gln He Tyr Gln Met Thr Gly 35 40 45 Ser Ser Gly Tyr Val Gly Leu Thr Gly Leu Phe Gly Leu He Pro Leu 50 55 60 5 Val He Phe Gly Leu Tyr Gly Gly Ser He Wing Asp Wing Phe Asp Lys 65 70 75 80 Arg He Val Leu He Cys Thr Thr He Gly Met Cys Val Thr Thr Wing 85 90 95 Gly Phe Trp Val Leu Thr He Leu Gly Asn Glu Asn He Trp Leu Leu 100 105 110 Leu He Asn Phe Ser Leu Gln Gln Wing Phe Phe Wing Val Asn Gln Pro 115 120 125 10 Thr Arg Thr Wing He Leu Arg Ser He Leu Pro He Asp Gln Leu Wing 130 135 140 Be Wing Thr Ser Leu Asn Met Leu Leu Met Gln Thr Gly Wing He Val 145 150 155 160 Gly Pro Leu He Ala Gly Ala Leu He Pro Leu He Gly Phe Gly Trp 165 170 175 Leu Tyr Phe Leu Asp Val Val Ser He He Pro Thr Leu Trp Wing Val 180 185 190 15 Trp Ser Leu Pro Ser He Lys Pro Ser Gly Lys Val Met Lys Wing Gly 195 200 205 Ph e Wing Val Val Asp Gly Leu Lys Tyr Leu Wing Gly Gln Pro Val 210 215 220 Leu Leu Met Val Met Val Leu Asp Leu He Wing Met He Phe Gly Met 225 230 235 240 Pro Arg Ala Leu Tyr Pro Glu He Ala Glu Val Asn Phe Gly Gly Gly 245 250 255 20 Asp Wing Gly Wing Thr Met Leu Wing Phe Met Tyr Ser Ser Wing Wing Val 260 265 270 Gly Wing Val Leu Gly Val Leu Ser Gly Trp Val Wing Arg He Ser 275 280 285 Arg Gln Gly Val Ala Val Tyr Trp Cys He He Wing Trp Gly Wing Wing 290 295 300 Val Wing Leu Gly Val Val Wing Val Val Ser Ser Gly Wing Val Thr 305 310 315 320 25 Wing Trp Wing Trp Met Phe He Met Met Met Val He Gly Met Gly Met 325 330 335 Asp Met Phe Ser Be Wing Val Arg Asn Wing He Leu Gln Gln Be Wing 340 345 350 Wing Glu His Val Gln Gly Arg He Gln Gly Val Trp He He Val Val 355 360 365 Val Gly Pro Arg Leu Ala Asp Val Leu His Gly Trp Wing Wing Glu 370 375 380 Pro Leu Gly Wing Gly Trp Thr Val Leu Trp Gly Val Val Val Wing Val 385 390 395 400 Val Leu Thr Wing He Cys Met Val Wing Val Pro Lys Phe Trp Lys Tyr 405 410 415 Glu Lys Pro Lys He Thr Gly He 420 < 210 > 169 < 211 > 945 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (922) < 223 > FRXA01936 < 400 > 169 tttacagcag gcatttttcg cggtgaatca acccacccga acggcgatcc ttcgaagtat 60 tttgccgatt gatcaataag cgtcggcaac atcactgaat atg ctg ctc atg cag 115 Met leu leu Met Gln 1 5 acc ggc gca atc gtt ggc ccg ctg atc gca ggt gcg ttg att ccg ctg 163 Thr Gly Ala He Val Gly Pro Leu He Wing Gly Wing Leu He Pro Leu 10 15 20 atc ggt ttc ggg tgg ctg tat ttc ctt gat gtt gtc tcc atc atc ccc 211 He Gly Phe Gly Trp Leu Tyr Phe Leu Asp Val Val Ser He He Pro 25 30 35 here ctg tgg gct gta tgg tea ctg ect tcg atc aag cca tcc ggc aag 259 Thr Leu Trp Wing Val Trp Ser Leu Pro Ser He Lys Pro Ser Gly Lys 40 45 50 gtg atg aag ggt ttc gcc agt gtg gtg gat ggc ctg aag tat ttg 307 Val Met Lys Wing Gly Phe Wing Ser Val Val Asp Gly Leu Lys Tyr Leu 55 60 65 gct ggc caa ccc gtg ttg ttg atg gtg atg gtg ctg gat ctt atc gcc 355 Wing Gly Gln Pro Val Leu Leu Met Val Val Val Leu Asp Leu He Ala 70 75 80 85 atg att ttc ggc atg cca cgt gcg ctt tac ccc gag atc gca gaa gtg 403 Met He Phe Gly Met Pro Arg Ala Leu Tyr Pro Glu He Wing Glu Val 90 95 100 aac ttc ggt ggg ggt gac gcc ggt gca acg atg ctg gcg ttc atg tac 451 Asn Phe Gly Gly Gly Asp Wing Gly Wing Thr Met Leu Wing Phe Met Tyr 105 110 115 tea tcc atg gct gtt ggc gca gtt ctt ggc ggc gtg ctg tct ggt tgg 499 Ser Ser Met Wing Val Gly Wing Val Leu Gly Gly Val Leu Ser Gly Trp 120 125 130 gtg gcc cgg att age cgc cag ggt gtt gca gtt tat tgg tgc atc atc 547 Val Ala Arg He Ser Arg Gln Gly Val Ala Val Tyr Trp Cys He He 135 140 145 gcc tgg ggc gca gcc gtt gct gt tg ggt gg gg gca att gtt gtc age 595 Wing Trp Gly Wing Wing Val Wing Gly Le Val Gly Gly Val Wing Val Val 150 150 160 160 ccc gcg gcg gtg gcg tgg ggg ggg ggg tg tg atc atc atc atg atg gtc 643 Pro Gly Wing Val Thr Wing Trp Wing Trp Met Phe He Met Met Met Val 170 175 180 att ggt ggc atg gct gac atg ttc age tcg gca gtt cga aac gct att 691 He Gly Gly Met Wing Asp Met Phe Ser Ser Wing Val Arg Asn Wing 185 190 195 ttg cag cag tct gct gcg gaa cat gtg cag ggc cga atc caa ggt gtg 739 Leu Gln Gln Ser Ala Ala Glu Hís Val Gln Gly Arg He Gln Gly Val 200 205 210 tgg atc atc gtc gtg gtg ggt gga ect cgt tta gct gac gtc ctt cac 787 Trp He He Val Val Gly Gly Pro Arg Leu Ala Asp Val Leu His 215 220 225 ggt tgg gcc gct gag ccc ctc ggc gca ggt tgg acg gta tta tgg ggc 835 Gly Trp Ala Wing Glu Pro Leu Gly Wing Gly Trp Thr Val Leu Trp Gly 230 235 240 245 gga gta gcg gtg gtt gta ctc act gca att tgt atg gtg gcg gtg ect 883 Gly Val Ala Val Val Val Leu Thr Ala He Cys Met Val Val Val Pro 250 255 260 aaa ttc tgg aaa tac gag aaa cca aaa att acc ggc atc taaatactta 932 Lys Phe Trp Lys Tyr Glu Lys Pro Lys He Thr Gly He 265 270 tccatgccca ttt 945 < 210 > 170 < 211 > 274 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 170 Met Leu Leu Met Gln Thr Gly Ala He Val Gly Pro Leu He Ala Gly 1 5 10 15 Ala Leu He Pro Leu He Gly Phe Gly Trp Leu Tyr Phe Leu Asp Val 20 25 30 Val Ser He He Pro Thr Leu Trp Ala Val Trp Ser Leu Pro Be He 35 40 45 • Lys Pro Ser Gly Lys Val Met Lys Wing Gly Phe Wing Ser Val Val Asp 50 55 60 Gly Leu Lys Tyr Leu Wing Gly Gln Pro Val Leu Leu Met Val Met Val 65 70 75 80 Leu Asp Leu He Wing Met He Phe Gly Met Pro Arg Wing Leu Tyr Pro 85 90 95 Glu He Wing Glu Val Asn Phe Gly Gly Gly Asp Wing Gly Wing Thr Met 10 100 105 110 Leu Wing Phe Met Tyr Ser Being Met Wing Val Gly Wing Val Leu Gly Gly 115 120 125 Val Leu Ser Gly Trp Val Wing Arg He Ser Arg Gln Gly Val Wing Val 130 135 140 Tyr Trp Cys He He Wing Trp Gly Wing Wing Val Wing Leu Gly Gly Val 145 150 155 160 Ala He Val Val Ser Pro Gly Ala Val Thr Ala Trp Ala Trp Met Phe 15 165 170 175 He He Met Met Val He Gly Gly Met Wing Asp Met Phe Ser Wing 180 185 190 Val Arg Asn Wing He Leu Gln Gln Ser Wing Wing Glu His Val Gln Gly 195 200 205 Arg He Gln Gly Val Trp He He Val Val Val Gly Gly Pro Arg Leu 210 215 220 Wing Asp Val Leu His Gly Trp Wing Wing Glu Pro Leu Gly Wing Gly Trp 20 225 230 235 • 240 Thr Val Leu Trp Gly Gly Val Wing Val Val Val Leu Thr Ala He Cys 245 250 255 Val Val Val Pro Lys Phe Trp Lys Tyr Glu Lys Pro Lys He Thr 260 265 270 Gly He 25 < 210 > 171 < 211 > 549 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (526) < 223 > FRXA01937 5 < 400 > 171 gcgcggtgac accacagccg ttgtcagcgg cgcttggtct gtggaggatc gccgaggtta 60 ctaacaaata ggcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115 Val Ser Phe Arg Asp 1 5 att ttc gct gac acc aga ccg ctg aaa gaa ccg gcc ttc aaa cgc ctc 163 He Phe Wing Asp Thr Arg Pro Leu Lys Glu Pro Wing Phe Lys Arg Leu 10 15 20 10 tgg ctt ggc aat gtt gcc gtc acc att ggt gcc caa tta act gtt gtt 211 Trp Leu Gly Asn Val Wing Thr Val He Gly Wing Gln Leu Thr Val Val 25 30 35 • gcc gtt ccg gtg cag att tac caa atg act ggg tcc ggc tat gtg 259 Wing Val Pro Val Gln He Tyr Gln Met Thr Gly Ser Ser Gly Tyr Val 40 45 50 ggc ttg acc ggg ctt ttt ggc ctt att ect ttg gtt att ttt ggc ctt 307 Gly Leu Thr Gly Leu Phe Gly Leu He Pro Leu Val He Phe Gly Leu 55 60 65 15 tat ggt gga tcc att gcg gat gct ttt gat aaa cgc atc gtg ctg atc 355 Tyr Gly Gly Ser He Wing Asp Wing Phe Asp Lys Arg He Val Leu He 70 75 80 85 tgc acc acg atc ggc atg tgt gtc acc act gcc gtt ttt tgg gtg ctg 403 Cys Thr Thr He Gly Met Cys Val Thr Thr Ala Gly Phe Trp Val Leu 90 95 • 100 acc att tta ggc aat gag aat att tgg ctc ctg tta ata aac ttt tct 451 • Thr He Leu Gly Asn Glu Asn He Trp Leu Leu Leu He Asn Phe Ser 105 110 115 20 tta cag cag gca ttt ttc gcg gtg aat caa ccc acc acga gcg atc 499 Leu Gln Gln Ala Phe Phe Ala Val Asn Gln Pro Thr Arg Thr Ala He 120 125 130 ctt cga agt att ttg ccg att gat caa taagcgtcgg caacatcact 546 Leu Arg Ser He Leu Pro He Asp Gln 135 140 gaa 549 < 210 > 172 25 < 211 > 142 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 172 Val Ser Phe Arg Asp He Phe Wing Asp Thr Arg Pro Leu Lys Glu Pro 1 5 10 15 Wing Phe Lys Arg Leu Trp Leu Gly Asn Val Wing Thr Val He Gly Wing 20 25 30 Gln Leu Thr Val Val Wing Val Pro Val Gln He Tyr Gln Met Thr Gly 35 40 45 Ser Ser Gly Tyr Val Gly Leu Thr Gly Leu Phe Gly Leu He Pro Leu 50 55 60 Val He Phe Gly Leu Tyr Gly Gly Ser He Wing Asp Wing Phe Asp Lys 65 70 75 80 Arg He Val Leu He Cys Thr Thr He Gly Met Cys Val Thr Thr Wing 85 90 95 Gly Phe Trp Val Leu Thr He Leu Gly Asn Glu Asn He Trp Leu Leu 100 105 110 Leu He Asn Phe Ser Leu Gln Gln Wing Phe Phe Wing Val Asn Gln Pro 115 120 125 Thr Arg Thr Wing He Leu Arg Ser He Leu Pro He Asp Gln 130 135 140 < 210 > 173 < 211 > 1242 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1219) < 223 > RXN01010 <; 400 > 173 gtgccaaagc gtttcctgta aaacgcataa ccccgaatac cccctgtttc cagatccaaa 60 aaaagatctg gcagggggtt taggcataga ttaggaactt atg aag aaaa ctg caa 115 Met Lys Lys Leu Gln 1 5 atg ccg gcc att ttg gtc gga ggc ttt gtg ggg ccg ttt act ggc caa 163 Met Pro Ala He Leu Val Gly Gly Phe Val Gly Pro Phe Thr Gly Gln 10 15 20 gct cta tea gtg gtc ttg ccg gaa ttt gca gac acc ttt gat atc agt 211 Wing Leu Ser Val Val Leu Pro Glu Phe Wing Asp Thr Phe Asp He Ser 25 30 35 gtc age cag gca gcg ctg acc atg action gca tac ttg ttg ccc ttt gcc 259 Val Ser Gln Wing Wing Leu Thr Met Thr Wing Tyr Leu Leu Pro Phe Wing 40 45 50 at ttg ttg ttt tcg ggg cgc atc ac aga aac atc cat ccg cat 307 Thr Met Met Leu Phe Ser Gly Arg He Thr Arg Lys íle His Pro His 55 60 65 aag gtg gtg cag gcg gct tat att gtc aca ctg cca ctt gcg ctg ttg 355 Lys Val Val Gln Ala Ala Tyr He Val Thr Leu Pro Leu Ala Leu Leu 70 75 80 85 ctc cta gtt aca cca tcg tgg ggg ctg ttt atg gct gcg tat gcc acg 403 Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met Wing Ala T yr Ala Thr 90 95 100 att ggt atc gct aat gca ttt acc act ccg gtg ctg caa att atg ttg 451 He Gly He Wing Asn Wing Phe Thr Pro Val Leu Gln He Met Leu 105 110 115 cgt gag ctt gtt ccg ccg cgt tct ttg ggt aag gca ttg ggc acc tat 499 Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys Ala Leu Gly Thr Tyr 120 125 130 gct gcg atg caa tea ctc ggc atg ttg tcg gcg cca ctg atc gca ggt 547 Ala Ala Met Gln Ser Leu Gly Met Leu Ser Wing Pro Leu He Wing Gly 135 140 145 gtg tct tcg gtg gtg tcg tgg agg ttg acc ttc ctg gtc act gca gca 595 Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe Leu Val Thr Ala Ala 150 155 160 165 gcg tea ctg ttt att ttg gtg gcg cga ctc cct gtt gtt cca cca cca cca 643 Wing Ser Leu Phe He Leu Val Wing Arg Leu Pro Val Val Pro Pro Pro 170 175 180 tea gca tcg aag caa aac gtt agt ggc aag gtg cag tgg gga ccg acc 691 Be Ala Be Lys Gln Asn Val Be Gly Lys Val Gln Trp Gly Pro Thr 185 190 195 atc atc cac atg gtt tc ggc ttt gtg gtg ggc atc ggc atc atc ggc 739 He He His Met Val Ser Gly Val Val Val Gly He Gly He He Gly 200 205 '210 att gga ttc atg aca tcg ctg cac gtt ggc gag caa ttc gga ctt gat 787 He Gly Phe Met Thr Ser Leu His Val Gly Glu Gln Phe Gly Leu Asp 215 220 225 gct gca gcg cgt ggt ttg gtg gtc atg tgt ggt ggc ctg gct gcg ttc 835 Wing Wing Wing Arg Gly Leu Val Val Met Cys Gly Gly. Leu Ala Ala Phe 230 235 240 245 ttt gcc tcc cgc aag att ggc gat ttg gca gac aaa ttt ggt gtg cgc 883 Phe Wing Being Arg Lys He Gly Asp Leu Wing Asp Lys Phe Gly Val Arg 250 255 260 gcg gtg ctc att gtc agt gct gtc atc ggt acc atc gca ctc gca ctg 931 Wing Val Leu He Val Val Wing Wing He Gly Thr He Wing Leu Wing Leu 265 270 275 ctg ccg atc gc ccg tgg atc att gtg gtg gcc gta ctg tgg gcc ttc 979 Leu Pro He Wing Pro Trp He He Val Val Wing Val Leu Trp Wing Phe 280 285 290 gca gta gca gca gca gca caa gga atc caca gca acc gtc aac ttg gct gtc 1027 Ala Ala Ala Ala Ala Gln Gly He Gln Ala Thr Val Asn Leu Ala Val 295 300 305 atc gga age ccc ggt gga tea tcg ctg ctt tct acc gtg cag gct ttc 1075 He Gly Ser Pro Gly Gly Ser Ser Leu Leu Ser Thr Val Gln Ala Phe 310 315 320 325 cga ttc ttc gga tea gcg gca gca cca gtg aca ttc ctt ect atc tat 1123 Arg Phe Phe Gly Be Ala Ala Ala Pro Val Thr Phe Leu Pro He Tyr 330 335 340 atg ggc atc ggc tcg ggg gcg ttt tgg gtc age gcg gta gcg ctg ttc 1171 Met Gly He Gly Ser Gly Ala Phe T rp Val Ser Wing Val Wing Leu Phe 345 350 355 ttc gtt gcc atc gcc cag tgg ctc aac ccg cag cgg gtg gag cgg ggc 1219 Phe Val Wing Wing Gln Trp Leu Asn Pro Gln Arg Val Glu Arg Gly 360 365 370 tgagggagac gtegagaage gtc 1242 < 210 > 174 < 211 > 373 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 174 Met Lys Lys Leu Gln Met Pro Ala He Leu Val Gly Gly Phe Val Gly 1 5 10 15 Pro Phe Thr Gly Gln Ala Leu Ser Val Val Leu Pro Glu Phe Ala Asp 20 25 30 Thr Phe Asp He Ser Val Ser Gln Ala Ala Leu Thr Met Thr Wing Tyr 35 40 45 Leu Leu Pro Phe Wing Thr Met Met Leu Phe Ser Gly Arg He Thr Arg 50 55 60 Lys He His Pro His Lys Val Val Gln Ala Wing Tyr He Val Thr Leu 65 70 75 80 Pro Leu Wing Leu Leu Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met 85 90 95 Wing Wing Tyr Wing Thr He Gly Wing Wing Asn Wing Phe Thr Thr Pro Val 100 105 110 Leu Gln He Met Leu Arg Glu Leu Val Pro Pro Arg Ser Leu Gly Lys 115 120 125 Wing Leu Gly Thr Tyr Wing Wing Met Gln Ser Leu Gly Met Leu Ser Wing 130 135 140 Pro Leu He Wing Gly Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe 145 150 155 160 Leu Val Thr Ala Ala Ala Ser Leu Phe He Leu Val Ala Arg Leu Pro 165 170 175 Val Val Pro Pro Pro Be Wing Ser Lys Gln Asn Val Ser Gly Lys Val 180 185 190 Gln Trp Gly Pro Thr He He His Met Val Ser Gly Phe Val Val Gly 195 200 205 He Gly He He Gly He Gly Phe Met Thr Ser Leu His Val Gly Glu 210 215 220 Gln Phe Gly Leu Asp Ala Ala Ala Arg Gly Leu Val Val Met Cys Gly 225 230 235 240 Gly Leu Ala Ala Phe Phe Ala Ser Arg Lys He Gly Asp Leu Ala Asp 245 250 255 Lys Phe Gly Val Arg Ala Val Leu He Val Ser Ala Val He Gly Thr 260 265 270 He Ala Leu Ala Leu Leu Pro He Ala Pro Trp He He Val Val Ala 275 280 285 Val Leu Trp Ala Phe Ala Ala Ala Ala Ala Gln Gly He Gln Ala Thr 290 295 300 Val Asn Leu Ala Val He Gly Ser Pro Gly Gly Ser Ser Leu Leu Ser 305 310 315 320 Thr Val Gln Wing Phe Arg Phe Phe Gly Wing Wing Wing Pro Val Thr 325 330 335 Phe Leu Pro He Tyr Met Gly He Gly Ser Gly Wing Phe Trp Val Ser 340 345 350 Wing Val Wing Leu Phe Phe Val Wing Wing Gln Trp Leu Asn Pro Gln 355 360 365 Arg Val Glu Arg Gly 370 < 210 > 175 < 211 > 871 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (871) < 223 > FRXA01010 < 400 > 175 gtgccaaagc gtttcctgta aaacgcataa ccccgaatac cccctgtttc cagatccaaa 60 aaaagatctg gcagggggtt taggcataga ttaggaactt atg aag aaaa ctg caa 115 Met Lys Lys Leu Gln 1 5 atg ccg gcc att ttg gtc gga ggc ttt gtg ggg ccg ttt act ggc caa 163 Met Pro Ala He Leu Val Gly Gly Phe Val Gly Pro Phe Thr Gly Gln 10 15 20 gct cta tea gtg gtc ttg ccg gaa ttt gca gac acc ttt gat atc agt 211 Wing Leu Ser Val Val Leu Pro Glu Phe Wing Asp Thr Phe Asp He Ser 25 30 35 gtc age cag gca gcg ctg acc atg action gca tac ttg ttg ccc ttt gcc 259 Val Ser Gln Wing Wing Leu Thr Met Thr Wing Tyr Leu Leu Pro Phe Wing 40 45 50 at ttg ttg ttt tcg ggg cgc atc ac aga aac atc cat ccg cat 307 Thr Met Met Leu Phe Ser Gly Arg He Thr Arg Lys He His Pro His 55 60 65 aag gtg gtg cag gcg gct tat att gtc aca ctg cca ctt gcg ctg ttg 355 Lys Val Val Gln Ala Wing Tyr He Val Thr Leu Pro Leu Wing Leu Leu 70 75 80 85 ctc cta gtt aca cca tcg tgg ggg ctg ttt atg gct gcg tat gcc acg 403 Leu Leu Val Thr Pro Ser Trp Gly Leu Phe Met Wing Wing Tyr Ala Thr 90 95 100 att ggt atc gct aat gca ttt acc act ccg gtg ctg caa att atg ttg 451 He Gly He Wing Asn Wing Phe Thr Thr Pro Val Leu Gln He Met Leu 105 110 115 cgt gag ctt gtt ccg ccg cgt tct tg ggt aag gca ttg ggc acc tat 499 Arg Glu Leu Val Pro Pro Arg Be Leu Gly Lys Wing Leu Gly Thr Tyr 120 125 130 gct gcg atg caa tea ctc ggc atg ttg tcg gcg cca ctg atc gca ggt 547 Wing Ala Met Gln Ser Leu Gly Met Leu Ser Wing Pro Leu He Wing Gly 135 140 145 gtg tct tcg gtg gtg tcg tgg agg ttg acc ttc ctg gtc act gca gca 595 Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe Leu Val Thr Wing Ala 150 155 160 165 gcg tea ctg ttt att ttg gtg gcg cga ctc ccc gtt gtt cca cca cca cca 643 Wing Ser Leu Phe He Leu Val Wing Arg Leu Pro Val Val Pro Pro Pro 170 175 180 tea gca ttg aag caa aac gtt agt ggc aag gtg cag tgg gga ccg acc 691 Ser Ala Leu Lys Gln Asn Val Ser Gly Lys Val Gln Trp Gl and Pro Thr 185 190 195 atc atc cac atg gtt tcc ggc ttt gtg gtg ggc atc ggc atc atc ggc 739 He He Met His Met Val Ser Gly Phe Val Val Gly He Gly He He Gly 200 205 210 att gga ttc atg aca tcg ctg cac gtt ggc gag caa ttc gga ctt aat 787 He Gly Phe Met Thr Ser Leu His Val Gly Glu Gln Phe Gly Leu Asn 215 220 225 act gca gcg cgt ggt ttg gtg gtc atg tgt ggt ggc cgg gct gcg ttc 835 Thr Ala Ala Arg Gly Leu Val Val Met Cys Gly Gly Arg Ala Ala Phe 230 235 240 245 ttt gcc tcc cgc aag ata ggc gat ttg gca gac aaa 871 Phe Wing Being Arg Lys He Gly Asp Leu Wing Asp Lys 250 255 < 210 > 176 < 211 > 257 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 176 Met Lys Lys Leu Gln Met Pro Wing He Leu Val Gly Gly Phe Val Gly 1 5 10 15 Pro Phe Thr Gly Gln Ala Leu Ser Val Val Leu Pro Glu Phe Wing Asp 20 25 30 Thr Phe Asp He Ser Val Ser Gln Ala Wing Leu Thr Met Thr Wing Tyr 35 40 45 Leu Leu Pro Phe Wing Thr Met Met Leu Phe Ser Gly Arg He Thr Arg 50 55 60 Lys He His Pro His Lys Val Val Gln Ala Wing Tyr He Val Thr Leu 65 70 75 80 Pro Leu Ala Leu Leu Leu Leu Val Thr Pro Be Trp Gly Leu Phe Met 85 90 95 Wing Wing Tyr Wing Thr He Gly He Wing Asn Wing Phe Thr Thr Pro Val 100 105 110 Leu Gln He Met Leu Arg Glu Leu Val Pro Pro Arg Be Leu Gly Lys 115 120 125 Wing Leu Gly Thr Tyr Wing Wing Met Gln Ser Leu Gly Met Leu Ser Wing 130 135 140 Pro Leu He Wing Gly Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe 145 150 155 160 Leu Val Thr Ala Ala Ala Be Leu Phe He Leu Val Ala Arg Leu Pro 165 170 175 Val Val Pro Pro Pro Be Ala Leu Lys Gln Asn Val Ser Gly Lys Val 180 185 190 Gln Trp Gly Pro Thr He He His Met Val Ser Gly Phe Val Val Gly 195 200 205 He Gly He He Gly He Gly Phe Met Thr Ser Leu His Val Gly Glu 210 215 220 Gln Phe Gly Leu Asn Thr Ala Wing Arg Gly Leu Val Val Met Cys Gly 225 230 235 240 Gly Arg Ala Ala Phe Phe Ala Ser Arg Lys He Gly Asp Leu Ala Asp 245 250 255 Lys < 210 > 177 < 211 > 1266 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1243) < 223 > RXN03142 < 400 > 177 gcaatttggc caatcaacaa cataggagga ctgcgtggcg gtcaccgcaa gaacggacat 60 aaaaccacac ccatcgcaac caacggcact gttcactcca gtg ttt att ttg ggc 115 Val Phe He Leu Gly 1 5 tgg ctc gtc aac ttg acc cag tac ttg age ttc tac ttc ctg atc aca 163 Trp Leu Val Asn Leu Thr Gln Tyr Leu Ser Phe Tyr Phe Leu He Thr 10 15 20 gtc atg gcg ctg tat gcg atg gaa age tcc gcc gtt tea gag gcc gct 211 Val Met Ala Leu Tyr Ala Met Glu Ser Phe Ala Val Ser Glu Ala Ala 25 30 35 gtc gga ttt gcg gcc age tcc ttt gtt atc ggc gca acc gtg gct cgt 259 Val Gly Phe Wing Wing Being Ser Phe Val He Gly Wing Thr Val Wing Arg 40 45 50 gtg ttc gcg gga tgg acg tcc gac cgt ttt ggt aaa aaa cag atc ctg 307 Val Phe Wing Gly Trp Thr Ser Asp Arg Phe Gly Lys Lys Gln He Leu 55 60 65 ctc atc ttt gtc ggc ttg gaa gcg gta gca tea cta ttc tat att cca 355 Leu He Phe Val Gly Leu Glu Ala Val Ala Ser Leu Phe Tyr He Pro 70 75 80 85 gct gcc tea cta cca gcg ctg gtt gct gtg cgt ttt gtt cac ggt ttt 403 Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg Phe Val His G ly Phe 90 95 100 tct tat tct ctt gct tcc acc gct gtg atg gca ctt gtg cag tcc gtg 451 Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser Val 105 110 115 • att ect gca age cgt agg gca gag ggc acc ggc tac tcc gcg ctc gga 499 He Pro Wing Wing Arg Arg Wing Glu Gly Thr Gly Tyr Phe Wing Leu Gly 120 125 130 tcc here ctg gct aca gct tc cc gca att gcg ctg ttt gtt atc 547 Ser Thr Leu Wing Thr Wing Phe Gly Pro Wing Wing Wing Leu Phe Val He 135 140 145 gat gac ttc aac tac aac ac ctg ttc tgg att acc act gcg acc agt 595 Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp He Thr Thr Wing Thr Ser 150 155 160 165 10 gtt ttc ggc ctg atc ctc acc gtt ttg atc cgc aag ccg gag ttc att 643 Val Phe Gly Leu He Leu Thr Val Leu He Arg Lys Pro Glu Phe He 170 175 180 aag aat gcg gaa cac ggc aga gta aag cca gtc tgg tct atc aag act 691 Lys Asn Wing Glu His Gly Arg Val Lys Pro Val Trp Ser He Lys Thr 185 190 195 gtt gtg cac cca tcg gtc atg ctc att gga ttc ttc atg ctc gct gtc 739 Val Val His Pro Ser Val Me t Leu He Gly Phe Phe Met Leu Ala Val 200 205 210 15 gga ctg gct tac gca ggc gtg atc acc ttc ctc aac ggc ttc gcg caa 787 Gly Leu Ala Tyr Ala Gly Val He Thr Phe Leu Asn Gly Phe Ala Gln 215 220, 225 gac act ggc ctc acc gcc gcg ggt ctt ttc ttt atc gct tat gcg 835 Asp Thr Gly Leu Thr Wing Gly Wing Gly Leu Phe Phe He Wing Wing Tyr Wing 230 235 240 245 gtt gcg atg ctg gtc atg cgt ttc ttc ctt gga cgc att cag gac aaa 883 Val Ala Met Leu Val Met Arg Phe Phe Leu Gly Arg He Gln Asp Lys 250 255 260 20 cat ggt gac aac ccg gtt att tac ttc ggt ttg atc age tcc gcc ctc 931 His Gly Asp Asn Pro Val He Tyr Phe Gly Leu He Ser Phe Ala Leu 265 270 * 275 gcg ctg ggg ctt atg gct ttg gcg act gaa gac tgg cac att gtt ctc 979 Wing Leu Gly Leu Met Wing Ala Leu Thr Glu Asp Trp His He Val Leu 280 285 290 gct ggc gca ctc acc ggt tg ggc tat ggc acc atc atc ccg gcc gca 1027 Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr He Met Pro Ala Ala 295 300 305 25 - - - ^ '' - ^ iBítp f ni "caa gcc att gct gtc gatte gtt cca age act cag gtt ggt tcc ggt 1075 Gln Ala He Ala Val Asp Ser Val Pro Ser Thr Gln Val Gly Ser Gly 310 315 320 325 att tct acg ctt ttc ctg ttc acc gac atc ggc att ggc tta ggc cca 1123 I've been Thr Leu Phe Leu Phe Thr Asp He Gly He Gly Leu Gly Pro 330 335 340 atc ctg ctg ggt gga ttg gtt gca gcg acc gga tac aac gtc atg tac 1171 He Leu Leu Gly Gly Leu Val Wing Wing Thr Gly Tyr Asn Val Met Tyr 345 350 355 gca gct ttg gcc gca gtg att gtt gtg gcg ggc gtg ctc tac ctg gtt 1219 Ala Ala Leu Ala Ala Val He Val Val Ala Gly Val Leu Tyr Leu Val 360 365 370 gct ttg ggt agg aaa gct age cac taagttagag cattttattg age 1266 Ala Leu Gly Arg Lys Ala Ser His 375 380 < 210 > 178 < 211 > 381 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 178 Val Phe He Leu Gly Trp Leu Val Asn Leu Thr Gln Tyr Leu Ser Phe 1 5 10 15 Tyr Phe Leu He Thr Val Met Wing Leu Tyr Wing Met Glu Ser Phe Wing 20 25 30 Val Ser Glu Wing Wing Val Gly Phe Wing Wing Be Ser Phe Val He Gly 35 40 45 Wing Thr Val Wing Arg Val Phe Wing Gly Trp Thr Ser Asp Arg Phe Gly 50 55 60 Lys Lys Gln He Leu Leu He Phe Val Gly Leu Glu Wing Val Wing Ser 65 70 75 80 Leu Phe Tyr He Pro Wing Wing Ser Leu Pro Wing Leu Val Wing Val Arg 85 90 95 Phe Val His Gly Phe Ser Tyr Ser Leu Wing Ser Thr Wing Val Met Wing 100 105 110 Leu Val Gln Ser Val He Pro Wing Ser Arg Arg Wing Glu Gly Thr Gly 115 120 125 Tyr Phe Wing Leu Gly Ser Thr Leu Wing Thr Wing Phe Gly Pro Wing He 130 135 140 Wing Leu Phe Val He Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp He 145 150 155 160 Thr Thr Wing Thr Ser Val Phe Gly Leu He Leu Thr Val Leu He Arg 165 170 175 Lys Pro Glu Phe He Lys Asn Wing Glu His Gly Arg Val Lys Pro Val 180 185 190 Trp Ser He Lys Thr Val Val His Pro Ser Val Met Leu He Gly Phe 195 200 205 Phe Met Leu Ala Val Gly Leu Ala Tyr Ala Gly Val He Thr Phe Leu 210 215 220 Asn Gly Phe Ala Gln Asp Thr Gly Leu Thr Ala Gly Ala Gly Leu Phe 225 230 235 240 Phe He Wing Tyr Wing Val Wing Met Leu Val Met Arg Phe Phe Leu Gly 245 250 255 Arg He Gln Asp Lys His Gly Asp Asn Pro Val He Tyr Phe Gly Leu 260 265 270 He Be Phe Ala Leu Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp 275 280 285 Trp His He Val Leu Ala Gly Ala Leu Thr Gly Leu Gly Tyr Gly Thr 290 295 300 He Met Pro Wing Wing Gln Wing He Wing Val Asp Ser Val Pro Ser Thr 305 310 315 320 Gln Val Gly Ser Gly Be Ser Thr Leu Phe Leu Phe Thr Asp He Gly 325 330 335 He Gly Leu Gly Pro He Leu Leu Gly Gly Leu Val Wing Ala Thr Gly 340 345 '350 Tyr Asn Val Met Tyr Ala Ala Leu Ala Ala Val He Val Val Ala Gly 355 360 - 365 Val Leu Tyr Leu Val Ala Leu Gly Arg Lys Ala Ser His 370 375 380 < 210 > 179 < 211 > 914 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (891) < 223 > FRXA01150 < 400 > 179 cca gct gcc tea cta cca gcg ctg gtt gct gtg cgt ttt gtt cac ggt 48 Pro Ala Ala Ser Leu Pro Ala Leu Val Ala Val Arg Phe Val His Gly 1 5 10 15 ttt tct tct tat tct ctt gct tcc gct gtg gtg tc gtg gtg ctc ct gtg 96 Phe Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser 20 25 30 gtg att ect gca age cgt agg gca gag ggc acc ggc tac tcc gcg ctc 144 Val He Pro Wing Wing Arg Arg Wing Glu Gly Thr Gly Tyr Phe Wing Leu 35 40 45 gga tcc aca ctg gct aca gct tc gcc gca att gcg ctg ttt gtt 192 Gly Ser Thr Leu Wing Thr Wing Phe Gly Pro Ala.'He Ala Leu Phe Val 50 55 60 atc gat gac ttc aac tac aac ctg ttc tgg att acc act gcg acc 240 He Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp He Thr Thr Ala Thr 65 70 75 80 agt gtt ttc ggc ctg atc ctc acc gtt ttg atc cgc aag ccg gag ttc 288 Ser Val Phe Gly Leu He Leu Thr Val Leu He Arg Lys Pro Glu Phe 85 90 95 att aag aat gcg gaa cac ggc aga gta aag cca gtc tgg tct atc aag 336 He Lys Asn Wing Glu His Gly Arg Val Lys Pro Val Trp Ser He Lys 100 105 110 act gtt gtg cac cca tcg gtc atg ctc att gga ttc ttc atg ctc gct 384 Thr Val Val His Pro Ser Val Met Leu He Gly Phe Phe Met Leu Wing 115 120 125 gtc gga ctg gct tac gca ggc gtg atc acc ttc ctc aac ggc tc gcg 432 Val Gly Leu Wing Tyr Wing Gly Val He Thr Phe Leu Asn Gly Phe Wing 130 135 14? ' caa gac act ggc ctc acc gcc gga gcg gtt ctt ttc ttt atc gct tat 480 Gln Asp Thr Gly Leu Thr Ala Gly Ala. Gly Leu Phe Phe He Ala Tyr 145 150 155, 160 gcg gtt gcg atg ctg gtc atg cgt ttc ttc ctt gga cgc att cag gac 528 Ala Val Ala Met Leu Val Met Arg Phe Phe Leu Gly Arg He Gln Asp 165 170 175 aaa cat ggt gac aac ccg gtt att tac ttc ggt ttg atc age tcc gcc 576 Lys His Gly Asp Asn Pro Val He Tyr Phe Gly Leu He Ser Phe Wing 180 185 190 ctc gcg ctg ggg ctt atg gct ttg gcg act gaa gac tgg cac att gtt 624 Leu Ala Leu Gly Leu Met Ala Leu Ala Thr Glu Asp Trp His He Val 195 200 205 ctc gct ggc gca ctc acc ggt ttg ggc tat ggc atc atc ccg gcc 672 Leu Wing Gly Wing Leu Thr Gly Leu Gly Tyr Gly Thr He Met Pro Wing 210 215 220 gca caa gcc att gct gtc gat tea gtt cca age act cag gtt ggt tcc 720 Wing Gln Ala Wing Wing Val Asp Ser Val Pro Ser Thr. Gln Val Gly Ser 225 230 235 240 ggt att tct acg ctt ttc ctg ttc acc gac atc ggc att ggc tta ggc 768 Gly He Ser Thr Leu Phe Leu Phe Thr Asp He Gly He Gly Leu Gly 245 250 255 cca atc ctg ctg ggt gga ttg gtt gca gcg acc gga tac aac gtc atg 816 Pro He Leu Leu Gly Gly Leu Val Wing Wing Thr Gly Tyr Asn Val Met 260 265 270 tac gca gct tcg gcc gca gtg att gtt gtg gcg ggc gtg ctc tac ctg 864 Tyr Ala Ala Leu Ala Ala Val He Val Val Ala , Gly Val Leu Tyr Leu 275 280 285 gtt gct ttg ggt agg aaa gct age cac taagttagag cattttattg 911 Val Ala Leu Gly Arg Lys Ala Ser His 290 295 age 914 < 210 > 180 < 211 > 297 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 180 Pro Ala Ala Be Leu Pro Ala Leu Val Ala Val Arg Phe Val His Gly 1 5 10 15 Phe Ser Tyr Ser Leu Ala Ser Thr Ala Val Met Ala Leu Val Gln Ser 20 25 30 Val He Pro Ala Ser Arg Arg Ala Glu Gly Thr Gly Tyr Phe Wing Leu 35 40, 45 Gly Ser Thr Leu Wing Thr Wing Phe Gly Pro Wing Wing Wing Leu Phe Val 50 55 60 He Asp Asp Phe Asn Tyr Asn Thr Leu Phe Trp? Le Thr Thr Wing Thr 65 70 75. 80 Ser Val Phe Gly Leu He Leu Thr Val Leu He Arg Lys Pro Glu Phe 85 90 95 He Lys Asn Wing Glu His Gly Arg Val Lys Pro Val Trp Ser He Lys 100 105 110 Thr Val Val His Pro Ser Val Met Leu He Gly Phe Phe Met Leu Wing 115 120 125 Val Gly Leu Wing Tyr Wing Gly Val He Thr Phe Leu Asn Gly Phe Wing 130 135 i40 Gln Asp Thr Gly Leu Thr Wing Gly Wing Gly Leu Phe Phe He Wing Tyr 145 150 155 160 Wing Val Wing Met Leu Val Met Arg Phe Phe Leu Gly Arg He Gln Asp 165 170 175 Lys His Gly Asp Asn Pro Val He Tyr Phe Gly Leu He Ser Phe Wing 180 185 190 Leu Wing Leu Gly Leu Met Wing Leu Wing Thr Glu Asp Trp His He Val 195 200 205 Leu Wing Gly Wing Leu Thr Gly Leu Gly Tyr Gly Thr He Met Pro Wing 210 215 220 Wing Gln Wing Wing Wing Val Asp Ser Val Pro Ser Thr Gln Val Gly Ser 225 230 235 240 Gly He Ser Thr Leu Phe Leu Phe Thr Asp He Gly He Gly Leu Gly 245 250 - 255 Pro He Leu Leu Gly Le Val Val Ala Wing Thr Gly Tyr Asn Val Met 260 265 270 Tyr Wing Wing Leu Wing Wing Val He Val Val Wing Gly Val Leu Tyr Leu 275 280 285 Val Ala Leu Gly Arg Lys Ala Ser His 290 295 < 210 > 181 < 211 > 1341 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1318) < 223 > RXN02964 < 400 > 181 ttttatatcc tagcaagggt gttgcatgat gcataaacg tggtagtttg tgttcataac 60 aaaattgcat gatgcaataa tttcgattta aaggagaaca gtg tcc gta gct gaa 115 Val Ser Val Ala Glu 1 5 gaa ggg aaa ctt ttt aca cca acg ttt gtc atg gga tgg ttt gcc aac 163 Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met Gly Trp Phe Wing Asn 10 15 20 ctt ttc cag ttc ctg gtg ttc tac ttc ctc atc acc acc atg gct ttg 211 Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu He Thr Thr Met Ala Leu 25 30 35 tac gcc atc aag gaa ttt caa gcc tct gaa gta gaa gct ggc tcc gca 259 Tyr Wing He Lys Glu Phe Gln Wing Wing Glu Val Glu Wing Gly Phe Wing 40 45 50 tcc age tea att gtt atc ggc gca gtc ttt tcc agg ttt ttc tcc ggc 307 Be Ser Be He Val He Gly Ala Val Phe Be Arg Phe Phe Ser Gly 55 60 65 tat att att gac cgt ttt ggt cga cgc aag att < gtg ctc atc tea gtc 355 Tyr He He Asp Arg Phe Gly Arg Arg Lys He Val Leu He Ser Val 70 75 80 85 cta gtc act acc att gcg tgt gcc ttg tac ctt ccc atc gaa tea ttg 403 Leu Val Thr Thr He Ala Cys Ala Leu Tyr Leu Pro He Glu Ser Leu 90 95 100 cca ttg cta tac gca aac agg ttc ctc cac ggt gtt gga tac gct ttt 451 Pro Leu Leu Tyr Wing Asn Arg Phe Leu His Gly Val Gly Tyr Ala Phe 105 110 115 gct gcc acc gcg atc atg gca atg gtc cag gag ctc att cca gcg tea 499 Ala Ala Thr Ala He Met Ala Met Val Gln Glu Leu He Pro Wing Ser 120 125 130 cga cgt tcc gaa ggt act ggt tac ctg gca ttg ggc act acc gtt tct 547 Arg Arg Ser Glu Gly Thr Gly Tyr Leu Wing Leu Gly Thr Thr Val Ser 135 140 145 gca gca ctt gga cca gcc cta gca ctt ttt gtc cta gga aca ttt gat 595 Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val Leu Gly Thr Phe Asp 150 155 160 165 tac gac atg ctg ttt atc gtg gtc ttg gca acc tcg gtc atc tct ttg 643 Tyr Asp Met Leu Phe He Val Val Leu Ala Thr Ser Val I'm Leu 170 175 180 atc gcc gtc gtg ttc atg tac ttt aag acc age gac ect gag ect tct 691 He Ala Val Val Phe Met Tyr Phe Lys Thr Ser Asp Pro Glu Pro Ser 185 190 195 ggg gaa cca gcc aag ttc age ttc aaa tct att atg aac cca aag atc 739 Gly Glu Pro Wing Lys Phe Ser Phe Lys Ser He Met Asn Pro Lys He 200 205 210 atc ccc atc ggc atc ttt atc ttg ctt att tgc ttt gct tac tct ggc 787 He Pro He Gly He Phe He Leu Leu He Cys Phe Wing Tyr Ser Gly 215 220 225 gtc att gcc tac atc aac gca ttt gct gaa gaa cgc gat ctg att acg 835 Val He Wing Tyr He Asn Wing Phe Wing Glu Glu Arg Asp Leu He Thr 230 235 240 245 ggt gct gga ttg ttc ttc att gcc tac gca gta tea atg ttt gtg atg 883 Gly Wing Gly Leu Phe Phe He Wing Tyr Wing Val Ser Met Phe Val Met 250 255 260 cgc age ttc ctt ggc aaa ctg cag gac cgt cgc gga gac aac gtc gtt 931 Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg Gly Asp Asn Val Val 265 270 275 att tac ttt gga ttg ttc ttc ttc gtt att tcc ttg acg att ttg tcc 979 He Tyr Phe Gly Leu Phe Phe Phe Val He Ser Leu Thr He Leu Ser 280 285 290 ttt gcc act tcc aac tgg cac gtt gtg ttg tcc gga gtc att gca ggt 1027 Phe Ala Thr Ser Asn Trp His Val Val Leu Ser Gly Val He Ala Gly 295 300 305 ctg gga tac ggc act ttg atg cca gca gtg cag tcc atc gct gtt ggt 1075 Leu Gly Tyr Gly Thr Leu Met Pro Wing Val Gln Ser He Wing Val Gly 310 315 320, 325 gta gta gac aaa acc gaa ttc ggt acg gcc ttc tcc act ttc ttc ctg 1123 Val Val Asp Lys Thr Glu Phe Gly Thr Wing Phe Ser Thr Leu Phe Leu 330 335 340 ttt gtg gac tta ggt ttt ggc ttt gga ect att atc ctg gga gca gtt 1171 Phe Val Asp Leu Gly Phe Gly Phe Gly Pro He He Leu Gly Ala Val 345 350 355 tct gcg gca att ggt ttc gga ect atg tat gca gca ctg gca ggt gtg 1219 Ser Ala Ala He Gly Phe Gly Pro Met Tyr Ala Ala Leu Ala Gly Val 360 365 370 ggt gtg att gcc gtc atc ttc tac ctg ttc aca cccct cct gct acc gat 1267 Gly Val He Wing Gly He Phe Tyr Leu Phe Thr His Wing Arg Thr Asp 375 380 385 cga gct aag aat ggc ttt gtt aaa cac cca gag ect gtc gct tta gtt 1315 Arg Ala Lys Asn Gly Phe Val Lys His Pro Glu Pro Val Ala Leu Val 390 395 400 405 age tagttctttc agctttccct ccc 1341 Be < 210 > 182 < 211 > 406 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 182 Val Ser Val Ala Glu Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met 1 5 10 15 Gly Trp Phe Wing Asn Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu He 20 25 30 Thr Thr Met Ala Leu Tyr Ala He Lys Glu Phe Gln Wing Ser Glu Val 35 40 45 Glu Wing Gly Phe Wing Being Ser Val He Gly Wing Val Phe Ser 50 55 60 Arg Phe Phe Ser Gly Tyr He He Asp Arg Phe Gly Arg Arg Lys He 65 70 75 • 80 Val Leu He Ser Val Leu Val Thr Thr He Ala Cys Ala Leu Tyr Leu 85 90 95 Pro He Glu Ser Leu Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly 100 105 110 Val Gly Tyr Ala Phe Ala Ala Thr Ala He Met Ala Met Val Gln Glu 115 120 125 Leu He Pro Wing Ser Arg Arg Ser Glu Gly Thr Gly Tyr Leu Wing Leu 130 135 '140 Gly Thr Thr Val Wing Wing Wing Leu Gly Pro Wing Leu Wing Leu Phe Val 145 150 155 160 Leu Gly Thr Phe Asp Tyr Asp Met Leu Phe He Val Val Leu Ala Thr 165 170 175 Ser Val He Ser Leu He Wing Val Val Phe Met Tyr Phe Lys Thr Ser 180 185. 190 Asp Pro Glu Pro Ser Gly Glu Pro Wing Lys Phe Ser Phe Lys Ser He 195 200 '205 Met Asn Pro Lys He He Pro He Gly He Phe He Leu He He Cys 210 215 220 Phe Wing Tyr Ser Gly Val He Wing Tyr He Asn Ala Phe Ala Glu Glu 225 230 235 240 Arg Asp Leu He Thr Gly Wing Gly Leu Phe Phe He Wing Tyr Wing Val 245 250 -.- 255 Ser Met Phe Val Met Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg 260 265 ••• '270 Gly Asp Asn Val Val He Tyr Phe Gly Leu Phe Phe Phe Val He Ser 275 280 285 Leu Thr He Leu Ser Phe Wing Thr Ser Asn Trp His Val Val Leu Ser 290 295 300 Gly Val He Wing Gly Leu Gly Tyr Gly Thr Leu Met Pro Wing Val Gln 305 310 315 320 Ser He Wing Val Gly Val Val Asp Lys Thr Glu Phe Gly Thr Wing Phe 325 330 335 Ser Thr Leu Phe Leu Phe Val Asp Leu Gly Phe Gly Phe Gly Pro He 340 345 '350 He Leu Gly Wing Val Wing Wing He Gly Phe Gly Pro Met Tyr Wing 355 360 365 Wing Leu Wing Gly Val Gly Val He Wing Gly He Phe Tyr Leu Phe Thr 370 375 3 * 80 His Wing Arg Thr Asp Arg Wing Lys Asn Gly Phe Val Lys Hi s Pro Glu 385 390 395. 400 dißÉ ^ i iiái Pro Val Ala Leu Val Ser 405 < 210 > 183 < 211 > 1006 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1006) < 223 > FRXA02H6 < 400 > 183 ttttatatcc tagcaagggt gttgcatgat gcataaacg tggtagtttg tgttcataac 60 aaaattgcat gatgcaataa tttcgattta aaggagaaca gtg tcc gta gct gaa 115 Val Ser Val Ala Glu 1 5 gaa ggg aaa ctt ttt aca cca acg ttt gtc atg gga tgg ttt gcc aac 163 Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met Gly Trp Phe Wing Asn 10 15 20 ctt ttc cag ttc ctg gtg ttc tac ttc ctc atc acc acc atg gct ttg 211 Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu He Thr Thr Met Ala Leu 25 30 35 tac gcc atc aag gaa ttt caa gcc tct gaa gta gaa gct ggc tcc gca 259 Tyr Wing He Lys Glu Phe Gln Wing Wing Glu Val Glu Wing Gly Phe Wing 40 45 50 tcc age tea att gtt atc ggc gca gtc ttt tcc agg ttt ttc tcc ggc 307 Be Ser Be He Val He Gly Ala Val Phe Be Arg Phe Phe Ser Gly 55 60 65 tat att att gac cgt ttt ggt cga cgc aag att gtg ctc atc tea gtc 355 Tyr He He Asp Arg Phe Gly Arg Arg Lys He Val Leu He Ser Val 70 75 80 85 cta gtc act acc att gcg tgt gcc ttg tac ctt ccc atc gaa tea ttg 403 Leu Val Thr Thr He Ala Cys Ala Leu Tyr Leu Pro He Glu Ser Leu 90 95 100 cca ttg cta tac gca aac agg ttc ctc cac ggt gtt gga tac gct ttt 451 Pro Leu Leu Tyr Wing Asn Arg Phe Leu His Gly Val Gly Tyr Ala Phe 105 110 115 gct gcc acc gcg atc atg gca atg gtc cag gag ctc att cca gcg tea 499 Ala Ala Thr Ala He Met Ala Met Val Gln Glu Leu He Pro Wing Ser 120 125 130 cga cgt tcc gaa ggt act ggt tac ctg gca ttg ggc act acc gtt tct 547 Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu Gly Thr Thr Val Ser 135 140 145 gca gca ctt gga cca gcc cta gca ctt ttt gtc cta, gga aca ttt gat 595 Ala Ala Leu Gly Pro Ala Leu Ala Leu Phe Val Leu Gly Thr Phe Asp 150 155 160 165 tac gac atg ctg ttt atc gtg gtc gtc ttg gca acc tcg gtc atc tct ttg 643 Tyr Asp Met Leu Phe He Val Val Leu Ala Thr Ser Val He Ser Leu 170 175 180 atc gcc gtc gtg ttc atg tac ttt aag acc age gac ect gag ect tct 691 He Wing Val Val Phe Met Tyr Phe Lys Thr Ser Asp Pro Glu Pro Ser 185 190 195 ggg gaa cca gcc aag ttc age ttc aaa tct att atg aac cca aag atc 739 Gly Glu Pro Wing Lys Phe Ser Phe Lys Ser He Met Asn Pro Lys He 200 205 210 atc ccc atc ggc atc ttt atc ttg ctt att tgc ttt gct tac tct ggc 787 He Pro He Gly He Phe He Leu Leu He Cys Phe Wing Tyr Ser Gly 215 220 225 gtc att gcc tac atc aac gca ttt gct gaa gaa cgc gat ctg att acg 835 Val He Wing Tyr He Asn Wing Phe Wing Glu Glu Arg Asp Leu He Thr 230 235 240 245 ggt gct gga ttg ttc ttc att gcc tac gca gta tea atg ttt gtg atg 883 Gly Wing Gly Leu Phe Phe He Wing Tyr Wing Val Met Met Met Phe Val Met 250 255 5 260 cgc age ttc ctt ggc aaa ctg cag gac cgc cgc gga gac aac gtc gtt 931 Arg Ser Phe Leu Gly Lys Leu Gln Asp Arg Arg Gly Asp Asn Val Val 265 270 275 att tac ttt gga ttg ttc ttc ttc gtt att tcc ttg acg att ttg tcc 979 He Tyr Phe Gly Leu Phe Phe Phe Val He Ser Leu Thr He Leu Ser 280 285 290 ttt gcc act tcc aac tgg cac gtt gtg 1006 Phe Ala Thr Ser Asn Trp His Val Val 295 300 < 210 > 184 < 211 > 302 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 184 Val Ser Val Ala Glu Glu Gly Lys Leu Phe Thr Pro Thr Phe Val Met 1 5 10 15 Gly Trp Phe Wing Asn Leu Phe Gln Phe Leu Val Phe Tyr Phe Leu He 20 25 30 Thr Thr Met Ala Leu Tyr Ala He Lys Glu Phe Gln Wing Ser Glu Val 35 40 45 Glu Wing Gly Phe Wing Being Ser He Val He Gly Wing Val Phe Ser 50 55 60 Arg Phe Phe Ser Gly Tyr He He Asp Arg Phe Gly Arg Arg Lys He 65 70 75 80 Val Leu He Ser Val Leu Val Thr Thr He Ala Cys Ala Leu Tyr Leu 85 90 95 Pro He Glu Ser Leu Pro Leu Leu Tyr Ala Asn Arg Phe Leu His Gly 100 105 110 Val Gly Tyr Ala Phe Ala Ala Thr Ala He Met Ala Ala Val Gln Glu 115 120 125 Leu He Pro Wing Ser Arg Arg Ser Glu Gly Thr Gly Tyr Leu Ala Leu 130 135 140 Gly Thr Thr Val Ser Wing Wing Leu Gly Pro Wing Leu Wing Leu Phe Val 145 150 155 160 Leu Gly Thr Phe Asp Tyr Asp Met Leu Phe He Val Val Leu Ala Thr 165 170 • 175 Ser Val He Ser Leu He Ala Val Val Phe Met Tyr Phe Lys Thr Ser 180 185 190 Asp Pro Glu Pro Ser Gly Glu Pro Wing Lys Phe Ser 'Phe Lys Ser He 195 200. 205 Met Asn Pro Lys He He Pro He Gly He Phe He Leu Leu He Cys 210 215 220 Phe Ala Tyr Ser Gly Val He Ala Tyr He Asn Ala Phe Ala Glu Glu 225 230 235 240 Arg Asp Leu He Thr Gly Wing Gly Leu Phe Phe He Wing Tyr Wing Val 245 250 255 Being Met Phe Val Met Arg Being Phe Leu Gly Lys Leu Gln Asp Arg Arg 260 265 _ 270 Gly Asp Asn Val Val He Tyr Phe Gly Leu Phe Phe Phe Val He Ser 275 280 - 285 Leu Thr He Leu Ser Phe Wing Thr Ser Asn Trp His Val Val 290 295 300 < 210 > 185 < 211 > 568 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (568) < 223 > RXA00858 < 400 > 185 ttttgttttt cagatgcatg ttagatgcgt tgagggacaa gggtggggga gacctccggt 60 tcttaaattg tctaaccaag aaccggaggt tctttttgtc atg gaa gta aac tta 115 Met Glu Val Asn Leu 1 5 gcc aca tgg cta atc act atc gca gtg att gct ggc ttc ttc att ttc 163 Wing Thr Trp Leu He Thr He Ala Val He Wing Gly Phe Phe He Phe 10 15 20 gat ttc tat tcc cac gtc cgc acc cca cac gag ccc act atc aaa gaa 211 Asp Phe Tyr Ser His Val Arg Thr Pro His Glu Pro Thr He Lys Glu 25 30 35 tcc gca tgg tgg age ctc tcc tac gta gcc ctc gcc tgt gtt tcc ggc 259 Ser Wing Trp Trp Ser Leu Phe Tyr Val Wing Leu Wing Cys Val Phe Gly 40 45 50 gtg ttc ctc tgg ttt gct tg ggc gag cca ggt aac cca cac cag cag 307 Val Phe Leu Trp Phe Wing Trp Gly Glu Pro Gly Asn Pro His Gln His 55 60 65 ggc att gag ttc ttc acc ggt tac gtg aca gag aag gcg ttg agt gtt 355 Gly He Glu Phe Phe Thr Gly Tyr Val Thr Glu Lys Ala Leu Ser Val 70 75 80 85 gat aac ctc ttc atc tcc gcg ctg atc ggt tct ttc aag att ect 403 Asp Asn Leu Phe He Phe Wing Leu He Met Gly Ser Phe Lys He Pr o 90 95 100 cgc aag tac cag cag aag gtt ctg ctc atc ggt atc gcg ctg gca ctg 451 Arg Lys Tyr Gln Gln Lys Val Leu Leu He Gly He Ala Leu Ala Leu 105 110. 115 gtc ttc cgc ctg gca ttc atc ctc gca ggt gct gca gtt atc gaa gcc 499 Val Phe Arg Leu Wing Phe He Leu Wing Gly Wing Wing Val He Glu Wing 120 125 130 tgg tcc gat gtc ttc tc tc atc tc tcc atc tgg ctg atc tac acc gct 547 Trp Ser Asp Val Phe Tyr He Phe Ser He Trp Leu He Tyr Thr Wing 135 140 145 gtg aag gct ect gtg cac gag 568 Val Lys Ala Pro Val His Glu 150 155 < 210 > 186 < 211 > 156 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 186 Met Glu Val Asn Leu Wing Thr Trp Leu He Thr He Wing Val He Wing 10 15 Gly Phe Phe He Phe Asp Phe Tyr Ser His Val Arg Thr Pro His Glu 20 25 * 30 Pro Thr He Lys Glu Ser Wing Trp Trp Ser Leu Phe Tyr Val Ala Leu 35 40 45 Wing Cys Val Phe Gly Val Phe Leu Trp Phe Wing Trp Gly Glu Pro Gly 50 55 60 Asn Pro His Gln His Gly He Glu Phe Phe Thr Gly Tyr Val Thr Glu 65 70 75 80 Lys Ala Leu Ser Val Asp Asn Leu Phe He Phe Wing Leu He Met Gly 85 90 95 Being Phe Lys He Pro Arg Lys Tyr Gln Gln Lys Val Leu Leu He Gly 100 105 110 He Ala Leu Ala Leu Val Phe Arg Leu Ala Phe He Leu Ala Gly Wing 115 120 125 Wing Val He Glu Wing Trp Ser Asp Val Phe Tyr He Phe Ser He Trp 130 135 140 Leu He Tyr Thr Wing Val Lys Wing Pro Val His Glu 145 150 155 < 210 > 187 < 211 > 975 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (952) < 223 > RXA02305 < 400 > 187 tatgcgcgca ggtgtctact ggtgaegeag ccgacgacga ttattttgac gaagccaccg 60 caaacgatga cttcgatccc gaaaagtgga ggaacatgta atg cca gcc ttt gag 115 Met Pro Ala Phe Glu 1 5 gca atg cca gga atg ccg tat tgg atc gac ctg tcc acc tcg gac att 163 Met Wing Pro Gly Met Pro Tyr Trp He Asp Leu Ser Thr Ser Asp He 10 15 • 20 gca aaa tct gca cac ttc tac gaa aac gtt ctc ggc tgg gaa att gaa 211 Wing Lys Ser Wing His Phe Tyr Glu Asn Val Leu Gly Trp Glu He Glu 25 30 35 gaa gtc aac gat ggc tac cgc atg gct cgt ctg cag gga cta ctc gtg 259 Glu Val Asn Asp Gly Tyr Arg Met Wing Arg Leu Gln Gly Leu Pro Val 40 45 50 gca ggg ctg atc gat cag cgc ggt gaa tea age atc ccg gat acc tgg 307 Wing Gly Leu He Asp Gln Arg Gly Glu Ser Ser He Pro Asp Thr Trp 55 60 65 att acc tac tc c tc tc cc tc gc tc gc tc gc tc gc tc gc tc atc atc 355 He Thr Tyr Phe Leu Ser Tyr Asp Leu Asp Wing Thr Wing Lys Lys He 70 75 80 85 gca gaa ctg ggt gga cga att ctg gcc gag cca act gac gtg cac ttg 403 Wing Glu Leu Gly Gly Arg He Leu Wing Glu Pro Thr Asp Val H is Leu 90 95 100 gga cgc atg atc cta gct gtt gat act gcc gcc gca gt ctg gt gt gtc gt 451 Gly Arg Met He Leu Wing Val Asp Thr Wing Gly Ala Leu Phe Gly Val 105 110 115 att gag cca ggc age gag gaa tea ttc gtc gct gct ggt gaa cca ggc 499 He Glu Pro Gly Ser Glu Glu Ser Phe Val Ala Wing Gly Glu Pro Gly 120 125 130 aca tcc gtg tgg cat gaa ctc acc act gtc tcc aaa tat tcc gaa gct 547 Thr Ser Val Trp His Glu Leu Thr Thr Val Ser Lys Tyr Ser Glu Wing 135 140 145 atc gat ttc tac ggt gag ctg ttc ttc ac tct gaa atg gct 595 He Asp Phe Tyr Gly Glu Leu Phe Thr Trp Thr Thr Ser Glu Met Wing 150 155 160 • 165 agt gct gaa gac gat agt ttc cgc tac acc acc gca ttg gct gac ggt 643 Ser Wing Glu Asp Asp Ser Phe Arg Tyr Thr Thr Wing Leu Wing Asp Gly 170 175 180 tcc gcc ttt gct gga att ttt gat gcc aaa ggc cac ttc cca ect cag 691 Be Wing Phe Wing Gly He Phe Asp Wing Lys Gly His Phe Pro Pro Gln 185 190 195 gtt cca age ttc tgg cag tcc tac ctt ggc gtg ctc aac gcc gat gat 739 Val Pro Ser Phe Trp Gln Ser Tyr Leu Gly Goes l Leu Asn Wing Asp Asp 200 205 210 gct gca gcg aag gcc aag gaa ttt ggt ggc gat gtt att cgt aag cca 787 Wing Wing Lys Wing Lys Glu Phe Gly Gly Asp Val He Arg Lys Pro 215 220 225 tgg gac tea gaa ttt ggc cgc atg gtt ctc atc tct gat tcc act ggt 835 Trp Asp Ser Glu Phe Gly Arg Met Val Leu He Ser Asp Ser Thr Gly 230 235 240 245 gcc aca att acc ttg tgt gaa gta gag gaa tac gtc gag gaa gca gca 883 Wing Thr He Thr Leu Cys Glu Val Glu Glu Tyr Val Glu Glu Ala Ala 250 255 260 gaa ggc gat gat ctc ttc gac atc gat ctc agt gct ttc gaa gag cag 931 Glu Gly Asp Asp Leu Phe Asp He Asp Leu Ser Ala Phe Glu Glu Gln 265 270 275 ttc cgc aag caa gaa gga cag taatcctaca gcgccatgga gga 975 Phe Arg Lys Gln Glu Gly Gln 280 < 210 > 188 < 211 > 284 < 212 > PRT • < 213 > Corynebacterium glutamicum < 400 > 188 Met Pro Wing Phe Glu Wing Met Pro Gly Met Pro Tyr Trp He Asp Leu 1 5 10 15 Ser Thr Ser Asp He Wing Lys Ser Wing His Phe Tyr Glu Asn Val Leu 20 25 30 Gly Trp Glu He Glu Glu Val Asn Asp Gly Tyr Arg Met Wing Arg Leu 35 40 45 10 Gln Gly Leu Pro Val Wing Gly Leu He Asp Gln Arg Gly Glu Being 50 55 _ 60 • He Pro Asp Thr Trp He Thr Tyr Phe Leu Ser Tyr Asp Leu Asp Wing 65 70 75 80 Thr Ala Lys Lys He Wing Glu Leu Gly Gly Arg He Leu Wing Glu Pro 85 90 95 Thr Asp Val His Leu Gly Arg Met He Leu Wing Val Asp Thr Wing Gly 100 105 110 15 Wing Leu Phe Gly Val He Glu Pro Gly Ser Glu Glu Ser Phe Val Wing 115 120 125 Wing Gly Glu Pro Gly Thr Ser Val Trp His Glu Leu Thr Thr Val Ser 130 135 140 Lys Tyr Ser Glu Wing He Asp Phe Tyr Gly Glu Leu Phe Thr Trp Thr 145 150 155 160 • Thr Ser Glu Met Wing Wing Wing Glu Asp Asp Ser Phe Arg Tyr Thr Thr 165 170 175 20 Wing Leu Wing Asp Gly Wing Wing Phe Wing Wing He Phe Asp Wing Lys Gly 180 185 190 His Phe Pro Pro Gln Val Pro Ser Phe Trp Gln Ser Tyr Leu Gl and Val 195 200 205 Leu Asn Wing Asp Asp Wing Wing Wing Lys Wing Lys Glu Phe Gly Gly Asp 210 215 220 Val He Arg Lys Pro Trp Asp Ser Glu Phe Gly Arg Met Val Leu He 225 230 235 240 25 Ser Asp Ser Thr Gly Wing Thr He Thr Leu Cys Glu Val Glu Glu Tyr 245 250 255 Val Glu Glu Ala Wing Glu Gly Asp Asp Leu Phe Asp He Asp Leu Ser 260 265 270 Wing Phe Glu Glu Gln Phe Arg Lys Gln Glu Gly Gln 275 280 < 210 > 189 < 211 > 948 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (925) < 223 > RXA00084 < 400 > 189 tcacccttgt cgataccagc tactgggtat ctggcgtcgg tccacttggc ggcagcaaag 60 tcttggaaga catcgatgcc ttcctcgacg cacagcaata atg tcc aca gct ctc 115 Met Ser Thr Ala Leu 1 5 ccc gat cag ctc aag tgg gaa tac agt gcc ttc ccc gtg cag atc tcg 163 Pro Asp Gln Leu Lys Trp Glu Tyr Be Ala Phe Pro Val Gln Be Ser 10 15 20 cag aag caa cgg ctt agt ccc ggc ttc atg cgg atc acc gtc act ggt 211 Gln Lys Gln Arg Leu Ser Pro Gly Phe Met Arg lie Thr Val Thr Gly 25 30 '35 gac aag ctc cga ttc ttt ggc cag tgg ggt ttg gac caa cgc atc aaa 259 Asp Lys Leu Arg Phe Phe Gly Gln Trp Gly Leu Asp Gln Arg He Lys 40 45 50 ctg atc att cca age ccg gct ggg aac atc cca gát ttc gga att ctc 307 Leu He He Pro Pro Pro Wing Gly Asn He Pro Asp Phe Gly He Leu 55 60 65 gac gaa ccc act ccc cca ccg aca acg tgg cct ect cct gct aag tct 355 Asp Glu Pro Pro Thr Pro Pro Thr Thr Trp Leu Pro Arg Wing Lys Ser 70 75 80"85 ttt cca gcg gac caa cga ccg atc ttg cgc acc tac ac cc tct gcg 403 Phe Pro Wing Asp Gln Arg Pro He Leu Arg Thr Tyr Thr Pro Be Wing 90 95 100 gtc cga ccc gaa cta tgc gaa gta gac att gat atc tat ctt cac aac 451 Val Arg Pro Glu Leu Cys Glu Val Asp He Asp He Tyr Leu His Asn 105 110 115 ect tcg gga cca gta tcc aga tgg gca aag aac tgc agt gtt gac gat 499 Pro Ser Gly Pro Val Ser Arg Trp Ala Lys Asn Cys Ser Val Asp Asp 120 125 130 gaa cta atc atc acc ggc ect gac gta cgc gca gga gaa acc ggc tac 547 Glu Leu He He Thr Gly Pro Asp Val Arg Wing Gly Glu Thr Gly Tyr 135 140 145 gga atc acc tat cat ccg act tct gcg atc gat cgc ctc ctc ctc atc 595 Gly He Thr Tyr His Pro Thr Ser Wing Asp Arg Leu Cys Leu He 150 155 160 165 ggc gat tgt gca tea gct ccc gcg atc gca aat atc gtc aat caa tea 643 Gly Asp Cys Wing Wing Pro Wing Wing Wing Asn lie Val Asn Gln Ser 170 175 aaa gta ect act acg gtt ttc ctc cac gta gac age cta gaa gat gat 691 Lys Val Pro Thr Thr Val Phe Leu His Val Asp Ser Leu Glu Asp Asp 185 190 195 gta ttg atc gcc gat age tcc acc aag ctc act ttc gaa gac atc gac 739 Val Leu He Ala Asp Ser Ser Thr Lys Leu Thr Phe G lu Asp He Asp 200 205 210 gct tac aaa gca aag gtc ttc caa tgg gct tea gcc aat gca gca gat 787 Wing Tyr Lys Wing Lys Val Phe Gln Trp Wing Being Wing Asn Wing Wing Asp 215 220 225 ect tea gta cac ttc tgg atc gcc ggt gaa act age atg gtg cgc ttc 835 Pro Ser Val His Phe Trp He Wing Gly Glu Thr Ser Met Val Arg Phe 230 235 240 245 att cgc aaa gaa cta atc aac age tac cga gtt gat tcc tea cga atc 883 He Arg Lys Glu Leu He Asn Ser Tyr Arg Val Asp Ser Ser Arg He 250 255 260 act ttc ctc ggc tac tgg aaa tac ggc cga cga acc gta gac 925 Thr Phe Leu Gly Tyr Trp Lys Tyr Gly Arg Arg Thr Val Asp 265 270 275 tagctttcag attcagaccc cag 948 < 210 > 190 < 211 > 275 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 190 Met Ser Thr Wing Leu Pro Asp Gln Leu Lys Trp Glu Tyr Ser Wing Phe 1 5 10 15 Pro Val Gln He Ser Gln Lys Gln Arg Leu Ser Pro Gly Phe Met Arg 20 25 30 He Thr Val Thr Gly Asp Lys Leu Arg Phe Phe Gly Gln Trp Gly Leu 35 40 45 sp Gln Arg He Lys Leu He He Pro Pro Pro Wing Gly Asn He Pro 50 55 60 Asp Phe Gly He Leu Asp Glu Pro Pro Thr Pro Pro Thr Thr Trp Leu 65 70 75 • 80 Pro Arg Ala Lys Ser Phe Pro Wing Asp Gln Arg Pro He Leu Arg Thr 85 90 95 Tyr Thr Pro Ser Wing Val Arg Pro Glu Leu Cys -Glu Val Asp He Asp 100 105 110 He Tyr Leu His Asn Pro Ser Gly Pro Val Ser Arg Trp Wing Lys Asn 115 120 125 Cys Ser Val Asp Asp Glu Leu He He Thr Gly Pro Asp Val Arg Wing 130 135 140 Gly Glu Thr Gly Tyr Gly He Thr Tyr His Pro Thr Ser Wing Asp 145 150 155 160 Arg Leu Cys Leu He Gly Asp Cys Wing Being Wing Pro Wing He Wing Asn 165 170 175 He Val Asn Gln Ser Lys Val Pro Thr Thr Val Phe Leu His Val Asp 180 185 190 Ser Leu Glu Asp Asp Val Leu He Wing Asp Ser Ser Thr Lys Leu Thr 195 200 205 Phe Glu Asp He Asp Wing Tyr Lys Wing Lys Val Phe Gln Trp Wing Ser 210 215 220 Wing Asn Wing Wing Asp Pro Ser Val His Phe Trp He Wing Gly Glu Thr 225 230 235 240 Being Met Val Arg Phe He Arg Lys Glu Leu He Asn Being Tyr Arg Val 245 250 '255 Asp Ser Being Arg He Thr Phe Leu Gly Tyr Trp Lys Tyr Gly Arg Arg 260 265 270 Thr Val Asp 275 < 210 > 191 < 211 > 468 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (445) < 223 > RXA00843 < 400 > 191 gccctgatgc gaaaccggcg ccaacaatga tgccgacgaa ggcaaatgcc actcttagga 60 tttgaataat catggaacaa accttagtag gctcaacgtt atg aaa gtc acg att 115 Met Lys Val Thr He 1 5 ttc cat aat ccg cgt tgt tcc aca tcc aga aat acc ctc gct tac ctc 163 Phe His Asn Pro Arg Cys Ser Thr Being Arg Asn Thr Leu Wing Tyr Leu 10 15 20 cgc gac aag gac att gag ect gaa att gtt cag tat ctc aaa gac acg 211 Arg Asp Lys Asp He Glu Pro Glu He Val Gln Tyr Leu Lys Asp Thr 25 30 35 ccc acc gct tcc gag ctc aaa gaa cta ttc aat acg ctg gga att cca 259 Pro Thr Wing Ser Glu Leu Lys Glu Leu Phe Asn Thr Leu Gly He Pro 40 45 50 gtc cac gac ggc atc aga acc gc gac gct gac tac aca gaa ctg ggc 307 Val His Asp Gly He Arg Thr Arg Glu Wing Glu Tyr Thr Glu Leu Gly 55 60 65 ctg tea cca gaa aca ect gaa act gag ctt atc gac gcc atc gtt gcc 355 Leu Ser Pro Glu Thr Pro Glu Thr Glu Leu He Asp Ala He Val Ala 70 75 80 85 cat ccc agg ctc ctt cag cgt ccg atc gtg gtg acg gcc aaa ggc gcg 403 His Pro Arg Leu Leu Gln Arg Pro He Val Val Thr Ala Lys Gly Wing 90 95 100 cgc att gcg cgc ccc aaa atc gac gtc att gac age atc ttg 445 Arg He Wing Arg Pro Lys He Asp Val He Asp Ser He Leu 105 110 115 tgacaacatt ttgtagagca acc 468 < 210 > 192 < 211 > 115 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 192 Met Lys Val Thr He Phe His Asn Pro Arg Cys Ser Thr Ser Arg Asn 1 5 10 15 Thr Leu Wing Tyr Leu Arg Asp Lys Asp He Glu Pro Glu He Val Gln 20 25 30 Tyr Leu Lys Asp Thr Pro Thr Wing Ser Glu Leu Lys Glu Leu Phe Asn 35 40 45 Thr Leu Gly He Pro Val His Asp Gly He Arg Thr Arg Glu Wing Glu 50 55 60 Tyr Thr Glu Leu Gly Leu Ser Pro Glu Thr Pro Glu Thr Glu Leu He 65 70 75 80 Asp Ala He Val Ala Pro Arg Leu Leu Gln Arg Pro He Val Val 85 90 95 Thr Ala Lys Gly Ala Arg He Ala Arg Pro Lys He Asp Val He Asp 100 105 110 Ser He Leu 115 < 210 > 193 < 211 > 432 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (409) < 223 > RXA01052 < 400 > 193 tatggccaac cctaggggga tggcctgtgt gttcactgtt aggtttcctc aaaatcttta 60 acgaacaacg aagagcttgc ccgagagtat cttgggtcgc atg gac aca aaa tta 115 Met Asp Thr Lys Leu 1 5 ggc gct gaa ttg ggt act gaa ttt gat ctc att gtt gtt ggt ttc ggc 163 Gly Ala Glu Leu Gly Thr Glu Phe Asp Leu He Val Val Gly Phe Gly 10 15 20 aaa gca ggc aag act atc gcg atg aaa cgc tcg gca gcg ggg gat aag 211 Lys Ala Gly Lys Thr He Ala Met Lys Arg Ser Ala Ala Gly Asp Lys 25 30 35 gtc gca ctg atc gag cag agt cca cag atg tat ggc ggt acc tgc atc 259 Val Ala Leu He Glu Gln Ser Pro Gln Met Tyr Gly Gly Thr Cys He 40 45 50 aat gta ggt tgc atc ccc acg aag tag a ttg ttt ttt gag act gca acg 307 Asn Val Gly Cys He Pro Thr Lys Lys Leu Leu Phe Glu Thr Wing Thr 55 60 65 ggc aag gat ttc ccg gat gcg gtt gtg gcg cgt gat cag ttg att ggc 355 Gly Lys Asp Phe Pro Asp Ala Val Val Ala Arg Asp Gln Leu He Gly 70 75 80 85 aag ctg aat gcc aag aat ctt gcg atg gcc aca gac aag ggt gtc acc 403 Lys Leu Asn Ala Lys Asn Leu Ala Met Ala Thr Asp Lys Gly Val Thr 90 95 100 cgt cat tgatggaaaa gctacgttta cag 432 Arg His < 210 > 194 < 211 > 103 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 194 Met Asp Thr Lys Leu Gly Wing Glu Leu Gly Thr Glu Phe Asp Leu He 1 5 10 15 Val Val Gly Phe Gly Lys Wing Gly Lys Thr He Wing Met Lys Arg Ser 20 25 30 Wing Wing Gly Asp Lys Val Wing Leu He Glu Gln Ser Pro Gln Met Tyr 35 40 45 Gly Gly Thr Cys He Asn Val Gly Cys He Pro Thr Lys Lys Leu Leu 50 55 60 Phe Glu Thr Wing Thr Gly Lys Asp Phe Pro Asp Wing Val Val Wing Arg 65 70 75 80 Asp Gln Leu He Gly Lys Leu Asn Wing Lys Asn Leu Wing Met Wing Thr 85 90 95 Asp Lys Gly Val Thr Arg His 100 < 210 > 195 < 211 > 543 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (520) < 223 > RXA01053 < 400 > 195 ttgcgatggc cacagacaag ggtgtcaccc gtcattgatg gaaaagctac gtttacagct 60 agccacgaaa tcacaagtaa cttcaggtag tgacactctt gtg ctg tat cgc cca 115 Val Leu Tyr Ala Pro 1 5 acg att gtg atc aac acg ggc tcc acg ccg gtc atc ccc aat gtc cca 163 Thr He Val He Asn Thr Gly Ser Thr Pro Val He Pro Asn Val Pro 10 15 20 ggc acc gac aat ccg cat gtt ttt gat tcc act ggc att cag cac att 211 Gly Thr Asp Asn Pro His Val Phe Asp Ser Thr Gly He Gln His He 25 30 35 tcg ccc ctg ccg aag cac ctc gcg atc atc ggc ggt gcc ccc atc ggt 259 Pro Pro Leu Pro Lys His Leu Wing He Gly Gly Gly Pro He Gly 40 45 50 ttg gaa ttt gcc acg ctt ttc agt gga caa ggc tcc aaa gtc acc atc 307 Leu Glu Phe Wing Thr Leu Phe Ser Gly Gln Gly Ser Lys Val Thr He 55 60 65 atc gac cgt ggt gaa ttg ccg ctg aaa aat ttc gac agg gaa gta gcg 355 He Asp Arg Gly Glu Leu Pro Leu Lys Asn Phe Asp Arg Glu Val Ala 70 75 80 85 • gag ctg gcc aaa acc gac ctg gag gcc cgc gga atc acc ttc ctc aac 403 5 Glu Leu Wing Lys Thr Asp Leu Glu Wing Arg Gly He Thr Phe Leu Asn 90 95 100 aac gct gaa ctc acc gga ttc age ggt gac ctc acc atc gcg ctc aaa 451 Asn Wing Glu Leu Thr Gly Phe Ser Gly Asp Leu Thr He Ala Leu Lys 105 110 115 gac cac gac ctc ctc gcc gac gcc gca ctt ttt gca tcg gcc gac gcc 499 Asp His Asp Leu Leu Wing Asp Wing Wing Leu Phe Wing Wing Wing Asp Wing 120 125 130 cgg cc cga cgg cgg ect tgaacaggcg ggcatcaaaa cag 543 10 Arg His Arg Arg Ala Arg Pro 135 140 • < 210 > 196 < 211 > 140 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 196 Val Leu Tyr Ala Pro Thr He Val He Asn Thr Gly Ser Thr Pro Val 1 5 10 15 15 He Pro Asn Val Pro Gly Thr Asp Asn Pro His Val Phe Asp Ser Thr 20 25 30 Gly He Gln His He Ser Pro Leu Pro Lys His Leu Wing He He Gly 35 40 45 Gly Gly Pro He Gly Leu Glu Phe Wing Thr Leu Phe Ser Gly Gln Gly • 50 55 60 Ser Lys Val Thr He He Asp Arg Gly Glu Leu Pro Leu Lys Asn Phe 65 70 75 80 20 Asp Arg Glu Val Wing Glu Leu Wing Lys Thr Asp Leu Glu Wing Arg Gly 85 90 95 He Thr Phe Leu Asn Asn Wing Glu Leu Thr Gly Phe Ser Gly Asp Leu 100 105 110 Thr He Wing Leu Lys Asp His Asp Leu Leu Wing Asp Ala Ala Leu Phe 115 120 125 Ala Be Ala Asp Ala Arg His Arg Arg Ala Arg Pro 130 135 140 25 < 210 > 197 < 211 > 612 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (589) < 223 > RXA01054 < 400 > 197 gacctcctcg ccgacgccgc actttttgca tcggccgacg cccggcaccg acgggctcgg 60 ccttgaacag gcgggcatca aaacaggcac gcgtggggag gtg ctt gtc gac gcc 115 Val Leu Val Asp Ala • 1 5 cac ctc cgg acc aac atc gac ggc atc tcc gct gta ggt gat gtc aat 163 His Leu Arg Thr Asn He Asp Gly He Phe Wing Val Gly Asp Val Asn 10 15 20 ggc ggc ccg cag ttt acc tac gtg tcc tac gat gac cac cgc att gtg 211 Gly Gly Pro Gln Phe Thr Tyr Val Ser Tyr Asp Asp His Arg He Val 25 30. 35 ctg gat caa cta gcc gga ggt aag aaa tcc att gca cac cga ctg 259 Leu Asp Gln Leu Wing Gly Thr Gly Lys Lys Ser He Wing His Arg Leu 40 45 • 50 atc ccc acc acc ac ttc atc gaa ccg ccg tta tcc acc atc ggt gac 307 He Pro Thr Thr Thr Phe He Glu Pro Pro Leu Ser Thr He Gly Asp 55 60 65 aac act gaa ggg gaa aat gtg gtg gtg aaa aag gcc ttg att gca gat 355 Asn Thr Glu Gly Glu Asn Val Val Val Lys Lys Wing Leu He Wing Asp 70 75 80 85 atg ccg atc gtt ccc cga cca gag att att aac caa ect cac ggt atg 403 Met Pro Pro Val Pro Ar Glu He He Asn Gln Pro His Gly Met 90 95 100 gtg aag ttt ttc gtc gac aag caa tct gat gcg ctg ctc ggc gcg acc 451 Val Lys Phe Phe Val Asp Lys Gln Ser Asp Wing Leu Leu Gly Wing Thr 105 110 115 ttg tac tgc gcc gac tcc cag gag ctc atc aac ac gtg gcg ctt gcc 499 Leu Tyr Cys Wing Asp Ser Gln Glu Leu He Asn Thr Val Wing Leu Wing 120 125 130 atg cgg cat ggc gtc gcc ccc acc gcc gac gc gc gc atc tac acc 547 Met Arg His Gly Val Thr Wing Ser Glu Leu Gly Asp Gly He Tyr Thr 135 140 14 5 cac ccc gcc acc tcg gag atc tc aac caa tta ttg ggc agt 589 His Pro Wing Thr Ser Glu He Phe Asn Gln Leu Leu Gly Ser 150 155 160 taacgcagcg gatcgaacgg ctt 612 < 210 > 198 < 211 > 163 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 198 Val Leu Val Asp Ala His Leu Arg Thr Asn He Asp Gly He Phe Wing 1 5 10 15 Val Gly Asp Val Asn Gly Gly Pro Gln Phe Thr Tyr Val Ser Tyr Asp 20 25 30 Asp His Arg He Val Leu Asp Gln Leu Ala Gly Thr Gly Lys Lys Ser 35 40 45 He Wings His Arg Leu He Thr Pro Thr Thr Thr Phe He Glu Pro Pro Leu 50 55 60 Ser Thr He Gly Asp Asn Thr Glu Gly Glu Asn Val Val Val Lys Lys 65 70 75 80 Wing Leu He Wing Asp Met Pro He Val Pro Arg Pro Glu He He Asn 85 90 95 Gln Pro His Gly Met Val Lys Phe Phe Val Asp Lys Gln Ser Asp Wing 100 105 110 Leu Leu Gly Wing Thr Leu Tyr Cys Wing Asp Ser Gln Glu Leu He Asn 115 120 125 Thr Val Ala Leu Ala Met Arg His Gly Val Thr Ala Ser Glu Leu Gly 130 135 140 Asp Gly He Tyr Thr His Pro Wing Thr Ser Glu He Phe Asn Gln Leu 145 150 155 160 Leu Gly Ser < 210 > 199 < 211 > 561 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (538) < 223 > RXN03123 < 400 > 199 agctctacca acgcgcctac accttgacca acgtggatgc cgatgccggt acctttgacc 60 tggcttttgt gctgcacgag ccgctggggc ccgcctcggc gtg gcc gac gcg ctg 115 Val Gly Asp Ala Leu 1 • 5 cgc ggc cgg gga aag ect gaa gtc atg cgc tac cca gga att ccg ttc 163 Arg Gly Arg Gly Lys Pro Glu Val Met Arg Tyr Pro Gly He Pro Phe 10 15 20 5 gcc atc cca gat cca gcg ccg cgt ggc ttc ctt ttc tta ggc gat ctc 211 Wing Pro Pro Asp Pro Pro Wing Arg Gly Phe Leu Phe Leu Gly Asp Leu 25 30 35 acc tct tac cca gcg atc tgc tcg att ctg gag acc ttg gac ggt gaa 259 Thr Ser Tyr Pro Ala He Cys Ser He Leu Glu Thr Leu Asp Gly 40 45 50 atc ect gcg gcg gcg tat ctt atc gcc cac gat cca ctt gat tac acc 307 He Pro Wing Thr Wing Tyr Leu He Wing His Asp Pro Leu Asp Tyr Thr 55 60 65 10 ttc gat ttt ccc cag ggc gag cac atc acc gcg cag tgg att tcc aac 355 Phe Asp Phe Pro Gln Gly Glu His He Thr Ala Gln Trp He Ser Asn 70 75 80 85 • gaa caa tcc ttc att gat cac atc gct gac acg gat tac acc gat ttt 403 Glu Gln Ser Phe He Asp His He Ala Asp Thr Asp Tyr Thr Asp Phe 90 95 100 tat acc tgg atc ggc gcg gaa tcc tcc gaa acc cgt gcg gcc aag aag 451 Tyr Thr Trp He Gly Wing Glu Ser Ser Glu Thr Arg Wing Wing Lys 105 110 115 15 cat ctg cag acc cacc gcc ggc atg ccc aag acg cac atg aac gcg caa 499 His Leu Gln Thr His Wing Gly Met Pro Lys Thr His Met Asn Wing Gln 120 125 130 ggt tat tgg aac aag ggc aga gcc atg ggt aaa age aat taaaagattt 548 Gly Tyr Trp Asn Lys Gly Arg Wing Met Gly Lys Ser Asn 135 140 145 ttgcttatcg acg 561 • < 210 > 200 20 < 211 > 146 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 200 Val Gly Asp Ala Leu Arg Gly Arg Gly Lys Pro Glu Val Met Arg Tyr 1 5 10 15 Pro Gly He Pro Phe Ala He Pro Asp Pro Ala Pro Arg Gly Phe Leu 20 25 30 Phe Leu Gly Asp Leu Thr Ser Tyr Pro Ala He Cys Ser He Leu Glu 25 35 40 45 Thr Leu Asp Gly Glu He Pro Wing Thr Wing Tyr Leu He Wing His Asp 50 55 60 Pro Leu Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His He Thr Wing 65 70 75 80 Gln Trp He Ser Asn Glu Gln Ser Phe He Asp His He Wing Asp Thr 85 90 95 • Asp Tyr Thr Asp Phe Tyr Thr Trp He Gly Wing Glu Ser Ser Glu Thr 100 105 110 Arg Wing Wing Lys Lys His Leu Gln Thr His Wing Gly Met Pro Lys Thr 115 120 125 His Met Asn Wing Gln Gly Tyr Trp Asn Lys Gly Arg Wing Met Gly Lys 130 135 140 Ser Asn 10 145 < 210 > 201 • < 211 > 736 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (736) < 223 > FRXA00993 15 < 400 > 201 gctgagctag tgcttttgcg acacacctct tgcgaatgtt gattaggtta ggcaagccat 60 atttacaggg tgttgtgaaa gcataaggga gcaaggaaac atg ggc aag gga ttt 115 Met Gly Lys Gly Phe 1 5 acc ggc gct att ttg acc gtc atg ggc gtg aaa tcg cat atc gcc acc 163 Thr Gly Ala He Leu Thr Val Met Gly Val Lys Ser His He Wing Thr 10 15 20 20 acc acg gga aaa acc gtg atc aat gac cgc atg gtg acc att cat ttt 211 Thr Thr Gly Lys Thr Val He Asn Asp Arg Met Val Thr He His Phe 25 30 35 cat tcc gag acg ctg ctc aac acg gaa ggt gaa gtc ccc ggc gat tgg 259 His Ser Glu Thr Leu Leu Asn Thr Glu Glu Glu Val Pro Gly Asp Trp 40 45 50 ctg cgt ctg tgg ctg ccg cac gag age cga ect gga aag ctc tac a cca 307 Leu Arg Leu Trp Phe Pro His Glu Ser Arg Pro Gly Lys Leu Tyr Gln 55 60 65 25 cgc gcc tac acc ttg acc aac gtg gat gcc gat gcc ggt acc ttt gac 355 Arg Wing Tyr Thr Leu Thr Asn Val Asp Wing Asp Wing Gly Thr Phe Asp 70 75 • 80 85 ctg gct ttt gtg ctg cac gag ccg ctg ggg ccc gcc tcg gcg tgg gcg 403 Leu Wing Phe Val Leu His Glu Pro Leu Gly Pro Wing Wing Trp Wing 90 95 100 acg cgc tgc gag gcc ggg gaa age ctg gaa gtc att cgc tac cca gga 451 Thr Arg Cys Glu Wing Gly Glu Ser Leu Glu Val Met Arg Tyr Pro Gly 105 110 115 att ccg ttc gcc atc cca gat cca gcg ccg cgt ggc ttc ctt ttc cta 499 He Pro Phe Wing Pro Pro Asp Pro Wing Pro Arg Gly Phe Leu Phe Leu 120 125 • 130 ggc gat ctc acc tct tac cca g cg atc tgc tcg att ctg gag acc ttg 547 Gly Asp Leu Thr Ser Tyr Pro Wing He Cys Ser He Leu Glu Thr Leu 135 140 145 gac ggt gaa atc ect gcg acc gcg tat ctt atc gcc cac gat cca 596 Asp Gly Glu He Pro Ala Thr Ala Tyr Leu He Ala His Asp Pro Leu 150 155 160 165 gat tac acc ttc gat ttt ccc cag ggc gag cac atc acc gcg cagg tgg 643 Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His He Thr Ala Gln Trp 170 175 180 att tcc aac gaa caa tcc ttc att gat cac atc gct gac acg gat tac 691 He Ser Asn Glu Gln Ser Phe He Asp His He Wing Asp Thr Asp Tyr 185 190 195 tc tt tat ttat tc tc tc tc t acc cgt 736 Thr Asp Phe Tyr Thr Trp He Gly Wing Glu Ser Ser Glu Thr Arg 200 205 210 < 210 > 202 < 211 > 212 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 202 Met Gly Lys Gly Phe Thr Gly Wing He Leu Thr Val Met Gly Val Lys 1 5 10 15 Ser His He Wing Thr Thr Thr Gly Lys Thr Val He Asn Asp Arg Met 20 25 30 Val Thr He His Phe His Ser Glu Thr Leu Leu Asn Thr Glu Gly Glu 35 40 45 Val Pro Gly Asp Trp Leu Arg Leu Trp Phe Pro His Glu Ser Arg Pro 50 55 60 Gly Lys Leu Tyr Gln Arg A to Tyr Thr Leu Thr Asn Val Asp Wing Asp 65 70 75 80 Wing Gly Thr Phe Asp Leu Wing Phe Val Leu His Glu Pro Leu Gly Pro 85 90 95 Wing Be Wing Trp Wing Thr Arg Cys Glu Wing Gly Glu Ser Leu Glu Val 100 105 110 Met Arg Tyr Pro Gly He Pro Phe Wing He Pro Asp Pro Wing Pro Arg 115 120 125 Gly Phe Leu Phe Leu Gly Asp Leu Thr Ser Tyr Pro Wing He Cys Ser 130 135 140 He Leu Glu Thr Leu Asp Gly Glu He Pro Wing Thr Wing Tyr Leu He 145 150 155 160 Ala His Asp Pro Leu Asp Tyr Thr Phe Asp Phe Pro Gln Gly Glu His 165 170 175 He Thr Wing Gln Trp He Ser Asn Glu Gln Ser Pñe He Asp His He 180 185 '190 Wing Asp Thr Asp Tyr Thr Asp Phe Tyr Thr Trp He Gly Ala Glu Ser 195 200 205 Ser Glu Thr Arg 210 < 210 > 203 < 211 > 732 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (709) < 223 > RXA01051 < 400 > 203 tgcgacccaa gatactctcg ggcaagctct tcgttgttcg ttaaagattt tgaggaaacc 60 taacagtgaa cacacaggcc atccccctag ggttggccat atg tea acc att cac 115 Met Ser Thr He His 1 5 gcc tcc gga atc cag gct cca caa gtg cca cac ggt tcc cac cat gcc 163 Wing Ser Gly He Gln Wing Pro Gln Val Pro His Gly Ser His His Wing 10 15: 20 ccg cca caa aag gac gaa tea gtg aag aag age ttc aat gcc tct tct 211 Pro Pro Gln Lys Asp Glu Ser Val Lys Lys Ser Phe Asn Ala Ser Ser 25 30 35 tta ctg ttc gcg ttt tcc tcc ggc gtg tac ctg gtg ctg ctt gtg atg 259 Leu Leu Phe Wing Phe Ser Phe Gly Val Tyr Leu Val- Leu Leu Val Met 40 45 50 atg aca ctt ctt aaa agt cgc ctt tct tta ggc gga ctg tgg aac here 307 Met Thr Leu Leu Lys Ser Arg Leu Ser Leu Gly Gly Leu Trp Asn Thr 55 60 - * 65 gaa gca cac caa tac aga tcc atc gac tta gag ctt tcc aac ggc ttt 355 Glu Wing His Gln Tyr Arg Ser He Asp Leu Glu Leu Phe Asn Gly Phe 70 75 80 • '85 5 gct gat cca cca att tgg tgg ggg ect tgg acc aac act ttt ggc aac 403 • Wing Asp Pro Pro He Trp Trp Gly Pro Trp Thr Asn Thr Phe Gly Asn 90 95 100 atc gca ctg ttc atg cca ttt ggg ttt ttc ctg tac aaa atg ctc cgt 451 He Ala Leu Phe Met Pro Phe Gly Phe Phe Leu Tyr Lys Met Leu Arg 105 110 115 aga ttc aac cat cga ttc ccc ttc gta gaa acc atc ctg ttt gcc age 499 Arg Phe Asn His Arg Phe Pro Phe Val Glu Thr He Leu Phe Wing Ser 120 125 130 10 gtc acc age ctc agt atc gaa gtt ctg caa tgg gtg ttt gct att gga 547 Val Thr Ser Leu Ser He Glu Val Leu Gln Trp Val Phe Wing He Gly 135 140 145 • tat tea gat gtc gat gac ctg ttg ttt aat acg ate ggc gga ctc att 595 Tyr Ser Asp Val Asp Asu Leu Leu Phe Asn Thr He 'Gly Gly Leu He 150 155' - 160 165 gga gca tc gta gc gcg ctt gtc tcg ctt aaa tcc tcc aag gta gtc 643 Gly Ala Ser Val Ala Ala Leu Val Ser Leu Lys Ser Ser Lys Val Val 170 175 180 15 age gga atc atc ggc ggt tea cta tct gtg atg gcg atg atg atg 691 Ser Gly He Met Met Gly Gly Ser Leu Ser Val Met Ala Met Met Met 185 190 195 tat tea agt ttt atc gcc taga aggttt cagcagttcc gct 732 Tyr Ser Ser Phe He Wing 200 < 210 > 204 < 211 > 203 < 212 > PRT 20 < 213 > Corynebacterium glutamicum < 400 > 204 Met Ser Thr He His Wing Ser Gly He Gln Wing Pro Gln Val Pro His 1 5 10, 15 Gly Ser His His Wing Pro Pro Gln Lys Asp Glu Ser Val Lys Lys Ser 20 25 * 30 Phe Asn Wing Ser Ser Leu Leu Phe Wing Phe Ser Phe Gly Val Tyr Leu 35 40 45 25 Val Leu Leu Val Met Met Thr Leu Leu Lys Ser Arg Leu Ser Leu Gly • - - - J - 50 55 60 Gly Leu Trp Asn Thr Glu Wing His Gln Tyr Arg Ser He Asp Leu Glu 65 70 75 80 Leu Phe Asn Gly Phe Wing Asp Pro Pro He Trp Trp Gly Pro Trp Thr 85 90 95 Asn Thr Phe Gly Asn He Ala Leu Phe Met Pro Phe Gly Phe Phe Leu 100 105 110 Tyr Lys Met Leu Arg Arg Phe Asn His Arg Phe Pro Phe Val Glu Thr 115 120 125 He Leu Phe Wing Ser Val Thr Ser Leu Ser He Glu Val Leu Gln Trp 130 135 140 Val Phe Wing He Gly Tyr Ser Asp Val Asp Asp Leu Leu Phe Asn Thr 145 150 155 160 He Gly Gly Leu He Gly Ala Ser Val Ala Ala Ala Leu Val Ser Leu Lys 165 170 175 Ser Ser Lys Val Val Ser Gly He Met Met Gly Gly Ser Leu Ser Val 180 185 190 Met Wing Met Met Met Tyr Ser Ser Phe He Wing 195 200 < 210 > 205 < 211 > 1359 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1336) < 223 > RXN01873 < 400 > 205 ccgtcgttgc ccatggtcac agcctacatg cacaaagtga atcaaaaaca gctatttcta 60 acattttact aatatttgct gttggcgcat gatgaactcc atg age caa gca ata 115 Met Ser Gln Ala He 1 5 gat age aag gtc gag gca cac gaa ggc cac gaa ggc cac gaa ggc atc 163 Asp Ser Lys Val Glu Ala His Glu Gly His Glu Gly? Is Glu Gly He 10 15 20 gag cga gga aca cgc aat tac aag cgc gct gtg ttt gcg atg ctg gcc 211 Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val Phe Ala Met Leu Ala 25 30 35 gcc ggt ctt gct gct gtt aat gt ct tgc tgc acg cag gt ttg ctt 259 Ala Gly Leu Wing Wing Phe Asn Gly Leu Tyr Cys Thr Gln Wing Leu Leu 40 45 50 ccc acc atg ac gaga gag ttg gga att acg ccc act gag tcc gcg ctg 307 Pro Thr Met Thr Glu Glu Leu Gly He Thr Pro Thr Glu Be Ala Leu 55 60 65 acg gtg tcg gct acg act gga atg ttg gcg ctg tgt att gtt ccg gcg 355 Thr Val Ser Wing Thr Thr Gly Met Leu Wing Leu Cys He Val Pro Ala 70 75 80 85 tcg ata ctt tcg gag aaa ttt ggt cgc ggt cgg gtg ctg aca att tea 403 Ser He Leu Ser Glu Lys Phe Gly Arg Gly Arg Val Leu Thr He Ser 90 95 100 ctc acg ttg gcc atc atc gtg gga tta att ttg ccg ctt gtc ccc aat 451 Leu Thr Leu Ala He He Val Gly Leu He Leu Pro Leu Val Pro Asn 105 110 115 att act gct ctc atc ctg ctc aga ggt ctc caa ggt gcg ctg ctt gct 499 He Thr Ala Leu He Leu Leu Arg Gly Leu Gln Gly Ala Leu Leu Wing 10 120 125 130 ggc act cca gcg gtg gcg atg acc tgg ttg tct gag gaa att cac ccc 547 Gly Thr Pro Wing Val Ala Met Thr Trp Leu Ser Glu Glu He His Pro • 135 140 145 aag gat ggg cat gcg atg gga att tac atc gcg gga aat act gtc 595 Lys Asp He Gly His Wing Met Gly He Tyr He Wing Gly Asn Thr Val 150 155 160 165 ggc ggg ctc act gga cgt atg att ccg gcg gga cta ctt gaa gta act 643 Gly Gly Leu Thr Gly Arg Met He Pro Wing Gly Leu Leu Glu Val Thr 15 170 175 180 cat tgg caa aac gca ctg ctg gga agt tct atc gct gcg ctg atc ttc 691 His Trp Gln Asn Wing Leu Leu Gly Being Ser Wing Wing Ala Leu He Phe 185 190 • 195 ggc gta atc atg gtg gtg ttg ctt ccc aag cag cgg aaa ttc cag ccg 739 Gly Val He Met Val Val Leu Leu Pro Lys Gln Arg Lys Phe Gln Pro 200 205 210 • aaag aat atc aat ctg cgc cat gag att tcg atg gct gct cat tgg 787 Lys Asn He Asn Leu Arg His Glu He Ser Ala Ala Ala Ala His Trp 20 215 220 225 cgg aat ect cgt ttg gcg ttg ctt ttt ggt act gcg ttt tg ggc atg 835 Arg Asn Pro Arg Leu Wing Leu Leu Phe Gly Thr Wing Phe Leu Gly Met 230 235 240 245 ggt act ttt gtg tcg ctg tac aac tat ttg ggt ttc cgc atg att gat 883 Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly Phe Arg Met He Asp 250 255 •. 260 cag ttt ggg ctg agt gaa gtg ctg gtt ggt gcg gtg ttc atc tat 931 Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala Val Phe He Met Tyr 25 265 270. 275 ctg gcc ggg acc tgg agt tcc acc ggc gg gg gg tg agg gag aag 979 Leu Ala Gly Thr Trp Ser Ser Thr Gln Ala Gly Ala Leu Arg Glu Lys 280 285 290 'atc ggc aat ggg tea acg gtt att ttc ttg agt ptg acg atg atc gcg 1027 He Gly Asn Gly Ser Thr Val He Phe Leu Ser Leu Thr Met He Wing 295 300 305 • tcg atg gca ctg atg ggg att aat aat ttg tgg gtc acg ttg gtt gcc 1075 Ser Met Ala Leu Met Gly He Asn Asn Leu Trp Val Thr Leu Val Wing 310 315. 320 325 ctg ttt gtg ttt acc gcg gca ttt ttc gca ctg cat tcc agt gct tcg 1123 Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu His Ser Ser Ala Ser 330 335 340 gga tgg atc gga atc atc gca acg aag gat cgc gcg gaa gcc tcc age 1171 Gly Trp He Gly He He Wing Thr Lys Asp Arg Wing Glu Ala Ser Ser 345 350 355 10 atg tat ttg ttc tgt tat tac gtg gga tcc tcg gtg att ggt tgg gtt 1219 Met Tyr Leu Phe Cys Tyr Tyr Val Gly Ser Ser Val -He Gly Trp Val 360 365 370 • tct gga ttc gcg ttt acg cat ttg tcg tgg tg tcg gtc tgc tgc gtc 1267 Ser Gly Phe Wing Phe Thr His Leu Pro Trp Leu Wing Phe He Gly Trp 375 380 385 ttg att ctg ctt ctt tgc gga gtg ctg gcg att tgt gtg acg ctg gca 1315 Leu He Leu Leu Leu Cys Gly Val Leu Ala He Cys Val Thr Leu Wing 390 395 400 405 15 agg ctt gcc cgc aac gcc aat taatacgagt ttgtccgtgt tta 1359 Arg Leu Ala Arg Asn Ala Asn '' 410 < 210 > 206 < 211 > 412 • < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 206 20 Met Ser Gln Ala He Asp Ser Lys Val Glu Ala His Glu Gly His Glu 1 5 10 15 Gly His Glu Gly He Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val 20 25 30 Phe Ala Met Leu Ala Ala Gly Leu Ala Wing Phe Asn Gly Leu Tyr Cys 35 40 45 # Thr Gln Wing Leu Leu Pro Thr Met Thr Glu Glu Leu Gly He Thr Pro 50 55 60 25 Thr Glu Be Ala Leu Thr Val Ser Wing Thr Thr Gly Met Leu Ala Leu 65 70 75 80 Cys He Val Pro Wing Being He Leu Being Glu Lys Phe Gly Arg Gly Arg 85 90 95 Val Leu Thr He Ser Leu Thr Leu Wing He He Val Val Leu He Leu 100 105 110 Pro Leu Val Pro Asn He Thr Ala Leu He Leu Leu Arg Gly Leu Gln 115 120 125 Gly Ala Leu Leu Ala Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser 130 135 140 Glu Glu He His Pro Lys Asp He Gly His Wing Met Gly He Tyr He 145 150 155 160 Wing Gly Asn Thr Val Gly Gly Leu Thr Gly Arg Met He Pro Wing Gly 165 170 '175 10 Leu Leu Glu Val Thr His Trp Gln Asn Ala Leu Leu Gly Ser Ser He 180 185 190 Ala Ala Leu He Phe Gly Val He Met Val Val Leu Leu Pro Lys Gln 195 200 2 05 Arg Lys Phe Gln Pro Lys Asn He Asn Leu Arg His Glu He Ser Wing 210 215 220 Met Ala Wing His Trp Arg Asn Pro Arg Leu Wing Leu Leu Phe Gly Thr 225 230 235 240 15 Wing Phe Leu Gly Met Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly 245 250 255 Phe Arg Met He Asp Gln Phe Gly Leu Ser Glu Val Leu Val Gly Wing 260 265 270 Val Phe He Met Tyr Leu Wing Gly Thr Trp Ser Be Thr Gln Wing Gly 275 280 285 Wing Leu Arg Glu Lys He Gly Asn Gly Ser Thr Val He Phe Leu Ser 290 295 - 300 20 Leu Thr Met He Ala Wing Met Wing Wing Leu Met Gly He Asn Asn Leu Trp 305 310 315. 320 Val Thr Leu Val Ala Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu 325 330 -. 335 His Ser Ser Wing Ser Gly Trp He Gly He He Wing Thr Lys Asp Arg 340 345 * '350 Wing Glu Wing Being Ser Met Tyr Leu Phe Cys Tyr Tyr Val Gly Ser Ser 355 360 365 25 Val He Gly Trp Val Ser Gly Phe Wing Phe Thr His Leu Pro Trp Leu 370 375 380"Wing Phe He Gly Trp Leu He Leu Leu Leu Cys Gly Val Leu Wing He 385 390 395 400 Cys Val Thr Leu Wing Arg Leu Wing Arg Asn Wing Asn 405 410 < 210 > 207 < 211 > 1215 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1192) < 223 > FRXA01873 < 400 > 207 ccgtcgttgc ccatggtcac agcctacatg cacaaagtga atcaaaaaca gctatttcta 60 acattttact aatatttgct gttggcgcat gatgaactcc atg age caa gca ata 115 Met Ser Gln Ala He 1 5 gat age aag gtc gag gca cac gaa ggc cac gaa ggc cac gaa ggc atc 163 Asp Ser Lys Val Glu Ala His Glu Gly His Glu Gly His Glu Gly He 10 15 20 gag cga gga aca cgc aat tac aag cgc gct gtg ttt gcg atg ctg gcc 211 Glu Arg Gly Thr Arg Asn Tyr Lys Arg Ala Val Phe Ala Met Leu Ala 25 30 '' 35 gcc ggt ctt gct gct ttc aat ggt ctt tat tgc acg cag gt ttg ctt 259 Wing Gly Leu Wing Wing Phe Asn Gly Leu Tyr Cys Thr Gln Wing Leu Leu 40 45 50 ccc acc atg acg gag gag ttg gga att acg ccc act gag tcc gcg ctg 307 Pro Thr Met Thr Glu Glu Leu Gly He Thr Pro Thr Glu Be Ala Leu 55 60 65 acg gtg tcg gct acg act gga atg ttg gcg ctg tgt att gtt ccg gcg 355 Thr Val Ser Ala Thr Thr Gly Met Leu Ala Leu Cys He Val Pro Ala 70 75 80 85 tcg ata ctt tcg gag aaa ttt ggt cgc ggt cgg gtg ctg aca att tea 403 Ser He Leu Ser Glu Lys Phe Gly Arg Gly Arg Val Leu Th r He Be 90 95 100 ctc acg ttg gcc atc atc gtg gga tta att ttg ccg ctt gtc ccc aat 451 Leu Thr Leu Ala He He Val Val Leu He Leu Pro'Leu Val Pro Asn 105 110 115 * att act gct ctc atc ctg ctc aga ggt ctc cag ggt gcg ctg ctt gct 499 He Thr Ala Leu He Leu Leu Arg Gly Leu Gln Gly Ala Leu Leu Wing 120 125 130 ggc act cc gcg gtg gcg atg acc tgg ttg tct gag gaa att cac ccc 547 Gly Thr Pro Wing Val Wing Met Thr Trp Leu Ser Glu Glu He His Pro 135 140 145 'aag gat ggg cat gcg atg gga att tac atc gcg gga aat act gtc 595 Lys Asp He Gly His Wing Met Gly He Tyr He Wing Gly Asn Thr Val 150 155 160 165 ggc ggg ctc act gga cgt atg att ccg gcg gga cta ctt gaa gta act 643 Gly Gly Leu Thr Gly Arg Met He Pro Wing Gly Leu Leu Glu Val Thr 170 175 180 cat tgg caa aac gca ctg ctg gga agt tct atc gct gcg ctg atc ttc 691 His Trp Gln Asn Ala Leu Leu Gly Ser He Wing Wing Leu He Phe 185 190 195 ggc gta atc atg gtg gtg ttg ctt ccc aag cag cgg aaa ttc cag ccg 739 Gly Val He Met Val Val Leu Leu Pro Lys Gln Arg Lys Phe Gln Pro 200 205 .210 aag aat atc aat ctg cgc cat gag att tcg gcg atg gct gct cat tgg 787 Lys Asn He Asn Leu Arg His Glu He Be Wing Met Wing Wing His Trp 215 220 225 cgg aat ect cgt ttg gcg ttg ctt ttt ggt act gcg ttt ttg ggc atg 835 Arg Asn Pro Arg Leu Wing Leu Leu Phe Gly Thr Wing Phe Leu Gly Met 230 235 240 245 ggt act ttt gtg tcg ctg tac aac tat ttg ggt tic cgc atg att gat 883 Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly Phe Arg Met He Asp 250 255 '260 cag ttt ggg ctg agt gaa gtg ctg gtt ggt gcg gtg ttc atc tat 931 Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala Val Phe He Met Tyr 265 270 275 ctg gcc ggg acc tgg agt tcc acc cag gcg ggt gcg tgg agg gag aag 979 Leu Wing Gly Thr Trp Ser Ser Thr Gln Wing Gly Wing Leu Arg Glu Lys 280 285 290 atc ggc aat ggg tea acg gtt att ttc ttg agt ctg acg atg gcg 1027 He Gly Asn Gly Ser Thr Val He Phe Leu Ser Leu Thr Met He Wing 295 300 305 tcg atg gca ctg atg ggg att aat aat ttg tgg gtc acg ttg gtt gcc 1075 Ser Met Wing Leu Met Gly He Asn Asn Leu Trp Val Thr Leu Val Wing 310 315 320 325 ctg ttt gtg ttt acc gcg gca ttt tcc gca ctg cat. tcc agt gct tcg 1123 Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu His Ser Ser Wing Ser 330 335 340 gga tgg atc gga atc atc gca acg aag gat cgc gcg gaa gcc tcc age 1171 Gly Trp He Gly He He Ala Thr Lys Asp Arg Ala Glu Ala Being Ser 345 350 '• 355 atg tat ttg ttc tgt gaa tac taggatcctc ggtgattggt tgg 1215 Met Tyr Leu Phe Cys Glu Tyr 360 < 210 > 208 < 211 > 364 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 208 Met Ser Gln Ala He Asp Ser Lys Val Glu Ala His Glu Gly His Glu 1 5 10 15 Gly His Glu Gly He Glu Arg Gly Thr Arg Asn * Tyr Lys Arg Ala Val 20 25 30 Phe Ala Met Leu Ala Ala Gly Leu Ala Wing Phe Asn Gly Leu Tyr Cys 35 40 45 Thr Gln Wing Leu Leu Pro Thr Met Thr Glu Glu Leu Gly He Thr Pro 50 55 60 Thr Glu Be Ala Leu Thr Val Ser Wing Thr Thr Gly Met Leu Ala Leu 65 70 75 80 Cys He Val Pro Ala Be He Leu Ser Glu Lys Phe Gly Arg Gly Arg 85 90 95 Val Leu Thr He Ser Leu Thr Leu Wing He He Val Vally Leu He Leu 100 105 '110 Pro Leu Val Pro Asn He Thr Ala Leu He Leu Leu Arg Gly Leu Gln 115 120 125 Gly Ala Leu Leu Ala Gly Thr Pro Ala Val Ala Met Thr Trp Leu Ser 130 135 140 'Glu Glu He His Pro Lys Asp He Gly His Wing Met Gly He Tyr He 145 150 155. 160 Wing Gly Asn Thr Val Gly Gly Leu Thr Gly Arg Met He Pro Wing Gly 165 '170 175 Leu Leu Glu Val Thr His Trp Gln Asn Ala Leu Leu. Gly Ser Be He 180 185 190 Ala Ala Leu He Phe Gly Val He Met Val Val Leu Leu Pro Lys Gln 195 200 205 Arg Lys Phe Gln Pro Lys Asn He Asn Leu Arg His Glu He Ser Wing 210 215 220 Met Ala Ala His Trp Arg Asn Pro Arg Leu Wing Leu Leu Phe Gly Thr 225 230 235 240 Wing Phe Leu Gly Met Gly Thr Phe Val Ser Leu Tyr Asn Tyr Leu Gly 245 250 255 Phe Arg Met He Asp Gln Phe Gly Leu Ser Glu Val Leu Val Gly Ala 260 265 270 Val Phe He Met Tyr Leu Wing Gly Thr Trp Ser Ser Thr Gln Wing Gly 275 280 285 * .t Wing Leu Arg Glu Lys He Gly Asn Gly Ser Thr Val He Phe Leu Ser 290 295 300 Leu Thr Met He Wing Being Met Ala Leu Met Gly He Asn Asn Leu Trp 305 310 315 320 Val Thr Leu Val Ala Leu Phe Val Phe Thr Ala Ala Phe Phe Ala Leu 325 330 335 His Ser Ser Wing Ser Gly Trp He Gly He He Wing Thr Lys Asp Arg 340 345 350 Wing Glu Wing Being Ser Met Tyr Leu Phe Cys Glu Tyr 355 360 < 210 > 209 < 211 > 1572 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1549) < 223 > RXN00034 < 400 > 209 taaattttgt ggcactcccc acatttctat caatctatag aaagtatgac ttaaagtcga 60 ttttgcaagt ttctatagat tgatagaaaa gggagtttag atg tct tac aca tct 115 Met Ser Tyr Thr Ser 1 5 ttt aaa ggc gat gat aaa gcc ctc atc ggc ata gtt tta tea gtt ctc 163 Phe Lys Gly Asp Asp Lys Ala Leu He Gly He Val Leu Ser Val Leu 10 15. 20 here ttt tgg ttt gtt cag tea acc atta ggc cca gat atg 211 Thr Phe Trp Leu Phe Wing Gln Ser Thr Leu Asn He Gly Pro Asp Met 25 30 35 gca act gat tta ggg atg age gat ggc acc atg aac ata gct gtc gtg 259 Thr Wing Asp Leu Gly Met Ser Asp Gly Thr Met Asn He Wing Val Val 40 45 50 gcc gcc gcg tta ttc tgt gga aca ttt atc gtc gca gcc ggc ggc atc 307 Ala Ala Ala Leu Phe Cys Gly Thr Phe He Val Ala Wing Gly Gly He 55 60 65 gca gat gtc ttt ggc cga gta cga atc atg atg atg att ggc atc atc ctt 355 Wing Asp Val Phe Gly Arg Val Arg He Met Met He Gly Asn He Leu 70 75 80 85 aac atc ctg gga tct ctc ctc atc gcc acg gca acg act tct tcc gcc 403 Asn He Leu Gly Ser Leu Leu He Wing Thr Wing Thr Thr Ser Leu Wing 90 95 100 action atg gtg atc gcg gtc cga gt ctc ca gga gt gca gca gc gcg 451 Thr Gln Met Val He Thr Gly Arg Val Leu Gln Gly Leu Wing Wing Ala 105 110 115 gcc atc atg tct gca tcc cta gca tta gtt aag tat tat tgg tta ggt 499 Wing He Met Being Wing Being Leu Wing Leu Val Lys Thr Tyr Trp Leu Gly 120 125 130 act gac cgc caa cga gca gtc tcc att tgg tcc att ggt tea tgg ggt 547 Thr Asp Arg Gln Arg Ala Val Ser He Trp Ser He Gly Ser Trp Gly 135 140 145 ggc acc gga ttc tgc gcg ctt ttc gcg ggt ctt gtt gta gca age ccc 595 Gly Thr Gly Phe Cys Wing Leu Phe Wing Gly Leu Val Val Wing Pro Pro 150 155 160 165 ttt ggt tgg aga gga atc ttc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe Gly Trp Arg Gly He Phe Wing Leu Cys Wing He Val Ser He Val 170 175 180 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 Ala He Ala Leu Thr Arg His He Pro Glu Ser Arg Pro Ala Gln Ser 185 190 195 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 He Gly Met His Leu Asp Trp Ser Gly He He Val Leu Wing Leu Ser 200 205 210 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tea ctt ggc tgg 787 Val Leu Ser Leu Glu Leu Phe He Thr Gln Gly Glu Ser Leu Gly Trp 215 220 225 acg cag tgg atg ac tgg act ctc ctt gcc gtt tct ttg aca ttt ctt 835 Thr His Trp Met Thr Trp Thr Leu Leu Ala Val Ser Leu Thr Phe Leu 230 235 240. 245 gca gtt ttc gtc ttc att gaa cgc atc gcc age tgg cca gtt ctc gac 883 Wing Val Phe Val Phe He Glu Arg He Wing Ser Trp Pro Val Leu Asp 250 255 260 ttc aac ctt ttc aaa gac cac gcc ttc age ggt gcg acc atc acc aac 931 Phe Asn Leu Phe Lys Asp His Wing Phe Ser Gly Wing Thr He Thr Asn 265 270 275 ttc att atg age gct act ggc gga gtt gtt gtt gtc atg tgg gtt 979 Phe He Met Being Wing Thr Gly Gly Val Val Wing Val Val Met Trp Val 280 285 290 cag caa atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc age 1027 ? i £ É ^ MÉ Gln Gln Met Gly Trp Gly Val Ser Pro Thr He Ser Gly Leu Thr Ser 295 300 305 atc ggc ttc gca gcc ttt gtc atc ctt ttc att cga gtt gga gaa aag 1075 He Gly Phe Ala Ala Phe Val He Leu Phe He Arg Val Gly Glu Lys 310 315 320 325 gcc atg cag aaa gtt ggc gcc gga gca gtg atc atc ac gct ggc atc 1123 Wing Met Gln Lys Val Gly Wing Arg Wing Val He He Thr Wing Gly He 330 335 340 ttg gta gta acc gcg acc gcc ctc cta atg atc acc gcg gtc age gag 1171 Leu Val Wing Thr Wing Thr Wing Leu Leu Met He Thr Wing Val Ser Glu 345 350 • 355 tea acg tac atc gtc atc tcc ctc gcc gcc ttc tcc ctt tat ggc ctt 1219 Ser Thr Tyr He Val He Ser Leu Wing Gly Phe Ser Leu Tyr Gly Leu 360 365 370 ggc ctc gt ctc gt ctc gcc acc acca gtc acc gat gcg ctt gga aca 1267 Gly Leu Gly Leu Phe Ala Thr Pro Val Thr Asp Thr Ala Leu Gly Thr 375 380 385 ctt ccc aaa gac cgt acc ggc gct ggt gca ggt gta ttc aag atg tcc 1315 Leu Pro Lys Asp Arg Thr Gly Wing Gly Wing Gly Val Phe Lys Met Ser 390 395 400 405 tct tcc ctc ggc gca gca cca ggc at c gca atc tcc act tea gtg ttc 1363 Be Ser Leu Gly Ala Ala Leu Gly He Ala Be Ser Thr Ser Val Phe 410 415 '420 ctc gca ctt cgc gac ggc acc tcc atc aac tcc gac gtc gca ctc gcc 1411 Leu Ala Leu Arg Asp Gly Thr Ser He Asn Ser Asp Val Ala Leu Wing 425 430 435 gga aca gtt tea ctt ggc atc aac gtt gta ttc gca gca aca gcc acc 1459 Gly Thr Val Ser Leu Gly He Asn Val Val Phe Ala Ala Thr Ala Thr 440 445 450 atc acc gca gca gtc ctt att cca aaa gcc gct ggc aaa gtc tea caa 1507 He Thr Wing Wing Val Leu He Pro Lys Wing Wing Gly Lys Val Ser Gln 455 460 465 acc age atc ac cct ect gag cca gct atc gct gta aaa atc 1549 Thr Ser He Thr Leu Pro Glu Pro Wing He Wing Val Lys He 470 475 480 taaaacttca ccaggacaga taa 1572 < 210 > 210 < 211 > 483 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 210 Met Ser Tyr Thr Ser Phe Lys Gly Asp Asp Lys Wing Leu He Gly He 10 15 Val Leu Ser Val Leu Thr Phe Trp Leu Phe Wing Gln Ser Thr Leu Asn 20 25 • 30 He Gly Pro Asp Met Wing Thr Asp Leu Gly Met Being Asp Gly Thr Met 35 40 45 5 Asn He Wing Val Val Wing Wing Ala Leu Phe Cys Gly- Thr Phe He Val 50 55 6.0 Wing Wing Gly Gly Wing Wing Asp Val Phe Gly Arg Val Arg He Met Met 65 70 75 80 He Gly Asn He Leu Asn He Leu Gly Be Leu Leu He Wing Thr Wing 85 90 95 Thr Thr Ser Leu Wing Thr Gln Met Val He Thr Gly Arg Val Leu Gln 100 105 110 10 Gly Leu Wing Wing Wing Wing He Met Wing Being Leu Ala Leu Val Lys 115 120 125 Thr Tyr Trp Leu Gly Thr Asp Arg Gln Arg Ala Val Ser He Trp Ser 130 135 140 • He Gly Ser Trp Gly Gly Thr Gly Phe Cys Ala Leu Phe Ala Gly Leu 145 150 155 - 160 Val Val Ala Ser Pro Phe Gly Trp Arg Gly He Phe Ala Leu Cys Ala 165 170. 175 15 He Val Ser He Val Wing He Wing Leu Thr Arg His He Pro Glu Ser 180 185 190 Arg Pro Wing Gln Ser He Gly Met His Leu Asp Trp Ser Gly He He 195 200 205 Val Leu Ala Leu Ser Val Leu Ser Leu Glu Leu Phe He Thr Gln Gly 210 215 220 • Glu Ser Leu Gly Trp Thr His Trp Met Thr Trp Thr Leu Leu Ala Val 225 230 235 240 20 Ser Leu Thr Phe Leu Wing Val Phe Val Phe He Glu Arg He Wing Ser 245 250 255 Trp Pro Val Leu Asp Phe Asn Leu Phe Lys Asp His Wing Phe Ser Gly 260 265 270 Wing Thr He Thr Asn Phe He Met Ser Wing Thr Gly Gly Val Val Wing 275 280 285 Val Val Met Trp Val Gln Gln Met Gly Trp Gly Val 'Ser Pro Thr He 290 295 300 25 Ser Gly Leu Thr Ser He Gly Phe Ala Ala Phe Val He Leu Phe He 305 310 315 320 Arg Val Gly Glu Lys Ala Met Gln Lys Val Gly Ala Arg Ala Val He 325 330 '335 He Thr Wing Gly He Leu Val Wing Thr Wing Thr Wing Leu Leu Met He 340 345 350 5 Thr Wing Val Ser Glu Ser Thr Tyr He Val He Be Leu Wing Gly Phe 355 360 365 Be Leu Tyr Gly Leu Gly Leu Gly Leu Phe Wing Thr Pro Val Thr Asp 370 375 380 Thr Wing Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Wing Gly Wing Gly 385 390 395 400 Val Phe Lys Met Being Ser Leu Gly Ala Ala Leu Gly He Wing He 405 410 415 10 Ser Thr Ser Val Phe Leu Ala Leu Arg Asp Gly Thr Ser He Asn Ser 420 425 430 Asp Val Ala Leu Ala Gly Thr Val Ser Leu Gly He Asn Val Val Phe • 435 440 445 Ala Ala Thr Ala Thr He Thr Ala Ala Val Leu He Pro Lys Ala Ala 450 455 460 Gly Lys Val Ser Gln Thr Ser He Thr Leu Pro Glu Pro Ala He Wing 465 470 475 480 15 Val Lys He < 210 > 211 < 211 > 1045 < 212 > DNA < 213 > Corynebacterium glutamicum • < 220 > < 221 > CDS < 222 > (101) .. (1045) 20 < 223 > FRXA02273 < 400 > 211 taaattttgt ggcactcccc acatttctat caatctatag aaagtatgac ttaaagtcga 60 ttttgcaagt ttctatagat tgatagaaaa gggagtttag atg tct tac aca tct 115 Met Ser Tyr Thr Ser 1 5 ttt aaa ggc gat gat aaa gcc ctc atc ggc ata gtt tta tea gtt ctc 163 Phe Lys Gly Asp Asp Lys Ala Leu He Gly He Val Leu Ser Val Leu 10 15. 20 25 here ttt ttt ctt ttt gct cag tea acc atta ggc cca gat atg 211 Thr Phe Trp Leu Phe Wing Gln Ser Thr Leu Asn He Gly Pro Asp Met 25 30 35 gca act gat tta ggg atg age gat ggc acc atg aac ata gct gtc gtg 259 Wing Thr Asp Leu Gly Met Ser Asp Gly Thr Met Asn He Wing Val Val 40 45 50 gcc gcc gcg tta ttc tgt gga aca ttt atc gtc gca gcc ggc ggc atc 307 Wing Wing Wing Leu Phe Cys Gly Thr Phe He Val Ala Ala Gly Gly He 55 60 65 gca gat gtc ttt ggc cga gta cga atc atg atg atg att ggc atc atc ctt 355 Wing Asp Val Phe Gly Arg Val Arg He Met Met He Gly Asn He Leu 70 75 80 85 aac atc ctg gga tct ctc ctc atc gcc acg gca acg act tct tcc gcc 403 Asn He Leu Gly Ser Leu Leu He Wing Thr Wing Thr Thr Ser Leu Wing 90 95 100 10 action atg gtg atc acc ggc cga gtt ctc caa gga ctg gca gca gca gcg 451 Thr Gln Met Val He Thr Gly Arg Val Leu Gln Gly Leu Ala Ala Ala 105 110 '115 • gcc atc tct gca tcc cta gca tta gtt aag tat tat tgg tta ggt 499 Ala He Met Ser Ala Ser Leu Ala Leu Val Lys Thr Tyr Tr p Leu Gly 120 125 130 act gac cgc caa cga gca gtc tcc att tgg tcc att ggt tea tgg ggt 547 Thr Asp Arg Gln Arg Ala Val Ser He Trp Ser He Gly Ser Trp Gly 135 140 145 15 ggc acc gga ttc tgc gcg ctt ttc gcg ggt ctt gtt gta age ccc 595 Gly Thr Gly Phe Cys Wing Leu Phe Wing Gly Leu Val Val Wing Pro Pro 150 155 160 165 ttt ggt tgg aga gga atc tcc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe Gly Trp Arg Gly He Phe Wing Leu Cys Wing He Val Ser He Val 170 175 180 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 • Ala He Ala Leu Thr Arg His He Pro Glu Ser Arg Pro Wing Gln Ser 185 190 195 20 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 He Gly Met His Leu Asp Trp Ser Gly He He Val Leu Ala Leu Ser 200 205 210 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tea ctt ggc tgg 787 Val Leu Ser Leu Glu Leu Phe He Thr Gln Gly Glu Ser Leu Gly Trp 215 220 225 acg cag tgg atg acc tgg act ctc ctt gcc gtt tct ttg ttt ctt ctt ctc ctc ctc ctc ctc ctc 835 Thr TrT Leu Thr Thu Leu Thr Phe Leu 230 235 240 245 25 gca gtt ttc gtc ttc att atc gc gc tc ct gc ctc gac 883 Phe Val Phe He Glu Arg He Wing Ser Trp Pro Val Leu Asp 250 255. 260 ttc aac ctt ttc aaa gac cac gcc ttc age ggt gcg * acc atc ac aac 931 Phe Asn Leu Phe Lys Asp His Wing Phe Ser Gly Wing Thr He Thr Asn 265 270 275 ttc att atg age gct act ggc gga gta gtt gcc gtt gtc atg tgg gtt 979 Phe He Met Being Wing Thr Gly Val Val Wing Val Val Met Trp Val 280 285 290 cag cag atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc age 1027 Gln Gln Met Gly Trp Gly Val Ser Pro Thr He Ser Gly Leu Thr Ser 295 300 305 atc ggc ttc gca gcc ttt 1045 He Gly Phe Ala Ala Phe 310 315 < 210 > 212 < 211 > 315 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 212 Met Ser Tyr Thr Ser Phe Lys Gly Asp Asp Lys Wing Leu He Gly He 1 5 10 15 Val Leu Ser Val Leu Thr Phe Trp Leu Phe Wing Gln Ser Thr Leu Asn 20 25 30 He Gly Pro Asp Met Wing Thr Asp Leu Gly Met Ser Asp Gly Thr Met 35 40 45 Asn He Wing Val Val Wing Wing Ala Leu Phe Cys Gly Thr Phe He Val 50 55 60 Wing Wing Gly Gly Wing Wing Asp Val Phe Gly Arg Val Arg He Met Met 65 70 75 75 He Gly Asn He Leu Asn He Leu Gly Be Leu Leu He Wing Thr Wing 85 90 95 Thr Thr Ser Leu Wing Thr Gln Met Val He Thr Gly Arg Val Leu Gln 100 105 110 Gly Leu Wing Wing Wing Wing He Met Wing Being Wing Leu Wing Leu Val Lys 115 120 125 Thr Tyr Trp Leu Gly Thr Asp Arg Gln Arg Wing Val Ser He Trp Ser 130 135 140. He Gly Ser Trp Gly Gly Thr Gly Phe Cys Ala Leu Phe Wing Gly Leu 145 150 155 * 160 Val Val Wing Ser Pro Phe Gly Trp Arg Gly He Phe Wing Leu Cys Wing 165 170 175 He Val Val He Wing Wing Wing Wing Leu Thr Arg His He Pro Glu Ser 180 185. 190 Arg Pro Wing Gln Ser He Gly Met His Leu Asp Trp Ser Gly He He 195 200 205 Val Leu Ala Leu Ser Val Leu Ser Leu Glu Leu Phe He Thr Gln Gly 210 215 220 Glu Ser Leu Gly Trp Thr His Trp Met Thr Trp Thr Leu Leu Ala Val 225 230 235 240 Be Leu Thr Phe Leu Wing Val Phe Val Phe He Glu Arg He Wing Ser 245 250. 255 Trp Pro Val Leu Asp Phe Asn Leu Phe Lys Asp His Wing Phe Ser Gly 260 265 270 Wing Thr He Thr Asn Phe He Met Ser Wing Thr Gly Val Val Wing 275 280 285 Val Val Met Trp Val Gln Gln Met Gly Trp Gly Val Ser Pro Thr He 290 295 300 Ser Gly Leu Thr Ser He Gly Phe Ala Ala Phe 305 310 315 < 210 > 213 < 211 > 826 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (826) < 223 > RXN03075 < 400 > 213 tgtgeaaaat tgcagecagg ctgaaaaatt ectaaaggga ctccgtccga ataattggaa 60 ageccagaag aacagtcaac tectagatta aaggataatc gtg gcg aaa ttc ctg 115 Val Ala Lys Phe Leu .1 5 tat aag tta ggc tcc acg gcc tat caa aag aaa tgg ccg ttt ctt gcg 163 Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys Trp Pro Phe Leu Ala October 15 20 gtc tgg ctc gtg att ctc ata ggt atc acg acg ctg gcg ggg ctg tat 211 Val Trp Leu Val He Leu He Gly He Thr Thr Leu Ala Gly Leu Tyr 25 30 35 gcc aag cca acg ttc tcg agt tct age atc gat tct ctt ggt ect gtc 259 Ala Lys Thr Pro Ser Ser Ser Phe Ser Gly Leu Pro Asp He Ser Val 40 45 50 acc atg acg atg cag aag gag gag cgt ttc tcg gat ect g.gt gat gca 307 Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro Giy Ser Asp Asp Ala 55 60 65 aca tcg gct ccc act ggt tct gtc gtc att cag gca ecg gaa ggc aag 355 Thr Ser Ala Pro Thr Gly Ser Val Val He Gln Ala Pro Glu Gly Lys 70 75 80 85 acc ctc act gat ect gag gtt ggg gct gta gaa aac cag atg ctt gat 403 Thr Leu Thr Asp Pro Glu Val Gly Ala Glu Val Asn Gln Met Leu Asp 90 95 100 gag gtt cgg gcg act ggt gtg ctg aag gat gct gat aka gtt gtg gat 451 Glu Val Arg Ala Thr Gly Val Leu Lys Asp Ala Asp Ser Val Val Asp 105 110 115 ect gtg ttg gct gcg cag ggt gtg gct gct cag atg acc cca gcc ctg 499 Pro Val Leu Ala Ala Gln Gly Val Ala Wing Gln Met Thr Pro Ala Wing 120 125 '130 gag gct ggt gta ect gcg gag aag atc gcc gca gat att gag tcg 547 Glu Wing Gln Gly Val Pro Wing Glu Lys He Wing Wing Asp He Glu Ser 135 140 1'45 att agt cca ctg agt gca gat gag act acc ggc atc atc tcg atg act 595 He Ser Pro Leu Ser Ala Asp Glu Thr Thr Gly He He Ser Met Thr 150 155 160 165 ttt gat gca gat tct gcc atg gat ata aka gca gag gat cgt gag aag 643 Phe Asp Ala Asp Ser Ala Met Asp He Ser Ala Glu Asp Arg Glu Lys 170 175 '' 180 gtc acc aat att ctt gat gaa tac gat gac ggc gat ctg act gtt gtc 691 Val Thr Asn He Leu Asp Glu Tyr Asp Asp Gly Asp Leu Thr Val Val 185 190 195 tac aac ggc aac gtg ttt ggc gca gct gca acc age ttg gac atg acc 739 Tyr Asn Gly Asn Val Phe Gly Ala Wing Wing Thr Ser Leu Asp Met Thr 200 205. 210 tct gag ctc atc ggc ctg ctg gtg gct gcg gtt gtt ctt atc gtg acc 787 Ser Glu Leu He Gly Leu Leu Val Ala Wing Val Val Leu He Val Thr 215 220 225 ttc ggt tcg ttc atc gct gcc ggt atg ccg ctg atc tct 826 Phe Gly Ser Phe He Wing Wing Gly Met Pro Leu He Ser 230 235 240 ' < 210 > 214 < 211 > 242 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 214 £ f ^^ Val Ala Lys Phe Leu Tyr Lys Leu Gly Ser Thr Wing Tyr Gln Lys Lys 1 5 10 15 Trp Pro Phe Leu Wing Val Trp Leu Val He Leu He Gly He Thr Thr 20 25 30 Leu Wing Gly Leu Tyr Wing Lys Pro Thr Being Ser Phe Ser He Pro 35 40 45 Gly Leu Asp Ser Val Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro 50 55 60 Gly Ser Asp Asp Wing Thr Ser Wing Pro Thr Gly Ser Val Val He Gln 65 70 75 80 Wing Pro Glu Gly Lys Thr Leu Thr Asp Pro Glu Val Gly Wing Glu Val 85 90 • 95 Asn Gln Met Leu Asp Glu Val Arg Wing Thr Gly Val Leu Lys Asp Wing 100 105 110 Asp Ser Val Val Asp Pro Val Leu Ala Wing Gln Gly Val Wing Ala Gln 115 120, '125 Met Thr Pro Ala Leu Glu Ala Gln Gly Val Pro Wing Glu Lys He Wing 130 135 140 Wing Asp He Glu Ser Be Pro Pro Leu Ser Wing Asp Glu Thr Thr Gly 145 150 155 160 He Has To Be Met Thr Phe Asp Wing Asp To Be Wing Met Asp He To Be Wing 165 170 '175 Glu Asp Arg Glu Lys Val Thr Asn He Leu Asp Glu Tyr Asp Asp Gly 180 185 190 Asp Leu Thr Val Val Tyr Asn Gly Asn Val Phe Gly Ala Wing Wing Thr 195 200 205 Ser Leu Asp Met Thr Ser Glu Leu He Gly Leu Leu Val Ala Ala Val 210 215 220 Val Leu He Val Thr Phe Gly Ser Phe He Ala Ala Gly Met Pro Leu 225 230 235 240 I have to be < 210 > 215 < 211 > 826 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (826) < 223 > FRXA02907 < 400 > 215 tgtgeaaaat tgcattcagg ctgaaaaatt ectaaaggga ctccgtccga ataattggaa 60 ageccagaag aacagtcaac tectagatta aaggataatc gtg gcg aaa ttc ctg 115 Val Ala Lys Phe Leu 1 5 • tat aag tta ggc tcc acg gcc tat caa aag aaa tgg ccg ttt ctt gcg 163 Tyr Lys Leu Gly Ser Thr Ala Tyr Gln Lys Lys Trp Pro Phe Leu Wing 10 15 20 gtc tgg ctc gtg att ctc ata ggt atc acg acg ctg .gcg ggg ctg tat 211 Val Trp Leu Val He Leu He Gly He Thr Thr Leu Wing Gly Leu Tyr 25 30 35 gcc aag cca acg tcg agt age ttc tct atc ect ggt ctt gat tct gtc 259 Wing Lys Pro Thr Ser Ser Ser Phe Be Pro Pro Gly Leu Asp Ser Val 40 45 50 10 acg acc atg gag aag atg cag gag cgt ttc ect ggt tcg gat gat gca 307 Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro Gly Ser Asp Asp Ala 55 60 65 • aca tcg gct ccc act ggtct gtc gtc att cag gca ccg gaa ggc aag 355 Thr Ser Ala Pro Thr Gly Ser Val Val He Gln Wing Pro Glu Gly Lys 70 75 80 85 act cctc gat gat gct ggg ggg gct gaa gta aac cag atg ctt gat 403 Thr Leu Thr Asp Pro Glu Val Gl and Wing Glu Val Asn Gln Met Leu Asp 90 95 100 15 gag gtt cgg gcg act ggt gtg ctg aag gat gct gat tct gtt gtg gat 451 Glu Val Arg Wing Thr Gly Val Leu Lys Asp Wing Asp Ser Val Val Asp 105 110 115 ect gtg gtg tg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg gg ggg ggg ggg ggg ggg ggg ggg ggg ggg ggg ggg gag tcg 547 Glu Wing Gln Gly Val Pro Wing Glu Lys He Wing Wing Asp He Glu Ser 135 140 145 20 att agt cca gt gat gca gat gag act acc ggc atc tcg atg act 595 He Ser Pro Leu Ser Wing Asp Glu Thr Thr Gly He He Be Met Thr 150 155 160 165 ttt gat gca gat tct gcc atg gat ata tcc gca gag gat cgt gag aag 643 phe asp wing asp be wing ala asp he be wing glu Asp Arg Glu Lys 170 175 • '180 gtc acc aat att ctt gat gaa tac gat gac ggc gat ctg act gtt gtc 691 Val Thr Asn He Leu Asp Glu Tyr Asp Asp Gly Asp Leu Thr Val Val 185 190 195 25 ^ iaafe = Ma? l &«« J? a tac aac ggc aac gtg ttt ggc gca gct gca acc agd ttg gac atg acc 739 Tyr Asn Gly Asn Val Phe Gly Wing Wing Thr Ser Leu Asp Met Thr 200 205 210 tct gag ctc atc ggc ctg ctg gtg gct gcg gtc gtt ctt atc gtg acc 787 Ser Glu Leu He Gly Leu Leu Val Ala Wing Val Val Leu He Val Thr 215 220 225 ttc ggt tcg ttc atc gct gcc ggt atg ccg ctg atc tct 826 Phe Gly Ser Phe He Wing Wing Gly Met Pro Leu He Ser 230 235 240 < 210 > 216 < 211 > 242 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 216 Val Ala Lys Phe Leu Tyr Lys Leu Gly Ser Thr Wing Tyr Gln Lys Lys 1 5 10 15 Trp Pro Phe Leu Wing Val Trp Leu Val He Leu He Gly He Thr Thr 20 25 30 Leu Wing Gly Leu Tyr Wing Lys Pro Thr Ser Be Ser Phe Ser He Pro 35 40 45 Gly Leu Asp Ser Val Thr Thr Met Glu Lys Met Gln Glu Arg Phe Pro 50 55 60 Gly Ser Asp Asp Ala Thr Ser Ala Pro Thr Gly Ser Val Val He Gln 65 70 75 80 Wing Pro Glu Gly Lys Thr Leu Thr Asp Pro Glu Val Gly Wing Glu Val 85 90 95 Asn Gln Met Leu Asp Glu Val Arg Wing Thr Gly Val Leu Lys Asp Wing 100 105 110 Asp Ser Val Val Asp Pro Val Leu Ala Ala Gln Gly Val Ala Ala Gln 115 120 '125 Met Thr Pro Ala Leu Glu Ala Gln Gly Val Pro Ala Glu Lys He Ala 130 135 140 Ala Asp He Glu Ser He Pro Pro Leu Ser Ala Asp' Glu Thr Thr Gly 145 150 155 160 He Has Being Met Thr Phe Asp Wing Asp Being Wing Met Asp He Being Wing 165 170 175 Glu Asp Arg Glu Lys Val Thr Asn He Leu Asp Glu Tyr Asp Asp Gly 180 185 J 190 Asp Leu Thr Val Val Tyr Asn Gly Asn Val Phe Gly Wing Wing Thr Wing 195 200 205 Ser Leu Asp Met Thr Ser Glu Leu He Gly Leu Leu Val Wing Wing Val 210 215 22'0 Val Leu He Val Thr Phe Gly Ser Phe He Wing Wing Gly Met Pro Leu 225 230 235 240 He Be < 210 > 217 < 211 > 2313 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (2290) < 223 > RXA00479 <; 400 > 217 tagatcccaa ggctcaaaat ttattactta aacaagttga gcaactagcc agccgcaaat 60 cttagaacta acctttacgc ctttaacgga agtgaatttg atg tct act age atc 115 Met ser Thr Ser He 1 5 aca aca gag aac aaag aa aa act act ect cctcct tctg atg aga atc 163 Thr Thr Glu Asn Lys Lys Lys Ser Gly Pro Pro Arg Leu Met Arg He 10 15 20 ttt ctg ccc gcc ttg cta att tta gtt tgg ctt gta gta gga gga gtc 211 Phe Leu Pro Wing Leu Leu He Leu Val Trp Leu Val Gly Wing Gly Val 25 30 35 ggc ggt ect tat ttt ggc aag gtt agt gag gtc tcc tcc aac age cag 259 Gly Gly Pro Tyr Phe Gly Lys Val Ser Glu Val Ser Ser Asn Ser Gln 40 45 »50 acc aca tat ctg cca gaa tct gcc gat gcc act caa -gta cag gaa cag 307 Thr Thr Tyr Leu Pro Glu Ser Wing Asp Wing Thr Gln Val Gln Glu Gln 55 60 65 ttg gga gat ttt act gat tct gaa tcc atc cca gcc att gtc gta atg 355 Leu Gly Asp Phe Thr Asp Ser Glu Ser He Pro Wing He Val Val Met 70 75 80 85 gtc age gat gaa ccc tta aca cag caa gac atc aca caa ctc aat gaa 403 Val Ser Asp Glu Pro Leu Thr Gln Gln Asp He Thr Gln Le u Asn Glu 90. 95 100 gtt gtt gct ggg gtt ctt tea gaa tta gac ata gtt tcc gat gaa gtc tcc 451 Val Val Wing Gly Leu Ser Glu Leu Asp He Val Ser Asp Glu Val Ser 105 110 115 ect gct att cca tcc gac gac ggc aga gct gtc caa gtg ttt gtc ccc 499 _i »4j Pro Wing Pro Pro Glu Asp Gly Arg Wing Val Gln Val Phe Val Pro 120 125 130 ctc aat cca tea gcg gag ctg acg gaa age gtc gag aag ctc tct gag 547 Leu Asn Pro Ser Wing Glu Leu Thr Glu Ser Val Glu Lys Leu Ser Glu 135 140 145 acc ttg acc acg cg gc gc gc gc age gtg acc gga gtg acc gga 595 5 Thr Leu Thr Gln Gln Thr Pro Asp Tyr Val Ser Thr Tyr Val Thr Gly • 150 155 160 165 ccg gct ggg ttt acc gct gat ctc age gca gct .ttc gcg ggt att gat 643 Pro Wing Gly Phe Thr Wing Asp Leu Wing Wing Phe Wing Gly He Asp 170 175 • 180 ggg cta ctc cta gca gtc gcc ttg gct gcc gtc ctt gtc att ctt gtc 691 Gly Leu Leu Leu Wing Val Wing Leu Wing Wing Val Leu Val He Leu Val 185 190 195 atc gtc tat cgc tcc ttc att ctg ccc atc gcc gtg ctt gcc acc agt 739 10 He Val Tyr Arg Ser Phe He Leu Pro He Ala Val Leu Ala Thr Ser 200 205. 210 ttg ttt gcg ctg act gta gct cta ttg gtg gtg tgg * tgg cta gct aag 787 • Leu Phe Ala Leu Thr Val Ala Leu Leu Val Val Trp Trp Leu Ala Lys 215 220 225 tgg gac atc ctg ctg ctt tcg ggt cag act ca ggc -ate ctc ttc att 835 Trp Asp He Leu Leu Leu Ser Gly Gln Thr Gln Gly He Leu Phe He 230 235 240 '245 ctg gtc att ggc gcc gcc acc gac tac tea ttg cta tac gtt gct cgt 883 15 Leu Val He Gly Ala Wing Thr Asp Tyr Ser Leu Leu Tyr Val Wing Arg 250 255 260 ttc cgt gaa gag tta cgc gtt caa caa gat aaa ggg ata gcc ggg aca 931 Phe Arg Glu Glu Leu Arg Val Gln Gln Asp Lys Gly He Wing Thr Gly 265 270 275 aaa gcc atc cgg gca tcg gtg gaa ccc att ctt gcc tcg ggc age act 979 Lys Ala He Arg Ala Ser Val Glu Pro He Leu Ala Ser Gly Ser Thr • 280 285 290 gtt att gcg ggc ctc ctt tgt ttg cta ttt agt gat ttg aaa tct aac 1027 20 Val He Wing Gly Leu Leu Cys Leu Leu Phe Ser Asp Leu Lys Ser Asn 295 300 305 tcc acg cta ggt cca gta gct tcg gtg ggc att att ttt gca atg ctt 1075 Ser Thr Leu Gly Val Pro Val Wing Val Gly He He Phe Wing Met Leu 310 315 320 325 tct gct ctt act ctg cta cca gcc ctg ctg ttt gta ttc ggt cgg gtg 1123 Be Wing Leu Thr Leu Leu Pro Wing Leu Leu Phe Val Phe Gly Arg Val 330 335 340 gcc ttt tgg tcc cgg aga cga aaa tac gaa ect gaa aaa gcc cgt gcg 1171 25 Wing Phe Trp Pro Lys Arg Pro Lys Tyr Glu Pro Glu Lys Wing Arg Wing 345 350 355 aaa aac gac atc ccc gcc age ggg atc tgg tea aaa gtg gct gat tta 1219 Lys Asn Asp He Pro Wing Ser Gly He Trp Ser Lys Val Wing Asp Leu 360 365 370 gtg gag cag cat ect cgt gca atc tgg gta tct aca ctt att gtg ctt 1267 Val Glu Gln His Pro Arg Ala He Trp Val Ser Thr Leu He Val Leu 375 380 385 ctc gt ggt gcg gct tc gtt ccc acacta aaa gcg gac ggt gtg tcc 1315 Leu Leu Gly Wing Wing Phe Val Pro Thr Leu Lys Wing Asp Gly Val Ser 390 395 400 405 caa tcc gac cta gtt ctg ggt tcc tct gaa gca cgt gat ggc cag cag 1363 Gln Ser Asp Leu Val Leu Gly Ser Ser Glu Wing Arg Asp Gly Gln Gln 410 415 420 gct tta ggc gaa cac ttc ccc ggt gga tcc ggc agt ect gct tat att 1411 Wing Leu Gly Glu His Phe Pro Gly Gly Ser Gly Ser Pro Wing Tyr He 425 430 435 atc g tt gat gaa aca cag gca gca cag gct gct gac -.gta gtc ctt aac 1459 He Val Asp Glu Thr Gln Ala Ala Gln Ala Ala Asp Val Val Leu Asn 440 445 450 aac gac aat ttc gag act gta act gta act agt gct gac tcc ccc tct 1507 Asn Asp Asn Phe Glu Thr Val Thr Val Thr Ser Ala 'Asp Ser Pro Ser 455 460 465 ggc tea gcc cca atc acc gct gac ggt att gtg ccg tta ggt tct ggt 1555 Gly Ser Wing Pro He Thr Wing Asp Gly He Val Pro Leu Gly Ser Gly 470 475 480 485 aca gct cca ggc ccg gta gta gta gaa ggg caa gtc ctt tta caa gca 1603 Thr Ala Pro Gly Pro Val Val Val Glu Gly Gln Val Leu Leu Gln Ala 490 495 500 aca ctt gtc gaa gca cca gat tcc gaa gaa gct caa aaa gct att cgc 1651 Thr Leu Val Glu Ala Pro Asp Ser Glu Glu Ala Gln Lys Ala He Arg 505 510 515 agt atc cgc caa act ttt gca gat gaa aat ata gcg gta gta gta gtac 1699 Be He Arg Gln Thr Phe Wing Asp Glu Asn He Be Wing Val Val Gly 520 525 530 ggt gtc act gca act tcc gta gac act aac gat gcc tcc atc cat gac 1747 Gly Val Thr Wing Thr Ser Val Asp Thr Asn Asp Wing Ser He His A sp 535 540 545 cgc aac ctg atc atc cca att gta ttg ctg gtc att ttg gtt att ctc 1795 Arg Asn Leu He He Pro He Val Leu Leu Val He Leu Val He Leu 550 555 560 565 atg ctg ttg ctg cgg tct att gtc gca cca ctc ctg cta gta gtc acc 1843 Met Leu Leu Arg Ser He Val Wing Pro Leu Leu Leu Val Val Thr 570 575 580 acc gtg gtg tct ttt gct act gct gta gct gct gct gct gct gct tct cct 1891 Thr Val Val Ser Phe Ala Thr Ala Leu Gly Val Ala Ala Leu Leu Phe 585 590 595 aat cac gtt ttc agt ttc cca gga gca gac ccc gca gta ect ctc tac 1939 Asn His Val Phe Ser Phe Pro Gly Wing Asp Pro Wing Val Pro Leu Tyr 600 605 610 gga ttt gta ttt tta gta gcc ttg ggc atc gac tac aac att ttc tta 1987 Gly Phe Val Phe Leu Val Ala Leu Gly He Asp Tyr Asn He Phe Leu 615 620 625 gtc acc cga atc cgt gaa gaa acc aaa acc acac gcc aca aga ctt gga 2035 Val Thr Arg He Arg Glu Glu Thr Lys Thr His Gly Thr Arg Leu Gly 630 635 640 645 att ctt cga ggc ctg gta acc ggc gta gta gta att acc tea gct gga 2083 He Leu Arg Gly Leu Thr Val Thr Gly Gly Val He Thr Ser Wing Gly 650 655 • '660 gta gtt gt ctc gcc gt acca ttc gca gca gtc tat gtc gtc atc cca att cta 2131 V al Val Leu Ala Ala Thr Phe Ala Ala Leu Tyr Val He Pro He Leu 665 670 675 ttc ctg gca caa att gcc ttc att gtc gct ttt gga gtt ctt att gat 2179 Phe Leu Ala Gln He Ala Phe He Val Ala Phe Gly Val Leu He Asp 680 685 690 acc ctc ctc gtt cgc gcc ttc ttg gtg ect gct ttg ttc tac gac atc 2227 Thr Leu Leu Val Arg Ala Phe Leu Val Pro Ala Leu Phe Tyr Asp He 695 700 705 gga ccg aaa atc tgg tgg ccg tea aaa ttg tcc aat cag aaa tac cag 2275 Gly Pro Lys He Trp Trp Pro Ser Lys Leu Ser Asn Gln Lys Tyr Gln 710 715 720 725 aag cag ect cag cta tgacacacca aaattcgcct ctc 2313 Lys Gln Pro Gln Leu 730 < 210 > 218 < 211 > 730 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 218 Met Ser Thr Ser He Thr Thr Glu Asn Lys Lys Ser Gly Pro Pro 1 5 10 15 Arg Leu Met Arg He Phe Leu Pro Wing Leu Leu He Leu Val Trp Leu 20 25 30 Val Gly Wing Gly Val Gly Gly Pro Tyr Phe Gly Lys Val Ser Glu Val 35 40 45 - - - - "" - Being Ser Asn Being Gln Thr Thr Tyr Leu Pro Glu Being Wing Asp Wing Thr 50 55 60 Gln Val Gln Glu Gln Leu Gly Asp Phe Thr Asp Ser Glu Ser He Pro 65 70 75 80 Ala He Val Val Met Val Ser Asp Glu Pro Leu Thr Gln Gln Asp He 85 90 95 Thr Gln Leu Asn Glu Val Val Wing Gly Leu Ser Glu Leu Asp He Val 100 105 110 Ser Asp Glu Val Ser Pro Wing Pro Pro Glu Asp Gly Arg Wing Val 115 120 125 Gln Val Phe Val Pro Leu Asn Pro Ser Wing Glu Leu Thr Glu Ser Val 130 135 140 Glu Lys Leu Ser Glu Thr Leu Thr Gln Gln Thr Pro Asp Tyr Val Ser 145 150 155 160 Thr Tyr Val Thr Gly Pro Wing Gly Phe Thr Wing Asp Leu Ser Wing Wing 165 170 175 Phe Wing Gly He Asp Gly Leu Leu Leu Wing Val Wing Leu Wing Wing Val 180 185 190 Leu Val He Leu Val He Val Tyr Arg Ser Phe He Leu Pro He Wing 195 200 205 Val Leu Wing Thr Ser Leu Phe Wing Leu Thr Val Wing Leu Leu Val Val 210 215 220 Trp Trp Leu Wing Lys Trp Asp He Leu Leu Leu Ser Gly Gln Thr Gln 225 230 235 240 Gly He Leu Phe He Leu Val He Gly Ala Wing Thr Asp Tyr Ser Leu 245 250 255 Leu Tyr Val Wing Arg Phe Arg Glu Glu Leu Arg Val Gln Gln Asp Lys 260 265 270 Gly He Wing Thr Gly Lys Wing He Arg Wing Being Val Glu Pro He Leu 275 280 '285 Wing Ser Gly Ser Thr Val He Wing Gly Leu Leu Cys Leu Leu Phe Ser 290 295 300 •. Asp Leu Lys Ser Asn Be Thr Leu Gly Pro Val Wing Ser Val Gly He 305 310 315 320 He Phe Wing Met Leu Ser Wing Leu Thr Leu Leu Pro Wing Leu Leu Phe 325 330 335 Val Phe Gly Arg Val Wing Phe Trp Pro Lys Arg Pro Lys Tyr Glu Pro 340 345 350 Glu Lys Ala Arg Ala Lys Asn Asp He Pro Wing Ser Gly He Trp Ser 355 360 365 Lys Val Wing Asp Leu Val Glu Gln His Pro Arg Ala He Trp Val Ser 370 375 380 Thr Leu He Val Leu Leu Leu Gly Ala Wing Phe Val Pro Thr Leu Lys 385 390 395 400 Wing Asp Gly Val Ser Gln Ser Asp Leu Val Leu Gly Ser Ser Glu Wing 405 410 415 Arg Asp Gly Gln Gln Ala Leu Gly Glu His Phe Pro Gly Gly Ser Gly 420 425 430 Ser Pro Wing Tyr He Hep Val Asp Glu Thr Gln Wing Wing Gln Wing Wing 435 440 445 Asp Val Val Leu Asn Asn Asp Asn Phe Glu Thr Val Thr Val Thr Ser 450 455 460 10 Wing Asp Ser Pro Ser Gly Ser Wing Pro He Thr Wing Asp Gly He Val 465 470 475 480 • Pro Leu Gly Ser Gly Thr Wing Pro Gly Pro Val Val Val Glu Gly Gln 485 490 495 Val Leu Leu Gln Ala Thr Leu Val Glu Ala Pro Asp Ser Glu Glu Wing 500 505 '510 Gln Lys Wing Arg Be He Arg Gln Thr Phe Wing Asp Glu Asn He 515 520 525 15 Be Wing Val Val Gly Gly Val Thr Wing Thr Ser Val Asp Th r Asn Asp 530 535 540 Wing Be He His Asp Arg Asn Leu He He Pro Pro He Val Leu Leu Val 545 550 555 560 He Leu Val He Leu Met Leu Leu Leu Arg Ser He Val Wing Pro Leu • 565 570 575 Leu Leu Val Val Thr Thr Val Val Ser Phe Wing Thr Wing Leu Gly Val 580 585 '590 20 Wing Wing Leu Leu Phe Asn His Val Phe Ser Phe Pro Gly Wing Asp Pro 595 600 605 Wing Val Pro Leu Tyr Gly Phe Val Phe Leu Val Wing Ala Leu Gly He Asp 610 615 620 Tyr Asn He Phe Leu Val Thr Arg He Arg Glu Glu Thr Lys Thr His 625 630 635 640 Gly Thr Arg Leu Gly He Leu Arg Gly Leu Thr Val Thr Gly Gly Val 645 650 655 25 i *. «IMM.

He Thr Be Ala Gly Val Val Leu Ala Ala Thr Phe Ala Ala Leu Tyr 660 665 * 670 Val He Pro He Leu Phe Leu Ala Gln He Ala Phe He Val Ala Phe 675 680 685 Gly Val Leu He Asp Thr Leu Leu Val Arg Ala Phe Leu Val Pro Wing 690 695 700 Leu Phe Tyr Asp He Gly Pro Lys He Trp Trp Pro Ser Lys Leu Ser 705 710 715 720 Asn Gln Lys Tyr Gln Lys Gln Pro Gln Leu 725 730 < 210 > 219 < 211 > 983 < 212 > DNA < 213 > Corynebacterium glutamicum 10 < 220 > < 221 > CDS < 222 > (1) .. (960) < 223 > RXN03124 < 400 > 219 atg act ect acc ctg gcg tcg atg att ggt ctg gct gtc ggt atc gac Met Thr Pro Thr Leu Ala Ser Met He Gly Leu Wing Val Gly He Asp 1 5 10 15 tac gcg cta ttt atc gtg tcc cgt ttc cgc aat gag ttg att tct cag 96 15 Tyr Ala Leu Phe He Val Ser Arg Phe Arg Asn Glu Leu He Ser Gln 20 25 30 act ggc gct aat gat ctg gag cca aag gaa ttg gct gag cgt ctg cgc 144 Thr Gly Wing Asn Asp Leu Glu Pro Lys Glu Leu Wing Glu Arg Leu Arg 35 40 45 tcg gtg gcg cat gcg atg gg atg gt gg ggc 192 Thr Met Pro Leu Ala Wing Arg Ala His Wing Met Gly Met Wing Val Gly 50 55 60 'act gcg ggt tct gcg gtt gta ttc gcg ggt acc acg gtg ctg atc gct 240 20 Thr Ala Gly Be Wing Val Val Phe Wing Gly Thr Thr Val Leu He Wing 65 70 75 80 ctg gtt gct ctg tcg atc att aat att cca ttt cta acc gtg atg gcc 288 Leu Val Ala Leu Ser He He Asn He Pro Phe Leu Thr Val Met Wing 85 90 95 att gct gcc gc atc acc gtt gcc atc gca gtt ctg gtt gct ctg tcc 336 He Ala Ala Ala He Thr Val Ala Ala Ala Val Leu Val Ala Leu Ser 100 105 110 ttc ctc cca gct ctg ctt ggc ctg ctt ggc act cgc atc ttc gca gca 384 25 Phe Leu Pro Ala Leu Leu Gly Leu Leu Gly Thr Arg He Phe Ala Ala ggg 115 120 125 cgc gtg ect gga ect aag gtt ccg gat ect gag gac gag aag cca acg 432 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr 130 135 140 atg ggt ctg aag tgg gtc cgc ctt gtg cgc aag atg ccg gtg gct tac 480 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Wing Tyr 5 145 150 155 160 ctg ctg gtt ggc gtc gtt ttg ctt ggt gca atc gca att ect gcg acc 528 Leu Leu Val Gly Val Val Leu Leu Gly Wing Wing He Pro Wing Thr 165 170 175 aat atg cgc ctg gcc atg ccg act gat ggc acc tcc acg ctg ggc acc 576 Asn Met Arg Leu Wing Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr 180 185 190 gcg ccg cgc acg ggg tat gac atg acg gca gat gcg tcc gcc gcc gcc 624 Pro Wing Arg Thr Gly Tyr Asp Met Thr Wing Asp Wing Phe Gly Pro Gly 10 195 200 205 cgc aac gcg ccc atg att gcg ctt atc gac gca acc gac gtc ect gag 672 Arg Asn Wing Pro Met He Wing Leu He Asp Wing Thr Asp Val Pro Glu • 210 215 220 gaa gag cgc cca ttg gtg ttt gga cag gcg gtg gag caa ttc ttg aac 720 Glu Glu Arg Pro Leu Val Phe G Gln Ala Val Glu Gln Phe Leu Asn 225 230 '235 240 act gat ggt gtg aag aat gct cag atc act cag ac ac gag aat ttc 768 Thr Asp Gly Val Lys Asn Ala Gln He Thr Gln Thr Thr Glu Asn Phe 15 245 250 255 gat acc gcg cag atc ctg tta ccc cag aat ttg atg cga tcg atg age 816 Asp Thr Ala Gln He Leu Leu Pro Gln Asn Leu Met Arg Ser Met Ser 260 265 270 gca ect ctg aga ctc tcg caa ctc ttc gtg cag atg ctg aga ect tcg 864 Wing Pro Leu Arg Leu Ser Gln Leu Phe Val Gln Met Leu Arg Pro Ser 275 280"285 ctg atg aca ccg gcg cga cgt atg gca tta ctg gcg tea ccc caa ttt 912 Leu Met Thr Pro Ala Arg Arg Met Ala Leu Leu Wing Pro Pro Gln Phe 20 290 295 300 acg atg ctc tcg gcg tcg gcg acc tgg ttc ctt acct tcc 960 Thr Met Thr Ser Leu Leu Wing Ser Wing Thr Ser Trp Phe Leu Thr Phe 305 310 315 320 tgatcgtttt ggttctagcg ttc 983 < 210 > 220 < 211 > 320 < 212 > PRT 25 < 213 > Corynebacterium glutamicum • | iiMl ?? IIIBÍ? MÍft-rffrt < 400 > 220 Met Thr Pro Thr Leu Wing Ser Met He Gly Leu Wing Val Gly He Asp 1 5 10 15 Tyr Ala Leu Phe He Val Ser Arg Phe Arg Asn Glu Leu He Ser Gln 20 25 30 Thr Gly Wing Asn Asp Leu Glu Pro Lys Glu Leu Ala Glu Arg Leu Arg 35 40 45 Thr Met Pro Leu Ala Ala Arg Ala His Ala Met Gly Met Ala Val Gly 50 55 60 Thr Ala Gly Ser Ala Val Val Phe Ala Gly Thr Thr Val Leu He Ala 65 70 75 80 Leu Val Ala Leu Ser He He Asn He Pro Phe Leu Thr Val Met Wing 85 90 95 He Wing Wing Wing He Thr Val Wing He Wing Val Leu Val Wing Leu Ser 100 105 110 Phe Leu Pro Wing Leu Leu Gly Leu Leu Gly Thr Arg He Phe Wing Wing 115 120 125 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr 130 135 140 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Wing Tyr 145 150 155 160 Leu Leu Val Gly Val Val Leu Leu Gly Wing He Wing He Pro Wing Thr 165 170 175 Asn Met Arg Leu Wing Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr 180 185 ', 190 Wing Pro Arg Thr Gly Tyr Asp Met Thr Wing Asp Wing Phe Gly Pro Gly 195 200 205 Arg Asn Wing Pro Met He Wing Leu He Asp Wing Thr Asp Val Pro Glu 210 215 220 Glu Glu Arg Pro Leu Val Phe Gly Gln Wing Val Glü Gln Phe Leu Asn 225 230 235 240 Thr Asp Gly Val Lys Asn Wing Gln He Thr Gln Thr Thr Glu Asn Phe 245 250 255 Asp Thr Wing Gln He Leu Leu Pro Gln Asn Leu Met Arg Ser Met Ser 260 265 270 Wing Pro Leu Arg Leu Ser Gln Leu Phe Val Gln Met Leu Arg Pro Ser 275 280 285 Leu Met Thr Pro Ala Arg Arg Met Ala Leu Leu Ala. Ser Pro Gln Phe - & 4t j, 290 .95 300 Thr Met Thr Ser Leu Leu Wing Ser Wing Thr Ser Trp Phe Leu Thr Phe 305 310 315 320 < 210 > 221 < 211 > 762 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (762) < 223 > FRXA01180 < 400 > 221 atg act ect acc ctg gcg tcg atg att ggt ctg gct gtc ggt atc gac 48 Met Thr Pro Thr Leu Wing Ser Met He Gly Leu Wing Val Gly He Asp 1 5 10 15 tac gcg cta ttt atc gtg tcc cgt ttc cgc aat gg 'ttg att tct cag 96 Tyr Ala Leu Phe He Val Ser Arg Phe Arg Asn Glu Leu He Ser Gln 20 25 30 act ggc gct aat gat ctg gag cca aag gaa ttg gct gag cgt cgt ctg cgc 144 Thr Gly Wing Asn Asp Leu Glu Pro Lys Glu Leu Wing Glu Arg Leu Arg 35 40 45 atc action cg tcg gcg gcg gcg cat gcg atg gg atg gct ggc ggc 192 Thr Met Pro Leu Ala Wing Arg Ala His Wing Met Gly Met Wing Val Gly 50 55 60 act gcg ggt tct gcg gtt gta ttc gcg ggt acc acg gtg ctg atc gct 240 Thr Wing Gly Ser Wing Val Val Phe Wing Gly Thr Thr Val Leu He Wing 65 70 75 80 ctg gtt gct ctg tcg atc att aat att cca ttt cta acc gtg atg gcc 288 Leu Val Ala Leu Ser He He Asn He Pro Phe Leu Thr Val Met Wing 85 90 95 att gct gcc gc atc acc gtt gcc gc atc gtt ctg gtt gct ctg tcc 336 He Wing Wing Wing He Thr Val Wing Wing Val Leu Val Wing Leu Ser 100 105 110 tcc ctc ccc gc ctg ctt gcc ctg ctt gcc act ccc atc tcc gc gca 384 Phe Leu Pro Wing Leu Leu Gly Leu Leu Gly Thr Arg He Phe Ala Ala 115 120 1 25 cgc gtg ect gga ect aag gtt ccg gat ect gag gac gag aag cca acg 432 Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr 130 135 140. atg ggt ctg aag tgg gtc cgc ctt gtg cgc aag atg ccg gtg gct tac 480 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Wing Tyr 145 150 155 160 ctg ctg gtt ggc gtc gtt ttg ctt ggt gca atc gca att ect gcg acc 528 Leu Leu Val Gly Val Val Leu Leu Gly Wing He Wing He Pro Wing Thr 165 170 175 aat atc cgc ctg gcc atg ccg act gat ggc acc tcc acg ctg ggc acc 576 Asn Met Arg Leu Wing Met Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr • 180 185 190 gcg ccg cgc acg ggg tat gac atg acg gca gat gcg tcg gcg ccg ggc 624 Pro Wing Arg Thr Gly Tyr Asp Met Thr Wing Asp Wing Phe Gly Pro Gly 195 200 205 cgc aac gcg ccc atg att gcg ctt atc gac gca acc gac gtc ect gag 672 Arg Asn Wing Pro Met He Wing Leu He Asp Wing Thr Asp Val Pro Glu 210 215 220 gaa gag cgc cca ttg gtg ttt gga cag gcg gtg gag caa ttc ttg aac 720 10 Glu Glu Arg Pro Leu Val Phe Gly Gln Wing V Glu Gln Phe Leu Asn 225 230 235 240 act gat ggt gtg aag aat gct cag atc act ca g acc acg gag 762 • Thr Asp Gly Val Lys Asn Ala Gln He Thr Gln Thr Thr Glu 245 250 < 210 > 222 < 211 > 254 < 212 > PRT < 213 > Corynebacterium glutamicum 15 < 400 > 222 Met Thr Pro Thr Leu Wing Ser Met He Gly * Leu Wing Val Gly He Asp 1 5 10 15 Tyr Ala Leu Phe He Val Ser Arg Phe Arg Asn Glu Leu He Ser Gln 20 25 30 Thr Gly Wing Asn Asp Leu Glu Pro Lys Glu Leu Wing Glu Arg Leu Arg 35 40 45 Thr Met Pro Leu Wing Wing Arg Wing His Wing Met Gly Met Wing Val Gly 20 50 55 60 Thr Wing Gly Ser Wing Val Val Phe Wing Gly Thr Thr Val Leu He Wing 65 70 75 80 Leu Val Ala Leu Ser He He Asn lié Pro Phe Leu Thr Val Met Wing 85 90 95 < * He Ala Ala Ala He Thr Val Ala Ala Ala Ala Leu Val Ala Ala Ser 100 105. 110 Phe Leu Pro Wing Leu Leu Gly Leu Leu Gly Thr Arg He Phe Wing Wing 25 115 120 125 * * • -Wf? '° * «" ^ Arg Val Pro Gly Pro Lys Val Pro Asp Pro Glu Asp Glu Lys Pro Thr 130 135 140 Met Gly Leu Lys Trp Val Arg Leu Val Arg Lys Met Pro Val Wing Tyr 145 150 155 160 Leu Leu Val Gly Val Val Leu Leu Gly Ala He Ala Pro Ala Thr 165 170 175 Asn Met Arg Leu Ala Pro Thr Asp Gly Thr Ser Thr Leu Gly Thr 180 185 190 Wing Pro Arg Thr Gly Tyr Asp Met Thr Wing Asp Ala Phe Gly Pro Gly 195 200 205 Arg Asn Wing Pro Met He Wing Leu He Asp Wing Thr Asp Val Pro Glu 210 215 220 Glu Glu Arg Pro Leu Val Phe Gly Gln Wing Val Glu Gln Phe Leu Asn 225 230 235 240 Thr Asp Gly Val Lys Asn Wing Gln He Thr Gln Thr Thr Glu 245 250 < 210 > 223 < 211 > 393 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (370). < 223 > RXA02586 <; 400 > 223 ttctctgaga tcgtcatgat gaagtacatc gcgttcggca tgat'cgcagc gctgattctg 60 gatgccacca tcatccgcat gctgcttgtc ccccgccgtg atg.'c.ac ctg ctt cgc 115 Met His Leu Leu Arg 1 5 gac gac aac tgg tgg gca ccc ggc ttc gtt aaa aag gcc tac acc gtc 163 Asp Asp Asn Trp Trp Wing Pro Gly Phe Val Lys Lys Wing Tyr Thr Val 10 15 20 atg ggt cac ggc tct gag gag gag gaa gca ect cgc cca ac acc cgt 211 Met Gly His Gly Ser Glu Val Glu Glu Ala Pro Arg Pro Thr Thr Arg 25 30 35 cgc ctc aac gac gat gag gaa gtc gtc gtc cat gaa gca gtt gtc gct 259 Arg Leu Asn Asp Glu Glu Val Thr Val His Glu Wing Val Val Wing 40 45 50 ggc gat acc gtg gca tct cgc ggt ggt ttg age acg cag gaa aac cgt 307 Gly Asp Thr Val Wing Arg Gly Gly Leu Ser Thr Gln Glu Asn Arg 55 60 65 gat ctg gtg tcc ttc gtg gaa ctt aag gct cgt ttg gaa aag cgc agg 355 Asp Leu Val Ser Phe Val Glu Leu Lys Ala Arg Leu Glu Lys Arg Arg 70 75 80 85 ctt gag gat cta gat taaatctatg cgaggatttt tea 393 Leu Glu Asp Leu Asp 5 90 < 210 > 224 < 211 > 90 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 224 Met His Leu Leu Arg Asp Asp Asn Trp Trp Wing Pro Gly Phe Val Lys 1 5 10 15 10 Lys Wing Tyr Thr Val Met Gly His Gly Ser Glu Val Glu Wing Glu Pro 20 25 30 Arg Pro Thr Thr Arg Arg Leu Asn Asp Asp Glu Glu to Thr Val His • 35 40 45 Glu Ala Val Val Ala Gly Asp Thr Val Ala Ser Arg Gly Gly Leu Ser 50 55 60 Thr Gln Glu Asn Arg Asp Leu Val Ser Phe Val Glu Leu Lys Ala Arg 65 70 75 80 15 Leu Glu Lys Arg Arg Leu Glu Asp Leu Asp 85 90 < 210 > 225 < 211 > 2214 < 212 > DNA < 213 > Corynebacterium glutamicum • < 220 > < 221 > CDS < 222 > (101) .. (2191) 20 < 223 > RXA02587 < 400 > 225 agcctggata acctgccaga cggtggcgca tggctgcagc cgttccgccc tctgactgcc 60 ttgttatcca accgccacaa ttcccaggag taatccaccc gtg ttt tct aaa tgg 115 Val Phe Ser Lys Trp 1 5 ggc cac ttt gct tac aga ttt agg cgc att gtt ccg tta gtc gtc atc 163 Gly His Phe Wing Tyr Arg Phe Arg Arg He Val Pro Leu Val Val He 10 15 20 25 j Ügg ^^ gcc gcg att ttg gct ttg ttt gtc att ttc ggc acc aag ctg ggc gac 211 Ala Ala He Leu Ala Leu Phe Val He Phe Gly Thr Lys Leu Gly Asp 25 30 '35 cgc atg age cag gaa gga tgg gat gat ect ggt tct tcg tcg acc gct 259 Arg Met Ser Gln Glu Gly Trp Asp Asp Pro Gly Ser Ser Thr Wing 40 45 50 gcg gcg cgc atc gag ttg gag acc ttt ggg cgt gac aat gac ggc gat 307 Wing Wing Arg He Glu Leu Glu Thr Phe Gly Arg Asp Asn Asp Gly Asp 55 60 65 gtc gtg ttg ctg ttt act gcg ect gaa ggc act tct ttc gat gat gca 355 Val Val Leu Leu Phe Thr Pro Wing Glu Gly Thr Ser Phe Asp Asp Wing 70 75 80 85 gag gtg ttc tcc age atc tct ggc tac tta gat ggg cta atc gag aac 403 Glu Val Phe Ser Ser Be Gly Tyr Leu Asp Gly Leu He Glu Asn 90 95 100 aac gat gat gtc age cac atc aac age tac ttt gac act cgt aat 451 Asn Pro Asp Glu Val Ser His He Asn Ser Tyr Phe Asp Thr Arg Asn 105 110 115 caa aat ctc ctc age aaa gac ggc acc actact ttt gca gct ctc ggg 499 Gln Asn Leu Leu Ser Lys Asp Gly Thr Gln Thr Phe Wing Wing Leu Gly 120 125 130 ctc aaa ggt gac ggc gag caa acg ctg aag gac ttc cgg gag att gaa 547 Leu Lys Gly Asp Gly Glu Gln Thr Leu Lys Asp Phe Arg Glu He Glu 135 140 145 gat cag ctc cat ccg gac aac ctt gcc ggt ggc gtc ac act gag gtc 595 Asp Gln Leu His Pro Asp Asn Leu Wing Gly Gly Val Thr Thr Thu Glu Val 150 155 160 165 gcg ggt gcc gcc gcc gcc acc gct gac gcc ga ga ggc atg gct ggc 643 Ala Gly Ala Thr Ala Ala Ala Asp Ala Leu Asp Glu Gly Met Ala Gly 170 175 180 gat att tea cgc gcc gtt gtt ttt gcg ctg ect ttc gtg gct atc ttg 691 Asp He Ser Arg Ala Glu Val Phe Ala Leu Pro Phe Val Al a He Leu 185 190 195 ctc ctc atc gtg ttt ggc tea gtt gtt gcc gcg gcg atg cca ttg atc 739 Leu Leu He Val Phe Gly Ser Val Val Ala Wing Wing Met Pro Leu He 200 205 210 gtg ggc att ttg tcc atc ttg ggt tcg ctg ggc atc ttg gca att ttg 787 Val Gly He Leu Ser He Leu Gly Ser Leu Gly He Leu Wing He Leu 215 220 -225 gct gga ttc ttc cag gtc aac gta ttt gca caa tct gtt gtg acc ctt 835 Wing Gly Phe Phe Gln Val Asn Val Phe Wing Gln Ser Val Val Thr Leu 230 235 240 245 ctg ggc ttg ggt ctt gcc att gac tat ggc tta ttc atg gtc tct cgt 883 Leu Gly Leu Gly Leu Wing Asp Tyr Gly Leu Phe Met Val Ser Arg 250 255 '260 ttc cgt gag gaa atg gat aag ggc acc ccg gtt gaa cag gct gtt gcc 931 Phe Arg Glu Glu Met Asp Lys Gly Thr Pro Val Glu Gln Ala Val Ala 265 270 '. 275 r ac ac gcg ac gcg ggt aag act gtg gtg ttp tct gca gcg atg 979 Thr Thr Thr Wing Thr Wing Gly Lys Thr Val Val Pt¡e Ser Wing Wing Met 280 285 290 gtg gct gtg gcg ctg tcc ggg ttg ttt gtt ttc cca cag gct ttc ttg 1027 Val Wing Val Wing Leu Ser Gly Leu Phe Val Phe Pro Gln Wing Phe Leu 295 300 305 aag tcg gtg gca ttc ggt gcg att tcc gcg gtt ggc ctt gct gt ttg 1075 Lys Ser Val Wing Phe Gly Wing He Be Wing Val Gly Leu Ala Wing Leu 310 315 320. 325 atg tcg gtg acg gtg ttg ccg tcg ctg ttc age atg ttg ggt aag aat 1123 Met Ser Val Thr Val Leu Pro Ser Leu Phe Ser Met Leu Gly Lys Asn 330 335 340 atc gat aag tgg agt ttg cgt cgc act gct cga here gcg cgc cgt tgt 1171 He Asp Lys Trp Ser Leu Arg Arg Thr Wing Arg Thr Wing Arg Arg Leu 345 350 355 gaa gac acc att tgg tac cgc gtg ccg gca tgg gca atg cgc cat gcc 1219 Glu Asp Thr He Trp Tyr Arg Val Pro Ala Trp Wing Met Arg His Wing 360 365 370 aag gca gtg acc gtg ggc gtc gta ttg ctc ttg ctt gct ctt aca gtg 1267 Lys Wing Val Thr Val Val Val Leu Leu Leu Leu Ala Leu Thr Val 375 380 385 ccg ttg acg ggc gtg aaa ttc ggc ggc atc aat gaa acg tat ctg cca 1315 Pro Leu Thr Gly Val Lys Phe Gly Gly He Asn Glu Thr Tyr Leu Pro 390 395 400 405 cca gct aac gac acc cgc gtc gcc gcc caa gag cgt tcc gac gac gcg ttt 1363 Pro Wing Asn Thr Arg Val Wing Gln Glu Arg Phe Asp Glu Wing Phe 410 415 420 ccc gcc ttc cgc acc gag ccg gtc aag ctt gtg gtc acc ggg gcg gac 1411 Pro Wing Phe Arg Thr Glu Pro Val Lys Leu Val Val Thr Gly Wing Asp 425 430 435 aac aac cag ctg atc gat atc tat gtt cag gcc aac gaa gtt gag gga 1459 Asn Asn Gln Leu He Asp He Tyr Val Gln Wing Asn Glu Val Glu Gly 440 445 * 450 ctg aca gat cgt ttc acc ggc gcg gcg ac ac gat ggc action acc acg 1507 Leu Thr Asp Arg Phe Thr Ala Gly Wing Thr Thr Asp Asp Gly Thr Thr Thr 455 460 465 gtg ttg tct act ggt att cag gat cgt tcc ctc aat gag cag gta gtg 1555 Val Leu Be Thr Gly He Gln Asp Arg Ser Leu Asn Glu Gln Val Val 470 475 480 485 gag cag ctt cgc gct att tcc gtc ect gag ggc gtt gag gtg cag atc 1603 Glu Gln Leu Arg Ala He Ser Val Pro Glu Gly Vall Glu Val Gln He 490 495 500 ggt ggc act cca gcc atg gag atc gaa tcc att gag gcg ctc ttt gaa 1651 Gly Gly Thr Pro Wing Met Glu He Glu Be He Glu Wing Leu Phe Glu 505 510 515 aag ctc ctc tgg atg gct ctc tac att gtg ctg gcc act ttc atc ctc 1699 Lys Leu Leu Trp Met Wing Leu Tyr He Val Leu Wing Thr Phe He Leu 520 525 530 atg gca ttg gta ttt ggt tcg gtg att ttg ccg gcg aag gcc atc atc 1747 Met Ala Leu Val Phe Gly Ser Val He Leu Pro Ala Lys Ala He He 535 540 545 atg acc att ctg ggt atg ggt gcc acc ttg ggt att ctc acc ttg atg 1795 Met Thr He Leu Gly Met Gly Wing Thr Leu Gly He Leu Thr Leu Met 550 555 560 565 ttc gtc gat ggc gtg ggt gcc age gca ttg aac ttc tcc ect ggc cca 1843 Phe Val Asp Gly Val Gly Ala Be Ala Leu Asn Phe Ser Pro Gly Pro 570 575 580 ctg atg agt cca gtg ctg gtg ctg atc atg gct att att tac gga ctt 1891 Leu Met Ser Pro Val Leu Val Leu He Met Wing He He Tyr Gly Leu 585 590 595 tcc acc gac tat gag gtg ttc ctg gta tct cgc atg gtg gag gcc cgc 1939 Ser Thr Asp Tyr Glu Val Phe Leu Val Ser Arg Met Val Glu Wing Arg 600 605 '610 gat aaa ggc gaa tcc acc gac gac gcc atc aga tac ggc act gca cac 1987 Asp Lys Gly Glu Ser Thr Asp Asp Ala He Arg Tyr Gly Thr Ala His 615 620 625 acc gga tct atc atc acc gcg gcc gca ctg atc atg- att gtg gtc tgt 2035 Thr Gly Ser He Thr Wing Wing Wing Leu He Met He Val Val Cys 630 635 640 645 gga gcg ttt ggt ttc tct gag atc gtc atg atg aag tac atc gcg ttc 2083 Gly Wing Phe Gly Phe Ser Glu He Val Met Met Lys Tyr He Wing Phe 650 655 660 ggc atg atc gca gcg ctg att ctg gat gcc acc atc atc cgc atg ctg 2131 Gly Met He Ala Ala Leu He Leu Asp Ala Thr He He Arg Met Leu 665 670 675 ctt gtc ccc cgc cgt gat gca ect gct tcg cga cga caa ctg gtg ggc 2179 Leu Val Pro Arg Arg Asp Wing Pro Wing Arg Arg Gln Leu Val Gly 680 685 690 cgg ctt cgt taaaaaggcc tacaccgtca tgg 2214 Thr Arg Leu Arg 695 < 210 > 226 < 211 > 697 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 226 5 Val Phe Ser Lys Trp Gly His Phe Wing Tyr Arg Phe Arg Arg He Val 1 5 10 15 Pro Leu Val Val He Wing Wing He Leu Wing Leu Phe Val He Phe Gly 20 25 30 Thr Lys Leu Gly Asp Arg Met Ser Gin Glu Gly Trp Asp Asp Pro Gly 35 40 45 Be Ser Thr Wing Wing Wing Arg He Glu Leu Glu Thr Phe Gly Arg 50 55 60 10 Asp Asn Asp Gly Asp Val Val Leu Leu Phe Thr Wing Pro Glu Gly Thr 65 70 75 80 Being Phe Asp Asp Wing Glu Val Phe Being Ser Gly Tyr Leu Asp 85 90 95 Gly Leu He Glu Asn Asn Pro Asp Glu Val Ser His He Asn Ser Tyr 100 105 110 Phe Asp Thr Arg Asn Gln Asn Leu Leu Ser Lys Asp Gly Thr Gln Thr 115 120 125 15 Phe Wing Wing Leu Gly Leu Lys Gly Asp Gly Glu Gln Thr Leu Lys Asp 130 135 140 Phe Arg Glu He Glu Asp Gln Leu His Pro Asp Asn Leu Wing Gly Gly 145 150 • 155 160 Val Thr Thr Glu Val Ala Gly Ala Thr Ala Val Ala Asp Ala Leu Asp 165 170 175 Glu Gly Met Ala Gly Asp He Ser Arg Ala Glu Val Phe Ala Leu Pro 180 185 190 20 Phe Val Ala He Leu Leu Leu He Val Phe Gly Ser Val Val Wing Wing 195 200"205 Wing Met Pro Leu He Val Gly He Leu Ser He Leu Gly Ser Leu Gly 210 215 220 He Leu Wing He Leu Wing Gly Phe Phe Gln Val Asn Val Phe Wing Gln 225 230 235 240 Ser Val Val Thr Leu Leu Gly Leu Gly Leu Ala He Asp Tyr Gly Leu 245 250 255 25 Phe Met Val Ser Arg Phe Arg Glu Glu Met Asp Lys Gly Thr Pro Val g ^ gj 260 265 270 Glu Gln Ala Ala Ala Thr Thr Thr Ala Thr Ala Gly Lys Thr Val Val 275 280 285 Phe Ser Ala Ala Met Val Ala Ala Ala Ala Ser Gly Leu Phe Val Phe 290 295 300 Pro Gln Ala Phe Leu Lys Ser Val Ala Phe Gly Ala Be Ser Ala Ala 305 310 315 320 Gly Leu Ala Ala Leu Met Ser Val Thr Val Leu Pro Ser Leu Phe Ser 325 330 335 Met Leu Gly Lys Asn He Asp Lys Trp Ser Leu Arg Arg Thr Wing Arg 340 345 350 Thr Wing Arg Arg Leu Glu Asp Thr He Trp Tyr Arg Val Pro Wing Trp 355 360 365 Wing Met Arg His Wing Lys Wing Val Thr Val Val Val Leu Leu Leu 370 380 380 Leu Ala Leu Thr Val Pro Leu Thr Gly Val Lys Phe Gly Gly He Asn 385 390 395 400 Glu Thr Tyr Leu Pro Pro Wing Asn Asp Thr Arg Val Wing Gln Glu Arg 405 410 415 Phe Asp Glu Wing Phe Pro Wing Phe Arg Thr Glu Pro Val Lys Leu Val 420 425 430 Val Thr Gly Wing Asp Asn Asn Gln Leu He Asp He Tyr Val Gln Wing 435 440. 445 Asn Glu Val Glu Gly Leu Thr Asp Arg Phe Thr Wing Gly Wing Thr Thr 450 455 460 Asp Asp Gly Thr Thr Val Leu Ser Thr Gly He Gln Asp Arg Ser Leu 465 470 475 480 Asn Glu Gln Val Val Glu Gln Leu Arg Ala He Ser Val Pro Glu Gly 485 490 495 Val Glu Val Gln He Gly Gly Thr Pro Ala Met Glu He Glu Ser He 500 505 510 Glu Ala Leu Phe Glu Lys Leu Leu Trp Met Ala Leu Tyr He Val Leu 515 520 525 Ala Thr Phe He Leu Met Ala Leu Val Phe Gly Ser Val He Leu Pro 530 535 540 Wing Lys Wing He He Met Met Thr He Leu Gly Met Gly Wing Thr Leu Gly 545 550 555 560 He Leu Thr Leu Met Phe Val Asp Gly Val Gly Wing Being Wing Leu Asn • - «-« • 565 570 575 Phe Ser Pro Gly Pro Leu Met Pro Pro Val Leu Val Leu 'He Met Wing 580 585 590 He He Tyr Gly Leu Ser Thr Asp Tyr Glu Val Phe Leu Val Ser Arg 595 600 605 Met Val Glu Wing Arg Asp Lys Gly Glu Ser Thr Asp Asp Wing He Arg 610 615 620 Tyr Gly Thr Wing His Thr Gly Ser He He Thr Wing Wing Wing Leu He 625 630 635 640 Met He Val Val Cys Gly Wing Phe Gly Phe Ser Glu He Val Met Met 645 650 655 Lys Tyr He Wing Phe Gly Met Wing Wing Wing Leu He Leu Asp Wing Thr 660 665 670 10 He He Arg Met Leu Leu Val Pro Arg Arg Asp Wing Pro Wing Being Arg 675 680 685 Arg Gln Leu Val Gly Thr Arg Leu Arg • 690 695 < 210 > 227 < 211 > 729 < 212 > DNA < 213 > Corynebacterium glutamicum 15 < 220 > < 221 > CDS < 222 > (101) .. (706) < 223 > RXN03042 < 400 > 227 atgacaccgg cgcgacgtat ggcattactg gcgtacccca atttacgatg acatctctgc 60 • tcgcctcggc gacgtcctgg ttccttacgt tctgatcgtt ttg gtt cta gcg ttc 115 Leu Val Leu Wing Phe 1 5 20 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser He Trp Val Pro Leu He Wing 10 15 20 gct ctg ggc ttt ggc ttg tea gtt ctg gct acc ttt ggt gct acc gtg 211 Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr Phe Gly Wing Thr Val 25 30 35 gcg atc ttc caa gaa ggt gct ttc ggc atc atc gac gat ect cag cca 259 Wing He Phe Gln Glu Gly Wing Phe Gly He He Asp Asp Pro Gln Pro 40 45 50 25 ctg ctg tcc ttc ttg ccg atc atg ctc atc ggc ctg gta ttt ggt ctg 307 Leu Leu Be Phe Leu Pro He Met Leu He Gly Leu Val Phe Gly Leu 55 60 65 gcc atg gat tac cag atc ttc ctc gtt act cgt atg cgt gag ggc ttc 355 Wing Met Asp Tyr Gln He Phe Leu Val Thr Arg Met Arg Glu Gly Phe 70 75 '80 85 acc aag ggc aag act gcc ggc aac gca acg tcg aat gtc aac cac 103 Thr Lys Gly Lys Thr Wing Gly Asn Wing Thr Ser Asn Gly Phe Lys His 90 95 100 ggt gcc gcg gtg gtc act gct gcg gcg ctg atc atg gtg tct gtg ttc 451 Gly Ala Arg Val Val Thr Ala Ala Ala Leu He Met Val Ser Val Phe 105 110 - 115 gcg gca ttc ata gcg cag gac atg gcg ttt att aag acc atg ggc ttt 499 Wing Wing Phe Wing Wing Gln Asp Met Wing Wing Phe He Lys Thr Met Gly Phe 120 125 130 gct ctg gcc gtt gtg ttc ttc gat gcc ttc gtt gtt cgc atg atg 547 Wing Leu Wing Val Wing Val Phe Phe Asp Wing Phe Val Val Arg Met Met 135 140 145 att atc ect gca aca atg ttc ctg ct't gat gac aag gct tgg tgg cta 595 He He Pro Ala Thr Met Phe Leu Leu Asp Asp Lys Ala Trp Trp Leu 150 155 160 165 ect aag tgg ttg gat aag att ctt ccc aac gtt gat gtt gaa ggt gag 643 Pro Lys Trp Leu Asp Lys He Leu Pro Asn Val Asp Val Glu Gly Glu 170 175 180 ggt ctt agt gaa cta cat gag gct cgc acc gag gaa ctg aag gaa aat 691 Gly Leu Ser Glu Leu His Glu Ala Arg Thr Glu Glu Leu Lys Glu Asn 185 190 195 gta ggt gtc ggg gct tagagaaaca aaaaaggctg cta '729 Val Gly Val Gly Wing 200 < 210 > 228 < 211 > 202 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 228 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser He Trp 1 5 10 15 Val Pro Leu He Ala Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr 20 25 30 Phe Gly Wing Thr Val Wing He Phe Gln Glu Gly Wing Phe Gly He He 35 40 45 Asp Asp Pro Gln Pro Leu Leu Be Phe Leu Pro He Met Leu He Gly Leu Val Phe Gly Leu Wing Met Asp Tyr Gln He Phe Leu Val Thr Arg 65 70 75 80 Met Arg Glu Gly Phe Thr Lys Gly Lys Thr Wing Gly Asn Wing Thr Ser 85 90 95 Asn Gly Phe Lys His Gly Wing Arg Val Val Thr Wing Wing Wing Leu He 100 105 110 Met Val Ser Val Phe Wing Wing Phe He Wing Gln Asp Met Ala Phe He 115 120 125 Lys Thr Met Gly Phe Ala Leu Ala Val Ala Val Phe Phe Asp Ala Phe 130 135 140. Val Val Arg Met Met He He Pro Wing Thr Met Phe Leu Leu Asp Asp 145 150 155 160 Lys Wing Trp Trp Leu Pro Lys Trp Leu Asp Lys He Leu Pro Asn Val 165 170. ' 175 Asp Val Glu Gly Glu Gly Leu Ser Glu Leu His Glu Wing Arg Thr Glu 180 185 190 Glu Leu Lys Glu Asn Val Gly Val Gly Wing 195 200 < 210 > 229 < 211 > 729 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (706) <; 223 > FRXA02893 < 400 > 229 atgacaccgg cgcgacgtat ggcattactg gcgtacccca atttacgatg acatctctgc 60 tcgcctcggc gacgtcctgg ttccttacgt tctgccttt ttg .gtt cta gcg ttc 115 Leu Val Leu Wing Phe 1 5 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser He Trp Val Pro Leu He Wing 10 15 20 gct ctg ggc ttt ggc ttg tea gtt ctg gct acc ttt ggt gct acc gtg 211 Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr Phe Gly Wing Thr Val 25 30 35 gcg atc ttc caa gaa ggt gct tcc ggc atc atc gac gat ect cag cca 259 Wing He Phe Gln Glu Gly Wing Phe Gly He He Asp Asp Pro Gln Pro 40 45 50 ctg ctg tcc ttc ttg ccg atc atc ctc atc ggc ctg gta ttt ggt ctg 307 Leu Leu Be Phe Leu Pro He Met Leu He Gly Leu Val Phe Gly Leu 55 60 65 gcc atg gat tac cag atc ttc ctc gtt act cgt atg cgt gag ggc ttc 355 Wing Met Asp Tyr Gln He Phe Leu Val Thr Arg Met Arg Glu Gly Phe 70 75 80 ggc aag aag acc 85 act gcg ggc gca aac acg tcg aat ttc ggt aag cac 403 Thr Lys Gly Ala Gly Lys Thr Asn Ala Thr Asn Ser Gly P he Lys His 90 95 100 ggt gcc gcg gtg gtc act gcg gcg gcg ctg atc atg gtg tct gtg ttc 451 Gly Ala Arg Val Val Thr Ala Ala Ala Leu He Met Val Ser Val Phe 105 110 115 gcg gca ttc ata gcg cag gac atg gcg ttt att aag .acc atg ggc ttt 499 Wing Wing Phe Wing Wing Gln Asp Met Wing Wing Phe He Lys Thr Met Gly Phe 120 125 130 10 gct ctg gcc gtt gtg gtg ttc gtc gat gcc gt gtt gtt cgc atg atg 547 Wing Leu Wing Val Ala Val Phe Phe Asp Ala Phe Val Val Arg Met Met 135 140 145 att atc ect gca aca atg ttc ctg ctt gat gac aag gct tgg tgg cta 595 He He Pro Ala Thr Met Phe Leu Leu Asp Asp Lys Ala Trp Trp Leu 150 155 160 165 ect aag tgg t ggg aag att ctt ccc aac gtt gat gtt gag ggt gag 643 Pro Lys Trp Leu Asp Lys He Leu Pro Asn Val Asp Val Glu Gly Glu 170 175 180 15 ggt ctt agt gaa cta cat gag gct cgc acc gag aag ctg gaa gaa aat 691 Ser Leu Gly Glu Glu Ala Arg Leu His Thr Leu Lys Glu Glu Glu Asn 185 190 195 gta ggt gtc gct ggg cta tagagaaaca aaaaaggctg 729 Val Gly Val Gly Ala 200 < 210 > 230 < 211 > 202 20 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 230 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser He Trp 1 5 10 15 Val Pro Leu He Ala Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr 20 25 30 Phe Gly Wing Thr Val Wing He Phe Gln Glu Gly Ala Phe Gly He He 35 40 45 25 Asp Asp Pro Gln Pro Leu Leu Be Phe Leu Pro He Met Leu He Gly 50 55 60 Leu Val Phe Gly Leu Ala Met Asp Tyr Gln He Phe Leu Val Thr Arg 65 70 75 80 Met Arg Glu Gly Phe Thr Lys Gly Lys Thr Wing Gly Asn Wing Thr Ser 85 90 95 Asn Gly Phe Lys His Gly Wing Arg Val Val Thr Wing Wing Wing Leu He 100 105 110 Met Val Ser Val Phe Wing Wing Phe He Wing Gln Asp Met Wing Phe He 115 120 125 Lys Thr Met Gly Phe Wing Leu Wing Val Wing Val Phe Phe Asp Wing Phe 130 135 140 Val Val Arg Met Met He He Pro Al3 Thr Met Phe Leu Leu Asp Asp 145 150 155 160 Lys Wing Trp Trp Leu Pro Lys Trp Leu Asp Lys He Leu Pro Asn Val 165 170 175 Asp Val Glu Gly Glu Glu Leu Ser Glu Leu His Glu Wing Arg Thr Glu 180 185 * 190 Glu Leu Lys Glu Asn Val Gly Val Gly Wing 195 200 < 210 > 231 < 211 > 1605 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1582) < 223 > RXA01616 < 400 > 231 cctacgattt ccgacgtctt aaatcgccac acagcgccgt ggtctaaaac accaacaaaa 60 gagttgtaac tgtaccgacc attcgttaca gttacgatcc atg act tea gaa acc 115 Met Thr Ser Glu Thr 1 5 tta cag gcg caa gcg ect acg aaa acc caa cgt tgg gct ttc ctc gcc 163 Leu Gln Ala Gln Ala Pro Thr Lys Thr Gln Arg Trp Wing Phe Leu Wing 10 15 20 gtt atc age ggt ggt ctc ttt ctg atc ggt gta gac aac tcg att ctc 211 Val He Ser Gly Gly Leu Phe Leu He Gly Val Asp Asn Ser He Leu 25 30 35 tac acc gca ctc ect ctg ctg cgt gaa cag ctc gca gcc tcc gaa acc 259 Tyr Thr Ala Leu Pro Leu Leu Arg Glu Gln Leu Ala Wing Ser Glu Thr 40 45 50 caa gcg ttg tgg atc atc aac gca tat ccc ctg ctc atg gcg ggc ctt 307 Gln Ala Leu Trp He He Asn Wing Tyr Pro Leu Leu Met Wing Gly Leu 55 60 65 cgt tgc ggt gcc ggc act ttg ggt gac aaa aac ggc cac cgc cgc cgg atg 355 Arg Leu Gly Wing Gly Thr Leu Gly Asp Lys Asn Gly His Arg Arg Met 70"75 80 85 ttc ctc atg ggc ttg age att ttc gga atc gct tea ctt ggt gct gcg 403 Phe Leu Met Gly Leu Ser He Phe Gly He Wing Ser Leu Gly Wing Wing 90 • 95 100 ttt gct cca act gcg tgg gct ctt gtt gcg gcg aga gct ttc ctt ggc 451 Phe Wing Pro Thr Wing Trp Wing Leu Val Wing Wing Arg Wing Phe Leu Gly 105 110 '115 atc ggt gcg gca acg atg atg ect gca acc ttg gct ctg atc cgc att 499 He Gly Wing Wing Thr Met Met Pro Wing Thr Leu Wing Leu He Arg He 120 125 130 acg ttt gag gat gag cgt gag cgc aac act gca att ggt att tgg ggt 547 Thr Phe Glu Asp Glu Arg Glu Arg Asn Thr Wing He Gly He Trp Gly 135 140 145 tcc gtg gca att ctt ggc gct gcg gca ggc ccg atc att ggt ggt gcg 595 Ser Val Ala He Leu Gly Ala Ala Ala Gly Pro He He Gly Gly Ala 150 155 160 165 ctg ttg gaa ttc ttc tgg tgg tcg gtt ttc ctc att aac gtt ccg 643 Leu Leu Glu Phe Phe Trp Trp Gly Ser Val Phe Leu lie Asn Val Pro 170 175 180 gtg gct gtt atc gcg ttg atc gct acg ctt ttt gtg gcg ccg gcc aat 691 Val Ala Val He Ala Leu He Ala Thr Leu Phe Val Ala Pro Ala Asn 185 190 '195 atc gcg aat ccg tct aag cat tgg gat ttc tcg tcg tcc tat gcg 739 He Wing Asn Pro Ser Lys His Trp Asp Phe Leu Be Ser Phe Tyr Wing 200 205 210 ctc ctc aca ctt gct ggg ttg atc atc acg atc aag gaa tct gtg aat 787 Leu Leu Thr Leu Wing Gly Leu He He Thr He Lys Glu Ser Val Asn 215 220 225-act gca cgc cat atg ect ctt ctt ttg ggt gca gtc atc atg ttg atc 835 Thr Ala Arg His Met Pro Leu Leu Leu Gly Ala Val He Met Leu He 230 235 240 245 att ggt gcg gtg ttg ttt age agt cgt cag aag aag atc gag gag cca 883 He Gly Ala Val Leu Phe Ser Ser Arg Gln Lys Lys He Glu Glu Pro 250 255. 260 ctt cta tcg ctg tcg ttc cgt aat cgc ctt ttc tta ggc ggt gtg 931 Leu Leu Asp Leu Ser Leu Phe Arg Asn Arg Leu Phe Leu Gly Gly Val Hit f Ifit 265 270 275 gtt gcg gcg ggc atg gcg atg ttt act gtg tcc ggt ttg gaa atg act 979 Val Ala Wing Gly Met Wing Met Phe Th'r Val Ser Gly Leu Glu Met Thr 280 285 290 acc tcg cag cgt ttc cag ttg tct gtg ggt ttc act cca ctt gag gct 1027 Thr Ser Gln Arg Phe Gln Leu Ser Val Gly Phe Thr Pro Leu Glu Ala 5 295 300 305 ggt ttg ctc atg atc cca gct gca ttg ggt age tc ccg atg tct att 1075 Gly Leu Leu Met He Pro Wing Wing Leu Gly Ser Phe Pro Met Ser He 310 315 320 325 atc ggt ggt gca aac ctg cat cgt tgg ggc ttc aaa ccg ctg atc agt 1123 He Gly Gly Wing Asn Leu His Arg Trp Gly Phe Lys Pro Leu He Ser 330 335 340 ggt gtt ttt gct gcc act gcc gtt ggc atc gcc ctg tgt att tgg ggc 1171 Gly Gly Phe Ala Wing Thr Wing Val Gly He Wing Leu Cys He Trp Gly 10 345 350 355 gcg act cat act gat ggt ttg ccg ttt ttc atc gcg ggt cta ttc ttc 1219 Wing Thr His Thr Asp Gly Leu Pro Phe Phe He Wing Gly Leu Phe Phe 360 365 370 atg ggc gcg ggt gct ggt tcg gta atg tct gtg tct tcc act gcg att 1267 Met Gly Wing Gly Wing Gly Ser Val Met Ser Val Ser Ser Thr Wing He 375 380 385 atc ggt tcc gcg ccg gtg cgt aag gct ggc atg gcg tcg atc gaa 1315 He Gly Be Ala Pro Val Arg Lys Ala Gly Met Ala Be Ser He Glu 15 390 395 400 405 gag gtc tct tat gag ttc ggc acg ctg ttg tct gtc gcg att ttg ggt 1363 Glu Val Ser Tyr Glu Phe Gly Thr Leu Leu Ser Val Wing He Leu Gly 410 415 '420 age ttc ttc cca ttc ttc tac tcg ctg cat gcc ccg gca gag gtt gcg 1411 Ser Leu Phe Pro Phe Phe Tyr Ser Leu His Wing Pro Wing Glu Val Wing 425 430 435 • gat aac ttc tcg gcg ggt gtt cac gcg gcg gat gcg gat gcg gcg 1459 Asp Asn Phe Ser Wing Gly Val His His Wing Asp Gly Asp Ala Wing 20 440 445 450 cgt gc tc gt gt tc tc gc tc gtc gtg gtg gtg gtg gtg gtg gtg ct gtg Wing Being Leu Asp Thr Wing Tyr He Asn Val Leu He Wing Wing Leu 455 460 465 gta tgc gca gta gcg gct gct ctg atc age agt tac tctt tcc cgc gga 1555 Val Cys Ala Val Ala Ala Ala Leu I Ser Ser Tyr 'Leu Phe Arg Gly 470 475 480 485 aat ccg aag gga gcc aat aat gcg cac tagtaaaaaa gagatgatte 1602 Asn Pro Lys Gly Ala Asn Asn Ala His 25 490 a * - - *** • * '' - * - '- * • * tgc 1605 < 210 > 232 < 211 > 494 < 212 > PRT < 213 > Corynebacterium glutamicum 5 • < 400 > 232 Met Thr Ser Glu Thr Leu Gln Wing Gln Wing Pro Thr Lys Thr Gln Arg 1 5 10 15 Trp Wing Phe Leu Wing Val He Ser Gly Gly Leu Phe Leu He Gly Val 20 25 '30 Asp Asn Ser He Leu Tyr Thr Ala Leu Pro Leu Leu Arg Glu Gln Leu 35 40 45 Wing Wing Glu Thr Gln Wing Leu Trp He He Asn Wing Tyr Pro Leu 10 50 55 60 Leu Met Wing Gly Leu Arg Leu Gly Wing Gly Thr Leu Gly Asp Lys Asn 65 70 75 80 • Gly His Arg Arg Met Phe Leu Met Gly Leu Ser He Phe Gly He Wing 85 90 • 95 Ser Leu Gly Wing Wing Phe Wing Pro Thr Wing Trp Wing Leu Val Wing Wing 100 105 - 110 Arg Wing Phe Leu Gly He Gly Wing Wing Thr Met Met Pro Wing Thr Leu 15 115 120 125 Wing Leu He Arg He Thr Phe Glu Asp Glu Arg Glu Arg Asn Thr Wing 130 135 140 He Gly He Trp Gly Ser Val Wing He Leu Gly Wing Wing Wing Gly Pro 145 150 155 160 • He He Gly Gly Wing Leu Leu Glu Phe Phe Trp Trp Gly Ser Val Phe 165 170 175 Leu He Asn Val Val Val Ala Wing Ala Leu He Wing Thr Leu Phe 20 180 185 190 Val Wing Pro Wing Asn He Wing Asn Pro Being Lys His Trp Asp Phe Leu 195 200 205 Be Ser Phe Tyr Ala Leu Leu Thr Leu Ala Gly Leu He He Thr He 210 215 220 Lys Glu Ser Val Asn Thr Ala Arg His Met Pro Leu Leu Leu Gly Ala 225 230 235 240 Val He Met Leu He He Gly Wing Val Leu Phe Ser Being Arg Gln Lys 25 245 250 255 Lys He Glu Glu Pro Leu Leu Asp Leu Being Leu Ph? Arg Asn Arg Leu 260 265 270 Phe Leu Gly Val Val Wing Wing Gly Met Wing Met Phe Thr Val Ser 275 280 285 Gly Leu Glu Met Thr Thr Ser Gln Arg Phe Gln Leu Ser Val Gly Phe 290 295 300 • Thr Pro Leu Glu Ala Gly Leu Leu Met He Pro Ala Ala Leu Gly Ser 305 310 315 320 Phe Pro Met He He Gly Gly Wing Asn Leu His Arg Trp Gly Phe 325 330 335 Lys Pro Leu He Ser Gly Gly Phe Wing Wing Thr Wing Val Gly He Wing 340 345 350 Leu Cys He Trp Gly Wing Thr His Thr Asp Gly Leu Pro Phe Phe He 10 355 360 365 Wing Gly Leu Phe Phe Met Gly Wing Gly Wing Gly Ser Val Met Ser Val 370 375 380 • Be Ser Thr Wing He He Gly Ser Wing Pro Val Arg Lys Ala Gly Met 385 390 395 400 Wing Being Ser Glu Glu Val Ser Tyr Glu Phe Gly Thr Leu Leu Ser 405 410 415 Val Ala He Leu Gly Ser Leu Phe Pro Phe Phe Tyr Ser Leu His Wing 15 420 425 430 Pro Wing Glu Val Wing Asp Asn Phe Ser Wing Gly Val His His Wing He 435 440 4-45 Asp Gly Asp Wing Wing Arg Wing Being Leu Asp Thr Wing Tyr He Asn Val 450 455 '460 Leu He Wing Wing Leu Val Cys Ala Val Ala Ala Ala Ala Leu He Ser Ser 465 470 475 480 Tyr Leu Phe Arg Gly Asn Pro Lys Gly Wing Asn Asn Wing His 20 485 490 < 210 > 233 < 211 > 1500 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1477) < 223 > RXA01666 25 < 400 > 233 cgacgcgccc ctccaccttt tcagtagcgt cacgggcgcc aatcctgtat ttttagcagc 60 agtttgaggg tttttgctcc ccatctttag gagacacccc gtg tcc acg ttt cat 115 Val Ser Thr Phe His 1 5 aaa gtt ttg atc aac acc atg atc tcc aac gtc acc act gga ttt ctg 163 Lys Val Leu He Asn Thr Met He Be Asn Val Thr Thr Gly Phe Leu 10 15 20 ttc ttt gtg gtg gtg ttt tgg tg tg att tat ctt tcc act ggc aac gtc gca 211 Phe Phe Wing Val Val Phe Trp Met Tyr Leu Ser Thr Gly Asn Val Wing 25 30 35 ctg acc ggc atc gtc agt gga att tac atg ggt ttg atc gcc gtt tgt 259 Leu Thr Gly He Val Ser Gly He Tyr Met Gly Leu He Wing Val Cys 40 45 50 tcc atc ttt ttc gga acc gtt gtt gat cac aat cgc aag aag tcc gtc 307 Be He Phe Phe Gly Thr Val Val Asp His Asn Arg Lys Ser Val 55 60 65 atg ctg ttt age gtc acc aca ctc gtg ttt tat tgt ctc agt gcc 355 Met Leu Phe Be Ser Val Thr Thu Leu Val Phe Tyr Cys Leu Be Wing 70 75 80 85 ctg gtg tgg gtg ttt tgg ctg gag gaa gac ggc ctg age atc gga aat 403 Leu Val Trp Val Phe Trp Leu Glu Glu Asp Gly Leu Ser He Gly Asn 90 95 100 acc gcc ctg tgg gtg ttc gtt tct ttc atc ctc atc gga tea atc gtg 451 Thr Ala Leu Trp Val Phe Val Ser Phe He Leu He Gly Ser He Val 105 110 115 gaa cac atg cgc aac atc gca ctg tcc acc gtg gtc acg ctg ttg gtt 499 Glu His Met Arg Asn He Ala Leu Ser Thr Val Val Thr Leu Leu Val 120 125 • 130 ect gaa gct gaa cgc gac aaa gca aac ggc ctg gta gga gcc gtg caa 547 Pro Glu Ala Glu Arg Asp Lys Wing Asn Gly Leu Val Gly Wing Val Gln 135 140 145 ggt gtt gt gt ttt gtc gtc gtc gtc att gct gcc tcc gcc atc ggg 595 Gly Val Gly Phe Leu Val Thr Ser Val He Wing Gly Ser Wing He Gly 150 155 160 165 ttc ttg ggc atg gaa atc acc ctg tgg atc tgc ctt ggg ctc tea ctt 643 Phe Leu Gly Met Glu He Thr Leu Trp He Cys Leu Gly Leu Ser Leu 170 175 180 gtc gcg ctg ctg cac ctg ctg ccg att cgc gtc gac gaa ccg gaa atc 691 Val Ala Leu Leu His Leu Leu Pro He Arg Val Asp Glu Pro Glu He 185 190 195 atc acc gaa gac gca cag ect act gtt tct gac gat tea gtt ccc 739 He Thr Gln Glu Asp Ala Gln Pro Thr Val Ser Asp Asp Ser Val Pro 200 205 210 ac tct gat ttg gcg atc gtg tcc aaa ggc atc gac cta aaa 787 Thr Pro Thr Ser Asp Leu Ala He Val Ser Lys Gly He Asp Leu Lys 215 220 '225 gga tea atg aaa atc atc ctg agt gtt ccg gga ctg ctc gcg ctt gtg 35 Gly Ser Met Lys He He Leu Ser Val Pro Gly Leu Leu Ala Leu Val 230 235 240 245 • ttg ttt gcg tcc ttc aac aac ctc atc ggc ggc gtg tac tcc gca ctc J83 Leu Phe Wing Being Phe Asn Asn Leu He Gly Gly Val Tyr Ser- Ala Leu 250 255 260 atg gac ect tac ggc ctg gaa ctt ttc age cca cag ctg tgg ggg cta 931 Met Asp Pro Tyr Gly Leu Glu Leu Phe Ser Pro Gln Leu Trp Gly Leu 265 270 275 ctg ctt gga ctc acc age ctc ggc ttc atc gtt ggt ggt gtg atc 979 Leu Leu Gly Leu Thr Ser Leu Gly Phe He Val Gly Gly Ala Val He 10 280 285 290 tcc aaa act ggc ttg ggc aaa aac ect gtg cgc acc .ttg ctg ctg gtt 1027 Ser Lys Thr Gly Leu Gly Lys Asn Pro Val Arg Thr Leu Leu Leu Val • 295 300 305 'aat gtt gtg gtg gtt ttt gtt ggc atg tta ttt gcc att cgc gaa tgg 1075 Asn Val Gly Val Wing Phe Val Gly Met Leu Phe Wing He Arg Glu Trp 310 315 320 325 tgg tgg ctc tac atc ctg ggc att ttc atc tc atc atc acccca 1123 Trp Trp Leu Tyr He Leu Gly He Phe He Phe Met Wing He Thr Pro 15 330 335 340 gcc gaa gcc gca gaa caa acc atc ctt caa cga gtc gtc cca tcc 1171 Ala Ala Glu Ala Ala Gllu Gln Thr He Leu Gln Arg Val Val Pro Phe 345 350 355 cgc caa caa cag ggc cgc gta ttt gga cta gcc atg gca gtg gaa atg gca 1219 Arg Gln Gln Gly Arg Val Phe Gly Leu Ala Met Wing Val Glu Met Wing • 360 365 370 gcc aac ccc ctc tcc aca gtg atc gtg gcg att ttg gcc gaa g cc tac 1267 Wing Asn Pro Leu Ser Thr Val He Val Wing He Leu Wing Glu Wing Tyr 20 375 380 385 ctc att cca tgg atg ggc ccc ggc gcg gac acc atc tgg ggc gtg 1315 Leu He Pro Trp Met Wing Gly Pro Gly Ala Asp Thr He Trp Gly Val 390 395 400 405 atc ctc ggc gag ggt aaa cgc ggc atg gca ctg atg ttc ctc gca 1363 He Leu Gly Glu Gly Lys Wing Arg Gly Met Wing Ala Leu Met Phe Leu Wing 410 415 420 tea ggt gcc atc atg ttg gtt gtc gtg ctg ttg gca ttc atg tcg agg 1411 Ser Gly Ala He Met Leu Val Val Val Leu Leu Ala Phe Met Ser Arg 25 425 430 435 ^ t ^ ti ± - tcc tac cgg aaa ctc age cag tac tac gcc acc acc age caa gac att 1459 Ser Tyr Arg Lys Leu Ser Gln Tyr Tyr Ala Thr Thr Ser Gln Asp He 440 445 '450 gcg gga gag aag taagtt agaccgttgt ttg 1500 Ala Gly Ala Ala Glu Lys 455 »< 210 > 234 < 211 > 459 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 234 Val Ser Thr Phe His Lys Val Leu He Asn Thr Met He Ser Asn Val 1 5 10 15 Thr Thr Gly Phe Leu Phe Phe Wing Val Val Phe Trp Met Tyr Leu Ser 10 20 25 30 Thr Gly Asn Val Ala Leu Thr Gly He Val Ser Gly He Tyr Met Gly 35 40 45 • Leu He Wing Val Cys Ser He Phe Phe Gly Thr Val Val Asp His Asn 50 55 60 Arg Lys Ser Val Met Leu Phe Ser Ser Val Thr Thr Leu Val Phe 65 70 75 80 Tyr Cys Leu Ser Ala Leu Val Trp Val Phe Trp Leu Glu Glu Asp Gly 15 85 90 95 Leu Ser He Gly Asn Thr Ala Leu Trp Val Phe Val Ser Phe He Leu 100 105 110 He Gly Ser He Val Glu His Met Arg Asn He Wing Leu Ser Thr Val 115 120 125 Val Thr Leu Leu Val Pro Glu Wing Glu Arg Asp Lys Wing Asn Gly Leu 130 135 140 Val Gly Wing Val Gln Gly Val Gly Phe Leu Val Thr Ser Val He Wing 20 145 150 155 160 Gly Ser Wing He Gly Phe Leu Gly Met Glu He Thr Leu Trp He Cys 165 170 175 Leu Gly Leu Ser Leu Val Wing Leu Leu His Leu Pro He Arg Val 180 185 190 Asp Glu Pro Glu He He Thr Gln Glu Asp Wing Gin Pro Thr Val Ser 195 200 205 Asp Asp Ser Val Pro Thr Pro Thr Ser Asp Leu Wing He Val Ser Lys 25 210 215 220 Gly He Asp Leu Lys Gly Ser Met Lys He He Leu Ser Val Pro Gly 225 230 235 240 Leu Leu Ala Leu Val Leu Phe Wing Being Phe Asn Asn Leu He Gly Gly 245 250 255 Val Tyr Be Ala Leu Met Asp Pro Tyr Gly Leu Glu Leu Phe Ser Pro 260 265 270 • Gln Leu Trp Gly Leu Leu Leu Gly Leu Thr Ser Leu Gly Phe He Val 275 280 285 Gly Gly Wing Val He Ser Lys Thr Gly Leu Gly Lys Asn Pro Val Arg 290 295 300 Thr Leu Leu Leu Val Asn Val Gly Val Wing Phe Val Gly Met Leu Phe 305 310 315 320 Ala He Arg Glu Trp Trp Trp Leu Tyr He Leu Gl'y He Phe He Phe 10 325 330 '335 Met Wing He Thr Pro Wing Wing Glu Wing Wing Glu Gln Thr He Leu Gln 340 345 350 Arg Val Val Pro Phe Arg Gln Gln Gly Arg Val Phe Gly Leu Ala Met 355 360 365 Wing Val Glu Met Wing Ala Asn Pro Leu Ser Thr Val He Val Wing He 370 375 380 Leu Wing Glu Wing Tyr Leu He Pro Trp Met Wing Gly Pro Gly Wing Asp 15 385 390 395 400 Thr He Trp Gly Val He Leu Gly Glu Gly Lys Wing Arg Gly Met Wing 405 410 415 Leu Met Phe Leu Wing Ser Gly Wing He Met Leu Val Val Val Leu 420 425 430 Wing Phe Met Ser Arg Ser Tyr Arg Lys Leu Ser Gln Tyr Tyr Ala Thr 435 440 445 Thr Ser Gln Asp He Wing Gly Wing Wing Glu Lys 20 450 455 < 210 > 235 < 211 > 1521 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1498) < 223 > RXA00062 25 < 400 > 235 cttcaacata ggcgttgggg ctgactttta a'acaggtacc agtagtaccg gcataagcga 60 tcactgttgc gttttcttgc tgccatcaaa aattagtcac atg att tta age atc 115 Met He Leu Ser He 1 5 gtc ctt ttg ggc tac ttc atg att ctg ctt gac acc tcc atc gtc att 163 5 Val Leu Leu Gly Tyr Phe Met He Leu Leu Asp Thr Ser He Val He * 10 15 20 acg ggt cta ect gcc atc ggc agt gaa ctt ggc atc gat ccc gtg cac 211 Thr Gly Leu Pro Ala He Gly Ser Glu Leu Gly He Asp Pro Val His 25 30 35 ctg tea tgg gtg cag agt tcc tac acatta gtc ttc ggc gca ctt ctt 259 Leu Ser Trp Val Gln Ser Ser Tyr Thr Leu Val Phe Gly Ala Leu Leu 40 45 50 ctg ctg gga gct cgt gcc gt gat gtc gtc cg aga cgt aaa gtg ctc 307 10 Leu Leu Gly Ala Arg Ala Gly Asp He Phe Gly Arg Lys Lys Val Leu 55 60 65 tac att ggt ctc gcg ttg ttt gcg gct tea tcg ttg gca att gcg ctt 355 Tyr He Gly Leu Ala Leu Phe Ala Wing Be Ser Leu Ala Be Ala Leu 70 75 80 85 tct cca aat gct gcg gtc ctc att gga gca cgc gta gtt cag ggc gcg 403 Ser Pro Asn Ala Ala Val Leu He Gly Ala Arg Val Val Gln Gly Wing 90 95 100 gga gct gcg att atc gcc cc gcg aca ctt gcg ttg att act gac ttc 451 15 Gly Wing Wing He He Wing Pro Wing Thr Leu Wing Leu He Thr Glu Phe 105 110 115 ttc ccc gaa ggc cca gct cgc ctt cgt gct acc tct gct tat ggt gct 499 Phe Pro Glu Gly Pro Wing Arg Leu Arg Wing Thr Ser Wing Tyr Gly Wing 120 125 '130 gtt gcc ggc atc ggt gtg gca gca ggc cta gtg atc ggc ggc gta ttt 547 Val Wing Gly He Gly Val Wing Wing Gly Leu Val He Gly Gly Val Phe 135 140 145 gct gat ctt ttg tcg tgg cgc atc ggc ttc ttt atc aac gtc ccc atc 595 20 Wing Asp Leu Leu Ser Trp Arg He Gly Phe Phe He Asn Val Pro He 150 155 160 _ 165 gcc gca gtg ttg gcc tac ata gtg cac aaa gcc att ccc gca acc ttc 643 Wing Wing Val Leu Wing Tyr He Val His Lys Wing Pro Pro Wing Thr Phe 170 175 180 age agg ect gga tea ctc gac atc ttc gga gca att "acc tcc acg gca 691 Ser Arg Pro Gly Ser Leu Asp He Phe Gly Wing He Thr Ser Thr Wing 185 190 195 ggt atc gcc gcg gtg ctc tac gca att gtc cgc age gcc gat tac age 739 25 Gly He Ala Ala Val Leu Tyr Ala He Val Arg Ser Wing Asp Tyr Ser 200 205 210 tgg aca gat ccg ttt gtg ttg att tcc ctc gtg ctg ggc atc gca gtg 787 Trp Thr Asp Pro Phe Val Leu He Ser Leu Val Leu Gly He Ala Val 215 220 225 ttc atc tgg ttc ctg cgc cat gaa tcc tea gcc aaa gaa cca ctt ctg 835 Phe He Trp Phe Leu Arg His Glu Ser Be Ala Lys Glu Pro Leu Leu 230 235 240 245 # ccc ctg ggg ctc ttt aaa aac cgc agg cga aac acc atc tcg gcc age 883 Pro Leu Gly Leu Phe Lys Asn Arg Arg Arg Asn Thr He Leu Wing Ser 250 255 260 cgc ttt ctt ctg gtt ggc tc gtg atg tea ttc ttc ttc ttt gcc acc 931 Arg Phe Leu Leu Val Gly Ser Val Met Ser Phe Phe Phe Phe Ala Thr 265 270 275 cag ctg ttc cag gac acc atg gga atg aat gct ctc cag gca ggc ctt 979 Gln Leu Phe Gln Asp Thr Met Gly Met Asn Ala Leu Gln Ala Gly Leu 10 280 285 290 gcg ttc atg ccg cta tct ctg ctg cag ttt gcc age gcc gcg atg gtg 1027 Wing Phe Met Pro Leu Ser Leu Leu Gln Phe Wing Be Ala Wing Met Val 295 300 305. cca cgg ctt tcc cga gca ggc gta tct gat tcc atg ctc acc gtc atc 1075 Pro Arg Leu Ser Arg Wing Gly Val Ser Asp Ser Met Leu Thr Val He 310 315 320, 325 ggt ttc gcc atc atg gtc atc ggc atg gca ggc ctc gca ttt gta cca 1123 Gly Phe Wing He Met Val He Gly Met Wing Gly Leu Wing Phe Val Pro 15 330 335 340 aac acg atg atc gcg ctg atc cta cca ata gtt ttg gtg gga ttt ggc 1171 Asn Thr Met He Wing Leu He Leu Pro He Val Leu Val Gly Phe Gly 345 350 355 caa ggc ttc gct ttc gga cca atg aca gct ctg gca gtt caa ggt gca 1219 Gln Gly Phe Ala Phe Gly Pro Met Thr Ala Leu Wing Val Gln Gly Wing 360 365 370 # ccg aag gac caa tcc ggc gcc gtt tct ggc ctg gtg aat tcc ctt cac 1267 Pro Lys Asp Gln Ser Gly Wing Val Ser Gly Leu Val Asn Ser Leu His 20 375 380 385 caa atc ggc ggc acc ttc ggt tg gtg gtg ttc tcc tcc tcg gct gcc 1315 Gln He Gly Gly Thr Phe Gly Leu Gly Val Phe Ser Ser Leu Wing Val 390 395 400 405 gct gtc atc gga cat gat gca aca tea gag atg atc age gac cgc gca 1363 Wing Val He Gly His Asp Wing Thr Ser Glu Met He Ser Asp Arg Wing 410 415 420 cac ttc gtc ttc ttc ctc tcc acc gtg acg ctg acg ctg gcc acc atc 1411 His Phe Gly Phe Leu Leu Ser Thr Val T hr Leu Thr 'Leu Wing Thr He 25 425 430 435 ttt gcg gtc aca ctg ctg aag cgc cac gaa acc acga ag ag age age gag 1459 Phe Ala Val Thr Leu Leu Lys Arg His Glu Thr Arg Lys Ser Ser Glu 440 445 450 cgc cca acc cag ctc gtc gac gaa aag gca gtt acc tct tagtgcgctg 1508 Arg Pro Thr Gln Leu Val Asp Glu Lys Wing Val Thr Ser 455 460 465 cagcatccca gtt 1521 < 210 > 236 < 211 > 466 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 236 Met He Leu Ser He Val Leu Leu Gly Tyr Phe Met He Leu Leu Asp 1 5 10 15 Thr Ser He Val He Thr Gly Leu Pro Wing He Gly Ser Glu Leu Gly 20 25 30 He Asp Pro Val His Leu Ser Trp Val Gln Being Ser Tyr Thr Leu Val 35 40 45 Phe Gly Ala Leu Leu Leu Leu Gly Ala Arg Ala Gly Asp He Phe Gly 50 55 60 Arg Lys Lys Val Leu Tyr He Gly Leu Ala Leu Phe Ala Wing Being Ser 65 70 75,; 80 Leu Ala He Ala Ala Leu Ser Pro Asn Ala Ala Val Leu He Gly Ala Arg 85 90 95 Val Val Gln Gly Ala Gly Ala Ala He He Ala Pro Ala Thr Leu Ala 100 105 110 Leu He Thr Glu Phe Phe Pro Glu Gly Pro Ala Arg Leu Arg Ala Thr 115 120 1.25 Be Wing Tyr Gly Wing Val Wing Gly He Gly Val Wing Wing Gly Leu Val 130 135 140 He Gly Gly Val Phe Wing Asp Leu Leu Ser Trp Arg He Gly Phe Phe 145 150 155 160 He Asn Val Pro He Wing Wing Val Leu Wing Tyr He Val His Lys Wing 165 170 175 He Pro Wing Thr Phe Ser Arg Pro Gly Ser Leu Asp. He Phe Gly Wing 180 185 190 He Thr Ser Thr Wing Gly He Wing Wing Val Leu Tyr Wing He Val Arg 195 200 - 205 Ser Wing Asp Tyr Ser Trp Thr Asp Pro Phe Val Leu He Ser Leu Val 210 215 220 Leu Gly He Wing Val Phe He Trp Phe Leu Arg His Glu Ser Ser Ala 225 230 235 240 Lys Glu Pro Leu Leu Pro Leu Gly Leu Phe Lys Asn Arg Arg Arg Asn 245 250 255 Thr He Leu Wing Being Arg Phe Leu Leu Val Gly Ser Val Met Ser Phe 260 265 270 Phe Phe Phe Wing Thr Gln Leu Phe Gln Asp Thr Met Gly Met Asn Wing 275 280 '285 Leu Gln Wing Gly Leu Wing Phe Met Pro Leu Ser Leu Leu Gln Phe Wing 290 295 300 Wing Wing Met Val Pro Arg Leu Ser Arg Wing Gly Val Ser Asp Ser 305 310 315 320 Met Leu Thr Val He Gly Phe Wing He Met Val He Gly Met Wing Gly 325 330 335 Leu Wing Phe Val Pro Asn Thr Met He Wing Leu He Leu Pro He Val 340 345 350 Leu Val Gly Phe Gly Gln Gly Phe Wing Phe Gly Pro Met Thr Ala Leu 355 360 365 Wing Val Gln Gly Ala Pro Lys Asp Gln Ser Gly Ala, Val Ser Gly Leu 370 375 38? ' Val Asn Ser Leu His Gln He Gly Gly Thr Phe Gly Leu Gly Val Phe 385 390 395 400 Be Ser Leu Wing Val Wing Val He Gly His Asp Wing Thr Ser Glu Met 405 410 '415 He Ser Asp Arg Wing His Phe Gly Phe Leu Leu Ser Thr Val Thr Leu 420 425. • 430 Thr Leu Wing Thr He Phe Wing Val Thr Leu Leu Lys Arg His Glu Thr 435 440 445 Arg Lys Ser Ser Glu Arg Pro Thr Gln Leu Val Asp Glu Lys Wing Val 450 455 '460. Thr Ser 465 < 210 > 237 < 211 > 1584 < 212 > DNA < 213 > Corynebacterium glutamicum - "-" '* • * - - * j ^^^^ < 220 > < 221 > CDS < 222 > (101) .. (1561) < 223 > RXA00215 < 400 > 237 cagtgcaaac tgaccccgca tcctaaaccg cgccagattt ctacctcaaa gaattgaagg 60 ccttttccag gcgccctcgt gcgtgaaaga ataactcaac gtg tct gac aaa aag 115 Val Ser Asp Lys Lys 1 5 cag gat cta aca tcc tcc gca gca ggt agt gct gca ccc caa acc aag 163 Gln Asp Leu Thr Ser Ser Ala Ala Gly Be Ala Ala Pro Gln Thr Lys 10 15 20 gcc tac ccc gcc atg ccc ttg ect gaa aag caa gct tgg cca gct cta 211 Wing Tyr Pro Wing Met Pro Leu Pro Glu Lys Gln Wing Trp Pro Wing Leu 25 30 _ 35 att gcc ttg tgc att ggg ttt ttc atg atc ctg ttg gat caa acc atc 259 Wing Wing Leu Cys He Gly Phe Phe Met He Leu Leu Asp Gln Thr He 40 45 50 gtg gcc gtc tct acc cca gcg tta cag gca gac atg ggc gcg tcc tac 307 Val Ala Val Ser Thr Pro Ala Leu Gln Ala Asp Met Gly Ala Ser Tyr 55 60 65 aac gag gtc atc tgg gta acc tcg gtg tat ctc ctc act tcc gcg gtg 355 Asn Glu Val He Trp Val Thr Ser Val Tyr Leu Leu 'Thr Phe Wing Val 70 75 80 85 cca ctg ctt gtt act ggc cgt tgc ggc gac aag tac ggt ccg aaa aat 403 Pro Leu Leu Val Thr Gly Arg Leu Gly Asp Lys Tyr Gly Pro Lys Asn 90 95 100 gtc tat gtc gca ggc atg gtt atc ttc aca gtg age tct ttg gcc tgt 451 Val Tyr Val Wing Gly Met Val He Phe Thr Val Ser Ser Leu Wing Cys 105 110 '115 ggt ttg gcc cca gac atg ttc acg ttg att atc gct cgt ggc gtt caa 499 Gly Leu Wing Pro Asp Met Phe Thr Leu He He Wing Arg Gly Val Gln 120 125 130 ggt tg ggc gc gc ctt ttg act cca caa acc atg gca aca atc aac 547 Gly Leu Gly Ala Wing Leu Leu Thr Pro Gln Thr Met Wing Thr He Asn 135 140 145 cgc atc ttt gct ttt gag cgc cgc ggt gca gct ctt gga gtg tgg ggt 595 Arg He Phe Wing Phe Glu Arg Arg Gly Wing Wing Leu Gly Val Trp Gly 150 155 160 165 tct aca gct ggc ctt gca tcc cta gca gga ccg atc ctg ggt ggt gtc 643 Ser Thr Wing Gly Leu Wing Ser Leu Wing Gly Pro He Leu Gly Gly Val 170 175 180 atc acc gaa aac tgg ggt tgg caa tgg gtc ttc tac atc aac gtg ccc 691 He Thr Glu Asn Trp Gly Trp Gln Trp Val Phe Tyr He Asn Val Pr 185 190 •. 195 atc ggc gtg atc tcg gtg atc gca gta atg aag tac gtt ect gaa ttc 739 He Gly Val He Ser Val He Wing Val Met Lys Tyr Val Pro Glu Phe 200 205 210 cca ccg ctg acc cga ccg ctt gat ccg ctt tct atc gtg ttg tcc atc 787 Pro Pro Leu Thr Arg Pro Leu Asp Pro Leu Ser He Val Leu Ser He 215 220 225 gtg gcc gtg ttc ttc ctg gtg ttt gct ttc cag gaa ggc gaa ggc gct 835 Val Val Val Phe Phe Leu Val Phe Ala Phe Gln Glu Gly Glu Gly Wing 230 235 240 245 ggc tgg gcg gca tgg gtg tgg atc atg atc gta gcc gcc gtc ctc ctc 883 Gly Trp Wing Wing Trp Val Trp He Met He Val Wing Wing Phe Ala Leu 250 255 260 ttt gcg tgg ttt atc tac caa age agg gcc gag aaa tcc gga aac 931 Phe Wing Trp Phe He Tyr Gln Gln Ser Arg Wing Glu Lys Ser Gly Asn 265 270 275 gat ect ctc gtc cca ctg gag att ttc aag ttt aga aac ttc age ctc 979 Asp Pro Leu Val Pro Leu Glu He Phe Lys Phe Arg Asn Phe Ser Leu 280 285 290 ggc aat atc tgc atc gcc atg gga ttc acc gtg gct gct act ect 1027 Gly Asn He Cys He Met Wing Met Gly Phe Thr Val Wing Gly Thr Pro 29 5 300 305 ctg ccc atc atg ttg tac tgc cag caca gca cac gga atg aac gcc atg 1075 Leu Pro He Met Leu Tyr Phe Gln Gln Wing His Gly Met Asn Wing Met 310 315 320 325 gaa gcg ggt ttc atg atg gtg ect caa gct ctc atg gca gta gta ctg 1123 Glu Wing Gly Phe Met Met Val Pro Gln Wing Leu Met Wing Wing Val Leu 330 335 340 tea cca ttt gtt gga aag ctg gtt gat cga tcc aac ect gga ctc atg 1171 Ser Pro Phe Val Gly Lys Leu Val Asp Arg Ser Asn Pro Gly Leu Met 345 350 355 gca gcc ctc ggt ttt age aca gtg gct gtg tcc att gta ctg ctg tea 1219 Ala Ala Leu Gly Phe Ser Thr Val Ala Val Ser He Val Leu Leu Ser 360 365 370 atg gta atg att ttc gat acg ggt cta gtc tgg gca ctt gtt tcg atg 1267 Met Val Met He Phe Asp Thr Gly Leu Val Trp Ala Leu Val Ser Met 375 380 385 act ttg ctc ggc atc gga aac gcc ttt gtg tgg gca ccg aac tcg acc 1315 Thr Leu Leu Gly He Gly Asn Wing Phe Val Trp Wing Pro Asn Ser Thr 390 395 400 • 405 tcc act atg cgc gac ctg cca cac aag ttc atg gga gcg ggc tct ggc 1363 Ser Thr Met Arg Asp Leu Pro His Lys Phe Met Gly Wing Gly Ser Gly 410 415 420 gtg ttc aat aca acc cgc caa tta ggt tea gtc ate ggc gcc gcc gcc gcc 1411 Val Phe Asn Thr Thr Arg Gln Leu Gly Ser Val He Gly Wing Wing Wing 425 430 435 atc ggc gcg gta atg cag att cga cg gca gca ggc gat gag ggc gca 1459 He Gly Wing Val Met Gln He Arg Leu Wing Wing Gly Asp Glu Gly Wing 440 445 450 gct ttt ggt caa gca ctt cta ctt gcc gct gcg gtg ctg gtt atc ggc 1507 Wing Phe Gly Gln Ala Leu Leu Leu Ala Ala Ala Ala Val Leu Val He Gly 455 460 465 att gtg gca tea acg atg gca gga aaa aat gca cac cca gcg ccg gta 1555 He Val Ala Ser Thr Met Ala Gly Lys Asn Ala His Pro Ala Pro Val 470 475 480 485 aag ect taaaggtcgc atgaatcctt cga 1584 Lys Pro < 210 > 238 < 211 > 487 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 238 Val Ser Asp Lys Lys Gln Asp Leu Thr Ser Be Wing Wing Gly Be Wing 1 5 10 15 Wing Pro Gln Thr Lys Wing Tyr Pro Wing Met Pro Leu 'Pro Glu Lys Gln 20 25 •' 30 Wing Trp Pro Wing Leu He Wing Leu Cys He Gly Phe Phe Met He Leu 35 40 45 Leu Asp Gln Thr He Val Wing Val Ser Thr Pro Wing Leu Gln Wing Asp 50 55 60 Met Gly Wing Ser Tyr Asn Glu Val He Trp Val Thr Ser Val Tyr Leu 65 70 75 80 Leu Thr Phe Ala Val Pro Leu Leu Val Thr Gly Arg Leu Gly Asp Lys 85 90 95 Tyr Gly Pro Lys Asn Val Tyr Val Wing Gly Met Val He Phe Thr Val 100 105 110 Ser Ser Leu Ala Cys Gly Leu Ala Pro Asp Met Phe Thr Leu He He 115 120 125 Wing Arg Gly Val Gln Gly Leu Gly Wing Wing Leu Leu Thr Pro Gln Thr 130 135 140 Met Wing Thr He Asn Arg He Phe Wing Phe Glu Arg Arg Gly Wing Wing 145 150 155 160 Leu Gly Val Trp Gly Be Thr Wing Gly Leu Wing Ser Leu Wing Gly Pro 165 170 175 He Leu Gly Gly Val He Thr Glu Asn Trp Gly Trp Gln Trp Val Phe 180 185 190 Tyr He Asn Val Pro He Gly Val He Ser Val He Wing Val Met Lys 195 200 205 Tyr Val Pro Glu Phe Pro Pro Leu Thr Arg Pro Leu Asp Pro Leu Ser 210 215 220 He Val Leu Ser He Val Val Wing Phe Phe Leu Val Phe Wing Phe Gln 225 230 235 240 Glu Gly Glu Gly Wing Gly Trp Wing Wing Trp Val Trp He Met He Val 245 250 255 Wing Wing Phe Wing Leu Phe Wing Trp Phe He Tyr Gln Gln Ser Arg Wing 260 265 270 Glu Lys Ser Gly Asn Asp Pro Leu Val Pro Leu Glu He Phe Lys Phe 275 280 285 Arg Asn Phe Ser Leu Gly Asn He Cys He Met Wing Met Gly Phe Thr 290 295 300 Val Wing Gly Thr Pro Leu Pro He Met Leu Tyr Phe Gln Gln Wing His 305 310 315 320 Gly Met Asn Ala Met Glu Wing Gly Phe Met Met Val Pro Gln Wing Leu 325 330 335 Met Ala Ala Val Leu Ser Pro Phe Val Gly Lys Leu Val Asp Arg Ser 340 345 350 Asn Pro Gly Leu Met Ala Ala Leu Gly Phe Ser Thr Val Ala Val Ser 355 360 365 He Val Leu Leu Ser Met Val Met He Phe Asp Thr Gly Leu Val Trp 370 375 380 Wing Leu Val Ser Met Thr Leu Leu Gly He Gly Asn Wing Phe Val Trp 385 390 395 400 Wing Pro Asn Ser Thr Ser Thr Met Arg Asp Leu Pro His Lys Phe Met 405 410 415 Gly Wing Gly Ser Gly Val Phe Asn Thr Thr Arg Gln Leu Gly Ser Val 420 425 430 He Gly Wing Wing Wing He Gly Wing Val Met Gln He Arg Leu Wing Wing 435 440 445 Gly Asp Glu Gly Wing Wing Phe Gly Gln Wing Leu Leu Leu Ala Ala Ala 450 455 460 Val Leu Val He Gly He Val Ala Ser Thr Met Ala Gly Lys Asn Ala 465 470 475 480 His Pro Ala Pro Val Lys Pro 485 < 210 > 239 < 211 > 1455 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1432) < 223 > RXN03064 < 400 > 239 10 tggagccttg tcttcctcca gcaatcccac aacggagcag gttgggatcc cgagaaatgt 60 tgtcatcatc ttggctgtat tagtttttac agcctttgtc atg atg ttg aat gag 115 Met Met Leu Asn Glu 1 5 act act ctg gca gtc gcg ttg ccg tcg atc atg gcg gac ttt gac att 163 Thr Thr Leu Ala Val Ala Leu Pro Ser He Met Wing Asp Phe Asp He 10 15 20 gag gcg aat act gcg cag tgg ttg ctc act ggt ttt atg ttg acc atg 211 Glu Wing Asn Thr Wing Gln Trp Leu Leu Thr Gly Phe Met Leu Thr Met 15 25 30 35 gct gtg gtt ctt cct gct act ggt tgg atg ttg gaa cgt ttt acc act 259 Wing Val Val Leu Pro Wing Thr Gly Trp Met Leu Glu Arg Phe Thr Thr 40 45 50 cgt agt gtg ttt att ttc gcc acg gtg gtc ttc ctg atc ggt act gtg 307 Arg Ser Val Phe He Phe Wing Thr Val Val Phe Leu He Gly Thr Val 55 60 65 acg gct gcg tctgctctctcttttttcc att atg ctt gcc gc cgc gtc 355 Thr Ala Ala Leu Ser Pro Thr Phe Ala He Met Leu Wing Wing Arg Val 20 70 75 80 85 gct cag gcg att ggt acc gct gtg atc atc ccg ctg atg act gtc 403 Ala Gln Ala He Gly Thr Ala Val He M et Pro Leu Leu Met Thr Val 90 95 100 gcg atg acc gtt ect cca gag cgc cgt ggc gcc gtc atg ggt ttg 451 Wing Met Thr Val Val Pro Pro Glu Arg Arg Gly Ala Val Met Gly Leu 105 110 115 att gcg gtc gtg atg gcc gtt ggt ect gct ctt gga ect agt gtg gct 499 He Wing Val Val Wing Wing Val Gly Pro Wing Leu Gly Pro Ser Val Wing 25 120 125 130 - ^ < j, e £ m < BtMiMaM - J ^ M ' - - - - - - ggt ttc gta ctc age ttg tct tcg tgg cac gcg att ttc tgg gtc atg 547 Gly Phe Val Leu Ser Leu Ser Ser Trp His Wing He Phe Trp Val Met 135 140 145 gtt ccg ttg gtg ttt gtg gca age ctg atc ggt acc ctg cgt ctg acc 595 Val Pro Leu Val Phe Val Wing Ser Leu He Gly Thr Leu Arg Leu Thr 150 155 160 165 aac gtc agt gag ect aaa aag act ect ttg gat gtt att tcc ttc ctg 643 Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp Val He Ser Phe Leu 170 175 180 att tcc gca gtg gct ttc ggt ggc ctt gtg tac gcc ttg age tcg att 691 He Ser Ala Ala Ala Phe Gly Gly Leu Val Tyr Ala Leu Ser Ser He 185 190 195 ggc atc att ttg gaa ggt gac aga age gct ttg gtc gtg ttg gct gtc 739 Gly He He Leu Glu Gly Asp Arg Ser Ala Leu Val Val Leu Ala Val 200 205 210 10 ggc atc att gcg ttg gtg gtg ttt gtg tgg cgc cag att gcc atg ggt 787 Gly He He Ala Ala Val Val Phe Val Trp Arg Gln He Ala Met Gly 215 220 '225 aag cag gat aag gcg ctg ttg gat ctg cgt ccg ttg gcg att cgt gag 835 Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg P ro Leu Ala He Arg Glu 230 235 240 245 tac acc att ccg ctg gtt gtg ctt ttg acg ctg ttc ggt gcg ctg ctc 883 Tyr Thr He Pro Leu Val Val Leu Leu Thr Leu Phe Gly Ala Leu Leu 250 255 260 15 ggt gtc atg aat aca ctg ccg ctc tac ctg cag gga tcc ttg atg gtc 931 Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln Gly Ser Leu Met Val 265 270 275 acc gcc ttg gtc gcg ggt cta gtg ctg ttg cca ggt ggt ctt ttg gaa 979 Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro Gly Gly Leu Leu Glu 280 285 290 ggt gtg ctg tcg cca ttt gtg ggt cga att tat gat cgt cat ggt cca 1027 Gly Val Leu Ser Pro Phe Val Gly Arg He Tyr Asp Arg His Gly Pro 295 300 305 20 cgc gga ctc gtg atc ggc ggt atg tea ctc gtt gtg atc tcc ctg ttt 1075 Arg Gly Leu Val He Gly Gly Met Ser Leu Val Val He Ser Leu Phe 310 315 320 325 gca ctg tcc acc gtc gat gag ttc gcc aac gtg tgg ttc atc atc ggc 1123 Wing Leu Ser Thr Val Asp Glu Phe Wing Asn Val Trp Phe He He Gly 330 335 340 gta cac atc gtg ttc tcc atc ggc ctt gcg ctg ctg ttc acc cca ctg 1171 Val Hi s He Val Phe Ser He Gly Leu Ala Leu Leu Phe Thr Pro Leu 345 350 355 25 ÜHMMHfaaSMaa. < - r.,. . ..- ».. .. atg aca gtc gcg ctc gca tcc gtc ccc gac aac atg tac ggc cac ggc 1219 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly 360 365 370cc gcg atc ctc aac acc ctc caa cag ctc gcc ggc gcc gcc ggc acc 1267 Be Ala He Leu Asn Thr Leu Gln Gln Leu Wing Gly Wing Wing Gly Thr 375 380 385 gcg gtc atg att gcg gtt tat tcc acc gtc age aac aac gcg ctt atc 1315 Ala Val Met He Ala Val Tyr Ser Thr Val Ser Asn Asn Wing Leu He 390 395 400, 405 gac ggc gca acca caa acc gcc ccc gcc gac ggc gcc aac tct gca 1363 Asp Gly Ala Thr Gln Gln Thr Ala Leu Wing Asp Gly Wing Asn Ser Wing 410 415 420 ttc ttc gcc tea gcg tgc gtg gca gtg ttt gca ctg atc gtg ggc ttc 1411 Phe Phe Wing Being Wing Cys Val Wing Val Phe Wing Leu He Val Gly Phe 425 430 435 ttt gta aag agg cca gcc cgc taagctaggt cgcatgatca gca 1455 Phe Val Lys Arg Pro Wing Arg 440 < 210 > 240 < 211 > 444 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 240 Met Met Leu Asn Glu Thr Thr Leu Wing Val Wing Leu Pro Being He Met 1 5 10 15 Wing Asp Phe Asp He Glu Wing Asn Thr Wing Gln Trp Leu Leu Thr Gly 20 25 •• 30 Phe Met Leu Thr Met Wing Val Val Leu Pro Wing Thr Gly Trp Met Leu 35 40 45 Glu Arg Phe Thr Thr Arg Ser Val Phe He Phe Wing Thr Val Val Phe 50 55 60 Leu He Gly Thr Val Thr Wing Ala Leu Ser Pro Thr Phe Wing He Met 65 70 75 80 Leu Ala Ala Arg Val Ala Gln Ala He Gly Thr Ala Val Met Met Pro 85 90 95 Leu Leu Met Thr Val Ala Met Thr Val Val Pro Pro Glu Arg Arg Gly 100 105 110 Wing Val Met Gly Leu He Wing Val Val Met Wing Val Gly Pro Wing Leu 115 120 125 Gly Pro Ser Val Wing Gly Phe Val Leu Ser Leu Ser Ser Trp His Wing 130 135 140 He Phe Trp Val Val Val Val Leu Val Phe Val Wing Ser Leu He Gly 145 150 155 160 Thr Leu Arg Leu Thr Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp 165 170 175 Val He Ser Phe Leu He Ser Wing Val Wing Phe Gly Gly Leu Val Tyr 180 185 190 Wing Leu Being Ser Gly He He Leu Glu Gly Asp Arg Being Wing Leu 195 200 205 Val Val Leu Ala Val Gly He He Ala Ala Val Val Phe Val Trp Arg 210 215 220 Gln He Ala Met Gly Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro 225 230 235 240 Leu Ala He Arg Glu Tyr Thr He Pro Leu Val Val Leu Leu Thr Leu 245 250 255 Phe Gly Ala Leu Leu Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln 260 265 270 Gly Ser Leu Met Val Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro 275 280 285 Gly Gly Leu Leu Glu Gly Val Leu Ser Pro Phe Val Gly Arg He Tyr 290 295 300 Asp Arg His Gly Pro Arg Gly Leu Val He Gly Gly Met Ser Leu Val 305 310 315 320 Val He Ser Leu Phe Ala Leu Ser Thr Val Asp Glu Phe Ala Asn Val 325 330. ' 335 Trp Phe He He Gly Val His He Val Phe Ser Zle Gly Leu Ala Leu 340. 345 350 Leu Phe Thr Pro Leu Met Thr Val Wing Leu Wing Ser Val Pro Asp Asn 355 360 365 Met Tyr Gly His Gly Ser Wing He Leu Asn Thr Leu Gln Gln Leu Wing 370 375 380 Gly Wing Wing Gly Thr Wing Val Met He Wing Val Tyr Ser Thr Val Ser 385 390 395 400 Asn Asn Wing Leu He Asp Gly Wing Thr Gln Gln Thr Wing Leu Wing Asp 405 410 415 Gly Wing Asn Being Wing Phe Phe Wing Being Wing Cys Val Wing Val Phe Wing 420 425 430 Leu He Val Gly Phe Phe Val Lys Arg Pro Wing Arg 435 440 i ..... »i". - - »J ^ - * J ...,. .. . . ,:. : cí? É - < 210 > 241 < 211 > 1093 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1093) < 223 > FRXA00565 < 400 > 241 tggagccttg tcttcctcca gcaatcccac aacggagcag gttgggatcc cgagaaatgt 60 tgtcatcatc ttggctgtat tagtttttac agcctttgtc atg atg ttg aat gag 115 Met Met Leu Asn Glu 1 5 act act ctg gca gtc gcg ttg ccg tcg atc atg gcg gac ttt gac att 163 Thr Thr Leu Ala Val Ala Leu Pro Be He Met Wing Asp Phe Asp He 10 15 20 gag gcg aat act gcg cag tgg ttg ctc act ggt ttt atg ttg acc atg 211 Glu Wing Asn Thr Wing Gln Trp Leu Leu Thr Gly Phe Met Leu Thr Met 25 30 35 gct gtg gtt ctt cca cct gct act ggt tgg atg ttg gaa cgt ttt acc act 259 Wing Val Val Leu Pro Wing Thr Gly Trp Met Leu Glu Arg Phe Thr Thr 40 45 50 cgt agt gtg ttt att ttc gcc acg gtg gtc ttc ctg atc ggt act gtg 307 Arg Ser Val Phe He Phe Ala Thr Val Val Phe Leu He Gly Thr Val 55 60 65 acg gct gcg ttg tct ect act ttt gcg att atg ctt gca gcc cgc gtc 355 Thr Ala Ala Leu Ser Pro Thr Phe Ala He Met Leu Ala Ala Arg Val 70 75 80 85 gct cag gcg att ggt acc gct gtg atc atg ccg ctg ctg atg act gtc 403 Ala Gln Ala He Gly Thr Ala Val He Met Pro Leu Leu Met Thr Val 90 95 100 gcg atg acc gtt gtt ect cca gag cgc cgt ggc gcc gtc atg ggt ttg 451 Wing Met Thr Val Val Pro Pro Glu Arg Arg Gly Wing Val Met Gly Leu 105 110 115 att gcg gtg atg gcc gtt ggt ect gct ctt gga ect agt gtg gct 499 He Ala Val Val Met Ala Val Gly Pro Ala Leu Gly Pro Ser Val Ala 120,125,130 GGT ttc gta ctc age ttg TCT TCG TGG CAC GCG att ttc TGG gtc atg 547 Gly Phe Val Leu Ser Leu Ser Ser Trp His Ala He Phe Trp Val Met 135 140 145 gtt ccg ttg gtg ttt gtg gca age ctg atc ggt acc ctg cgt ctg ctg 595 Val Pro Leu Val Phe Val Wing Ser Leu He Gly Thr Leu Arg Leu Thr 150 155 160 165 aac gtc agt gag ect aaa aag act ect ttg gat gtt att aka ttc ctg 643 Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp Val He Ser Phe Leu 170 175 180 att aka gca gtg gct ttc ggt ggc ctt gtg tac gcc ttg age tcg att 691 He Ser Ala Val Ala Phe Gly Gly Leu Val Tyr Ala Leu Ser Ser He 185 190. . 195 ggc atc att ttg gaa ggt gac aga age gct ttg gtc gtg ttg gct gtc 739 Gly He He Leu Glu Gly Asp Arg Ser Ala Leu Val Val Leu Ala Val 200 205 210 ggc atc att gcg ttg gtg gtg ttt gtg tgg cgc cag att gcc atg ggt 787 Gly He He Wing Leu Val Val Phe Val Trp Arg Gln He Wing Met Gly 215 220 225 aag cag gat aag gcg ctg ttg gat ctg cgt ccg ttg gcg att cgt gag 835 Lys Gln Asp Lys Ala Leu Leu Asp Leu Arg Pro Leu Ala He Arg Glu 230 235 240 245 tac acc att ccg ctg gtt gtg ctt ttg acg ctg ttc ggt gcg ctg ctc 883 Tyr Thr He Pro Leu Val Val Leu Leu Thr Leu Phe Gly Ala Leu Leu 250 255 260 ggt gtc atg aat here ctg ccg ctc tac ctg cag gga tcc ttg atg gtc 931 Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln Gly Ser Leu Met Val 265 270 275 acc gcc ttg gtc gcg ggt cta gtg ctg ttg cca ggt ggt ctt ttg gaa 979 Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro Gly Gly Leu Leu Glu 280 285 290 ggt gtg ctg tcg cca ttt gtg ggt cga att tat gat cgt cat ggt cca 1027 Gly Val Leu Ser Pro Phe Val Gly Arg He Tyr Asp Arg His Gly Pro Cgc gga 295 300 305 ctc ggc ggt atc gtg atg gtg gtt atc ctc tea ctg ttt aka 3075 Arg Gly Gly Gly Leu Val He Met Ser Leu Ser Leu Val Val Phe He 315 320 325 gca 310 ctg acc gtc gat 1093 aka Ala Leu Ser Thr Val Asp 330 < 210 > 242 < 211 > 331 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 242 Met Met Leu Asn Glu Thr Thr Leu Ala Val Ala Leu Pro Ser He Met 1 5 10. . 15 Wing Asp Phe Asp He Glu Wing Asn Thr Wing Gln Trp Leu Leu Thr Gly 20 25 30 Phe Met Leu Thr Met Wing Val Val Leu Pro Wing Thr Gly Trp Met Leu 35 40 45 Glu Arg Phe Thr Thr Arg Ser Val Phe He Phe Ala Thr Val Val Phe 50 55 60 Leu He Gly Thr Val Thr Ala Ala Leu Ser Pro Thr Phe Ala He Met 65 70 75 80 Leu Ala Ala Arg Val Ala Gln Ala He Gly Thr Ala Val He Met Pro 85 90 95 Leu Leu Met Wing Val Val Wing Met Val Val Pro Pro Glu Arg Arg Gly 100 105 110 Wing Val Met Gly Leu He Wing Val Val Met Wing Val Gly Pro Wing Leu 115 120 125 Gly Pro Ser Val Wing Gly Phe Val Leu Ser Leu Ser Ser Trp His Wing 130 135 140 He Phe Trp Val Met Val Pro Leu Val Phe Val Wing Ser Leu He Gly 145 150 155 160 Thr Leu Arg Leu Thr Asn Val Ser Glu Pro Lys Lys Thr Pro Leu Asp 165 170 175 Val He Be Phe Leu He Be Wing Val Wing Phe Gly Gly Leu Val Tyr 180 185 190 Wing Leu Being Be He Gly He He Leu Glu Gly Asp.'Arg Be Wing Leu 195 200 205 Val Val Leu Wing Val Gly He He Wing Leu Val Val Phe Val Trp Arg 210 215 220 Gln He Wing Met Gly Lys Gln Asp Lys Wing Leu Leu Asp Leu Arg Pro 225 230 235 240 Leu Ala He Arg Glu Tyr Thr He Pro Leu Val Val Leu Leu Thr Leu 245 250 255 Phe Gly Ala Leu Leu Gly Val Met Asn Thr Leu Pro Leu Tyr Leu Gln 260 265 270 Gly Ser Leu Met Val Thr Ala Leu Val Ala Gly Leu Val Leu Leu Pro 275 280 285 Gly Gly Leu Leu Glu Gly Val Leu Ser Pro Phe Val Gly Arg He Tyr 290 295 300 Asp Arg His Gly Pro Arg Gly Leu Val He Gly Gly. Met Ser Leu Val 305 310 315 • 320 Val He Ser Leu Phe Ala Leu Ser Thr Val Asp 325 330 < 210 > 243 < 211 > 380 < 212 > DNA < 213 > Corynebacterium glutamicum 5 < 220 > < 221 > CDS < 222 > (1) .. (357) < 223 > FRXA02878 < 400 > 243 tgc ctg tcc acc gtc gat gag ttc gcc acg tgt tgg tea tea tcc gcg 48 Cys Leu Ser Thr Val Asp Glu Phe Wing Thr Cys Trp Ser Ser Phe Wing 1 5 10 15 gac aca tcg tgg ttc tea tcg gcc ctt gcg ctg ctg ttc acc cca ctg 96 Asp Thr Be Trp Phe Ser Be Ala Leu Ala Leu Leu Phe Thr Pro Leu 10 20 25 30 atg aca gtc gcg ctc gca tcc gtc ccc gac aac atg tac ggc cac ggc 144 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly 35 40 45 tcc gcg atc ctc aac c ctc aac cctc ca gc gc gc gcc gcc gcc acc 192 Ser Ala He Leu Asn Thr Leu Gln Gln Leu Ala Gly Ala Wing Gly Thr 50 55 60 gcg gtc atg att gcg gtt tat tcc acc gtc age aac aac gcg ctt atc 240 Wing Val Met He Wing Val Tyr Ser Thr Val Ser Asn Asn Wing Leu He 15 65 70 75 80 gac ggc gca acc caa caa acc gcc ctc gcc gac ggc gcc aac tct gca 288 Asp Gly Wing Thr Gln Gln Thr Wing Wing Leu Wing Asp Gly Wing Asn Being Wing 85 90 '95 ttc ttc gc gc gcg tgc gtg gca gtg gtc tt gca gtg gtc gtc ttc 336 Phe Phe Wing Being Wing Cys Val Wing Val Phe Wing Leu He Val Gly Phe 100 105 110 ttt gta aag agg cca gcc cgc taagctaggt cgcatgatca gca 380 Phe Val Lys Arg Pro Ala Arg 20 H5 < 210 > 244 < 211 > 119 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 244 Cys Leu Ser Thr Val Asp Glu Phe Wing Thr Cys Trp Ser Ser Phe Wing 1 5 10 15 25 Asp Thr Ser Trp Phe Ser Be Ala Leu Ala Leu Leu Phe Thr Pro Leu 20 25 30 Met Thr Val Ala Leu Ala Ser Val Pro Asp Asn Met Tyr Gly His Gly 35 40 45 Be Ala He Leu Asn Thr Leu Gln Gln Leu Ala Gly Ala Ala Gly Thr 50 55 60 Ala Val Met He Ala Val Tyr Ser Thr Val Ser Asn Asn Ala Leu He 65 70 75 80 Asp Gly Wing Thr Gln Gln Thr Wing Leu Wing Asp Gly Wing Asn Being Wing 85 90 95 Phe Phe Wing Being Wing Cys Val Wing Val Phe Wing Leu He Val Gly Phe 100 105 110 Phe Val Lys Arg Pro Wing Arg 115 < 210 > 245 < 211 > 1533 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1510) < 223 > RXA00648 < 400 > 245 gtttgccagc tattgcatct tgcacaaaat gtgtaccata cacataatgt catcgagtcc 60 tcccgaatca gccacaccac agatcaaata cggcctgctg gtg gtc act ctc gcc 115 Val, Val Thr Leu Wing 1 '5 tea gct ggt atc act gtt tcc cta gcg cag acc ctg gtt att ccg atc 163 Ser Wing Gly He Thr Val Ser Leu Wing Gln Thr Leu Val He Pro He 10 15 20 att ggt cgg ttg ccc gag atc tcc aac acc accct gct gct aat gcc tct 211 He Gly Arg Leu Pro Glu He Phe Asn Thr Thr Wing Wing Asn Wing Being 25 30 35 tgg atc att act gtg acg ctg ttg gtg ggc gca gtg gcg act ect gtg 259 Trp He He Thr Val Thr Leu Leu Val Gly Wing Val Wing Thr Pro Val 40 45 50 atg ggc agg ctt gca gat atg tac ggc aag aaaaag atg atg ctc atc 307 Met Gly Arg Leu Wing Asp Met Tyr Gly Lys Lys Met Met Met Leu He 55 60 _ 65 tea ctt gtc ccg ttc att ctt gga tea gtg atc tgc gct gtg tcg gtg 355 Ser Leu Val Pro Phe He Leu Gly Ser Val He Cys Wing Val Ser Val 70 75 80 85 gat ttg att ccg atg atc atc ggc cgt ggt ttt cag ggg ctt ggc tct 403 Asp Leu He Pro Met He He Gly Arg Gly Phe Gln Gly Leu Gly Ser 90 f 95 '. 100 ggc ctg att ect ctt ggc att tct ctc atg cat gat ttg tcg ccc cgg 451 Gly Leu He Pro Leu Gly He Ser Leu Met His Asp Leu Leu Pro Arg 105 110 115 gag aaa gca ggg tct gcc att gct ttg atg agt tct tcc atg ggc att 499 Glu Lys Wing Gly Wing Wing Wing Wing Leu Met Being Ser Gly Gly 120 120 130 ggc ggt gct gct ctc ccg ctg gct gct gct att gcc cag ttt gcg 547 Gly Gly Wing Leu Gly Leu Pro Wing Ala Leu Wing Wing Gln Phe Wing 135 140 145 tcc tgg cgg gtg ctg ttc tgg ttc acc gct ctg gta gcg ctt aca gtt 595 Ser Trp Arg Val Leu Phe Trp Phe Thr Ala Leu Val Ala Leu Thr Val 150 155 160 165 10 ggc gcg gtc att tgg aag gcg att ect gct aga ccc agg atc gtg agg 643 Gly Ala Val He Trp Lys Ala He Pro Ala Arg Pro Arg He Val Arg 170 175 180 agt ggc ggc ttt gat tat ttc ggt gct ctc ggc ctt gca atg gga ctt 691 Be Gly Gly Phe Asp Tyr Phe Gly Ala Leu Gly Leu Ala Met Gly Leu 185 190 - 195 atc gca ttg ttg ctc gcg gtg tcc aag gga tea gaa tgg ggc tgg aga 739 He Ala Leu Leu Leu Ala Val Ser Lys Gly Ser Glu Trp Gly Trp Arg 200 205 210 15 agt gcc ctg acc att ggg tta ttc gtg gca gcg ctg gtg att ttg gtg 787 Be Ala Leu Thr He Gly Leu Phe Val Ala Ala Leu Val He Leu Val 215 220 225, • ggt tgg ggc tgg ttc gaa acc cgc cag aaa tcc ect tt-g att gat ctg 835 Gly Trp Gly Trp Phe Glu Thr Arg Gln Lys Ser Pro Leu He Asp Leu 230 235 240 245 cgc acc act att cgg gcg acc gtg ttg atg aca aat att gcg tcc atc 883 Arg Thr Thr He Arg Wing Thr Val Leu Met Thr Asn He Wing Be He 250 255 260 0 ctc atc ggt ttc acc atg tat gga atg aat ctg atc ctg ect cag gtc 931 Leu He Gly Phe Thr Met Tyr Gly Met Asn Leu He Leu Pro Gln Val 265 270 275 atg cag ctg ect gta att ctg ggc tac ggt cta ggc cag age atg ctt 979 Met Gln Leu Pro Val He Leu Gly Tyr Gly Leu Gly Gln Ser Met Leu 280 285. 290 cag atg ggc atc tgg ctg atc ccg atg ggt cta ggc atg atg ttg att 1027 Gln Met Gly He Trp Leu He Pro Met Gly Leu Gly Met Met Leu He 295 300 305 5 tcg aat gca ggt gca gcc att age gct gct cat ggt ect cgt gtg acg 1075 Be Asn Ala Gly Ala Ala Be Ser Ala Ala His Gly Pro Arg Val Thr 310 315 320 325 ctg aca att gcg ggt gtt gtg atc gca gtc ggt tat gca ctt act gcc 1123 Leu Thr He Wing Gly Val Val He Wing Val Gly Tyr Ala Leu Thr Wing 330 335 340 here gtg ttg ttc act atc ggc aac cgc aca ccg gga gga gat gca gac 1171 Thr Val Leu Phe Thr He Gly Asn Arg Thr Pro Gly Gly Asp Wing Asp 345 350 355 aac gca ctt att ttg acc acc ctg gtg ctg ttc tea gtg tgt agt ctc 1219 Asn Ala Leu He Leu Thr Thr Leu Val Leu Phe Ser Val Cys Ser Leu 360 365 370 gtg gtc ggt atc ggc att ggc ctg gca ttt ggt tcc atg ect gcc ttg 1267 Val Val Gly He Gly He Gly Leu Wing Phe Gly Ser 'Met Pro Wing Leu 375 380 385 tie atg ggt gcc gta cca gcc acg gag aaa gcc gca gcg aat ggt ttc 1315 He Met Gly Wing Val Pro Wing Thr Glu Lys Wing Wing Wing Asn Gly Phe 390 395 400 405 aac tct ctt atg cgt tea ctg ggc acc acc ggc tea tea gct gtc atc 1363 Asn Ser Leu Met Arg Ser Leu Gly Thr Thr Gly Ser Ser Wing Val He 410 415 420 ggt gca gtg ttg gcc gga atg atg agt ggc gga gta ccc acc tta ggg 1411 Gly Wing Val Leu Wing Gly Met Met Ser Gly Gly Val Pro Thr Leu Gly 425 430 435 gga ttc atg acc act ctg atc atc gga tgc tgc gcc gcg ctt gtg gct 1459 Gly Phe Met Thr Thr Leu He He Gly Cys Cys Ala Wing Leu Val Wing 440 445 450 gcg gtc atc tcc tat ttc atc ccc acc acc acct gtg gtg gaa gca gca 1507 Ala Val He Ser Tyr Phe He Pro Thr Thr Thr Thr Val Val Glu Ala 455 460 465- 'aaa taatcccggc agegactega cca 1533 Lys 470 < 210 > 246 < 211 > 470 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 246 Val Val Thr Leu Ala Be Wing Gly He Thr Val Ser Leu Wing Gln Thr 1 5 10 15 Leu Val He Pro He He Gly Arg Leu Pro Glu He -Phe Asn Thr Thr 20 25 30 i. *. * -.

Wing Wing Asn Wing Being Trp He He Thr Val Thr Leu Leu Val Gly Wing 35 40 45 Val Wing Thr Pro Val Met Gly Arg Leu Wing Asp Met Tyr Gly Lys Lys 50 55 60 Lys Met Met Leu He Ser Leu Val Pro Phe He Leu Gly Ser Val He 65 70 75 80 Cys Ala Val Ser Val Asp Leu He Pro Met He He Gly Arg Gly Phe 85 90 95 Gln Gly Leu Gly Ser Gly Leu He Pro Leu Gly He Ser Leu Met His 100 105 110 Asp Leu Leu Pro Arg Glu Lys Wing Gly Be Wing He Wing Leu Met Ser 115 120 125 Ser Ser Met Gly He Gly Gly Ala Leu Gly Leu Pro Leu Ala Ala Ala 130 135 140 He Ala Gln Phe Ala Ser Trp Arg Val Leu Phe Trp Phe Thr Ala Leu 145 150 155 160 Val Ala Leu Thr Val Gly Ala Val He Trp Lys Ala He Pro Ala Arg 165 170 175 Pro Arg He Val Arg Ser Gly Gly Phe Asp Tyr Phe Gly Wing Leu Gly 180 185 190 Leu Wing Met Gly Leu He Wing Leu Leu Leu Wing Val Ser Lys Gly Ser 195 200 205 Glu Trp Gly Trp Arg Ser Wing Leu Thr He Gly Leu Phe Val Ala Ala 210 215 220 Leu Val He Leu Val Gly Trp Gly Trp Phe Glu Thr Arg Gln Lys Ser 225 230 235 240 Pro Leu He Asp Leu Arg Thr Thr He Arg Wing Thr Val Leu Met Thr 245 250 255 Asn He Wing Being He Leu He Gly Phe Thr Met Tyr -Gly Met Asn Leu 260 265 270 He Leu Pro Gln Val Met Gln Leu Pro Val He Leu Gly Tyr Gly Leu 275 280 285 Gly Gln Being Met Leu Gln Met Gly He Trp Leu He Pro Met Gly Leu 290 295 300 Gly Met Met Leu He Ser Asn Ala Gly Ala Wing He Ser Ala Ala His 305 310 315 320 Gly Pro Arg Val Thr Leu Thr He Wing Gly Val Val He Wing Val Gly 325 330 335 .. .. " to, * ........... . . _ "" ...

Tyr Ala Leu Thr Ala Thr Val Leu Phe Thr He Gly Asn Arg Thr Pro 340 345 ''. 350 Gly Gly Asp Wing Asp Asn Wing Leu He Leu Thr Thu Leu Val Leu Phe 355 360 365 Ser Val Cys Ser Leu Val Val Gly He Gly He Gly Leu Wing Phe Gly 370 375 380 Ser Met Pro Ala Leu He Met Gly Ala Val Pro Wing Thr Glu Lys Wing 385 390 395 400 Wing Wing Asn Gly Phe Asn Being Leu Met Arg Being Leu Gly Thr Thr Gly 405 410 415 Sering Wing Val He Gly Wing Val Leu Wing Gly Met Met Ser Gly Gly 420 425 430 Val Pro Thr Leu Gly Gly Phe Met Thr Thr Leu He He Gly Cys Cys 435 440 '445 Ala Ala Leu Val Ala Ala Val He Ser Tyr Phe He Pro Thr Thr Thr 450 455 460 Thr Val Val Glu Ala Lys 465 470 < 210 > 247 < 211 > 1770 < 212 > DNA < 213 >; Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1747) < 223 > RXN01320 < 400 > 247 gtgaatggca cgacatgcca caaggcacgc aagctgattt ccaagcctgc tgtcgcaaag 60 caattaaaaa tacttttctt cttagaggtg gattttcaga atg aca tea cag gtc 115 Met Thr Ser Gln Val 1 5 aag ccg gac gac cgt ccg gta aca aca att tea aaa agt ggt gca 163 Lys Pro Asp Asp Glu Arg Pro Val Thr Thr He Ser Lys Ser Gly Wing 10 15 20 ect tcg gcc cac acc gca tea gca gca gca gca gca gca gca act gaa gaa 211 Pro Ser Wing His Thr Ser Wing Pro Tyr Gly Ala Wing Wing Thr Glu Glu 25 30 35 gct gtc gag gaa aaa acc aaa ggt cgc gtt gga ttt atc atc gca gcc 259 Wing Val Glu Glu Lys Thr Lys Gly Arg Val Gly Phe He He Wing Wing 40 45 50 - '*' * ctc atg ttg gcg atg ctt ctt age tcc tg ggt cag acc att ttc ggt 307 Leu Met Leu Wing Met Leu Leu Being Ser Leu Gly Gln Thr He Phe Gly 55 60 65 tct gcc ctg cca acg att gtt ggt gag ctt ggc ggc gtt aac cac atg 355 Be Ala Leu Pro Thr He Val Gly Glu Leu Gly Val Asn His Met 70 75 80 85 acc tgg gtg att acc gcc ttc ctc ttg ggc cag acc att tea ttg ect 403 Thr Trp Val He Thr Wing Phe Leu Leu Gly Gln Thr He Ser Leu Pro 90 95 100 att ttc ggc aag ttg ggt gac cag ttt ggt cgc aaa tac ctc ttc atg 451 He Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg Lys Tyr Leu Phe Met 105 110 115 ttt gcc atc gca ctg ttc gtg gtg ggt tcc atc atc ggt gct ttg gct 499 phe wing he wing wing phe val val gly be he he gly gly wing leu wing 120 125 130 cag aac atg acc acc ttg att gtg gct cgt gca ctg cag ggt atc gcc 547 Gln Asn Met Thr Thr Leu He Val Wing Arg Wing Leu Gln Gly He Wing 135 140 145 ggt ggt ggc ttg atg att ctt tct cag gca att acc gct gat gtc acc 595 Gly Gly Gly Leu Met He Leu Ser Gln Ala He Thr Ala As p Val Thr 150 155 160 165 acc gcc cgt gag cgt gca aag tac atg ggc atc at'g ggt tcc gtt ttc 643 Thr Wing Arg Glu Arg Wing Lys Tyr Met Gly He Met Gly Ser Val Phe 170 175 180 gga ctg tcc tcc atc ctt ggc cca ttg ctt ggt ggc tgg tcc act gac 691 Gly Leu Ser Ser He Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Asp 185 190 195 ggt cca ggc tgg cgt tgg ggt ctg tgg ttg aac gtt cca atc ggc atc 739 Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn Val Pro He Gly He 200 205 210 atc gca ctg gtt gct atc gct gtg ctg ctg aaa ctt cca cct gct cgt gaa 787 He Ala Leu Val Ala He Ala Val Leu Leu Lys Leu Pro Ala Arg Glu 215 220 225 cgt ggc aag gtc tcc gtt gac tgg tg gga age atc ttc atg gct atc 835 Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser He Phe Met Ala He 230 235 240 245 gcc acc acc gca ttt gtc ctc gca gtg acc tgg ggt ggc aat gaa tat 883 Wing Thr Thr Wing Phe Val Leu Wing Val Thr Trp Gly Gly Asn Glu Tyr 250 255 260 gag tgg gca tea cca atg atc atc ggt ttg ttc atc acg aca ttg gtc 931 Glu Trp Ala Ser Pro Met He He Gly Leu Phe He Thr Thr Leu Val 265 270 275 gct gcg ata gtg ttc gtt ttc gtc gaa aag cgt gct gtt gac cca ctg 979 Wing Wing Val Val Val Plu Phe Val W Val Val Val Val Llu Arg Ala Val Asp Pro Leu 280 285 290 gtc ccc atg ggc ctt tcc tcc aac ccc aac tcc ctc ccc acc gcc gcc 1027 Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val Leu Thr Wing Val 295 300 305 gcc ggt atc ggc gta ggc ctg ttt atg ggc acc atc gcg tac atg 1075 5 Wing Gly He Gly Val Gly Leu Phe Met Met Gly ffhr He Ala Tyr Met • 310 315 320 '325 ect acc tac ctg cag atg gtt cat ggt ctg aac cca acg caa gct ggt 1123 Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro Thr Gln Wing Gly 330 335 340 ctg atg ctg atc cca atg atg atc ggc ctg att ggt aca tcc act gtg 1171 Leu Met Leu He Pro Met Met He Gly Leu He Gly Thr Ser Thr Val 345 350 355 gtg ggc aac atc gtg tcc aag act ggc aag tac aag tgg tac cc tcc 1219 10 Val Gly Asn He Val Ser Lys Thr Gly Lys Tyr Lys Trp Tyr Pro Phe 360 365 370 atc ggc atg ctc atc atg gtc ctt gcc cta gta ctg cta tcg acg ctg 1267 He Gly Met Leu He Met Val Leu Ala Leu Val Leu Leu Ser Thr Leu 375 380 385 aca ect tcg gca age ttg gct ctc a tt gga ctg tac ttc ttc gtc gtc ttc 1315 Thr Pro Ser Ala Be Leu Ala Leu He Gly Leu Tyr Phe Phe Val Phe 390 395 400 405 gga ttc ggc ctg ggc tgt gca atg cag att ttg gtt ctc atc gtg cag 1363 15 Gly Phe Gly Leu Gly Cys Wing Met Gln He Leu Val Leu He Val Gln 410 415 420 aac tcc ct atc acc atg gtt ggc acc gcg acc gct tcc aac aac 1411 Asn Ser Phe Pro He Thr Met Val Gly Thr Wing Thr Gly Ser Asn Asn 425 430 435 ttc ttc cgc caa atc ggt gga gca gta ggt tcc gca ctg atc ggt ggc 1459 Phe Phe Arg Gln He Gly Gly Wing Val Gly Ser Ala Leu He Gly Gly 440 445 450 ctg ttt atc tcc aac ctg tcc gac cga ttc acc gaa aac gtc ccc gca 1507 20 Leu Phe He Ser Asn Leu Ser Asp Arg Phe Thr Glu Asn Val Pro Wing 455 460 465 gca gtg gct tcc atg ggt gaa gaa ggc gca caa tac gcc tea gca atg 1555 Ala Val Ala Ser Met Gly Glu Glu Gly Ala Gln Tyr Ala Wing Wing Met 470 475 480 485 tcc gat ttc tcc tc ggt gca tcc aac cct act cca cac ctt gtt gaa tea 1603 Ser Asp Phe Ser Gly Ala Ser Asn Leu Thr Pro His Leu Val Glu Ser 490 495 50 0 ctt cca caca gca ctc cgt gaa gca att caa ctt tct tac aac gac gcc 1651 25 Leu Pro Gln Ala Leu Arg Glu Ala He Gln Leu Ser Tyr Asn Asp Ala MMWHHMYH-ia ii * T- »* - • - '- 505 510 515 ctg aca cca atc ttc ttg gcg ctc acc ccg atc gca gta gc gcc gcg 1699 Leu Thr Pro He Phe Leu Ala Leu Thr Pro He Wing Val Val Wing Wing 520 525 530 atc ctc ctc ttt ttc atc cgt gaa gat cac ctc aag gaa acg cac gaa 1747 He Leu Leu Phe Phe He Arg Glu Asp His Leu Lys Glu Thr His Glu 535 540 545 taatgacaca cgaaacttcc gtc 1770 < 210 > 248 < 211 > 549 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 248 Met Thr Ser Gln Val Lys Pro Asp Asp Glu Arg Pro Val Thr Thr He 1 5 10 15 Ser Lys Ser Gly Pro Wing Ser Wing His Thr Ser Wing Pro Tyr Gly Wing 20 25 30 Wing Wing Thr Glu Wing Wing Val Glu Glu Lys Thr Ly's Gly Arg Val Gly 35 40 45 Phe He He Ala Ala Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly 50 55 60 Gln Thr He Phe Gly Be Ala Leu Pro Thr He Val Gly Glu Leu Gly 65 70 75 80 Gly Val Asn His Met Thr Trp Val He Thr Wing Phe Leu Leu Gly Gln 85 90 95 Thr He Ser Leu Pro He Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg 100 105 110 Lys Tyr Leu Phe Met Phe Ala He Ala Leu Phe Val Val Gly Be He 115 120. 125 He Gly Ala Leu Ala Gln Asn Met Thr Thr Leu He Val Ala Arg Ala 130 135 140 Leu Gln Gly He Wing Gly Gly Gly Leu Met He Leu Ser Gln Wing He 145 150 155 160 Thr Wing Asp Val Thr Thr Wing Arg Glu Arg Wing Lys Tyr Met Gly He 165 170 175 Met Gly Ser Val Phe Gly Leu Ser Be He Leu Gly Pro Leu Leu Gly 180 185 190 Gly Trp Phe Thr Asp Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn 195 200. 205 - »- * - - * -» - "- - - - -" - - * Val Pro He Gly He He Wing Leu Val Wing He Wing Val Leu Leu Lys 210 215 220 Leu Pro Wing Arg Glu Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser 225 230 235 240 He Phe Met Wing He Wing Thr Thr Wing Phe Val Leu Wing Val Thr Trp 245 250 255 Gly Gly Asn Glu Tyr Glu Trp Wing Pro Met Met He He Gly Leu Phe 260 265 270 He Thr Thr Leu Val Wing Wing He Val Phe Val Phe Val Glu Lys Arg 275 280 285 Ala Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe 290 295 300 Val Leu Thr Ala Val Ala Gly He Gly Val Gly Leu Phe Met Met Gly 305 310 315 320 Thr He Ala Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn 325 330 335 Pro Thr Gln Wing Gly Leu Met Leu He Pro Met Met He Gly Leu He 340 345 350 Gly Thr Ser Thr Val Val Gly Asn He Val Ser Lys Thr Gly Lys Tyr 355 360 365 Lys Trp Tyr Pro Phe He Gly Met Leu He Met Val Leu Ala Leu Val 370 375 380 Leu Leu Ser Thr Leu Thr Pro Ser Ala Ser Leu Ala Leu He Gly Leu 385 390 395 400 Tyr Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Ala Met Gln He Leu 405 410 415 Val Leu He Val Gln Asn Ser Phe Pro He Thr Met Val Gly Thr Wing 420 425 430 Thr Gly Being Asn Asn Phe Phe Arg Gln He Gly Gly Wing Val Gly Ser 435 440 445 Wing Leu He Gly Gly Leu Phe He Being Asn Leu Being Asp Arg Phe Thr 450 455 460 Glu Asn Val Pro Wing Wing Val Wing Being Met Gly Glu Glu Gly Wing Gln 465 470 475 480 Tyr Ala Being Wing Met Being Asp Phe Ser Gly Wing Being Asn Leu Thr Pro 485 490 495 His Leu Val Glu Ser Leu Pro Gln Ala Leu Arg Glu Ala He Gln Leu 500 505 510 Ser Tyr Asn Asp Ala Leu Thr Pro He Phe Leu Ala Leu Thr Pro He 515 520 525 Ala Val Val Ala Ala He Leu Leu Phe Phe He Arg Glu Asp His Leu 530 535 540 Lys Glu Thr His Glu 545 < 210 > 249 < 211 > 841 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (841) < 223 > FRXA01314 10 < 400 > 249 gtgaatggca cgacatgcca caaggcacgc aagctgattt ccaagcctgc tgtcgcaaag 60 caattaaaaa tacttttctt cttagaggtg gattttcaga atg aca tea cag gtc 115 Met- Thr Ser Gln Val 1 5 aag ccg gac gac gag cgt ccg gta aca aca att tea aaa agt ggt gca 163 Lys Pro Asp Asp Glu Arg Pro Val Thr Thr He Ser Lys Ser Gly Wing 10 15 20 15 ect tcg gcc cac acc gca gca ca gca gca gca gca gca gca act gaa gaa 211 Pro Ser Ala His Thr Ser Ala Pro Tyr Gly Ala Ala Ala Thr Glu Glu 25 30 35 gct gtc gag gaa aaa acc aaa ggt cgc gtt gga ttt atc atc gc gcc 259 Wing Val Glu Glu Lys Thr Lys Gly Arg Val Gly Phe He He Wing Wing 40 45 50 ctc atg ttg gcg atg ctt ctt age tcc ttg ggt cag acc att ttc ggt 307 Leu Met Leu Wing Met Leu Leu Be Ser Leu Gly Gln Thr He Phe Gly 55 60 65 20 tct gcc ctg cca acg att gtt ggt gag ctt ggc ggc gtt aac cac atg 355 Ser Ala Leu Pro Thr He Val Gly Glu Leu Gly Gly Val Asn His Met 70 75 80 85 acc tgg gtg att acc gcc ttc ctc ttg ggc cag acc tt tt tct ect 403 Thr Trp Val He Thr Wing Phe Leu Leu Gly Gln Thr He Ser Leu Pro 90 95 100 att ttc ggc aag ttg ggt gac cag ttt ggt cgc aaa tac ctc ctc atg 451 He Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg Lys Tyr Leu Phe Met 105 110 115 25 ttt gcc atc gca ctg gtg ttc gtg gtc gtc atc atc ggt gct gtg gct tgc 499 Phe Wing Wing Leu Phe Val Val Gly Ser He He Gly Wing Leu Wing 120 125 130 cag aac atg acc ttg att gtg gct cgt gca ctg cag ggt atc gcc 547 Gln Asn Met Thr Thr Leu He Val Wing Arg Wing Leu Gln Gly He Wing 135 140 45 g g 45 140 140 140 140 140 140 140 acc acc acc acc acc acc acc acc 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 59 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 135 As 135 59 150 155 160 - 165 acc gcc cgt gag cgt gca aag tac atg ggc atc atg ggt tcc gtt ttc 643 Thr Wing Arg Glu Arg Wing Lys Tyr Met Gly He Met Gly Ser Val Phe 170 175 180 gga ctg tcc tcc tc tc tc tc tc tc tc tc tc tc tcc act gac 691 Gly Leu Ser Be He Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Asp 185 190 195 ggt cca ggc tgg cgt tgg ggt ctg tgg ttg aac gtt cca atc ggc atc 739 Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn Val Pro He Gly He 200 205 210 atc gca ctg gtt gct atc gct gtg ctg ctg aaa ctt cca gct cgt gaa 787 He Ala Ala Leu Ala He Ala Ala Leu Leu Lys Leu Pro Ala Arg Glu 215 220 225 cgt ggc aag gtc tcc gtt gac tgg ttg gga age atc ttc atg gct atc 835 Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser He Phe Met Wing He 230 235 240 • 245 gcc acc 841 Thr wing < 210 > 250 < 211 > 247 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 250 Met Thr Ser Gln Val Lys Pro Asp Asp Glu Arg Pro Val Thr Thr He 1 5 10 15 Ser Lys Ser Gly Wing Pro Ser Wing His Thr Ser Wing Pro Tyr Gly Wing 20 25 30 Wing Wing Thr Glu Glu Wing Val Glu Glu Lys Thr Iiys Gly Arg Val Gly 35 40 '45 Phe He He Ala Ala Leu Met Leu Ala Met Leu Leu Ser Ser Leu Gly 50 55 60 Gln Thr He Phe Gly Be Ala Leu Pro Thr He Val Gly Glu Leu Gly 65 70 75 80 Gly Val Asn His Met Thr Trp Val He Thr Wing Phe Leu Leu Gly Gln 85 90 95 Thr He Ser Leu Pro He Phe Gly Lys Leu Gly Asp Gln Phe Gly Arg 100 105 110 Lys Tyr Leu Phe Met Phe Ala He Ala Leu Phe Val Val Gly Ser He 115 120 125 He Gly Ala Leu Ala Gln Asn Met Thr Thr Leu He Val Ala Arg Ala 130 135 140 Leu Gln Gly He Wing Gly Gly Gly Leu Met He Leu Ser Gln Wing He 145 150 155 160 Thr Wing Asp Val Thr Thr Wing Arg Glu Arg Wing Lys Tyr Met Gly He 165 170 175 Met Gly Ser Val Phe Gly Leu Ser Ser He Leu Gly Pro Leu Leu Gly 180 185 190 10 Gly Trp Phe Thr Asp Gly Pro Gly Trp Arg Trp Gly Leu Trp Leu Asn 195 200 205 Val Pro He Gly He He Wing Wing Leu Val Wing He Wing Val Leu Leu Lys 210 215 220 Leu Pro Wing Arg Glu Arg Gly Lys Val Ser Val Asp Trp Leu Gly Ser 225 230 235 240 He Phe Met Wing He Wing Thr 245 15 < 210 > 251 < 211 > 803 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (780) < 223 > FRXA01320 20 < 400 > 251 gtt gac cca ctg gtc ccc atg ggc ctt tcc tcg aac cgc aac ttc gtg 48 Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val 1 5 10 15 ctc acc gcc gc gc gc gcc atc gc gc gc ctg atg atg ggc acc 96 Leu Thr Ala Val Ala Gly He Gly Val Gly Leu Phe Met Met Gly Thr 20 25 • 30 atc gcg tac atg ect acc tac ctg cag atg gtt cat ggt ctg aac cca 144 He Ala Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro 35 40 45 25 acg caa gct ggt ctg atg ctg atc cca atg atg atc ggc ctg att ggt 192 Thr Gln Wing Gly Leu Met Leu He Pro Met Met He Gly Leu He Gly 50 55 60 aca tcc act gtg gtg ggc aac atc gtg tcc aag act ggc aag tac aag 240 Thr Ser Thr Val Val Gly Asn He Val Ser Lys Thr Gly Lys Tyr Lys 65 70 75 80 tgg tac cca ttc atc ggc atg ctc atc atg gtc ctt gcc cta gta ctg 288 Trp Tyr Pro Phe He Gly Met Leu He Met Val Leu Ala Leu Val Leu 85 90 95 cta tcg acg ctg aca ect tcg gca age ttg gct ctc att gga ctg tac 336 Leu Be Thr Leu Thr Pro Be Ala Be Leu Ala Leu He Gly Leu Tyr 100 105 110 ttc ttc gtc ttc gtc gtc gtc ggc tgt gg atg cag att gt ttg gt 384 Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Wing Met Gln He Leu Val 115 120 125 ctc atc gtg cag aac tcc ttc cca atc acc atg gtt ggc acc gcg acc 432 Leu He Val Gln Asn Ser Phe Pro He Thr Met Val Gly Thr Ala Thr 130 135 140 ggt tcc aac aac ttc ttc cgc caa atc ggt gga gca gta ggt tcc gca 480 Gly Ser Asn Asn Phe Phe Arg Gln He Gly Gly Wing Val Gly Be Wing 145 150 155 160 ctg atc ggt ggc ctg ttt atc tcc aac ctg tcc gac cga ttc acc gaa 528 Leu He Gly Gly Leu Phe He Being Asn Leu Being Asp Arg Phe Thr Glu 165 170 175 aac gtc ccc gca gca gtg gct tcc atg ggt gaa gag ggc gca caa tac 576 Asn Val Pro Ala Ala Val Ala Ser Met Gly Glu Glu Gly Wing Gln Tyr 180 185 190 gcc tea gca atg tcc gat ttc tcc gcc gcc gcc tcc aac cct act cca cac 624 Wing Being Wing Met Being Asp Phe Ser Gly Wing Being Asn -Leu Thr Pro His 195 200 205 ctt gtt gaa tea ctt cca caca gca ctc cgt gaa gca att caa ctt tct 672 Leu Val Glu Ser Leu Pro Gln Ala Leu Arg Glu Ala He Gln Leu Ser 210 215 220 tac aac gac gcc ctg aca cca atc ttc ttg gcg ctc acc ccg atc gca 720 Tyr Asn Asp Ala Leu Thr Pro He Phe Leu Ala Leu Thr Pro He Wing 225 230 235 240 gta gtc gcc gcg atc ctc ctc ttt ttc atc cgt gaa gat cac ctc aag 768 Val Val Ala Ala He Leu Leu Phe He Arg Glu Asp His Leu Lys 245 250 255 gaa acg cac gaa tagagacaca cgaaacttcc gtc 803 Glu Thr His Glu 260 <; 210 > 252 < 211 > 260 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 252 Val Asp Pro Leu Val Pro Met Gly Leu Phe Ser Asn Arg Asn Phe Val 1 5 10 15 Leu Thr Ala Val Ala Gly He Gly Val Gly Leu Phe Met Met Gly Thr 20 25 30 He Wing Tyr Met Pro Thr Tyr Leu Gln Met Val His Gly Leu Asn Pro 35 40 45 Thr Gln Wing Gly Leu Met Leu He Pro Met Met He Gly Leu He Gly 50 55 60 Thr Ser Thr Val Val Gly Asn He Val Ser Lys Thr Gly Lys Tyr Lys 65 70 75 80 Trp Tyr Pro Phe He Gly Met Leu He Met Val Leu Ala Leu Val Leu 85 90 95 Leu Be Thr Leu Thr Pro Be Ala Be Leu Ala Leu He Gly Leu Tyr 100 105 110 Phe Phe Val Phe Gly Phe Gly Leu Gly Cys Ala Met Gln He Leu Val 115 120 125 Leu He Val Gln Asn Ser Phe Pro He Thr Met Val Gly Thr Ala Thr 130 135 14T Gly Ser Asn Asn Phe Phe Arg Gln He Gly Gly Wing Val Gly Ser Wing 145 150 155 160 Leu He Gly Gly Leu Phe He Being Asn Leu Being Asp Arg Phe Thr Glu 165 170 175 Asn Val Pro Wing Wing Val Wing Wing Met Gly Glu Glu Gly Wing Gln Tyr 180 185, 190 Wing Being Wing Met Being Asp Phe Ser Gly Wing Being Asn Leu Thr Pro His 195 200 205 Leu Val Glu Ser Leu Pro Gln Wing Leu Arg Glu Ala He Gln Leu Ser 210 215 220 Tyr Asn Asp Ala Leu Thr Pro He Phe Leu Ala Leu Thr Pro He Ala 225 230 235 - 240 Val Val Ala Ala He Leu Leu Phe Phe He Arg Glu Asp His Leu Lys 245 250 255 Glu Thr His Glu • '. 260 - < 210 > 253 < 211 > 1755 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS 5 < 222 > (101) .. (1732) < 223 > RXN02926 < 400 > 253 ccctgacacc aatcttcttg gcgctcaccc cgatcgcagt agtcgccgcg atcctcctct 60 ttttcatccg tgaagatcac ctcaaggaaa cgcacgaata atg aca cac gaa act 115 Met Thr His Glu Thr 1 5 tcc gtc ccc gga ect gcc gac gcg cag gtc gca gga gat acg aag ctg 163 Ser Val Pro Gly Pro Ala Asp Ala Gln Val Wing Gly Asp Thr Lys Leu 10 10 15 20 cgc aaa ggc cgc gcg aag aag aa g aa aa aa ect tea at tc acg ect 211 Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro Ser Ser Met Thr Pro • 25 30 35 gaa caa caa aag aaa gtc tgg tgg gtc ctc age gcg ctg atg gtc gcc 259 Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser Ala Leu Met Val Ala 40 45 50 atg atg atg gcc aka ctt gac cag atg att ttc ggc aca gcc ctg cca 307 Met Met Met Ala Ser Leu Asp Gln Met He Phe Gly Thr Ala Leu Pro 15 55 60 65 aca atc gtc ggt gaa ctc ggc ggc gtt gac cac atg atg tgg gtc atc 355 Thr He Val Gly Glu Leu Gly Val Valid Asp His Met Met Trp Val He 70 75 80. "85 acc gca tac cta ctt gcc gaa acc atc atg ctg ccg atc tac gga aag 403 Thr Wing Tyr Leu Leu Wing Glu T hr He Met Leu Pro He Tyr Gly Lys • 90 95 100 ctc ggc gac ctg gtt gga cgt aaa ggt ctc ttc atc gga gcc ctc ggc 451 Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe He Gly Ala Leu Gly 20,105,110,115 atc ttc ctg atc ggc aka gtc atc ggc ggg ctt gca gga aat atg acc 499 He Phe Leu He Gly Ser Val He Gly Gly Leu Ala Gly Asn Met Thr 120 125 130 tgg ttg atc gtc ggc cgt gcc gta cag ggc atc ggt ggc ggt gga ctg 547 Trp Leu He Val Gly Arg Ala Val Gln Gly He Gly Gly Gly Gly Leu 135 140 145 atg atc ctc tcg cag gca atc gcg gac gtt gtt cca gca cgt gaa 595 Met He Leu Ser Gln Ala He He Ala Asp Val Val Pro Ala Arg Glu 25 150 155 160 165 cgt ggc cgc tac atg ggt gtc atg ggt gga gtc ttc gga ctc tct gca 643 Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val Phe Gly Leu Ser Ala 170 175 180 gtt ctt ggc cca cta ggt ggc tgg ctc gaa ttc gga acc ggc tgg cca 691 Val Leu Leu Gly Gly Pro Leu Gly Phe Trp Thr Gly Pro Gly Trp Glu 185 190 195 cgc tgg tgg gca ttc ctg atg aac cca atc atc gcc gga atc ggt at'c 739 A rg Trp Wing Phe Trp Met Asn He Pro Leu Gly He He Wing He Gly 200 205 210 gtc gcc att tac ttc ctg gac att cca aag aag age gtc aag ttc cgc 787 Val Ala He Tyr Phe Leu Asp He Pro Lys Lys Ser Val Lys Phe Arg 215 220 225 tgg gat tac ctg ggc act ttc ttc atg atc gtt gcc gca acc age ctg 835 Trp Asp Tyr Leu Gly Thr Phe Phe Met He Val Wing Wing Thr Ser Leu 230 235 240 245 atc ctg ttc acc acc tgg ggt gga gga tc cg tac gag tgg tct gat cca 883 He Le He Phe Thr Thr Trp Gly Gly Ser Gln Tyr Glu Trp Ser Asp Pro 250 255 260 atc atc att gga ctg atc atc acc atc gtt gcc gcc gc gc ctg ctg 931 He He He Gly Leu He He Thr Thr He Val Ala Ala Ala Leu Leu 265 270 275 gtt gtt gtg gaa ctg cgc gca aaa gat cca ttg gtt cca atg tcc ttc 979 Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu Val Pro Met Ser Phe 280 285 290 ttc caa aac CGC aac ttc ACG ctc acc acc att GCA GGC CTG atc CTG 1027 Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr I Ala Gly Leu He Leu 295 300 • 305 GGT atc GCA atg ttc GGC atc atc GGC tac ctt ccg acc tac ctc cag 1075 Gly He Wing Met Phe Gly He He Gly Tyr Leu Pro Thr Tyr Leu Gln 310 315 320 '325 atg gtc cac gga atc aac gcc acc gaa gcc ggc tac atg ctg atc cca 1123 Met Val His Gly He Asn Wing Thr Glu Wing Gly Tyr Met Leu He Pro 330 335 340 atg atg gtc ggc atg atg ggt acc tcc atc tgg act ggt atc cgc atc 1171 Met Met Val Gly Met Met Gly Thr Ser He Trp Thr Gly He Arg He 345 350 355 tcc aac aca gga aag tac aaa ctc tcc cca cca atc ggc-atg gtg gtt 1219 Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro He Gly Met Val Val 360 365 370 acc ttc gtg gca ctg atc ttc ttt gcc cga atg gaa gtg tcc acc acc 1267 Thr Phe Val Ala Leu He Phe Phe Ala Arg Met Glu Val Ser Thr Thr 375 380 385 U nii I IHI'IIÉ IIIIIII UMÉÉG ctg tgg cag atc gga atc tac ctc gtc gtc ggc ggc ctg ggt 1315 Leu Trp Gln He Gly He Tyr Leu Phe Val Leu Gly Val Gly Leu Gly 390 395 400 405 cta gcc atg cag gtt ctg gtc ctg atc gtt cag aac acc ctg cca acc 1363 Leu Wing Met Gln Val Leu Val Leu He Val Gln Asn Thr Leu Pro Thr 410 415 420 gcg gtg gtc gga tcc gc gc acc gct gc aac aac tcc tcc tc cc at ca 1411 Wing Val Val Gly Ser Wing Thr Wing Val Asn Asn Phe Phe Arg Gln He 425 430 435 ggt tcc tea ctc gga tcc gcg ctg gtc ggt ggc atg ttc gtt ggc aac 1459 Gly Ser Ser Leu Gly Ser Ala Leu Val Gly Gly Me't Phe Val Gly Asn 440 445 450 ttg gga acc ctc atg gaa gaa aga atg cca gca gcc atg gca caa ctt 1507 Leu Gly Thr Leu Met Glu Glu Arg Met Pro Ala Ala Ala Ala Gln Leu 455 460 465 tea cca gaa gaa caa gcc gcc atg gca gcc caa ggc gga ctg gac tcc 1555 Ser Pro Glu Glu Gln Wing Ala Wing Wing Gln Gly Gly Leu Asp Ser 470 475 480 485 aac gaa ttg acg ccg gca atc gtc aat caa ttg cca acc gcg ctc cac 1603 Asn Glu Leu Thr Pro Wing He Val Asn Gln Leu Pro Thr Wing Leu His 490 495 500 gat gcg ttc gcc ggt tcc tac aac gac gca ctc atc cca gtg ttc tac 1651 Asp Wing Phe Wing Gly Ser Tyr Asn Asp Wing Leu He Pro Val Phe Tyr 505 510 515 gtg atg atg cca ctg atc ggc atc gcg ctg ctt ctc ttg ctg ttt att 1699 Val Met Met Pro Leu He Gly He Wing Leu Leu Leu Leu Leu Phe He 520 525 530 aag caa gaa aaa cta cgc gaa acc acc aca gac taaacacaaa acaaatgaga 1752 Lys Gln Glu Lys Leu Arg Glu Thr Thr Thr Asp 535 540 ect 1755 < 210 > 254 < 211 > 544 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 254 Met Thr His Glu Thr Ser Val Pro Gly Pro Wing Asp Wing Gln Val Wing 1 5 10 15 Gly Asp Thr Lys Leu Arg Lys Gly Arg Ala Lys Lys. Glu Lys Thr Pro 20 25 30 Being Ser Met Thr Pro Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser 35 40 45 > j i rVéitá m J t mmm? ? i ^ ^? ^^^^^^^^^^^^^^^^^^^^^ Wing Leu Met Val Wing Met Met Met Wing Being Leu Asp Gln Met He Phe 50 55 60 Gly Thr Ala Leu Pro Thr He Val Gly Glu Leu Gly Val Gly Asp His 65 70 75 80 Met Met Trp Val He Thr Wing Tyr Leu Leu Wing Glu Thr He Met Leu 85 90 95 Pro He Tyr Gly Lys Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe 100 105 110 He Gly Ala Leu Gly He Phe Leu He Gly Ser Val He Gly Gly Leu 115 120 125 Ala Gly Asn Met Thr Trp Leu He Val Gly Arg Ala Val Gln Gly He 130 135 140 Gly Gly Gly Gly Leu Met He Leu Ser Gln Wing He He Wing Asp Val 145 150 155 160 Val Pro Ala Arg Glu Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val 165 170 175 Phe Gly Leu Ser Wing Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr 180 185 190 Glu Gly Pro Gly Trp Arg Trp Wing Phe Trp Met Asn He Pro Leu Gly 195 200 205 He He Wing He Gly Val Wing He Tyr Phe Leu Asp He Pro Lys Lys 210 215 220 Ser Val Lys Phe Arg Trp Asp Tyr Leu Gly Thr Phe Phe Met He Val 225 230 235 240 Wing Wing Thr Ser Leu He Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr 245 250 255 Glu Trp Ser Asp Pro He He He Gly Leu He He Thr He Thr He Val 260 265 270 Ala Ala Ala Leu Val Val Glu Leu Arg Ala Lys Asp Pro Leu 275 280 285 Val Pro Met Ser Phe Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr He 290 295 300 Wing Gly Leu He Leu Gly He Wing Met Phe Gly He He Gly Tyr Leu 305 310 315 320 Pro Thr Tyr Leu Gln Met Val His Gly He Asn Wing Thr Glu Wing Gly 325 330 '335 Tyr Met Leu He Pro Met Met Val Gly Met Met Gly Thr Ser He Trp 340 345 350 > - *% > - - t 'T tiTtoS ^^ aifca ^^^^^^ aBa ^^^^^ M ^ MM ^^^^^ Thr Gly He Arg He Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro 355 360, 365 He Gly Met Val Val Thr Phe Val Ala Leu He Phe Phe Ala Arg Met 370 375 380 Glu Val Ser Thr Thr Leu Trp Gln He Gly He Tyr Leu Phe Val Leu 385 390 395 400 Gly Val Gly Leu Gly Leu Ala Met Gln Val Leu Val Leu He Val Gln 405 410 415 Asn Thr Leu Pro Thr Wing Val Val Gly Ser Wing Thr Wing Val Asn Asn 420 425 430 Phe Phe Arg Gln He Gly Ser Ser Leu Gly Ser Wing Leu Val Gly Gly 435 440 '445 Met Phe Val Gly Asn Leu Gly Thr Leu Met Glu Glu Arg Met Pro Wing 450 455 460 Wing Met Wing Gln Leu Ser Pro Glu Gln Gln Wing Wing Met Wing Ala Gln 465 470 475 480 Gly Gly Leu Asp Being Asn Glu Leu Thr Pro Wing He Val Asn Gln Leu 485 490 495 Pro Thr Ala Leu His Asp Ala Phe Ala Gly Ser Tyr Asn Asp Ala Leu 500 505 510 He Pro Val Val The Val Met Met Pro Leu He Gly He Ala Leu Leu 515 520 525 Leu Leu Leu Phe He Lys Gln Glu Lys Leu Arg Glu Thr Thr Thr Asp 530 535 540 < 210 > 255 < 211 > 1294 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1294) < 223 > FRXA01319 < 400 > 255 ccctgacacc aatcttcttg gcgctcaccc cctcgcagt agtcgccgcg atcctcctct 60 ttttcatccg tgaagatcac ctcaaggaaa cgcacgaata atg aca cac gaa act 115 Met Thr His Glu Thr 1 5 tcc gtc ccc gga ect gcc gac gcg cag gtc gca gga gat acg aag ctg 163 Ser Val Pro Gly Pro Ala Asp Ala Gln Val Ala Gly Asp Thr Lys Leu 10 15 20 cgc aaa ggc cgc gcg aag aag aa aa gaa aaa act ect tea tea atg acg ect 211 Arg Lys Gly Arg Ala Lys Lys Glu Lys Thr Pro Ser Ser Met Thr Pro 25 30 35 gaa caa caa aag aaa gtc tgg tgg ttc gtc ctc age gcg ctg atg gtc gcc 259 Met Met Met Ala Ser Leu Asp Gln Met He Phe Gly Thr Ala Leu Pro 55 60 65 aca atc gtc ggt gaa ctc ggc ggc gtt gac cac atg tgt gtc atc 355 Thr He Val Gly Glu Leu Gly Val Asp His Met Met Trp Val He 70 75 80 85 acc gca tac cta ctt gcc gaa acc atc ctg ccg atc tac gga aag 403 Thr Wing Tyr Leu Leu Wing Glu Thr He Met Leu Pro He Tyr G ly Lys 90 95 100 ctc ggc gac ctg gtt gg cgt aaa ggt ctc ctc atc gga gcc ctc ggc 451 Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe He Gly Ala Leu Gly 105 110 115 atc ttc ctg atc ggc tcc gtc atc ggc ggg ctt gca gga aat atg acc 499 He Phe Leu He Gly Ser Val He Gly Gly Leu Wing Gly Asn Met Thr 120 125 130 tgg ttg atc gtc ggc ggc gg gta cag ggc atc ggt ggt ggt gga ctg 547 Trp Leu He Val Gly Arg Wing Val Gln Gly He Gly Gly Gly Gly Leu 135 140 145 atg atc ctc tcg cag gca atc atc gcg gac gtt gtt cca gca cgt gaa 595 Met He Leu Ser Gln Wing He He Wing Asp Val Val- Pro Wing Arg Glu 150 155 160 165 cgt ggc cgc tac atg ggt gtc atg ggt ggt gtc ttc gga ctc tct gc 643 Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val Phe Gly Leu Ser Wing 170 175 180 gtt ctt ggc cca cta ctc ggt ggc tgg ttc acc gaa gga cca ggc tgg 691 Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr Glu Gly Pro Gly Trp 185 190 195 cgc tgg gca ttc tgg atg aac atc cca ctg gga atc gcc atc ggt 739 Arg Trp Wing Phe Trp Met Asn He Pro Leu Gly He He A He Gly 200 205 210 gtc gcc att tac ttc ctg gac att cca aag aag age gtc aag tcc cgc 787 Val Ala He Tyr Phe Leu Asp He Pro Lys Lys Ser Val Lys Phe Arg 215 220 225 tgg gat tac ctg ggc act ttc ttc atg atc gtt gcc gca acc age ctg 835 Trp Asp Tyr Leu Gly Thr Phe Phe Met He Val Wing Wing Thr Ser Leu 230 235 240 245 - ^ riaMMHHMMB. ^ MaMMMkAHM ^ MHMHMMMM, ^ MMBÍftM ^ atc ctg ttc acc acc tgg ggt gga tcc cag tac gag 'tgg tct gat cca 883 He Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr Glu Trp Ser Asp Pro 250 255 260 atc atc att gga ctg atc atc action acc atc gtt gcc gct gca ctg ctg 931 He He He He Gly Leu He He Thr Thr He Val Ala Ala Ala Leu Leu 265 270 275 gtt gtt gtg gaa ctg cgc gca aaa gat cca ttg gt't cca atg tcc ttc 979 Val Val Val Glu Leu Arg Ala Lys Asp Pro Leu Val Pro Met Ser Phe 280 285 290 ttc caa aac cgc aac ttc acg ctc acc action att gca ggc ctg atc ctg 1027 Phe Gln Asn Arg Asn Phe Thr Leu Thr Thr He Wing Gly Leu He Leu 295 300 305 ggt atc gca atg ttc ggc atc atc ggc tac ctt ccg acc tac ctc cag 1075 Gly He Ala Met Phe Gly He He Gly Tyr Leu Pro Thr Tyr Leu Gln 310 315 320 325 10 atg gtc cac gga atc aac gcc acc gaa gcc gcc tac atg ctg atc cca 1123 Met Val His Gly He Asn Wing Thr Glu Wing Gly Tyr Met Leu He Pro 330 335 340 atg atg gtc ggc atg atg ggt acc atc tgg act ggt atc cgc atc 1171 Met Met Val Gly Met Met Gly Thr Ser He Trp Thr Gly He Arg He 345 350 355cc aac aca gga aag tac aaa ctc ttc cca cca cca atc ggc atg gtg gtt 1219 Ser Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro He Gly Met Val Val 360 365 370 15 acc ttc gtg gca ctg atc ttc ttt gcc cga atg gaa gtg tcc acc acc 1267 Thr Phe Val Wing Leu He Phe Phe Wing Arg Met Glu Val Ser Thr Thr 375 380 385 ctg tgg cag atc gga atc tac ctc ttc 1294 Leu Trp Gln He Gly He Tyr Leu Phe 390 395 < 210 > 256 < 211 > 398 20 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 256 Met Thr His Glu Thr Ser Val Pro Gly Pro Wing Asp Wing Gln Val Wing 1 5 10 15 Gly Asp Thr Lys Leu Arg Lys Gly Arg Wing Lys Lys Glu Lys Thr Pro 20 25 30 Being Ser Met Thr Pro Glu Gln Gln Lys Lys Val Trp Trp Val Leu Ser 35 40 45 25 - "* A ---- Afa-- Wing Leu Met Val Wing Met Met Met Wing Being Leu Asp Gln Met He Phe 50 55 60 Gly Thr Wing Leu Pro Thr He Val Gly Glu Leu Gly Gly Val Asp His 65 70 75 80 Met Met Trp Val He Thr Ala Tyr Leu Leu Ala Glu Thr He Met Leu 85 90 95 Pro He Tyr Gly Lys Leu Gly Asp Leu Val Gly Arg Lys Gly Leu Phe 100 105 110 He Gly Ala Leu Gly He Phe Leu He Gly Ser Val He Gly Gly Leu 115 120 125 Wing Gly Asn Met Thr Trp Leu He Val Gly Arg # Wing Val Gln Gly He 130 135 * 140 Gly Gly Gly Gly Leu Met He Leu Ser Gln Wing He He Wing Asp Val 145 150 155 160 Val Pro Ala Arg Glu Arg Gly Arg Tyr Met Gly Val Met Gly Gly Val 165 170 175 Phe Gly Leu Ser Ala Val Leu Gly Pro Leu Leu Gly Gly Trp Phe Thr 180 185 190 Glu Gly Pro Gly Trp Arg Trp Ala Phe Trp Met Asn He Pro Leu Gly 195 200 205 He He Ala He Gly Val Ala He Tyr Phe Leu Asp He Pro Lys Lys 210 215 220 Ser Val Lys Phe Arg Trp Asp Tyr Leu Gly Thr Phe Phe Met He Val 225 230 235 240 Ala Ala Thr Ser Leu He Leu Phe Thr Thr Trp Gly Gly Ser Gln Tyr 245 250 255 Glu Trp Ser Asp Pro He He He Gly Leu He He Thr He Thr He Val 260 265 * 270 Ala Ala Ala Leu Val Val Le Val Vallu Glu Leu Arg Wing Lys Asp Pro Leu 275 280 285 Val Pro Met Be Phe Phe Gln Asn Arg Asn Phe Thr 'Leu Thr Thr He 290 295 300 Wing Gly Leu He Leu Gly He Wing Met Phe Gly He He Gly Tyr Leu 305 310 315 320 Pro Thr Tyr Leu Gln Met Val His Gly He Asn Wing Thr Glu Wing Gly 325 330 335 Tyr Met Leu He Pro Met Met Val Gly Met Met Gly Thr Ser He Trp 340 345 '350 r. .i m i? ? . ••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• ??? Thr Gly He Arg He Being Asn Thr Gly Lys Tyr Lys Leu Phe Pro Pro 355 360 365 He Gly Met Val Val Thr Phe Val Ala Leu He Phe -Phe Ala Arg Met 370 375 380 Glu Val Ser Thr Thr Leu Trp Gln He Gly He Tyr Leu Phe 385 390 395 < 210 > 257 < 211 > 1510 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (93) .. (1487) < 223 > RXA01578 < 400 > 257 tgacctcccc tcaaccactc actgaaaaga gaacactgat gtcacttcct gttcagccga 60 gtaaaacctc ggccgccaca gtcataccat tg atg atc gcc ctg ctg gtc gcg 113 Met He Ala Leu Leu Val Ala 1 5 gta ttc gcc ttc cag ctc aac gcc tcc atg ctg gcg ccg gca ctg gcc 161 Val Phe Ala Phe Gln Leu Asn Ala Ser Met Leu Ala Pro Ala Leu Ala October 15 20 acc atg gaa act gaa ctt aat gca aca gct gcc caa atc ggc atg acg 209 Thr Met Glu Thr Glu Leu Asn Ala Thr Ala Ala Gln 'He Gly Met Thr 25 30 35 cag act gct ttc ttc acc gcg gcg gcg ctg ttt aka ctg ttc ctg cca 257 Gln Thr Ala Phe Phe Thr Ala Ala Ala Leu Phe Ser * Leu Phe Leu Pro 40 45 50 55 cgt tgg ggc gat ctg att ggt cgc cgc aaa gtg ctg gtc ggc atg atg 305 Arg Trp Gly Asp Leu He Gly Arg Arg Lys Val Leu Val Gly Met Met 60 65 70 att gtc acc ggt att gga tgt gtt gtc gct gcc ttt gct ccg aat gtg 353 He Val Thr Gly He Gly Cys Val Val Ala Ala Phe Ala Pro Asn Val 75 80 85 acc atc ctc ttc ctg ggc cgc ctg att caa ggt gtt 'gct ggc cca acc 401 Thr He Leu Phe Leu Gly Arg Leu He Gln Gly Val A Gly Pro Thr 90 95 100 gtg cca ctg tgt ctg atc att ctg cgc cag cag gta acc aat gaa aag 449 Val Pro Leu Cys Leu He He Leu Arg Gln Gln Val Thr Asn Glu Lys 105 110 115 caa tat gcg cta ctt ctc gga att gtt acc tct gtc. aac ggt ggt atc 497 Gln Tyr Ala Leu Leu Leu Gly He Val Thr Ser Val Asn Gly Gly He "Ti i ^ - ^ - ^^^ ..) ^ 120 125 130 135 ggc ggc gtg gac gcg ctt gct ggt ggc tgg ttg gct gaa aca ctt ggt 545 Gly Gly Val Asp Wing Leu Wing Gly Gly Trp Leu Wing Glu Thr Leu Gly 140 145 150 ttc ct tc atc ttc tgg gtc tgc atg gct gct ttc tgc gct gtc gct gcc 593 phe arg ser hep phe trp Val Met Wing Ala Phe Cys Ala Ala Ala Ala 155 160 165 ctc gca ctg ect ttc age gtg aag gaa tcc acc gct gaa gac acc ccg 641 Leu Ala Leu Pro Phe Ser Val Lys Glu Ser Thr Ala Glu Glu Thr Pro 170 175 180 aag atg gac tgg ctt ggt gtg ctg cca ctg gcg gtg tcc att gga tct 689 Lys Met Asp Trp Leu Gly Val Leu Pro Leu Wing Val Ser He Gly Ser 185 190 195 ttg ctc atg gct tcc aac gacc gcc gcc aaa ctc ggt gcc gcg aac tgg 737 Leu Leu Met Wing Phe Asn Glu Wing Gly Lys Leu Gly Wing Wing Asn Trp 200 205 210 215 att ctg gtg gtt gtg ctg ttc atc atc ggt atc gcc gga gtc atc ttc 785 He Leu Val Val Val Leu Phe He He Gly He Wing Gly Val He Phe 220 225 230 ttc tac aac att gaa aag cgc gtt aag cac ccg ctg gtc agt gtt gaa 833 Phe Tyr Asn He Glu Lys Arg Val Lys His Pro Leu Val Ser Val Glu 235 240 245 tac ctc ggt caa cga cgc acc tgg gca ttg ctg ct g age acc ctt ctc 881 Tyr Leu Gly Gln Arg Arg Thr Trp Wing Leu Leu Leu Ser Thr Leu Leu 250 255, 260 aca atg acc ggt gta ttc gcc gta atg aat ggt ctg ctg ccc aac ctt 929 Thr Met Thr Gly Val Phe Ala Val Met Asn Gly Leu Pro Asn Leu 265 270 275 gcg cag gat gct gcc aac ggt gcc ggc atg tea gcgt age gtg gtg tcc 977 Wing Gln Asp Wing Wing Asn Gly Wing Gly Met Wing Ser Wing Val Val Ser 280 285 290 295 tgg tgg tgg tc ct tat cc tat cc gcg ctg gct gcc ttg gta ttc gcc cca 1025 Trp Trp Thr Leu Thr Pro Tyr Ala Leu Wing Gly Leu Val Phe Gly Pro 300 305 310 atc gcc gt gta akt ctc gcc gga aaa ttt gga tac aag atc gtc ctg caa 1073 He Wing Gly He Leu Wing Gly Lys Phe Gly Tyr Lys He Val Leu Gln 315 320, 325 att ggt atc gct gcc acc atc atc ggc gtt gcc gcc acc ttc tta 1121 He Gly He Wing Wing Thr He He Gly Val Wing Gly Wing Thr Phe Leu 330 335 340 gtc gga age acc tcg cat ctc gcg tac ctc ggc atc tcc atc ttc gtg 1169 Val Gly Ser Thr Ser His Leu Wing Tyr Leu Gly He Ser He Phe Val 345 350 355 ggt att tat tat gg ggt att gcc aac atc atc ctc aac ggc ctg ggc 1217 Gly He Thr Tyr Wing Gly He Wing Asn He Met Leu Asn Gly L eu Gly 360 365 370 375 atc gtg ctc tcc ect gct aac aac aac aa ggc tat ctg ect ggc at aa aac 1265 He Val Leu Ser Pro Wing Asn Asn Gln Gly Tyr Leu Pro Gly Met Asn 380 385 390 gca ggt gcc ttc aac cta ggt gca gca ggt att tcc ttc gcc atc ctc ctc 1313 Gly Wing Wing Phe Asn Leu Gly Wing Gly He Ser Phe Wing He Leu Phe 395 400 405 gca gtt tcc acg gca ttc agt gac aac ggc gga gga tac gcc gca gcc 1361 Wing Val Ser Thr Wing Phe Ser Asp Asn Gly Gly Gly Tyr Ala Wing Gly 410 415 420 atg tgg ggc ggc atc gtc atc ttg gtc cta gcc ttc ctc tgc tcc ctg 1409 Met Trp Wing Gly Val He He Leu Val Leu Wing Phe Leu Cys Ser Leu 425 430 435 ctg atc cca cgc cca gaa tea atc acc gat gtg gca gc gcc aaa gtc 1457 Leu He Pro Arg Pro Glu Be He Thr Asp Thr Val Ala Wing Lys Val 440 445 450 455 cag gct gaa gaa gcc gct caa gcc gcc age taaatccaca aactgaacta 1507 Gln Ala Glu Glu Ala Ala Gln Ala Ala Ser 460 465 agg 1510 < 210 > 258 < 211 > 465 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 258 Met He Ala Leu Leu Val Ala Val Phe Ala Phe Gln Leu Asn Ala Ser 1 5 10. 15 Met Leu Ala Pro Ala Leu Ala Thr Met Glu Thr Glu Leu Asn Wing Thr 20 25 30 Wing Wing Gln He Gly Met Thr Gln Thr Wing Phe Phe Thr Wing Wing Wing 35 40 45 Leu Phe Ser Leu Phe Leu Pro Arg Trp Gly Asp Leu He Gly Arg Arg 50 55 60 Lys Val Leu Val Gly Met Met He Val Thr Gly He Gly Cys Val Val 65 70 75 80 Wing Wing Phe Wing Pro Asn Val Thr He Leu Phe Leu Gly Arg Leu He 85 90 95 Gln Gly Val Wing Gly Pro Thr Val Pro Leu Cys Leu He He Leu Arg íé j .1. r. . , -, i ~ ?. .. 100 105 110 Gln Gln Val Thr Asn Glu Lys Gln Tyr Ala Leu Leu Leu Gly He Val 115 120 125 Thr Ser Val Asn Gly Gly He Gly Gly Val Asp Ala Leu Wing Gly Gly 130 135 140 • Trp Leu Wing Glu Thr Leu Gly Phe Arg Ser He Phe Trp Val Met Wing 145 150 155 '160 Wing Phe Cys Wing Wing Wing Wing Leu Wing Leu Pro Phe Ser Val Lys Glu 165 170 175 Wing Thr Wing Glu Glu Thr Pro Lys Met Asp Trp Leu Gly Val Leu Pro 180 185 190 Leu Ala Val Ser He Gly Ser Leu Leu Met Ala Phe Asn Glu Ala Gly 195 200 205 10 Lys Leu Gly Ala Ala Asn Trp He Leu Val Val Val Leu Phe He He 210 215 220 Gly He Wing Gly Val He Phe Phe Tyr Asn He Glu Lys Arg Val Lys • 225 230 235 240 His Pro Leu Val Ser Val Glu Tyr Leu Gly Gln Arg Arg Thr Trp Wing 245 250 255 Leu Leu Leu Ser Thr Leu Leu Thr Met Thr Gly Val Phe Wing Val Met 260 265 270 5 Asn Gly Leu Leu Pro Asn Leu Wing Gln Asp Wing Wing Asn Gly Wing Gly 275 280 285 Met Ser Wing Being Val Val Ser Trp Trp Thr Leu Thr Pro Tyr Ala Leu 290 295 300 'Wing Gly Leu Val Phe Gly Pro He Wing Gly He Leu Wing Gly Lys Phe 305 310 315 - 320 Gly Tyr Lys He Val Leu Gln He Gly He Wing Wing Thr He He Gly 325 330 335 20 Val Wing Gly Wing Thr Phe Leu Val Gly Ser Thr S er'-His Leu Ala Tyr 340 345 350 Leu Gly He Ser He Phe Val Gly He Thr Tyr Wing Gly He Wing Asn 355 360 '365 He Met Leu Asn Gly Leu Gly He Val Leu Ser Pro Wing Asn Asn Gln 370 375 380 Gly Tyr Leu Pro Gly Met Asn Wing Gly Wing Phe Asn Leu Gly Wing Gly 385 390 395 400 25 He Be Phe Wing He Leu Phe Wing Val Being Thr Wing Phe Being Asp Asn tftaaMaaflttMMMaillÁrih ^ 405 410 415 Gly Gly Gly Tyr Wing Wing Gly Met Trp Wing Gly Val He He Leu Val 420 425 430 Leu Wing Phe Leu Cys Ser Leu Leu He Pro Arg Pro Glu Ser He Thr 435 440 445 Asp Thr Val Wing Wing Lys Val Gln Ala Glu Glu Ala Ala Gln Ala Ala 450 455 460 Ser 465 < 210 > 259 < 211 > 1470 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1447) < 223 > RXA02087 < 400 > 259 aatcggattc atgctgtgtg gtgtgatcag tttgctggct gcggtcgcat ggatcttcgg 60 ccgggagacg ctgccaacgg cgaaagtcga gcaggtataa atg acg cca att gtg 115 Met Thr Pro He Val 1 5 gag tcc agg gcg tgg aaa gct ctg ggc gct tta agt 'gtt ggg ctg ttt 163 Glu Ser Arg Ala Trp Lys Ala Leu Gly Ala Leu Ser.'Val Gly Leu Phe 10 15 20 ctc aca ctg ctt gac caa tcg ttg gtg gct gtc gcg ctg cca aag att 211 Leu Thr Leu Leu Asp Gln Ser Leu Val Ala Val Ala Leu Pro Lys He 25 30 35 caa gag gat ttg ggt gcg age ctg aac caa gcg gtg tgg gtg tea gcg 259 Gln Glu Asp Leu Gly Ala Ser Leu Asn Gln Ala Val '. Trp Val Ser Wing 40 45 50 gtt tat ttg ctc act ttt gcg gtg cca ctg ttg att act ggg cgc ttg 307 Val Tyr Leu Leu Thr Phe Ala Val Pro Leu Leu He Thr Gly Arg Leu 55 60 65 ggt gat cgt tat gga cag cga aat att tat ctt gcc ggc atg gct gtg 355 Gly Asp Arg Tyr Gly Gln Arg Asn He Tyr Leu Wing Gly Met Wing Val 70 75 80 85 ttt acc ctc gcg gcg ttg gcc tgt gta ttt gca cca age atc gaa tgg 403 Phe Thr Leu Ala Ala Leu Ala Cys Val Phe Pro Pro Wing He Glu Trp 90 95 100 ttg att gct gct cgc gcg gtg cag ggc ctg ggc gga tct ctt ctt aat 451 Leu He Ala Ala Arg Ala Val Gln Gly Leu Gly Gly Ser Leu Leu Asn 105 110 115 ccg cag ccc ctg age atc att cac aag att ttc gcg cat gat cgt agg 499 Pro Gln Pro Leu Ser He He He His Lys He Phe Wing His Asp Arg Arg 120 125 130 gga gcc gcc ggg ggg tgg agg gtt gtt gtt tea tea gct gga gtt ctt 547 Gly Ala Ala Thr Gly Val Trp Ser Ala Val Ala Ser Ser Wing Gly Leu 135 140 145 ttc ggg cca gtt atc ggt ggt gtt gtg gtg ggg tgg atc age tgg cgt 595 Phe Gly Pro Val He Gly Gly Val Leu Val Gly Trp He Ser Trp Arg 150 155 160 165 gct gtg ttc ttg gtt tat gtg ccg ctc gga ttg atc tcc cta ttt atg 643 Wing Val Phe Leu Val Tyr Val Pro Leu Gly Leu He Ser Leu Phe Met 170 175 180 gtg gcg tat gtg ect aaa ctt ccc acg gga acc tcg aag atc gat 691 Val Ala Arg Tyr Val Pro Lys Leu Pro Thr Gly Thr Ser Lys He Asp 185 190 195 tgg ctc tcg ggt gcg gtc tea ctt gtt gtt gta ctt ggt gtt gtt ctt 739 Trp Leu Ser Gly Ala Val Ser Leu Val Ala Val Leu Gly Val Val Leu 200 205 2 10 gcc tg gg tg gg cca ggg cg ggg cgg ggg tgg tg ggg tg ggg 787 Wing Gln Gln Gly Pro Glu Leu Gly Trp Gly Thr Leu He Trp Val 215 220 225 tcc ctt gtt gtt gtt ggt att gct gct gtg ctc ttt ata tgg atg caa 835 Ser Leu Wing Val Gly He Wing Wing Wing Val Leu Phe He Trp Met Gln 230 235 240 245 here aga aac gcg cca ctg atg ccg ttg agg att ttc aag acg cgc 883 Thr Arg Ser Lys Wing Pro Leu Met Pro Leu Arg He Phe Lys Thr Arg 250 255 260 aac ttc gcg atc ggt gca ttt tcg atc ttc age ctg ggc ttt acg gtg 931 Asn Phe Wing He Gly Wing Phe Ser He Phe Ser Leu .Gly Phe Thr Val 265 270 275 tat tcc gtt aat ttg ccc atc ttg tat ctg caa acg gct cag gga 979 Tyr Ser Val Asn Leu Pro He Met Leu Tyr Leu Gln Thr Wing Gln Gly 280 285 • 290 atg tcg tcg cag tcg gcc tgt tg atg ttg gtt ccg atg ggc atc atc 1027 Met Ser Ser Gln Leu Wing Gly Leu Met Leu Val Pro Met Gly He He 295 300 305 tct gtg gtg atg tea cca gta att gga cga ttg gtg gat cgc ctg gca 1075 Ser Val Val Met Ser Pro Val He Gly Arg Leu Val Asp Arg Leu Wing 310 315 320 325 cca gga atg atc tcc aag atc gga ttc ggc gcg ctg att ttc tcg atg 1123 Pro Gly Met He Ser Lys He Gly Phe Gly Ala Leu He Phe Ser Met 330 335 340 gcg ttg atg gct gtc ttt atg atc gcc aac cta tcg ccg tgg tgg cta 1171 Ala Leu Met Ala Val Phe Met He Ala Asn Leu Se r Pro Trp Trp Leu 345 350 355 ctc atc ccg att att ttc ggt age tcc aac gcg atg agt ttt gca 1219 Leu He Pro He He Leu Phe Gly Ser Ser Asn Ala Met Ser Phe Wing 360 365 370 ccg aac tct gtg att gct ctg cgt gat gtt ccg cag gat tta gtg ggc 1267 Pro Asn Ser Val He Wing Leu Arg Asp Val Pro Gln Asp Leu Val Gly 375 380 385 tct gct tct ggt ttt tac aac acc teacgc cag gtg ggc gct gtt ttg 1315 Ser Ala Ser Gly Phe Tyr Asn Thr Ser Arg Gln Val Gly Wing Val Leu 390 395 400 405 ggc gcc gct gtc acc gtc tcg gct gtg atg caa gga gtg ata ggc acg gtg 1363 Gly Wing Wing Thr Leu Gly Wing Val Met Gln He Gly Val Gly Thr Val 410 415 420 tcc ttc ggt gtt gcc atg ggt gcg gca atc ctg gtg aca ctc gtg ccc 1411 Ser Phe Gly Val Ala Met Gly Ala Ala He Leu Val Thr Leu Val Pro 425 430 435 tta atc ttt ggg ttc cta gcg gta acc ata ttt aga tagttgct.ee 1457 Leu He Phe Gly Phe Leu Wing Val Thr Gln Phe Arg 440 445 gattttctca cga 1470 < 210 > 260 < 211 > 449 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 260 Met Thr Pro He Val Glu Ser Arg Ala Trp Lys Ala Leu Gly Ala Leu 1 5 10. 15 Ser Val Gly Leu Phe Leu Thr Leu Leu Asp Gln Ser Leu Val Wing Val 20 25 30 Wing Leu Pro Lys He Gln Glu Asp Leu Gly Wing Being Leu Asn Gln Wing 35 40 45 Val Trp Val Ser Wing Val Tyr Leu Leu Thr Phe Wing Val Pro Leu Leu 50 55 60 He Thr Gly Arg Leu Gly Asp Arg Tyr Gly Gln Arg Asn He Tyr Leu 65 70 75 • 80 Wing Gly Met Wing Val Phe Thr Leu Wing Wing Leu Wing Cys Val Phe AYa 85 90 95 Pro Ser He Glu Trp Leu He Wing Wing Arg Wing Val Gln Gly Leu Gly 100 105 110 Gly Ser Leu Leu Asn Pro Gln Pro Leu Ser He He His Lys He Phe 115 120 125 Wing His Asp Arg Arg Gly Wing Wing Thr Gly Val Trp Wing Val Ala 130 135 140 «Be Ser Wing Gly Leu Phe Gly Pro Val He Gly Val Val Val Val Gly 145 150 155 160 Trp He Ser Trp Arg Ala Val Phe Leu Val Tyr Val P.ro Leu Gly Leu 165 170 175 He Be Leu Phe Met Val Wing Arg Tyr Val Pro Lys Leu Pro Thr Gly 180 185 190 Thr Ser Lys He Asp Trp Leu Ser Gly Wing Val Ser Leu Val Wing Val 195 200 205 Leu Gly Val Val Leu Ala Leu Gln Gln Gly Pro Glu Leu Gly Trp Gly 210 215 220 Thr Leu He Trp Val Ser Leu Wing Val Gly He Wing Wing Wing Val Leu 225 230 235 240 Phe He Trp Met Gln Thr Arg Ser Lys Wing Pro Leu Met Pro Leu Arg 245 250 255 He Phe Lys Thr Arg Asn Phe Wing He Gly Wing Phe Ser He Phe Ser 260 265 270 Leu Gly Phe Thr Val Tyr Ser Val Asn Leu Pro He Met Leu Tyr Leu 275 280 285 Gln Thr Wing Gln Gly Met Ser Ser Gln Leu Wing Gly Leu Met Leu Val 290 295 300 Pro Met Gly He He Ser Val Val Met Ser Pro Val He Gly Arg Leu 305 310 315 320 Val Asp Arg Leu Wing Pro Gly Met He Ser Lys He Gly Phe Gly Wing 325 330 335 Leu He Phe Ser Met Ala Leu Met Ala Val Phe Met He Ala Asn Leu 340 345 350 Ser Pro Trp Trp Leu Leu He Pro He He Leu Phe Gly Ser Ser Asn 355 360 365 Wing Met Ser Phe Ala Pro Asn Ser Val He Ala Leu Arg Asp Val Pro 370 375 380 Gln Asp Leu Val Gly Ser Wing Ser Gly Phe Tyr Asn Thr Ser Arg Gln 385 390 395 400 «MMaaÉki Val Gly Wing Val Leu Gly Wing Wing Thr Leu Gly Wing Val Met Gln He 405 410 '415 Gly Val Gly Thr Val Ser Phe Gly Val Wing Ala Gly Ala Wing He Leu 420 425 430 Val Thr Leu Val Pro Leu He Phe Gly Phe Leu Wing Val Thr Gln Phe 435 440 445 Arg < 210 > 261 < 211 > 1338 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1315) < 223 > RXA02088 < 400 > 261 agagcaccca agcgacattg ttegataata tgttctatta ttggtattaa tactggtcag 60 ctagatataa aaggggcgca gggctagcct tgggtgtgaa atg tta act aaa 115 Met Leu Thr Gln Lys 1 5 ata gaa tta gag gct aaa cca aaa atc cca gag gag atc tgg gtg ctg 163 He Glu Leu Glu Ala Lys Pro Lys He Pro Glu Glu He Trp Val Leu 10 15 20 gtt gtg gct gcg ttt att att gcg ctg ggc tat ggc ctg att gcg ccg 211 Val Val Ala Wing Phe He He Wing Wing Leu Gly Tyr Gly Leu He Wing Pro 25 30 35 att ttg cca cag ttt gtg gtc ggt ttt gat gta agt ttt gc gct gcc 259 He Leu Pro Gln Phe Val Val Gly Phe Asp Val Ser Phe Wing Wing Wing 40 45 50 agt gcg gtg gtg tcc atc ttt gcg ggc gcc cgg ttg ttg ttt gcg ccg 307 Be Wing Val Val Be He Phe Wing Gly Wing Arg Leu Leu Phe Wing Pro 55 60 65 atg tcg ggg agt ttg atc gat aag atc ggt tcc cgt cgt gtg tat ctc 355 Met Ser Gly Ser Leu He Asp Lys He Gly Ser Arg Arg Val Tyr Leu 70 75 80 _ 85 act ggt tta ctc acc gtg gct atc acc acg ggg ctt gtt gcg ttg gcg 403 Thr Gly Leu Leu Thr Val Wing Thr Thr Gly Leu Val Wing Leu Al a 90 95 100 cag gaa tac tgg cag att ctg ctg ctt cgt ggc atc gca ggt att ggt 451 Gln Glu Tyr Trp Gln He Leu Leu Arg Gly He Wing Gly He Gly 105 110 115 tcc acc atg ttt acg gtc tct gcc atg ggc ctg atc gtg aag atg gcg 499 Ser Thr Met Phe Thr Val Ser Wing Met Gly Leu He Val Lys Met Wing 120 125 130 ccg gtg gag atc cgc ggg cgg tgt tcg gta tat gcc agt tcg ttc 547 Pro Val Glu He Arg Gly Arg Cys Ser Ser Val Tyr Wing Ser Ser Phe 135 140 145 ctg ttt ggc aat att att ggc ccg gtt gtg ggt gcg gcg atg tcc ggt 595 Leu Phe Gly Asn He He Gly Pro Val Val Gly Ala Wing Met Gly 150 155 160 165 ttg ggc atg cgg tgg ccg ttc gcg att tat ggt gct tcc gtt ggc tta 643 Leu Gly Met Arg Trp Pro Phe Wing He Tyr Gly Wing Wing Val Gly Leu 170 175 180 gct gtt ctt gtt gtg tgg tgg cgg atg ccg aaa acc aac gat tea ctt 691 Wing Wing Leu Val Val Trp Arp Met Pro Lys Thr Asn Asp Ser Leu 185 190 195 cgg aag gct gat age aat agt gtg ccg gcg ttg cge ttt gct gag gca 739 Arg Lys Wing Asp Ser Asn Ser Val Pro Ala Leu Arg Phe Al a Glu Wing 200 205 210 att aag gat tct gcc tac cgc tcg gcg ttg ttt agt gct ttt gcc aat 787 He Lys Asp Ser Ala Tyr Arg Ser Ala Leu Phe Ser Ala Phe Ala Asn 215 220 225 ggt tgg tcg aac ttt ggt gtg cgt gtg gcg gtt ctt cca ctg ttt gcc 35 Gly Trp Ser Asn Phe Gly Val Arg Vai Wing Val Leu Pro Leu Phe Wing 230 235 240 245 gca gct gtt ttt age aat ggc gga gct att gcg gtt ttt gcc atg gct 83 Ala Ala Wing Phe Being Asn Gly Gly Wing Wing Wing Gly Phe Wing Wing 250 255 260 gcg ttc gcc gct gt aat gct ttg tgt ctg caa ttc gcg ggc gat ctc 931 Wing Phe Wing Wing Wing Asn Wing Leu Cys Leu Gln Phe Wing Gly Asp Leu 265 270 275 tea gat cgc att ggc cgt aaa ccg atg att tcc ggg ctg atc gtc 979 Being Asp Arg He Gly Arg Lys Pro Met He He Ser Gly Leu He Val 280 285 290 aat gca gtg ttc acg gca atg atc gga ttc ggc aca gaa gtg tgg atc 1027 Asn Wing Val Phe Thr Wing Met He Gly Phe Gly Thr Glu Val Trp He 295 300 305 ctg atc acg gta tct gcg ttg gca ggt gct ggt gcg ggc ttg ctt aat 1075 Leu He Thr Val Ser Ala Leu Ala Gly Ala Gly Allah Gly Leu Leu Asn 310 315 320 325 cca agt cag gcg gtg gtg ctc gct gat gtt ata gat tcc cgc ccc ggc 1123 Pro Ser Gln Gln Ala Val Leu Ala Asp Val He Asp Ser Arg Pro Gly 330 335 '340 gga aaa gtc tta gcg aat ttc caa atg gcg cag gat ttc ggt gcg att 1171 Gly Lys Val Leu Wing Asn Phe Gln Met Wing Gln Asp Phe Gly Wing He 345 350. _ '355 gtt ggc ccg att ctc gta ggc atg atc gca gaa cag gca gcc ttc caa 1219 Val Gly Pro He Leu Val Gly Met He Wing Glu Gln Wing Gly Phe Gln 360 365 370 atc gga ttc atg ctg tgt ggt gtg atc agt ttg ctg gct gcg gtc gcc 1267 He Gly Phe Met Leu Cys Gly Val He Ser Leu Leu Ala Ala Wing 375 380 385 tgg atc tcc ggc cgg gag acc ccc acc gcg afea gcc gag cag gta 1315 Trp He Phe Gly Arg Glu Thr Leu Pro Thr Ala Lys Val Glu Gln Val 390 395 400 405 taaatgacgc caattgtgga gtc 1338 < 210 >; 262 10 < 211 > 405 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 262 Met Leu Thr Gln Lys He Glu Leu Glu Wing Lys Pro Lys He Pro Glu 1 5 10 15 Glu He Trp Val Leu Val Val Wing Wing Phe He He Wing Leu Gly Tyr 20 25 30 Gly Leu He Wing Pro He Leu Pro Gln Phe Val Val Gly Phe Asp Val 15 35 40 45 Ser Phe Ala Ala Ala Ser Ala Val Val Ser He Phe Ala Gly Ala Arg 50 55 60 Leu Leu Phe Ala Pro Met Ser Gly Ser Leu He Asp Lys He Gly Ser 65 70 75 80 jA Arg Arg Val Tyr Leu Thr Gly Leu Leu Thr Val Wing He Thr Thr Gly ^ 85 90 95 Leu Val Wing Leu Wing Gln Glu Tyr Trp Gln He Leu Leu Aru Gly 20 100 105 110 He Wing Gly He Gly Being Thr Met Phe Thr Val Ser Wing Met Gly Leu 115 120 125 He Val Val Lys Met Pro Wing Val Glu He Arg Gly Arg Cys Ser Ser Val 130 135 140 Tyr Wing Being Ser Phe Leu Phe Gly Asn He He Gly Pro Val Val Gly 145 150 155 160 Wing Wing Met Ser Gly Leu Gly Met Arg Trp Pro Phe Wing He Tyr Gly 25 165 170 175 Wing Being Val Gly Leu Wing Wing Leu Val Val Trp Arp Met Pro Lys 180 185 190 Thr Asn Asp Ser Leu Arg Lys Wing Asp Ser Asn Ser Val Pro Wing Leu 195 200 205 Arg Phe Wing Glu Wing He Lys Asp Being Wing Tyr Arg Be Ala Leu Phe 210 2.5"220 Be Wing Phe Wing Asn Gly Trp Be Asn Phe Gly Val Arg Val Wing Val 225 230 235 240 Leu Pro Leu Phe Wing Wing Wing Phe Being Asn Gly Gly Wing Wing 245 250 255 Gly Phe Wing Met Wing Wing Phe Wing Wing Gly Asn Wing Leu Cys Leu Gln 260 265 270 Phe Wing Gly Asp Leu Being Asp Arg He Gly Arg Lys Pro Met He He 10 275 280 285 Ser Gly Leu He Val Asn Ala Val Phe Thr Ala Met He Gly Phe Gly 290 295 300 Thr Glu Val Trp He Leu He Thr Val Ser Ala Leu Ala Gly Ala Gly 305 310 315 320 Ala Gly Leu Leu Asn Pro Ser Gln Gln Ala Val Leu Ala Asp Val He 325 330 '335 Asp Ser Arg Pro Gly Gly Lys Val Leu Ala- Asn Phe Gln Met Ala Gln 15 340 345 350 Asp Phe Gly Ala He Val Gly Pro He Leu Val Gly Met He Ala Glu 355 360. 365 Gln Ala Gly Phe Gln He Gly Phe Met Leu Cys Gly Val He Ser Leu 370 375 380 Leu Ala Ala Val Ala Trp He Phe Gly Arg Glu Thr Leu Pro Thr Ala 385 390 395 400 Lys Val Glu Gln Val 20 405 < 210 > 263 < 211 > 1239 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1216) < 223 > RXA00764 25 < 400 > 263 tactgcgtcg gatccgctga tgcttgcaga atcggacagt gatgggccgt ctgcgcctgc 60 acctgggacg actggattat taggggtgga attttcgctc atg aca ctc aag act 115 Met Thr Leu Lys Thr 1 5 age gtt ttg gca cta ctc tta gat aac gtg cat gtt ctt ctg att gcg 163 Ser Val Leu Ala Leu Leu Leu Asp Asn Val His Val Leu Leu He Wing 10 15 20 aat ect gag tcg acc acg cag acg cag aaa ctt ttc cgt cgt gtg gtg 211 Asn Pro Glu Ser Thr Thr Gln Thr Gln Lys Leu Phe Arg Arg Val Val 25 30 35 ect gcg ttg atg gcg ctt gat ggt gtg tcg ctt gaa gcg agg ttt acg 259 Pro Ala Leu Met Ala Leu Asp Gly Val Ser Leu Glu Ala Arg Phe Thr 40 45 50 cac tat gga ggc cat gcg gag gag atg gtt ggt gt gtg acg gtg gat 307 His Tyr Gly Gly His Wing Glu Glu Met Val Wing Gly Leu Thr Val Asp 55 60 65 gat ttt gat gtg att atc ccc gcg ggt ggg gac ggc acc gtc aac gaa 355 Asp Phe Asp Val He He Pro Wing Gly Gly Asp Gly Thr Val Asn Glu 70 75 80 85 gtg ata aat ggg tta ctt ggg tcg gcg gaa ggt gat ttt aga aac ctt 403 Val He Asn Gly Leu Leu Gly Ser Wing Glu Gly Asp Phe Arg Asn Leu 90 95 100 gag gat ttg ccg gcg att gcg gtg ttg cca acg ggg tct gcc aat gtg 451 Glu Asp Leu Pro Wing Wing Val Leu Pro Thr Gly Ser Wing Asn Val 105 110 115 ttt gcc cgt gcg ctt ggt tac ccc act gac ccg tat 'gct gcc gct gat 499 Phe Ala Arg Ala Leu Gly Tyr Pro Thr Asp Pro Tyr Wing Wing Asp 120 125 130 gcc ctg gtg gag ttg att cgg aag aac cac acc aga act atc acc ttg 547 Wing Leu Val Glu Leu He Arg Lys Asn His Thr Arg Thr He Thr Leu 135 140 145 ggt acg tgg aag ggt gat gat cag ggg act cgt tgg ttc gcg gtt aat 595 Gly Thr Trp Lys Gly Asp Asp Gln Gly Thr Arg Trp Phe Wing Val Asn 150 155 160 165 gct ggg ttt ggt att gat gcg gat gtt att gcc agg gtc gaa cgg gcg 643 Wing Gly Phe Gly He Asp Wing Asp Val He Wing Arg Val Glu Arg Wing 170 175 180 aga tct ttc ggc ttt gcg gct tea ccg ttg ttg tat ctg cag gtg agt 691 Arg Ser Phe Gly Phe Wing Wing Pro Leu Leu Tyr Leu Gln Val Ser 185 190 195 ctt cgg cgg ggg tgg gtg aaa act cag att aag cca ccg aaa att acc gtg 739 Leu Arg Wing Trp Val Lys Thr Gln He Lys Pro Pro Lys He Thr Val i «* ,. - and - »? ~? Jat ^ -fc < «A ~ ^, 200 205 210 gag gcg gtg gac age aaa ggg cac aaa ttg caa aaa gag gaa gtg cca 787 Glu Ala Val Asp Ser Lys Gly His Lys Leu Gln Lys Glu Glu Val Pro 215 220 225 atg ctg ctt gcc tcg aat acc aat ccg tgg act tttg gt ctg ctt 835 Met Leu Leu Wing As As Thr Asn Pro Trp Thr Phe Leu Gly Pro Leu 230 235 240 245 ect gtg gtg aca aat ccg cag aat tct ttt gac aca ggt ctg ggg ctt 883 Pro Val Val Thr Asn Pro Gln Asn Ser Phe Asp Thr Gly Leu Gly Leu 250 255 260 ttt ggc ttg act agt gtg cga gga ttc ggg gga gtg gca gcg atg atg 931 Phe Gly Leu Thr Ser Val Arg Gly Phe Gly Val Val Ala Ala Met Met 265 270 - 275 cac ctg att ggc gtg ggg cat ggt cgg aag ttg gag aag ttg atc gct 979 His Leu He Gly Val Gly His Gly Arg Lys Leu Glu Lys Leu He Ala 10 280 285 290 aag cgc acc att gct ttt gat gat gcg gag aaa gta acg ctc acg tgc 1027 Lys Arg Thr He Wing Phe Asp Asp Wing Glu Lys Val Thr Leu Thr Cys 295 300 305 gac age gat cag cgt ttc caa gtt gat ggt gag tat gaa ggc aaa cca 1075 Asp Ser Asp Gln Arg Phe Gln Val Asp Gly Glu Glu Tyr Glu Gly Lys Pro 310 315 320 325 * here a gtg gtg ttg gaa tea atc act gat gcg gga gga gtg tat gcg 1123 Thr Lys Val Val Leu Glu Ser He Thr Asp Ala Val Arg Val Tyr Ala 15 330 335 340 ccg aag acg cat ccg aca ccg ccg atc atg aat tgg gct gtc cat ttg 1171 Pro Lys Thr His Pro Thr Pro Pro He Met Asn Ttp Ala Val His Leu 345 350 355 ttt aag cac gtc cgt gat ttc ctc cgg cgc cgc acg ttt ggc atc 1216 Phe Lys His Val Arg Asp Phe Leu Arg Val Arg Thr Phe Gly He 360 365 370 taggattcat cggagttttc ttc 1239 20 < 210 > 264 < 211 > 372 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 264 Met Thr Leu Lys Thr Ser Val Leu Ala Leu Leu Leu Asp Asn Val His 1 5 10 15 Val Leu Leu He Wing Asn Pro Glu Ser Thr Thr Gln Thr Gln Lys Leu 20 25 '30 25 Phe Arg Arg Val Val Pro Ala Leu Met Ala Leu Asp Gly Val Ser Leu 35 40 45 Glu Wing Arg Phe Thr His Tyr Gly Gly His Wing Glu Glu Met Val Wing 50 55 60 Gly Leu Thr Val Asp Asp Phe Asp Val He He Pro Wing Gly Gly Asp 65 70 75 80 Gly Thr Val Asn Glu Val He Asn Gly Leu Leu Gly Ser Wing Glu Gly 85 90 95 Asp Phe Arg Asn Leu Glu Asp Leu Pro Wing Wing Val Leu Pro Thr 100 105 110 Gly Ser Wing Asn Val Phe Wing Arg Wing Leu Gly Tyr Pro Thr Asp Pro 115 120 125 Tyr Wing Wing Wing Asp Wing Leu Val Glu Leu He Arg Lys Asn His Thr 130 135 140 Arg Thr He Thr Leu Gly Thr Trp Lys Gly Asp Asp Gln Gly Thr Arg 145 150 155 160 Trp Phe Wing Val Asn Wing Gly Phe Gly He Asp Wing Asp Val He Wing 165 170 175 Arg Val Glu Arg Ala Arg Ser Phe Gly Phe Wing Wing Pro Pro Leu Leu 180 185 190 Tyr Leu Gln Val Ser Leu Arg Wing Trp Val Lys Thr Gln He Lys Pro 195 200. 205 Pro Lys He Thr Val Glu Ala Val Asp Ser Lys Gly His Lys Leu Gln 210 215 220 Lys Glu Glu Val Pro Met Leu Leu Wing Ser Asn Thr Asn Pro Trp Thr 225 230 235 240 Phe Leu Gly Pro Leu Pro Val Val Thr Asn Pro Gln Asn Ser Phe Asp 245 250 255 Thr Gly Leu Gly Leu Phe Gly Leu Thr Ser Val Arg Gly Phe Gly Gly 260 265 270 Val Wing Wing Met Met His Leu He Gly Val Gly His Gly Arg Lys Leu 275 280 '285 Glu Lys Leu He Wing Lys Arg Thr He Wing Phe Asp Asp Ala Glu Lys 290 295 300 Val Thr Leu Thr Cys Asp Ser Asp Gln Arg Phe Gln Val Asp Gly Glu 305 310 315 320 Tyr Glu Gly Lys Pro Thr Lys Val Val Leu Glu Ser He Thr Asp Ala 325 330 335 Val Arg Val Tyr Ala Pro Lys Thr His Pro Thr Pro Pro He Met Asn 340 345 350 Trp Wing Val His Leu Phe Lys His Val Arg Asp Phe Leu Arg Val Arg 355 360 365 Thr Phe Gly He 370 < 210 > 265 < 211 > 271 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (271) < 223 > RXN03125 10 < 400 > 265 tgacaccggc gcgacgtatg gcattactgg cgtcacccca atttacgatg acatctctgc 60 tcgcctcggc gacgtcctgg ttccttacgt tctgatcgtt ttg gtt cta gcg ttc 115 • Leu Val Leu Ala Phe '1 5 ctc gtg ctg ttg ctc gtg ttc cgg tcc att tgg gtc cca ttg atc gcg 163 Leu Val Leu Leu Leu Val Phe Arg Ser He Trp Val Pro Leu He Wing 10 15 20 gct ctg ggc ttt ggc ttg tea gtt ctg gct acc ttt ggt gct acc gtg 211 15 Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr Phe Gly Wing Thr Val 25 30 35 gcg atc ttc caa gaa ggt gct tcc ggc atc atc gac gat ect cag cca 259 Wing He Phe Gln Glu Gly Wing Phe Gly He He Asp Asp Pro Gln Pro 40 45 '50 ctg ctg tgc ttc 271 Leu Leu Cys Phe 55 20 < 210 > 266 < 211 > 57 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 266 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser He Trp 1 5 10 15 Val Pro Leu He Wing Wing Leu Gly Phe Gly Leu Ser Val Leu Wing Thr 20 25 '' 30 25 Phe Gly Wing Thr Val Wing He Phe Gln Glu Gly Wing Phe Gly He He 35 40 45 Asp Asp Pro Gln Pro Leu Leu Cys Phe 50 55 < 210 > 267 < 211 > 1443 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1420) < 223 > RXN01553 < 400 > 267 atgatgatgt cctcagcaag tccaagcgcc aagecatget ggaaacaatt ctcgagctga 60 taccaagcca gacttaaatt tetacettaa agtcttgagc atg act gtt cag gaa 115 Met Thr Val Gln Glu 1 5 ttc gac cgc gcg acc aaa ccc aca cca aaa ccc qca att gtt tct tgg 163 Phe Asp Arg Ala Thr Lys Pro Thr Pro Lys Pro Pro He Val Ser Trp 10 15 20 gcg ttt tgg tgg ggg tcc gcc tct tcc aac gcg gtc ctc gtg acc 211 Wing Phe Trp Asp Trp Gly Ser Wing Being Phe Asn Wing Val Leu Val Thr 25 30 35 ttt att ttc tcg gtc tat ctc act gat tea gtc ggc gcc acc ctc ccc 259 Phe He Phe Ser Val Tyr Leu Thr Asp Ser Val Gly Wing Thr Leu Pro 40 45 50 gag ggt tcc aac gcc aca tea ctg tat tcg atg gcg gtc gcc atc gct 307 Glu Gly Ser Asn Ala Thr Ser Leu Tyr Ser Met Wing Val Wing Wing 55 60 .65 ggc gtc att gtt gcg gtt gtt gcc cca gtc atg ggc agg cga tea gat 355 Gly Val He Val Val Val Val Wing Pro Val Met Gly Arg Arg Ser Asp 70 75 80 85 atc aag ggc act cgc cgc agg tea ctg cgc atg tgg aca ctt gtc acc 403 He Lys Gly Thr Arg Arg Arg Ser Leu Arg Met Trp Thr Leu Val Thr 90 95 100 gtg ttc ttg atg ttt tgt ctc ttt aca gta aag aac act gat ccc aca 451 Val Phe Leu Met Phe Cys Leu Phe Thr Val Lys Asn Thr Asp Pro Thr 105 110 115 ttt ttc tgg ttt ggt gta gcc gtc atc atc gcg atc gcc aac atc accttt 499 Phe Phe Trp Phe Gly Val Wing Ala Met He Wing Asn He Thr Phe 120 125 130 gag ttc gct gaa gtt cag tac tat gcg cag ctc tcc caa atc tcg acc 547 Glu Phe Wing Glu Val Gln Tyr Tyr Wing Gln Leu Ser Gln He Ser Thr 135 140 145. cgc gaa aac gtg ggc cga gtt tct ggt ttc ggc tgg tcc atg ggt tac 595 Arg Glu Asn Val Gly Arg Val Ser Gly Phe Gly Trp Ser Met Gly Tyr 150 155 160 165 ttc ggt ggc atc gtt cta ctg ctt gtt tgt tac cta ggt ttt gtt gcc 643 Phe Gly Gly He Val Leu Leu Leu Val Cys Tyr Leu Gly Phe Val Wing 170 175 180 ggt gat ggc gat acc cgc gga ttc cta aac ctg ccc atc gaa gac ggc 691 Gly Asp Gly Asp Thr Arg Gly Phe Leu Asn Leu Pro He Glu Asp Gly 185 190 195 atg aat atc cgc ctc gtc gca gtg ctt gca gcc gtt tgg ttc ttg gtc 739 Met Asn He Arg Leu Val Wing Val Leu Wing Wing Val Trp Phe Leu Val 200 205 210 tct gcg att ccg gca ctt ctt cga gtc cca gaa att gag gca cag gta 787 Be Ala He Pro Ala Leu Leu Arg Val Pro Glu He Glu Ala Gln Val 215 220 225 gct gcc gaa gac cac ccc aaa ggc ctc ata gct gcc tac aag gat ctc 835 Ala Ala Glu Asp His Pro Lys Gly Leu He Wing Wing Tyr Lys Asp Leu 230 235 240 245 ttt ggg cag atc gct gag ctg tgg aaa caa gac cgc aac tcc gtg tat 883 Phe Gly Gln He Wing Glu Leu Trp Lys Gin Asp Arg Asn Ser Val Tyr 250 255 260 ttc ctc atc gca gca act gtt ttc cgt gac gga ctc gcc gga gta ttt 931 Phe Leu He Ala Wing Thr Val Phe Arg Asp Gly Leu Wing Gly Val Phe 265 270 275 acc ttc ggt gcc atc ctt gcg gtc tct gtg tac gga cta tct gcc ggt 979 Thr Phe Gly Ala He Leu Ala Val Ser Val Tyr Gly Leu Ser Ala Gly 280 285 290 gat gtc ctc ctc ttc ggt gtc gca gcc aac gtg gtc tct gcg ttg gga 1027 Asp Val Leu Leu Phe Gly Val Ala Wing Asn Val Val Ser Wing Leu Gly 295 300 305 gca ctc ctc gga gga ttc cta gac gat cgc gtc ggg cca aaa ccc atc 1075 Wing Leu Leu Gly Gly Phe Leu Asp Asp Arg Val Gly Pro Lys Pro He 310 315 320 325 atc ttg att tct ctt gcc atc atc atc gcc gat gct gca att ctc ttc 1123 He Leu He Ser Leu Ala He Met He Wing Asp Ala. Ala He Leu Phe 330 335 340 ttc gtt gaa ggc ccc act aat ttc tgg atc ttc gga tta atc ctc tgt 1171 Phe Val Glu Gly Pro Thr Asn Phe Trp He Phe Gly Leu He Leu Cys 345 350 355 gcg ttt gtg gga ect gca cag tea gcg tcg aga age tat tta aca cgt 1219 Wing Phe Val Gly Pro Wing Gln Ser Wing Being Arg Ser Tyr Leu Thr Arg 360 365 370 ctt tcc cca gat ggc cag gaa ggc cag ctc ttc ggc ctt tat gcc act 1267 Leu Ser Pro Asp Gly Gln Glu Gly Gln Leu Phe Gly Leu Tyr Wing Thr 375 380 385 acc ggc cgt gcc gtg agt tgg atg gtg ccg tcg ctg ttt ggt gta ttt 1315 Thr Gly Arg Wing Val Ser Trp Met Val Pro Ser Leu Phe Gly Val Phe 390 395 400 405 gtg ggg ctc acc ggc gat gac cgc act ggt att ttg gcc atc gcg ctg 1363 Val Gly Leu Thr Gly Asp Asp Arg Thr Gly He Leu Wing He Wing Leu 410 415 420 att ctg cta ttc ggt att gtg ctg ctg age atg gtg aag cca ccg cac 1411 He Leu Leu Phe Gly He Val Leu Leu Ser Met Val Lys Pro Pro His 425 430 435 aag gtg aag tagacaaage gcccacaagg att 1443 Lys Val Lys 440 < 210 > 268 < 211 > 440 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 268 Met Thr Val Gln Glu Phe Asp Arg Wing Thr Lys Pro Thr Pro Lys Pro 1 5 10 15 Pro He Val Ser Trp Wing Phe Trp Asp Trp Gly Ser Wing Ser Phe Asn 20 25 30 Wing Val Leu Val Thr Phe He Phe Ser Val Tyr Leu Thr Asp Ser Val 35 40 45 Gly Wing Thr Leu Pro Glu Gly Ser Asn Wing Thr Ser Leu Tyr Ser Met 50 55 60 Wing Val Wing Wing Wing Gly Val He Val Val Wing Val Val Wing Pro Val Met 65 70 75 80 Gly Arg Arg Ser Asp He Lys Gly Thr Arg Arg Arg Ser Leu Arg Met 85 90 95 Trp Thr Leu Val Thr Val Phe Leu Met Phe Cys Leu Phe Thr Val Lys 100 105 110 Asn Thr Asp Pro Thr Phe Phe Trp Phe Gly Val Wing He Met Wing He 115 120 125 Wing Asn He Thr Phe Glu Phe Wing Glu Val Gln Tyr Tyr Wing Gln Leu 130 135 '140 Ser Gln He Ser Thr Arg Glu Asn Val Gly Arg Val Ser Gly Phe Gly 145 150 155 160 U ^^^ MB ^ M Trp Ser Met Gly Tyr Phe Gly Gly He Val Leu Leu Leu Val Cys Tyr 165 170 175 Leu Gly Phe Val Wing Gly Asp Gly Asp Thr Arg Gly Phe Leu Asn Leu 180 185 190 Pro He Glu Asp Gly Met Asn He Arg Leu Val Wing Val Leu Wing Wing 195 200 205 Val Trp Phe Leu Val Ser Wing Pro Pro Wing Leu Leu Arg Val Pro Glu 210 215 220 He Glu Wing Gln Val Wing Wing Glu Asp His Pro Lys Gly Leu He Wing 225 230 235 240 Wing Tyr Lys Asp Leu Phe Gly Gln He Wing Wing Glu Leu Trp Lys Gln Asp 245 250 255 Arg Asn Ser Val Tyr Phe Leu He Wing Wing Thr Val Phe Arg Asp Gly 260 265 270 Leu Wing Gly Val Phe Thr Phe Gly Wing He Leu Ala Val Ser Val Tyr 275 280 285 Gly Leu Ser Ala Gly Asp Val Leu Leu Phe Gly Val Ala Ala Asn Val 290 295 300 Val Ser Ala Leu Gly Ala Leu Leu Gly Gly Phe Leu Asp Asp Arg Val 305 310 315 320 Gly Pro Lys Pro He He Leu He Ser Leu Wing He Met He Wing Asp 325 330 335 Wing Wing He Leu Phe Phe Val Glu Gly Pro Thr Asn Phe Trp He Phe 340 345 350 Gly Leu He Leu Cys Wing Phe Val Gly Pro Wing Gln Ser Ala Ser Arg 355 360 365 Ser Tyr Leu Thr Arg Leu Ser Pro Asp Gly Gln Glu Gly Gln Leu Phe 370 375 380 Gly Leu Tyr Wing Thr Thr Gly Arg Wing Val Ser Trp Met Val Pro Ser 385 390 395 400 Leu Phe Gly Val Phe Val Gly Leu Thr Gly Asp Asp Arg Thr Gly He 405 410 415 Leu Ala He Ala Leu He Leu Leu Phe Gly He Val Leu Leu Ser Met 420 425 430 Val Lys Pro Pro His Lys Val Lys 435 440 < 210 > 269 i ^ .. -,. . ^ ...,, -v ^ ....... . . . . . "., ,,. . ..., < 211 > 840 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (817) < 223 > RXN00535 < 400 > 269 aatcgcatgg ggcaccgtgg tcagacaccg gatcgcgctc c.gcaccccaa aagatggctc 60 cctaaggagc tcacctttac tcaatgctct gatgacaccg atg. tgg tgg gg ggc 115 Met Trp Trp Wing Gly 1 5 atg agt acc gcg atg ctg gca tat ttc tta cata aca gta gca ctt ggt 163 Met Ser Thr Ala Met Leu Ala Tyr Phe Leu Gln Thr Val Ala Leu Gly 10 15 20 10 ttc ggc acc ctc ttg gta gtg caa cca gtg ctt gtc ctg tcg ctg atg 211 Phe Gly Thr Leu Leu Val Val Gln Pro Val Leu Val Leu Ser Leu Met 25 30 35 ttc acg ctg ccg ctc tea gca cga ttc aat ggc tac cga cta cta cgc cga 259 Phe Thr Leu Pro Leu Ser Wing Arg Phe Asn Gly Tyr Arg Leu Arg Arg 40 45 50 act gaa atc ttc tgg gct acc ctc ctc acc gta gcg ggc atc atg 307 Thr Glu He Phe Trp Wing Thr Leu Leu Thr Val Wing Val Gly He Met 55 60 65 15 atc gtt ttg gga cgc ccc ctt ccc gga aac ccc cac ccc cca ctc gat 355 He Val Leu Gly Arg Pro Leu Pro Gly Asn Pro His Pro Pro Leu Asp 70 75 80 - 85 cga tgg att cca gta ctt tta gtc ggc gtt gca gta atg ggt gga atg 403 Arg Trp He Pro Val Leu Leu Val Gly Val Ala Val Met Gly Gly Met 90 95 100 tgg ctg ctt gcg gaa tac gta tta aag aag gac aaa gcc ctc atc ctt 451 Trp Leu Leu Wing Glu Tyr Val Leu Lys Lys Asp Lys Wing Leu He Leu 105 110 115 20 ggt ctt gtg acg ggt gca ttg ttt ggc tac gta gta gtg atg tcc aaa 499 Gly Leu Val Thr Gly Wing Leu Phe Gly Tyr Val Wing Val Met Ser Lys 120 125 130 gcc gcg gtg gat ctt ttt gtc cat caa ggc ata acg gga ctc atc ttg 547 Ala Ala Val Asp Leu Phe Val His Gln Gly He Thr Gly Leu He Leu 135 140 145 aac tgg gaa ggc tac ggc cta atc ctc acc gca tta ctt gga aca atc 595 Asn Trp Glu Gly Tyr Gly Leu He Leu Thr Ala Leu Leu Gly Thr He 150 155 160 165 25 gtg cag tat tcc ttt aac gct ggc gaa cta caa aaa tcg cta ccc 643 í «^ ^ jg £ gg ^ gj | g | j Val Gln Gln Tyr Ser Phe Asn Wing Gly Glu Leu Gln Lys Ser Leu Pro 170 175 180 gcc atg acc att gcc gaa cca att gtt gcc ttc agt ttg ggc tac ttg 691 Wing Met Thr He Wing Glu Pro He Val Wing Phe Ser Leu Gly Tyr Leu 185 190 195 gtt ctg ggc gaa aaa ttc caa gtc gtg gao tgg gaa tgg atc gcc atg 739 Val Leu Gly Glu Lys Phe Gln Val Val Asp Trp Glu Trp He Ala Met 200 205 210 ggc atc gca cta ctg gtg atg att gtt tcc action att gca ctg tct cgt 787 Gly He Ala Leu Leu Val Met He Val Ser Thr He Ala Leu Ser Arg 215 220 225 'aca age aca atg ccg gcc gga tcg aaa agg taaaactcca aagttccccc 837 Thr Ser Thr Met Pro Ala Gly Ser Lys Arg 230 235 cga 840 10 < 210 > 270 < 211 > 239 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 270 Met Trp Trp Wing Gly Met Ser Thr Wing Met Leu Wing Tyr Phe Leu Gln 1 5 10 15 Thr Val Wing Leu Gly Phe Gly Thr Leu Leu Val Val Gln Pro Val Leu 15 20 25 30 Val Leu Ser Leu Met Phe Thr Leu Pro Leu Ser Wing Arg Phe Asn Gly 35 40 45 Tyr Arg Leu Arg Thr Glu He Phe Trp Wing Thr Leu Leu Thr Val 50 55 60 Wing Val Gly He Met He Val Leu Gly Arg Pro Leu Pro Gly Asn Pro 65 70 75 80 His Pro Pro Leu Asp Arg Trp He Pro Val Leu Leu Val Gly Val Ala 20 85 90 95 Val Met Gly Gly Met Trp Leu Leu Wing Glu Tyr Val Leu Lys Lys Asp 100 105 110 Lys Ala Leu He Leu Gly Leu Val Thr Gly Ala Leu Phe Gly Tyr Val 115 120 125 Wing Val Met Ser Lys Wing Wing Val Asp Leu Phe. Val His Gln Gly He 130 135 140 Thr Gly Leu He Leu Asn Trp Glu Gly Tyr Gly Leu He Leu Thr Wing 25 145 150 155 160 Leu Leu Gly Thr He Gl Gln Gln Tyr Ser Phe Asn Wing Gly Glu Leu 165 170 175 Gln Lys Ser Leu Pro Wing Met Thr He Wing Glu Pro He Val Wing Phe 180 185 190 Ser Leu Gly Tyr Leu Val Leu Gly Glu Lys Phe Gln Val Val Asp Trp 195 200 205 Glu Trp He Wing Met Gly He Wing Leu Leu Val M, and He Val Ser Thr 210 215 220 Wing Wing Leu Ser Arg Thr Ser Thr Met Pro Wing Gly Ser Lys Arg 225 230 235 < 210 > 271 < 211 > 2472 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (2449) < 223 > RXN00453 < 400 > 271 tagtggggcg tgaaaaaata gctcatttaa gaggagaagc aaccccgtgg cgaaattgct 60 attcaggttg gggcgatggt cctataatcg caagtggatt gtg att tcg gca tgg 115 Val He Ser Wing Trp 1 5 cta ctt att ttg gcc att gtt ggt cgt ctg gcc ctg acg atg cag aag 163 Leu Leu He Leu Ala He Val Gly Gly Leu Wing Leu Thr Met Gln Lys 10 15 '20 ggg ttc agt aac tct tct act att gaa gac acc ect tcg att gat gcc 211 Gly Phe Ser Asn Ser Phe Thr He Glu Asp Thr Pro Ser He Asp Wing 25 30 35 act gtt tct ctg gtt gaa aat ttc ect gat cag acg aac ccg gtg acg 259 Thr Val Ser Leu Val Glu Asn Phe Pro Asp Gln Thr Asn Pro Val Thr 40 45 50 gcc g gt g gt aac gtg gtt ttc caa tcc ccg gaa gga acc acg ctt 307 Wing Wing Gly Val Val Val Phen Gln Ser Pro Glu Gly Thr Thr Leu 55 60 65 gat gat ect cag atg atg act gcg at g gat gtc gtt gat tac att 355 Asp Asp Pro Gln Met Met Thr Ala Met Asp Ala Val Val Asp Tyr He 70 75 80 85 gag gac aat ttg ect gat ttt ggt ggg gga gag cgc ttc ggc aat ect 403 Glu Asp Asn Leu Pro Asp Phe Gly Gly Gly Glu Arg Phe Gly Asn Pro 90 95 100 gtt gag gtg tct ect gcg ttg gaa gag atg gtc atc gag cag atg acc 451 Val Glu Val Ser Pro Ala Leu Glu Glu Met Val He Glu Gln Met Thr 105 110 115 age atg ggg ctt ect gag gaa acc gct gca aag gat gct gcc aat ctg 499 Ser Met Gly Leu Pro Glu Glu Thr Wing Wing Lys Asp Wing Wing Asn Leu 120 125 130 gcg gtg ttg age gaa gac aaa acc att ggc tac acc tct ttc aac att 547 Wing Val Leu Ser Glu Asp Lys Thr He Gly Tyr Thr Ser Phe Asn He 135 140 145 gat gtt gag gcc gca gaa tat gtg gag caa aaa cac cgc gat gtg atc 595 Asp Val Glu Wing Wing Glu Tyr Val Glu Gln Lys His Arg Asp Val He 150 155 160 165 aac gaa gcg atg caa atc ggt gaa gat tta ggt gtc cgg gtg gaa gcc 643 Asn Glu Wing Met Gln He Gly Glu Asp Leu Gly Val Arg Val Glu Wing 170 175 180 ggt gga ect gct ttc ggt gat cca att cag att gaa acc acc agt gag 691 Gly Gly Pro Wing Phe Gly Asp Pro He Gln He Glu Thr Thr Ser Glu 185 190 195 atc atc ggt att ggc atc gcg ttc atc gtg ttg att ttc acc ttt ggt 739 He He Gly He Gly He Wing Phe He Val Leu He Phe Thr Phe Gly 200 205 210 tct ttg att gct gca ggc ttg ect ttg att acc gcg gtg atc ggc gtg 787 Be Leu He Wing Wing Gly Leu Pro Leu He Thr Wing Val He Gly Val 215 220 225 ggc att ggt gcg ctg gca att gtg ctg gcc acg gcg ttt act gat ctc 835 Gly He Gly Ala Leu Ala He Val Leu Ala Thr Ala Phe Thr Asp Leu 230 235 240 245 aac aat gtg act cca gtg ctc gca gtg atg att ggc ctg gcc gtg ggc 883 Asn Asn Val Thr Pro Val Leu Ala Val Met He Gly Leu Ala Val Gly 250 255 260 att gac tac gcg ctg ttt att ttg tct agg tac cgt gcg gag tat aag 931 He Asp Tyr Ala Leu Phe He Leu Ser Arg Tyr Arg Wing Glu Tyr Lys 265 270 275 cgc atg cca cgt gcc gcc gat gct gga atg gcg gtg ggc aca gct ggt 979 Arg Met Pro Arg Wing Asp Wing Wing Gly Met Wing Val Gly Thr Wing Gly 280 285 290 agt gcg gtg gtg ttt gct ggc gcg acg gtg att atc gcg ctg gta gcc 1027 Ser Ala Val Val Phe Ala Gly Ala Thr Val He He Ala Leu Va l Wing 295 300 305 ctc atc att gcg gat atc gga ttc ctc acg gcc atg ggt att tct gcg 1075 Leu He He Wing Asp He Gly Phe Leu Thr Wing Met Gly He Ser Wing 310 315 320 325 gcg ttt acg gtg ttc gtg gct gtg ctc att gcg ttg acg ttt atc ccg 1123 Wing Phe Thr Val Phe Val Wing Val Leu He Wing Leu Thr Phe He Pro 330 335 340 gcg ctg ttg ggt gtg ttt ggt ggt cat gcg ttc aag ggc aag atc ect 1171 Wing Leu Leu Gly Val Phe Gly Gly His Wing Phe Lys Gly Lys He Pro 345 350 355 gga att ggt gga aac cca acg cca aag cag acg tgg gag caa gcg ctt 1219 Gly He Gly Gly Asn Pro Thr Pro Lys Gln Thr Trp Glu Gln Ala Leu 360 365 370 aat cgt cgt tcc aag ggt cgc tea tgg gtc aag ctt gta cag aaa gca 1267 Asn Arg Arg Ser Lys Gly Arg Ser Trp Val Lys Leu Val Gln Lys Wing 375 380 385 ccg ggt ctt gtg gtg gca gtg gtg gtc ttg ggt ctt ggt gcc ttg acc 1315 Pro Gly Leu Val Val Ala Val Val Leu Gly Leu Gly Ala Leu Thr 390 395 400 405 att ect gca atg aac ctg cag ttg tea ctctctct tct gacccccccc 1363 He Pro Ala Met Asn Leu Gln Leu Se r Leu Pro Ser Asp Ser Thr Ser 410 415 420 aat att gat ac act cgt cag tcg gct gat ttg atg gca gag ggc 1411 Asn He Asp Thr Thr Gln Arg Gln Ser Wing Asp Leu Met Wing Glu Gly 425 430 435 ttt ggc gcg ggc gtt aat gcg gcg ccg ttc ttg gtc atc gtc gat acg cat 1459 Phe Gly Wing Gly Val Asn Wing Pro Phe Leu Val He Val Asp Thr His 440 445 450 gag gtc gtc aat gct gat tcc acc gca ttg cag cca ctg att gag gca cag 1507 Glu Val Asn Wing Asp Ser Thr Wing Leu Gln Pro Leu He Glu Wing Gln 455 460 4.65 gag ect gaa gag ggc gag ttc gat cgg gag cag gcg gct cgt ttt gct 1555 Glu Pro Glu Glu Gly Glu Glu Phe Asp Arg Glu Gln Ala Ala Arg Phe Wing 470 475 480 485 acc tat atg tat gtc acc acc aac tac aac atc gat gtg aag 1603 Thr Tyr Met Tyr Val Thr Gln Thr Tyr Asn Ser Asn He Asp Val Lys 490 495 500 aat gcg cag atc atc age gtc aat gat gat ttc act gcg gcg cag att 1651 Asn Wing Gln He He Ser Val Asn Asp Asp Phe Thr Wing Wing Gln He 505 510 515 ctc gtg act cca tac acc gga ect gcg gat aaa gag acc ect gag ttg 1699 Leu Val Thr Pro Tyr Thr Gly Pro Wing Asp Lys Glu Thr Pro Glu Leu 520 525 530 atg cac gtg ctg cgt gcg cag gaa gct cag att gag gat gtt acg gga 1747 Met His Val Leu Arg Ala Gln Glu Ala Gln He Glu Asp Val Thr Gly 535 540 545 act gaa ctg ggt acc act ggg ttt acg gcg gtt cag ttg gac att act 1795 "• * • * -» - '- • * • - * - • •• ~ - - Thr Glu Leu Gly Thr Thr Gly Phe Thr Wing Val Gln Leu Asp He Thr 550 555 560 565 gag cag ctg gaa gac gca atg ccg gtt tac ctc gct gtg gtt gtt ggt 1843 Glu Gln Leu Glu Asp Wing Met Pro Val Tyr Leu Wing Val Val Val Gly 570 575 580 ttg gct att ttc ctc ctc att ctg gtg ttc cgt tcc ctg ctt gtt ccg 1891 Leu Ala He Phe Leu Leu He Leu Val Phe Arg Ser Leu Leu Val Pro 585 590 595 ctg gtt gct ggc ctt ggc ttc ttg ttg tct gtg ggt gcg gcc gt ttc 1939 Leu Val Wing Gly Leu Gly Phe Leu Leu Ser Val Gly Wing Wing Phe Gly 600 605 610 gcg acg gtg ttg gtc tgg cag gag ggc ttc ggt ggc ttt gtg aac acc 1987 Wing Thr Val Leu Val Trp Gln Glu Gly Phe Gly Gly Phe Val Asn Thr 615 620 625 ect ggt ccg ctg att tcc ttc atg ccg atc ttc ctc atc ggc gtg acc 2035 10 Pro Gly Pro Leu He Be Phe Met Pro He Phe Leu He Gly Val Thr 630 635 640 645 ttc ggt ttg gcc atg gac tat cag gtg ttc ctt gtg act cgc atg cgc 2083 • Phe Gly Leu Wing Met Asp Tyr Gln Val Phe Leu Val Thr Arg Met Arg 650 655 660 gag cac tac acc cac cac aat ggc aag gga cag ect ggt tcc aag tac 2131 Glu His Tyr Thr His His Asn Gly Lys Gly Gln Pro Gly Ser Lys Tyr 665 6 ^ 0 675 acc ccg gtt gag cag tea gtg att gaa ggc ttc acg cag ggc tcc cgc 2179 15 Thr Pro Val Glu Gln Ser Val He Glu Gly Phe Thr Gln Gly Ser Arg 680 685 690 gtg gtt aca gca gcg gca ctg atc atg att gcc gtg ttc gtg gcg ttt 2227 Val Val Thr Ala Ala Ala Leu He Met He Ala Val Phe Val Ala Phe 695 700 705 att gat cag ccg ttg cca ttt att aag atc ttc ggt tcc gcg ttg ggt 2275 He Asp Gln Pro Leu Pro Phe He Lys He Phe Gly Phe Ala Leu Gly • 710 715 720 725 gcg ggc gtg ttt ttc gat gct ttc ttc att cgc atg ggt ctg gtc ccc 2323 20 Wing Gly Val Phe Phe Asp Wing Phe Phe He Arg Met Gly Leu Val Pro 730 735 740 gcg tcg atg ttc ctg atg ggc aag gcc acg tgg atgg ect aag tgg 2371 Wing Ser Met Phe Leu Met Gly Lys Wing Thr Trp Trp Met Pro Lys Trp 745 750 755 ctg gat cga att ctg cca agt ttg gac att gaa ggc acc gca ctg gag 2419 Leu Asp Arg He Leu Pro Ser Leu Asp He Glu Gly Thr Ala Leu Glu 760 765 770 aag gaa tgg gag gag aag cag gct gca cgt tagacttggc acctatgtca 2469 25 Lys Glu Trp Glu Glu Lys Gln Wing Ala Arg 775 780 gat 2472 < 210 > 272 < 211 > 783 < 212 > PRT < 213 > Corynebacterium gluta icum < 400 > 272 Val He Ser Wing Trp Leu Leu He Leu Wing He Val Gly Gly Leu Wing 1 5 10 15 Leu Thr Met Gln Lys Gly Phe Ser Asn Ser Phe Thr He Glu Asp Thr 20 25 30 Pro Ser He Asp Ala Thr Val Ser Leu Val Glu Asn Phe Pro Asp Gln 35 40 45 10 Thr Asn Pro Val Thr Ala Ala Gly Val Asn Val Val Phe Gln Ser Pro 50 55 60 Glu Gly Thr Thr Leu Asp Asp Pro Gln Met Met Thr Ala Met Asp Ala 65 70 75 80 Val Val Asp Tyr He Glu Asp Asn Leu Pro Asp Phe Gly Gly Gly Glu 85 90 95 Arg Phe Gly Asn Pro Val Glu Val Ser Pro Ala Leu Glu Glu Met Val 100 105 110 15 He Glu Gln Met Thr Ser Met Gly Leu Pro Glu Glu Thr Ala Ala Lys 115 120 125 Asp Ala Wing Asn Leu Wing Val Leu Ser Glu Asp Lys Thr He Gly Tyr 130 135 140 Thr Ser Phe Asn He Asp Val Glu Wing Wing Glu Tyr Val Glu Gln Lys 145 150 155 160 His Arg Asp Val He Asn Glu Ala Met Gln He Gly Glu Asp Leu Gly 165 170 175 20 Val Arg Val Glu Wing Gly Gly Pro Wing Phe Gly Asp Pro He Gln He 180 185 190 Glu Thr Ser Glu He He Gly He Gly He Wing Phe He Val Leu 195 200 205 He Phe Thr Phe Gly Ser Leu He Wing Ala Gly Leu Pro Leu He Thr 210 215 220 Wing Val He Gly Val Gly He Gly Wing Leu Wing He Val Leu Wing Thr 225 230 235 240 25 Wing Phe Thr Asp Leu Asn Asn Val Thr Pro Val Leu Val Val Met He 245 250 255 Gly Leu Ala Val Gly He Asp Tyr Ala Leu Phe He Leu Ser Arg Tyr 260 265 270 Arg Ala Glu Tyr Lys Arg Met Pro Arg Ala Asp Ala Ala Gly Met Ala 275 280 285 Val Gly Thr Ala Gly Ser Ala Val Val Phe Ala Gly Ala Thr Val He 290 295 300 He Ala Leu Val Ala Leu He He Ala Asp He Gly Phe Leu Thr Ala 305 310 315 320 Met Gly He Ser Ala Ala Phe Thr Val Phe Val Ala Val Leu He Ala 325 330 335 Leu Thr Phe He Pro Wing Leu Leu Gly Val Phe Gly Gly His Wing Phe 340 345 350 Lys Gly Lys He Pro Gly He Gly Gly Asn Pro Thr Pro Lys Gln Thr 355 360 365 Trp Glu Gln Ala Leu Asn Arg Arg Ser Lys Gly Arg Ser Trp Val Lys 370 375 380 Leu Val Gln Lys Wing Pro Gly Leu Val Val Wing Val Val Val Leu Gly 385 390 395 400 Leu Gly Ala Leu Thr He Pro Ala Met Asn Leu Gln Leu Ser Leu Pro 405 410 415 Being Asp Ser Thr Ser Asn He Asp Thr Thr Gln Arg Gln Ser Wing Asp 420 425 430 Leu Met Wing Glu Gly Phe Gly Wing Gly Val Asn Wing Pro Phe Leu Val 435 440 445 He Val Asp Thr His Glu Val Asn Wing Asp Ser Thr Ala Leu Gln Pro 450 455 460 Leu He Glu Wing Gln Glu Pro Glu Glu Gly Glu Phe Asp Arg Glu Gln 465 470 475 480 Wing Wing Arg Phe Wing Thr Tyr Met Tyr Val Thr Gln Thr Tyr Asn Ser 485 490 495 Asn He Asp Val Lys Asn Wing Gln He He Ser Val Asn Asp Asp Phe 500 505 510 Thr Wing Wing Gln He Leu Val Thr Pro Tyr Thr Gly Pro Wing Asp Lys 515 520 525 Glu Thr Pro Glu Leu Met His Val Leu Arg Ala Gln Glu Ala Gln He 530 535 540 Glu Asp Val Thr Gly Thr Glu Leu Gly Thr Thr Gly Phe Thr Ala Val 545 550 555 560 Gln Leu Asp He Thr Glu Gln Leu Glu Asp Wing Met Pro Val Tyr Leu 565 570 575 Wing Val Val Val Gly Leu Wing He Phe Leu Leu He Leu Val Phe Arg 580 585 590 Ser Leu Leu Val Pro Leu Val Wing Gly Leu Gly Phe Leu Leu Ser Val 595 600 605 Gly Wing Wing Phe Gly Wing Thr Val Leu Val Trp Gln Glu Gly Phe Gly 610 615 620 Gly Phe Val Asn Thr Pro Gly Pro Leu He Ser Phe Met Pro He Phe 625 630 635 640 Leu He Gly Val Thr Phe Gly Leu Ala Met Asp Tyr Gln Val Phe Leu 645 650 655 Val Thr Arg Met Arg Glu His Tyr Thr His His Asn Gly Lys Gly Gln 660 665 670 Pro Gly Ser Lys Tyr Thr Pro Val Glu Gln Ser Val He Glu Gly Phe 675 680 685 Thr Gln Gly Ser Arg Val Val Thr Ala Ala Ala Leu He Met Wing 690 695 700 Val Phe Val Wing Phe He Asp Gln Pro Leu Pro Phe He Lys He Phe 705 710 715 720 Gly Phe Ala Leu Gly Wing Gly Val Phe Phe Asp Wing Phe Phe He Arg 725 730 735 Met Gly Leu Val Pro Wing Wing Met Phe Leu Met Gly Lys Wing Thr Trp 740 745 750 Trp Met Pro Lys Trp Leu Asp Arg He Leu Pro Ser Leu Asp He Glu 755 760 765 Gly Thr Ala Leu Glu Lys Glu Trp Glu Glu Lys Gln Ala Wing Arg 770 775 780 < 210 > 273 < 211 > 597 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (574) < 223 > RXN00932 < 400 > 273 cccaattaat ttatgcactt cggtgaggtt actcacaaag agtagcgtgc aaagcccagc 60 aataaggtga tgtttcaacg attaggttac ggtaggggcc atg acg cca cag aaa 115 Met Thr Pro Gln Lys 1 5 ctt cac cgt ttt gca gcc ctt tta gaa atg ggt acc tgg acc ctg ctg 163 Leu His Arg Phe Ala Ala Leu Leu Glu Met Gly Thr Trp Thr Leu Leu 10 15 20 • atc atc ggc atg atc tta aaa tac agt gga gtg aca gac gcc gta acc 211 He He Gly Met He Leu Lys Tyr Ser Gly Val Thr Asp Ala Val Thr 25 30 35 ect att gcc ggc ggt atc cac ggc ttt ggc ttc ctc tgt ttt gca gcc 259 Pro He Wing Gly Gly He His Gly Phe Gly Phe Leu Cys Phe Wing Wing 40 45 50 atc acc atc acc gtg tgg atc aat aat aat tgg aca ttc ccg cag ggt 307 He Thr He Thr Val Trp He Asn Asn Lys Trp Thr Phe Pro Gln Gly 55 60 65 10 atc gca ggt ttg atc gtc tct gtt atc ccg tgg gct gca ttg cca ttt 355 He Wing Gly Leu He Val Ser Val He Pro Trp Wing Wing Leu Pro Phe 70 75 80 85 gca ttg tgg gca gac aag aag ggc ctc gtt gcc ggc gga tgg cgc ttt 403 Wing Leu Trp Wing Asp Lys Lys Gly Leu Val Wing Gly Gly Trp Arg Phe 90 95 100 tea gat ccg tcc gaa aag cca cac act ttc gac aag atc ttg gct 451 Ser Asp Pro Ser Glu Lys Pro His Thr Phe Phe Asp Lys He Leu Ala 105 110 115 15 caa ttg gtc agg cac cca atc cga tcc att tta att ctg ctg gtg att 499 Gln Leu Val Arg His Pro He Arg Ser He Leu He Leu Leu Val He 120 125 130 atc gcc gtc gtc ttc tct atc ttg ctg gcg atg gga cca ect tat gat 547 He Ala Val Val Phe Ser He Leu Leu Wing Met Gly Pro Pro Tyr Asp 135 140 145 cca gat gcc atc gca aac act gtg gat taaacaacag cctccttcac 594 Pro Asp Ala He Ala Asn Thr Val Asp 150 155 20 atg 597 < 210 > 274 < 211 > 158 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 274 Met Thr Pro Gln Lys Leu His Arg Phe Ala Ala Leu Leu Glu Met Gly 1 5 10 15 25 - ^^^. » ... IV ....

Thr Trp Thr Leu Leu He He Gly Met He Leu Lys Tyr Ser Gly Val 20 25 30 Thr Asp Wing Val Thr Pro He Wing Gly Gly He His Gly Phe Gly Phe 35 40 45 Leu Cys Phe Wing Wing He Thr He Thr Val Trp He Asn Asn Lys Trp 50 55 60 Thr Phe Pro Gln Gly He Wing Gly Leu He Val Ser Val He Pro Trp 65 70 75 80 Ala Ala Leu Pro Phe Ala Leu Trp Ala Asp Lys Lys Gly Leu Val Wing 85 90 95 Gly Gly Trp Arg Phe Ser Asp Pro Ser Glu Lys Pro His Thr Phe Phe 100 105 110 Asp Lys He Leu Wing Gln Leu Val Arg His Pro He Arg Ser He Leu 115 120 125 10 He Leu Leu Val He He Wing Val Val Phe Ser He Leu Leu Wing Meo 130 135 140 Gly Pro Pro Tyr Asp Pro Asp Wing He Wing Asn Thr Val Asp 145 150 155 < 210 > 275 < 211 > 534 < 212 > DNA < 213 > Corynebacterium glutamicum 15 < 220 > < 221 > CDS < 222 > (65) .. (511) < 223 > RXN03022 < 400 > 275 acgcctgtgt catccttttc attagagtgg agaaaagccc atacagaaag ttggcgcccg 60 • agca gtg atc atc acc gct ggc atc ttg gta gcg acc gcg acc gcc ctc 109 Val He He Thr Ala Gly He Leu Val Ala Thr Ala Thr Ala Leu 1 5 10 15 20 cta atg atc acc gcg gtc age gag tea acg tac atc gtc atc tcc ctc 157 Leu Met He Thr Wing Val Ser Glu Ser Thr Tyr He Val He Ser Leu 20 25 30 gcc gc tc tc ctt tat ggc ctt gcc ctc gga ctc ctc tcc gcc acc 205 Gly Phe Ser Leu Tyr Gly Leu Gly Leu Gly Leu Phe Wing Thr Pro 35 40 45 gtc acc gat act gcg ctt gga aca ctt ccc aaa gac cgt acc ggc gct 253 Val Thr Asp Thr Ala Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Ala 50 55 60 25 - ^^ «a ^« '- ^ .- ... .. ... . . . ...... »**» > ggt gca ggt gta ttc aag atg tcc tct tcc ctc ggc gca gca ctc ggc 301 Gly Wing Gly Val Phe Lys Met Being Ser Leu Gly Ala Wing Leu Gly 65 70 75 atc gca atc tcc act tea gtg ttc ctc gca ctt cgc gac ggc acc tcc 349 He Wing He Ser Thr Ser Val Phe Leu Wing Leu Arg Asp Gly Thr Ser 80 85 90 95 atc aac tcc gac gtc gca ctc gcc gga gtt te gtt tea ctt gc atc aac 397 He Asn Ser Asp Val Ala Leu Wing Gly Thr Val Ser Leu Gly He Asn 100 105 110 gtt gta tc gca gca gca aca gcc acc atc gca gca gtc ctt att cca 445 Val Val Phe Ala Ala Thr Ala Thr He Thr Ala Ala Val Leu He Pro 115 120 125 aaa gcc gct ggc aaa gtc tea caa acc age atc ac ctt ect gag cca 493 Lys Wing Wing Gly Lys Val Ser Gln Thr Ser He Thr Leu Pro Glu Pro 130 135 140 10 gct atc gct gta aaa atc taaaacttca ccaggacaga taa 534 Ala He Ala Val Lys He 145 < 210 > 276 < 211 > 149 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 276 Val He He Thr Wing Gly He Leu Val Wing Thr Wing Thr Wing Leu Leu 15 1 5 10 15 Met He Thr Wing Val Ser Glu Ser Thr Tyr He Val He Ser Leu Wing 20 25 30 Gly Phe Ser Leu Tyr Gly Leu Gly Leu Gly Leu Phe Wing Thr Pro Val 35 40 45 Thr Asp Thr Wing Leu Gly Thr Leu Pro Lys Asp Arg Thr Gly Wing Gly 50 55 60 Wing Gly Val Phe Lys Met Ser Ser Ser Leu Gly Ala Wing Leu Gly He 20 65 70 75 80 Wing Be Ser Thr Ser Val Phe Leu Wing Leu Arg Asp Gly Thr Be He 85 90 95 Asn Be Asp Val Wing Leu Wing Gly Thr Val Being Leu Gly He Asn Val 100 105 110 Val Phe Wing Wing Thr Wing Thr He Thr Wing Wing Val Leu He Pro Lys 115 120 125 Wing Wing Gly Lys Val Ser Gln Thr Ser He Thr Leu Pro Glu Pro Wing 25 130 135 140 He Ala Val Lys He 145 < 210 > 277 < 211 > 586 < 212 > DNA < 213 > Corynebacterium glutam cum < 220 > < 221 > CDS < 222 > (101) .. (586) < 223 > RXN03151 < 400 > 277 ccgaacttgg agctttgctg ttggaggeag ccaattagtc ccaatgtaaa cgcactgggt 60 agtatttgtt taaccatcca cctcaaggag taaaacgcac gtg ctt tcc cac atc 115 Val Leu Ser His He 1 5 att gat gtc ctc gcc gac ccg atc gat ggc acc cca ctt gta ggc gcc 163 He Asp Val Leu Wing Asp Pro He Asp Gly Thr Pro Leu Val Gly Wing 10 15 20 gaa gat ttc tea cgg ttg gtg tct gaa tct ggg cat tcc tac gat gtt 211 Glu Asp Phe Ser Arg Leu Val Ser Glu Ser Gly His Ser Tyr Asp Val 25 30 35 gct cgt caa ggg tat gtc gtc acc gtc cg ggt ggc ggt ggt ctc cgc tat 259 Wing Arg Gln Gly Tyr Val Thr Leu Wing Gly Wing Gly Wing Glu Leu Arg Tyr 40 45 50 ggc tea gat gat gca cag atg gcg gat ggg cgg gaa acc ttc ctt tct 307 Ser Gly Asp Asp Wing Gln Met He Wing Asp Arg Glu Thr Phe Leu Ser 55 60 65 ggc ggt cac ttc gcg ccc ttc gtg gaa gct gtc acc gag cat gtt caa 355 Gly Gly His Phe Wing Pro Phe Val Glu Wing Val Thr Glu His Val Gln 70 75 80 85 gat gtc gtt gac cag gca ggc ctt age gat gac gca cag cca gtg gtc 403 Asp Val Val Asp Gln Wing Gly Leu Ser Asp Asp Wing Gln Pro Val Val 90 95 100 tgc gaa atc ggc gcg gga acc ggc tac tac tc tcc cat acc ctt gat 451 Cys Glu He Gly Wing Gly Thr Gly Tyr Tyr Leu Ser His Thr Leu Asp 105 110 115 tct gtt gca gga tct cgc gga att ggc att gac gtt tcc gtg cac gcc 499 Ser Val Ala Gly Ser Arg Gly He Gly He Asp Val Ser Val His Wing 120 125 130 gca aag cgt ttg gca aag tgt cac ect cgc gtc ggc gca gtc atc gcg 547 Wing Lys Arg Leu Wing Lys Cys His Pro Arg Val Gly Ala Val He Ala 135 140 145 _ | ^ ^^^^ gg¡¡g aac gca tgg gca cgc ctg ccg att gca gat aac tcc tcg 586 Asn Ala Trp Ala Arg Leu Pro He Ala Asp Asn Ser Ser 150 155 160 < 210 > 278 < 211 > 162 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 278 Val Leu Ser His He As Asp Val Leu Wing Asp Pro As Asp Gly Thr 1 5 10 15 Pro Leu Val Gly Wing Glu Asp Phe Ser Arg Leu Val Ser Glu Ser Gly 20 25 30 His Ser Tyr Asp Val Wing Arg Gln Gly Tyr Val Thr Leu Wing Gly Gly 35 40 45 Wing Gly Leu Arg Tyr Ser Gly Asp Asp Wing Gln Met He Wing Asp Arg • 50 55 60 Glu Thr Phe Leu Ser Gly Gly His Phe Wing Pro Phe Val Val Val Wing Val 65 70 75 80 Thr Glu His Val Gln Asp Val Val Asp Gln Wing Gly Leu Ser Asp Asp 85 90 95 Wing Gln Pro Val Val Cys Glu He Gly Wing Gly Thr Gly Tyr Tyr Leu 100 105 110 Ser His Thr Leu Asp Ser Val Wing Gly Ser Arg Gly He Gly He Asp 115 120 125 Val Ser Val His Wing Wing Lys Arg Leu Wing Lys Cys His Pro Arg Val 130 135 140 Gly Wing Val He Wing Wing Asn Wing Trp Wing Arg Leu Pro Wing Wing Asp Asn 145 150 155 160 Being < 210 > 279 < 211 > 543 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (1) .. (543) < 223 > RXN02832 < 400 > 279 cgc ggg cca gtg atg gat tat aca aat caa tea tta gta gt ttt ttc 48 Arg Gly Pro Val Met Asp Tyr Thr Asn Gln Ser Leu Val Ala Phe Phe 1 5 10 15 ttt aaa gca tta acg tea tat tta aag aaa cac aat tgt tta tat gtc 96 Phe Lys Wing Leu Thr Ser Tyr Leu Lys Lys His Asn Cys Leu Tyr Val 20 25 30 ctt gta gat cca tat tta att gaa aat tta cgc aat gca gac ggt gaa 144 Leu Val Asp Pro Tyr Leu He Glu Asn Leu Arg Asn Wing Asp Gly Glu 35 40 45 att gtt aaa tct tat gat aac cga gca ttt gtt aga aca atg gat aaa 192 He Val Lys Ser Tyr Asp Asn Arg Ala Phe Val Arg Thr Met Asp Lys 50 55 60 tta ggt tat aaa cac caa ggt ttc ect gta ggt tat gat tea atg age 240 Leu Gly Tyr Lys His Gln Gly Phe Pro Val Gly Tyr Asp Ser Met Ser 65 70 75 80 caa atc cgt tgg ctg tea gtg tta gatta aaa gat aag act gaa gac 288 Gln He Arg Trp Leu Ser Val Leu Asp Leu Lys Asp Lys Thr Glu Asp 85 90 95 caa ctt tta aaa gaa atg gat tat caa acg aga cgt aat att aaa aaa 336 Gln Leu Leu Lys Glu Met Asp Tyr Gln Thr Arg Arg Asn He Lys Lys 100 105 110 here tat gat att ggt gtc aaa act aaa acg tta acg att gat gaa acg 384 Thr Tyr Asp He Gly Val Lys Thr Lys Thr Leu Thr He Asp Glu Thr 115 120 125 caa act ttt ttc gac tta ttc cat atg gct gag gaa aag cac ggt ttc 432 Gln Thr Phe Phe Asp Leu Phe His Met Wing Glu Glu Lys His Gly Phe 130 135 140 aaa ttc cgt gag tta cca tac ttt gaa gaa atg caa aag tta tac gat 480 Lys Phe Arg Glu Leu Pro Tyr Phe Glu Glu Met Gln Lys Leu Tyr Asp 145 150 155 160 gac cac gcc atg tta aag ttg gcg tat att gat tta aac gag tat tta 528 Asp His Wing Met Leu Lys Leu Wing Tyr He Asp Leu Asn Glu Tyr Leu 165 170 175 aaa acg ttg caa tta 543 Lys Thr Leu Gln Leu 180 < 210 > 280 < 211 > 181 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 280 Arg Gly Pro Val Met Asp Tyr Thr Asn Gln Ser Leu Val Wing Phe Phe 1 5 10 15 Phe Lys Wing Leu Thr Ser Tyr Leu Lys Lys His Asn Cys Leu Tyr Val 20 25 30 Leu Val Asp Pro Tyr Leu He Glu Asn Leu Arg Asn Wing Asp Gly Glu 35 40 45 He Val Lys Ser Tyr Asp Asn Arg Wing Phe Val Arg Thr Met Asp Lys 50 55 60 Leu Gly Tyr Lys His Gln Gly Phe Pro Val Gly Tyr Asp Ser Met Ser 65 70 75 80 Gln He Arg Trp Leu Ser Val Leu Asp Leu Lys Asp Lys Thr Glu Asp 85 90 95 Gln Leu Leu Lys Glu Met Asp Tyr Gln Thr Arg Arn Asn He Lys Lys 100 105 110 Thr Tyr Asp He Gly Val Lys Thr Lys Thr Leu Thr He Asp Glu Thr 115 120 125 Gln Thr Phe Phe Asp Leu Phe His Met Wing Glu Glu Lys His Gly Phe 130 135 140 Lys Phe Arg Glu Leu Pro Tyr Phe Glu Glu Met Gln Lys Leu Tyr Asp 145 150 155 160 Asp His Wing Met Leu Lys Leu Wing Tyr He Asp Leu Asn Glu Tyr Leu 165 170 175 Lys Thr Leu Gln Leu 180 < 210 > 281 < 211 > 1539 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1516) < 223 > RXN00165 < 400 > 281 aaacctcccc gggcccggcg cgcgaccgtc caagatgccg gcgttggatg ccaaattatg 60 gactctcaaa gtggcgttgt cgcagcggcc gtggagcttt gtg gcg tct gct ggc 115 Val Wing Wing Wing Gly 1 5 atg gcg gcg tct ttt atc tgc aat ggg tta acg ect gtg att gtg ggt 163 Met Wing Wing Being Phe He Cys Asn Gly Leu Thr Pro Val He Val Gly October 15 20 aag gcg gtg gat gag gct att ggc acg age gat ctg cag cga ttg tgg 211 Lys Ala Val Asp Glu Ala He Gly Thr Ser Asp Leu Gln Arg Leu Trp 25 30 35 ttc tgg att gcc atg ttg gcg gtt ctt ttc tta acg gcg atg acg gtg 259 Phe Trp He Ala Met Leu Ala Val Leu Phe Leu Thr Ala Met Thr Val 40 45 50 aac tgg att gct cgg tac atg ttg gtg cgg age cag cag ttg gtc age 307 Asn Trp He Ala Arg Tyr Met Leu Val Arg Ser Gln Gln Leu Val Ser 55 60 65 cat gat ttg cgc atg ttg gtg act gat cgg att caa gat ccg cgt ggt 355 His Asp Leu Arg Met Leu Val Thr Asp Arg He Gln Asp Pro Arg Gly 70 75 80 85 gga aaa gct ttt cgc gag act ttg ttg gga ggt gcg tcg gcg att 403 Phe Ala Gly tea Lys Glu Ala Gly Gly Thr Arg Leu Leu Ala Ser Ser He 90 95 100 tcg gat acg cag cgg gtg ggc gat atc gtc atg atg acg gtg ttc ccg 451 10 Ser Asp Thr Gln Arg Val Gly Asp He Val Met Met Thr Val Phe Pro 105 110 115 gtg gcg gaa ttg gcg tcg att att tat ggc gcc gtg gtg atg tac age 499 Val Ala Glu Leu Ala Ser He He Tyr Gly Ala Val Val Met Tyr Ser 120 125 130 att aat ccg tgg ttg agt gtg gct gtg ctg att ggt gga ccg ctg ctg 547 He Asn Pro Trp Leu Ser Val Ala Val Leu He Gly Gly Pro Leu Leu 135 140 145 gtt gtg gtg gct att cag gtc tea aag ccg ttg cag aag cgt tcg ggt 595 15 Val Val Val Ala He Gln Val Ser Lys Pro Leu Gln Lys Arg Ser Gly 150 155 160 165 gct cgt cag cag gcg gtg gca cag gct gcg gct act gca act gat gtg 643 Wing Arg Gln Gln Wing Val Wing Gln Wing Wing Ala Thr Ala Thr Asp Val 170 175 180 gtg cag ggc ttg aga att ttg aag ggt tg ggc gcg att gtc acg gtg 691 Val Gln Gly Leu Arg He Leu Lys Gly Leu Gly Ala He Val Val Val 185 190 195 cgc cgt cgg tac gag gcg att tct ggt gag gct tat cgg aag acg gtt 739 20 Arg Arg Arg Tyr Glu Ala He Ser Gly Glu Wing Tyr Arg Lys Thr Val 200 205 210 cat gcg gat gct gcg gaa gct cgc ttg aat ggt gtc acc gat gcg gcg 787 His Wing Asp Ala Ala Glu Ala Arg Leu Asn Gly Val Thr Asp Ala Ala 215 220 225 ggc gcc atc ttt gtg tcc gcg ttg ggt att gga gca gga ttt ttg gcg 835 Gly Ala He Phe Val Ser Ala Leu Gly He Gly Ala Gly Phe Leu Ala 230 235 240 245 ctg caa ggt cag atg agt att ggt gat ttg atc acg gtt gtg gga ctc 883 25 Leu Gln Gly Gln Met Be He Gly Asp Leu He Thr Val Val Gly Leu ^ .aaBMai »250 255 260 aca cag ttt ttg atc atg ccg atg acc atg ctt ggt cga aat gtg gca 931 Thr Gln Phe Leu Met Met Pro Met Thr Met Leu Gly Arg Asn Val Ala 265 270 275 tcg cgc tgg gca tcg gcg gag gcg tcg gca aag cgt att agg gga gtg 979 Ser Arg Trp Wing Being Wing Glu Wing Being Wing Lys Arg He Arg Gly Val 280 285 290 ctc ggt gct gat ttt gag aga gtg tct gcg cat gat gcg gac aag gct 1027 Leu Gly Wing Asp Phe Glu Arg Val Ser Wing His Asp Wing Asp Lys Wing 295 300 305 gag gag att atc caa caa ctt gcc aaa ggt ttg acg gtt att cga ggc 1075 Glu Glu He He Gln Gln Leu Ala Lys Gly Leu Thr Val He Arg Gly 310 315 320 325 act gat gag cag ctc gtt gag gta tta gag cag ttg cca cgt act cgg 1123 Thr Asp Glu Gln Leu Val Glu Val Leu Glu Gln Leu Pro Arg Thr Arg 10 330 335 340 gtg att gtg gct ect cat gcg gcg gat ctt ttt gat caa agt gtc agg 1171 Val He Val Wing Pro His Wing Wing Asp Leu Phe Asp Gln Ser Val Arg 345 350 355 gac aat gtg cat ccc gtg gca gag gtc gcg gag aaa gcc att gaa gtt 1219 Asp Asn Val His Pr o Val Ala Glu Val Ala Glu Lys Ala He Glu Val 360 365 370 gcc tcc tgt gac gat att cca ggt ggt agt tcc aag att gtg ggc gag 1267 Wing Ser Cys Asp Asp He Pro Gly Gly Ser Ser Lys He Val Gly Glu 15 375 380 385 ggt gga cgg tg ggt ggt ggt ggt ggt cag cgc gtt gg gg ctg gct 1315 Gly Gly Arg Leu Leu Ser Gly Gly Glg Arg Glg Arg Val Wing Ala Leu Wing 390 395 400 405 cgg gcg att gct ttt gat cca gag gtg ttg gtg ctt caa gat ccc aca 1363 Arg Wing He Wing Phe Asp Pro Glu Val Leu Val Leu Gln Asp Pro Thr 410 415 420 acg gca gtg gat tct gtg acg gag caa aac att gct cag caa gtg gca 1411 Thr Ala Val Asp Ser Val Thr Glu Gln Asn He Wing Gln Gln Val Wing 20 425 430 435 gca cac cgt gca gga aaa gtg acc att gtg ttt agt gag gca ccc gcg 1459 Wing His Arg Wing Gly Lys Val Thr He Val Phe Ser Glu Wing Pro Wing 440 445 450 tgg agt gcg gtg gct gat caa cac gtt gag gca gct gct ttg cgg gag 1507 Trp Ser Ala Val Ala Asp Gln His Val Glu Ala Ala Ala Leu Arg Glu 455 460 465 gtt atg aaa tgagtgggga gacgtcgaaa age 1539 Val Met Lys 25 470 aaa > What is it? Q .i,. ... .. ..aa .... "" i ~ - • - &";>.,.,., ... .. ty .artimitli iimm ^ <210> 282 <211> 472 < 212> PRT <213> Corynebacterium glutamicum <400> 282 5 Val Wing Being Wing Gly Met Wing Wing Being Phe He Cys Asn Gly Leu Thr 1 5 10 15 Pro Val He Val Gly Lys Wing Val Asp Glu Wing He Gly Thr Ser Asp 20 25 30 Leu Gln Arg Leu Trp Phe Trp He Wing Met Leu Wing Val Leu Phe Leu 35 40 45 Thr Wing Met Thr Val Asn Trp He Wing Arg Tyr Met Leu Val Arg Ser 50 55 60 10 Gln Gln Leu Val Ser His Asp Leu Arg Met Leu Val Thr Asp Arg He 65 70 75 80 Gln Asp Pro Arg Gly Phe Wing Gly Lys Glu Arg Thr Wing Gly Gly Leu 85 90 95 Leu Ser He Wing Being Ser Asp Thr Gln Arg Val Gly Asp He Val Met 100 105 110 Met Thr Val Phe Pro Val Wing Glu Leu Wing Ser He He Tyr Gly Wing 115 120 125 15 Val Val Met Tyr Ser He Asn Pro Trp Leu Ser Val Wing Val Leu He 130 135 140 Gly Gly Pro Leu Leu Val Val Val Ala Wing Gln Val Ser Lys Pro Leu 145 150 155 160 Gln Lys Arg Ser Gly Wing Arg Gln Gln Wing Val Wing Gln Wing Wing Wing 165 170 175 Thr Wing Thr Asp Val Val Gln Gly Leu Arg He Leu Lys Gly Leu Gly 180 185 190 20 Wing He Val Thr Val Arg Arg Arg Tyr Glu Wing He Ser Gly Glu Wing 195 '200 205 Tyr Arg Lys Thr Val His Wing Asp Wing Wing Glu Wing Arg Leu Asn Gly 210 215 220 Val Thr Asp Wing Wing Gly Wing He Phe Val Be Ala Leu Gly He Gly 225 230 235 240 Wing Gly Phe Leu Wing Leu Gln Gly Gln Met Be He Gly Asp Leu He 245 250 255 25 Thr Val Val Gly Leu Thr Gln Phe Leu Met Met Pro Met Thr Met Leu mm m ^? á ^^^^ 260 265 270 Gly Arg Asn Val Wing Wing Arg Trp Wing Wing Wing Glu Wing Wing Wing 275 280 285 Arg He Arg Gly Val Leu Gly Wing Asp Phe Glu Arg Val Ser Wing His 290 295 300 - Asp Wing Asp Lys Wing Glu Glu He He Gln Gln Leu Wing Lys Gly Leu • 305 310 315 320 Thr Val He Arg Gly Thr Asp Glu Gln Leu Val Glu Val Leu Glu Gln 325 330 335 Leu Pro Arg Thr Arg Val He Val Wing Pro His Wing Wing Asp Leu Phe 340 345 350 Asp Gln Ser Val Arg Asp Asn Val His Pro Val Wing Glu Val Wing Glu 355 360 365 10 Lys Wing He Glu Val Wing Ser Cys Asp Asp He Pro Gly Gly Ser Ser 370 375 380 Lys He Val Gly Glu Gly Gly Arg Leu Leu Ser Gly Gly Gln Arg Gln • 385 390 395 400 Arg Val Ala Leu Ala Arg Ala He Ala Phe Asp Pro Glu Val Leu Val 405 410 415 Leu Gln Asp Pro Thr Thr Ala Val Asp Ser Val Thr Glu Gln Asn He 420 425 430 15 Wing Gln Gln Val Wing Wing His Arg Wing Gly Lys Val Thr He Val Phe 435 440 445 Ser Glu Wing Pro Wing Trp Ser Wing Val Wing Asp Gln His Val Glu Wing 450 455 460 Wing Ala Leu Arg Glu Val Met Lys 465 470 < 210 > 283 < 211 > 1470 20 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1447) < 223 > RXN01190 < 400 > 283 cagggttttg atgagaacaa cacacaccgc ttcaageatt ctgegaagaa tgatcaggcg 60 gcagcggggc aaggttgcgt ttggcgcatt etttttgggg atg tgg cag ctg tcg 115 25 Met Trp Gln Leu Ser -lilñtiti'ÉMliilr-ililÉi i T¡ -i i. rg - gaa gca ttg gtg ccg att gcg att ggt ttg atc gtt gat cat gcg gtt 163 Glu Ala Leu Val Pro He Wing He Gly Leu He Val Asp His Wing Val 10 15 20 ctc aca aaa gat ctc cgc cga tta gtg gtc ggg ctt gtc gtc gtt ttt gtt 211 Leu Thr Lys Asp Leu Arg Arg Leu Val Val Gly Leu Val Wing Phe Val 25 30 35 gtg ctg ttt gtg gtg ttg agt ttt tct aat cgt ttc ggt tcg cgc gcg 259 Val Leu Phe Val Val Leu Be Phe Ser Asn Arg Phe Gly Be Arg Wing 40 45 50 ttg aat agg gcc gtg aac ttt gaa tcc cat gcg ctc cgc gta gag gta 307 Leu Asn Arg Wing Val Asn Phe Glu Ser His Wing Leu Arg Val Glu Val 55 60 65 gcc gat cat gcg ttg aag aat ctg gat ccg cgc aat ttg gtg ect ggc 355 Wing Asp His Wing Leu Lys Asn Leu Asp Pro Arg Asn Leu Val Pro Gly 70 75 80 85 gag gtg atg tcg tcg cgg tcg ac gc gat gcg gat tct tcg acg cgt att 403 Glu Val Met Being Arg Being Thr Wing Asp Wing Asp Ser Being Thr Arg He 90 95 100 ttc ggg cag atc gga acc ggt gtt tcg gct gcg acg gga ttt ctt ggt 451 Phe Gly Gln He Gly Thr Gly Val Ser Ala Ala Thr Gly Phe Leu Gly 105 110 115 gca gcg acc tac ctg ttg atc agt gac tgg ctg gtc ggg ttg ttg gtg 499 Ala Ala Thr Tyr Leu Leu He Ser Asp Trp Leu Val Gly Leu Leu Val 120 125 130 ctt gtg ctg gta ccg atc att tcg gga gtg gtt gca ctg gct age aag 547 Leu Val Leu Val Pro He He Ser Gly Val Val Ala Leu Wing Ser Lys 135 140 145 ggc att tct aaa agg agt gtc acc cag gag aag ttg gcg gag tct 595 Gly He Ser Lys Arg Ser Val Thr Gln Gln Glu Lys Leu Wing Glu Ser 150 155 160 165 ggt gg gg gg gg agg gac atg atg ggg ctg cgc gtg atc aag gcg 643 Gly Wing Gln Wing Be Asp He Met Met Gly Leu Arg Val He Lys Wing 170 175 180 atc ggt ggc gag cgt tgg gcc gtg aag act ttt gaa aag gcg tcg cag 691 He Gly Gly Glu Arg Trp Wing Val Lys Thr Phe Glu Lys Wing Ser Gln 185 190 195 gca gcg tea gcg aga gcg gtt gtt gat act gca gtt gct tcg ggc aaa gtc 739 Wing Be Ala Arg Ala Ala Val Asp Thr Ala Ala Ala Be Gly Lys Val 200 205 210 gct ggt att ggt gag ttg tcc att gcg gtg aat ttg gct gcg gtg ttg 787 Wing Gly He Gly Glu Leu Ser He Wing Val Asn Leu Wing Ala Val Leu 215 220 225 ...,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, gtg ggt gtg gcg gtg tat ttg tea gag ccg att cgc 883 Leu He Wing He Val Gly Val Wing Val Tyr Leu Ser Glu Pro He Arg 250 255 260 5 • ttg ctg age aac tcg att aat gcc tea gct att gcg cac ggt gca gcg 931 Leu Leu Ser Asn Ser He Asn Wing Wing Wing Wing His Gly Wing Wing 265 270 275 gag cgg gtg gct aat ttc tta aac ctc gac gaa tct cag gca cag tac 979 Glu Arg Val Wing Asn Phe Leu Asn Leu Asp Glu Ser Gln Ala Gln Tyr 280 285 290 gaa age age gaa aca atc aat gac ggc gaa ttc ctc gtc atc gtg ccc 1027 Glu Ser Glu Thr He Asn Asp Gly Glu Phe Leu Val He Val Pro 295 300 305 10 cca gcc age acg ctt cca cac ggc gac aat atc ttg gct here ect cat 1075 Pro Wing Thr Leu Pro His Gly Asp Asn He Leu Wing Thr Pro His 310 315 320 325 gct gcc gac att ttc gaa ggt acc ttg cgg tea aat att tcc atg aat 1123 Wing Wing Asp He Phe Glu Gly Thr Leu Arg Ser Asn He Ser Met Asn 330 335 340 cat gag gac aac gtg cca att gat ccg cag gta att cgc gct tct ggt 1171 His Glu Asp Asn Val Pro He Asp Pro Gln Val He Arg Wing Ser Gly 3 45 350 355 15 ctg act gac atc att gag gtg gac gga ctt gat gcg ccg gtg cgc gat 1219 Leu Thr Asp He He Glu Val Asp Gly Leu Asp Ala Pro Val Arg Asp 360 365 370 acg gga age aat tta tcg ggt ggg cag cgt cag cga gtg gct tcg gcc 1267 Thr Gly Ser Asn Leu Ser Gly Gly Gln Arg Gln Arg Val Wing Ala Leu Wing 375 380 385 • agg gcg ttg cat gca gac gcg gaa gta ctg gtg ctg atg gat cca acc 1315 Arg Ala Leu His Ala Asp Wing Glu Val Leu Val Leu Met Asp Pro Thr 390 395 400 405 20 age gcg gtg gat gtg gtg acg gag gtg tct atc gcg cag ggg att aag 1363 Ser Ala Val Asp Ser Val Thr Glu Val Ser He Ala Gln Gly He Lys 410 415 420 cag ctg cga gca ggc aaa acc acc att gtg gtg agt tct tcg ccc gcg 1411 Gln Leu Arg Wing Gly Lys Thr Thr He Val Val Ser Ser Ser Wing 425 430 435 ttt tac aac ttg gcg gat cgg gtg att tea cat gtc taatttgatg 1457 Phe Tyr Asn Leu Wing Asp Arg Val He Ser His Val 440 445 25 -'iiiíii -tfíifrttf '-Hiíi-il 1 f' gcatcatcga cac 1470 < 210 > 284 < 211 > 449 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 284 Met Trp Gln Leu Ser Glu Ala Leu Val Pro He Wing He Gly Leu He 1 5 10 15 Val Asp His Wing Val Leu Thr Lys Asp Leu Arg Arg Leu Val Val Gly 20 25 30 Leu Val Wing Phe Val Val Leu Phe Val Val Leu Ser Phe Ser Asn Arg 35 40 45 Phe Gly Ser Arg Ala Leu Asn Arg Ala Val Asn Phe Glu Ser His Wing 50 55 60 Leu Arg Val Glu Val Wing Asp His Wing Leu Lys Asn Leu Asp Pro Arg 65 70 75 80 Asn Leu Val Pro Gly Glu Val Met Ser Arg Be Thr Wing Asp Wing Asp 85 90 95 Be Ser Thr Arg He Phe Gly Gln He Gly Thr Gly Val Be Ala Wing 100 105 110 Thr Gly Phe Leu Gly Wing Wing Thr Tyr Leu Leu He Ser Asp Trp Leu 115 120 125 Val Gly Leu Leu Val Leu Val Leu Val Pro He He Ser Gly Val Val 130 135 140 Ala Leu Ala Ser Lys Gly He Ser Lys Arg Ser Val Thr Gln Gln Glu 145 150 155 160 Lys Leu Ala Glu Ser Gly Ala Gln Ala Be Asp Met Met Gly Leu 165 170 175 Arg Val He Lys Ala He Gly Gly Glu Arg Trp Ala Val Lys Thr Phe 180 185 190 Glu Lys Ala Ser Gln Ala Ala Ala Ala Ala Ala Asp Thr Ala Ala 195 205 Wing Be Gly Lys Val Wing Gly He Gly Glu Leu Ser He Wing Val Asn 210 215 220 Leu Wing Wing Val Leu Leu Wing Gly Trp Arg Val Thr Thr Gly Glu 225 230 235 240 Leu Gly Pro Gly Gln Leu He Wing He Val Val Val Wing Val Tyr Leu 245 250 255 Ser Glu Pro He Arg Leu Leu Ser Asn Ser He Asn Wing Ser Wing He 260 265 270 Wing His Gly Wing Wing Wing Arg Val Wing Asn Phe Leu Asn Leu Asp Glu 275 280 285 Being Gln Wing Gln Tyr Glu Being Ser Glu Thr He Asn Asp Gly Glu Phe 290 295 300 Leu Val He Pro Val Pro Pro Wing Ser Thr Leu Pro His Gly Asp Asn He 305 310 315 320 Leu Wing Thr Pro His Wing Wing Asp He Phe Glu Gly Thr Leu Arg Ser 325 330 335 Asn He Met Met Asn His Glu Asp Asn Val Pro He Asp Pro Gln Val 340 345 350 He Arg Wing Ser Gly Leu Thr Asp He He Glu Val Asp Gly Leu Asp 355 360 365 Wing Pro Val Arg Asp Thr Gly Ser Asn Leu Ser Gly Gly Gln Arg Gln 370 375 380 Arg Val Ala Leu Ala Arg Ala Leu His Ala Asp Ala Glu Val Leu Val 385 390 395 400 Leu Met Asp Pro Thr Be Wing Val Asp Ser Val Thr Glu Val Ser He 405 410 415 Wing Gln Gly He Lys Gln Leu Arg Wing Gly Lys Thr Thr He Val Val 420 425 430 Ser Ser Ser Pro Wing Phe Tyr Asn Leu Wing Asp Arg Val He Ser His 435 440 445 Val < 210 > 285 < 211 > 1368 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1345) < 223 > RXN01102 < 400 > 285 attctatggt tgttgggaga gatgacttaa tttggaatca cgggctttaa cacgcgctga 60 cattgagcaa cttcccagca tgtggaaaag cccaggtttc gtg gtc gtc ctc gtg 115 Val Ala Val Leu Val 1 5 gcg gtt gca gcg tcc ggc agt tgg tea ctc ctt ctt ccc gtc gta 163 Ala Ala Ala Ala Ala Phe Gly Ser Trp Ser Leu Leu Leu Pro Val Val 10 15 20 ccg cta gcg gtc ctc aac aac ggc gga tea age gct gcc gcc gc gcc 211 Pro Leu Ala Val Leu Asn Asn Gly Gly Ser Ser Ala Ala Wing Ala Gly 25 30 35 acc act ggc atc tcc atg gc gct gtg gtg atc act cag att ttc act 259 Thr Thr Gly He Phe Met Wing Wing Thr Val He Thr Gln He Phe Thr 40 45 50 ccc gcg ctg cgg aaa att ggc tac acc cca gtg atg gct tcc gcc 307 Pro Ala Ala Leu Arg Lys He Gly Tyr Thr Pro Val Met Wing Ala Phe Wing 55 60 65 gca ttc atg ctg ggt gtg cca gcc atc ggg tac atc ttc age gtc gag 355 Wing Phe Met Leu Gly Val Pro Wing He Gly Tyr He Phe Ser Val Glu 70 75 80 85 cca att cca gtg ctg gta gtg tcc gca ctt cga gga att ggg ttc ggt 403 Pro He Pro Val Leu Val Val Ser Ala Leu Arg Gly He Gly Phe Gly 90 95 100 gcg ctc acc gtc gca gaa tct gcg ttg gtg gct gaa ctc gtt ccc gta 451 Ala Leu Thr Val Ala Glu Ser Ala Leu Val Ala Glu Leu Val Pro Val 105 110 115 cgc ttc tg ggc aaa gct tct gga tg ggc ggc gta ttt att ggc ctt 499 Arg Phe Leu Gly Lys Wing Gly Met Leu Gly Val Phe He Gly Leu 120 125 130 tcc caa atg ctt ttc ctg ect gcc ggg ttg gcg tta ggt gac caa ttt 547 Ser Gln Met Leu Phe Leu Pro Ala Gly Leu Ala Leu Gly Asp Gln Phe 135 140 145 ggc tac aac gtg gtc tat gtt tta ggt gcc gtt atc gca cta gtt gca 595 Gly Tyr Asn Val Val Tyr Val Leu Gly Ala Val He Ala Ala Leu Val Ala 150 155 160 165 gcg gtg atg tgt ctg cgt att ccg cag gtt aag gca gcg gca aag cag 643 Ala Val Met Cys Leu Arg He Pro Gln Val Lys Ala Ala Ala Lys Gln 170 175 180 caca cca cag gtg age gaa cag gag cgt tct gtt tcc acc tgg aag ttg 691 Gln Pro Gln Val Ser Glu Gln Glu Arg Ser Val Ser Thr Trp Lys Leu 185 190 195 gtg ctg gtt ccc tcc ttg gct gtt acc agt ttg tea atg act ttt ggc 739 Val Leu Val Pro Ser Leu Wing Val Thr Ser Leu Ser Met Thr Phe Gly 200 205 210 gca gtg tct tea ttc ctt cca gct gca gtc att gag tta gat cca gga 787 Wing Val Ser Ser Phe Leu Pro Ala Wing Val He Glu Leu Asp Pro Gly 215 220 225 tta ggt gct gca tta gcg ggt att att tta tcc att acc ggt ggt tct 835 iinii ir riÉÉ Leu Gly Ala Ala Leu Ala Gly He He Leu Ser He Thr Gly Gly Ser 230 235 240 245 tea atg gtg ttc cgc tac ctg tcc ggc gtt atc gct gac cgc cgc ggt 883 Ser Met Val Phe Arg Tyr Leu Ser Gly Val He Wing Asp Arg Arg Gly 250 255 260 gtg ect ggt acc acg atg att ect gct cag atc att ggg ttc tta ggt 931 Val Pro Gly Thr Thr Met He Pro Wing Gln He He Gly Phe Leu Gly 265 270 275 gtc gtt tta atc acc gtc aca atc ttc caa ggc tgg tcc gtg tgg ctt 979 Val Val Leu He Thr Val Thr He Phe Gln Gly Trp Ser Val Trp Leu 280 285 290 ttg att ata ggt gca gtg atg ttt ggt ggt gct ttt ggc atg gtg caa 1027 Leu He He Gly Wing Val Met Phe Gly Gly Wing Phe Gly Met Val Gln 295 300 305 aac gaa gcg ttg ctt tea atg ttt ttc cgg ctt cctct act aga gtc 1075 Asn Glu Ala Leu Leu Ser Met Phe Phe Arg Leu Pro Arg Thr Arg Val 310 315 320 325 tcc gaa gcc tcc gcc atc tgg aat atc gcc ttt gat tcg gga aca gga 1123 Ser Glu Wing Being Wing He Trp Asn He Wing Phe Asp Ser Gly Thr Gly 330 335 340 atc gga age ttc ctc ctt ggc ata gtt gcc gca tcg ctt gct tac agt 1171 He Gly Being Phe Leu Leu Gly He Val Wing Wing Being Leu Wing Tyr Being 345 350 355 ggt gct ttt ggt tcc gga gcc gtg gtg att ttg ttt gga atc gtt ttg 1219 Gly Wing Phe Gly Ser Gly Wing Val Val He Leu Phe Gly He Val Leu 360 365 370 acc acc gcc gat cga atc att ggg cgg cac cgc att act gaa tac aac 1267 Thr Thr Wing Asp Arg He He Gly Arg His Arg He Thr Glu Tyr Asn 375 380 385 aac acc cgc gcg cgt ttg cgc cag gtg cca gtc gct cgg cgt gca gtg 1315 Asn Thr Arg Ala Arg Leu Arg Gln Val Pro Val Wing Arg Arg Wing Val 390 395 400 405 caa ggg ctg cgc aac agg cgc aaa gat cgc taaaacgctt ttcgacgcca 1365 Gln Gly Leu Arg Asn Arg Arg Lys Asp Arg 410 415 ccc 1368 < 210 > 286 < 211 > 415 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 286 Val Ala Val Val Leu Val Ala Ala Ala Ala Ala Phe Gly Ser Trp Ser Leu 10 15 Leu Leu Pro Val Val Pro Leu Ala Val Leu Asn Asn Gly Gly Be Ser 20 25 30 Wing Val Wing Gly Wing Thr Thr Gly He Phe Met Wing Wing Thr Val He 35 40 45 Thr Gln He Phe Thr Pro Wing Ala Leu Arg Lys He Gly Tyr Thr Pro 50 55 60 Val Met Wing Phe Wing Wing Phe Met Leu Gly Val Pro Wing He Gly Tyr 65 70 75 80 He Phe Ser Val Glu Pro He Pro Val Leu Val Val Ser Ala Leu Arg 85 90 95 Gly He Gly Phe Gly Ala Leu Thr Val Ala Glu Ser Ala Leu Val Ala 100 105 110 Glu Leu Val Pro Val Arg Phe Leu Gly Lys Ala Ser Gly Met Leu Gly 115 120 125 Val Phe He Gly Leu Ser Gln Met Leu Phe Leu Pro Wing Gly Leu Wing 130 135 140 Leu Gly Asp Gln Phe Gly Tyr Asn Val Val Tyr Val Leu Gly Wing Val 145 150 155 160 He Ala Ala Leu Ala Ala Ala Met Cys Leu Arg He Pro Gln Val Lys 165 170 175 Ala Ala Ala Glys Gln Pro Gln Val Ser Gllu Glu Arg Ser Val 180 185 190 Ser Thr Trp Lys Leu Val Leu Val Pro Ser Leu Ala Val Thr Ser Leu 195 200 205 Ser Met Thr Phe Gly Ala Val Ser Ser Phe Leu Pro Ala Ala Val He 210 215 220 Glu Leu Asp Pro Gly Leu Gly Ala Ala Leu Ala Gly He He Leu Ser 225 230 235 240 He Thr Gly Gly Ser Ser Met Val Phe Arg Tyr Leu Ser Gly Val He 245 250 255 Wing Asp Arg Arg Gly Val Pro Gly Thr Thr Met He Pro Wing Gln He 260 265 270 He Gly Phe Leu Gly Val Val Leu He Thr Val Thr He Phe Gln Gly 275 280 285 Trp Ser Val Trp Leu Leu He He Gly Ala Val Met Phe Gly Gly Ala 290 295 300 Phe Gly Met Val Gln Asn Glu Ala Leu Leu Ser Met Phe Phe Arg Leu 305 310 315 320 Pro Arg Thr Arg Val Ser Glu Wing Be Wing He Trp Asn He Wing Phe 325 330 335 Asp Ser Gly Thr Gly He Gly Ser Phe Leu Leu Gly He Val Wing Wing 340 345 350 Ser Leu Wing Tyr Ser Gly Wing Phe Gly Ser Gly Wing Val Val He Leu 355 360 365 Phe Gly He Val Leu Thr Thr Wing Asp Arg He He Gly Arg His Arg 370 375 380 He Thr Glu Tyr Asn Asn Thr Arg Ala Arg Leu Arg Gln Val Pro Val 385 390 395 400 Ala Arg Arg Ala Val Gln Gly Leu Arg Asn Arg Arg Lys Asp Arg 405 410 415 < 210 > 287 < 211 > 348 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (325) < 223 > RXN00788 < 400 > 287 cgcatccctc tagttttcca tcacctcaat gaacggcgct aactccggtt cattgcgcaa 60 ttgatccagc actgcttgca gtgaggcctc attagttggc atg gcc tcc tcc atc 115 Met Wing Being Ser I 5 aac atc gga gtg ttc aac ctt gga aat gct gtt gcc ggg tgg ctt gct 163 Asn He Gly Val Phe Asn Leu Gly Asn Ala Ala Ala Trp Leu Ala 10 15 20 ggt gca acc atc acc act tcc ctt gga ctc aca tea gcc gga tta gtt 211 Gly Ala Thr He Thr Thr Ser Leu Gly Leu Thr Ser Ala Gly Leu Val 25 30 35 ggc ggt ttg atg acg tcc ctc gga cta gtg ttg gcc atc gtg gct gtg 259 Gly Gly Leu Met Thr Ser Leu Gly Leu Val Leu Wing He Val Wing Val 40 45 50 gtt ttg cgt cga aaa gcg caa ggc acc gcg gcg acc atc age gtt gtg 307 Val Leu Arg Arg Lys Wing Gln Gly Thr Gln Wing Thr He Ser Val Val 55 60 65 gag cac cag ccc gcc caa taaataattt ctctcttcta att 348 Glu His Gln Pro Ala Gln 70 75 .i. ^ ..,. "., .._ .. < 210 > 288 < 211 > 75 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 288 Met Wing Being Ser He Asn He Gly Val Phe Asn Leu Gly Asn Wing Val 1 5 10 15 Wing Wing Trp Leu Wing Gly Wing Thr He Thr Thr Ser Leu Gly Leu Thr 20 25 30 Wing Wing Gly Leu Val Gly Gly Leu Met Thr Being Leu Gly Leu Val Leu 35 40 45 Wing He Val Wing Val Val Leu Arg Arg Lys Wing Gln Gly Thr Gln Wing 50 55 60 Thr He Ser Val Val Glu His Gln Pro Wing Gln 65 70 75 < 210 > 289 < 211 > 1764 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1741) < 223 > RXN02H9 < 400 > 289 ttcggtccgc tctggcaaaa atggctggct gccacctcgg cgcagcagct taagggctgg 60 gcttaaattg cttgtcgacg cctagtgcca caatggagac atg acc gaa aca ctt 115 Met Thr Glu Thr Leu 1 5 gtg gtg aat ggc ctt gca ggc ggc tat ggg cac cgc aca tta ttt aac 163 Val Val Asn Gly Leu Ala Gly Gly Tyr Gly His Arg Thr Leu Phe Asn 10 15 20 gat gtg aat ctc acc gta gct gcc gcg gat gtc gtg ggc gtt gtc ggc 211 Asp Val Asn Leu Thr Val Wing Wing Gly Asp Val Val Gly Val Val Gly 25 30 35 gtc aat ggc gct ggt aaa tcc ctg gt gt gt gt gt gt gt gt gtc gtc 259 Val Asn Gly Ala Gly Lys Ser Thr Phe Leu Lys He Leu Wing Gly Val 40 45 50 gaa aag cca gt gct gga act atc gcg ctt tcg cca gcc gat gct ttt 307 Glu Lys Pro Leu Wing Gly Thr He Wing Leu Ser Pro Wing Asp Wing Phe 55 60 65 gtg ggc tac tgc cca cag gaa cac ac cgc acg tct gga gag acg atc 355 Val Gly Tyr Leu Pro Gln Glu His Thr Arg Thr Ser Gly Glu Thr He 70 75 80 85 gca gtt tac att gct cgt cga acc ggc tgc caa gct gca aca act gcc 403 Wing Val Tyr He Wing Arg Arg Thr Gly Cys Gln Wing Ala Thr Thr Wing 90 95 100 atg gat gac acc gcc gac gcg ttt ggt gcg gat cca gac aac gct gcc 451 • Met Asp Asp Thr Wing Glu Wing Phe Gly Wing Asp Pro Asp Asn Ala Wing 105 110 115 ttg gcc gat gca tac gcc gag gcg ctg gat cgg tgg atg gcc agt ggc 499 Leu Wing Asp Wing Tyr Wing Glu Wing Leu Asp Arg Trp Met Wing Ser Gly 120 125 130 gca gcc gat ttg gat gaa cgc atc ccc atc gtg ctc gct gat gt ggc 547 Wing Wing Asp Leu Asp Glu Arg He Pro He Val Leu Wing Asp Leu Gly 135 140 145 10 ttt gag ctt ccc acc tcg acg ctg atg gaa gga ctt tea ggc ggg cag 595 Phe Glu Leu Pro Thr Ser Thr Leu Met Glu Gly Leu Ser Gly Gly Gln 150 155 160 165 gca gcc cgg gtc ggg ctg gcg gcg tta ctg ttg tea cgt ttt gac att 643 Ala Ala Arg Val Gly Leu Ala Ala Leu Leu Leu Ser Arg Phe Asp He 170 175 180 gtg ctt ctc gac gag ccc acc aac gat ttg gat ctc gac ggt ctt gag 691 Val Leu Leu Asp Glu Pro Thr Asn Asp Leu Asp Leu Asp Gly Leu Glu 185 190 195 15 caa ctg gag aat ttt gtt cag ggg ctt cgc ggg gga gtc gta ctg gtc 739 Gln Leu Glu Asn Phe Val Gln Gly Leu Arg Gly Gly Val Val Leu Val 200 205 210 age cat gat cgt gag ttt ctt tcc agg tgt gtg acc act gtg ctg gaa 787 Ser His Asp Arg Glu Phe Leu Ser Arg Cys Val Thr Thr Val Leu Glu 215 220 225 ctc gat ctg cac caa aat tcc cac cat gtt tat ggc ggt gga tat gat 835 • Leu Asp Leu His Gln Asn Ser His His Val Tyr Gly Gly Gly Tyr Asp 230 235 240 245 20 tcc tac ctt gag gaa cgc gca gtg cta cgc cag cac gcc cgt gac caa 883 Ser Tyr Leu Glu Glu Arg Ala Val Leu Arg Gln His Wing Arg Asp Gln 250 255 260 tat gag gaa ttt gcg gaa aag aag agate gac ctt gtg gca cgt gct cga 931 Tyr Glu Glu Phe Wing Glu Lys Lys Lys Asp Leu Val Wing Arg Wing Arg 265 270 275 acg cag cgt gaa tgg tct agt cac ggt gtc cgc aat gct att aaa cgt 979 Thr Gln Arg Glu Trp Ser Ser His Gly Val Arg Asn Ala He Lys Arg 280 285 290 25 gca ect gac aac gac aaa ctt cgg aag aaa gcc gct gcg gaa tcc agt 1027 - > «» - - Wing Pro Asp Asn Asp Lys Leu Arg Lys Lys Wing Wing Wing Glu Ser Ser 295 300 305 gaa aag cag gct caa aaa gtc cgc cag atg gaa age cgc atc gct cgg 1075 Glu Lys Gln Wing Gln Lys Val Arg Gln Met Glu Ser Arg He Wing Arg 310 315 320 325 tta gaa gaa gtt gaa gag cca cgt aaa gaa tgg aaa ctg cag ttc age 1123 Leu Glu Glu Val Glu Glu Pro Arg Lys Glu Trp Lys Leu Gln Phe Ser 330 335 340 gtc ggt aag gcg tcg cgg tea agt tct gtt gtt tc tg aat gat 1171 Val Gly Lys Wing Being Arg Being Ser Val Val Ser Thr Leu Asn Asp 345 350 355 gca age ttc acc ggc cat gtc gat ttc acc ttg gga cca gta tcc atc caa 1219 Wing Being Phe Thr Gln Gly Asp Phe Thr Leu Gly Pro Val He Gln 360 365 370 gta aat gct ggc gat cgc att ggc atc aca gga ccc aac ggt gct ggt 1267 Val Asn Wing Gly Asp Arg He Gly He Thr Gly Pro Asn Gly Ala Gly 375 380 385 aaa tcc ctg cgc gga cta gga tta gga aac caa gaa ccc acc age 1315 Lys Ser Thr Leu Leu Arg Gly Leu Leu Gly Asn Gln Glu Pro Thr Ser 390 395 400 405 ggt act gcc acg atg ggc acg age gtg gcg atc gga gaa atc gat cag 1363 Gly Thr Wing Thr Met Gly Thr Ser Val Wing He Gly Glu He Asp Gln 410 415 420 gca cga gcg tta ctt gat cca cag ttg cca ctg att tct gcg ttt gaa 1411 Ala Arg Ala Leu Leu Asp Pro Gln Leu Pro Leu He Ser Wing Phe Glu 425 430 435 aag cat gtt cca gac tta ccg atc agt gag gtg cgc aca ctc ctc gcg 1459 Lys His Val Pro Asp Leu Pro He Ser Glu Val Arg Thr Leu Leu Wing 440 445 450 aaa ttt ggg ctg aat gat aat cat gtg gaa cgg gac gtc gaa aag cta 1507 Lys Phe Gly Leu Asn Asp Asn His Val Glu Arg Asp Val Glu Lys Leu 455 460 465 tct ect ggc gag cgc acg cgc gcc gga ctt gcg ctg cta cag gtg cgg 1555 Ser Pro Gly Glu Arg Thr Arg Wing Gly Leu Wing Leu Leu Gln Val Arg 470 475 480 485 ggc gtc aac gtg ctt gtt ctt gat gag ccc acc aac cac ctt gac ctg 1603 Gly Val Asn Val Leu Val Leu Asp Glu Pro Thr Asn His Leu Asp Leu 490 495 500 gag gcc atc gag caa ttg gag cag gcg ttg gcc tcg tat gat ggt gtg 1651 Glu Wing He Gllu Gln Leu Glu Gln Ala Leu Wing Ser Tyr Asp Gly Val 505 510 515 ttg ctg ctg gt c acg cac gat cgt cgc atg ttg gac gct ggc cag acc 1699 Leu Leu Leu Val Thr His Asp Arg Arg Met Leu Asp Ala Val Gln Thr 520 525 530 aat cgt cgt tgg cat gtc gag gct ggc gaa gtt agg gag cta 1741 Asn Arg Arg Trp His Val Glu Ala Gly Glu Val Arg Glu Leu 535 540 545 taaccgtttc cgtattgatg cca 1764 • < 210 > 290 < 211 > 547 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 290 Met Thr Glu Thr Leu Val Val Asn Gly Leu Ala Gly Gly Tyr Gly His 1 5 10 15 Arg Thr Leu Phe Asn Asp Val Asn Leu Thr Val Wing Wing Gly Asp Val 20 25 30 10 Val Gly Val Val Gly Val Asn Gly Ala Gly Lys Ser Thr Phe Leu Lys 35 40 45 He Leu Wing Gly Val Glu Lys Pro Leu Wing Gly Thr He Wing Leu Ser 50 55 60 Pro Wing Asp Wing Phe Val Gly Tyr Leu Pro Gln Glu His Thr Arg Thr 65 70 75 80 Ser Gly Glu Thr He Wing Val Tyr He Wing Arg Arg Thr Gly Cys Gln 85 90 95 15 Wing Wing Thr Thr Wing Wing Asp Asp Thr Wing Wing Wing Phe Gly Wing Asp 100 105 110 Pro Asp Asn Wing Wing Leu Wing Asp Wing Tyr Wing Glu Wing Leu Asp Arg 115 120 125 Trp Met Wing Ser Gly Wing Wing Asp Leu Asp Glu Aig He Pro He Val 130 135 140 Leu Wing Asp Leu Gly Phe Glu Leu Pro Thr Ser Thr Leu Met Glu Gly 145 150 155 160 20 Leu Ser Gly Gly Gln Ala Ala Arg Val Gly Leu Ala Ala Leu Leu Leu 165 170 175 Be Arg Phe Asp He Val Leu Leu Asp Glu Pro Thr Asn Asp Leu Asp 180 185 190 Leu Asp Gly Leu Glu Gln Leu Glu Asn Phe Val Gln Gly Leu Arg Gly 195 200 205 Gly Val Val Leu Val Ser His Asp Arg Glu Phe Leu Ser Arg Cys Val 210 215 220 25 * JtÉj * tt- ^ • - '• •' "- - - *" *** - Thr Thr Val Leu Glu Leu Asp Leu His Gln Asn Ser His His Val Tyr 225 230 235 240 Gly Gly Gyr Tyr Asp Ser Tyr Leu Glu Glu Arg Wing Val Leu Arg Gln 245 250 255 His Wing Arg Asp Gln Tyr Glu Glu Phe Wing Glu Lys Lys Lys Asp Leu 260 265 270 Val Wing Arg Wing Arg Thr Gln Arg Glu Trp Ser Ser His Gly Val Arg 275 280 285 Asn Ala He Lys Arg Ala Pro Asp Asn Asp Lys Leu Arg Lys Lys Wing 290 295 300 Wing Wing Glu Ser Ser Glu Lys Gln Wing Gln Lys Val Arg Gln Met Glu 305 310 315 320 Ser Arg He Wing Arg Leu Glu Glu Val Glu Glu Pro Arg Lys Glu Trp 325 330 335 10 Lys Leu Gln Phe Ser Val Gly Lys Wing Ser Arg Ser Ser Val Val 345 345 350 Ser Thr Leu Asn Asp Wing Ser Phe Thr Gln Gly Asp Phe Thr Leu Gly 355 360 365 Pro Val Ser He Gln Val Asn Wing Gly Asp Arg He Gly He Thr Gly 370 375 380 Pro Asn Gly Wing Gly Lys Ser Thr Leu Leu Arg Gly Leu Leu Gly Asn 385 390 395 400 15 Gln Glu Pro Thr Ser Gly Thr Wing Thr Met Gly Thr Ser Val Wing He 405 410 415 Gly Glu He Asp Gln Ala Arg Ala Leu Leu Asp Pro Gln Leu Pro Leu 420 425 430 He Ser Wing Phe Glu Lys His Val Pro Asp Leu Pro He Ser Glu Val 435 440 445 Arg Thr Leu Leu Wing Lys Phe Gly Leu Asn Asp Asn His Val Glu Arg 450 455 460 20 Asp Val Glu Lys Leu Ser Pro Gly Glu Arg Thr Arg Wing Gly Leu Wing 465 470 475 480 Leu Leu Gln Val Arg Gly Val Asn Val Leu Val Leu Asp Glu Pro Thr 485 490 495 Asn His Leu Asp Leu Glu Wing He Glu Gln Leu Glu Gln Wing Ala Leu Wing 500 505 510 Ser Tyr Asp Gly Val Leu Leu Val Thr His Asp Arg Arg Met Leu 515 520 525 25 Asp Ala Val Gln Thr Asn Arg Arg Trp His Val Glu Ala Gly Glu Val 530 535 540 Arg Glu Leu 545 < 210 > 291 5 < 211 > 1638 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (1615) < 223 > RXN01605 < 400 > 291 attgaagtca gcgccgagga agccgcgagg gaatcagacg acgggcacct actacttcgg 60 10 cattgaaatt ccgaagaact tcagcgattc tattgccagc gtg acc age gat tea 115 Val Thr ser Asp Ser 1 5 ccc gcg cca gca acc gtc aac gcg gta tcc aac aac age aac ggc ttc 163 • Pro Wing Pro Wing Thr Val Asn Wing Val Phe Asn Asn Ser Asn Gly Phe 10 15 20 att gcc tcc atg ctg ggc aac cag gtg gtc aac act gtt gtg gag acc 211 He Wing Being Met Leu Gly Asn Gln Val Val Asn Thr Val Val Glu Thr 25 30 35 5 atg gac acg gaa ttc ggc gtc cgc att gtg gat aac atg ctc gtc ggt 259 Met Asp Thr Glu Phe Gly Val Arg He Val Asp Asn Met Leu Val Gly 40 45 50 ttc tcc acc ttg ggc gac ggc atg aac caa gcc gcc gaa ggt gcc act 307 Phe Ser Thr Leu Gly Asp Gly Met Asn Gln Wing Wing Glu Gly Wing Thr 55 60 65 acg ctc age gat ggc gtc ggt tcc gcc aac gac ggt gca gtt cag ctt 355 Thr Leu Ser Asp Gly Val Gly Ser Wing Asn Asp Gly Wing Val Gln Leu 70 75 80 85 20 gcc gac ggc gcg gtc acc ctc cgc gac ggc atc gca agt gcc aat gag 403 Wing Asp Gly Wing Val Thr Leu Arg Asp Gly He Wing Being Wing Asn Glu 90 95 100 ggt gcg caa tcg ctt gcc gac ggc gcc age cag ctc gac acc ggc ctc 451 Gly Wing Gln Ser Leu Wing Asp Gly Wing Wing Gln Leu Asp Thr Gly Leu 105 110 115 ggc tcc gcg gct aca ggc age caa acg ctc gcc gac ggt cta tcc age 499 Gly Ser Wing Wing Thr Gly Ser Gln Thr Leu Wing Asp Gly Leu Ser Ser 120 125 130 25 ctg tct gcg ggc acc gcc caa cta ggc caa ggc gca acc cag gtt tea 547 Leu Ser Wing Gly Thr Wing Gln Leu Gly Gln Gly Wing Thr Gln Val Ser 135 140 145 gat ggc gtg ggc caa ctt gtc gac ca gta gca cca ctg acc gcc tat 595 Asp Gly Val Gly Gln Leu Val Asp Gln Val Ala Pro Leu Thr Wing Tyr 150 155 160 165 gtt cca gac atc aac tct cag ttg atc acc ctg cgc gac ggc gca gcc 643 Val Pro Asp He Asn Ser Gln Leu He Thr Leu Arg Asp Gly Ala Wing 170 175 180 acc att gcc tct gaa cta tct gat ccc tcc age acc tac cgc tcc ggc 691 Thr He Wing Ser Glu Leu Ser Asp Pro Ser Ser Thr Tyr Arg Ser Gly 185 190 195 gtg gac tcc gct gtg age gca tcc cag caa cta gca gcc ggc ctg caa 739 Val Asp Ser Ala Val Being Wing Being Gln Gln Leu Wing Wing Gly Leu Gln 200 205 210 acc ctg aaa gac gga tcc age caa ctc age atc ggt gca cgc acc ctc 787 Thr Leu Lys Asp Gly Ser Ser Gln Leu Ser He Gly Wing Arg Thr Leu 215 220 225 gct gat ggc acc age caa ttg gcc gca ggt tcc gaa cag cta gtt gtt 835 Wing Asp Gly Thr Ser Gln Leu Wing Wing Gly Ser Glu Gln Leu Val Val 230 235 240 245 ggc gca caa gca ctg cgc gac ggc acc gtc cag ctt gat gaa ggc tcc 883 Gly Ala Gln Ala Leu Arg Asp Gly Thr Val Gln Leu Asp Glu Gly Ser 250 255 260 age gaa ctc gcc ctc aaa ctc acc gac ggc gca age caa gta cca acc 931 Ser Glu Leu Wing Leu Lys Leu Thr Asp Gly Wing Wing Gln Val Pro Thr 265 270 275 ttc gct gac ggc gca gac acc acc atc gca acc cca gtt gaa aca gaa 979 Phe Wing Asp Gly Wing Asp Thr Thr He Wing Thr Pro Val Glu Thr Glu 280 285 290 caa gca gga gac acc aca ccg ctc ttc ggt att ggt ctc gca cca tcc 1027 Gln Ala Gly Asp Thr Thr Pro Leu Phe Gly He Gly Leu Wing Pro Phe 295 300 305 ttc atg gct gtc ggc ctg ttc atg gga gca acc gtt gcc ggg tgg atg atc 1075 Phe Met Wing Val Gly Leu Phe Met Gly Wing Thr Val Wing Trp Met He 310 315 320 325 ctg cac cca atc agt cgc cgc gca ctc gac tcc cgc atg gga ggc ttc 1123 Leu His Pro He Being Arg Arg Ala Leu Asp Being Arg Met Gly Gly Phe 330 335 340 cg ggc acc ctg gca age tac ctt cca tea aca gtc tta ggc ctt ggc 1171 Arg Gly Thr Leu Wing Ser Tyr Leu Pro Ser Thr Val Leu Gly Leu Gly 345 350 355 caa gca acc atc atg tgg gca gta ctg tac ttc ctg ctc gac ctc aat 1219 Gln Ala Thr He Met Trp Ala Val Leu Tyr Phe Leu Leu Asp Leu Asn ^ H | MHMj | MM 360 365 370 cca gct cac cca gct gga ctg tgg atg gcg atg gtc gcc atc tea tgg 1267 Pro Ala His Pro Wing Gly Leu Trp Met Wing Met Val Wing He Ser Trp 375 380 385 gta ttc atc tcc att acc cat atg ttc aac aac gtg gca gga ccc tcc 1315 Val Phe He Ser He Thr His Met Phe Asn Asn Val Wing Gly Pro Ser 390 395 400 405 gca ggc cgt gtg ctg tcc atc gtg atg tcc tcc cag cta gtc tcc 1363 Wing Gly Arg Val Leu Ser He Val Met Met Ser Phe Gln Leu Val Ser 410 415 420 tcc ggt ggc cta tac cca cca gaa acc cag cca gca ttc ttc cac tgg 1411 Ser Gly Gly Leu Tyr Pro Pro Glu Thr Gln Pro Wing Phe Phe His Trp 425 430 435 ttc cac acc tac gac ccg atc acc tac gca gtc aac ctc gtg cgc caa 1459 Phe His Thr Tyr Asp Pro He Thr Tyr Ala Val Asn Leu Val Arg Gln 10 440 445 450 atc atc tcc aac gaac acccca tcc aac gac ccacgc tcc ata caa gca 1507 Met He Phe Asn Glu Thr Pro Ser Asn Asp Pro Arg Phe He Gln Wing 455 460 465 atc tgg gta ctc ctc ttc atc tgg gca ctg atg ctc gcc atc tcc acc 1555 He Trp Val Leu Leu Phe He Trp Wing Leu Met Leu Wing Ser Thr 470 475 480 485 ctg gcg aat aga aac aag gtt ctt cgc atg aag gac tac cac cca 1603 Leu Ala Asn Arg Thr Asn Lys Val Leu Arg Met Lys Asp Tyr His Pro 15 490 495 500 gaa ctg aag gtc taaaagcttt tcccgcccgg ttc 1638 Glu Leu Lys Val 505 < 210 > 292 < 211 > 505 • < 212 > PRT < 213 > Corynebacterium glutamicum 20 < 400 > 292 Val Thr Ser Asp Ser Pro Wing Pro Wing Thr Val Asn Wing Val Phe Asn 1 5 10 15 Asn Being Asn Gly Phe He Wing Being Met Leu Gly Asn Gln Val Val Asn 20 25 30 Thr Val Val Glu Thr Met Asp Thr Glu Phe Gly Val Arg He Val Asp 35 40 45 Asn Met Leu Val Gly Phe Ser Thr Leu Gly Asp Gly Met Asn Gln Wing 50 55 60 25 gfg jtfj Wing Glu Gly Wing Thr Thr Leu Being Asp Gly Val Gly Being Wing Asn Asp 65 70 75 80 Gly Wing Val Gln Leu Wing Asp Gly Wing Val Thr Leu Arg Asp Gly He 85 90 95 Wing Being Wing Asn Glu Gly Wing Gln Being Leu Ala Asp Gly Ala Ser Gln 100 105. 110 • Leu Asp Thr Gly Leu Gly Be Ala Wing Thr Gly Ser Gln Thr Leu Wing 115 120 125 Asp Gly Leu Ser Ser Leu Ser Wing Gly Thr Wing Gln Leu Gly Gln Gly 130 135 140 Wing Thr Gln Val Ser Asp Gly Val Gly Gln Leu Val Asp Gln Val Wing 145 150 155 160 Pro Leu Thr Wing Tyr Val Pro Asp He Asn Ser Gln Leu He Thr Leu 165 170 175 10 Arg Asp Gly Wing Wing Thr He Wing Ser Glu Leu Ser Asp Pro Ser Ser 180 185 190 Thr Tyr Arg Ser Gly Val Asp Ser Aia Val Ser Ala Ser Gln Gln Leu 195 200 205 Wing Wing Gly Leu Gln Thr Leu Lys Asp Gly Ser Ser Gln Leu Ser He 210 215 220 Gly Wing Arg Thr Leu Wing Asp Gly Thr Ser Gln Leu Wing Wing Gly Ser 225 230 235 240 15 Glu Gln Leu Val Val Gly Ala Gln Ala Leu Arg Asp Gly Thr Val Gln 245 250 255 Leu Asp Glu Gly Be Ser Glu Leu Ala Leu Lys Leu Thr Asp Gly Wing 260 265 270 Be Gln Val Pro Thr Phe Wing Asp Gly Wing Asp Thr Thr He Wing Thr 275 280 285 Pro Val Glu Thr Glu Gln Wing Gly Asp Thr Thr Pro Leu Phe Gly He 290 295 300 20 Gly Leu Wing Pro Phe Phe Met Wing Val Gly Leu Phe Met Gly Wing Thr 305 310 315 320 Val Wing Trp Met He Leu His Pro He Ser Arg Arg Ala Leu Asp Ser 325 330 335 Arg Met Gly Gly Phe Arg Gly Thr Leu Wing Ser Tyr Leu Pro Ser Thr 340 345 350 Val Leu Gly Leu Gly Gln Wing Thr He Met Trp Wing Val Leu Tyr Phe 355 360 365 25 i ii i iiMiM * "*" - '- - •' - * - Leu Leu Asp Leu Asn Pro Wing His Pro Wing Gly Leu Trp Met Wing Met 370 375 380 Val Wing He Ser Trp Val Phe He Ser He Thr His Mer Phe Asn Asn 385 390 395 400 Val Wing Gly Pro Wing Wing Gly Arg Val Leu Ser He Val Met Met Ser 405 410 415 Phe Gln Leu Val Ser Ser Gly Gly Leu Tyr Pro Pro Glu Thr Gln Pro 420 425 430 Wing Phe Phe His Trp Phe His Thr Tyr Asp Pro He Thr Tyr Wing Val 435 440 445 Asn Leu Val Arg Gln Met He Phe Asn Glu Thr Pro Ser Asn Asp Pro 450 455 460 Arg Phe He Gln Wing He Trp Val Leu Leu Phe He Trp Wing Leu Met 465 470 475 480 Leu Wing He Ser Thr Leu Wing Asn Arg Thr Asn Lys Val Leu Arg Met 485 490 495 Lys Asp Tyr His Pro Glu Leu Lys Val 500 505 < 210 > 293 < 211 > 664 < 212 > DNA < 213 > Corynebacterium glutamicum <; 220 > < 221 > CDS < 222 > (101) .. (664) < 223 > RXN01091 < 400 > 293 tcgtcggaaa aactggcgat actattgata acgggcatgg gttcaccttc gataaagcac 60 ggtctatgct ggactatcgc cttttgacac gagtatcgca atg gtc ccg aac aca 115 Met Val Pro Asn Thr 1 5 gtc ctt atc cat gac gaa acc gcc gat ctg gcg acg cag atc cag cgg 163 Val Leu He His Asp Glu Thr Ala Asp Leu Wing Thr Gln He Gln Arg 10 15 20 ctg gaa cat atc atg gcg tgc ctg cgc gat ccg gtc age gga tgc ccg 211 Leu Glu His He Met Wing Cys Leu Arg Asp Pro Val Ser Gly Cys Pro 25 30 35 tgg gat att gaa cag acc ttt gcc age atc gcg ccc cac acg att gag 259 Trp Asp He Glu Gln Thr Phe Wing Be He Wing Pro His Thr He Glu 40 45 50 ^ ^, ^ ___ ^, _ ". - ... yy _ «^ ^ ^ ^ ^ gaa ggc tac gag gtt gcc gac gcc atc gcg cag gaa gac tgg ccc gag 307 Glu Gly Tyr Glu Val Wing Asp Wing He Wing Gln Glu Asp Trp Pro Glu 55 60 65 cta cgc ggc gag ttg ggc gat ttg ctg ttt cag acc gtg ttt cac gcc 355 Leu Arg Gly Glu Leu Gly Asp Leu Leu Phe Gln Thr Val Phe His Wing 70 75 80 85 5 caa atg gcg cgc gag gca ggc cat ttc gct ttg gtt gac gtg gtg aag 403 Gln Met Wing Arg Glu Wing Gly His Phe Wing Leu Val Asp Val Val Lys 90 95 100 gca att tcg gac aag atg gtt ttg cgc cat ccg cac gtg ttc ggc gcg 451 Wing He Be Asp Lys Met Val Leu Arg His Pro His Val Phe Gly Wing 105 110 115 cag tcg aac gcg aaa tcc gcc gac cag gg t ga ga g tg gaa gtc 499 Gln Ser Asn Wing Lys Ser Wing Asp Gln Gln Val Glu Asp Trp Glu Val 120 125 130 10 atc aag gcg ccc gag cgc gcg ggc aaa gcg caa aag ggc gtt ttg gat 547 He Lys Wing Pro Glu Arg Wing Gly Lys Wing Gln Lys Gly Val Leu Asp 135 140 145 ggc gtc gcg gtg gga ctg ect gcc ctg atg cgc gcg acg aag ctg caa 595 Gly Val Wing Leu Gly Leu Pro Wing Leu Met Arg Wing Thr Lys Leu Gln 150 155 160 165 aac aac gcc gcg cgc gtt ggg ttt gat tgg ccc gac att ggg cag gta 643 Asn Asn Ala Ala Arg Val Gly Phe Asp Trp Pro Asp He Gly Gln Val 170 175 180 15 ctt gga aag gtg acc gag gaa 664 Leu Gly Lys Val Thr Glu Glu 185 < 210 > 294 < 211 > 188 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 294 Met Val Pro Asn Thr Val Leu He His Asp Glu Thr Wing Asp Leu Wing 20 1 5 10 15 Thr Gln He Gln Arg Leu Glu His He Met Wing Cys Leu Arg Asp Pro 20 25 30 Val Ser Gly Cys Pro Trp Asp He Glu Gln Thr Phe Wing Ser Wing 35 40 45 Pro His Thr He Glu Glu Gly Tyr Glu Val Wing Asp Wing He Wing Gln 50 55 60 Glu Asp Trp Pro Glu Leu Arg Gly Glu Leu Gly Asp Leu Leu Phe Gln 25 65 70 75 80 Thr Val Phe His Wing Gln Met Avia Arg Glu Wing Gly His Phe Wing Leu 85 90 95 Val Asp Val Val Lys Wing He As Asp Lys Met Val Leu Arg His Pro 100 105 110 His Val Phe Gly Wing Gln Ser Asn Ala Lys Ser Wing Asp Gln Gln Val 115 120 125 Glu Asp Trp Glu Val He Lys Wing Pro Glu Arg Wing Gly Lys Wing Gln 130 135 140 Lys Gly Val Leu Asp Gly Val Wing Leu Gly Leu Pro Wing Leu Met Arg 145 150 155 160 Wing Thr Lys Leu Gln Asn Asn Ala Ala Arg Val Gly Phe Asp Trp Pro 165 170 175 Asp He Gly Gln Val Leu Gly Lys Val Thr Glu Glu 180 185 10 < 210 > 295 < 211 > 357 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (334) < 223 > RXS02979 15 < 400 > 295 ctaggtcaag gaaetteact cggctagtcc ttagactcaa atgtgttcag acaaacactg 60 gcaccgtaag gcacgaaagt taccgaaagg actggttccc atg acc gcc cca aac 115 Met Thr Ala Pro Asn 1 5 act ctc aag caa aca act ctt cgc tct gat gag ttc tct tgc cca tcc 163 Thr Leu Lys Gln Thr Thr Leu Arg Be Asp Glu Phe Ser Cys Pro Ser 10 15 20 20 tgt gtc tcc aag att gaa aac aaa ttg aat gga ttg gat ggc gtc gac 211 Cys Val Ser Lys He Glu Asn Lys Leu Asn Gly Leu Asp Gly Val Asp 25 30 35 aat gca gag gtg aag ttc tcc tcc gga aga atc ctt gtt gat cac gac 259 Asn Wing Glu Val Lys Phe Ser Ser Gly Arg He Leu Val Asp His Asp 40 45 50 ccc age aag gtc tct atc aag gat cta gtc gct gca gtc gca gag gtt 307 Pro Ser Lys Val Ser He Lys Asp Leu Val Wing Wing Val Wing Glu Val 55 60 65 25 ggc tac acc gca aag cca tea gca atc taaaactctc agttagacca 354 i i tr1? íiiiÉMría * MM 'it-- - - • • - • • 1. Hy., «*» Ma Gly Tyr Thr Ala Lys Pro Ser Ala He 70 75 tta 357 < 210 > 296 < 211 > 78 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 296 Met Thr Ala Pro Asn Thr Leu Lys Gln Thr Thr Leu Arg Ser Asp Glu 1 5 10 15 Phe Ser Cys Pro Ser Cys Val Ser Lys He Glu Asn Lys Leu Asn Gly 20 25 30 Leu Asp Gly Val Asp Asn Ala Glu Val Lys Phe Ser Ser Gly Arg He 35 40 45 Leu Val Asp His Asp Pro Ser Lys Val Ser He Lys Asp Leu Val Wing 50 55 60 Wing Val Wing Glu Val Gly Tyr Thr Wing Lys Pro Ser Wing He 65 70 75 < 210 > 297 < 211 > 357 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (334) < 223 > RXS02987 < 400 > 297 gttgtttgat ccaggtcaag gaattaaccc ggaaaggacc gtatctttaa aggtgcaagc 60 acaggaacat gacgataaaa gatgaaagga cctggttacg atg acc gcc ccc gcc 115 Met Thr Wing Pro Wing 1 5 acg ctg aag aac acc acc ttg cgc tct gat gag ttc acc tgt ccg age 163 Thr Leu Lys Asn Thr Thr Leu Arg Ser Asp Glu Phe Thr Cys Pro Ser 10 15 20 tgt gtc gcc aag atc gaa aac aag ctg aat ggt ttg gac ggc gtg gag 211 Cys Val Ala Lys He Glu Asn Lys Leu Asn Gly Leu Asp Gly Val Glu 25 30 35 aat gcg gag gtg aag ttc tcc tcc gga cgc atc ctg atc acc acc gac 259 Asn Ala Glu Val Lys Phe Ser Ser Gly Arg He Leu He Thr His Asp 40 45 50 »., L. .., '? Í-.i.? cca cag aag gtc tcc gta cgt gac ctg gtc acc gcg gta gcc gag gtc 307 Pro Gln Lys Val Ser Val Arg Asp Leu Val Thr Ala Val Ala Glu Val 55 60 65 ggt tac acc gcc aag ccg tcg gcg atc tgacgcactc ccgaccccac 354 Gly Tyr Thr Ala Lys Pro Ser Ala He 70 75 aag 357 < 210 > 298 < 211 > 78 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 298 Met Thr Ala Pro Wing Thr Leu Lys Asn Thr Thr Leu Arg Ser Asp Glu 1 5 10 15 10 Phe Thr Cys Pro Ser Cys Val Wing Lys He Glu Asn Lys Leu Asn Gly 20 25 30 Leu Asp Gly Val Glu Asn Wing Glu Val Lys Phe Ser Ser Gly Arg He 35 40 45 Leu He Thr His Asp Pro Gln Lys Val Ser Val Arg Asp Leu Val Thr 50 55 60 Wing Val Wing Glu Val Gly Tyr Thr Wing Lys Pro Ser Wing He 65 70 75 15 < 210 > 299 < 211 > 492 < 212 > DNA < 213 > Corynebacterium glutamicum < 220 > < 221 > CDS < 222 > (101) .. (469) < 223 > RXS03095 < 400 > 299 20 aacgcctcaa ttagtgccag accttgccga ccgcagacca aacttcacca tttcaaacca 60 tccctagcca caacaacggc agttgtgcaa tgatctgcgt atg aat gca gat aag 115 Met Asn Wing Asp Lys 1 5 aaa atg tgc gga atg aac ccg gat age caa tac gtc gaa ctt gcc gtc 163 Lys Met Cys Gly Met Asn Pro Asp Ser Gln Tyr Val Glu Leu Wing Val 10 15 20 gaa gtt ttc gga ctc ctc gcg gac gcc act cga gtt cgc atc atc ttg 211 Glu Val Phe Gly Leu Leu Wing Asp Wing Thr Arg Val Arg He He Leu 25 25 30 35 gca ctt cga aac agt ggt gaa ctt tcc gta aac cac ctc gcg gac atc 259 Wing Leu Arg Asn Ser Gly Glu Leu Ser Val Asn His Leu Wing Asp He 40 45 50 gtc gat aaa tcc ccc gca gca gtt tcc caa cac ctc gcc cgg ctg cgc 307 Val Asp Lys Ser Pro Wing Wing Val Ser Gln His Leu Wing Arg Leu Arg 55 60 65 atg gcc cga atc gtg tcc acc cgt caa gaa ggt caa cga gtt tcc tac 355 Met Wing Arg He Val Ser Thr Arg Gln Glu Gly Gln Arg Val Phe Tyr 70 75 80 85 aaa ctc acc aat gaa cac gca tea cag cta gtc tcc gac gct att ttt 403 Lys Leu Thr Asn Glu His Ala Ser Gln Leu Val Ser Asp Wing He Phe 90 95 100 cag gcg gaa cac acc att gcg gac ggc cag act ccc cca cac cac cac 451 Gln Ala Glu His Thr He Wing Asp Gly Gln Thr Pro Pro His His His 105 110 115 10 cga gaga cga gaa caa tea tgaccaccca cagtcaccaa gaa 492 Arg Glu Arg Glu Gln Ser 120 < 210 > 300 < 211 > 123 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 300 15 Met Asn Wing Asp Lys Lys Met Cys Gly Met Asn Pro Asp Ser Gln Tyr 1 5 10 15 Val Glu Leu Wing Val Glu Val Phe Gly Leu Leu Wing Asp Wing Thr Arg 20 25 30 Val Arg He He Leu Wing Leu Arg Asn Ser Gly Glu Leu Ser Val Asn 35 40 45 His Leu Wing Asp He Val Asp Lys Ser Pro Wing Wing Val Ser Gln His 50 55 60 20 Leu Wing Arg Leu Arg Met Wing Arg He Val Ser Thr Arg Gln Glu Gly 65 70 75 80 Gln Arg Val Phe Tyr Lys Leu Thr Asn Glu His Wing Ser Gln Leu Val 85 90 95 Ser Asp Wing He Phe Gln Wing Glu His Thr He Wing Asp Gly Gln Thr 100 105 110 Pro Pro His His His Arg Glu Arg Glu Gln Ser 115 120 25 ^ - ^^^ a ^ - - --- '- - ^ - < 210 > 301 < 211 > 10 < 212 > PRT < 213 > Corynebacterium glutamicum < 400 > 301 Wing Pro Wing Leu Gly Pro Thr Leu Ser Gly 1 5 10 < 210 > 302 < 211 > 10 < 212 > PRT < 213 > Corynebacterium glutamicum < 220 > < 221 > VARIANT < 222 > 2-4, 7, 8 < 223 > Xaa = any amino acid < 400 > 302 Gly Xaa Xaa Xaa Gly Pro Xaa Xaa Gly Gly 1 5 10 < 210 > 303 < 211 > 20 < 212 > DNA < 213 > Corynebacterium glutamicum < 400 > 303 ctccaggatt gctccgaagg 20 < 210 > 304 < 211 > 20 < 212 > DNA < 213 > Corynebacterium glutamicum < 400 > 304 cacagtggtt gaccactggc 20 < 210 > 305 < 211 > 18 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of the Artificial Sequence: Primer < 400 > 305 ggaaacagta tgaccatg 18 < 210 > 306 < 211 > 17 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of the Artificial Sequence: Primer < 400 > 306 gtaaaacgac ggccagt 17

Claims

CLAIMS 1. An isolated molecule of Corynebacterium glutamicum nucleic acid that encodes a tolerance or stress resistance gene or a portion thereof, a 5 condition that the nucleic acid molecule does not consist of any of the genes designated with the letter F in Table 1. 2. The isolated nucleic acid molecule of claim 1, wherein said tolerance or stress resistance gene is selected within the group that 10 consists of nucleic acid molecules involved in a stress response, tolerance or resistance to temperature stress, pH stress, oxygen stress, osmotic stress, toxic chemicals, oxygen radicals, antibiotics, or lincomycin. 15 3. A single nucleic acid molecule isolated from Corynebacterium glutamicum which is selected from the group consisting of the sequences presented as SEQ ID NOs of the odd number of the Sequence List, or a portion thereof, provided that the nucleic acid molecule is not 20 consists of none of the genes designated by the letter F presented in Table 1. 4. An isolated nucleic acid molecule encoding a polypeptide sequence that is selected from the group consisting of the sequences presented 25 as SEQ ID NOs of even number of the Sequence List, to : T? A? I ff? MÍi: IIV nV-: r MM ^ B ^ ákáiteta ^ condition that the nucleic acid molecule does not consist of any of the genes designated with the letter F presented in Table 1. 5. A molecule isolated nucleic acid encoding a naturally occurring allelic variant of a polypeptide selected from the group of amino acid sequences consisting of the sequences presented as SEQ ID NOs of even number from the Sequence List, provided that the nucleic acid molecule does not consist of any of the genes designated by the letter F presented in Table 1. 6. An isolated nucleic acid molecule comprising a sequence of nucleotides having a homology level of at least 50% with a nucleotide sequence selected within the group consisting of the sequences presented as SEQ ID NOs of the odd number of the Sequence List or a portion thereof, provided that the nucleic acid molecule is not consists of none of the genes designated by the letter F presented in Table 1. 7. An isolated nucleic acid molecule comprising a fragment of at least 15 nucleotides of a nucleic acid comprising a nucleotide sequence selected from the group consisting of consists of the sequences presented as SEQ ID NOs of the odd number of the Sequence List, provided that the nucleic acid molecule does not consist of any of the genes designated by the letter F presented in Table 1. 8. An isolated molecule of nucleic acid hybridizing with the nucleic acid molecule of any of claims 1-7 under stringent conditions. 9. An isolated nucleic acid molecule comprising the nucleic acid molecule of any of claims 1-8 or a portion thereof and a nucleotide sequence encoding a heterologous polypeptide. 10. A vector comprising the nucleic acid molecule of any of claims 1-9. 11. The vector according to claim 10, which is an expression vector. 12. A host cell transfected with the expression vector of claim 11. 13. The host cell according to claim 12, wherein said cell is a microorganism. 14. The host cell according to claim 13, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. 15. The host cell according to claim 12, wherein the expression of said nucleic acid molecule results in the modulation of the production of a fine chemical from said cell. 16. The host cell according to claim 15Wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and non-proteinogenic amino acids, purine bases and pyrimidine nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins , cofactors, polyketides, and enzymes. 17. A method for producing a polypeptide, comprising culturing the host cell according to claim 121 in an appropriate culture medium to thereby produce the polypeptide. 18. A polypeptide isolated from tolerance or stress resistance of Corynebacterium glutamicum, or a portion thereof. The protein of claim 18, wherein said stress stress tolerance or polypeptide is selected from the group consisting of proteins involved in a stress response, tolerance or resistance to temperature stress, pH stress, stress of oxygen, osmotic stress, toxic chemicals, oxygen radicals, antibiotics, or lincomycin. 20. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of the sequences presented as even number SEQ ID NOs of the Sequence List, provided that the amino acid sequence is not encoded by any of the designated genes with the letter F presented in Table 1. 21. An isolated polypeptide comprising an allelic variant that naturally occurs from a polypeptide comprising an amino acid sequence selected from the group consisting of the sequences presented as SEQ ID NOs of even number of the Sequence List or a portion thereof, provided that the amino acid sequence is not encoded by any of the genes designated by the letter F presented in Table 1. 22. The polypeptide isolated from any of claims 18- 21, which further comprises heterologous amino acid sequences. 23. An isolated polypeptide that is encoded by a nucleic acid molecule comprising a nucleotide sequence having a homology level of at least 50% with a nucleic acid selected from the group consisting of the sequences presented as SEQ ID Odd number NOs of the Sequence List, provided that the nucleic acid molecule does not consist of any of the nucleic acid molecules designated by the letter F presented in Table 1. 24. An isolated polypeptide comprising an amino acid sequence having a homology level of at least 50% with an amino acid sequence selected from the group consisting of the sequences presented as SEQ ID NOs of even number from the Sequence List, provided that the amino acid sequence is not encoded by any of the genes designated by the letter F presented in Table 1. 25. A method for producing a fine chemical, comprising the cultivation of a cell containing a vector of the claim 12, in such a way that the fine chemical is produced. 26. The method according to claim 25, wherein said method comprises the step of recovering the fine chemical from said culture. 27. The method according to claim 25, wherein said method further comprises the step of transfecting said cell with the vector of claim 11 to obtain a cell containing said vector. 28. The method of compliance with the claim 25, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. 29. The method according to claim 25, wherein said cell is selected from the group consisting of: Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus , Brevibacterium ammoniagenes, Brevibacterium butanicum, Brevibacterium divaricatum, Brevibacterium flavum, Brevibacteriumhealii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium linens, Brevibacterium paraffinolyticum, and the strains presented in table 3. The method according to claim 25, wherein the expression of the nucleic acid molecule from said vector results in the modulation of the production of said fine chemical. 31. The method of compliance with the claim 25, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatics, vitamins, cofactors, polyketides and enzymes. 32. The method according to claim 25, wherein said fine chemical is an amino acid. The method according to claim 32, wherein said amino acid is obtained from the group consisting of lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, methionine, cysteine, valine, leucine, isoleucine, Asginine, proline, histidine, tyrosine, phenylalanine and tryptophan. 34. A method for the production of a fine chemical, comprising the cultivation of a cell whose genomic DNA has been altered by the inclusion of a nucleic acid molecule of any of claims 1-9. 35. A method for diagnosing the presence or activity of Corynebacterium diphtheriae in a subject, comprising detecting the presence of one or more of SEQ ID NOs 1 through 304 of the Sequence List in the subject, provided that the sequences are not encoded by any of the sequences designated with the letter FF presented in Table 1, thus diagnosing the presence or activity of Corynebacterium diphtheriae in the subject. 36. A host cell comprising a nucleic acid molecule selected from the group consisting of the nucleic acid molecules presented as SEQ ID NOs of the odd number in the Sequence Listing, wherein the nucleic acid molecule is disorganized. 37. A host cell comprising a nucleic acid molecule selected from the group consisting of the nucleic acid molecules presented as SEQ ID Odd number NOs from the Sequence List, wherein the nucleic acid molecule comprises one or more nucleic acid modifications of the sequence presented as SEQ ID NOs of the odd number of the Sequence List. 38. A host cell comprising a nucleic acid molecule selected from the group consisting of the nucleic acid molecules presented as SEQ ID NOs of the odd number in the Sequence Listing, wherein the regulatory region of the nucleic acid molecule is modified in relation to the wild-type regulatory region of the molecule.