AU2006200801A1 - Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins - Google Patents

Description

P001 Section 29 Regulation 3.2(2)

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins The following statement is a full description of this invention, including the best method of performing it known to us: (1 CORYNEBACTERIUM GLUTAMICUM GENES ENCODING STRESS, SRESISTANCE AND TOLERANCE PROTEINS 1 Related Applications This application claims priority to prior filed U.S. Provisional Patent Application Serial No. 60/141031, filed June 25, 1999, U.S. Provisional Patent Application Serial 00 0No. 60/142692, filed July 1, 1999, and also to U.S. Provisional Patent Application Serial C No. 60/151214, filed August 27, 1999. This application also claims priority to German SPatent Application No. 19930429.7, filed July 1, 1999, German Patent Application No.

C 10 19931413.6, filed July 8, 1999, German Patent Application No. 19931457.8, filed July 8, 1999, German Patent Application No. 19931541.8, filed July 8, 1999, German Patent Application No. 19932209.0, filed July 9, 1999, German Patent Application No.

19932230.9, filed July 9, 1999, German Patent Application No. 19932914.1, filed July 14, 1999, German Patent Application No. 19940764.9, filed August 27, 1999, and German Patent Application No. 19941382.7, filed August 31, 1999. The entire contents of all of the aforementioned applications are hereby expressly incorporated herein in their entirety by this reference.

Background of the Invention Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through large-scale culture of bacteria developed to produce and secrete large quantities of a particular desired molecule. One particularly useful organism for this purpose is Corynebacterium glutamicum, a gram positive, nonpathogenic bacterium. Through strain selection, a fiumber of mutant strains have been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.

SSummary of the Invention The invention provides novel bacterial nucleic acid molecules which have a Cvariety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in C.

glutamicum or related bacteria, the typing or identification of C. glutamicum or related 00 Sbacteria, as reference points for mapping the C. glutamicum genome, and as markers for Ctransformation. These novel nucleic acid molecules encode proteins, referred to herein as stress, resistance and tolerance (SRT) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The SRT nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by fermentation processes. Modulation of the expression of the SRT nucleic acids of the invention, or modification of the sequence of the SRT nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).

The SRT nucleic acids of the invention may also be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C.

glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C glutamicum gene which is unique to this organism, one can ascertain whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium diphtheriae (the causative agent of diphtheria); the detection of such organisms is of significant clinical relevance.

The SRT nucleic acid molecules of the invention may also serve as reference points for mapping of the C. glutamicum genome, or of genomes of related organisms.

-3- N1 Similarly, these molecules, or variants or portions thereof, may serve as markers for Sgenetically engineered Corynebacterium or Brevibacterium species.

The SRT proteins encoded by the novel nucleic acid molecules of the invention

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7, are capable of, for example, permitting C. glutamicum to survive in a setting which is either chemically or environmentally hazardous to this microorganism. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those 00 0disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C. glutamicum and the related Brevibacterium species 0lactofermentum) (Yoshihama et al, J. Bacteriol. 162: 591-597 (1985); Katsumata et al., C 10 J. Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 2237- 2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals, through the ability of these proteins to permit growth and multiplication of C. glutamicum (and also continuous production of one or more fine chemicals) under circumstances which would normally impede growth of the organism, such as those conditions frequently encountered during large-scale fermentative growth.

For example, by overexpressing or engineering a heat-shock induced protease molecule such that it is optimized in activity, one may increase the ability of the bacterium to degrade incorrectly folded proteins when the bacterium is challenged with high temperatures. By having fewer misfolded (and possibly misregulated or nonfunctional) proteins to interfere with normal reaction mechanisms in the cell, the cell is increased in its ability to function normally in such a culture, which should in turn provide increased viability. This overall increase in number of cells having greater viability and activity in the culture should also result in an increase in yield, production, and/or efficiency of production of one or more desired fine chemicals, due at least to the relatively greater number of cells producing these chemicals in the culture.

This invention provides novel SRT nucleic acid molecules which encode SRT proteins which are capable of, for example, permitting C. glutamicum to survive in a setting which is either chemically or environmentally hazardous to this microorganism.

Nucleic acid molecules encoding an SRT protein are referred to herein as SRT nucleic acid molecules. In a preferred embodiment, the SRT protein participates in metabolic pathways permitting C. glutamicum to survive in a setting which is either chemically or environmentally hazardous to this microorganism. Examples of such proteins C) include those encoded by the genes set forth in Table 1.

SThe following embodiments of the invention, the subject of this application, are specifically disclosed herein: C 5 An isolated Corynebacterium glutamicum nucleic acid molecule comprising a nucleic acid sequence selected from the group 0consisting of each of the odd numbered SEQ ID NOs: 3 to 299, o00 0SEQ ID NO: 303 and SEQ ID NO: 304, provided that the nucleic c acid molecule does not consist of any of the F-designated genes set 0 10 forth in Table 1, or a complement thereof.

c An isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

SAn isolated nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 or SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of 0 any of the F-designated genes set forth in Table 1, or a complement 0 thereof.

An isolated polypeptide comprising an amino acid sequence Sselected from the group consisting of each of the even numbered i 5 SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the SF-designated genes set forth in Table 1.

00 0* An isolated polypeptide which is encoded by a nucleic acid 0 N molecule comprising a nucleotide sequence which is at least O 10 identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

An isolated polypeptide comprising an amino acid sequence which is at least 50% identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1..

An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the nucleic acid molecule is disrupted.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SSEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304.

D A host cell comprising a nucleic acid molecule selected from the Sgroup consisting of each of the odd numbered SEQ ID NOs: 3 to c 5 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild- Stype regulatory region of the molecule.

oo 0 Accordingly, one aspect of the invention pertains to isolated nucleic acid C molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence \0 0 10 encoding an SRT protein or biologically active portions thereof, as well as nucleic cN acid fragments suitable as primers or hybridization probes for the detection or amplification of SRT-encoding nucleic acid DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth as an even-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID The preferred SRT proteins of the present invention also preferably possess at least one of the SRT activities described herein.

In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence having an even-numbered SEQ ID NO: in the Sequence Listing), sufficiently homologous to an amino acid sequence of the invention such that the protein or portion thereof maintains an SRT activity.

c- Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to increase the survival of C. glutamicum in a setting which is L either chemically or environmentally hazardous to this microorganism. In one CN 5 embodiment, the protein encoded by the nucleic acid molecule is at least about preferably at least about 60%, and more preferably at least about or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or 00 0more homologous to an amino acid sequence of the invention an entire amino acid Ssequence selected from those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown the corresponding odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID 00 SEQ In another preferred embodiment, the isolated nucleic acid molecule is derived Ifrom C. glutamicum and encodes a protein an SRT fusion protein) which includes 10 a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered SEQ ID NOs in the Sequence Listing) and has the ability to increase the survival of C.

glutamicum in a setting which is either chemically or environmentally hazardous to this.

microorganism, or possesses one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

In another embodiment, the isolated nucleic acid molecule is at least nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an oddnumbered SEQ ID NO in the Sequence Listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C. glutamicum SRT protein, or a biologically active portion thereof.

Another aspect of the invention pertains to vectors, recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which such vectors have been introduced. In one embodiment, such a host cell is used to produce an SRT protein by culturing the host cell in a suitable medium. The SRT protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered microorganism in which an SRT gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by the introduction of a nucleic acid molecule of the invention encoding wild-type or mutated SRT sequence as C a transgene. In another embodiment, an endogenous SRT gene within the genome of the dmicroorganism has been altered, functionally disrupted, by homologous recombination with an altered SRT gene. In another embodiment, an endogenous or

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introduced SRT gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional SRT protein. In still 0 another embodiment, one or more of the regulatory regions a promoter, repressor, 00 0or inducer) of a SRT gene in a microorganism has been altered by deletion, Cr truncation, inversion, or point mutation) such that the expression of the SRT gene is Smodulated. In a preferred embodiment, the microorganism belongs to the genus rC 10 Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 304)) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated SRT protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated SRT protein or portion thereof possesses the ability to increase the survival of C. glutamicum in a setting which is either chemically or environmentally hazardous to this microorganism. In another preferred embodiment, the isolated SRT protein or portion thereof is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to increase the survival of C.

glutamicum in a setting which is either chemically or environmentally hazardous to this microorganism.

The invention also provides an isolated preparation of an SRT protein. In preferred embodiments, the SRT protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein (1N which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded by an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing).). In yet another embodiment, the protein is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous 00 to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated SRT protein comprises an amino acid sequence which is at least about 50% or more homologous to 10 one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and is able to improve the survival rate of C.

glutamicum in a setting which is either chemically or environmentally hazardous to this microorganism, or has one or more of the activities set forth in Table 1.

Alternatively, the isolated SRT protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about 96%, 97%, or 99% or more homologousto a nucleotide sequence of one of the even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred that the preferred forms of SRT proteins also have one or more of the SRT bioactivities described herein.

The SRT polypeptide, or a biologically active portion thereof, can be operatively linked to a non-SRT polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the SRT protein alone. In other preferred embodiments, this fusion protein results in increased yields, production, and/or efficiency of production of a desired fine chemical from C. glutamicum. In particularly preferred embodiments, integration of this fusion protein into a host cell modulates the production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an SRT protein, either by interacting with the protein itself or a substrate or binding partner of the SRT protein, or by modulating the transcription or translation of an SRT nucleic acid molecule of the invention.

-8- N, Another aspect of the invention pertains to a method for producing a fine Schemical. This method involves the culturing of a cell containing a vector directing the expression of an SRT nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an SRT nucleic acid. In another preferred embodiment, this method 00 further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

S 10 Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates SRT protein activity or SRT nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated in resistance to one or more toxic chemicals or in resistance to one or more environmental stresses, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates SRT protein activity can be an agent which stimulates SRT protein activity or SRT nucleic acid expression. Examples of agents which stimulate SRT protein activity or SRT nucleic acid expression include small molecules, active SRT proteins, and nucleic acids encoding SRT proteins that have been introduced into the cell. Examples of agents which inhibit SRT activity or expression include small molecules, and antisense SRT nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant SRT gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine.

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I Detailed Description of the Invention d The present invention provides SRT nucleic acid and protein molecules which are involved in the survival of C. glutamicum upon exposure of this microorganism to C chemical or environmental hazards. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, since these SRT Sproteins provide a means for continued growth and multiplication of C. glutamicum in 00 0 the presence of toxic chemicals or hazardous environmental conditions, such as may be

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N encountered during large-scale fermentative growth. By increasing the growth rate or at 0least maintaining normal growth in the face of poor, if not toxic, conditions, one may C 10 increase the yield, production, and/or efficiency of production of one or more fine chemicals from such a culture, at least due to the relatively greater number of cells producing the fine chemical in the culture. Aspects of the invention are further explicated below.

I. Fine Chemicals The term 'fine chemical' is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in N Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: S0818805086 and references therein. The metabolism and uses of certain of these fine Schemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are O essential for normal cellular functioning in all organisms. The term "amino acid" is art- Srecognized. The proteinogenic amino acids, of which there are 20 species, serve as Sc structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in proteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though Lamino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids have been well characterized in both prokaryotic and eukaryotic cells (see, for example, Stryer, L. Biochemistry, 3 rd edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways.

to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also ofmonogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in -11- N both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ Lmethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids Stechnical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, C chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: SWeinheim, 1985.

SThe biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination of aketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a threestep process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain P-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis of prephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production ofhistidine from 5-phosphoribosyl-l-pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of -12c1 the cell (for review see Stryer, L. Biochemistry 3 rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in Sterms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own 00 production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount 10 of that amino acid present in the cell.

B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although they are readily synthesized by other organisms, such as bacteria. These molecules are either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is artrecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot.synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

13 CN The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) C Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological 00 SAssociations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

SThiamin (vitamin Bi) is produced by the chemical coupling of pyrimidine and .I 10 thiazole moieties. Riboflavin (vitamin B 2 is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)-P-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to Palanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in enzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, panthenol (provitamin Bs), pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives -14-

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of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6- Smethylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and p- C1 amino-benzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as the cobalamines and particularly vitamin B 12 and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

00 SThe biosynthesis of vitamin B 1 2 is sufficiently complex that it has not yet been C completely characterized, but many of the enzymes and substrates involved are now

ID

0known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed 'niacin'. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.

The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by largescale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and biotin. Only Vitamin BI 2 is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are CN nucleotides which do not form nucleic acid molecules, but rather serve as energy stores AMP) or as coenzymes FAD and NAD).

Several publications have described the use of these chemicals for these medical C indications, by influencing purine and/or pyrimidine metabolism Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med. Res. Reviews 10: 505-548). Studies of 00 0enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or Santi-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

10 Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- 612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from in a series of steps through the intermediate compound phosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or adenosine-5'-monophosphate (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

-16- C Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) dfrom ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate (CTP).

The deoxy- forms of all of these nucleotides are produced in a one step reduction C reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

00 D. Trehalose Metabolism and Uses

\Q

STrehalose consists of two glucose molecules, bound in a, a-1,1 linkage. It is 10 commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- 467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.

II. Resistance to Damage from Chemicals, Environmental Stress, and Antibiotics Production of fine chemicals is typically performed by large-scale culture of bacteria developed to produce and secrete large quantities of these molecules. However, this type of large-scale fermentation results in the subjection of the microorganisms to stresses of various kinds. These stresses include environmental stress and chemical stress.

A. Resistance to Environmental Stress Examples of environmental stresses typically encountered in large-scale fermentative culture include mechanical stress, heat stress, stress due to limited oxygen, stress due to oxygen radicals, pH stress, and osmotic stress. The stirring mechanism used in most large-scale fermentors to ensure aeration of the culture produces heat, thus increasing the temperature of the culture. Increases in temperature induce the wellcharacterized heat shock response, in which a set of proteins are expressed which not -17-

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I only aid in the survival of the bacterium in the face of high temperatures, but also Sincrease survival in response to a number of other environmental stresses (see Neidhardt, et al., eds. (1996) E. coli and Salmonella. ASM Press: Washington, ri p. 1382-1399; Wosten, M. M. (1998) FEMS Microbiology Reviews 22(3): 127-50; Bahl, H. et al. (1995) FEMS Microbiology Reviews 17(3): 341-348; Zimmerman, J.L., Cohill, P.R. (1991) New Biologist 641-650; Samali, and Orrenius, S. (1998) 00 SCell. Stress Chaperones 228-236, and references contained therein from each of these citations). Regulation of the heat shock response in bacteria is facilitated by specific sigma factors and other cellular regulators of gene expression (Hecker, M., Volker, U (1998). Molecular Microbiology 29(5): 1129-1136). One of the largest problems that the cell encounters when exposed to high temperature is that protein folding is impaired; nascent proteins have sufficient kinetic energy in high temperature circumstances that it is difficult for the growing polypeptide chain to remain in a stable conformation long enough to fold properly. Thus, two of the key types of proteins expressed during the heat shock response consist of chaperones (proteins which assist in the folding or unfolding of other proteins see, Fink, A.L. (1999) Physiol. Rev.

79(2): 425-449), and proteases, which can destroy any improperly folded proteins.

Examples of chaperones expressed during the heat shock response include GroEL and DNAK; proteases known to be expressed during this cellular reaction to heat shock include Lon, FtsH, and CIpB.

Other environmental stresses besides heat may also provoke a stress response.

Though the fermentor stirring process is meant to introduce oxygen into the culture, oxygen may remain in limited supply, particularly when the culture is advanced in growth and the oxygen needs of the culture are thereby increased; an insufficient supply of oxygen is another stress for the microorganism. Cells in fermentor cultures are also subjected to a number of osmotic stresses, particularly when nutrients are added to the culture, resulting in a high extracellular and low intracellular concentration of these molecules. Further, the large quantities of the desired molecules produced by these organisms in culture may contribute to osmotic stress of the bacteria. Lastly, aerobic metabolism such as that used by C. glutamicum results in carbon dioxide as a waste product; secretion of this molecule may acidify the culture medium due to conversion of this molecule to carboxylic acid. Thus, bacteria in culture are also frequently subjected -18- (1,I to acidic pH stress. The converse may also be true when high levels of basic waste Smolecules such as ammonium are present in the culture medium, the bacteria in culture may be subjected to basic pH stress as well.

C To combat such environmental stresses, bacteria have elegant gene systems which are expressed upon exposure to one or more stresses, such as the aforementioned heat shock system. Genes expressed in response to osmotic stress, for example, encode 00 proteins capable of transporting or synthesizing compatible solutes such that osmotic intake or export of a particular molecule is slowed to manageable levels. Other examples of stress-induced bacterial proteins are those involved in trehalose biosynthesis, those 10 encoding enzymes involved in ppGpp metabolism, those involved in signal transduction, particularly those encoding two-component systems which are sensitive to osmotic pressure, and those encoding transcription factors which are responsive to a variety of stress factors RssB analogues and/or sigma factors). Many other such genes and their protein products are known in the art.

B. Resistance to Chemical Stress SAside from environmental stresses, cells may also experience a number of chemical stresses. These may fall into two categories. The first are natural waste products of metabolism and other cellular processes which are secreted by the cell to the surrounding medium. The second are chemicals present in the extracellular medium which do not originate from the cell. Generally, when cells excrete toxic waste products from the concentrated intracellular cytoplasm into the relatively much more dilute extracellular medium, these products dissipate such that extracellular levels of the possibly toxic compound are quite low. However, in large-scale fermentative culture of the bacterium, this may not be the case: so many bacteria are grown in a relatively small environment and at such a high metabolic rate that waste products may accumulate in the medium to nearly toxic levels. Examples of such wastes are carbon dioxide, metal ions, and reactive oxygen species such as hydrogen peroxide. These compounds may interfere with the activity or structure of cell surface molecules, or may re-enter the cell, where they can seriously damage proteins and nucleic acids alike. Certain other chemicals hazardous to the normal functioning of cells may be naturally found in the extracellular medium. For example, metal ions such as mercury, cadmium, nickel or -19- C1 copper are frequently found in water sources, and may form tight complexes with Scellular enzymes which prevent the normal functioning of these proteins.

C1 C. Resistance to Antibiotics Bacteriocidal proteins or antibiotics, may also be found in the extracellular milieu, either through the intervention of the researcher, or as a natural product from oO Sanother organism, utilized to gain a competitive advantage. Microorganisms have several art-known mechanisms to protect themselves against antimicrobial chemicals.

SDegradation, modification, and export of compounds toxic to the cell are common 1 10 methods by which microorganisms eliminate or detoxify antibiotics. Cytoplasmic 'efflux-pumps' are known in several prokaryotes and show similarities to the so-called 'multidrug resistance' proteins from higher eukaryotes (Neyfakh, A. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 4781-4785). Examples of such proteins include emrAB from E. coli (Lomovskaya, 0. and K. Lewis (1992) Proc. Natl. Acad. Sci. USA 89: 8938-8942), ImrB from B. subtilis (Kumano, M. et al. (1997) Microbiology 143: 2775- 2782), smr from S. aureus (Grinius, L.G. et al. (1992) Plasmid 27: 119-129) or cmr from C. glutamicum (Kaidoh, K. et al. (1997) Micro. Drug Resist. 3: 345-350). .C.

glutamicum itself is non-pathogenic, in contrast to several other members of the genus Corynebacterium, such as C. diphtheriae or C. pseudotuberculosis. Several pathogenic Corynebacteria are known to have multiple resistances against a variety of antibiotics, such as C. jeikeium and C. urealyticum (Soriano, F. et al. (1995) Antimicrob. Agents Chemother. 39: 208-214).

Lincosamides are recognized as effective antibiotics against Corynebacterium species (Soriano, F. et al. (1995) Antimicrob. Agents Chemother. 39: 208-214). An unexpected result of the present invention was the identification of a gene encoding a lincosamide-resistance protein (in particular, a lincomycin-resistance protein). The LMRB protein from C. glutamicum shows 40% homology to the product of the ImrB gene from B. subtilis (see Genbank accession no. AL009126), as calculated using version 1.7 of the program CLUSTALW (Thompson, Higgins, Gibson, T. J.

(1994) Nucl. Acids Res. 22: 4673-4680) using standard parameters (PAIRWISE ALIGNMENT PARAMETERS: slow/accurate alignments: Gap Open Penalty 10.00, Gap Extension Penalty 0.10, Protein weight matrix BLOSUM 30, DNA weight (1N matrix IUB, Fast/Approximate alignments: Gap penalty 3, K-tuple (word) size 1, d)No. of top diagonals 5, Window size 5, Toggle Slow/Fast pairwise alignments slow. Multiple alignment parameters: Gap Opening Penalty 10.00, Gap Extension C Penalty 0.05, Delay divergent sequences 40%, DNA transitions weight 0.50, Protein weight matrix BLOSUM series, DNA weight matrix IUB, Use negative matrix OFF).

00 Environmental stress, chemical stress, and antibiotic or other antimicrobial stress may influence the behavior of the microorganisms during fermentor culture, and Imay have an impact on the production of the desired compound from these organisms.

10 For example, osmotic stress of a microorganism may cause inappropriate or inappropriately rapid uptake of one or more compounds which can ultimately lead to cellular damage or death due to osmotic shock. Similarly, chemicals present in the culture, either exogenously added antimicrobial compounds intended to eliminate unwanted microbes) or generated by the bacteria themselves waste compounds such as heavy metals or oxygen radicals, or even antimicrobial compounds) may result in inhibition of fine chemical production or even death of the organism. The genes of' the invention encode C. glutamicum proteins which act to prevent cell damage or death, by specifically counteracting the source or effect of the environmental or chemical stress.

III. Elements and Methods of the Invention The present invention is based, at least in part, on the discovery of novel molecules, referred to herein as SRT nucleic acid and protein molecules, which increase the ability of C. glutamicum to survive in chemically or environmentally hazardous settings. In one embodiment, the SRT molecules function to confer resistance to one or more environmental or chemical stresses to C. glutamicum. In a preferred embodiment, the activity of the SRT molecules of the present invention has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the SRT molecules of the invention are modulated in activity, such that the yield, production, and/or efficiency of production of one or more fine chemicals from C.

glutamicum is also modulated.

-21r, The language, "SRT protein" or "SRT polypeptide" includes proteins which Sparticipate in the resistance of C. glutamicum to one or more environmental or chemical stresses. Examples of SRT proteins include those encoded by the SRT genes set forth in Table 1 and by the odd-numbered SEQ ID NOs. The terms "SRT gene" or "SRT nucleic acid sequence" include nucleic acid sequences encoding an SRT protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence 00 regions. Examples of SRT genes include those set forth in Table 1. The terms \O "production" or "productivity" are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given 10 time and a given fermentation volume kg product per hour per liter). The term "efficiency of production" includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term "yield" or "product/carbon yield" is art-recognized and includes the efficiency of the conversion of the carbon source into the product fine chemical). This is generally written as, for example, kg product per kg carbon source. By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a "biosynthetic pathway" are art-recognized and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms "degradation" or a "degradation pathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language "metabolism" is art-recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound. The terms "resistance" and "tolerance" are art-known and include the ability of a cell to not be affected by exposure to a chemical or an environment which would otherwise be detrimental to the normal functioning of these organisms. The terms "stress" or "hazard" include factors which are detrimental to the normal functioning of -22- N1 cells such as C glutamicum. Examples of stresses include "chemical stress", in which a Scell is exposed to one or more chemicals which are detrimental to the cell, and "environmental stress" where a cell is exposed to an environmental condition outside of C, those to which it is adapted. Chemical stresses may be either natural metabolic waste products such as, but not limited to reactive oxygen species or carbon dioxide, or chemicals otherwise present in the environment, including, but not limited to heavy 00 Smetal ions or bacteriocidal proteins such as antibiotics. Environmental stresses may be, N, but are not limited to temperatures outside of the normal range, suboptimal oxygen Savailability, osmotic pressures, or extremes of pH, for example.

10 In another embodiment, the SRT molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C. glutamicum. Using recombinant genetic techniques, one or more of the SRT proteins of the invention may be manipulated such that its function is modulated. The alteration of activity of stress response, resistance or tolerance genes such that the cell is increased in tolerance to one or more stresses may improve the ability of that cell to grow and multiply in the relatively stressful conditions of largescale fermentor culture. For example, by overexpressing or engineering a heat-shock induced chaperone molecule such that it is optimized in activity, one may increase the ability of the bacterium to correctly fold proteins in the face of nonoptimal temperature conditions. By having fewer misfolded (and possibly misregulated or nonfunctional) proteins, the cell is increased in its ability to function normally in such a culture, which should in turn provide increased viability. This overall increase in number of cells having greater viability and activity in the culture should also result in an increase in the yield, production, and/or efficiency of production of one or more desired fine chemicals, due at least to the relatively greater number of cells producing these chemicals in the culture.

The isolated nucleic acid sequences of the invention are contained within the genome of a Corynebacterium glutamicum strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated C. glutamicum SRT DNAs and the predicted amino acid sequences of the C.

glutamicum SRT proteins are shown the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively.,.

-23- "11 Computational analyses were performed which classified and/or identified these Snucleotide sequences as sequences which encode chemical and environmental stress, resistance, and tolerance proteins.

SThe present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention the sequence of an even-numbered SEQ ID NO of the Sequence Listing). As used 00 herein, a protein which has an amino acid sequence which is substantially homologous Sto a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, the entire selected amino acid sequence. A protein which has an' amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

Ranges and identity values intermediate to the above-recited values, 75%-80% identical, 85-87% identical, 91-92% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included.

The SRT proteins or biologically active portions or fragments thereof of the invention can confer resistance or tolerance to one or more chemical or environmental stresses, or may have one or more of the activities set forth in Table 1.

Various aspects of the invention are described in further detail in the following subsections: A. Isolated Nucleic Acid Molecules One aspect of the invention pertains to isolated nucleic acid molecules that encode SRT polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of SRT-encoding nucleic acid SRT DNA). As used herein, the term "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides -24c 1 of sequence upstream from the 5' end of the coding region and at least about Snucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic 0 acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank 00 0 the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the 0 CN genomic DNA of the organism from which the nucleic acid is derived. For example, in Svarious embodiments, the isolated SRT nucleic acid molecule can contain less than C1 10 about 5 kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived (e.g a C. glutamicum cell). Moreover, an "isolated" nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, a C. glutamicum SRT DNA can be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

Moreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this sequence a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Sequence Listing) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate r' extraction procedure of Chirgwin et al. (1979) Biochemistry 18: 5294-5299) and DNA Dcan be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the 0 nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention 00 Scan be amplified using cDNA or, alternatively, genomic DNA, as a template and C appropriate oligonucleotide primers according to standard PCR amplification Stechniques. The nucleic acid so amplified can be cloned into an appropriate vector and CN 10 characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an SRT nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic acid sequences of the invention, as set forth in the Sequence Listing, correspond to the Corynebacterium glutamicum SRT DNAs of the invention. This DNA comprises sequences encoding SRT proteins the "coding region', indicated in each oddnumbered SEQ ID NO: sequence in the Sequence Listing), as well as 5' untranslated sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID NO: in the Sequence Listing. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the nucleic acid sequences of the Sequence Listing.

For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, RXN, or RXS number having the designation "RXA", "RXN", or "RXS" followed by digits RXA01524, RXN00493, or RXS01027). Each of the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, or RXS designation to eliminate confusion. The recitation "one of the odd-numbered sequences of the Sequence Listing", then, refers to any of the nucleic acid sequences in the Sequence Listing,, which may be also be distinguished by their differing RXA, RXN, or RXS designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also et forth in the Sequence Listing, as an -26- CN even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence. For example, the coding region for RXA01524 is set forth in SEQ ID NO:1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The ,1 sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, or RXS designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequence designated 00 SRXAO 1524 is a translation of the coding region of the nucleotide sequence of nucleic C, acid molecule RXA01524, the amino acid sequence designated RXN00034 is a Stranslation of the coding region of the nucleotide sequence of nucleic acid molecule 10 RXN00034, and the amino acid sequence in designated RXS00568 is a translation of the coding region of the nucleotide sequence of nucleic acid molecule RXS00568. The correspondence between the RXA, RXN, and RXS nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in Table 1.

Several of the genes of the invention are "F-designated genes". An F-designated gene includes those genes set forth in Table 1 which have an in front of the RXA, RXN, or RXS designation. For example, SEQ ID NO:7, designated, as indicated on Table 1, as "F RXA00498", is an F-designated gene, as are SEQ ID NOs: 25, 33, and 37 (designated on Table 1 as "F RXA01345", "F RXA02543", and "F RXA02282", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not intended to include those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, et al. (1998) J. Bacteriol. 180(12): 3159- 3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing, or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences of the invention is one which is sufficiently complementary to one of the nucleotide sequences shown in the Sequence -27- ,11 Listing the sequence of an odd-numbered SEQ ID NO:) such that it can hybridize Sto one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the CK, invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or more preferably at least about 71%, 72%, 00 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the I 10 invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to one of the nucleotide sequences of the invention,, or a portion thereof.

Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an SRT protein. The nucleotide sequences determined from the cloning of the SRT genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning SRT homologues in other cell types and organisms, as well as SRT homologues from other Corynebacteria or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, preferably about more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the oddnumbered SEQ ID NOs of the Sequence Listing),, an anti-sense sequence of one of these sequences, or naturally occurring mutants thereof. Primers based on a nucleotide sequence of the invention can be used in PCR reactions to clone SRT homologues.

-28- (N4 Probes based on the SRT nucleotide sequences can be used to detect transcripts or Sgenomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g. the label C group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme cofactor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an SRT protein, such as by measuring a level of an SRT-encoding 00 nucleic acid in a sample of cells, detecting SRT mRNA levels or determining whether a genomic SRT gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein 10 or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an evennumbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to confer resistance or tolerance of C. glutamicum to one or more chemical or environmental stresses. As used herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side. chain as an amino acid residue in a sequence of one of the evennumbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the protein or portion thereof is capable of participating in the resistance of C. glutamicum to one or more chemical or environmental stresses. Protein members of such metabolic pathways, as described herein, function to increase the resistance or tolerance of C. glutamicum to one or more environmental or chemical hazards or stresses. Examples of such activities are also described herein. Thus, "the function of an SRT protein" contributes to the overall resistance of C. glutamicum to elements of its surroundings which may impede its normal growth or functioning, and/or contributes, either directly or indirectly, to the yield, production, and/or efficiency of production of one or more fine chemicals.

Examples of SRT protein activities are set forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of -29the Sequence Listing). Ranges and identity values intermediate to the above-recited Svalues, 75%-80% identical, 85-87% identical, or 91-92% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included.

Portions of proteins encoded by the SRT nucleic acid molecules of the invention 00 are preferably biologically active portions of one of the SRT proteins. As used herein, the term "biologically active portion of an SRT protein" is intended to include a portion, a domain/motif, of an SRT protein that is capable of imparting resistance or 10 tolerance to one or more environmental or chemical stresses or hazards, or has an activity as set forth in Table 1. To determine whether an SRT protein or a biologically active portion thereof can increase the resistance or tolerance of C. glutamicum to one or more chemical or environmental stresses or hazards, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an SRT protein can be prepared by isolating a portion of one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the SRT protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the SRT protein or peptide.

The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic code and thus encode the same SRT protein as that encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further embodiment, the nucleic acid molecule of the invention encodes a full length C.

glutamicum protein which is substantially homologous to an amino acid sequence of the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

cN It will be understood by one of ordinary skill in the art that in one embodiment Sthe sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to.

Ci the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank 00 Ssequence (or the protein encoded by such a sequence) set forth in Tables 2 or For I example, the invention includes a nucleotide sequence which is greater than and/or at Sleast 39% identical to the nucleotide sequence designated RXA00084 (SEQ ID 10 NO: 189), a nucleotide sequence which is greater than and/or at least 56% identical to the nucleotide sequence designated RXA00605 (SEQ ID NO: 11), and a nucleotide sequence which is greater than and/or at least 50% identical to the nucleotide sequence designated RXA00886 (SEQ ID NO:39). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the .highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C. glutamicum SRT nucleotide sequences set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by one of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of SRT proteins may exist within a population the C.

glutamicum population). Such genetic polymorphism in the SRT gene may exist among individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an SRT protein, preferably a C. glutamicum SRT protein. Such -31 N1 natural variations can typically result in 1-5% variance in the nucleotide sequence of the SRT gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in SRT that are the result of natural variation and that do not alter the C1 functional activity of SRT proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C. glutamicum homologues of the C. glutamicum SRT DNA of the invention can be isolated based on 00 0 their homology to the C. glutamicum SRT nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in 10 another embodiment, an isolated nucleic acid molecule of the invention is at least nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of an odd-numbered SEQ ID NO: of the Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least.60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those of ordinary skill in the art in the art and can be found in Ausubel et al., Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6. A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-65 0 C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature encodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

glutamicum SRT protein.

In addition to naturally-occurring variants of the SRT sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be -32- (1N introduced by mutation into a nucleotide sequence of the invention, thereby leading to Schanges in the amino acid sequence of the encoded SRT protein, without altering the functional ability of the SRT protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the SRT proteins an 00 0even-numbered SEQ ID NO: of the Sequence Listing) without altering the activity of said SRT protein, whereas an "essential" amino acid residue is required for SRT protein Iactivity. Other amino acid residues, however, those that are not conserved or only 10 semi-conserved in the domain having SRT activity) may not be essential for activity and thus are likely to be amenable to alteration without altering SRT activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding SRT proteins that contain changes in amino acid residues that are not essential for SRT activity. Such SRT proteins differ in amino acid sequence from a sequence of an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the SRT activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the invention and is capable of increasing the resistance or tolerance of C. glutamicum to one or more environmental or chemical stresses, or has one or more of the activities set forth in Table 1. Preferably, the protein encoded by the nucleic acid molecule is at least about 50-60% homologous to the amino acid sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about 60-70% homologous to one of these sequences, even more preferably at least about 70-80%, 80-90%, 90-95% homologous to one of these sequences in, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of the invention.

To determine the percent homology of two amino acid sequences one of the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one -33- N sequence one of the amino acid sequences of the invention) is occupied by the Dsame amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of the amino acid sequence), then the molecules are

C

homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between 0 the two sequences is a function of the number of identical positions shared by the 00 0 sequences homology of identical positions/total of positions x 100).

C An isolated nucleic acid molecule encoding an SRT protein homologous to a 0protein sequence of the invention a sequence of an even-numbered SEQ ID NO: of CI 10 the Sequence Listing)can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic acid), uncharged polar side chains glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains threonine, valine, isoleucine) and aromatic side chains tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in an SRT protein is preferably replaced with another amino acid residue from the same side chain family. Altematively, in another embodiment, mutations can be introduced randomly along all or part of an SRT coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for an SRT activity described herein to identify mutants that retain SRT activity. Following mutagenesis of one the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, the encoded protein can be expressed recombinantly and the activity of the protein can -34r" be determined using, for example, assays described herein (see Example 8 of the DExemplification).

In addition to the nucleic acid molecules encoding SRT proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense" nucleic acid comprises a nucleotide sequence 0 which is complementary to a "sense" nucleic acid encoding a protein, e.g., 00 0 complementary to the coding strand of a double-stranded DNA molecule or 0 CN complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can 0hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be C, 10 complementary to an entire SRT coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an SRT protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues the entire coding region of SEQ ID NO.: 120 (RXA00600) comprises nucleotides 1 to1098). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding SRT. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding SRT disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of SRT mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of SRT mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of SRT mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40,45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to CN increase the biological stability of the molecules or to increase the physical stability of Sthe duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified C nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2- 00 thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D- C galactosylqueosine, inosine, N6-isopentenyladenine, -methylguanine, 1-methylinosine, S2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 'I 10 methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an SRT protein to thereby inhibit expression of the protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in -36- Nl which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention C1 is an a-anomeric nucleic acid molecule. An ac-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual p-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

00 SRes. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-omethylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a I chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

I 10 In still another embodiment, an antisense.nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave SRT mRNA transcripts to thereby inhibit translation of SRT mRNA.

A ribozyme having specificity for an SRT-encoding nucleic acid can be designed based upon the nucleotide sequence of an SRT cDNA disclosed herein SEQ ID NO:1 19 (RXA00600)). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an SRT-encoding mRNA. See, Cech et al.

U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No. 5,116,742. Alternatively, SRT mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

Alternatively, SRT gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of an SRT nucleotide sequence an SRT promoter and/or enhancers) to form triple helical structures that prevent transcription of an SRT gene in target cells. See generally, Helene, C. (1991) Anticancer Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N.Y. Acad. Sci. 660:27- 36; and Maher, L.J. (1992) Bioassays 14(12):807-15.

-37- B. Recombinant Expression Vectors and Host Cells SAnother aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an SRT protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA 00 segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial 10 vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form ofplasmids. IA the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector.

However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, -38- Academic Press, San Diego, CA (1990). Regulatory sequences include those which Sdirect constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells.

Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacIl-, T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SPO2, X-PRor X PL, which are used preferably in bacteria. Additional regulatory sequences are, for 00 0 example, promoters from yeasts and fungi, such as ADC1, MFa, AC, P-60, CYC1, C GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, 0usp, STLS1, B33, nos or ubiquitin- or phaseolin-promoters. It is also possible to use C 10 artificial promoters. It will be appreciated by one of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein SRT proteins, mutant forms of SRT proteins, fusion proteins, etc.).

The recombinant expression vectors of the invention can be designed for expression of SRT proteins in prokaryotic or eukaryotic cells. For example, SRT genes can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (see Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefaciens -mediated transformation ofArabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.: 583-586), or mammalian cells.

Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

-39- C<Z Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein Cencoded therein, usually to the amino terminus of the recombinant protein. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification 0 of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion N moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the SRT protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin.

Recombinant SRT protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann etal., (1988) Gene 69:301-315) pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- IIl 113-B1, ,gtl 1, pBdCl, and pET 11d (Studier et al., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89; and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET lid vector relies on transcription from a T7 gnlO-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21 (DE3) or HMS174(DE3) from a resident X prophage harboring a T7 N gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJl01, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming C Streptomyces, while plasmids pUBI 10, pC194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBLl, pSA77, or pAJ667 (Pouwels et al., eds.

00 (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

SOne strategy to maximize recombinant protein expression is to express the N protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as C. glutamicum (Wada et al. (1992) Nucleic Acids Res. 20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.

In another embodiment, the SRT protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, et al., (1987)Embo J. 6:229-234), 2 t, pAG-1, Yep6, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

(1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, the SRT proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf 9 cells) include the pAc series (Smith et al.

(1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

-41 In another embodiment, the SRT proteins of the invention may be expressed in f unicellular plant cells (such as algae) or in plant cells from higher plants the Sspermatophytes, such as crop plants). Examples of plant expression vectors include Sthose detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for 00 plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, 0 pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: ,0 New York IBSN 0 444 904018).

In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC (Kaufman el al. (1987) EMBOJ. 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.

For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissuespecific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) Adv. Immunol. 43:235-275), in particular promoters ofT cell receptors (Winoto and Baltimore (1989) EMBO J. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byre and Ruddle (1989) PNAS 86:5473-5477), pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and -42- (FZ European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) SScience 249:374-379) and the a-fetoprotein promoter (Campes and Tilghman (1989) C" Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense 00 *O orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an SRNA molecule which is antisense to SRT mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which.direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) 1986.

Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an SRT protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those of ordinary skill in the art. Microorganisms related -43- F(A to Corynebacterium glutamicum which may be conveniently used as host cells for the Snucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a 0 0 linearized vector or a gene construct alone without a vector) or nucleic acid in the form Sof a vector a plasmid, phage, phasmid, phagemid, transposon or other DNA)) into N a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAEdextran-mediated transfection, lipofection, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an SRT protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drug selection cells that have incorporated the selectable marker gene will survive, while the other cells die).

To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an SRT gene into which a deletion, addition or substitution has been introduced to thereby alter, functionally disrupt, the SRT gene.

Preferably, this SRT gene is a Corynebacterium glutamicum SRT gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source.

In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous SRT gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, 44- N the vector can be designed such that, upon homologous recombination, the endogenous SRT gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the N endogenous SRT protein). In the homologous recombination vector, the altered portion of the SRT gene is flanked at its 5' and 3' ends by additional nucleic acid of the SRT gene to allow for homologous recombination to occur between the exogenous SRT gene 00 0carried by the vector and an endogenous SRT gene in a microorganism. The additional 0 flanking SRT nucleic acid is of sufficient length for successful homologous Nrecombination with the endogenous gene. Typically, several kilobases of flanking DNA 10 (both at the 5' and 3' ends) are included in the vector (see Thomas, and Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and cells in which the introduced SRT gene has homologously recombined with the endogenous SRT gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene.

For example, inclusion of an SRT gene on a vector placing it under control of the lac operon permits expression of the SRT gene only in the presence of IPTG. Such regulatory systems are well known in the art.

In another embodiment, an endogenous SRT gene in a host cell is disrupted by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced SRT gene in a host cell has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional SRT protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an SRT gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the SRT gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described SRT gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.

F-A A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an SRT protein. Accordingly, the Sinvention further provides methods for producing SRT proteins using the host cells of Sthe invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an SRT protein has been introduced, or into which genome has been introduced a gene encoding a wild-type 0 or altered SRT protein) in a suitable medium until SRT protein is produced. In another embodiment, the method further comprises isolating SRT proteins from the medium or Sthe host cell.

C. Isolated SRT Proteins Another aspect of the invention pertains to isolated SRT proteins, and biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language "substantially free of cellular material" includes preparations of SRT protein in which the protein is separated from cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of SRT protein having less than about 30% (by dry weight) of non-SRT protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-SRT protein, still more preferably less than about 10% of non-SRT protein, and most preferably less than about 5% non-SRT protein. When the SRT protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of SRT protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of SRT protein having less than about 30% (by dry weight) of chemical precursors or non-SRT chemicals, more preferably less than -46 FC( about 20% chemical precursors or non-SRT chemicals, still more preferably less than about 10% chemical precursors or non-SRT chemicals, and most preferably less than Sabout 5% chemical precursors or non-SRT chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the SRT protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum SRT protein in a 00 microorganism such as C. glutamicum.

SAn isolated SRT protein or a portion thereof of the invention can contribute to ID the resistance or tolerance of C. glutamicum to one or more chemical or environmental stresses or hazards, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability to mediate the resistance or tolerance of C. glutamicum to one or more chemical or environmental stresses or hazards. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an SRT protein of the invention has an amino acid sequence set forth as an even-numbered SEQ ID NO: of the Sequence Listing. In yet another preferred embodiment, the SRT protein has an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another preferred embodiment, the SRT protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or.90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper -47iF and/or lower limits are intended to be included. The preferred SRT proteins of the present invention also preferably possess at least one of the SRT activities described 4 herein. For example, a preferred SRT protein of the present invention includes an amino cacid sequence encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention, and which can increase the resistance or tolerance of C. glutamicum to one or more environmental or 00 chemical stresses, or which has one or more of the activities set forth in Table 1.

In other embodiments, the SRT protein is substantially homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and retains the functional activity of the protein of one of the amino acid sequences of the invention yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the SRT protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the SRT activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In another embodiment, the invention pertains to a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

Biologically active portions of an SRT protein include peptides comprising amino acid sequences derived from the amino acid sequence of an SRT protein, an amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing or the amino acid sequence of a protein homologous to an SRT protein, which include fewer amino acids than a full length SRT protein or the full length protein which is homologous to an SRT protein, and exhibit at least one activity of an SRT protein.

-48- F< Typically, biologically active portions (peptides, peptides which are, for example, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an SRT protein. Moreover, other C biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an SRT protein include 0 0 one or more selected domains/motifs or portions thereof having biological activity.

SSRT proteins are preferably produced by recombinant DNA techniques. For O example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the SRT protein is expressed in the host cell. The SRT protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an SRT protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native SRT protein can be isolated from cells endothelial cells), for example using an anti-SRT antibody, which can be produced by standard techniques utilizing an SRT protein or fragment thereof of this invention.

The invention also provides SRT chimeric or fusion proteins. As used herein, an SRT "chimeric protein" or "fusion protein" comprises an SRT polypeptide operatively linked to a non-SRT polypeptide. An "SRT polypeptide" refers to a polypeptide having an amino acid sequence corresponding to SRT, whereas a "non-SRT polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a protein which is not substantially homologous to the SRT protein, a protein which is different from the SRT protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the SRT polypeptide and the non-SRT polypeptide are fused in-frame to each other. The non- SRT polypeptide can be fused to the N-terminus or C-terminus of the SRT polypeptide.

For example, in one embodiment the fusion protein is a GST-SRT -fusion protein in which the SRT sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant SRT proteins. In another embodiment, the fusion protein is an SRT protein containing a heterologous signal sequence at its N-terminus. In certain host cells mammalian host cells), expression -49- C<1 and/or secretion of an SRT protein can be increased through use of a heterologous signal Ssequence.

SPreferably; an SRT chimeric or fusion protein of the invention is produced by N, standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended 0 0 0 termini for ligation, restriction enzyme digestion to provide for appropriate termini, Sfilling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid NO undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers.

Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide). An SRTencoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the SRT protein.

Homologues of the SRT protein can be generated by mutagenesis, discrete point mutation or truncation of the SRT protein. As used herein, the term "homologue" refers to a variant form of the SRT protein which acts as an agonist or antagonist of the activity of the SRT protein. An agonist of the SRT protein can retain substantially the same, or a subset, of the biological activities of the SRT protein. An antagonist of the SRT protein can inhibit one or more of the activities of the naturally occurring form of the SRT protein, by, for example, competitively binding to a downstream or upstream member of the SRT system which includes the SRT protein. Thus, the C. glutamicum SRT protein and homologues thereof of the present invention may increase the tolerance or resistance of C. glutamicum to one or more chemical or environmental stresses.

In an alternative embodiment, homologues of the SRT protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the SRT protein for SRT protein agonist or antagonist activity. In one embodiment, a variegated library of SRT variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of SRT variants can be produced by, for example, enzymatically ligating a mixture of synthetic Soligonucleotides into gene sequences such that a degenerate set of potential SRT Ssequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of SRT sequences therein.

There are a variety of methods which can be used to produce libraries of potential SRT

O

00 homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a O degenerate gene sequence can be performed in an automatic DNA synthesizer, and the \0 synthetic gene then ligated into an appropriate expression vector. Use of a degenerate 0 10 set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential SRT sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477.

In addition, libraries of fragments of the SRT protein coding can be used to generate a variegated population of SRT fragments for screening and subsequent selection of homologues of an SRT protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an SRT coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with S nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the SRT protein.

Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of SRT homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of -51vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was Sdetected. Recursive ensemble mutagenesis (REM), a new technique which enhances the Sfrequency of functional mutants in the libraries, can be used in combination with the screening assays to identify SRT homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

0 0 In another embodiment, cell based assays can be exploited to analyze a

\O

variegated SRT library, using methods well known in the art.

D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of C. glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

glutamicum sequences of interest; evolutionary studies; determination of SRT protein regions required for function; modulation of an SRT protein activity; modulation of the activity of an SRT pathway; and modulation of cellular production of a desired compound, such as a fine chemical.

The SRT nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof. Also, they may be used to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is nonpathogenic, it is related to pathogenic species, such as Corynebacterium diphtheriae. Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the -52- N body. Degenerative changes brought about by the inhibition of protein synthesis in f these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and Sspleen, result in the systemic pathology of the disease. Diphtheria continues to have Shigh incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990.

O

00 In one embodiment, the invention provides a method of identifying the presence Sor activity of Cornyebacterium diphtheriae in a subject. This method includes detection ,O of one or more of the nucleic acid or amino acid sequences of the invention the S 10 sequences set forth as odd-numbered or even-numbered SEQ ID NOs, respectively, in the Sequence Listing) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject. C. glutamicum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glutamicum are homologous to C. diphtheriae nucleic acid and protein molecules, and can therefore be used to detect C. diphtheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies of C. glutamicum proteins. For example, to identify the region of the genome to which a particular C. glutamicum DNA-binding protein binds, the C. glutamicum genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as Brevibacterium lactofermentum.

The SRT nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The resistance processes in which the molecules of the invention participate are utilized by a wide variety of cells; by comparing the sequences -53 Fof the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed.

SSimilarly, such a comparison permits an assessment of which regions of the sequence Nare conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of

C

00 what the protein can tolerate in terms of mutagenesis without losing function.

SThe genes of the invention, the gene encoding LMRB (SEQ ID NO: 1) or IND other gene of the invention encoding a chemical or environmental resistance or tolerance protein resistance against one or more antibiotics), may be used as genetic markers for the genetic transformation of the transfer of additional genes into or disruption of preexisting genes of) organisms such as C. glutamicum or other bacterial species.

Use of these nucleic acid molecules permits efficient selection of organisms which have incorporated a given transgene cassette a plasmid, phage, phasmid, phagemid, transposon, or other nucleic acid element), based on a trait which permits the survival of the organism in an otherwise hostile or toxic environment in the presence of an antimicrobial compound). By employing one or more of the genes of the invention as genetic markers, the speed and ease with which organisms having desirable transformed traits modulated fine chemical production) are engineered and isolated are improved. While it is advantageous to use the genes of the invention for selection of transformed C. gluzamicum and related bacteria, it is possible, as described herein, to use homologs homologs from other organisms), allelic variants or fragments of the gene retaining desired activity. Furthermore, 5' and 3' regulatory elements of the genes of the invention may be modified as described herein by nucleotide substitution, insertion, deletion, or replacement with a more desirable genetic element) to modulate the transcription of the gene. For example, an LMRB variant in which the nucleotide sequence in the region from -1 to -200 5' to the start codon has been altered to modulate (preferably increase) the transcription and/or translation of LMRB may be employed, as can constructs in which a gene of the invention the LMRB gene (SEQ ID NO: is functionally coupled to one or more regulatory signals inducer or repressor binding sequences) which can be used for modulating gene expression.

-54- C Similarly, more than one copy of a gene (functional or inactivated) of the invention may be employed.

SAn additional application of the genes of the invention the gene encoding LMRB (SEQ ID NO: I) or other drug- or antibiotic-resistance gene) is in the discovery of new antibiotics which are active against Corynebacteria and/or other bacteria. For example, a gene of the invention may be expressed (or overexpressed) in a suitable host Sto generate an organism with increased resistance to one or more drugs or antibiotics (in Sthe case of LMRB, lincosamides in particular, especially lincomycin). This type of IN resistant host can subsequently be used to screen for chemicals with bacteriostatic and/or bacteriocidal activity, such as novel antibiotic compounds. It is possible, in particular, to use the genes of the invention the LMRB gene) to identify new antibiotics which are active against those microorganisms which are already resistant to standard antibiotic compounds.

The invention provides methods for screening molecules which modulate the activity of an SRT protein, either by interacting with the protein itself or a substrate or binding partner of the SRT protein, or by modulating the transcription or translation of,.

SRT nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more SRT proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the SRT protein is assessed.

Manipulation of the SRT nucleic acid molecules of the invention may result in the production of SRT proteins having functional differences from the wild-type SRT proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

The goal of such manipulations is to increase the viability and activity of the cell when the cell is exposed to the environmental and chemical stresses and hazards which frequently accompany large-scale fermentative culture. Thus, by increasing the activity or copy number of a heat-shock-regulated protease, one may increase the ability of the cell to destroy incorrectly folded proteins, which may otherwise interfere with normal cellular functioning (for example, by continuing to bind substrates or cofactors although the protein lacks the activity to act on these molecules appropriately). The same is true for the overexpression or optimization of activity of one or more chaperone molecules N, induced by heat or cold shock. These proteins aid in the correct folding of nascent polypeptide chains, and thus their increased activity or presence should increase the Spercentage of correctly folded proteins in the cell, which in turn should increase the N, overall metabolic efficiency and viability of the cells in culture. The overexpression or optimization of the transporter molecules activated by osmotic shock should result in an increased ability on the part of the cell to maintain intracellular homeostasis, thereby 0 00 increasing the viability of these cells in culture. Similarly, the overproduction or Sincrease in activity by mutagenesis of proteins involved in the development of cellular IDresistance to chemical stresses of various kinds (either by transport of the offending chemical out of the cell or by modification of the chemical to a less hazardous substance) should increase the fitness of the organism in the environment containing the hazardous substance large-scale fermentative culture), and thereby may permit relatively larger numbers of cells to survive in such a culture. The net effect of all of these mutagenesis strategies is to increase the quantity of fine-chemical-producing compounds in the culture, thereby increasing the yield, production, and/or efficiency of production of one or more desired fine chemicals from the culture.

This aforementioned list of mutagenesis strategies for SRT proteins to result in increased yields of a desired compound is not meant to be limiting; variations on these mutagenesis strategies will be readily apparent to one of ordinary skill in the art. By these mechanisms, the nucleic acid and protein molecules of the invention may be utilized to generate C. glutamicum or related strains of bacteria expressing mutated SRT nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any natural product of C. glutamicum, which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of C. glutamicum, but which are produced by a C. glutamicum strain of the invention.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence listing cited throughout this application are hereby incorporated by reference.

2006200801 24 Feb 2006 TABLE 1: Genes Included in the Application Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ IDNO 1 2 RXA01 524 GRO0424 29041 30483 Lincomycine RESISTANCE PROTEIN 3 4 RXA00497 GR00124 52 348 10 KD CHAPERONIN 6 RXN00493 WV0086 14389 16002 60 KD CHAPERONIN 7 8 F RXA00498 GR00124 363 1601 60 KD CHAPERONIN 9 10 RXAO 1217 GR00353 802 203 GENERAL STRESS PROTEIN CTC 11 '12 RXA00605 GROO159 7412 5865 CATALAS E (EC 1. 11. 1.6) 13 14 RXA00404 GR00089 2909 594 CARBON STARVATION PROTEIN A 16 RXN03119 WV0098 86877 87008 SUPEROXIDE DISMUTASE [MN] (EC 1.15.1.1) 17 18 RXN03120 WV0098 87351 87476 SUPEROXIDE DISMUTASE [MN] (EC 1.15.1.1) 19 20 RXN00575 WV0323 14716 15252 PHOSPHINOTHRICIN-RESISTANCE PROTEIN 21 22 F RXA00575 GROO156 2130 1648 PHOSPHINOTHRICIN-RESISTANCE PROTEIN Chaperones Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 23 24RXN01345 26 F RXA01 345 27 28 RXA02541 29 30 RXA02542 31 32 RXN02543 33 34 F RXA02543 36 RXN02280 37 38 F RXA02282 39 40 RXA00886 41 42 RXS00568 43 44 RXN03038 46 RXN03039 47 48 RXN03040 49 50 RXN03051 51 52 RXN03054 53 54 RXN02949 56 RXN02462 57 58 RXN01 559 59 60 RXN00046 61 62 RXN01863 63 64 RXN00833 Cni. NT Start NT Stop Function WV0123 4883 3432 Moleculares chaperon (HSP7O/DnaK family) GR00391 1172 6 Molecular chaperones (HSP7O/DnaK family) GR00726 13657 12473 DNAJ PROTEIN GR00726 14518 13865 GRPE PROTEIN W0057 22031 20178 ONAK PROTEIN GR00725 16375 14522 ONAK PROTEIN W0152 1849 26 TRAP11 GR00659 1145 1480 Molecular chaperone, HSP90 family GR00242 12396 13541 DNAJ PROTEIN W0251 2928 1582 TRIGGER FACTOR W0017 42941 43666 PSI PROTEIN VORLAUFER W0018 2 631 PSI PROTEIN VORLAUFER WV0018 761 1069 PSi PROTEIN VORLAUIFER W0022 2832 3566 PSI PROTEIN VORLAUFER W0026 1906 3486 PSi PROTEIN VORLAUFER W0025 31243 31575 PREPROTEIN TRANSLOKASE SECE UNTEREINHEIT W0124 11932 13749 PREPROTEIN TRANSLOKASE SECA UNTEREINHEIT W0171 7795 5954 PROTEIN-EXPORT MEMBRANE PROTEIN SECD W01l19 5363 6058 Signal Erkennung particle GTPase WV0206 1172 24 /O/C Thioredoxin-Ahnliche oxidloreductase W0180 8039 8533 TH-IOL PEROXIDASE (EC 1.11.1.-) 2006200801 24 Feb 2006 Table 1 (continued) C2n!!2: NT Start NT Stop Nucleic Acid SEQ ID NO 67 69 71 73 77 79 81 83 Amino Acid Identification Code SEQ ID NO 66 RXN01676 68 RXN00380 70 RXN00937 72 RXN02325 74 RXN01837 76 RXN01926 78 RXN02002 80 RXN02736 82 RXS03217 84 F RXAO19I7 Function VV0179 12059 11304 THIOL:DISULFIDE AUSTAUSCH PROTEIN DSBD WV0223 836 216 THIOL:DISULFIDE AUSTAUSCH PROTEIN TLPA WV0079 42335 42706 TH-IOREDOXIN WD0047 5527 6393 THIOREDOXIN WV0320 7103 7879 PEPTIDYL-PROLYL CIS-TRANS ISOMERASE (EC 5.2.1.8) WV0284 1 741 PEPTID KETTE RELEASE FACTOR 3 WO01i1 141 518 PEPTID KETTE RELEASE FACTOR 3 WV0074 13600 14556 PUITATIVES OXPPCYCLE PROTEIN OPCA SMALL COLD-SHOCK PROTEIN GROO549 3465 3665 SMALL COLD-SHOCK PROTEIN Proteins involved in stress responses Nucleic Acid SEQ ID NO 87 89 91 93 97 99 101 103 105 107 109 ill Amino Acid Identification Code SEQ ID NO 8T6 RXA02184 88 RXA0081O0 90 RXA01 674 92 RX.A02431 94 RXA02446 96 RXA02861 98 RXA00981 100 RXN00786 102 RXS01 027 104 RXS01 528 106 RXS01 716 108 RXS01835 110 RXS02497 112 RXS02972 Contig.

GR00641 GROO21 8 GR0D467 GR00708 GR00709 GRI0006 GR00276 WV0321 W0143 WO0050 WV0319 W0143 WV0007 WV031 9 GR00640 GR00646 GR00159 GR00159 GR00646 GR00646 GR00646 GROO245 NT Star NT Slop Function 19628 792 1878 2 11640 551 3388 1680 5761 17276 3259 10575 15609 2763 19248 992 2771 1192 11206 1633 4017 706 6768 16749 2774 10045 16535 2353 NT Stop 6743 6199 1457 1843 5760 6916 8993 3201 COLD SHOCK-LIKE PROTEIN CSPC SMALL COLD-SHOCK PROTEIN PROBABLE HYDROGEN PEROXIDE-INDUCIBLE GENES ACTIVATOR damage-inducible protein P OSMOTICALLY INDUCIBLE PROTEIN C probable metallothionein uO3O8aa Mycobacterium leprae GTP PYROPHOSPHOKINASE (EC 2.7.6.5) LYTB PROTEIN DIADENOSINE 5',5-P,P4-TETRAPHOSP HATE HYDROLASE (EC 3.6.1.17) DIADENOSINE 5,5"-P1,P4-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.17) EXOPOLYPHOSPHATASE (EC 3.6. 1.11) GUANOSINE-3,5'-BIS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) EXOPOLYPHOSPHATASE (EC 3.6. 1.11) EXOPOLYPHOSPHATASE (EC 3.6.1.11) Function ARGININE HYDROXIMATE RESISTANCE PROTEIN ARSENATE REDUCTASE ARSENICAL-RESISTANCE PROTEIN ACR3 ARSENICAL-RESISTANCE PROTEIN ACR3 ARSENICAL-RESISTANCE PROTEIN ACR3 ARSENICAL-RESISTANCE PROTEIN ACR3 BACITRACIN RESISTANCE PROTEIN (PUTATIVE UNDECAPRENOL KINASE) (EC 2.7.1.66) BICYCLOMYCIN RESISTANCE PROTEIN Resistance and tolerance Nucleic Acid SEQ ID NO 113 115 117 119 121 123 125 127 Amino Acid Identification Code SEQ ID NO 11 4 RXA02159 116 RXAO22O1 118 RXA00599 120 .RXA00600 122 RXA02200 124 RXA02202 126 RXA02205 128 RXA00900 NT Start 6231 5837 1843 2940 4651 6278 9871 4052 2006200801 24 Feb 2006 Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 Identification Code RXN00901 F RXA00901 RXA00289 RXN01984 F RXA01984 RXA00109 RXA00109 RXA00996 RXN00829 F RXA00829 F RXA00834 RXA00995 RXN00803 F RXA00803 RXA01407 RXA01408 RXN01922 F RXA01922 RXA02060 RXN01936 F RXA01936 F RXA01937 RXN01010 F RXA01010 RXN03142 F RXA01150 RXN02964 F RXA02116 RXA00858 RXA02305 RXA00084 RXA00843 RXA01052 RXA01053 RXA01054 RXN03123 F RXA00993 RXA01051 RXN01873 F RXA01873 RXN00034 F RXA02273 RXN03075 F RXA02907 RXA00479 RXN03124 F RXA01180 Table 1 (continued) Contig. NT Start NT Stop Function W0140 8581 8168 BICYCLOMYCIN RESISTANCE PROTEIN GR00245 4357 3980 BICYCLOMYCIN RESISTANCE PROTEIN GR00046 3263 4438 CHLORAMPHENICOL RESISTANCE PROTEIN W0056 1515 1811 CHLORAMPHENICOL RESISTANCE PROTEIN GR00574 282 4 CHLORAMPHENICOL RESISTANCE PROTEIN GR00015 1176 565 COPPER RESISTANCE PROTEIN C PRECURSOR GR00015 1176 565 COPPER RESISTANCE PROTEIN C PRECURSOR GR00283 1763 1023 DAUNORUBICIN RESISTANCE ATP-BINDING PROTEIN DRRA W0180 7950 5611 DAUNORUBICIN RESISTANCE PROTEIN GR00224 2 256 DAUNORUBICIN RESISTANCE PROTEIN GR00225 463 2025 DAUNORUBICIN RESISTANCE PROTEIN GR00283 1023 283 DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN W0009 53858 52629 METHYLENOMYCIN A RESISTANCE PROTEIN GR00214 4560 5162 METHYLENOMYCIN A RESISTANCE PROTEIN GR00410 3918 3028 METHYLENOMYCIN A RESISTANCE PROTEIN GR00410 4384 4184 METHYLENOMYCIN A RESISTANCE PROTEIN W0020 2031 3182 METHYLENOMYCIN A RESISTANCE PROTEIN GR00552 3 1109 METHYLENOMYCIN A RESISTANCE PROTEIN GR00626 1 339 MYCINAMICIN-RESISTANCE PROTEIN MYRA W0127 40116 41387 MACROLIDE-EFFLUX PROTEIN GR00555 9796 8975 NICKEL RESISTANCE PROTEIN GR00555 10246 9821 NICKEL RESISTANCE PROTEIN W0209 3776 4894 QUINOLONE RESISTANCE NORA PROTEIN GR00288 774 4 QUINOLONE RESISTANCE NORA PROTEIN W0136 5754 4612 QUINOLONE RESISTANCE NORA PROTEIN GR00323 3807 2917 QUINOLONE RESISTANCE NORA PROTEIN W0102 7931 6714 QUINOLONE RESISTANCE NORA PROTEIN GR00636 911 6 QUINOLONE RESISTANCE NORA PROTEIN GR00233 1680 2147 TELLURIUM RESISTANCE PROTEIN TERC GR00663 2921 2070 DAUNOMYCIN C-14 HYDROXYLASE GR00013 2367 1543 VIBRIOBACTIN UTILIZATION PROTEIN VIUB GR00228 3236 3580 ARSENATE REDUCTASE GR00296 3398 3706 MERCURIC REDUCTASE (EC 1.16.1.1) GR00296 3772 4191 MERCURIC REDUCTASE (EC 1.16.1.1) GR00296 4229 4717 MERCURIC REDUCTASE (EC 1.16.1.1) W0106 808 1245 HEAVY METAL TOLERANCE PROTEIN PRECURSOR GR00282 641 6 HEAVY METAL TOLERANCE PROTEIN PRECURSOR GR00296 3298 2690 VANZ PROTEIN, teicoplanin resistance protein W0248 2054 819 Hypothetical Drug Resistance Protein GR00535 855 1946 Hypothetical Drug Resistance Protein W0020 16933 18381 MULTIDRUG RESISTANCE PROTEIN B GR00655 8058 9002 Hypothetical Drug Resistance Protein W0042 2491 3216 Hypothetical Drug Transporter GR10044 1395 2120 Hypothetical Drug Transporter GR00119 16290 14101 Hypothetical Drug Transporter W0108 4 963 Hypothetical Drug Transporter GR00336 4 765 Hypothetical Drug Transporter 2006200801 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 223 224 RXA02586 GR00741 10296 10027 Hypothetical Drug Transporter 225 226 RXA02587 GR00741 12343 10253 Hypothetical Drug Transporter 227 228 RXN03042 W0018 2440 1835 Hypothetical Drug Transporter 229 230 F RXA02893 GR10035 1841 1236 Hypothetical Drug Transporter 231 232 RXA01616 GR00450 1684 203 MULTIDRUG EFFLUX PROTEIN QACB 233 234 RXA01666 GR00463 2307 3683 MULTIDRUG RESISTANCE PROTEIN 235 236 RXA00062 GR00009 13252 11855 MULTIDRUG RESISTANCE PROTEIN B 237 238 RXA00215 GR00032 13834 15294 MULTIDRUG RESISTANCE PROTEIN B 239 240 RXN03064 W0038 4892 6223 MULTIDRUG RESISTANCE PROTEIN B 241 242 F RXA00565 GR00151 4892 5884 MULTIDRUG RESISTANCE PROTEIN B 243 244 F RXA02878 GR10016 1837 1481 MULTIDRUG RESISTANCE PROTEIN B 245 246 RXA00648 GR00169 2713 1304 MULTIDRUG RESISTANCE PROTEIN B 247 248 RXN01320 W0082 13146 11500 MULTIDRUG RESISTANCE PROTEIN B 249 250 F RXA01314 GR00382 744 4 MULTIDRUG RESISTANCE PROTEIN B 251 252 F RXA01320 GR00383 1979 1200 MULTIDRUG RESISTANCE PROTEIN B 253 254 RXN02926 W0082 11497 9866 MULTIDRUG RESISTANCE PROTEIN B 255 256 F RXA01319 GR00383 1197 4 MULTIDRUG RESISTANCE PROTEIN B 257 258 RXA01578 GR00439 1423 29 MULTIDRUG RESISTANCE PROTEIN B 259 260 RXA02087 GR00629 7076. 5730 MULTIDRUG RESISTANCE PROTEIN B 261 262 RXA02088 GR00629 8294 7080 MULTIDRUG RESISTANCE PROTEIN B 263 264 RXA00764 GR00204 3284 2169 BMRU PROTEIN Bacillus subtilis bmrU, multidrug efflux transporter 265 266 RXN03125 W0108 972 1142 Hypothetical Drug Transporter 267 268 RXN01553 W0135 25201 26520 Hypothetical Drug Permease 269 270 RXN00535 W0219 5155 5871 Hypothetical Drug Resistance Protein 271 272 RXN00453 W0076 1173 3521 Hypothetical Drug Transporter 273 274 RXN00932 W0171 13120 13593 Hypothetical Drug Transporter 275 276 RXN03022 W0002 65 511 MULTIDRUG RESISTANCE PROTEIN B 277 278 RXN03151 W0163 489 4 MYCINAMICIN-RESISTANCE PROTEIN MYRA 279 280 RXN02832 W0358 547 5 LYSOSTAPHIN IMMUNITY FACTOR 281 282 RXN00165 W0232 3275 1860 MULTIDRUG RESISTANCE-LIKE ATP-BINDING PROTEIN MDL 283 284 RXN01190 W0169 8992 10338 MULTIDRUG RESISTANCE-LIKE ATP-BINDING PROTEIN MDL 285 286 RXN01102 W0059 6128 4884 QUINOLONE RESISTANCE NORA PROTEIN 287 288 RXN00788 W0321 3424 3648 CHLORAMPHENICOL RESISTANCE PROTEIN 289 290 RXNO2119 W0102 11242 9602 A201A-RESISTANCE ATP-BINDING PROTEIN 291 292 RXN01605 W0137 7124 5610 DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN 293 294 RXN01091 W0326 567 4 MAZG PROTEIN 295 296 RXS02979 W0149 2150 2383 MERCURIC TRANSPORT PROTEIN PERIPLASMIC COMPONENT PRECURSOR 297 298 RXS02987 W0234 527 294 MERCURIC TRANSPORT PROTEIN PERIPLASMIC COMPONENT PRECURSOR 299 300 RXS03095 W0057 4056 4424 CADMIUM EFFLUX SYSTEM ACCESSORY PROTEIN HOMOLOG 2006200801 24 Feb 2006 2 Excluded Genes GenBankTMI Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A45581, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 9519442-AS5 07/20/95 A45585 A45587 AB003 132 murC; ftsQ; ftsZ Kobayashi, M. et al. "Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria," Biochem. Biophys. Res. Commun., (1997) ABC 15023 murC; ftsQ Wachi, M. et al. "A murC gene from Coryneform bacteria," Appi. Microbial.

Biolechnol., 51 (2):223-228 (1999) AB018530 dtsR Kimura, E. et al, "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium ____________lactofermentum," Biosci. Biotechnol IBiochern., 60(10):1565-1570 (1996) AB018531 dtsRl; AB020624 murl D-glutamate racemnase AB023377 tkt transketolase AB024708 gltB; gltD Glutamine 2-oxoglutarate aminotransferase large and small subunits AB025424 acn aconitase AB027714 rep Replication protein AB027715 rep; aad Replication protein; aminoglycoside adenyltransferase AF005242 argC N-acetylglutamate-5-sem ialdehyde dehydrogenase AF005635 gInA Glutamine synthetase AF030405 hisF cyclase AF030520 argG Argininosuccinate synthetase AF03 15 18 argF Ornithine carbaniolytransferase AF036932 aroD 3-dehydroquinate dehydratase AF038548 pyc Py-ruvate carboxylase 2006200801 24 Feb 2006 2 (continued) AF038651 dciAE; apt; rel Dipeptide-binding protein; adenine Wehmeier, L. et "The role of the Corynebacteriumn glutamicuin rel gene in phosphoribosyltransferase; GTP (p)ppGpp metabolism," Microbiology 144:1853-1862 (1998) pyrophosphokinase AF04 1436 argR Arginine repressor AF045998 impA Inositol monophosphate phosphatase AF048764 argH Argininosuccinate lyase AF049897 argC; argi; argB; N-acetylIglutamyl phosphate reductase; argD; argF; argR; ornithine acetylItrans ferase; NargG; argH acetylgiutamnate kinase; acetylornithine transminase; ornithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate lyase AF050 109 inhA Enoyl-acyl carrier protein reductase AF050 166 hisG ATP phosphoribosyltransferase 1846 hisA Phosphoribosylform imino-5-amino- I1phosphoribosyl-4-imidazolecarboxam ide isomerase AF052652 metA Homoserine 0-acetyltransferase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Gorynebacteriumn glutamicum," Mo!.

Cells., 8(3):286-294 (1998) AF053071 aroB Dehydroquinate synthetase AF060558 hisH Glutamine am idotransferase AF086704 hisE Phosphoribosyl-ATPpyrophosphohydrolase A F 114233 aroA 5-enolpyruvylshikimate 3-phosphate ~~synthase All 16184 panD L-aspartate-alpha-decarboxylase precursor Dusch, N. et al. "Expression of the Corynebacteriumn glutamicumn panD gene encoding L-aspartate-alpha-decarboxy lase leads to pantothenate overproduction in Escherichia coli," App. Environ. Microbiol., 65(4)1530- (1999) AF124518 aroD; aroE 3-dehydroquinase; shikimate AF124600 aroC; aroK; aroB; Chorismate synthase; shikimate kinase; 3pepQ dehydroquinate synthase; putative cytoplasmic peptidase AF145897 inhA AF145898 inhA 2006200801 24 Feb 2006 Table 2 (continued) AJO01436 ectP Transport of ectoine, glycine betaine, Peter, et al. "Corynebacterium glutamicum is equipped with four secondary proline carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine betaine carrier, EctP," J. Bacteriol., 1 80(22):6005-6012 (1998) AJ004934 dapD Tetrahydrodipicolinate succinylase Wehrmann, A. et al. "Different modes of diaminopimelate synthesis and their (incomplete') role in cell wall integrity: A study with Corynebacterium gluiamicum," J.

Bacteriot, 180(1 2):3 159-3 165 (1998) AJ1J07732 ppc; secG; amt; ocdl; Phosphoenolpyruvate-carboxy lase; high soxA affinity ammonium uptake protein; putative ornithine-cyclodecarboxylase; sarcosine oxidase AJO 103 19 ftsY, gln13, g~nD; srp; Involved in cell division; PH1 protein; Jakoby, M. et al. "Nitrogen regulation in Corynebacterium glutamicum; amtP uridylyltransferase (uridylyl-removing Isolation of genes involved in biochemical characterization of corresponding enzmye); signal recognition particle; low proteins," FEMS Microbial, 173(2):303-310 (1999) ammonium uptake protein AJ132968 cat Chloramphenicol aceteyl transferase AJ224946 mqo L-malate: quinone oxidoreductase Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dlehydrogenase (acceptor) from Corynebacterium Eur. J. Bioc/iem., 254(2):395-403 (1998) AJ238250 ndh NADH dehydrogenase AJ238703 porA Porin Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: The channel is fanned by a low mass polypeptide," Biochemistry, 37(43): 15024-15032 (1998) D17429 Transposable element IS3 1831 Vertes et al."lsolation and characterization of IS3183 1, a transposable element Corynebacterium glutamicum," Mo!. Microbiol, 11l(4):739-746 (1994) D84102 odhA 2-oxoglutarate dehydrogenase Usudla, Y. et al. "Molecular cloning of the Corynebacterium glutamicum (Brevibacterium lactofermentum AJ 12036) odhA gene encoding a novel type 2-oxoglutarate dehydrogenase," Microbiology, 142:3347-3354 (1996) E01358 hdh; hk Homoserine dehydrogenase; homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP kinase 1987232392-A 1 10/12/87 E01359 Upstream of the start codon of homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP gene 1987232392-A 2 10/12/87 E01375 Tryptophan operon E01376 trpL; trpE Leader peptide; anthranilate synthase Matsui, K. et al. "Tryptophan operon, peptidle and protein coded thereby, utilization of tryptophan operon gene expression and production of trptophan," Patent: JP 1987244382-A 1 10/24/87 2006200801 24 Feb 2006 Table 2 (continued) E01 377 Promoter and operator regions of Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, tryptophan operon utilization of tryptophan operon gene expression and production of tryptophan," Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Hatakeyama, K. et al. "DNA fragment containing gene capable of coding biotin synthetase and its utilization," Patent: JP 1992278088-A I 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04041 Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiohiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04307 Flavumn aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: JP 1993030977-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 2 03/30/93 108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1 07/27/93 E051 12 Dihydro-dipichorinate synthetase Hatakeyama, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase and its use," Patent: JP 1993 1 8437 1-A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase its use," Patent: IP 1993284970-A 1 11/02/93 E05779 Threonine synthase Kohama, K. et al. "Gene DNA coding threonine synthase and its use," Patent: 1993284972-A 1 11/02/93 E061 10 Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," JP 1993344881-A 1 12/27/93 E061 11 Mutated Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881 -A 1 12/27/93 E06 146 Acetohydroxy acid synthetase lnui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its Patent: JP 1993344893-A 1 12/27/93 E06825 Aspartokinase Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 E06826 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 2006200801 24 Feb 2006 Table 2 (continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A I 03/08/94 E07701 secY Honno, N. et al. "Gene DNA participating in integration of membraneous protein to membrane," Patent: JP 1994169780-A 1 06/21/94 E08177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08178, Feedback inhibition-released Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08180, E08181, E08182 E08232 Acetohydroxy-acid isomeroreductase Inui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," Patent: JP 1994277067-A 1 10/04/94 E08234 secE Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," Patent: JP 1994277073-A 1 10/04/94 E08643 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in synthetase promoter region coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08649 Aspartase Kohama, K. et al "DNA fragment having promoter function in coryneform bacterium," Patent: JP 1995031478-A 1 02/03/95 E08900 Dihydrodipicolinate reductase Madori, M. et al. "DNA fragment containing gene coding Dihydrodipicolinate acid reductase and utilization thereof," Patent: JP 1995075578-A 1 03/20/95 E08901 Diaminopimelic acid decarboxylase Madori, M. et al. "DNA fragment containing gene coding Diaminopimelic acid decarboxylase and utilization thereof," Patent: JP 1995075579-A 1 03/20/95 El2594 Serine hydroxymethyltransferase Hatakeyama, K. et al. "Production of L-trypophan," Patent: JP 1997028391-A 1 02/04/97 E12760, transposase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: E12759, JP 1997070291-A 03/18/97 E12758 E12764 Arginyl-tRNA synthetase; diaminopimelic Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: acid decarboxylase JP 1997070291-A 03/18/97 E12767 Dihydrodipicolinic acid synthetase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 El2770 aspartokinase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 E12773 Dihydrodipicolinic acid reductase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291-A 03/18/97 2006200801 24 Feb 2006 Table 2 (continued) E13655 Glucose-6-phosphate dehydrogenase Hatakeyama, K. et at. "Glucose-6-phosphate dehydrogenase and DNA capable of coding the same," Patent: JP 1997224661]-A 1 09/02/97 L01508 INvA Threonine dehydratase Moeckel, B. et at. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutam icum," J BacterioL, 174:8065-8072 L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et at. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3 -deoxy- D-arab inoheptu loson ate- 7-phosph ate synthase gene," FEMS MicrobioL Left., 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et at. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit; molecular analysis of the ilvB-ilvN-ilvC operon," .1 BacierioL, 175(17):559Sacid isomeroreductase 5603 (1993) L 18874 PtsM Phosphoenolpyruvate sugar Fouet, A et at. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransfe rase system: expression in Escherichia coli and homology to enzymes 11 from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, J.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme I1 and analyses of the deduced protein FEMS MicrobioL Leti., 1 19(1-2):137-145 (1994) L27 123 aceB Malate synthase Lee, H-S. et at. "Molecular c-haracterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J MicrobioL. Biotechnol, 4(4):256-263 (1994) L27 126 Pyruvate kinase Jetten, M. S. et at. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," Appi. Environ. MicrobioL, 60(7):250 1-2507 (1994) L28760 aceA Isocitrate L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et at. "Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium J Bacleriol., 1 77(2)-.465-467 (1995) M13774 Prephenate dehydratase Follettie, M.T. et at. "Molecular cloning and nucleotidle sequence of the Corynebacterium glutamicum pheA gene," J. BacterioL., 167:695-702 (1986) *M16175 5S rRNA Park, Y-H. et at. "Phylogenetic analysis of the coryneform bacteria by 56 rRNA sequences," J. BaclerioL., 169:1801-1806 (1987) *M16663 trpE Anthranilate synthase, 5' end Sano, K. et at. "Structure and function of the trp operon control regions of Brevibacterium tactofermentum, a glutam ic-acid-producing bacterium," Gene, 52:191-200 (1987) *M16664 trpA Tryptophan synthase, 3'end Sano, K. et at. "Structure and function of the tip operon control regions of Brevibacterium lactofermentum, a glutam ic-acid-producing bacterium," Gene, 52:191-200 (1987) 2006200801 24 Feb 2006 Table 2 (continued) M25819 Phosphoenolpyruvate carboxyiase O'Regan, M. et al. "Cloning and nucleotidle sequence of the Phosphoenolpyruvate carboxylase-coding gene of Corynebacterium ATCC 13032," Gene, 77(2):237-251 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J. Gen.

138:1167-1175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J. Gen.

icrobil, 138:1167-1175 (1992) M89931 aecD; brnQ; yhbw Beta C-S lyase; branched-chain amino acid Rossol, 1. et al. "The Corynebacterium glutamicum aecD gene encodes a C-S uptake carrier; hypothetical protein yhbw lyase with alpha, beta-elimination activity (hat degrades aminoethylcysteine," J. Bacteriol, 174(9):2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacteriumn glutamicum ATCC 13032 is directed by the bmnQ gene product," Arch. MicrobioL, 169(4):303-3 12 (1998) S59299 trp Leader gene (promoter) Herry, D.M. et al. "Cloning of the trp gene cluster from a tryptophanhyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence," Appl. Environ. Microbial., 59(3):791-799 (1993) UI 11545 trpD Anthranilate phosphoribosyltransferase O'Gara, J.P. and Dunican, L-K. (1994) Complete nucleotide sequence of the Corynebacteriurn glutamicum ATCC 21850 tpD gene." Thesis, Microbiology Department, University College Galway, Ireland.

U13922 cgllM; cg]lR; cIglR Putative type If 5-cytosoine Schafer, A. et al. "Cloning and characterization of a DNA region encoding a methyltransferase; putative type 11 stress-sensitive restriction system from Corynebacterium glutamnicumn ATCC restriction endonuclease; putative type I or 13032 and analysis of its role in intergeneric conjugation with Escherichia type III restriction endonuclease coli," J. Bacteriol., 176(23):7309-7319 (1994); Schafer, A. et al. "The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine in an McrBCdeficient Escherichia coli strain," Gene, 203(2):95-101 (1997) U14965 U31224 ppx Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J Bacteriol., (1996) U3 1225 proC L-proline: NADP+ 5-oxidoreductase Ankri, S. et al. "Mutations in the Corynebacterium glutamnicumproline biosynthetic pathway: A natural bypass of the proA step," J. Bacterial., 178(15):4412-4419 (1996) U31230 obg; proB; unkdh ?;gamnma glutamyl kinase;similar to D- Ankri, S. et al. "Mutations in the Corynebacterium glutamnicumproline isomer specific 2-hydroxyacid biosynthetic pathway: A natural bypass of the proA step," J Bacierial., dehydrogenases 178(1 5):44 12-4419 (1996) 2006200801 24 Feb 2006 Table 2 (continued) U31281 bioB Biotin synthase Serebriiskii, "Two new members of the bio B super-family: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacteriumn glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC Thiosulfate sulfurtransferase; acyl CoA Jager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins," Microbio., 166(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Eseherichia coi," J1 Bacterio., 179(7):2449-2451 (1997) U43536 clpB Heat shock ATP-binding U53587 aphA-3 3'S "-aminoglycoside phosphotransferase U89648 Corynebacteriumn glutam icum unidentified sequence involved in histidine biosynthesis, ~~partial X04960 trpA; trpB; trpC; trpD; Tryptophan operon Matsui, K. et al- "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium lactofermentumn tryptophan operon," Nucleic Acids Res., 13-101 14 (1986) X07563 lys A DAP decarboxylase (meso-diaminopimelate Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium decarboxylase, EC 4.1.1.20) glutamicumn and possible mechanisms for modulation of its expression," Mo!.

Genet., 212(l):1 12-119 (1988) X14234 EC 4.1.1.31 Phosphoenolpyruvate carboxylase Eikmanns, B.i. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression," Mat. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function molecular evolution," Plant. Mot. Bil., 21 (3):487-502 (1993) Xl17313 fda Fructose-bisphosphate aldolase Von der Osten, C.H. et al. "Molecular cloning, nucleotide sequence and finestructural analysis of the Corynebacterium glutamicumn fda gene: structural comparison of C. glutamicum fructose-I1, 6-biphosphate aldolase to class I and class 11 aldolases," Mo. Microb jot., X53993 dapA L-2, 3-dihydrodipicolinate synthetase (EC Bonnassie, S. et al. "Nucleic sequence of the dapA gene from Corynebacteriumn glutamicum," Nucteic Acids Res., 18(21 ):6421I (1990) X54223 AttB-related site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutam icum and the attP? site of lambdacorynephage," FEMS. Microbio, Lett., 66:299-302 (1990) X54740 argS; lysA Arginyl-tRNA synthetase; Diaminopimelate Marcel, T. et al. "Nucleotide sequence and organization of the upstream region decarboxylase of the Corynebacterium glutamicum lysA gene," Mat. Microbiol., 4(1 1):18 19- (1990) 2006200801 24 Feb 2006 Table 2 (continued) X55994 trpL; trpE Putative leader peptide; anthranilate Heery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamicum synthase component I trpE gene," Nucleic Acids Res., 18(23):7138 (1990) X56037 thrC Threonine synthase Han, K.S. et al. "The molecular structure of the Corynebacterium glutamicum threonine synthase gene," Mo!. Microbiol., 4(10):1693-1702 (1990) X56075 attB-related site Attachment site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of_ Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum ,and the attP site of lambdacorynephage," FEMS. Microbiol, Leff., 66:299-302 (1990) X57226 IysC-alpha; lysC-beta; Aspartokinase-aipha subunit; Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase asd Aspartokinase-beta subunit; aspartate beta from Corynebacterium glutamicum," Mo!. Mlicrobiol., 5(5):1 197-1204 (1991); semnialdehyde dehydrogenase Kalinowski, J1. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta-semialdehyde dehydrogenase gene asd in glutamicum," Mo. Gen. Gene., 224(3):3 17-324 (1990) X59403 gap;pgk; tpi G lycera ldehyde-3 -phosphate; Eikmanns, B.J. "Identification, sequence analysis, and expression of a phosphoglycerate kinase; triosephosphate Corynebacterium glutamicum gene cluster encoding the three glycolytic isomerase enzymes glyceraldehyde-3 -phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J. Bacieriol., 174(1 9):6076-6086 X59404 gdh Glutamate dehydrogenase Bormann, E.R. et al. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase," Mo!. Microbiol., 6(3):3 17-326 X60312 lysI L-lysine permease Seep-Feldhaus, A.H. et al. "Molecular analysis of the Corynebacterium glutamicum lysi gene involved in lysine uptake," Mo!. Microbio., 5(12):2995- (1991) X66078 cop I Ps I protein iolif'f, G. et al. "Cloning and nucleotidle sequence of the csplI gene encoding PSI, one of the two major secreted proteins of Corynebacterium glutamicum: The deduced N-terminal region of PSI is similar to the Mycobacterium antigen complex," Mol. Microbiol., 6(16):2349-2362 (1992) X661 12 glt Citrate synthase Eikmanns, B.J. et al. "Cloning sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gItA gene encoding citrate Microbiol., 140:1817-1828 (1994) X67737 dapB Dihydrodipicolinate reductase X69 103 csp2 Surface layer protein PS2 Peyret, J.L. et al. "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacterium glutamicum," Mo. Microb io!, (1993) X69104 IS3 related insertion element Bonamy, C. et al. "Identification of IS] 206, a Corynebacterium glutamicum 153-related insertion sequence and phylogenetic analysis," Mot. Microbiol., 14(3):571-581 (1994) 2006200801 24 Feb 2006 Table 2 (continued) X70959 leuA Isopropylmalate synthase Patek, M. et al. "Leucine synthesis in Corynebacteriumn glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine synthesis," Appi. Environ. Microbial, 60(l): 133-140 (1994) X71489 lcd Isocitrate dehydrogenase (NADP+) Eikmanns, BiJ. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacteriumn glutamicumn lcd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J. Bacterial, (1995) X72855 GDHA Glutamate dehydrogenase (NADP+) X75083, mtrA 5-methyltryptophan resistance Heery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of X70584 Corynebacterium glutamicum encoding resistance to Biachem. Biaphys. Res. Comimun., 201(3):1255-1262 (1994) X75085 recA Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutam icum and Brevibacterium lactofermnentum," Appi.

BiotechnaL, 42(4):575-580 (1994) X75504 aceA; thiX Partial Isocitrate lyase; Re inscheid, D.J. et al. "Characterization of the isocitrate lyase gene fr-om Corynebacterium gtutamicum and biochemical analysis of the enzyme," 176(1 2):3474-3483 (1994) X76875 ATPase beta-subunit Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Antanie Van Leeuwenhoek 64:285-305 (1993) X77034 tuf Elongation factor Tu Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Anionie Van Leeuwenhaek, 64:285-305 (1993) X77384 recA Billman-Jacobe, H. "Nucleotide sequence of a recA gene from glutamicum," DNA Seq., 4(6):403-404 (1994) X78491 aceB Malate synthase Reinscheid, D.J. et al. "Malate synthase from Corynebacteriumn glutamicum pta-ack operon encoding phosphotransacetylase: sequence analysis," 140:3099-3 108 (1994) X80629 16S rDNA 16S ribosomal RNA Rainey, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia from within the radiation of Rhodococcus species," Microbial, 141:523-528 X81 191 gluA; gluB; gluC; Glutamate uptake system Kronemeyer, W. et al. "Structure of the gluABCD cluster encoding the glu D glutamate uptake system of Corynebacteriumn glutamicum," J. Bacterial., 177(5):1152-1 158 (1995) X8 1379 dapE Succinyldiaminopimelate desuccinylase Wehrmnann, A. et al. "Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of Escherichia coli," 40:3349-56 (1994) 2006200801 24 Feb 2006 Table 21c-ontinued) X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et al "Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences," In. J Sys. Bacteriol., (1995) X82928 asd; lysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J 177(24):7255-7260 (1995) X82929 proA Gamma-glutamyl phosphate reductase Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J Bacteriol., 1 77(24):7255-7260 (1995) X84257 16S rDNA 16S ribosomal RNA Pascual, C. et al. "Phylogenetic analysis of the genus Corynebacterium based 16S rRNA gene sequences," In. J Sysi. Bacteriol, 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et al. "Functional analysis of sequences adjacent to dapE of C.

glutamicum proline reveals the presence of aroP, which encodes the aromatic amino acid transporter," J Bacterial., 1 77(20):599 1-5993 (1995) X86 157 argB; argC; argD; Acetylglutamnate kinase; N-acetyl-gamma- Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine argF; argi glutamyl-phosphate reductase; biosynthesis in Corynebacteriumn glutamicum: enzyme evolution in the early acetylomnithine aminotransferase; omithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbamoyltransferase; glutamate N- X89084 pta; ackA Phosphate acetyltransferase; acetate kinase Reinscheid, D.J. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding and acetate kinase," Microbiology, 145:503-5 13 (1999) X89850 attB Attachment site Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J Bacterial., 1996-2004 (1996) X90356 Promoter fragment Fl Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90357 Promoter fragment F2 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90358 Promoter fragment FlO Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90359 Promoter fragment F13 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) 2006200801 24 Feb 2006 2 (continued) X90360 Promoter fragment F22 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90361 Promoter fragment F34 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90362 Promoter fragment F37 Patek, M. et al. "Promoters from C. glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90363 Promoter fragment F45 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a .consensus motif," Microbiology, (1996) X90364 Promoter fragment F64 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90365 Promoter fragment F75 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90366 Promoter fragment PF 10 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology (1996) X90367 Promoter fragment PF104 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90368 Promoter fragment PFI109 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology (1996) X93513 amt Ammonium transport system Siewe, R.M. et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier of Corynebacterium glutamicum," J Biol. Chemn., 27]10):5398-5403 (1996) X93514 betP Glycine betaine transport system Peter, H. et al. "Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the solute glycine betaine," J Bacteriol., 178(17):5229-5234 (1996) X95649 orf4 Patek, M. el al. "Identification and transcriptional analysis of the dapB-ORF2dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis," Biotechnol. Left., 19:1113-1117 (1997) X96471 lysE; lysG Lysine exporter protein; Lysine export Vrljic, M. et al. "A new type of transporter with a new type of cellular regulator protein function: L-lysine export from Corynebacterium glutamicum," MoL Microbiol., 22(5):815-826 (1996) 2006200801 24 Feb 2006 Table 2 (continued) X96580 panB; panC; xyIB 3-methyl-2-oxobutanoate Sahm, et al. "D-pantothenate synthesis in Corynebacterium glutamicum and hydroxymethyltransferase; pantoate-beta- use of panBC and genes encoding L-valine synthesis for D-pantothenate alanine ligase; xylulokinase overproduction," App!. Environ. Microbiol., 65(5):1973-1979 (1999) X96962 Insertion sequence IS 1207 and transposase X99289 Elongation factor P Ramos, A. et al. "Cloning, sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermnentum ___________(Corynebacterium glutamicum ATCC 13869)," Gene, 198:217-222 (1997) Y00140 thrB Homoserine kinase Mateos, L.M. et al. "Nucleotide sequence of the homoserine kinase (thrB) gene of the Brevibacterium lactofermentum," Nucleic Acids Res., 15(9):3 922 (1987) Y00151 ddh Meso-diaminopimelate D-dehydrogenase Ishino, S. et al. "Nucleotide sequence of the meso-d iamninopim elate D- (EC 1.4.1.16) dehydrogenase gene from Corynebacteriurn glutamicum," Nucleic Acids Res., (1987) Y00476 thrA H-omoserine dehydrogenase Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacteriumn lactofermentum," Nucleic Acids Res., (1987) Y00546 horn; thrB Homoserine dehydrogenase; homoserine Peoples, O.P. et al. "Nucleotide sequence and fine structural analysis of the kinase Corynebacterium glutamicum hom-thrB operon," MoI. Microbiol., 2(1):63-72 (1988) Y08964 murC; flsQ/divD; ftsZ UPD-N-acetylmuramate-alanine ligase; Honrubia, M.P. et al. "Identification, characterization, and chromosomal division initiation protein or cell division organization of the ftsZ gene from Brevibacteriumn lactofermentum," Mot. Gen.

protein; cell division protein Genei., 259(1):97-104 (1998) Y09 163 putP High affinity proline transport system Peter, H. et al. "Isolation of the putP gene of Corynebacterium glutamnicumproline and characterization of a low-affinity uptake system for compatible solutes," Arch. lvfcrobiol., 168(2): 143-151 (1997) Y09548 pyc Pyruvate carboxylase Peters- Wend isch, P.G. et al. "Pyruvate carboxylase from Corynebacteriumn glutamicum: characterization, expression and inactivation of the pyc gene," ___________Microbiology, 144:915-927 (1998) Y09578 leuB 3-isopropylmalate dehydrogenase Patek, M. et al. "Analysis of the leuB gene from Goz-ynebacterium glutamicum," App!. Microbial. Bioleclinol., 50(1l):42-47 (1998) Y 12472 Attachment site bacteriophage Phi- 16 M'voreau, S. et al. "Site-specific integration of corynephage Phi- 16: The construction of an integration vector," Microb jot., 145:539-548 (1999) Y12537 proP Proline/ectoine uptake system protein Peter, H. et al. "Corynebacterium glutamicumn is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine betaine carr ier, EctP," J. Bacieriof., 180(22):6005-6012 (1998) 2006200801 24 Feb 2006 Table 2 (continued) Y 13221 gInA Glutamine synthetase I Jakoby, M. et al. "Isolation of Corynebacterium glutamicumn glnA gene glutamine synthetase FEMS Microbial Let., 154(1l):8 1-88 (1997) Y 16642 lpd Dihydrol ipoamide dehydrogenase Y 18059 Attachment site Corynephage 304L Moreau, S. et al. "Analysis of the integration functions of &phi;304L: An module among corynephages," Virology, 255(1):150-159 (1999) Z21501 argS; lysA Arginyl-tRNA synthetase; diarninopimelate Oguiza, i.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the decarboxylase (partial) upstream region of the lysA gene in Brevibacterium lactofermentum: Regulation of argS-lysA cluster expression by arginine," J.

Bacterial., 175(22):7356-7362 (1993) Z21502 dapA; dapB Dihydrodipicolinate synthase; Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, and dapB) of dihydrodipicolinate reductase Brevibacterium lactofermentum encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function," J. Bacterial., 175(9):2743-2749 (1993) Z29563 thrC Threonine synthase Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded threonine synthase," AppI. Environ. Mlicrobial., 60(7)2209-22 19 (1994) Z46753 16S rDNA Gene for 16S ribosomal RNA Z49822 sigA SigA sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacteriumn lactofermentum: Characterization of sigA and sigB," J. Bacterial., 178(2):550- 553 (1996) Z49823 galE; dtxR Catalytic activity UDP-galactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of epimerase; diphtheria toxin regulatory Brevibacterium lactofermentumn is coupled transcriptionally to the dmdR gene," Gene, 177:103-107 (1996) Z49824 orfi1; sigB SigB sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J Baclerial, 178(2):550- 553 (1996) Z66534 Transposase Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacteriumn lactoferrnentumn ATCC 13869," Gene, 'TA sequence for this gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

74 TABLE 3: Corynebacterium and Brevibacterium Strains W~hich May be Used in the Practice of the Invention Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 19351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes 19353 Brevibacterium ammoriagenes 19354 Brevibacterium ammoniagenes 19355 Brevibacterium ammoniagenes 19356 Brevibacterium ammoniagenes 21055 Brevibacterium ammoniagenes 21077 Brevibacterium ammoniagenes 21553 Brevibacterium ammoniagenes 21580 Brevibacterium ammoniagenes 39101 Brevibacterium butanicum 21196 Brevibacterium divaricatum 21792 P928 Brevibacterium flavum 21474 Brevibacterium flavum 21129 Brevibacterium fiavum 21518 Brevibacterium flavum B 1474 Brevibacterium flavum BI 11472 Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum Brevibacterium flavum 21517 Brevibacterium flavum 21528 Brevibacterium flavum 21529 Brevibacterium flavum B1 14771___ Brevibacterium flavum BI 1478 Brevibacterium flavum 21127 Brevibacterium flavum BI 1474 Brevibacterium healii 15527 Brevibacterium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacterium lactoferrnentum Bf re-vibacterium lactofenmentum 74 Brevibacterium lactofermentum 77 Brevibacterium lactofermentum 21798 Brevibacterium lactofermentum 21799 Brevibacterium lactofermentum 21800 Brevibacterium lactofermentum 21801 Brevibacterium lactofermentum Bi 11470 Brevibacterium lactofermentum BI 1471 75 Brevibacterium lactofermentumn 21086 Brevibacterium lactofermentumn 21420 Brevibacterium lactofermenturm 21086 Brevibacterium lactofermentumn 31269 Brevibacterium linens 9174 Drevibacterium linens 19391 Brevibacterium linens 8377 Brevibacterium paraffinolyticumn 11160 Brevibacterium Spec. ____717.73 Brevibacterium Spec. ____717.73 Brevibacterium Spec. 14604 Brevibacteriumn Spec. 21860 Brevibacterium Spec. 21864 Brevibacteriumn Spec. 21865 Brevibacterium Spec. 21866 Brevibacterium Spec. 19240 Corynebacterium acetoacidophilum 21476 Corynebacterium acetoacidophilumn 13870 Corynebacterium acetoglutamnicumn B1 11473 Corynebacterium acetoglutamnicumn B1 11475 Corynebacterium acetoglutamnicumn 15806 Corynebacterium acetoglutam icumn 21491 Corynebacterium acetoglutamnicumn 31270 Corynebacterium acetophilum B3671 CorynebacteriuM ammoniagenes 6872 2399 Corynebacterium ammoniagenes 15511 Corynebacterium fujiokense 21496 Corynebacterium glutamnicumn 14067 Corynebacterium glutamnicumn 39137 Corynebacterium glutamnicumn 21254 Corynebacterium glutamnicumn 21255 Corynebacterium glutamicum 31830 Corynebacterium glutamnicumn 13032 Corynebacterium glutamnicumn 14305 Corynebacterium glutamicumn 15455 Corynebacterium glutamnicumn 13058 Corynebacterium glutamnicumn 13059 Corynebacterium glutamicum 13060 Corynebacterium glutamnicumn 21492 Corynebacterium glutamicum 2 15 13 Corynebacterium glutamnicumn 21526 Corynebacterium glutamnicumn 21543 Corynebacterium glutamnicumn 13287 Corynebacterium glutamicum 21851 Cor'ynebacterium glutamnicumn 21253 Corynebacterium glutamnicumn 21514 Corynebacterium glutamicum 21516 Corynebacterium glutamnicumn 21299 76 iW We eciE M N1~C~ NLB~ S A S~ Corynebacterium glutamicum 21300 Corynebacterium glutamicurn 39684 Corynebacterium glutarnicum 21488 Corynebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutamicum 21158 Corynebacteriuni glutamicum 21159 Corynebacterium glutamicum 21355 Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutam icum 21565 Corynebacterium glutamicum 21566 Corynebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacteriurr glutamicum 21572 Corynebacterium glutamicum 21573 Corynebacterium glutarnicum 21579 Corynebacterium glutaxnicum 19049 Corynebacterium glutamicum 19050 Corynebacterium glutamicum 19051 Corynebacterium glutamicum 19052___ Corynebacterium glutamicum 19053 Corynebacterium glutamicum 19054___ Corynebacterium glutamicum 19055 Corynebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium glutainicum 19058 Corynebacterium glutamicum 19059 Corynebacterium glutamicuni 19060 Corynebacterium glutamicum 19185 Corynebacterium glutamicum 13286 Corynebacterium glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium glutamicum 21544 Corynebacterium glutamicum 21492 Corynebacterium glutamicum B8183 Corynebacterium glutamicum B8 182 Corynebacterium glutamicum B 12416 Corynebacterium glutamicum B12417 77 C57 N R" Er jJM T" S Corynebacterium glutaniicurn Bl12418 Corynebacterium glutamicum B 11476 Corynebacterium glutamnicum 21608 Corynebacterium lilium P973____ Corynebacterium nitrilophilus 21419 11594 Corynebacteriumn spec. _____P4445 Corynebacterium spec. P4446 Corynebacteriumn spec. 31088 Corynebacterium spec. 31089 Corynebacterium spec. 31090 Corynebacteriuni spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 ___20145 Corynebacterium spec. 21857 Corynebacterium spec. 21862 ,Corynebacterium ,spec. 21863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermnentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, 1L, USA CECT Coleccion Espanola de Cultivos Tipo, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Ccntraalbureau voor Schimmelcultures, Baam, NL NCTC: National Collection of Type Cultures, London, UK DSMZ: Deutsche Sammiung von Mikroorganismen und Zelikulturen, Braunschweig, Germany For reference see Sugawara, H. et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fungi and yeasts (4h~ edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

2006200801 24 Feb 2006 TABLE 4: ALIGNMENT RESULTS Lenath Accession Name of Genbank Hit ID length Genbank Hit

(NT)

Source of Genbank Hit homology Date of (GAP) Deposit 39,080 5-Jun-99 rxaOOO62 1521 GBHTG2:AC007366 185001 AC007366 Homo sapiens clone NH0501G22, SEQUENCING IN PROGRESS 3 unordered pieces.

H-omo sapiens Homo sapiens Brassica nigra Homo sapiens Homo sapiens rxaOOO84 948 GBPR3:HSU80741 GBPL1:BNDNATRNA GBPR3:HSU80741 rxaOOlO9 735 GBGSS9:AQ163721 GBHTG4:AC007054 GBHTG4:AC007054 rxa00215 1449 GBBA1:5C9C7 GBBA1:SCE94 GBBA2:AF1 10185 912 U80741 Homo sapiens CAGH44 mRNA, partial cds.

1732 X89901 B.nigra DNA for tRNA like gene.

912 U80741 Homo sapiens CAGH44 mRNA, partial cds.

388 AQ163721 HS_-2245_AlF07_MF CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2245 Col=13 Row=K, genomic survey sequence.

171979 AC007054 Drosophila melanogaster chromosome 2 clone BACR45018 (D527) RPCI-98 45.0.18 map 41E-41E strain y: cn bw sp, SEQUENCING IN PROGRESS-, 13 unordered pieces.

171979 AC007054 Drosophila melanogaster chromosome 2 clone BACR4501 8 (D527) RPCI-98 45.0.18 map 41 E-41 E strain y; cn bw sp. SEQUENCING IN PROGRESS-, 13 unordered pieces.

31360 AL035161 Streptomyces coelicolor cosmid 9C7.

38532 AL049628 Streptomyces coelicolor cosmid E94.

20302 AF110185 Burkholderia pseudomallei strain 1026b DbhB (dbhB), general secretory pathway protein D (gspD), general secretory pathway protein E (gspE), general secretory pathway protein IF (gspF), GspC (gspC), general secretory pathway protein G (gspG). general secretory pathway protein H (gspH). general secretory pathway protein I (gspl), general secretory pathway protein J (gspJ). general secretory pathway protein K (gspK). general secretory pathway protein L (gspL), general secretory pathway protein M (gspM), and general secretory pathway protein N* (gspN) genes, complete cds; and unknown genes.

384 N80167 za65g02.sl Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE:297458 3T, mRNA sequence.

384 G37084 SHGC-56832 Human Homo sapiens STS genomic, sequence tagged site.

384 G37084 SHGC-56832 Human Homo sapiens STS genomic, sequence tagged site.

Drosophila melanogaster 36,589 Drosophila melanogaster 36,589 Streptomyces coelicolor Streptomyces coelicolor Burkholderia pseudomallei Homo sapiens Homo sapiens 39,264 36.725 38,957 45,066 18-DEC-i 997 6-Feb-97 18-DEC-1997 16-OCT-i1998 13-OCT-i 999 1 3-OCT-i1999 44.444 36,313 44,159 1 2-Jan-99 12-Apr-99 2-Aug-99 rxa00289 1299 GBEST6:N80167 GBSTS:G37084 40,420 29-MAR-1996 40,420 30-MAR-1998 40,420 30-MAR-1998 GBSTS:G37D84 Homo sapiens rxa00404 2439 GBBA1:MTCY22D7 31859 Z83866 Mycobacterium tuberculosis H37Rv complete genome: segment 133/162.

GBBA1:ECU82598 GB BA2:AEDOO1 65 rxa00479 2313 GBBA1:SCF43A 136742 U82598 12003 AE000165 35437 AL096837 Escherichia coli genomic sequence of minutes 9 to 12.

Escherichia coli K-i 2 MG1 655 section 55 of 400 of the complete genome.

Streptomyces coelicolor cosmid F43A.

Mycobacterium tuberculosis Escherichia coli Escherichia coi Streptomyces coelicolor A3(2) 60,271 54,256 54,256 36,245 17-Jun-98 1 5-Jan-97 12-Nov-98 13-Jul-99 26-Jul-99 GB-GSS2:CNS015U4 1036 AL105910 Drosophila melanogaster genomne survey sequence SP6 end of SAC 1BACN14GO8 Drosophila melanogaster 37.573 of DrosBAC library from Drosophila melanogaster (fruit fly). genomic survey sequence.

2006200801 24 Feb 2006 GBPR3:H5A494O16 rxa00497 420 GBBA1:MTCY78 GBBA2:AF079544

GB_BAI:MTGROEOP

50502 33818 817 2987 AL1 17328 Z77 165 AF079544 X60350 Table 4 (continued) Human DNA sequence from clone 494016 on chromosome 22, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 145/162.

Mycobacterium aviumn GroESL operon. partial sequence.

M~tuberculosis groE gene for KCS and 1 0-kDa products.

Homo sapiens Mycobacterium tuberculosis Mycobacteriumn aviumn Mycobacteriumn tuberculosis 36,475 40,250 64,439 62,857 23-Nov-99 17-Jun-98 16-Aug-98 23-Apr-92 rxa00575 rxa00599 510 GBGSS1O:AQ199703 439 AQ 199703 GB-PR2:AC002127 144165 AC002127 GB-STS:G51234 439 G51234 rxaOO600 1221 GBBAi:MTCY441 35187 Z80225 GBBAI:MSGY223 42061 AD000019 GBBA1:BSUBO0i4 213420 Z99117 nca00605 1603 GBBA2:AF069070 2776 AF069070 GB_BA1:OVCAT 1845 X82176 GBBA1:SC2G5 38404 AL035478 rxa00648 1533 GBHTG1:HS74016 169401 AL110119 GBHTGI:HS74016 169401 AL1119 GB-HTG1:HS74016 169401 AL110119 rxa00764 1239 GBEST36:A1898007 609 Al1898007 GBBA2:PAU93274 8008 U93274 GBBA2:PAU93274 8008 U93274 rxa0O803 1353 GB_1N2:CELH34CO3 27748 AF100662 GBHTG2:AC007905 100722 AC007905 GBHTG2:AC007905 100722 AC007905 RPCII 1-460i3.TJ RPCI-i 1 Homo sapiens genomic clone RPCI-1 1-46013, genomic survey sequence.

Human BAC clone RG305H12 from 7q21. complete sequence.

SHGC-80708 Human Homo sapiens STS genomic, sequence tagged site.

Mycobacterium tuberculosis H37Rv complete genome, segment 118/162.

Mycobacterium tuberculosis sequence from clone y223.

Bacillus subtilis complete genome (section 14 of 21): from 2599451 to 2812870.

Endosymbiont of Onchocerca volvulus catalase gene, complete cds.

Onchocerca volvulus endobacterial mRNA for catalase.

Streptomyces coelicolor cosmid 2G5.

Homo sapiens chromosome 21 clone RPCIP70401 674 map 21q21, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 21 dlone RPCIP70401674 map 2 1q21, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 21 clone RPCIP70401 674 map 21q21, SEQUENCING IN PROGRESS in unordered pieces.

EST267450 tomato ovary, TAMU Lycopersicon esculentumn cONA clone cLED31K22, mRNA sequence.

Pseudomonas aeruginosa YafE (yafE), LeuB (leuB), Asd (asd). FimV (fimV). and HisT (hisT) genes, complete cds; TrpF (trpF) gene, partial cds; and unknown Pseudomonas aeruginosa YafE (yafE), LeuB (leuB), Asd (asd), FimV (fimV), and HisT (hisT) genes, complete cds; TrpF (trpF) gene, partial cds; and unknown Caenorhabditis elegans cosmid H34C03.

Homo sapiens chromosome 16q24.3 clone PAC 754F23, SEQUENCING IN PROGRESS 33 unordered pieces.

Homo sapiens chromosome 16q24.3 clone PAC 754F23,~ SEQUENCING IN PROGRESS unordered pieces.

Homo sapiens 42,657 Homo sapiens 37,052 Homo sapiens 42.657 Mycobacteriumn 56,183 tuberculosis Mycobacteriumn 37.217 tuberculosis Bacillus subtilis 36,553 endosymbiont of 55,396 Onchocerca volvulus endosymbiont of 55,396 Onchocerca volvulus Streptomyces coelicolor 39,530 Homo sapiens 36,327 Homo sapiens 36.327 Homo sapiens 35,119 Lycopersicon esculentum 34,323 Pseudomonas 35,895 aeruginosa Pseudomonas 41,417 aeruginosa Caenorhabditis elegans 34,152 Homo sapiens 37,472 Homo sapiens 37.472 20-Apr-99 27-MAY-i1997 25-Jun-99 18-Jun-98 10-DEC-i1996 26-Nov-97 25-Nov-98 26-Nov-98' 11 -Jun-99 27-Aug-99 27-Aug-99 27-Aug-99 27-Jul-99 23-Jun-98 23-Jun-98 28-OCT-i1998 24-Jun-99 24-Jun-99 2006200801 24 Feb 2006 rxa0O810 324 GBBA1:MTYi5C1O 33050 Z95436 GBBAi:MLCB2548 GB BA1:ECOUW76 GBBA1:SC5C7 GB-BA1 :SC5F2A rxa00829 2463 38916 AL023093 225419 U00039 41906 AL031515 40105 AL049587 3374 L76359 15916 Z77250 Table 4 (continued) Mycobacterium tuberculosis H-37Rv complete genome: segment 154/1 62.

Mycobacterium Ieprae cosmid 82548.

E. coi chromosomal region from 76.0 to 81.5 minutes.

Streptomyces coelicolor cosmid 5C7.

Streptomyces coelicolor cosmid 5F2A.

Streptomyces peucetius daunorubicin resistance protein (drrC) gene, complete Mycobacterium tuberculosis H37Rv complete genome; segment 113/162.

Mycobacterium tuberculosis Mycobacterium leprae Escherichia coli Streptomyces coelicolor Streptomyces coelicolor 34,615 52,997 65.269 37,490 27-Aug-99 7-Nov-96 7-Sep-98 24-MAY-i1999 24-DEC-i1996 17-Jun-98 34,615 17-Jun-98 GBBA1:STMDRRC rxa00843 468 GB-BA1 :MTCY9C4 Streptomyces, peucetius 55,279 Mycobacteriumn 40,000 tuberculosis Mycobacteriumn 37,773 tuberculosis GB-BAI:MTCY9C4 15916 Z77250 Mycobacterium tuberculosis H37Rv complete genome; segment 113/162. 17-Jun-98 rxa00858 568 GBBA1:SCC54 GB-EST1 8:N966i 0 GS EST1 8:T45493 rxa00886 1269 GB-BA1:SYCSLLLH GBBA1:SCDNAJ

GBBAI:STMDNAK

30753 547 436 132106 5611 4648 AL035591 N96610 T45493 D64006 X77458 L46700 ncaOO900 975 GBBA2:ECOUW67O0 110000 U1B997 GB-BA2:ECOUW67_0 110000 U18997 GB_8A2:AE000393 10516 AE000393 537 GBHTG3:AC010757 175571 AC010757 GB_-TG3:AC010757 175571 AC010757 GBHTG3:AC01 1283 87295 AC01 1283 rxa00981 753 GBOV:GGA245664 512 AJ245664 GBPL2:AC007887 159434 AC007887 GB-GSS1 :CNSOORNW542 AL087338 rxaOO995 864 GBEST29:A1553951 450 A1553951 GB-PR3:AC003029 139166 AC003029 Streptomyces, coelicolor cosmid C54.

21285 Lambda-PRL1 Arabidopsis thaliana cONA clone FIOG3T7, mRNA 8756 Lambda-PRL2 Arabidlopsis thaliana cDNA clone 133C1 4T7, mRNA Synechocystis sp. PCC6803 complete genome, 25/27, 3138604-3270709.

Scoelicolor dnaK grpE and dnaJ genes.

Streptomyces coelicolor (strain A3(2)) dnaK operon encoding molecular chaperones (dnaK. dnaJ). grpE and hspR genes, complete cds's.

Escherichia coli K-12 chromosomal region from 67.4 to076.0 minutes.

Escherichia coli K-12 chromosomal region from 67.4 to 76.0 minutes.

Escherichia coi K-12 MG1655 section 283 of 400 of the complete genome.

Homo sapiens chromosome 18 clone 128_C_18 map 18, **SEQUENCING IN PROGRESS 20 unordered pieces.

H-omo sapiens chromosome 18 clone 128C.1 8 map 18, -SEQUENCING IN PROGRESS 20 unordered pieces.

Homo sapiens clone M52016A09, **SEQUENCING IN PROGRESS 1 Streptomyces coelicolor Arabidopsis thaliana Arabidopsis thaliana Synechocystis sp.

Slreptomyces coelicolor Streptomyces coelicolor Escherichia coli Escherichia coli Escherichia coi Homo sapiens Homo sapiens Homo sapiens 39,602 37,801 34,194 37,459 49.744 49,583 38,314 37,759 38,314 34.857 11 -Jun-99 5-Jan-98 4-Aug-98 13-Feb-99 21-Nov-96 22-Nov-96 U I99 I U18997 C 12-Nov-98 22-Sep-99 unordered pieces.

Gallus gallus partial mRNA for ATP-citrate lyase (ACL gene). Gallus gallus Genomic sequence for Arabidlopsis thaliana BAC F1504 from chromosome I. Arabidlopsis thaliana complete sequence.

Arabidlopsis thaliana genome survey sequence T7 end of BAC F14D37 of IGF Arabidopsis thaliana library from strain Columbia of Arabidlopsis thaliana, genomic survey sequence.

teS4d0l.xl Soares NFLTGBC_51 1-omo sapiens cDNA clone IMAGE:2090497 H-omo sapiens 3 similar to gb:X02067 H.sapiens mRNA for 7SL RNA pseudogene (HUMAN);, mRNA sequence.

Homo sapiens Chromosome 12q24 PAC RPCI3-462E2 (Roswell Park Cancer H-omo sapiens Institute Human PAC library) complete sequence.

Erwinia amylovora srlA, srlE, srlB, srlD, srlM and srIR genes. Erwinia amnylovora Archaeoglobus fulgidus section 106 of 172 of the complete genome. Archaeoglobus fulgidus AV01 8764 Mus musculus 18-day embryo C57BUJ6J Mus musculus cONA clone Mus musculus 1 190006M16, mRNA sequence.

34,857 22-Sep-99 35,448 07-OCT-i1999 37,536 28-Sep-99 37,600 04-OCT-i1999 41,264 28-Jun-99 42,627 13-Apr-99 38,915 17-Sep-98 GB BA1:EAY14603 rxa00996 864 GBBA2:AEO01001 8764 4479 10730 242 Yi14603 AE001 001 AV01 8764 37,694 41,078 39,669 6-Jan-98 15-DEC-1997 28-Aug-99 2006200801 24 Feb 2006 GBGSS3:B24189 377 1242 GBOV:AF007068 356 GB-EST10:AA166324 514 GBEST7:W89968 46 732 GB-GSS12:AQ381423 579 GB-H~TG6:AC010901 206121 GBGSS5-:AQ746932 837 rxa01052 432 GBIN1:CELC13D9 43487 GBIN1:CELC13D9 43487 ncaOlO53 543 GB_OV:CHKMAFG1 1316 GBHTG6.AC010765 146468 GBHTG6:AC010765 146468 rxaClOS4 612 GB PL1:PHNPNGLP 962 GB-HTG2:HSJ402N21 170302 GBHTG2:HSJ4O2N21 170302 rxaOI2l7 723 GB_1N2:CELF18A12 29784 GB_lN2:CELF18AI2 29784 GB_RO:MUSMCFTR .6304 Table 4 (continued) 824189 F1I9E1I6TF IGF Arabidlopsis thaliana genomic clone F1 9E1 6, genomic survey sequence.

AF007068 Coturnix cotumix arylalkylamine N-acetyltransferase mRNA, partial cds.

AA166324 ms5OcO9.rl Life Tech mouse embryo 13 5dpc 10666014 Mus musculus cDNA clone IMAGE:614992 5' similar to SW:NEST RAT P21263 NESTIN. mRNA sequence.

W89968 mf64gll1.rl Soares mouse embryo NbMEI13.5 14.5 Mus musculus cDNA clone IMAGE:41 9108 5' similar to SW:NEST RAT P21263 NESTIN. [1J; mRNA sequence.

AQ381423 RPCIl11-1 35FI 0.TJ RPCI-i 1 Homo sapiens genomic clone RPC 1-11-1 35F 10, genomic survey sequence.

AC010901 Homo sapiens clone RPI1-544J22. WORKING DRAFT SEQUENCE, 1 unordered pieces.

AQ746932 HS_5538_AlAll1_T7A RPCI-1 1 Human Male SAC Library Homo sapiens genomic clone Platel1 114 001=21 Row=A, genomic survey sequence.

AF016420 Caenorhabditis elegans cosmid Cl 309.

AF016420 Caenorhabditis elegans cosmid C1 309.

028601 Chicken novel maf-related gene mafG encoding bZip nuclear protein MafG, promoter region and exon 1.

AC010765 Homo sapiens clone RP1 1-1 15N5. -SEQUENCING IN PROGRESS ~,26 unordered pieces.

AC01 0765 Homo sapiens clone RP1 1-1 15N6, -SEQUENCING IN PROGRESS m,26 unordered pieces.

045425 Pharbitis nil mRNA for Pharbitis nil Germ in-like protein precursor, complete ccds.

AL049553 Homo sapiens chromosome 6 clone RP3-402N21 map p2l1- 2 1.31, -SEQUENCING IN PROGRESS in unordered pieces.

AL049553 Homo sapiens chromosome 6 clone RP3-402N21 map p21.1-21.31.

-SEQUENCING IN PROGRESS in unordered pieces.

AF016688 Caenorhabditis elegans cosmid F1 8A1 2.

AF016688 Caenorhabditis elegans cosmid F1 BAi2.

M60493 Mouse cystic fibrosis transmembrane conductance regulator (CFTR) mRNA, complete cds.

AF031037 Neisseria meningitidis chloramphenicol acetyltransferase gene, complete cds.

AL109815 Plasmodium falciparum chromosome 13 strain 307, SEQUENCING IN PROGRESS in unordered pieces.

AL109815 Plasmodium falciparum chromosome 13 strain 3D7, SEQUENCING IN PROGRESS in unordered pieces.

AC005224 Homo sapiens chromosome 17. clone hRPK.214-_1, complete sequence.

AC005224 Homo sapiens chromosome 17, clone hRPK.214_0-1, complete sequence.

AC01 1500 Homo sapiens chromosome 19 clone CIT978SKB_60E1 11, SEQUENCING IN PROGRESS 246 unordered pieces.

AC010831 Homo sapiens clone 6_L-24, LOW-PASS SEQUENCE SAMPLING.

Arabidopsis thaliana Coturnix coturnix Mus musculus Mus muscutus Homo sapiens Homo sapiens Homo sapiens Caenorhabditis elegans Caenorhabditis elegans Gallus gallus Homo sapiens Homo sapiens lpomoea nil H-omo sapiens Homo sapiens Caenorhabditis elegans Caenorhabditis elegans Mus mnusculus Neisseria meningitidis Plasmodium falciparumn Plasmodium falciparum Homo sapiens Homo sapiens Homo sapiens 44.385 46,629 38,677 10-OCT-i1997 12-Jul-97 19-DEC-1996 58,696 12-Sep-96 37,651 36,011 38,640 39,344 38,780 39,205 32,961 38,476 42,925 36,825 36,825 35,794 40,625 37,793 35,014 17.697 17,697 38,195 36,6111 36.446 21-MAY-1999 04-DEC-i1999 19-Jul-99 2-Aug-97 2-Aug-97 7-Feb-99 07-DEC-i1999 07-DEC-i1999 10-Feb-99 03-DEC-i1999 03-DEC-1 999 08-OCT-1 999 08-OCT-i1999 10-Jun-94 21-Apr-98 19-Aug-99 19-Aug.99 14-Aug.98 14-Aug-98 AC01 1500 rxa0l320 1770 GB-TG1 :FM13P81478 GBHTGl:PFMALi3PA8O518 rxa01345 1575 GB-PR3:AC005224 166687 GB-PR3:AC005224 166687 GBHTG3:ACO11500_1300851 rxaOl4O7 1014 GB-HTG3:AC010831 70233 Homo sapiens 35,764 23-Sep-99 2006200801 24 Feb 2006 GBMHTG3:AC010831 GBPR3:AC004058 rxa0l4OB 324 GBPR4:AF152365 GBMTG3:AC007890 70233 AC010831 38400 AC004058 246546 AF152365 121256 AC007890 Table 4 (continued) Homo sapiens clone 6_L -24, LOW-PASS SEQUENCE SAMPLING. Homo sapiens 35,764 Homo sapiens chromosome 4 clone 824 1P19 map 4q25. complete sequence. Homo sapiens 40,778 Homo sapiens constitutive fragile region FRA38 sequence. Homo sapiens 41,234 Drosophila metanogaster chromosome 3 clone BACRO2G211 (D722) RPCI-98 Drosophila melanogaster 39,432 02.G.21 map 90E-91A strain y; con bw sp, SEQUENCING IN PROGRESS-.

89 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACR02G211 (0722) RPCI-98 Drosophila melanogaster 39,432 02.G.21 map 90E-91A strain y; cii bw sp, **SEQUENCING IN PROGRESS-, 89 unordered pieces.

Bacillus subtilis complete genome (section 15 of 21): from 2795131 to3013540. Bacillus subtilis 38,201 Drosophila melanagaster chromosome 2 clone BACR13J1O (D924) RPCI-98 Drosophila melanogaster 38,302 13.1.10 map 47B3-47C strain y; cn bw sp, SEQUENCING IN PROGRESS-, 82 rxa01524 1566 GBHTG3:AC007890 121256 AC007890 GBBA1:BSUBOO15 218410 Z99118 GBHTG2:AC008260 107439 AC008260 GBHTG2:AC008260 107439 AC008260 23-Sep-99 30-Sep-98 1-Aug-99 3-Sep-99 3-Sep-99 26-Nov-97 2-Aug-99 2-Aug-99 rxa01578 1510 nca0l6l6 1605 nxa01666 1500 rxa01674 1017 GBPR4:AF1 11170 GBPR4:AF1 11170 GBBA1:AEY13732 GBBA2:AF088857 GB IN1 :CEMO4D8 GBE5T25:A1281910 148083 AF11117O 148083 AF111170 6740 Y13732 2908 AF088857 21552 Z32682 276 A1281910 GBBAl :CGU43535 2531 U43535 GBHTG3:AC009213 114735 AC009213 GBHTG3:AC009213 114735 AC009213 GBPLI:A8017159 1859 AB017159 GB_ PR1:H1JMGN054823142 D26607 GBHTG3:AC01 1234 154754 AC01 1234 GBHTG3:AC009450 124337 AC009450 GBl-HTG3:AC009450 124337 AC009450 GB-HTG3:AC009919 134724 AC009919 GBBA1:ECONEUC 1676 M84026 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR13J1O (13924) RPCI-98 13.J.10 map 4713-47C strain y; cn bw sp, SEQUENCING IN PROGRESS 82 unordered pieces.

Homo sapiens 14q32 Jagged2 gene, complete cdls; and unknown gene.

Homo sapiens 14q32 Jagged2 gene, complete cds; and unknown gene.

Alcaligenes eutrophus genes for ureases, ureDi, ureD2, ureA, ureB, and ORF1, ORF2.

Vogesella indigotera indligoidine biosynthesis regulatory locus, complete Caenorhabditis elegans cosmid M04D8, complete sequence.

qt82d04.xl NClCGAP-Co1 4 Homo sapiens cDNA clone IMAGE: 1961767 3% mRNA sequence.

Corynebacteriumn glutamicum multidrug resistance protein (cmr) gene, complete cds.

Drosophila melanogaster chromosome 3 clone BACR09F18 (0812) RPCI-98 09TF.18 map 980-98D strain y; cn bw sp, **SEQUENCING IN PROGRESS 109 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO9F18 (0812) RPCI-98 09.F.1 8 map 980-98D strain y; cn bw sp, SEQUENCING IN PROGRESS-,~ 109 unordered pieces.

Daucus carota mRNA for citrate synthase. complete cds.

Homo sapiens endlothelial nitric oxide synthase gene, complete cds.

Hama sapiens clone NH-0166D323, SEQUENCING IN PROGRESS 7 unordered pieces.

Homo sapiens chromosome 9 clone 30 -C 23 map 9, SEQUENCING IN PROGRESS 20 unordered pieces.

Hama sapiens chromosome 9 clone 30-C_23 map SEQUENCING IN PROGRESS 20 unordered pieces.

Homo sapiens clone 1151I23, LOW-PASS SEQUENCE SAMPLING.

Ecoli protein p7 (neu C) gene, complete cds.

Drosophila melanogaster 38,302 Homo sapiens 37,873 Hama sapiens 40,220 Ralstonia eutropha 42.960 Vogesella indligofera 37,626 Caenorhabditis elegans 37,237 Homo sapiens 38,406 14-Jul-99 14-Jul-99 23-Sep-97 10-Sep-99 23-Nov-98 2 1-DEC-1 998 Corynebacterium 99.933 9-Apr-97 glutamnicumn Drosophila melanogaster 36,111 23-Aug-99 Drosophila melanogaster 36,111 23-Aug-99 Daucus carota 39.537 01-MAY-1999 nxa01873 1359 Hama sapiens Mama sapiens Mama sapiens Homo sapiens Mama sapiens Escherichia call 36,419 13-Jul-99 36,317 04-OCT-1999 35,303 22-Aug-99 35,303 22-Aug-99 35,409 8-Sep-99 35,189 26-Apr-93 rxa01922 1275 2006200801 24 Feb 2006 GB-HTG2:AC007853 116280 AC007853 GB-HTG2:AC007853 116280 AC007853 rxa01936 1395 GB-HTG4:AC010037 166249 AC010037 GB-HTG4:AC0iOO37 166249 AC0 10037 GB-PR4:AC005552 167228 AC005552 rxa0l984 420 GB-PR1:HS169C8F 245 Z57239 GBBA1:SERATTBXIS 3255 Li11597 GBEST7:W97557 267 W97557 Table 4 (continued) Drosophila melanogaster chromosome 3 clone BACRO31-O2 (D766) RPCI-98 031L.2 map 9613-96C strain y; cn bw sp, SEQUENCING IN PROGRESS unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO3LO02 (0766) RPCI-98 031L.2 map 9613-96C strain y; cn bw sp, SEQUENCING IN PROGRESS-, unordered pieces.

Drosophila melanogaster chromosome 3L/66B6 clone RPCI98-6E4, SEQUENCING IN PROGRESS 52 unordered pieces.

Drosophila melanogaster chromosome 31166136 clone RPCI98-6E4, SEQUENCING IN PROGRESS 52 unordered pieces.

Homo sapiens chromosome 17, clone hRPK.212 E 8, complete sequence.

H.sapiens CpG island DNA genomic Msei fragment, clone 169c8, forward read cpgl69c8.ftla.

Saccharopolyspora erythraea excisionase (xis) gene. integrase (int) gene, complete cds's and attB site.

mf98a09.rl Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone IMAGE:422296 5, mRNA sequence.

Drosophila melanogaster 34,365 Drosophila melanogaster 34,365 Drosophila melanogaster Drosophila melanogaster Homo sapiens Homo sapiens Saccharopolyspora erythraea Mus musculus 38,534 38,534 36,249 45,679 36,232 42,969 rxa02O6O rxa02O87 1470 GBPR3:AC005544 GBPL1:ATF2OBl8 GB-PL2:ATT25K1 7 rxa02O88 1338 GBHTG3:AC008697 GB-HTG3:AC008697 GBHTG3:AC008703 rxaD2159 636 GBBA2:AF049897 GB-BA2:AFO31 518 GBBA2:AF041436 rxa02184 504 GBBA1:B5Z92953 GBE5T36;Al87807i 169045 104738 89904 167932 167932 213971 9196 2045 516 8164 593 ACD05544 AL049483 ALD49 171 AC00B697 AC008697 AC008703 AF049897 AF03 1518 AF041436 Z92953 A1878071 Homo sapiens chromosome 17, clone hRPK.349_-A_-8, complete sequence.

Arabidopsis thaliana DNA chromosome 4, BAC clone F201318 (ESSA project).

Arabidopsis thaliana DNA chromosome 4, BAC clone (ESSA project).

Homo sapiens chromosome 5 clone CIT978SKB 7003, SEQUENCING IN PROGRESS 54 unordered pieces.

Homo sapiens chromosome 5 clone CIT978SKB_7003, SEQUENCING IN PROGRESS 54 unordered pieces.

H-omo sapiens chromosome 5 dlone CIT978SKB_76P12, -SEQUENCING IN PROGRESS 54 unordered pieces.

Corynebacterium glutamicum N-acetylglutamnylphosphate reductase (argC), H-omo sapiens Arabidlopsis thaliana Arabidlopsis thaliana Homo sapiens Homo sapiens Homo sapiens Corynebacterium 35,724 35,890 38,128 36,662 36,662 34,768 99,843 88,679 100,000 38,951 36,774 2-Aug-99 2-Aug-99 16-OCT-i1999 16-OCT-i1999 26-Nov-98 18-OCT-i1995 6-Jul-94 16-Jul-96 25-Sep-98 (A 24-MAR-i 999 27-Aug-99 3-Aug-99 3-Aug-99 3-Aug-99 1-Jul-98 5-Jan-99 5-Jan-99 24-Jun-98 21-Jul-99 ornithine acetyltransferase (argJ), N-acetylgl uta mate kinase (argB). acetylornithine glutamicum transaminase (argD), ornithine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

Corynebacterium glutamicum ornithine ca rbamolyt ra nsfe rase (argF) gene, Corynebacterium complete cds. glutamicumn Corynebacterium glutamicum arginine repressor (argR) gene, complete cds. Corynebacterium glutamicum B.subtilis yws(A,B,C] genes and rbs[A,C,D,KRJ genes. Bacillus subtilis fc57ai2.yi Zebrafish WashU MPIMG EST Danio rerio cDNA 5 similar to Danio rerio 01Qi3151 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN AO :mRNA sequence.

2006200801 24 Feb 2006 GBE5T37;A1958166 641 A1958 166 rxa02200 1233 GBPR3:HSA494016 GB-HTG2:AC0081 61 GB-HTG2:AC008161 rxa02201 486 GBEST4:H16949 GBEST4:H16949 rxa02202 762 GBIN1:CELC41A3 GB-EST33:AVO801 51 GBGSS5:AQ766877 rxa02205 1002 GBHTG2:AC005959 GBHTG2:AC005959 GBIN1:BRPTUBBA rxa02305 975 GB-RO:MUSPAFR GBPR3:HUMARL1A GBBA1:MLCB2533 rxa02431 899 GBEST4:H35255 GB-HTG1:l-S791K14 GB-HTG1:HS791K14 fxa02446 558 GBBA2:AF036166 GB-EST5:N25122 GBESTS:N25122 rxa02541 1308 GB-BA2:DPU93358 GBEST3O:A1658096 GB-EST37:A1959242 50502 158440 158440 465 465 37149 236 545 127587 127587 4571 1140 1008 40245 407 155318 155318 895 620 620 1267 343 545 ALl 17328 AC008161 AC008161 H16949 H16949 U41541 AV0801 51 AQ766877 AC005959 AC005959 M36380.

050872 L28997 AL03531 0 H35255 AL035685 AL035685 AF0361 66 N25122 N25122 U93358 A1658096 A1959242 Table 4 (continued) fc9lf~l.yl Zebrafish WashU MPIMG EST Danio reria cDNA 5' similar to Danio rerio TR:013151 013151 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN AO ;mRNA sequence.

Human DNA sequence from clone 494016 on chromosome 22, complete Homo sapiens sequence- Mus musculus clone 182_H_5, SEQUENCING IN PROGRESS 29 Mus musculus unordered pieces.

Mus musculus clone 1 82_H_5, -SEQUENCING IN PROGRESS ~,29 Mus musculus unordered pieces.

ym34all1.rl Soares infant brain iNIB Homo sapiens cONA clone IMAGE:50010 Homo sapiens mRNA sequence.

ym34al 11 .rI Soares infant brain 1 NIB Homo sapiens cONA clone IMAGE:5001 0 Homo sapiens mRNA sequence.

Caenorhabditis elegans cosmid C41A3. Caenorhabditis AV080151 Mus musculus stomach C57BLI6J adult Mus musculus cDNA clone Mus musculus 221 04131304, mRNA sequence.

HS 2017B2B08_MR CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=2017 Col=16 Row=D, genomic survey sequence- Homo sapiens, SEQUENCING IN PROGRESS ordered pieces. Homo sapiens H-omo sapiens. SEQUENCING IN PROGRESS ordered pieces. Homo sapiens B.pahangi beta-tubulin gene, complete odls. Brugia pahangi Mouse gene for platelet activating factor receptor, complete cdls. Mus musculus Homo sapiens ARLI mRNA, complete cdls. Homo sapiens Mycobacterium Ieprae cosmid B2533. Mycobacterium ESTi 11890 Rat PC-i 2 cells, NGF-treated (9 days) Rattus sp. cDNA clone Rattus sp.

RPNCOO3, mRNA sequence.

Homo sapiens chromosome 20 clone RP4-791 K1 4, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Homo sapiens chromosome 20 clone RP4-791K14, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Xanthomonas campestris organic hydroperoxide resistance protein (ohr) gene. Xanthomonas complete cds.

yxl 9d1lO.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone Homo sapiens IMAGE:262195 mRNA sequence.

yxl 9dlO.rl Soares melanocyte 2NbHM Homo sapiens cONA clone Homo sapiens IMAGE:262195 mRNA sequence.

Deinocoocus proteolyticus 40 kDa heal shock chaperone protein (dnaJ) gene, Deinococcus complete cdls. proteolyticus fcl4cO9.yi Zebrafish WashU MPIMG EST Danio rerio cDNA 5' similar to Danio redio SW:DNJ2_HUMAN P3 1689 DNAJ PROTEIN HOMOLOG 2. rnRNA sequence.

fd12Shl 1 .yl Zebrafish WashU MPIMG EST Danio redo cONA 5'similar to Danio reria SW:DNJ2_HUMAN P31689 DNAJ PROTEIN HOMOLOG 2. mRNA sequence.

36,774 20-Aug-99 38,648 35,938 35,938 38,267 36,552 elegans 4 1,678 43,348 35,568 40,310 40,310 37,703 38,420 42,188 Ieprae 42,000 39,098 39,456 39,456 ampestris 49,369 35,417 37,172 42,115 52,059 45,438 23-Nov-99 28-Jul-99 28-Jul-99 29-Jun-95 29-Jun-95 08-DEC-1995 25-Jun-99 28-Jul-99 11-Nov-98 11I-Nov-98 C 26-Apr-93 4 1 0-Feb-99 13-Jan-95 27-Aug-99 2-Apr-98 23-Nov-99 23-Nov-99 19-MAY-i1998 28-DEC-i1995 28-DEC-i1995 17-Jan-98 06-MAY-i1999 20-Aug-99 2006200801 24 Feb 2006 Table 4 (continued) DNA encoding Dnak protein which is one of heat shock protein from rxa02542 777 EM-PAT:E10832 1856 E10832 Corynebacterium glutamicum GB-EST24:Z82017 396 GB_OM:CATERYTHRG681 EMPAT: E10832 1856 Z82017 SSZ82017 Porcine small intestine cDNA library Sus scrofa cDNA clone c12c06 5' Sus scrofa similar to eukaryotic initiation factor 4 gamma, mRNA sequence.

L10606 Cat erylhropoietin mRNA, 3Tend. Fetis catus E10832 DNA encoding Dnak protein which is one of heat shock protein from Corynebact glutamicurr 99,000 08-OCT-1997 (Rel. 52, Created) 37,067 30-Apr-99 39,409 14-OCT-1993 97.306 08-OCT-1997 (Rel. 52, Created) rxa02543 1977 erium GBBAI:MPHSP7O 2179 X59437 M.paratubercutosis gene for 70 kD heat shock protein.

GB_BA1:MTY13E1O 35019 Z95324 Mycobacterium tuberculosis H37Rv complete genome; segment 181162.

rxa02586 393 GB_1N2:AC006472 156362 AC006472 GB-ITG4:ACO1002O 106541 AC010020 GBHTG4:AC01 0020 106541 AC01 0020 rxa02587 2214 GBBA1:MLCL622 42498 GB RO:AF074879 3316 GBRO:RNJOO1 380 2641 rxs03217 331 GBBA1:MLCB2548 38916 GBHTG2:HSJ662M14 174772 Z95398 AF074879 AJO01 380 AL023093 AL079336 Drosophila melanogaster, chromosome 2R, region 45E1-46A2, BAC clone BACR48G21, complete sequence.

Drosophila melanogaster chromosome 3LJ66D10 clone RPCI98-2613, SEQUENCING IN PROGRESS 55 unordered pieces.

Drosophila melanogaster chromosome 31J66D1 0 clone RPCI98-2613, SEQUENCING IN PROGRESS 55 unordered pieces.

Mycobacterium Ieprae cosmid L622.

Rattus norvegicus testis-specific protein TSPY gene, complete cdls.

Rattus norvegicus Tspy partial genomic sequence, exons 1-6.

Mycobacterium leprae cosmid B2548.

Homo sapiens chromosome 20 clone RP4-662M14, SEQUENCING IN PROGRESS 10 unordered pieces.

Homo sapiens chromosome 20 clone RP4-662M14, SEQUENCING IN PROGRESS 10 unordered pieces.

Mycobacterium avium 73,404 subsp. paratubercutosis Mycobacterium 72,028 tuberculosis Drosophila melanogaster 37,958 Drosophila melanogaster 37,333 Drosophila melanogaster 37.333 23-Apr-92 1 7-Jun-98 30-Jan-99 16-OCT-i1999 16-OCT-1999 24-Jun-97 6-Jul-99 29-Jun-98 27-Aug-99 4-Feb-00 Mycobacterium teprae Rattus norvegicus Rattus norvegicus Mycobacterium Ieprae Homo sapiens 39,848 35,830 37,702 37,888 36,420 GBHT02:HSJ662M14 174772 AL079336 Homo sapiens 35,962 4-Feb-00 -86- (N Exemplification Example 1: Preparation of total genomic DNA of Corynebacterium glutamicum ATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by 00 Scentrifugation, the supernatant was discarded and the cells were resuspended in 5 ml buffer-I of the original volume of the culture all indicated volumes have been calculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/1 sucrose, C 10 2.46 g/l MgSO, x 7H 2 0, 10 ml/I KH 2 PO, solution (100 g/1, adjusted to pH 6.7 with KOH), 50 ml/1 M12 concentrate (10 g/l (NH,) 2 SO,, 1 g/l NaC1, 2 g/l MgSO, x 7HO, 0.2 g/l CaCI,, 0.5 g/l yeast extract (Difco), 10 ml/l trace-elements-mix (200 mg/1 FeSO, x HO, 10 mg/i ZnSO, x 7 H 2 0, 3 mg/l MnCI, x 4 H 2 O, 30 mg/l H 3 BO, 20 mg/i CoCI, x 6 HO 2 1 mg/l NiCI, x 6 H 2 0, 3 mg/l NaMoO, x 2 HO 2 500 mg/1 complexing agent (EDTA or critic acid), 100 ml/l vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/l p-amino benzoic acid, 20 mg/l riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/l pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37'C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HC1, I mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCI solution (5 M) are added. After adding of proteinase K to a final concentration of 200 plg/ml, the suspension is incubated for ca. 18 h at 37 0 C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing g/ml RNaseA and dialysed at 4 0 C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCI and 0.8 ml of ethanol are added. After a 0 min incubation at -20 0 C, the DNA was collected by centrifugation (13,000 rpm, cI Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in a TE-buffer. DNA prepared by this procedure could be used for all purposes, including southern blotting or construction of genomic libraries.

Example 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC 13032 Using DNA prepared as described in Example 1, cosmid and plasmid O libraries were constructed according to known and well established methods (see ^0 Sambrook, J. et al. (1989) "Molecular Cloning A Laboratory Manual", Cold 0 10 Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons).

Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741); pACYC177 (Change Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene LaJolla USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL109 (Lee, and A.J. Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

Example 3: DNA Sequencing and Computational Functional Analysis Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using AB1377 sequencing machines (see Fleischman, R.D. et al.

(1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496-512). Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3' (SEQ ID NO.

305) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID NO. 306).

Example 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain -88- Sthe integrity of their genetic information. Typical mutator strains have mutations in the genes for the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

S(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM: Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

00 Example 5: DNA Transfer Between Escherichia coli and Corynebacterium 0 Sglutamicum \0 Several Corynebacterium and Brevibacterium species contain endogenous 0 10 plasmids (as pHM1519 or pBL1) which replicate autonomously (for review see, e.g., Martin, J.F. et al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vector into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schifer, A et al.

-89c NI (1990) J. Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for C. glutamicum to E. coli by preparing plasmid DNA from C. glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983) J. Mol. Biol. 166:1-19).

O Genes may be overexpressed in C. glutamicum strains using plasmids which 00 0comprise pCGI Patent No. 4,617,267) or fragments thereof, and optionally the C gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980) 0 Proc. Natl. Acad Sci. USA 77(12): 7176-7180). In addition, genes may be C1 10 overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J Microbiol. Biotechnol. 4: 256-263).

Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

C

(1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer d designed to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is C extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity Sof mRNA for this gene. This information is evidence of the degree of transcription of the 00 OO 0mutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by C several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

S(1992) Mol. Microbiol. 6: 317-326.

C1 10 To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be -91- (1N advantageous to supply mixtures of different carbon sources. Other possible carbon Ssources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials C1 which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NH,CI or NH,OH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast 00 0extract, meat extract and others.

Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, 10 molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121 or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should.be in a range between 15*C and 45"C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It -92r, is also possible to maintain a constant culture pH through the addition of NaOH or SNH,OH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex Scompounds have high buffer capacities. If a fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

The incubation time is usually in a range from several hours to several days. This 00 time is selected in order to permit the maximal amount of product to accumulate in the r broth. The disclosed growth experiments can be carried out in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

N 10 For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 600 of 0.5 1.5 using cells grown on agar plates, such as CM plates (10 g/l glucose, 2,5 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30"C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities may be -93c 1 4found, for example, in the following references: Dixon, and Webb, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San 1 Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 rd ed. Academic Press: New York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 00 3527300325); Bergmeyer, Bergmeyer, GraB1, eds. (1983-1986) Methods of Enzymatic Analysis, 3 r d ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

10 352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C. glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical chromatography such as high performance liquid chromatography (see, for example, -94r, Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: DWeinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al.

c (1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream O processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

00 S(1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, C1 J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of \0 SIndustrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, CN 10 F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall yield, production, and/or efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation.

Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131- 163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C. glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum

C

cells, then the cells are removed from the culture by low-speed centrifugation, and the Ssupernate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to 1 chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the impurities are retained by the resin while the sample is not. Such chromatography steps 00 Smay be repeated as necessary, using the same or different chromatography resins. One Sof ordinary skill in the art would be well-versed in the selection of appropriate Schromatography resins and in their most efficacious application for a particular molecule C 10 to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

EXAMPLE 11: Cloning of a Corynebacterium glutamicum Gene Involved in Lincomycin Resistance Using a Reporter Gene Approach A. Identification of the Gene Encoding the LMRB Protein -96- (11 Plasmid pSL 130 was constructed by ligation of the aceB promoter region S(paceB) of C. glutamicum (Kim, H.J. et al. (1997) J. Microbiol. Biotechnol. 7: 287-292) into the polylinker of the lac operon fusion vector pRS415, which lacks a promoter N, (Simon, R.W. et al. (1987) Gene 53: 85-96). Plasmid pSL145 was constructed by ligating the resulting paceB-lac region into the E. coli cloning vector pACYC 184. E.

coli DH5aF' was transformed with pSL145 and the resulting strain was used as a host 00 0 for screening of a genomic C glutamicum library (in pSL109).

C, Transformants were screened by growth on agar medium containing 5-bromo-4chloro-3-indolyl-beta-D-glalactopyranoside (X-Gal). A white colony, containing DNA 10 influencing lacZ expression, was selected for further analysis. This clone was found to contain a 4 kB fragment from the gene library. Subclones were constructed in pSL109 and a subclone which retained the white phenotype on X-Gal plates was identified. This subclone was found to contain a 2.6 kB BamHI-XhoI fragment (plasmid pSL149-5).

The fragment was sequenced and identified as a membrane protein-encoding gene (LMRB gene).

The 1442 nucleotides of the coding sequence of the LMRB gene encode a polypeptide of 481 amino acid residues with a high percentage ofhydrophobic amino acids. A Genbank search determined that the LMRB protein is 40% identical to the protein product of the ImrB gene from Bacillus subtilis (Genbank Accession AL009126, TREMBL Accession P94422), as determined using a CLUSTAL W analysis (using standard parameters).

The LMRN protein contains a sequence pattern: 158-A-P-A-L-G-P-T-L-S-G-167 (SEQ ID NO:301), which resembles the known multi-drug-resistance-protein consensus motif G-X-X-X-G-P-X-X-G-G (SEQ ID NO:302) (Paulsen, and Skurray, R.A.

(1993) Gene 124: 1-11). Therefore, the LMRB protein was classified as a drug resistance protein.

B. In vivo Analysis of lmrB Function The ImrB gene was overexpressed in C. glutamicum ASO19E12 (Kim, H.J. et al.

(1997) J. Microbiol. Biotechnol. 7: 287-292) using the plasmid pSL149-5, described above.

-97rq Disruption of the LMRB gene was accomplished by use of the vector pSL18lImrB. This vector was constructed as follows: an internal fragment of the LMRB gene was amplified by PCR under standard conditions using primers C CTCCAGGATTGCTCCGAAGG-3' (SEQ ID NO:303) and CACAGTGGTTGACCACTGGC-3' (SEQ ID NO:304). The resulting PCR product 0 was treated with T7 DNA polymerase and T7 polynucleotide kinase, and was cloned 00 0into the SmaI site of plasmid pSL18 (Kim, Y.H. and Lee (1996) J. Microbiol.

0 N Biotechnol. 6: 315-320). The disruption of the LMRB gene in C. glutamicum 0ASO 19E 12 was performed by conjugation, as previously described (Schwarzer and C 10 Puhler (1991) Bio/Technology 9:84-87).

C. glutamicum cells transformed with pSL149-5 displayed similar resistances as untransformed cells against erythromycin, penicillin G, tetracycline, chloramphenicol, spectinomycin, nalidixic acid, gentamycin, streptomycin, ethidium bromide, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and sodium dodecyl sulfate. Significant differences were observed, however, in the resistance of transformed and untransformed cells to lincomycin.

LMRB-overexpressing C. glutamicum cells were found to be able to grow in the presence of 20 jLg/ml lincomycin. In contrast, cells which do not overexpress LMRB (or cells carrying a LMRB disruption) were not able to grow on agar media containing pg/ml lincomycin. This effect was clearly visible in liquid culture. LMRB overexpression led to a 9-fold increased resistance (compared to wild-type) against lincomycin and LMRB disruption resulted in a decreased resistance (28% of wild-type) to this antibiotic.

Example 12: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990)J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100, -98- (N4 wordlength 12 to obtain nucleotide sequences homologous to SRT nucleic acid Smolecules of the invention. BLAST protein searches can be performed with the XBLAST program, score 50, wordlength 3 to obtain amino acid sequences C, homologous to SRT protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped 00 BLAST programs, one of ordinary skill in the art will know how to optimize the C, parameters of the program XBLAST and NBLAST) for the specific sequence

\D

being analyzed.

10 Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4: 11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention were compared to genes present in Genbank in a three-step process. In a first step, a BLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the 99- (N top 500 hits were retained for further analysis. A subsequent FASTA search a Scombined local and global alignment analysis, in which limited regions of the sequences are aligned) was performed on these 500 hits. Each gene sequence of the invention was C subsequently globally aligned to each of the top three FASTA hits, using the GAP program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the

O

0length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 13: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997)Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label may be used to monitor and measure the individual signal intensities of the hybridized molecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic 100- (N acid sample or mixture. DNA microarrays, therefore, permit an analysis of the Sexpression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., Schena, M. (1996) BioEssays 18(5): 427-431).

c The sequences of the invention may be used to design oligonucleotide primers which are able to amplify defined regions of one or more C. glutamicum genes by a nucleic acid amplification reaction such as the polymerase chain reaction. The choice 00 and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the Scovalent attachment of the resulting PCR products to the surface of a support medium Sdescribed above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for example, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray technology, as described above, permits comparative analyses of different strains of C.

101 (C glutamicum or other Corynebacteria. For example, studies of inter-strain variations Sbased on individual transcript profiles and the identification of genes that are important for specific and/or desired strain properties such as pathogenicity, productivity and C stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons of the profile of expression of genes of the invention during the course of a fermentation reaction are possible using nucleic acid array technology.

00 O0 Example 14: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C.

glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et al. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: 1451-1463). Other separation techniques may also be utilized for protein separation, such as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard techniques, such as by staining or labeling. Suitable stains are known in the art, and 102r- include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby S(Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors 35 S-methionine, 35 S-cysteine, 4 C-labelled amino acids, 5 N-amino c acids, "NO 3 or 5

NH

4 or 3 C-labelled amino acids) in the medium of C. glutamicum permits the labeling of proteins from these cells prior to their separation. Similarly, 0 fluorescent labels may be employed. These labeled proteins can be extracted, isolated 00 Sand separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined 10 quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

EQUIVALENTS

Those of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Comprises/comprising and grammatical variations thereof when used in oo Sthis specification are to be taken to specify the presence of stated features, c integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components Sor groups thereof.

Claims (17)

1. 3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.
2. 4. An isolated nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 or SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof. An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.
3. 6. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of Sclaims 1-5 and a nucleotide sequence encoding a heterologous polypeptide.
4. 7. A vector comprising the nucleic acid molecule of any one of claims 1-6. 00 S8. The vector of claim 7, which is an expression vector. O S9. A host cell transfected with the expression vector of claim 8. The host cell of claim 9, wherein said cell is a microorganism.
5. 11. The host cell of claim 10, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.
6. 12. The host cell of claim 9, wherein the expression of said nucleic acid molecule results in the modulation in production of a fine chemical from said cell.
7. 13. The host cell of claim 12, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.
8. 14. A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1. FD C16. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID 00 NO: 302, provided that the amino acid sequence is not encoded by any of the F- C designated genes set forth in Table 1. IO C 17. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, provided that the nucleic acid molecule does not consist of any of the F- designated nucleic acid molecules set forth in Table 1.
9. 18. An isolated polypeptide comprising an amino acid sequence which is at least identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.
10. 19. An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 300, SEQ ID NO: 301 and SEQ ID NO: 302, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence. The isolated polypeptide of any of claims 15-19, further comprising heterologous amino acid sequences.
11. 21. A method for producing a fine chemical, the method comprising culturing the cell of claim 9 such that the fine chemical is produced. D C22. The method of claim 21, wherein said method further comprises the step of recovering the fine chemical from said culture. 00 S23. The method of claim 21, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. IO C 24. The method of claim 21, wherein said cell is selected from the group consisting of: Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, Brevibacterium butanicum, Brevibacterium divaricatum, Brevibacterium flavum, Brevibacterium healii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium linens, Brevibacterium paraffinolyticum, and those strains set forth in Table 3. The method of claim 21, wherein expression of the nucleic acid molecule from said vector results in modulation of production of said fine chemical.
12. 26. The method of claim 21, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.
13. 27. The method of claim 21, wherein said fine chemical is an amino acid. S28. The method of claim 27, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, methionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, I phenylalanine, and tryptophan.
14. 29. A method for producing a fine chemical, the method comprising culturing a cell 00 Swhose genomic DNA has been altered by the introduction of a nucleic acid molecule C of any one of claims 1-6. IO C 30. A method for diagnosing the presence or activity of Corynebacterium diphtheriae in a subject, comprising detecting the presence of at least one of the nucleic acid molecules of any one of claims 1-5 or the polypeptide molecules of any one of claims 15-19, thereby diagnosing the presence or activity of Corynebacterium diphtheriae in the subject.
15. 31. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the nucleic acid molecule is disrupted.
16. 32. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304.
17. 33. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 299, SEQ ID NO: 303 and SEQ ID NO: 304, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule. O 34. A polypeptide produced by the method of claim 14. O A fine chemical produced by the method of any one of claims 21-29. DATED this 2 4 th day of February 2006 BASF AKTIENGESELLSCHAFT oO WATERMARK PATENT TRADE MARK ATTORNEYS 0290 BURWOOD ROAD cl HAWTHORN VICTORIA 3122 O AUSTRALIA cN P20692AU01 seql i st cor r ect ed. t xt SEQUENCE LI STI NG <110> <120> BASF Akt i engesel I schaf t CORYNEBACTERI UM GLUTAM CUM GENES ENCODI NG STRESS, RESI STANCE AND TOLERANCE PROTEI NS <130> BGI -124CPPC <140> PCT/US00/00922 <141> 2000-06-23 <150> 60/141031 <151> 1999-06-25 <150> 60/142692 <151> 1999-07-01 <150> 60/151214 <151> 1999-08-27 <150> DE 19930429.7 <151> 1999-07-01 <150> DE 19931413.6 <151> 1999-07-08 <150> DE 19931457.8 <151> 1999-07-08 <150> DE 19931541.8 <151> 1999-07-08 <150> DE 19932209.0 <151> 1999-07-09 <150> DE 19932230.9 <151> 1999-07-09 <150> DE 19932914.1 <151> 1999-07-14 <150> DE 19940764.9 <151> 1999-08-27 <150> DE 19941382.7 <151> 1999-08-31 <160> 306 <210> 1 <211> 1566 <212> DNA <213> Cor ynebact er i um gl ut ari cum <220> <221> CDS <222> (101)..(1543) <223> RXA01524 <400> 1 ttgtggcact ctttagtagt tttttctcat agctcagttt cgcaacttta gagaactcta gaaactgagc ttcatgctgt gaaaggcctt ttctccattc atg gat tcc caa att 115 Mbt Asp Ser n lie 1 aat act cag acc tct ccg gca gct gcg aag ctg cct agg gag gtc gtt 163 Page 1 Asn Thr G n Thr Ser Pro Ala Al seql i st cor r ect ed. t xt a Ala Lys Leu Pro Arg u gtt Val gaa Gu gt g Val at g let act Thr ttg Leu at c SIle gtt Val at t SIle 150 tct Ser at g let aaa Lys at c SIle ttc Phe 230 gt c Val ct c Leu ctt Leu at t SIle gaa Gu gt g Val acg Thr gcg Al a cag G n 120 ttg Leu t cc Ser gt c Val ccg Pr o at c SIle 200 tcg Ser gca Al a ggc Gy aag Lys at c SIle tcg Ser act Thr at t SIle ttt Phe ttg Leu gtt Val gt g Val gt g Val ctt Leu 170 gtt Val gaa G u ttt Phe ct g Leu at c SIle 250 gac Asp ct c Leu gtt Val gca Al a cca Pr o 75 gtt Val gct Al a ggt G y gtt Val at t SIle 155 aac Asn gtt Val acc Thr gcc Al a gag G u 235 gca Al a aaa Lys gt g Val gcg Al a cag G n act Thr act Thr cca Pr o act Thr cct Pr o 140 tct Ser tct Ser at c SIle aag Lys ttc Phe 220 ggc Gy ct c Leu gca Al a gtt Val ttg Leu 45 tgg Tr p act Thr gcg Al a acg Thr gcg Al a 125 gcg Al a gtt Val ttg Leu gct Al a at c SIle 205 ggt Gy gaa G u ct c Leu ct g Leu t cc Ser cct Pr o ttg Leu ggt Gy ttg Leu ttt Phe 110 ct g Leu gag G u gcg Al a acc Thr ttg Leu 190 acc Thr ggt Gy ggc Gy at c SIle at g Met 270 gcg Al a t cc Ser acc Thr t at Tyr ttg Leu 95 gcg Al a gt g Val cgt Ar g ccg Pr o tgg Tr p 175 gt a Val cca Pr o ttg Leu acc Thr ttt Phe 255 gat Asp at g Met at c SIle act Thr ct g Leu 80 ttc Phe gt g Val at g Met cgt Ar g gcg Al a 160 cac Hi s at t SIle ct g Leu gt g Val gt a Val 240 gct Al a ct c Leu at c SIle at g Met ggc Gy ctt Leu ttt Phe ct g Leu cct Pr o ggt G y 145 ctt Leu tgg Tr p ggt G y gat Asp t ac Tyr 225 ggt Gy ttg Leu cga Ar g at g Met gaa G u ttt Phe gat Asp acg Thr ctt Leu ttg Leu 130 tcg Ser ggt G y ttg Leu ttc Phe gtt Val 210 ggc Gy at c SIle cga Ar g gcc Al a Val Val ctt aat Leu Asn ttc tcc Phe Ser ttg acg Leu Thr ttt tcc Phe Ser ggt acg G y Thr 100 gct cgt Al a Ar g atg acg lMt Thr atg ggc lt G y acg ttg Thr Leu 165 tgg atg Trp let 180 ttg atc Leu II e tct gtc Ser Val agt tcc Ser Ser gcg atc Ala I e 245 cac caa Hi s G n 260 aag gtg Lys Val agg aac ttc agc ttc tcc ttg acc acc atc ctt ttg gcg ttc ggc gcg Page 2 Ar g at g Met gga Gy 310 ct c Leu ggt Gy gca Al a gt g Val cct Pr o 390 cac Hi s gga G y gcg Al a gtt Val t cc Ser 470 s Asn Phe Ser Phe Ser Leu Thr 280 285 eql i st cor r ect ed. t xt Thr Ile Leu Leu Al 29 a Phe G y Al a 0 gga G y act Thr ggt Gy cgt Ar g tcg Ser 360 at g Met at g Met t cc Ser gcg Al a t cg Ser 440 ttc Phe ttc Phe gt c at g Val MVet ttg gtg Leu Val 315 atc agc Ile Ser 330 ctg ct g Leu Leu act ttt Thr Phe gtt gtg Val Val acc gct Thr Al a 395 att ttg Ile Leu 410 atg at t 'e t Ile tct gcg Ser Al a gca ggc Al a G y act cgc Thr Arg 475 gtt Val 300 acc Thr cca Pr o at t SIle ct c Leu ttc Phe 380 ct c Leu aac Asn gca Al a cat Hi s gca Al a 460 ttg Leu ggt Gy ttc Phe ccc Pr o aat Asn 365 agc Ser ggc G y acg Thr gca Al a gct Al a 445 at c l Ie cca Pr o ttg Leu at c SIle gga Gy 350 gag G u at c SIle gcc Al a ttc Phe ctt Leu 430 gaa G u at c SIle at c SIle gtt Val gga Gy 335 gca Al a aat Asn ggc Gy ctt Leu caa G n 415 t cc Ser gct Al a gcg Al a t ac Tyr gtt Val 320 cgt Ar g at t l Ie t ca Ser at g Met ccg Pr o 400 cag G n ttc Phe gtt Val gt g Val ct g Leu 305 at g Met ttc Phe gcg Al a ccc Pr o tgt Cys 385 aag Lys ct c Leu ggc G y gcc Al a at c SIle 465 cag G n ccc Pr o t ac Tyr ct g Leu gt g Val 370 ttg Leu cac Hi s gca Al a act Thr gct Al a 450 gct Al a t cc Ser ggc Gy aag Lys 340 at c SIle at g Met at g Met t at Tyr gca Al a 420 at t SIle acc Thr gtt Val ct c Leu ct c Leu 325 gt c Val gcg Al a gt c Val acc Thr ggt G y 405 gcc Al a gca Al a aag Lys gtt Val 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1553 1566 gtc gag gaa gaa Val u u u gct cac Al a Hi s 480 t aaat accaa aaaatggggc aga <210> 2 <211> 481 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 2 IVet Asp Ser n Ile Asn Thr G n Thr Ser Pro Ala Ala Ala Lys Leu 1 5 10 Pro Arg u Val Val Val Val Leu Ser lie Leu Val Val Ser Ala bet 25 Ile Iet iIe Leu Asn G u Thr Ile Leu Ser Val Ala Leu Pro Ser lie 40 Page 3 seqI i st car r ect ed. t xt [Vbt Ou Asp Phe Ser Val Pro Ou Thr Thr Al a O31n Tr p Leu Thr Thr G31y Le u Ph e Le u Pr o G31y 145 Le u Tr p G31y As p Tyr 225 G31y Le u Ar g Le u Le u 305 vb t Ph e Al a Pr o cys 385 vb t Ar g ValI G31y 115 Le u vb t Pr o Ph e Ph e 1 95 Le u Ph e Ph e O31n Ph e 275 Ph e Thr G31y As p Al a 355 Tr p vb t Thr Ser Thr Ar g Thr G31y Le u 1 65 vb t I Ie ValI Ser I Ie 245 O31n ValI Al a Le u Le u 325 ValI Al a ValI Thr vb t 70 Thr Le u I Ie ValI I Ie 150 Ser b t Ly s I Ie Ph e 230 ValI Le u Ar g vb t G31y 310 Le u G31y Al a ValI Pr o 390 Al a Ly s Thr ValI Thr 135 li1e G31y Le u As n Le u 215 G31y ValI G31y As n Le u 295 ValI O31n Pr a Ser ValI 375 Le u ValI I Ie Al a 1 05 Al a Thr I Ie I Ie I Ie 1 85 O31y ValI I Ie Al a O31n 265 Ser Thr Al a Le u Pr a 345 vb t Hi s Thr Pr a 75 ValI Al a O31y ValI I Ie 1 55 As n ValI Thr Al a O31u 235 Al a Ly s Ser vb t ValI 315 Ser Le u Ph e ValI Al a 395 Thr Al a Thr Al a 125 Al a ValI Le u Al a I Ie 205 O31y O31u Le u Le u Thr 285 Le u O31y Ph e Pr a As n 365 Ser O31y O31y Le u Ph e 110 Le u O31u Al a Thr Le u 1 Thr O31y Oy le vb t 270 Thr Pr a Le u O31y 350 O31u Al a Tyr Le u Al a ValI Ar g Pr a Tr p 1 ValI Pr a Le u Thr Ph e 255 As p I Ie I Ie ValI O31y 335 Al a As n O31y Le u Le u Ph e ValI vb t Ar g Al a 1 Hi s I Ie Le u ValI ValI 240 Al a Le u Le u Tyr ValI 320 Ar g I Ie Se r vb t Pr a 400 Lys His Leu Tyr Oy His Oy Ser Ala Ilie Leu Asn Thr Phe O1n On Page 4 seqI i st car r ect ed. t xt 405 410 415 Leu AlaOy AlaAla (1y Th r Ala Ile I'bt Ile AlaAla Leu Ser Phe 420 425 430 G31y Thr Ser Ilie Al a Al a Ser Ser G31y Ser Al a Hi s Al a G31u Al a Val 435 440 445 Al aAl a 1y Thr Lys Val Al a Phe Ile Al a (1y Al a Ilie Ile Al aVal 450 455 460 Ilie Al a Leu Val Val Ser Leu Phe Val Thr Ar g Val G31u 31u G31u Al a 465 470 475 480 Hi s <210O> 3 <21 1> 371 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (348) <223> RXA00497 <400> 3 t ggaaaccca caaccggcac acacaaaat t t t tct cat gg aac As n g aa (31u aag Ly s tt c Ph e gt t ValI gt g ValI aag Ly s gt c aac at c aag ccg ct t gag Val As n Ilie Lys Pro Leu G31u 10 gca gag acc acc acc gct tcc Al a G3 u Thr Thr Thr Al a Ser 25 gaa aag cca caa gag gca acc G3 u Lys Pr o G31n G3 u Al a Thr 40 gat gac aag ggt aac cgc at c Asp Asp Lys a31y Asn Ar g Ilie gt g at c t tc t cc cgt t ac ggc Val Ilie Phe Ser Ar g Tyr a31y gag t ac t tg ct t ct c t cc gct a3 u Tyr Leu Leu Leu Ser Al a 90 t aggggat aa gt tcat ggca aag g ac As p ggC a31y gt t ValI cca Pr o a31y 75 cgt Ar g at c I Ie gt c ValI gca Al a 45 g ac As p gag a31u at c I Ie agggattcac c gtg gca Val Ala 1 ct c gt t cag atc aac Leu Val a31n I Ie Asn at t cca gat t cc gct Ilie Pr o Asp Ser Al a gtt ggc cca ggc cgc Val a31y Pro a31y Ar g atc aag gaa gat gac Il e Lys a3 u Asp Asp at c aag t tc ggt ggc Il e Lys Phe a3 y a3 y ct c gca at c gt c gag Leu Al a Ilie Val a31u <210O> 4 <21 1> 99 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 4 Page ValI li1e Ser G31y As p G31y ValI As n G31u Ly s Ph e ValI ValI Ly s As n G31u Ly s As p I Ie Tyr Ly s Thr O31n G31y Ser Le u seqI i st car r ect ed. t xt Pro Leu Gu Asp Lys Ilie 10 Thr Al a Se r 03 y Leu Val G31u Al a Th r Val I Ie Al a 40 As n Ar g I Ie Pr o Leu Asp Ar g Ty r 31y G31y Thr G31u Leu Se r Al a Ar g Asp I Ie 90 ValI Pr o G31y Ly s Ly s Al a <210O> <21 1> 1737 <212> DNA <213> Cor ynebact er i urn gi ut ari cumn <220> <221 CIDS <222> 101). (1714) <223> RXN00493 <400> cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gt gacat cct cgcaat cgt c gagaagt agg ggat aagt t c gt act tgct t ct ct ccgct c gct Al a ct g Le u gt t ValI ac c Th r gcg Al a g ac As p ggc (31y g ac As p aac As n g at As p gcc Al a ct g Le u ac c Th r cgc Ar g g ac As p gt c ValI gca Al a g ac As p aag Ly s act Th r gt t ValI gcc Al a aag Ly s t t c Ph e at c I Ie t cc Ser 75 gca Al a gct Al a cgc Ar g gt a ValI G31y g ac As p gt t ValI act Thr gct Al a g aa G31u ac c Thr gga G31y 45 ct t Le u gct Al a ct g Le u ggc (31y ggc G31y ct c Le u cct Pr o gag G31u gt t ValI ct t Le u gca Al a 110 ct c Le u cca Pr o gt c ValI cct Pr o ac c Thr c ag O31n cca Pr o at g vb t 1 cgg Ar g cgc Ar g ac c Thr tt t Ph e aac As n gca Al a at g vb t ggc G31y ggc G31y aac As n gag O31u g ac As p ct c Le u gag (31u gca aag ct c Al a Lys Leu g ac As p aac As n ggt G31y ct c Le u gct Al a gct Al a 1 00 aac As n ggt at t t ct gca gct gca gaa aag acc t tg gaa gag t tg aag gca cgc Page 6 s Gy I e Ser Ala Ala Ala Gu Lys 120 125 eql i st cor r ect ed. t xt Thr Leu G u G u Leu 130 Lys Al a Ar g gca Al a t ca Ser 150 aag Lys gag G u ctt Leu gac Asp gac Asp 230 ct g Leu gt g Val t ac Tyr acc Thr gct Al a 310 gac Asp gca Al a tgg Tr p at t SIle gag Su cgc Ar g ggc G y gct Al a cct Pr o 200 cct Pr o ct c Leu at c SIle t cc Ser ggt Sy 280 gca Al a gaa G u acc Thr cgc Ar g cgc Ar g 360 gt c Val tct Ser gaa G u gac Asp 170 gag G u ttc Phe gt g Val ttg Leu gaa G u 250 cgc Ar g cga Ar g gt c Val gtt Val at c SIle 330 cag G n aag Lys cgc Ar g gac Asp gtt Val 155 ggt G y gt c Val at c SIle ct g Leu ct g Leu 235 gac Asp aag Lys cgc Ar g gt g Val tt c Phe 315 gtt Val at c SIle gca Al a gtt Val acc Thr 140 gt c Val gt c Val acc Thr aac Asn ctt Leu 220 gag G u gt c Val acc Thr aag Lys gat Asp 300 ggt G y gat Asp cgt Ar g gaa G u ggt 30y 380 aag Lys ggc G y gt c Val gaa G u gac Asp 205 gtt Val aag Lys gag G u at c SIle gcg Al a 285 cca Pr o acc Thr ggt G y cgc Ar g gag G u 365 gca Al a gaa G u gag G u acc Thr ggt G y 190 aac Asn cgc Ar g gtt Val ggc G y aag Lys 270 ttc Phe gaa G u gca Al a gca Al a gaa G u 350 cgt Ar g gca Al a gca Al a gtt Val 160 gag G u tct Ser act Thr aag Lys gag G u 240 cct Pr o gtt Val gat Asp ggc G y cgc Ar g 320 t cc Ser gcc Al a gct Al a gaa G u aac Asn 145 gct Al a gag G u ttc Phe cag G n at t SIle 225 t cc Ser ttg Leu gca Al a gac Asp at c SIle 305 at c SIle gca Al a aac Asn aag Lys acc Thr 385 gt c Val gca Al a t cc Ser gac Asp cag G n 210 tct Ser aac Asn cag G n gt g Val ct g Leu 290 aac Asn acc Thr gaa G u acc Thr ct c Leu 370 gaa G u gct Al a gcg Al a cag G n aag Lys 195 gct Al a t cc Ser cgt Ar g acc Thr aag Lys 275 gct Al a ct c Leu gtt Val gac Asp gat Asp 355 t cc Ser gt c Val acc Thr at g Met t cc Ser 180 ggc G y gt c Val ct c Leu cct Pr o ct g Leu 260 t cc Ser at t SIe aac Asn t cc Ser gtt Val 340 t cc Ser ggt G y aac Asn 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca Page 7 Arg Lys Leu Arg Val G u Asp Ala 390 395 eql i st cor r ect ed. t xt lie Asn Ala Ala Arg Ala Ala 400 caa gaa ggc gtt atc gct ggt ggc ggt Sn Gu y Val lle Ala Gy Gy Gy 410 gag act ctg aag gct tac gcc gaa gag G u Thr Leu Lys Ala Tyr Ala G u G u 425 430 ggc gtt cgc gca ctg gct act gct ttg Sy Val Arg Ala Leu Al a Thr Ala Leu 440 445 gcc tcc aac gca ggt ctt gac ggc tct Al a Ser Asn Al a G y Leu Asp G y Ser 455 460 gct ctg cca aac ggc gag ggc ttc aac Ala Leu Pro Asn Gy G u y Phe Asn 470 475 aac ctg atc aac gac ggt gtc atc gac Asn Leu Ile Asn Asp y Val lie Asp 490 gca gta gtg aat gca acc tct gtt gca Ala Val Val Asn Ala Thr Ser Val Ala 505 510 gct tct gtt gtt gag aag cct gca gaa Ala Ser Val Val G u Lys Pro Ala G u 520 525 gga cat cat cac cac taaagttctg tgaal G y Hi s Hi s Hi s Hi s 535 <210> 6 <211> 538 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 6 Iet Al a Lys Leu Ile Al a Phe Asp G n 1 5 Arg y Val Asp Ala Leu Ala Asn Ala 25 Arg y Arg Asn Val Val Leu Asp Lys 40 Thr Asn Asp y Val Thr lie Ala Arg 55 Phe G u Asn Leu G y Al a G n Leu Val 70 Asn Asp Ile Al a G y Asp G y Thr Thr Ala Leu lie Ala Gu y Leu Arg Asn 100 105 IVet u Leu Asn Lys Gy Ile Ser Al a t cc Ser 415 ttc Phe ggc Gy gtt Val gct Al a cca Pr o 495 cgc Ar g gaa Gu gct Al a gaa Gu aag Lys gtt Val gca Al a 480 gt c Val at g Met gca Al a gtt Val gac Asp gcg Al a 450 gca Al a ttg Leu gt c Val ct g Leu gat Asp 530 cag G n cag G n 435 t ac Tyr cgc Ar g gaa Gu ace Thr ace Thr 515 gca Al a at c SIle 420 aag Lys tgg Tr p act Thr t ac Tyr cat Hi s 500 act Thr cat Hi s gct Al a gt c Val at c SIe gct Al a gga Gy 485 t cc Ser gag G u gca Al a 1363 1411 1459 1507 1555 1603 1651 1699 1737 aaacac cgt Asp Ala A 10 Val Lys V Ala Phe C Asp Ile A Lys Ser V Thr Al a T 90 Val Ala A Al a Al a C Page 8 rg 'al Sy sp 'al *hr l a Su G u Thr Gy Leu Al a Leu Gy Lys Gy Leu Pr o G u Val Leu Al a 110 Thr I e Gy Leu Asp Lys Al a Asn Leu seql i st cor r ect ed. t xt 125 Ser Asp Thr Lys u Ile Ala G u Asn 145 Al a G u Phe G n SIle 225 Ser Leu Al a Asp SIle 305 I le Al a Asn Lys Thr 385 Al a Leu Gy Pr o Val 465 Lys Ala Arg Ala Thr u Val Al a Al a G n Lys 195 Al a Ser Ar g Thr Lys 275 Al a Leu Val Asp Asp 355 Ser Val Al a G n G n 435 Tyr Ar g Val G u 165 I le Tyr Leu Pr o Leu 245 Val Pr o Val G u Lys 325 G u Thr Gy Asp Al a 405 Al a Val SIle Al a Ser 150 Lys G u Leu Asp Asp 230 Leu Val Ty r Thr Al a 310 Asp Al a Tr p SIle Ar g 390 G n G u Gy Al a Al a 470 135 Ser Val Thr Ser Asn 215 Phe SIle Asn Phe Lys 295 Gy G u Ar g Asp Al a 375 Lys G u Thr Val Ser 455 Leu Arg Asp G y Lys Al a Leu 185 Pro Tyr 200 Pro Al a Leu Pro I e Al a Ser Ile 265 Sy Asp 280 Al a Thr G u G u Thr Ile Arg G y 345 Arg G u 360 Val lile Leu Arg G y Val Leu Lys 425 Arg Ala 440 Asn Ala Pro Asn Val 155 Gy Val SIle Leu Leu 235 Asp Lys Ar g Val Phe 315 Val SIle Al a Val G u 395 Al a Tyr Al a Leu G u 475 Gy Val G u Asp 205 Val Lys G u SIle Al a 285 Pr o Thr Gy Ar g G u 365 Al a Al a Gy G u Al a 445 Gy Phe G u Thr Gy 190 Asn Ar g Val Gy Lys 270 Phe G u Al a Al a G u 350 Ar g Al a l Ie Gy G u 430 Leu Ser Asn SIle Val 175 I le Asp Asn Val G u 255 Val bet Val Ar g Gy 335 I le Leu Thr Asn Ser 415 Phe Gy Val Al a Val 160 G u Ser Thr Lys G u 240 Pr o Val Asp Gy Ar g 320 Ser Al a Al a G u Al a 400 Al a G u Lys Val Al a 480 Page 9 seqI i st car r ect ed. t xt Thr Leu G31u Tyr Gy Asn Leu lieAsn Asp Gy Val lieAsp Pro Val 485 490 495 Lys Val Thr Hi s Ser Al a Val Val Asn Al a Thr Ser Val Al a Ar g I'b t 500 505 510 Val Leu Thr Thr 31u Al a Se r Val Val G31u Lys Pro Al a 31u G31u Al a 515 520 525 Al a Asp Al a Hi s Al a 03 y Hi s Hi s Hi s Hi s 530 535 <210> 7 <211> 1339 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221> CIDS <222> (1339) <223> FRXA00498 <400> 7 cccgt t acgg cggcaccgag at caagt t cg gt ggcgt gga gt act t gct t ct ct ccgct c gt gacat cct cgcaat cgt c gagaagt agg ggat aagt t c at g gca aag ct c at t I'bt Al a Lys Leu I I e gct Al a ct g Le u gt t ValI ac c Thr gcg Al a g ac As p ggc G31y ggt G31y gca Al a g ac As p aac As n g at As p gcc Al a ct g Le u ac c Thr cgc Ar g t ct Ser 120 gag (31u g ac As p gt c ValI gca Al a g ac As p aag Lys5 act Thr gt t ValI gct Al a t ct Ser gcc Al a aag Lys5 t t c Ph e at c SI e t cc Ser 75 gca Al a gct Al a gca Al a gac As p cgc Ar g gt a ValI G31y g ac As p gt t ValI act Thr gct Al a g aa G31u ac c Thr 140 g aa G31u ac c Thr gga G31y 45 ct t Le u gct Al a ct g Le u ggc G31y aag Lys5 125 aag Lys5 ggc G31y ct c Le u cct Pr o gag G31u gt t ValI ct t Le u gca Al a 110 ac c Thr g aa (31u ct c Le u cca Pr o gt c ValI cct Pr o ac c Thr C ag (31n cca Pr o g aa G31u gca Al a ggc G31y ggc G31y aac As n gag G31u g ac As p ct c Le u gag G31u tt g Le u 130 gt c ValI gt t ValI cgt Ar g g ac As p aac As n at c I Ie at t I Ie ct c Le u 115 aag Lys5 gct Al a g ac As p aac As n ggt G31y ct c Le u gct Al a gct Al a 1 00 aac As n gca Al a ac c Th r t ca t cc cgc gat gaa gt t gt c ggc gag Ser Ser Arg AspGu Val Val Gy Gu at c gt t gct gca gcg at g gaa Ilie Val Ala Ala Ala I'bt Gu Page seql i st cor r ect ed. t xt 150 aag Lys gag G u ctt Leu gac Asp gac Asp 230 ct g Leu gt g Val t ac Tyr acc Thr gct Al a 310 gac Asp gca Al a tgg Tr p at t SIle cgc Ar g 390 caa G n ggc Gy gct Al a cct Pr o 200 cct Pr o ct c Leu at c SIle t cc Ser ggt Gy 280 gca Al a gaa Gu acc Thr cgc Ar g cgc Ar g 360 gt c Val ct g Leu ggc Gy gac Asp 170 gag G u ttc Phe gt g Val ttg Leu gaa G u 250 cgc Ar g cga Ar g gt c Val gtt Val at c SIle 330 cag G n aag Lys cgc Ar g gt c Val at c SIle 410 ggt Gy gt c Val at c SIle ct g Leu ct g Leu 235 gac Asp aag Lys cgc Ar g gt g Val tt c Phe 315 gtt Val at c SIle gca Al a gtt Val gaa G u 395 gct Al a gt c Val acc Thr aac Asn ctt Leu 220 gag G u gt c Val acc Thr aag Lys gat Asp 300 ggt Gy gat Asp cgt Ar g gaa G u ggt Gy 380 gat Asp ggt Gy 160 gtc acc gtt gag Val Thr Val G u 175 gaa ggt att tct G u G y I e Ser 190 gac aac gac act Asp Asn Asp Thr 205 gtt cgc aac aag Val Arg Asn Lys aag gtt gtg gag Lys Val Val G u 240 gag ggc gag cct Su Gy u Pro 255 atc aag gtc gtt Ile Lys Val Val 270 gcg ttc atg gat Al a Phe IVet Asp 285 cca gaa gtg ggc Pro u Val y acc gca cgc cgc Thr Ala Arg Arg 320 ggt gca ggt t cc G y Al a y Ser 335 cgc gaa atc gcc Arg u lie Ala 350 gag cgt ttg gct G u Arg Leu Ala 365 gca gca act gaa Ala Ala Thr G u gcc atc aac gct Ala lle Asn Ala 400 ggc Gy t cc Ser gac Asp cag G n 210 tct Ser aac Asn cag G n gt g Val ct g Leu 290 aac Asn acc Thr gaa G u acc Thr ct c Leu 370 gaa G u t cc Ser 180 ggc Gy gt c Val ct c Leu cct Pr o ct g Leu 260 t cc Ser at t SIle aac Asn t cc Ser gtt Val 340 t cc Ser ggt Gy aac Asn at c SIe t ac Tyr ct g Leu cca Pr o ttg Leu 245 gtt Val cct Pr o gt c Val gaa G u aag Lys 325 gaa G u acc Thr ggt Gy gac Asp gca Al a 405 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1339 gct cgc gca gca Ala Arg Ala Ala <210> 8 Page 11 seql i st cor r ect ed. t xt <211> 413 <212> PRT <213> Corynebact eri um gl ut am cum <400> 8 Mbt Ala 1 Arg G y Arg G y Thr Asn Phe G u Asn Asp Al a Leu Mvt G u G u Leu 130 Asn Val 145 Al a Al a Su Ser Phe Asp G n Gn 210 Ile Ser 225 Ser Asn Leu G n Al a Val Asp Leu 290 Ile Asn 305 Lys Leu Ile Al a Val Ar g Asp Asn SIle SIle Leu 115 Lys Al a Al a G n Lys 195 Al a Ser Ar g Thr Lys 275 Al a Leu 5 Al a Val Val Gy Gy G u Lys Ar g Val G u 165 I le Tyr Leu Pr o Leu 245 Val Pr o Val G u Lys 325 Leu Val Thr Al a 70 Asp Gy Gy Al a Ser 150 Lys G u Leu Asp Asp 230 Leu Val Ty r Thr Al a 310 Phe Al a Leu SIle G n Gy Leu SIle Thr 135 Ser Val Thr Ser Asn 215 Phe SIle Asn Phe Lys 295 Gy Asp Asn Asp 40 Al a Leu Thr Ar g Ser 120 G u Ar g Gy Al a Pr o 200 Pr o Leu SIle Ser Gy 280 Al a G u n Al a Lys Ar g Val Thr Asn 105 Al a Val Asp Lys Leu 185 Tyr Al a Pr o Al a SIle 265 Asp Thr G u Asp 10 Val Al a Asp Lys Thr 90 Val Al a Ser G u Asp 170 G u Phe Val Leu G u 250 Ar g Ar g Val Val Al a Lys Phe SIle Ser 75 Al a Al a Al a Asp Val 155 Gy Val SIle Leu Leu 235 Asp Lys Ar g Val Phe 315 u Thr Gy Leu Al a Leu Gy Lys 125 Lys Gy Val G u Asp 205 Val Lys Su I e Al a 285 Pr o Thr Gy Leu Pr o G u Val Leu Al a 110 Thr G u G u Thr Gy 190 Asn Ar g Val Gy Lys 270 Phe a u Al a I e Leu G y Pro Leu Val Asp Pro Lys Thr Al a n Asn Pro Leu G u I e Al a Ile Val 160 Val G u 175 lie Ser Asp Thr Asn Lys Val G u 240 G u Pro 255 Val Val IVet Asp Val G y Arg Arg 320 G y Ser 335 Ile Thr Val Ser Asp u Thr Ile Ile Val 330 Asp G y Al a Page 12 seql i st cor r ect ed. t xt Al a u Asp Val u Ala Arg Arg G y Gn I e Arg Arg Gu I e Ala 340 345 350 Asn Thr Asp Ser Thr Trp Asp Arg G u Lys Ala G u G u Arg Leu Ala 355 360 365 Lys Leu Ser Gy Gy Ile Ala Val Ile Arg Val y Ala Ala Thr u 370 375 380 Thr G u Val Asn Asp Arg Lys Leu Arg Val G u Asp Ala lie Asn Ala 385 390 395 400 Ala Arg Ala Ala Ala n G u G y Val IIe Ala G y G y 405 410 <210> 9 <211> 723 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(700) <223> RXA01217 <400> 9 gcgctgcctg cgaagactcg accaagacat tcgagtcggt cg gcggccgagt gtccacct t c atccatgagg agaaatcact at cgggcatt ttttattttc g gca aag tac caa acc Thr cgt Ar g gtt Val ct g Leu ggc G y acc Thr aag Lys ggc G y gag Gu cgc Ar g t cc Ser cgc Ar g aag Lys cac Hi s gag G u at g Met 120 gct Al a gct Al a ct g Leu gaa Gu ct c Leu gac Asp gac Asp gtt Val cgc Ar g cag G n gt c Val gt a Val at g Met ttg Leu cca Pr o gat Asp tct Ser at c SIle acc Thr 45 aac Asn at c SI e gac Asp gtt Val gct Al a 125 gag G u cct Pr o at c SIle gct Al a aag Lys ct g Leu at c SIle 110 gac Asp ttc Phe 15 gct Al a gac Asp gtt Val cac Hi s ctt Leu 95 gt c Val acc Thr ggc Gy gtt Val cac Hi s ctt Leu at c SIle 80 gcc Al a gag G u at c SIle IVet Ala Lys Tyr 1 aag ggc tct gca Lys G y Ser Al a gtt tac ggc gca Val Tyr G y Al a cgc acc ttc gct Arg Thr Phe Ala gag ctc gac atc G u Leu Asp Ile gac cag aac gtg Asp G n Asn Val att aag cgc ggc lle Lys Arg y 100 ggc gag cca gct G y G u Pro Al a 115 aag gtt gag gct Lys Val G u Ala 130 cgt Ar g gat Asp gcg Al a gag G u ctg Leu gaa G u cca Pr o gac Asp gtt ctg tcc atc cct gaa gag ttc acc gtt tcc atc gaa ggc ctt gag Val Leu Ser lie Pro G u G u Phe Thr Val Ser lie G u Gy Leu u Page 13 seqI i st car r ect ed. t xt ctc ggc gca cag atc acc gca gct gac atc aag ctc gag ggc gac acc Leu G3 y Al a G3 n Il e Thr Al a Al a Asp Il e Lys Leu G3 u G3 y Asp Thr 150 155 160 165 acc ct g gt t gag gat cct gag acc ct c at c gt c aac at c gt t ct c cca Thr Leu Val a31u Asp Pr o a31u Thr Leu Ilie Val Asn Ilie Val Leu Pr o 170 175 180 gct gt c gag gaa gaa gac acc gaa gag gac gaa gca gct gaa gaa gca Al aVal a31ua(1ua1u Asp Thr a31ua1u Asp(1u Al aAl aa(1ua1u Al a 185 190 195 gct act gag t aagctt t tt t agat agctt t at Al a Thr a3 u 200 <210O> <21 1> 200 <21 2> PRT <213> Corynebacter <400> iurn gi ut ani curn vb t Ly s ValI Ar g a31u As p I Ie a31y Ly s I Ie 145 Le u As n Al a Al a Lys Tyr a3 y Se r AlE 2C Tyr a3 y AlE Thr Phe Al E Leu Asp liE a3 n Asn Val Lys Ar g a31 1 OC a31u Pr o Al E 115 Val a31u Al E 130 a3 a 3 y LeL a31ua1y Asr Ilie Val LeL 1 8C Al a a3 u a3 1 a31n Th r I Ie a31u Al a Ar g As p Al a a31u Le u a31u Pr o As p a31u Thr 1 65 Pr o Al a Ar g ValI Le u a31y 70 Thr Ly s a31y ValI Le u 150 Thr Al a Al a Al a a31u ValI 55 a31n Ph e ValI Thr Le u 135 a31y Le u ValI Thr ValI As n a31n a31n I Ie ValI 1 Tr p I Ie a31n a31u a31u 1 Al a 10 Al a Le u a31u Le u As p 90 As p ValI Pr o I Ie As p 1 70 a31u ValI a31y Hi s a31y Thr Hi s ValI a31n a31u Thr 1 Pr o As p Ar g a31n ValI ValI vb t Le u Pr a As p a31u 140 Al a a31u Thr Ser I Ie Thr As n I Ie As p ValI Al a 125 Ph e Al a Thr a31u Ph e Al a As p ValI Hi s Le u ValI Thr ValI I Ie I Ie 1 As p <210O> 11 <21 1> 1603 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn Page 14 seql i st cor r ect ed. t xt <220> <221> CDS <222> (33)..(1580) <223> RXA00605 <400> 11 ataggtaacc ctcataaaag gaaggaatgc ta gac Asp acc Thr gca Al a aag Lys gca Al a t cc Ser ct g Leu acc Thr 120 ggc G y gac Asp aag Lys gca Al a acc Thr 200 cag G n at c I e cac Hi s cca Pr o gca Al a ggc G y t ac Tyr gtt Val cgc Ar g t ac Tyr at g Met ggt Gy 170 gaa G u aag Lys at c SIle gat Asp gga Gy ggt Gy ttt Phe ggc G y aag Lys ttc Phe gt c Val at c SIle 140 ttc Phe cgc Ar g gca Al a t cc Ser gct Al a 220 cgc Ar g gca Al a cca Pr o 45 aac Asn gct Al a gca Al a tct Ser cac Hi s 125 gt g Val ccg Pr o gat Asp cac Hi s cgc Ar g 205 gaa G u gga Gy cca Pr o aac Asn cgc Ar g ttc Phe gac Asp act Thr 110 ggc G y ggt G y gac Asp gca Al a cag G n 190 cac Hi s ggt Gy cgt Ar g cca Pr o ct c Leu aac Asn gag G u ttc Phe gct Al a gct Al a aac Asn at c SIle 160 at g Met acc Thr gac Asp cca Pr o at g Met 1 cca Pr o tct Ser aat Asn gtt Val 65 ct g Leu cag G n ggt G y ctt Leu acc Thr 145 cac Hi s cag G n t ac Tyr ggc G y gtt Val 225 tct gag aag Ser G u Lys t ca gca gca Ser Al a Al a aag Lys gag G u gac Asp 50 cca Pr o cac Hi s cct Pr o gag G u cgc Ar g 130 cca Pr o t ca Ser tgg Tr p ct g Leu ttc Phe 210 tgg Tr p tct Ser at c SIle cac Hi s cgt Ar g acc Thr aag Lys 100 ggc G y t ac Tyr ttc Phe aag Lys ttc Phe 180 ggt G y t cc Ser aag Lys gga G y t cc Ser ct c Leu at c SIle gag G u gt c Val t cc Ser acc Thr ttc Phe cgt Ar g 165 tgg Tr p gac Asp cac Hi s t ac Tyr aac act Asn Thr gca acc Al a Thr att gaa Ile u cct cac Pro His gac gta Asp Val acc ccg Thr Pro cca gat Pro Asp gaa gag G u G u 135 ctt cgt Leu Arg 150 ctc aac Leu Asn acc cgc Thr Arg cgc ggt Arg G y acc ttc Thr Phe 215 cac ttc Hi s Phe 230 aag acc cgc cag ggc tgg gat tgc ttc acc gat gca gaa gca gca aag Page Lys Thr Arg G n y Trp Asp Cys 235 eql i st cor r ect ed. t xt Phe Thr Asp Ala G u Al a Al a Lys gtt Val at t SIle cct Pr o 280 aag Lys at c SIle gca Al a cgc Ar g cgc Ar g 360 gag G u gct Al a ggc G y ggc Gy ct g Leu 440 cag G n cga Ar g acc Thr ggc G y 250 aac Asn gag G u tgg Tr p aac Asn gat Asp 330 ct c Leu ggc G y aac Asn ggc G y ct g Leu 410 gct Al a cgt Ar g ggc Gy gca Al a cca Pr o 490 aac Asn gac Asp gca Al a cag G n 300 aac Asn ggc Gy gca Al a aac Asn t ac Tyr 380 cct Pr o aac Asn gac Asp gct Al a ct a Leu 460 aac Asn gt c Val gct Al a tt c Phe gag G u 285 aag Lys cca Pr o aac Asn cgt Ar g tac Ty r 365 agc Ser t cc Ser ggt G y at c SIle tac Ty r 445 tac Ty r at c SIle tac Ty r gac Asp cca Pr o 270 aac Asn gat Asp cgc Ar g at c SIle at c SIle 350 cgc Ar g cgc Ar g t ac Tyr acg Thr t ac Tyr 430 gt c Val cgc Ar g t cc Ser gac Asp t ac Tyr 255 at c SIle t ac Tyr t ac Tyr aac Asn gtt Val 335 ttc Phe gac Asp gaa G u agc Ser gat Asp 415 gt c Val aag Lys gag G u aac Asn t ac Tyr 495 240 cag G n tgg Tr p cgc Ar g cca Pr o ttc Phe 320 cct Pr o gca Al a ct g Leu ggt G y cct Pr o 400 t cc Ser aac Asn cac Hi s gt c Val gca Al a 480 tgg Tr p cgc Ar g gac Asp tgg Tr p ct g Leu 305 ttc Phe ggc Gy t ac Tyr cca Pr o t cc Ser 385 aac Asn t cc Ser cca Pr o cag G n ct g Leu 465 at g Met aac Asn gaa G u gt c Val aac Asn 290 at c SIle gct Al a gt c Val gct Al a gt g Val 370 at g Met cgc Ar g t cc Ser gac Asp gat Asp 450 gat Asp cag G n aac Asn gac Asp aag Lys 275 cca Pr o cca Pr o cag G n ggc G y gac Asp 355 aac Asn cag G n t ac Tyr aac Asn cca Pr o 435 gat Asp gag G u ggc G y gtt Val ct c Leu 260 gtt Val ttc Phe gt c Val at c SIle ct g Leu 340 cag G n cgt Ar g t ac Tyr gac Asp cac Hi s 420 cac Hi s gac Asp ggc G y at c SIle gat Asp 500 aac Asn at c SIe ct g Leu t ac Tyr 310 cag G n cca Pr o cgt Ar g at c SIe ttc Phe 390 ggc G y t cc Ser acc Thr ttc Phe aag Lys 470 gag G u aac Asn 821 869 917 965 1013 1061 1109 1157 1205 1253 1301 1349 1397 1445 1493 1541 1590 ggc gct cgc gtc aag gag ctt tac ctc cag aag aag gct taagtccttc Page 16 seqi i st car r ect ed. t xt O31y Ala Ar g Val Lys O31u Leu Tyr Leu O31n Lys Lys Ala 505 510 515 t gat t taaaa t ga <210O> 12 <211> 516 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1 603 <400> 12 l'bt Ser 03 u Lys Ser A a A a Asp 03 n Pr o Ser As n ValI Le u O31n O31y Le u Thr 145 Hi s O31n Tyr G31y ValI 225 Thr Ar g As p Le u As n li1e (31u I Ie G31y O31n 115 Ph e Thr O31n As p vb t 1 95 O31y ValI A a As p Ly s 275 O31y Ser Le u I Ie O31u ValI Ser Thr Ph e Ar g 1 65 Tr p As p Hi s Tyr A a 245 Tyr O31n As n A a I Ie Pr o 70 As p Thr Pr o O31u Le u 150 Le u Thr Ar g Thr Hi s 230 A a As n I Ie Thr Thr O31u Hi s ValI Pr o As p O31u 135 Ar g As n Ar g O31y Ph e 215 Ph e Ly s A a vb t Thr A a 40 Ly s A a Ser Le u Thr 120 O31y As p Ly s A a Thr 200 O31n Ly s ValI I Ie Pr a 280 Ar g O31y Le u Ly s O31u A a 1 Tr p As n O31y As n Pr a 1 Pr a Tr p Thr A a O31u 265 Ph e ValI As n O31n Hi s Hi s 75 Thr Ar g As p As p Ly s 1 55 Le u Ser Thr As n O31n 235 O31u O31y As p As p O31y O31y Ph e O31y Ly s Ph e ValI I Ie 140 Ph e Ar g A a Ser A a 220 O31y As n As p A a Ar g A a Pr a As n A a A a Ser Hi s 125 ValI Pr a As p Hi s Ar g 205 O31u Tr p A a Ph e O31u 285 vb t ValI ValI O31u O31y Le u ValI Ph e As n Ph e As p 1 ValI O31n Ly s cys Tyr 255 li1e Tyr Ar g Pr a Le u As n O31u Ph e A a A a As n I Ie 1 vb t Thr As p Pr a Ph e 240 O31n Tr p Ar g Tr p Asn Pr o Phe Asp Leu Thr Lys Thr Tr p Ser (31n Lys Asp Tyr Pr o 290 295 300 Page 17 seqI i st car r ect ed. t xt Leu Ilie Pro Val Gy 305 Phe Il e Leu Asn Ph e G31y Tyr Pr o Ser 385 As n Ser Pr o O31n Le u 465 vb t As n O n O31n G31y As p 355 As n O31n Tyr As n Pr o 435 As p O31u O31y ValI Ly s 515 O31u 325 Ser O31n Pr o I Ie Ly s 405 Thr G y As p O31u Ser 485 O31u Le u As p Tyr As n 375 As p Al a Tyr As p I Ie 455 O31u Al a Le u Al a Ar g Ar g 360 O31u Al a O31y O31y Le u 440 O31n Ar g Thr O31y Le u vb t 345 li1e ValI O31u Tyr O31n 425 ValI Pr a Le u O31u Al a 505 As p 330 Le u O31y As n O31y Le u 410 Al a Ar g O31y Al a Pr a 490 Ar g Ar g 315 Pr a O31n Al a Thr O31u 395 As p As p Al a I Ie As p 475 Ar g ValI Asn Pro Arg Asn O31y Al a As n Tyr 380 Pr a As n As p Al a Le u 460 As n ValI Ly s As n Ar g Tyr 365 Ser Ser Oy le Tyr 445 Tyr I Ie Tyr O31u ValI 335 Ph e As p O31u Ser As p 415 ValI Ly s O31u As n Ty r 495 Tyr <210O> 13 <21 1> 2439 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (2416) <223> RXA00404 <400> 13 aagat ccgat cat cggcat a cagaaacacc cat ct ggccg aact t t cct t t t t ct gcat g cat t tct gca cacagt t tct gcccgct gt t t ct acgcat a gt g gct t tg aaa cga 115 Val Al a Leu Lys Arg 1 ccc gaa gag aaa aca gt a aag at c gt g acc at a aaa cag act gac aac 163 Pro Ou Ou Lys Thr Val Lys I Ie Val Thr Ilie Lys O31n Th r Asp Asn 15 at c aat gac gat gat t tg gt g t ac agc aac gct act gac ctt cca gt a 211 Ilie Asn Asp Asp Asp Leu Val Tyr Ser Asn Ala Thr Asp Leu Pro Val 30 Page 18 seql i st cor r ect ed. t xt ggc Gy ctt Leu t cc Ser ggt Sy aaa Lys aat Asn cac Hi s at g Met 150 ggt Gy tct Ser at g Met at g Met gct Al a 230 gca Al a act Thr gct Al a tgg Tr p aag Lys ttt Phe ggc G y t at Tyr gtt Val ggc G y 120 ttt Phe gcg Al a at t SIle cgt Ar g acg Thr 200 at c SIle t ca Ser ttc Phe gt g Val ggt Sy 280 aag Lys aag tcc cct Lys Ser Pro ggg gt t at c G y Val lie gaa aca at c Su Thr Ile 75 atc att gcg Ile Ile Al a aag ccg aaa Lys Pro Lys 105 aag gac tat Lys Asp Tyr gca gct at t Al a Al a I le cag atg ggc G n Iet y 155 ttc gcc ggt Phe Al a G y 170 agg cgt gga Arg Arg G y 185 gtc ggt gga Val Gy y att atc gcg Ile Ile Al a cca t g ggc Pro Trp G y 235 atg ggt gtg MVt G y Val 250 t cc atc at c Ser Ile lle 265 tgg gtt gca Trp Val Al a acc act ttg Thr Thr Leu aaa at g t ca Lys Vet Ser 45 gcg gcg gtg Al a Al a Val aac tct gt g Asn Ser Val ttt tct ttc Phe Ser Phe gat cag cga Asp n Arg 110 gt c cca acg Val Pro Thr 125 gca ggt gcc Al a G y Al a 140 tac ctg cca Tyr Leu Pro gca gtg cag Ala Val n cgc tca ct t Arg Ser Leu 190 gct gcc ggt Al a Al a G y 205 gtg ctc gca Val Leu Al a 220 gtt ttc tcc Val Phe Ser tac ttg cgt Tyr Leu Arg ggt gtg gca G y Val Al a 270 gac acc t ca Asp Thr Ser 285 gcg ttg gcc Ala Leu Al a 300 ccg acc gc Pro Thr Al tgg Tr p ct g Leu gcc Al a acc Thr cgt Ar g cca Pr o acc Thr 160 t ac Tyr cag G n ttg Leu at c l Ie acc Thr 240 ct g Leu ct c Leu ggc G y gg 6 gt Va cg Ar cc Pr cg Ar tt Le 14 tt Le ct Le at Me gc Al gt Va 22 at Me cg Ar ct Le gt Va c cgc gtt a Arg Val a gca at c y Ala lle t ttg gcg I Leu Ala a ctg at t g Leu Ile g gcg gaa o Al a G u 115 t gtg ctt g Val Leu 130 g gtt gga u Val y g tgg att u Trp Ile a gtg ct g u Val Leu g gtt cgt t Val Arg 195 g acc at c a Thr Ile 210 g gtt aat I Val Asn g acc at c t Thr Ile c cca ggt g Pro y g ctg gct u Leu Al a 275 g gaa tgg l G u Tr p 290 ggt Gy gct Al a gca Al a gaa G u 100 t ac Tyr ttt Phe cct Pr o at c SIe tgg Tr p 180 gat Asp t cc Ser gca Al a cca Pr o cgt Ar g 260 at c SIe ttc Phe 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 ttg atc ggt Leu I e G y 305 Page 19 tac gga at c at g Tyr y l e Met seql i st cor r ect ed. t xt gct Al a 310 t ct Ser ttg Leu ctt Leu ttc Phe tct Ser 390 at g Met gca Al a aac Asn tgg Tr p ct g Leu 470 acc Thr gga G y at c SIle cgt Ar g ct g Leu 550 at t l Ie ttt Phe gca Al a ggc G y 360 acg Thr gga G y ggc Gy at c l Ie ccg Pr o 440 aac Asn gaa Gu ggc G y at c SIle ttt Phe 520 gct Al a cgt Ar g ccg Pr o aag Lys 330 cct Pr o ggt Sy gcc Al a aca Thr ggc Gy 410 gct Al a gca Al a at t SIle gct Al a cca Pr o 490 ggc G y gct Al a ttt Phe aag Lys tgt Cys 570 gtg tgg Val Trp 315 at c ggc i e G y gag gt g G u Val ccg gtg Pro Val tgt ggt Cys G y 380 cca aag Pro Lys 395 ggc atg G y let gtt att Val lile ct g act Leu Thr ggg ctg G y Leu 460 gaa agt Su Ser 475 acc ttg Thr Leu gct gga Al a G y ctg ttc Leu Phe at g at g bet bet 540 gat cct Asp Pro 555 ctg ctg ctt Leu Leu Leu gt c Val cag G n ttc Phe 365 gca Al a ctt Leu ttg Leu ct g Leu ggt G y 445 aca Thr gt c Val gcg Al a at g Met at c SIle 525 acc Thr t ca Ser at c SIle at g Met 350 tct Ser ct g Leu gt g Val at g Met gat Asp 430 gga G y ggt G y gga G y ttc Phe aag Lys 510 ct c Leu gat Asp tgg Tr p ggt G y 335 cct Pr o gga G y tct Ser gag G u gaa G u 415 cgt Ar g gat Asp gcg Al a gaa G u ggt G y 495 gcg Al a act Thr acc Thr act Thr gct Al a 575 gca Al a 320 ct g Leu t cc Ser agt Ser ggt G y aag Lys 400 tct Ser cac Hi s cca Pr o gat Asp t cc Ser 480 at g Met ttc Phe act Thr ttg Leu gt c Val 560 at t l Ie ccg cgc gat Pro Arg Asp ttg gca gtg Leu Al a Val gt g acc t cc Val Thr Ser 355 ctg ttc cca Leu Phe Pro 370 ttc cac gca Phe Hi s Al a 385 gaa t cc cag Su Ser G n ttc gtg gcg Phe Val Al a ctg tac ttc Leu Tyr Phe 435 gca acc gca Al a Thr Al a 450 atc acc ccg I e Thr Pr o 465 act gtt at t Thr Val I le t ct gaa at c Ser G u Ile tgg tac cac Trp Tyr Hi s 515 gtg gat gca Val Asp Al a 530 ggc aat gtt G y Asn Val 545 ggt aac t gg Sy Asn Tr p ttg ctc atg Leu Leu I\et t ac Tyr ggt G y 340 ttc Phe ttc Phe ct g Leu at g Met at g Met 420 t cc Ser gct Al a gaa G u t cc Ser ct c Leu 500 ttc Phe ggt G y cca Pr o at t SIe ggt Gy 580 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 acc gtg ttt gtg Thr Val Phe Val gct cta tgg ggt Al a Leu Trp G y Page seql i st cor r ect ed. t xt acc Thr gct Al a gt g Val ttg Leu 630 ttc Phe cgc Ar g cct Pr o ttg Leu at t SIle 710 ttg Leu act Thr gac Asp cca ct g Pro Leu 585 cag ctg G n Leu 600 aag aag Lys Lys tgg gat Trp Asp tct gat Ser Asp gca aag Al a Lys 665 gca at c Ala I e 680 at c ct g I e Leu gtg tgc Val Cys acc act Thr Thr ttc ctt Phe Leu 745 cgc tac Arg Tyr 760 ggc ggc atc aac gtg ctt Gy Gy lie Asn Val Leu 590 ctc gcc gct att gca ctt Leu Al a Ala Ile Ala Leu 605 ggc ctg tac aag tgg gcg Gy Leu Tyr Lys Trp Ala 620 ctc att gtc acg atg act Leu Ile Val Thr bet Thr 635 ccg gct att ggc tac tgg Pro Al a lie y Tyr Trp 650 655 tct caa ggc ctt acc gaa Ser G n G y Leu Thr G u 670 gat gcg gtt atc cga aac Asp Al a Val IIe Arg Asn 685 ttc gcg gtg ctc gtc ctc Phe Al a Val Leu Val Leu 700 atc aag tcc atc agg gct Ile Lys Ser Ile Arg Ala 715 gaa gag cct gat act gaa G u G u Pro Asp Thr u 730 735 gca tct tcc agg gat aag Ala Ser Ser Arg Asp Lys 750 cca ggc ggt gcg ccc gtg Pro y y Ala Pro Val 765 ttc cca cta ttc Phe Pro Leu gct Al a tgg Tr p gcg Al a 640 gct Al a ttt Phe acc Thr gtt Val cgt Ar g 720 tct Ser gaa G u tct Ser ct c Leu at t SIle 625 t ca Ser cag G n ggt G y at g Met gtt Val 705 gca Al a gag G u gt c Val tct Ser gt g Val 610 cca Pr o tgg Tr p aac Asn gcc Al a at t SIle 690 gt c Val gcc Al a ttc Phe cag Sn gga G y 770 Phe 595 ct g Leu gct Al a cag G n gcg Al a gct Al a 675 cag G n ggc G y gga G y ttc Phe gcc Al a 755 ggg G y ggt G y gtt Val gtt Val aag Lys aac Asn 660 aaa Lys ggc G y gca Al a aca Thr gcc Al a 740 at g Met cac Hi s at c SIe gtt Val cct Pr o at t SIe 645 ttc Phe t ct Ser at c SIe gcc Al a cct Pr o 725 cca Pr o tgg Tr p 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2416 2439 t aaaacat ga tggctct t ac t ca <210> 14 <211> 772 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 14 Val Ala Leu Lys Arg Pro G u u Lys Thr Val Lys lie Val Thr Ile 1 5 10 Lys G n Thr Asp Asn I e Asn Asp Asp Asp Leu Val Tyr Ser Asn Ala 25 Thr Asp Leu Pro Val G y Val Lys Lys Ser Pro Lys IVet Ser Pro Thr 40 Ala Arg Val y Leu Leu Val Phe y Val I e Ala Ala Val y Trp Page 21 seqi i st car r ect ed. t xt Asn Ser Val Trp G31y ValI Ar g Pr o Ar g Le u 145 Le u Le u vb t A a ValI 225 vb t Ar g Le u ValI G31y 305 Pr o Le u ValI Le u Ph e 385 I Ie A a I Ie O31u 115 Le u Gy le Le u Ar g 1 95 li1e As n I Ie G31y A a 275 Tr p G31y As p ValI Ser 355 Pr o A a Ph e ValI Tyr ValI G31y ValI Le u 1 65 ValI Ou le Le u I Ie 245 ValI ValI Thr vb t Le u 325 li1e A a Le u I Ie Ar g 405 Ser 70 (31y Ly s As n Hi s vb t 150 G31y Ser vb t vb t A a 230 A a Thr A a Tr p A a 310 Ser Le u Le u Ph e Ser 390 Ar g 31y O31u Th r Ser ValI As p Hi s 135 A a ValI Thr G31y I Ie 215 As p Le u O31u G31y Ser 295 A a Thr Ph e O31u I Ie 375 Ser I Ie Ly s 1 05 Ly s A a O31n Ph e Ar g 1 85 ValI I Ie Pr o vb t Ser 265 Tr p Thr Le u vb t Ar g 345 As n I Ie Thr lIe 90 Pr a As p A a vb t A a 1 70 Ar g Gy le Tr p G31y 250 li1e ValI Thr Pr a Ly s 330 Pr a G31y A a Thr lIe 75 A a Ly s Tyr G31y 1 55 G31y G31y G31y A a G31y 235 ValI I Ie A a Le u ValI 315 li1e O31u Pr a cys Pr a 395 Ph e As p ValI A a 140 Tyr A a Ar g A a ValI 220 ValI Tyr G31y As p A a 300 Tr p G31y ValI ValI G31y 380 Ly s Ser O31n Pr a 125 G31y Le u ValI Ser A a 205 Le u Ph e Le u ValI Thr 285 Le u Le u ValI O31n Ph e 365 A a Le u Ph e Ar g 110 Thr A a Pr a O31n Le u 1 O31y A a Ser Ar g A a 270 Ser A a Le u I Ie vb t 350 Ser Le u ValI Tyr A a As p O31y O31y As p 1 Oy le Le u I Ie Tyr 255 Le u Tr p Le u Le u O31y 335 Pr a O31y Ser O31u Le u A a Thr Ar g Pr a Thr 1 Tyr O31n Le u I Ie Thr 240 Le u Le u Oy le A a 320 Le u Ser Ser O31y Ly s 400 03 u Ser 03 n [Vbt l'b t L eu O31y Ty r 03 O31y l'bt Leu l'bt O31u Ser 415 Page 22 seqi i st car r ect ed. t xt Phe Val Ala l'bt l'bt Ala Leu I Ie Thr Ala Val I Ie Leu Asp Ar g Hi s 430 420 Le u A a I Ie 465 Thr Ser Tr p ValI G31y 545 G31y Le u Pr o Le u I Ie 625 Ser O31n G31y vb t ValI 705 A a O31u ValI Ph e 435 A a Pr o I Ie I Ie Hi s 515 A a ValI Tr p vb t Ph e 595 Le u A a O31n A a A a 675 O31n G31y G31y Ph e A a 755 vb t (31u O31n Ar g 485 Ser A a Thr O31y Ser 565 ValI I Ie ValI Pr o I Ie 645 Ph e Ser I Ie A a Pr o 725 Pr a Tr p A a ValI 455 Ser O31y Ph e vb t ValI 535 Ar g ValI As p As n ValI 615 A a Hi s As p O31u Ser 695 A a O31u O31y (31u Pr a 440 As n O31u O31y I Ie Ph e 520 A a Ar g Ph e Pr a O31n 600 Lys5 Tr p Ser A a A a 680 li1e ValI Thr Ph e Ar g 760 425 Le u Ser A a A a O31y 505 O31u Ar g Ph e ValI Le u 585 Le u Lys5 As p As p Lys5 665 li1e Le u cys Thr Le u 745 Tyr A a I Ie A a Pr a 490 O31y A a Ph e Lys5 cys 570 O31y Le u O31y Le u Pr a 650 Ser As p Ph e I Ie O31u 730 A a Pr a Le u O31y O31u 475 Thr A a Le u vb t As p 555 A a O31y A a Le u I Ie 635 A a O31n A a A a Lys5 715 O31u Ser (31y O31y 445 Thr ValI A a vb t I Ie 525 Thr Ser Tr p As n I Ie 605 Ly s Thr O31y Le u I Ie 685 Le u I Ie As p Ar g A a 765 As p A a O31u O31y 495 A a Thr Thr Thr A a 575 Le u Le u A a Thr Tr p 655 O31u As n Le u A a O31u 735 Ly s ValI Ser 0 y 0 y Hi s 770 Page 23 seqI i st car r ect ed. t xt <210O> <211 <21 2> <21 3> <220> <221 <222> <223> 255 DNA Cor ynebact er i urn gi ut amr cumn CIDS 101). (232) RXN031 19 <400> t gggaggt gt cgcaccaagt actt t tgcga agcgccat ct at atgct cgg t gcggaaacc t acgaaagga tt tt t taccc gacggat t tt caaaagat gt at g gct gt a t ac gaa 11! [Vbt Ala Val Tyr Gu ct C cca gaa ct C Leu Pr o G31u Leu gac tac gca tac gac gct ctc gag cca cac atc gtc As p Ty r Al a Ty r As p Al a L eu G31u Pr o Hi s I Ie ValI 15 gct gaa at c Al a G3 u I I e gag ct t gac G3 u Leu Asp cag t cc aag G3 n Ser Lys gac cac gca As p Hi s Al a acc t ac gt t Thr Tyr Val gcg ggc gca aat gca gca ctc taggcactag agaaggcacg cga Al a Gy Al aAsn Al aAl a Leu <210O> <211 <21 2> <21 3> <400> Nib t Al 1 16 44 PmT Cor ynebact er i urn gI ut anm curn 16 a Val Tyr a31u Leu Pro a31u Leu Asp 5 10 Tyr Al aTyr Asp Al a Leu au Pro His IleVal Alaa3u Ile I'bt 25 His Ala Thr Tyr Val Ala ay Ala Asn <210O> 17 <21 1> 249 <212> DNA <21 3> Cor ynebact er i urn gl ut anm curn a3 u Leu Asp a3 n Ser Lys Asp Al a Al a Leu <220> <221 <222> <223> OIDS 101). (226) RXN031 <400> 17 ct gggcagt t ct t gggt acg accacat at c cggt cgcct g gt t at cgagc agct caccga ccaggagggc aacat ct cct t cgacat cac cccagt t ct g gag cac gct ttc tac ctg cag tac atg aac gtt (3u Hi s Al a Phe Tyr Leu (31n Tyr N/t Asn Val 15 Page 24 at g ct c gat at g t gg I'b t Leu Asp I'bt Tr p 1 aag gca gat t ac gt C Lys Al a Asp Tyr Val seql i st cor r ect ed. t xt aag gct gtt tgg aac gtc ttc aac tgg gac gac gca aga gca cgc ttc Lys Ala Val Trp Asn Val Phe Asn Trp Asp Asp Ala Arg Ala Arg Phe 30 gca gca gct tcc aag taagcatttt tagtccgtgc aat Ala Ala Ala Ser Lys <210> 18 <211> 42 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 18 lIet Leu Asp lIet Trp G u His Ala Phe Tyr Leu G n Tyr lIet Asn Val 1 5 10 Lys Ala Asp Tyr Val Lys Ala Val Trp Asn Val Phe Asn Trp Asp Asp 25 Ala Arg Ala Arg Phe Ala Ala Ala Ser Lys <210> 19 <211> 660 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(637) <223> RXN00575 <400> 19 gaagcgtccg caggcaagaa aacgtcccgg aaaacggcga ccggttgccg atctatttaa ataccaggac aattgcgtgc taggggtcac ccgcgcatgt atg gtt gaa lMt Val Gl u ttc Phe at c SIle ggt Gy at g Met tct Ser gat Asp at c SIle gaa G u acg Thr gcg Al a gct Al a at c SIle cca Pr o ggc Gy gac Asp gaa G u at t l Ie at c SIle cgc Ar g gag G u ttc Phe aac Asn agc Ser ccc Pr o ggt G y gga Gy caa G n ccg Pr o cag G n ggc Gy gat Asp 15 cat Hi s tct Ser gac Asp gtt Val caa Sn 95 ttc Phe gcg Al a aaa Lys ttc Phe ttc Phe 80 ggc Gy 1 cct Pr o act Thr at c SIle at c SIle cat Hi s cga Ar g cag G n t at Tyr at g Mat ct c Leu gga gay gga Gy aga gac Arg Asp agg gac Arg Asp act tct Thr Ser acc gtc Thr Val tgg gtg Trp Val gtg gaa Val G u ggc ggc 1y 00 100 gct ttg ctc gac gcc ctt atc acc tac tgc gaa agc aac ggc atc tgg Page seqI i st car r ect ed. t xt Al a Leu Leu Asp Al a Leu I I e Thr Tyr Cy s (3 u Se r Asn 105 110 t cg at c cac t cc t gg at c t tc ccg gaa aac ct c ggt t ct Ser Ilie Hi s Ser Tr p Ilie Phe Pr o O31u Asn Leu O31y Ser 120 125 130 cat gaa t cg aag ggc t tc gt g aag gt g ggc acc at g cac His O31u Ser Lys O31y Phe Val Lys Val O31y Thr I'b t Hi s 135 140 145 agg at g ccc t ac ggc gag at g gaa gga caa t gg cgc gat Ar g I'b t Pr o Tyr O31y O31u b t O31u O31y O31n Tr p Ar g Asp 150 155 160 t gg gag tgc ctc tt a tcc gtt cca gag caa gct caa agt Trp O31u Cys Leu Leu Ser Val Pr o 31u O31n Al a O31n Ser 170 175 t aaagcaat t t aaat ct gac tt t <210> <211> 179 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn O31y 115 gcg Al a c aa O31n t gt Cys t cc Ser I Ie Tr p aaa ct g Lys Leu at g gca [vb t Al a gat ct g Asp Leu 1 <400> [vb t ValI Pro O31n Thr Tyr I e I'b t 1 1 e Leu Hi s 03 y Ar g O31y Ser As n O3 y Ser 130 I'b t Hi s 145 Ar g As p O3 n Ser (31u Ar g Asp Val Ar g Asp (3 u Thr Ser As p Thr Val (31y Tr p Val Val Val O31u li1e O31y O31y 1 00 (31y I Ie Tr p 115 Al a Lys Leu (3 n [Vb t Al a Cys Asp Leu 1 65 Ser Phe Thr I Ie Tyr Pr a ValI Al a Ser Le u I Ie O31u 135 vb t O31u O31u Thr 40 Al a Al a I Ie Le u Hi s 120 Ser Pr a Cys Ar g Le u Tr p ValI Pr a Tyr As p 1 Ser Lys5 Tyr Le u I Ie Le u O31n As n Ser 75 Hi s Le u I Ie Ph e O31u 1 55 Ser O31u Thr Ser As p Ar g O31n Thr Pr a 125 Lys5 O31u Pr a As p Hi s Ser As p ValI O31n Cys As n O31y O31n O31n 1 Ph e Al a Ph e Ph e O31y O31u Le u Thr Tr p 1 Al a <210O> 21 <21 1> 606 <212> DNA Page 26 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(583) <223> FRXA00575 <400> 21 gcatgtccgg ttgccgatct atttaaatac caggacaatt cttcactatc cgaccaatcc gcgagggtga tttccctcag gcgtgcatgg ttgaaagaga gtg agg gac atc tac 11! Val Arg Asp Ile Tyr gaa ttg ggc ctg G u Leu G y Leu gag Su acg gga cat gcg Thr G y Hi s Al a tat gag act tct Tyr G u Thr Ser ggt ccc G y Pro acg tgg gac Thr Trp Asp gcg gta gaa Ala Val u ttc tcc caa tct Phe Ser G n Ser aaa Lys atc atg gat acc I e IVet Asp Thr gt c at g gt g Val Met Val gtg tct gct Val Ser Al a aac aac gac ccg Asn Asn Asp Pro ttc atc ctc gga Phe Ile Leu a y gct cca Ala Pro att tca agc cga lie Ser Ser Arg gtt ttc cat gga Val Phe His G y gtg gaa gat tcc Val G u Asp Ser atc tat atc cac ccc cag ggc caa ggc cga gga atc ggc ggc gct ttg IIe Tyr lie His Pro G n Gy n Gy Arg Gy lie Gy Gy Ala Leu 75 80 ctc gac gcc ctt Leu Asp Ala Leu at c I e acc tac tgc gaa Thr Tyr Cys G u aac ggc atc tgg Asn y lie Trp tcg atc Ser Ile 100 cac tcc tgg His Ser Trp tcg aag ggc Ser Lys G y 120 ttc ccg gaa aac Phe Pro G u Asn ct c Leu 110 ggt tct gcg aaa G y Ser Ala Lys ctg cat gaa Leu His G u 115 gca agg atg Ala Arg IVet ttc gtg aag gtg Phe Val Lys Val acc atg cac caa Thr Met His G n at g Met 130 ccc tac Pro Tyr 135 ggc gag atg gaa G y G u Met G u caa tgg cgc gat Sn Trp Arg Asp gat ctg tgg gag Asp Leu Trp G u t aaagcaat t tgc Cys 150 ctc tta tcc gtt Leu Leu Ser Val cca Pr o 155 gag caa gct caa Su G n Ala n agt t cc Ser Ser 160 taaatctgac ttt <210> 22 <211> 161 <212> PRT <213> Corynebacter i um gl utam cum <400> 22 Val Arg Asp Ile Tyr u Leu y Leu u 1 5 10 Thr G y His Ala Thr G u Thr Ser G y Pro Thr Trp Asp G n 25 Phe Ser G n Ser Lys II e \et Page 27 Asp Sy Val I le Sy Al a G n Cys 145 Ser Val Val G u Gy Tr p Leu 115 Al a Leu Val Al a Ser Leu I le G u t u Gu Al a Al a I e 70 Leu Hi s Ser Pr o Cys 150 Val Pr o Tyr Asp Ser Lys Tyr 135 Leu seql i st cor r ect ed. t xt Su Asn Asn Asp Pro Asp 40 lie Ser Ser Arg G n Val lie His Pro n Gy n Ala Leu I e Thr Tyr Cys 90 Trp lie Phe Pro G u Asn 105 Sy Phe Val Lys Val y 120 125 Gy Gu Met Gu Gy n 140 Leu Ser Val Pro G u G n 155 SIe Hi s Ar g Ser Sy Met Ar g G n <210> 23 <211> 1575 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1552) <223> RXN01345 <400> 23 cataacctca ttgaacatgc aaaactaatg cttttggggg ttccactgca cagcccgaaa atgctgctag ggtcaagttc gac Asp t at Tyr at t SIle gat Asp aaa Lys ttg Leu gga Sy at c SIle tct Ser at t SIle ct a Leu gat Asp acc Thr act Thr gt g Val gtt Val tct Ser gt a Val cgc Ar g gt g Val gcc Al a ggg Sy gaa G u 75 cac Hi s aca Thr gaa G u ct c Leu cag G n ccc Pr o cct Pr o gcg Al a tct Ser gca Al a cac Hi s gt c Val ggc G y gcc gcc Al a Al a 15 tta ggc Leu G y gat agg Asp Arg cct t cc Pro Ser acg gaa Thr G u 80 gcc gtc Al a Val Page 28 gtatgcataa at t cgtttcg atg cgt ttt gga ctt IVet Arg Phe y Leu 1 gtg gac cgc gga aac Val Asp Arg y Asn gac acc cac gat ttc Asp Thr His Asp Phe att gtc gcg ggt tgg Ile Val Ala y Trp ttc gta cgt tct ttc Phe Val Arg Ser Phe gcc acc ccg gtc tac Ala Thr Pro Val Tyr ctg gag gct ttt gcg Leu G u Ala Phe Ala seqI i st car r ect ed. t xt g aa O31u ac c Thr gcc A a gt t ValI 1 50 c ac Hi s t at Tyr G31y G31y G31y 230 g ac As p tt g Le u tt c Ph e g aa O31u at c I Ie 310 ct c Le u ggt G31y gt g ValI ccg Pr o 120 cga Ar g ggt G31y c ac Hi s tt g Le u c ac Hi s 200 g at As p c ag O31n t cc Ser ct a Le u gag O31u 280 ct c Le u ct t Le u gag O31u act Thr act Thr g aa O31u ct c Le u gt c ValI cgc Ar g 1 70 ggc G31y gag O31u g at As p g at As p aac As n 250 at t I Ie gcc A a 99c G31y ggt G31y Ph e 330 gt g ValI gcg A a gt a ValI acc Thr aat As n 155 acc Thr gga G31y gt t ValI gaa O31u gcg A a 235 gcg A a 99c G31y act Thr gt c ValI gga G31y 315 99c G31y ggt G31y ct g Le u gt c ValI at g vb t 140 gag O31u tt a Le u ac a Thr gt g ValI at c I Ie 220 tt t Ph e aag Ly s g ac As p ccc Pr o g at As p 300 gga G31y cgc Ar g ct g Le u cgt Ar g at t I Ie 125 t cc Ser ccc Pr o aac As n tt c Ph e t cc Ser 205 ct c Le u ggc G31y g aa O31u g ac As p ct g Le u 285 g at As p t cc Ser cgt Ar g gcc A a gca A a 110 ggt G31y gcc A a ag c Ser t cc Ser g ac As p 1 t cc Ser ct c Le u aag Ly s gct A a g ac As p 270 gt g ValI ct t Le u t cg Ser gt c ValI at c I Ie 350 tt t Ph e gt g ValI Ph e gcc A a aag Ly s 1 75 t cc Ser at t I Ie c aa O31n cgt Ar g ct t Le u 255 at c I Ie g aa O31u aaa Ly s ct c Le u c ac Hi s 335 gcg A a c ag O31n ccc Pr o ag c Ser gca A a 1 60 cgc Ar g t cg Ser ggc G31y t gc cys gct A a 240 gt t ValI ac c Thr aaa Ly s g at As p cca Pr a 320 cgc Ar g gct A a ac g Thr gcc A a gcc A a 145 gct A a c aa O31n ct c Le u at t I Ie gcg A a 225 aaa Ly s ccg Pr a gt t ValI t cc Ser t cc Ser 305 ct c Le u t cc Ser g ac As p c aa O31n aac As n 130 ac a Thr tt c Ph e gcc A a at c I Ie t ca Ser 210 ct c Le u aac As n c aa O31n cca Pr a tt g Le u 290 g ac As p gt t ValI cca Pr a cct Pr a gga O31y c ac Hi s at c I Ie t ac Tyr gt g ValI 1 at c I Ie ct t Le u gcc A a ct t Le u cgt Ar g 260 aac As n at c I Ie gca A a ag g Ar g ccc Pr a 340 t ct Ser g at As p ag c Ser ac c Thr ac c Thr 1 gt t ValI g ac As p ggt O31y gca A a ct c Le u 245 cgc Ar g aag Ly s at g O31y tt g Le u 325 t ca Ser ggt (31y 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 ttc cac cta agg gac cgc gtt gcg cga ggc Phe His Leu Arg Asp Arg Val Ala Arg Oy atc ggt gtg ttc cgt gag Ilie Oy Val Phe Arg Ou Page 29 seql i st cor r ect ed. t xt 360 365 370 cac gat tct ggt cgt gcc gtg agc ttt gac ccg ctg atc His Asp Ser G y Arg Al a Val Ser Phe Asp Pro Leu I I e 375 380 385 acc gat tct gcg acc gtg gcg aaa cga tgc tac aag gcg Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr Lys Ala 390 395 400 att ggt tgg ttc agg ttc gtg gaa tac tcc acc gtg tcc lie y Trp Phe Arg Phe Val G u Tyr Ser Thr Val Ser 410 415 agc ccc gga gat att tcc ctg ctc agt gaa atc aag at t Ser Pro y Asp Ile Ser Leu Leu Ser Gu Ile Lys Ile 425 430 agc tcc atc acc gat gtg gat gct acc gag att tca cgt Ser Ser lie Thr Asp Val Asp Ala Thr G u lie Ser Arg 440 445 450 cca gaa gta gaa gaa acc atc aca gtc aat gac aac ggc Pro u Val Gu u Thr lie Thr Val Asn Asp Asn y 455 460 465 att tcc atc aag ata ctc ggc ggc gtt acc gtc gag cac IIe Ser IIe Lys IIe Leu G y G y Val Thr Val G u Hi s 470 475 480 tagttaccat tttggtgctg gtg <210> 24 <211> 484 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 24 gcc Al a gt g Val gag G u cct Pr o 435 ttc Phe gt g Val aca Thr ccg Pr o cac Hi s gat Asp 420 ttt Phe gat Asp gct Al a at t SIe 1267 1315 1363 1411 1459 1507 1552 1575 Met 1 Val Asp SIle Phe Al a Leu Thr Al a Al a 145 Arg Phe y Leu Asp Leu y Thr Ar g Hi s Al a Ar g Pr o Al a Leu 115 Ser Gy 5 Asn Phe Tr p Phe Tyr Al a Asp Ser Thr Tyr SIle Asp Lys 70 Leu G u Thr Al a Val 150 Pr o Pr o Al a Ar g Gy Asn Ser G n 135 Val Val Val G u Leu Hi s Val 105 I le Leu Leu Thr Arg 10 Thr Val Val Ala Val G y Ser G u Val Hi s 90 Thr Al a G u Val Leu Thr Val Asn 155 Page SIle Asp Lys Asp Asn Leu Ar g SIle 125 Ser Pr o Al a Ser Al a Hi s Val Gy Al a 110 Gy Al a Ser Al a Leu Asp Pr o Thr Al a Phe Val Phe Al a seqi i st car r ect ed. t xt A a Phe 03 u Tyr Thr 1 65 His Arg His Ala Ar Thr Leu Asn Ser Lys Ar g 1 O31n Le u I Ie A a 225 Ly s Pr o ValI Ser Ser 305 Le u Ser As p G31y Le u 385 Ly s ValI Ser As n 465 (31u I Ie Ar g 1 95 Ar g Lys5 Th r Ser ValI 275 Ser I Ie Ser Ph e Ser 355 Ph e A a ValI G31u Pr o 435 Ph e ValI Th r ValI As p G31y A a Le u 245 Ar g Lys5 vb t G31y Le u 325 Ser G31y G31u As p As n 405 G31y As p G31y Ser As p Th r As p 215 Ar g G31u ValI Ty r Pr o 295 Ty r Ar g Ser Hi s As p 375 As p G31y Pr o Ser G31u 455 Ser G31y 1 Hi s Ph e Hi s Ar g G31u 265 A a I Ie ValI Ar g A a 345 Ar g G31y A a Ph e As p 425 Th r G31u Lys5 G31y ValI G31u A a 235 A a G31y Th r ValI G31y 315 G31y G31y Ar g A a ValI 395 Ph e Ser ValI Th r Le u 475 Ph e Ser 205 Le u G31y G31u As p Le u 285 As p Ser Ar g A a A a 365 Ser Lys5 G31u Le u A a 445 Th r G31y Ser I Ie O31n Ar g Le u 255 li1e O31u Ly s Le u Hi s 335 A a G31y As p cys Ser 415 O31u O31u As n Th r Ser G31y cys A a 240 ValI Th r Lys As p Pr a 320 Ar g A a I Ie Pr a Ty r 400 Th r I Ie I Ie As p ValI 480 <210O> <21 1> 1261 <212> DNA Page 31 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1267) <223> FRXA01345 <400> cataacctca ttgaacatgc aaaactaatg cttttggggg ttccactgca cagcccgaaa atgctgctag ggtcaagttc gtatgcataa attcgtttcg atg cgt ttt gga ctt 11! IVet Arg Phe Gy Leu 1 gac ttg gga act Asp Leu G y Thr acc Thr cgc aca atc gcg Arg Thr Ile Ala gcc Al a 15 gcc gtg gac cgc Ala Val Asp Arg gga aac G y Asn tat ccc atc Tyr Pro Ile at t cca t ct Ile Pro Ser act gtg gaa gat Thr Val G u Asp tct Ser tta ggc gac acc Leu G y Asp Thr cac gat ttc His Asp Phe gcg ggt tgg Ala G y Trp gtg gtg gcc ctc Val Val Al a Leu gca gat agg att Ala Asp Arg lle gt c Val gat gct Asp Al a att gag gtt lie G u Val ggg cag G y G n gac cac cct t cc Asp His Pro Ser gta cgt tct ttc Val Arg Ser Phe aaa Lys cgc cta ctc tct Arg Leu Leu Ser gaa Gu 75 ccc aat gtc acg Pro Asn Val Thr gaa Gu gcc acc ccg gtc Ala Thr Pro Val ttg ggc gat cat Leu G y Asp Hi s gt a Val cac cct ttg ggc His Pro Leu G y gcc Al a 95 gtc ctg gag gct Val Leu G u Ala ttt gcg Phe Al a 100 gaa aac gt g G u Asn Val acc tcc ccg Thr Ser Pro 120 act gcg ctg cgt Thr Ala Leu Arg gca Al a 110 ttt cag acg caa Phe G n Thr G n ttg gga gat Leu G y Asp 115 atc gaa gta gtc lie u Val Val att ggt gtg ccc gcc aac tcc cac agc Ile y Val Pro Ala Asn Ser His Ser 125 130 gcc cag Al a G n 135 cga ctg ctc acc Arg Leu Leu Thr at g Met 140 tcc gcc ttc agc Ser Ala Phe Ser gcc Al a 145 aca ggc atc acc Thr G y Ile Thr gtt Val 150 gtc ggt ttg gtc Val G y Leu Val gag ccc agc gcc G u Pro Ser Ala gct ttc gag tac Ala Phe G u Tyr cac cgc cac gcc His Arg His Ala cgc Ar g 170 acc tta aac tcc Thr Leu Asn Ser cgc caa gcc atc Arg G n Ala lle gtg gtt Val Val 180 tat gat ttg Tyr Asp Leu ggc acc cac G y Thr Hi s 200 ggc gga aca ttc Sy y Thr Phe gac Asp 190 t cc tcg ctc at c Ser Ser Leu Ile cgc atc gac Arg lie Asp 195 cgc ctt ggt Arg Leu G y cac gag gtt gtg His G u Val Val tcc att ggc att Ser lie G y lle t ca Ser 210 ggc gat gat ttc gat gaa atc ctc ctc G y Asp Asp Phe Asp G u Ile Leu Leu caa tgc gcg ctc aag gcc gca G n Cys Ala Leu Lys Ala Ala Page 32 seql i st cor r ect ed. t xt 220 ggc aga cag cac gat gcg ttt G y Arg G n His Asp Al a Phe 230 235 gac gaa tcc cgc aac gcg aag Asp u Ser Arg Asn Al a Lys 250 ttg gtt cta gaa att ggc gac Leu Val Leu G u lie G y Asp 265 ttc tac gag gct gcc act ccc Phe Tyr G u Ala Ala Thr Pro 280 gaa ccc ctc atc ggc gtc gat Su Pro Leu lie y Val Asp 295 300 atc tac ctt gtt ggt gga gga Ile Tyr Leu Val Gy Gy y 310 315 ctc cgc gag cgt ttc ggc cgc Leu Arg G u Arg Phe G y Arg 330 ggt tcc act gcg gtg ggt ctg Sy Ser Thr Ala Val G y Leu 345 ttc cac cta agg gac cgc gtt Phe His Leu Arg Asp Arg Val 360 cac gat tct ggt cgt gcc gtg His Asp Ser G y Arg Al a Val 375 380 <210> 26 <211> 389 <212> PRT <213> Corynebacter i um gl ut a <400> 26 IVet Arg Phe y Leu Asp Leu 1 5 Val Asp Arg Gy Asn Tyr Pro Asp Thr His Asp Phe Ile Pro Ile Val Ala Gy Trp Asp Al a Phe Val Arg Ser Phe Lys Arg 70 Ala Thr Pro Val Tyr Leu y Leu G u Ala Phe Ala G u Asn ggc Gy gaa G u gac Asp ct g Leu 285 gat Asp t cc Ser cgt Ar g gcc Al a gcg Al a 365 aag Lys gct Al a gac Asp 270 gt g Val ctt Leu tcg Ser gt c Val at c l Ie 350 cga Ar g cgt Ar g ctt Leu 255 at c SIle gaa G u aaa Lys ct c Leu cac Hi s 335 gcg Al a ggc Gy gct Al a 240 gtt Val acc Thr aaa Lys gat Asp cca Pr o 320 cgc Ar g gct Al a at c SIle 225 aaa Lys ccg Pr o gtt Val t cc Ser t cc Ser 305 ct c Leu t cc Ser gac Asp ggt Gy aac Asn caa G n cca Pr o ttg Leu 290 gac Asp gtt Val cca Pr o cct Pr o gt g Val 370 ctt Leu cgt Ar g 260 aac Asn at c SIe gca Al a agg Ar g ccc Pr o 340 tct Ser cgt Ar g 835 883 931 979 1027 1075 1123 1171 1219 1267 agc ttt gac ccg ctg atc gcc ccg gac Ser Phe Asp Pro Leu lie Ala Pro Asp ri cum G y Thr Ile Val 25 Ser Val 40 lie G u Leu Leu Asp Hi s Val Val Thr Arg 10 Thr Val Val Ala Val G y Ser G u Val Hi s 90 Thr Al a Page 33 SIle Asp Lys Asp Asn Leu Ar g Al a Ser Al a Hi s Val Gy Al a Al a Leu Asp Pr o Thr Al a Phe seql i st cor r ect ed. t xt 105 Il e G u Val Thr G n Leu 115 Al a Asn Ser 130 Al a Thr G y 145 Al a Phe G u G n Ala I e Leu II e Ar g 195 I e Ser Ar g 210 Ala Leu Lys 225 Lys Asn Thr Pro G n Ser Val Pro Val 275 Ser Leu Ser 290 Ser Asp Ile 305 Leu Val Ser Ser Pro Phe Asp Pro Ser 355 G y Val Phe 370 Leu Ile Al a 385 Asp Ser Thr Thr 165 Val Asp Sy Al a Leu 245 Ar g Lys Met Sy Leu 325 Ser Sy G u Asp Thr Ser Pro 120 Al a G n Ar g 135 Val Val G y 150 His Arg Hi s Tyr Asp Leu Sy Thr His 200 G y Asp Asp 215 G y Arg G n 230 Asp G u Ser Leu Val Leu Phe Tyr G u 280 G u Pro Leu 295 Ile Tyr Leu 310 Leu Arg u G y Ser Thr Phe His Leu 360 Hi s Asp Ser 375 Leu Leu Al a Sy 185 Hi s Phe Hi s Ar g G u 265 Al a SIle Val Ar g Al a 345 Ar g Sy Thr Asn 155 Thr Sy Val G u Al a 235 Al a Sy Thr Val Sy 315 Sy Sy Ar g Al a Val lile 125 Mbt Ser 140 G u Pro Leu Asn Thr Phe Val Ser 205 II e Leu 220 Phe G y Lys G u Asp Asp Pro Leu 285 Asp Asp 300 G y Ser Arg Arg Leu Al a Val Ala 365 Val Ser 380 Sy Al a Ser Ser Asp 190 Ser Leu Lys Al a Asp 270 Val Leu Ser Val l Ie 350 Ar g Phe Val Phe Al a Lys 175 Ser SIle G n Ar g Leu 255 I le G u Lys Leu Hi s 335 Al a Sy Asp Pr o Ser Al a 160 Ar g Ser Sy Cys Al a 240 Val Thr Lys Asp Pr o 320 Ar g Al a SIe Pr o <210> 27 <211> 1308 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1285) <223> RXA02541 <400> 27 Page 34 seql i st cor r ect ed. t xt atccgccggt gtccggacaa caaaacttgc aacacaagat aacttaagaa attgcataca attcaccgca tataagactc atggaaggag gggatgccca gtg Val 1 aac aac agc Asn Asn Ser gaa Gu tgg gca aat aag Trp Ala Asn Lys aac Asn tat tac gca gac Tyr Tyr Ala Asp ggg gtc tcc tcg G y Val Ser Ser t cc gct Ser Ala tca gaa gat Ser G u Asp cac cca gat His Pro Asp atc aaa aag gct Ile Lys Lys Al a cgc aag ctc gcc Arg Lys Leu Ala agg gaa aat Arg G u Asn cga ttc aaa Arg Phe Lys aaa aat cca ggt Lys Asn Pro G y aag gcc gct gaa Lys Ala Ala G u aaa gcg Lys Al a gcc gag gca tat Ala G u Ala Tyr gac Asp gta ctt ggt gat Val Leu y Asp aag aaa cga aaa Lys Lys Arg Lys gaa Su tac gac gag ct c Tyr Asp G u Leu aaa Lys gca ctt cta gct Ala Leu Leu Al a ggt gga atc cgc Sy y lie Arg gga ttc gga agc G y Phe G y Ser gga Sy ggt gcg gga ttc G y Al a G y Phe ggc ggg ttt cgc G y G y Phe Ar g acg tcg Thr Ser 100 acg gga gga Thr G y G y ggg ttt tct G y Phe Ser 120 gac acc tca gac Asp Thr Ser Asp ct c Leu 110 ttc gga gga gga Phe G y G y G y caa ggt gga G n y G y 115 ggc ctt ttc G y Leu Phe acg gac ggc ggt Thr Asp G y G y ggc gat atc ttc G y Asp Ile Phe ggt 30y 130 aac cgc Asn Arg 135 ggc gct ggt tct G y Al a G y Ser cac Hi s 140 cag tca gct agg G n Ser Ala Arg ccg acg cgg ggg gcg Pro Thr Arg y Ala 145 gag gca gcg aaa ggc G u Al a Al a Lys G y gat Asp 150 gta caa acc gaa Val G n Thr G u at a Ile 155 act ctc tcg ttt Thr Leu Ser Phe gtt Val 160 acg acc atc cca Thr Thr Ile Pro gtg Val 170 gaa ctc acc ggc G u Leu Thr G y gcg ccc tgc aac Ala Pro Cys Asn acc tgc Thr Cys 180 cac gga t cg Hi s G y Ser t cc aaa tca ggc Ser Lys Ser G y cac Hi s 190 ccc gca aaa tgt Pro Ala Lys Cys gga acc tgt G y Thr Cys 195 gga ttc tcc G y Phe Ser gat gga acc gga ttc acc tct Asp G y Thr G y Phe Thr Ser 200 aac aag ggt gct Asn Lys G y Al a ttc Phe 210 gct cca Ala Pro 215 tgt gca acc tgt Cys Al a Thr Cys ggt Sy 220 ggc act ggt gaa G y Thr G y G u at a Ile 225 at c act gat cct Ile Thr Asp Pro tgc gat aac tgc cac ggc cga ggc acc gtc cgg Cys Asp Asn Cys His G y Arg G y Thr Val Arg 230 235 240 aag tct cgt tcc Lys Ser Arg Ser acc gtg cgt atc cca act ggt gtg gaa Thr Val Arg Ile Pro Thr y Val G u gat gga cag aaa gtt cgt ctt Asp y G n Lys Val Arg Leu Page seql i st cor r ect ed. t xt 250 gca ggc caa ggc gaa gca gga cca aat Ala G y n G y G u Ala G y Pro Asn 265 270 ttt gtg aaa gtc cac gtg aaa aag gac Phe Val Lys Val His Val Lys Lys Asp 280 285 agc aac att ttg atc acc att ccc gtg Ser Asn Ile Leu Ile Thr Ile Pro Val 295 300 ggt ggc gct att tct gtg cca acg ctc G y G y Ala IIe Ser Val Pro Thr Leu 310 315 cta cct gcg gga acg cca gat ggt cgt Leu Pro Ala y Thr Pro Asp y Arg 330 ggt atc gaa gca cgt gat tcc act ggt Gy lie Gu Ala Arg Asp Ser Thr y 345 350 gtt tct gtc ccg aag aat ctg gat gac Val Ser Val Pro Lys Asn Leu Asp Asp 360 365 gca tat gct gaa gca gaa act aat t ca Ala Tyr Ala G u Ala G u Thr Asn Ser 375 380 tgg gcg ggc cag aac cgc tagacgttct Trp Ala G y G n Asn Arg 390 395 <210> 28 <211> 395 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 28 Val Asn Asn Ser G u Trp Ala Asn Lys 1 5 Val Ser Ser Ser Ala Ser G u Asp G u Leu Ala Arg G u Asn His Pro Asp Lys 40 G u Asp Arg Phe Lys Lys Ala Ala u 55 Asp Lys Lys Arg Lys G u Tyr Asp G u 70 Gy Gy Ile Arg Gy y Phe y Ser Gy Phe Arg Thr Ser Thr Gy Gy Phe 100 105 G y G y n G y G y G y Phe Ser Thr 115 120 aaa Lys gt g Val ttc Phe aag Lys 320 ttg Leu ct g Leu gct Al a ttt Phe cca Pr o ttc Phe agc Ser 305 cct Pr o cgt Ar g ct g Leu gcg Al a gat Asp 385 gcg Al a aca Thr 290 gag G u gt a Val gt a Val gtt Val gaa G u 370 ccc Pr o ggc Gy 275 cgc Ar g ct g Leu aaa Lys cgc Ar g aca Thr 355 gct Al a cgc Ar g 260 gat Asp gac Asp gct Al a ct c Leu ggt Gy 340 gt c Val ct c Leu gct Al a 931 979 1027 1075 1123 1171 1219 1267 1308 ctttgagaaa gga Asn Tyr Tyr 10 I e Lys Lys Asn Pro y Al a Tyr Asp Leu Lys Al a G y G y Al a 90 Asp Thr Ser Asp G y y Page 36 Al a Al a Asp Val Leu Gy Asp Leu 125 Leu Ar g Al a Gy Al a Pr o Phe Asp seqi i st car r ect ed. t xt Ph e (31y 31y Le u Ph e As n Ar g 130 135 G3 y A a G3 y Se r His On Ser Ala Arg Pr o 145 (31u Pr o Ly s A a I Ie 225 Ly s (31n Pr o Ph e Ser 305 Pr o Ar g Le u A a As p 385 Ar g A a As n G31y 1 95 G31y Thr Ar g ValI G31y 275 Ar g Le u Lys5 Ar g Thr 355 A a Ar g A a G31y 1 65 cys cys Ser Pr a I Ie 245 Le u Le u G31y Le u Lys5 325 Ar g O31n Ar g As n As p 150 Thr Hi s As p A a Cy s 230 Thr A a Ph e Ser G31y 310 Le u G31y ValI A a Tr p 390 ValI Thr G31y G31y Pr a 215 As p ValI (31y ValI As n 295 G31y Pr a I Ie Ser Tyr 375 A a O31n I Ie Ser Thr 200 cys As n Ar g (31n Lys5 280 li1e A a A a O31u ValI 360 A a G31y Thr Pr a G31y 1 G31y A a cys I Ie G31y 265 ValI Le u I Ie G31y A a 345 Pr a O31u O31n O31u ValI 1 70 Ser Ph e Thr Hi s Pr a 250 (31u Hi s I Ie Ser Thr 330 Ar g Lys5 A a As n I Ie 1 O31u Lys5 Thr cys O31y 235 Thr A a ValI Thr ValI 315 Pr a As p As n O31u Ar g 395 Le u Thr O31y O31u 205 O31y O31y ValI Pr a Lys5 285 Pr a Thr O31y Thr As p 365 As n Ph e As p 1 Pr a O31y ValI As p 255 G31y As p Ser As n Thr 335 As p As n (31y <210O> 29 <21 1> 777 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CDS <222> (754) <223> RXA02542 <400> 29 ccaggct gac gcaggt gcag aaggcgct gc agat gacaat gt t gt t gacg ct gaagt t gt cgaagacgac gcagct gaca at ggt gagga caagaagt aa at g act acc cct aac 115 l'bt Thr Thr Pro Asn Page 37 seql i st cor r ect ed. t xt gga Gy acc Thr caa G n cca Pr o gat Asp gaa G u cgt Ar g gca Al a gct Al a gac Asp 150 gag G u ct c Leu t ac Tyr gat Asp ccc gac aat Pro Asp Asn gct gat cgt Al a Asp Ar g gag gaa tct G u u Ser ct c gaa gca Leu G u Al a gat ggc gat Asp G y Asp act gag gat Thr G u Asp cgt acc gag Arg Thr G u 105 gtt gtt acc Val Val Thr 120 cag cac ggt G n Hi s G y ct g at c aac Leu Ile Asn ggc gaa gca G y G u Al a 170 cag ggt gat Sn y Asp 185 ctc ggc gac Leu G y Asp 200 gag gag agc G u G u Ser cct ggg Pro y gct gag Al a G u cca ttt Pro Phe gag atc G u Ile ggt gaa G y G u 75 ctg cag Leu G n cgt gaa Arg u caa ctt G n Leu gac ctt Asp Leu 140 at c ct g I e Leu 155 ttc gat Phe Asp gtc aag Val Lys cgc gtc Arg Val cct Pr o gca Al a 30 cag G n gat Asp t cc Ser gt c Val cag G n 110 ccg Pr o gaa G u gga Gy gag G u ct g Leu 190 cgc gaa G u 15 gct Al a gcc Al a ctt Leu gct Al a acc Thr 95 ggc Gy ttg Leu ggt Gy ttg Leu at c SIle 175 gga Gy acc aat Asn gaa G u t ca Ser ct a Leu gt a Val 80 gct Al a at c SIle ct c Leu ccg Pr o aag Lys 160 cac Hi s acg Thr gca Al a 1 acc Thr gaa G u gag G u t ca Ser gaa G u gag G u at c SIle gac Asp ct g Leu 145 gt g Val gaa G u gt a Val at g Met gat Asp gca Al a gaa G u gat Asp aca Thr t ac Tyr gac Asp gat Asp 130 aag Lys gaa G u gca Al a ct c Leu gt c Val 210 cca Pr o gct Al a gaa G u gtt Val cag G n gcc Al a acc Thr 115 ctt Leu t ca Ser t cc Ser gt a Val cgc Ar g 195 ct c Leu gag G u gcc Al a at t SIle gat Asp ctt Leu aac Asn 100 gca Al a gac Asp ct g Leu ttc Phe cag G n 180 aag Lys at t SIle gca Al a cgc Ar g tct Ser cca Pr o gcc Al a t ac Tyr cgc Ar g ctg Leu tct Ser ggc G y 165 gat Asp gga Sy ggg Gy II e Ar g Thr t agagagact aagt ct ct t a gt g <210> <211> 218 <212> PRT <213> Corynebacter i um gl ut ari cum <400> IVet Thr Thr Pro Asn y IVet Pro Asp Asn Pro y Asp Pro u Asn 1 5 10 Page 38 seqI i st car r ect ed. t xt Thr Asp Pro Ou Ala Thr Ser Ala Asp Arg Ala Ou On 25 Al a Al a (3 u O31u O31u Ser O31u O31u I Ie As p Le u 145 ValI O31u ValI vb t Al a O31u ValI O31n Al a Thr 115 Le u Ser Ser ValI Ar g 1 95 Le u Ar g Ser Pr o Al a Tyr Ar g Le u Ser G31y 1 65 As p G31y G31y O31n Pr o As p 70 (31u Ar g Al a Al a As p 150 (31u Le u Tyr As p Al a (31u Le u Ar g Ar g G31y (31u 135 Ly s I Ie Ser Ar g Pr o 215 (31u (31u G31y (31u Thr 1 ValI Hi s I Ie (31u G31y 1 G31y (31u Ser Al a As p As p 90 (31u Thr G31y As n Al a 1 70 As p As p Ser Pr o (31u G31y Le u Ar g O31n As p I Ie 1 Ph e ValI Ar g O31y As n ValI Ar g Ar g Le u 125 As n O31y Pr a ValI I Ie 205 Al a Le u Al a Thr O31y Le u O31y Le u I Ie 1 O31y Thr <210O> 31 <21 1> 1977 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1954) <223> RXN02543 <400> 31 ct caat gagg agt tt t tct t accggcgaaa gt cggt ggga gt ggacagt a ct aaaat cac ct aaaacagg aggcaccat t gga att gac O3 y I I e As p ggc gag cca O31y O31u Pr o t cc gt c gt t Ser Val Val ct t gga acc acc aac tct gt g gt t Leu 03 y Thr Thr Asn Ser Val Val 15 gta gtt atc gca aac gca gaa ggc Val Val Ilie Al a Asn Al a 31u O31y 30 gca t tc gca aag aac ggt gaa gt t Al a Phe Al a Lys Asn 03 y 03 u Val 45 Page 39 agcaagt caa agct caagcc at g gga cgt gca gt a I'b t O31y Ar g Al a Val 1 t cc gt a ct t gaa ggc Ser Val Leu O31u O31y t ca cgc acc acc cct Ser Ar g Thr Thr Pr o ct a gt c ggc cag t cc Leu Val G31y O31n Ser seql i st cor r ect ed. t xt gct Al a aag Lys t ac Tyr gac Asp gtt Val ggt Sy gcg Al a 150 at t SIle gag ggu gag G u gag G u gcc Al a 230 t cc Ser gca Al a ttc Phe aac Asn gtt Val 310 aac Asn cac Hi s t ca Ser gaa G u gca Al a 120 at c SIle gca Al a gt a Val ggc Gy ggt Gy 200 ttc Phe cag G n t cc Ser aag Lys cgc Ar g 280 gtt Val ct c Leu gcg Al a ggc G y gaa Gu t ac Tyr ttc Phe ggc G y gca Al a gac Asp 170 ggt Gy gac Asp t cc Ser ct g Leu agt Ser 250 cca Pr o acc Thr aag Lys ggt Sy gtc acc aac gtt gac Val Thr Asn Val Asp acc gac tgg tcc gtt Thr Asp Trp Ser Val 75 atc tcg gct cgt acc Ile Ser Ala Arg Thr 95 ctg ggc gag gac gtc Leu G y G u Asp Val 110 gag gac tca cag cgc G u Asp Ser G n Arg 125 ctt aac gtt ctg cgt Leu Asn Val Leu Arg 140 tac ggc ctt gag aag Tyr G y Leu G u Lys 155 ctc ggt ggc ggc acc Leu G y G y G y Thr 175 gtt gtt gag gtt cgc Val Val G u Val Arg 190 gac tgg gat cag cgt Asp Trp Asp G n Arg 205 tcc aac ggc att gac Ser Asn G y Ile Asp 220 cgt gag gca gct gag Arg G u Ala Ala G u 235 gca aac atc aac ctt Ala Asn Ile Asn Leu 255 ctg ttc ttg gat gag Leu Phe Leu Asp G u 270 cag gac ctc ctg gcc G n Asp Leu Leu Al a 285 gac gct ggc gtg tcc Asp Ala Gy Val Ser 300 ggt tcc acc cgt at g G y Ser Thr Arg I\et 315 cgc acc att cgc Arg Thr lie Arg gct at c gat gac Ala lle Asp Asp ctg atg aag ctg Leu IVet Lys Leu act gat gct gtt Thr Asp Al a Val 115 cag gca acc aag G n Ala Thr Lys 130 att gtt aac gag Ile Val Asn u 145 ggc gag cag gag Sy u n G u 160 ttc gac gtc tcc Phe Asp Val Ser gca acc tcc ggc Al a Thr Ser G y 195 atc gtt gac tgg lie Val Asp Trp 210 ctg acc aag gac Leu Thr Lys Asp 225 aag gca aag atc Lys Ala Lys Ile 240 cct tac atc acc Pro Tyr Ile Thr acc ctt tcc cgt Thr Leu Ser Arg 275 cgc acc aag act Arg Thr Lys Thr 290 gtc tcg gag at c Val Ser G u lie 305 cct gct gtt acc Pro Ala Val Thr 320 t cc Ser aag Lys aag Lys 100 at t SIe gaa G u cca Pr o cag G n ct c Leu 180 gat Asp ct g Leu aag Lys gag G u gtt Val 260 gcc Al a cct Pr o gac Asp gaa G u 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page seql i st cor r ect ed. t xt gtc aag gaa ctg acc ggt gga cgt Val Lys G u Leu Thr G y Gy Arg gag G u gat Asp ggc G y ggc G y acc Thr 390 aac Asn gca Al a cct Pr o gcc Al a gaa G u 470 at t SIle aag Lys t ac Tyr gac Asp ct c Leu 550 aac Asn gct Al a gtt Val gt c Val 360 gag Su at c SIle cct Pr o gcc Al a cca Pr o 440 ggc Gy acc Thr cgc Ar g cgc Ar g acc Thr 520 aag Lys ggc G y gag Su ggt Sy 330 gca Al a gat Asp aag Lys acc Thr gtt Val 410 aag Lys ggc Gy gt c Val acc Thr at c SIle 490 gag G u aag Lys aag Lys gac Asp cag G n 570 acc Thr gtt Val gtt Val ggt G y aag Lys 395 cag G n ct g Leu gt c Val cac Hi s at t SIle 475 aag Lys cag G n tt c Phe gt c Val ct c Leu 555 gaa G u cag G n ggt G y ctt Leu ggc G y 380 cgt Ar g at c SIle ct c Leu cca Pr o gt c Val 460 cag G n gat Asp gaa G u gtt Val gaa G u 540 gag G u at g Met gct Al a gca Al a ctt Leu 365 gt g Val t cc Ser cag G n gga G y cag G n 445 acc Thr gac Asp gct Al a gt c Val gaa G u 525 gag G u gca Al a ggt G y gac Asp gca Al a 350 ctt Leu at g Met gag G u gt c Val t cc Ser 430 at c SIle gca Al a ggc G y gaa G u cgc Ar g 510 gag G u gca Al a at c l Ie aag Lys gca Al a 590 cca aac aag ggt gtt Pro Asn Lys y Val 335 ctt cag gcc ggt gtt Leu G n Ala G y Val 355 gac gtc acc cca ctg Asp Val Thr Pro Leu 370 acc aag ctc atc gag Thr Lys Leu Ile G u 385 acc ttc acc acc gca Thr Phe Thr Thr Ala 400 ttc cag ggc gag cgt Phe On y u Arg 415 ttc gag ctc ggc ggc Phe G u Leu G y G y 435 gag gtc act ttc gac G u Val Thr Phe Asp 450 aag gac aag ggt act Lys Asp Lys G y Thr 465 tcc ggt ctc tcc cag Ser G y Leu Ser G n 480 gct cac gct gat gag Ala His Ala Asp G u 495 aac aac gct gag tcc Asn Asn Ala G u Ser 515 aac tcc gag aag gtc Asn Ser G u Lys Val 530 gcc aag ggc gtt gaa Ala Lys y Val G u 545 aag gct gca gtt gag Lys Ala Ala Val G u 560 gct atc tac gag gct Al a I e Tyr G u Al a 575 ggt gca gaa ggc gct Sy Ala G u G y Ala 595 Page 41 aac Asn 340 ct c Leu t cc Ser cgc Ar g gag G u gaa G u 420 at c SIle at c SIle ggc G y gat Asp gac Asp 500 ct g Leu t cc Ser gaa G u aag Lys gac Asp 580 gca Al a cca Pr o cgc Ar g ct c Leu aac Asn gac Asp 405 at c SIe gca Al a gac Asp aag Lys gaa G u 485 aag Lys gtt Val gaa G u gca Al a ct g Leu 565 gct Al a gat Asp 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 seql i st cor r ect ed. t xt gac aat gtt gtt gac gct gaa gtt gtc gaa gac gac gca gct gac aat Asp Asn Val Val Asp Al aG u Val Val u Asp Asp Ala Ala Asp Asn 600 605 610 ggt gag gac aag aag taaatgacta cccctaacgg aat G y G u Asp Lys Lys 615 <210> 32 <211> 618 <212> PRT <213> Cor ynebact eri um gl ut am cum 1939 1977 <400> 32 lMt G y 1 Ser Val Ser Arg Leu Val Thr Ile Ile Asp lIet Lys Asp Al a Al a Thr 130 Val Asn 145 Gu G n Asp Val Thr Ser Val Asp 210 Thr Lys 225 Al a Lys Tyr Ile Ar g Al a Val G y I e Asp Leu Leu Thr G y Ar g Asp Leu Val 115 Lys G u G u Ser G y 195 Tr p Asp SIle Thr 5 Gy Pr o Ser Val Asn Ar g Thr Al a Thr Thr 165 Leu Asn Val let Leu 245 Asp Gy Ser Al a Lys 70 Ty r Asp Val Gy Al a 150 I le G u G u G u Al a 230 Ser Al a G u Val Lys 55 Ar g Thr Al a Pr o G n 135 Al a Leu SIe Leu Lys 215 Leu Ser Asp Pr o Val 40 Asn Hi s Ser G u Al a 120 I le Al a Val Gy Gy 200 Phe G n Ser Lys Val Al a G n SIle G n Al a 105 Tyr Al a Leu Phe Asp 185 Gy Sn Ar g G n Asn 265 Gy 10 Val Phe Al a Gy G u 90 Tyr Phe Gy Al a Asp 170 Gy Asp Ser Leu Ser 250 Pr o Thr SIle Al a Val Thr I le Leu G u Leu Tyr 155 Leu Val Asp Ser Ar g 235 Al a Leu Thr Al a Lys Thr Asp Ser Gy Asp Asn 140 Gy Gy Val Tr p Asn 220 G u Asn Phe Asn Asn Asn Asn Tr p Al a G u Ser 125 Val Leu Gy G u Asp 205 Gy Al a SIle Leu Ser Al a Gy Val Ser Ar g Asp 110 G n Leu G u Sy Val 190 G n SIle Al a Asn Asp 270 Val G u G u Asp Val Thr Val Ar g Ar g Lys Thr 175 Ar g Ar g Asp G u Leu 255 G u Leu Ser Arg Ala G u Phe n Arg lie Thr n Asp Leu Leu Ala Arg Page 42 seql i st cor r ect ed. t xt 275 285 Sy Val Ser Val Thr Lys Th 290 Ser G u II 305 Al a Val Th Lys G y Va Al a y Va Thr Pro Le 370 Leu li e G 385 Thr Thr Al G y G u Ar Leu G y G 43 Thr Phe As 450 Lys G y Th 465 Leu Ser G Al a Asp G Al a G u Se 51 G u Lys Va 530 G y Val 545 Ala Val G Tyr G u Al G u G y Al 59 Asp Al a Al 610 <210> 33 <211> 1977 r Pro Phe Asn e r 5 u u a g y 5 p r n u r 5 u u a a 5 a Hi s Leu 325 Pr o Ar g Leu Asn Asp 405 I le Al a Asp Lys G u 485 Lys Val G u Al a Leu 565 Al a Asp Asn Val 310 Val Asp Sy Sy Thr 390 Asn Al a Pr o Al a G u 470 I le Lys Ty r Asp Leu 550 Asn Al a Asp Sy G n Val 295 Val Leu Lys G u G u Val G u Val 360 Ile G u 375 Thr Ile G n Pro Thr Al a Ala Pro 440 Asn y 455 Asn Thr Asp Arg Arg Arg G n Thr 520 Leu Lys 535 Lys G y Thr G u Al a G y Asn Val 600 Su Asp 615 Val Lys Asp Al a 300 Val Gy Gy Ser 315 Leu Thr G y G y 330 Val Ala Val y 345 Lys Asp Val Leu Thr Lys y y 380 Pro Thr Lys Arg 395 Ser Val n Ile 410 Asn Lys Leu Leu 425 Arg G y Val Pr o Ile Val His Val 460 Ile Thr Ile n 475 IVet IIe Lys Asp 490 Gu Gu n G u 505 Arg Lys Phe Val Ala Lys Val G u 540 G u Asp Leu G u 555 Ser G n u et 570 Ala Thr G n Al a 585 Val Asp Ala a u Lys Lys Thr Ar g Al a Leu 365 Val Ser G n Sy G n 445 Thr Asp Al a Val G u 525 G u Al a Sy Asp Val 605 Ar g G u Al a 350 Leu Met G u Val Ser 430 I le Al a Sy G u Ar g 510 G u Al a l Ie Lys Al a 590 Val Mt Pr o 335 Leu Asp Thr Thr Phe 415 Phe G u Lys Ser Al a 495 Asn Asn Al a Lys Al a 575 Sy G u Page 43 seqI i st car r ect ed. t xt <212> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> OIDS 101). (1954) FRXA02 543 misc-f eat ur e 1 824 n a, t c, or g misc-f eat ur e 1 830 n a, t c, or g misc-f eat ur e 1 844 n a, t c, or g VARI ANT 575 Xaa Val, Ala, VARI ANT 577 Xaa Phe, Ser, As p, o r G31y Tyr, or Cys Thr, or Al a accggcgaaa gt cggt ggga agcaagt caa agct caagcc <220> <221> VARIANT <222> 582 <223> Xaa Ser, Pr o, <400> 33 ct caat gagg agt tt t tct t gt ggacagt a ct aaaat cac ct aaaacagg aggcaccat t gga cgt gca G31y Ar g Al a gt a ValI gga at t gac ct t G3 y I I e Asp Leu gga G31y acc acc aac tct Thr Thr Asn Ser gt g gt t Val Val 15 t cc gt a ct t Ser Val Leu gaa ggc G31u (1y ggc gag cca G31y G31u Pr o t cc gt c gt t Ser Val Val gt t at c gca aac Val Ilie Ala Asn gca Al a gaa ggc t ca cgc G31u G31y Ser Ar g acc acc cct Thr Thr Pr o ggc cag t cc G3 y G3 n Se r gca t tc gca aag Al a Phe Al a Lys ggt gaa gt t G3 y G3 u ValI ct a gt c Leu Val gct aag Al a Lys aac cag gcg gt c As n a3 n Al a ValI ac c Thr aac gt t gac cgc Asn Val Asp Arg at t cgc t cc gt c IlieArg Ser Val aag Ly s cgc cac at c ggc Arg His Ilie y gac t gg t cc gt t Asp Tr p Ser Val gct Al a at c gat gac aag Il e Asp Asp Lys t ac acc t ca cag Tyr Thr Ser 03 n g aa (31u at c t cg gct cgt Ilie Ser Ala Arg acc ct g at g aag ct g Thr Leu N/t Lys Leu 95 Page 44 aag cgc Lys Ar g 1 00 seql i st cor r ect ed. t xt gac Asp gtt Val ggt Sy gcg Al a 150 at t SIle gag Su gag G u gag G u gcc Al a 230 t cc Ser gca Al a ttc Phe aac Asn gtt Val 310 gt c Val gat Asp ggc G y gaa G u gca Al a 120 at c SIle gca Al a gt a Val ggc G y ggt Gy 200 ttc Phe cag G n t cc Ser aag Lys cgc Ar g 280 gtt Val ct c Leu gaa G u gtt Val gt c Val 360 tac ctg ggc gag Tyr Leu Gy G u ttc Phe ggc G y gca Al a gac Asp 170 ggt Sy gac Asp t cc Ser ct g Leu agt Ser 250 cca Pr o acc Thr aag Lys ggt Sy acc Thr 330 gca Al a gat Asp gac Asp aac Asn 140 ggc G y ggt G y gtt Val tgg Tr p aac Asn 220 gag G u aac Asn ttc Phe gac Asp gct Al a 300 t cc Ser gga G y ggt G y ctt Leu t ca Ser 125 gtt Val ctt Leu ggc G y gag G u gat Asp 205 ggc G y gca Al a at c SIle ttg Leu ct c Leu 285 ggc G y acc Thr cgt Ar g gca Al a ctt Leu 365 gac gtc act gat gct gtt Asp Val Thr Asp Ala Val 110 115 cag cgc cag gca acc aag G n Arg G n Ala Thr Lys 130 ctg cgt att gtt aac gag Leu Arg Ile Val Asn G u 145 gag aag ggc gag cag gag G u Lys G y G u Gn G u 160 ggc acc ttc gac gtc tcc Gy Thr Phe Asp Val Ser 175 gtt cgc gca acc tcc ggc Val Arg Ala Thr Ser G y 190 195 cag cgt atc gtt gac tgg Sn Arg lie Val Asp Trp 210 att gac ctg acc aag gac Ile Asp Leu Thr Lys Asp 225 gct gag aag gca aag atc Ala G u Lys Ala Lys Ile 240 aac ctt cct tac atc acc Asn Leu Pro Tyr Ile Thr 255 gat gag acc ctt tcc cgt Asp G u Thr Leu Ser Arg 270 275 ctg gcc cgc acc aag act Leu Ala Arg Thr Lys Thr 290 gtg tcc gtc tcg gag at c Val Ser Val Ser G u lie 305 cgt atg cct gct gtt acc Arg bet Pro Ala Val Thr 320 gag cca aac aag ggt gtt G u Pro Asn Lys y Val 335 gca ctt cag gcc ggt gtt Ala Leu G n Ala G y Val 350 355 ctt gac gtc acc cca ctg Leu Asp Val Thr Pro Leu 370 Page at t SIle gaa G u cca Pr o cag G n ct c Leu 180 gat Asp ct g Leu aag Lys gag G u gtt Val 260 gcc Al a cct Pr o gac Asp gaa G u aac Asn 340 ct c Leu t cc Ser acc Thr gct Al a acc Thr acc Thr 165 ct a Leu aac Asn gt a Val at g Met ct g Leu 245 gat Asp gag G u ttc Phe cac Hi s ct g Leu 325 cca Pr o cgc Ar g ct c Leu 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 seql i st cor r ect ed. t xt ggc att gc Gy lie G 375 acc acc at Thr Thr II 390 aac cag cc Asn G n Pr gca acc gc Al a Thr Al cct gca cc Pr o Al a Pr 4L gcc aac g( Al a Asn G 455 gaa aac ac G u Asn T\ 470 att gat cc Ile Asp Ar aag cgc cc Lys Arg Ar tac cag ac Tyr G n T\ gac ctc at Asp Leu L} 535 ct c aag gs Leu Lys G 550 aac acc g Asn Thr G nct gct g( Xaa Al a G gac aat gt Asp Asn V, 6( ggt gag gc Gy Gu A 615 <210> 34 <211> 618 ag acc aag u Thr Lys c cct acc e Pro Thr ct tct gtt ro Ser Val 410 cc aac aag a Asn Lys 425 ca cgt ggc ro Arg G y 40 gc at c gt c y Ile Val cc atc acc hr Ile Thr gc at g at c g IVet lie 490 gc gag gag g u u 505 cc cgc aag hr Arg Lys 20 ag gca aag ys Al a Lys gc gag gac y G u Asp ag tcc cag u Ser G n 570 gt gca acc y Al a Thr 585 t gtt gac al Val Asp )00 ac aag aag sp Lys Lys ggt G y aag Lys 395 cag G n ct g Leu gt c Val cac Hi s at t SIle 475 aag Lys cag G n tt c Phe gt c Val ct c Leu 555 gaa G u cag G n gct Al a ggc gtg at g G y Val MVet 380 cgt t cc gag Arg Ser G u at c cag gt c Ile G n Val ct c gga t cc Leu G y Ser 430 cca cag at c Pro n Ile 445 gtc acc gca Val Thr Al a 460 cag gac ggc Sn Asp y gat gct gaa Asp Al a G u gaa gt c cgc Su Val Arg 510 gtt gaa gag Val u u 525 gaa gag gca Su G u Al a 540 gag gca at c G u Al a l e at g ggt aag IVet G y Lys gct gac gca Al a Asp Al a 590 gaa gtt gt c G u Val Val 605 acc aag ct c Thr Lys Leu 385 acc ttc acc Thr Phe Thr 400 ttc cag ggc Phe G n a y 415 ttc gag ct c Phe G u Leu gag gt c act G u Val Thr aag gac aag Lys Asp Lys 465 t cc ggt ct c Ser G y Leu 480 gct cac gct Al a Hi s Al a 495 aac aac gct Asn Asn Al a aac tcc gag Asn Ser G u gcc aag ggc Al a Lys G y 545 aag gct gca Lys Al a Al a 560 gnt atc tnc Xaa IIe Xaa 575 ggt gca gaa G y Al a u gaa gac gac G u Asp Asp atc gag Ile G u acc gca Thr Al a gag cgt Su Arg ggc ggc G y G y 435 ttc gac Phe Asp 450 ggt act G y Thr t cc cag Ser G n gat gag Asp u gag t cc Su Ser 515 aag gt c Lys Val 530 gtt gaa Val G u gtt gag Val G u gag gct G u Al a ggc gct G y Al a 595 gca gct Al a Al a 610 cgc Ar g gag G u gaa G u 420 at c SIle at c SIle ggc G y gat Asp gac Asp 500 ct g Leu t cc Ser gaa G u aag Lys gac Asp 580 gca Al a gac Asp aac Asn gac Asp 405 at c SIe gca Al a gac Asp aag Lys gaa G u 485 aag Lys gtt Val gaa G u gca Al a ct g Leu 565 gct Al a gat Asp aat Asn 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1977 t aaat gact a cccct aacgg aat Page 46 seql i st cor r ect ed. t xt <212> PRT <213> Cor ynebact eri um gl ut ari cum <220> <221> VARIANT <222> 575 <223> Xaa Val, <220> <221> VARIANT <222> 577 <223> Xaa Phe, <220> <221> VARIANT <222> 582 <223> Xaa Ser, <400> 34 IVet y Arg Al a 1 Ser Val Leu G u Ser Arg Thr Thr Leu Val Gy G n Thr Ile Arg Ser Ile Asp Asp Lys IVet Lys Leu Lys 100 Asp Al a Val I le 115 Ala Thr Lys G u 130 Val Asn u Pro 145 Gu Gn G u Gn Asp Val Ser Leu 180 Thr Ser G y Asp 195 Val Asp Trp Leu 210 Thr Lys Asp Lys 225 Ala Lys Ile G u Ala, Asp, or Gy Ser, Tyr, or Cys Pro, Thr, or Ala Val 5 Gy Pr o Ser Val Asn Ar g Thr Al a Thr Thr 165 Leu Asn Val Met Leu 245 G y I e Asp Leu Gy Ser Al a Lys 70 Tyr Asp Val Gy Al a 150 I le G u G u G u Al a 230 Ser G u Val Lys 55 Ar g Thr Al a Pr o G n 135 Al a Leu SIle Leu Lys 215 Leu Ser Pr o Val 40 Asn Hi s Ser G u Al a 120 I le Al a Val Gy Gy 200 Phe G n Ser Val 25 Al a G n SIle G n Al a 105 Tyr Al a Leu Phe Asp 185 G y G n Ar g G n Thr SIle Al a Val Thr 75 I le Leu G u Leu Tyr 155 Leu Val Asp Ser Ar g 235 Al a Thr Al a Lys Thr Asp Ser Sy Asp Asn 140 Sy Sy Val Tr p Asn 220 G u Asn Asn Asn Asn Asn Tr p Al a G u Ser 125 Val Leu Sy G u Asp 205 Sy Al a SIle Ser Al a Gy Val Ser Ar g Asp 110 G n Leu G u Sy Val 190 G n SIle Al a Asn Val G u G u Asp Val Thr Val Ar g Ar g Lys Thr 175 Ar g Ar g Asp G u Leu 255 Val Sy Val Ar g Al a Leu Thr G n SIe Sy 160 Phe Al a SIe Leu Lys 240 Pr o Tyr Ile Thr Val Asp Al a Asp Lys Asn Pro Leu Phe Leu Asp G u Thr Page 47 seqi i st car r ect ed. t xt 265 Le u Thr Ser 305 A a Ly s A a Thr Le u 385 Thr O31y Le u Thr Ly s 465 Le u A a A a O31u O31y 545 A a Xaa O31u Ar g 275 Thr I Ie Thr ValI ValI 355 Le u O31u A a Ar g O31y 435 As p Thr O31n O31u Ser 515 ValI O31u O31u A a A a 595 O31u Ph e Hi s Le u 325 Pr o Ar g Le u As n As p 405 li1e A a As p Ly s O31u 485 Ly s ValI O31u A a Le u 565 A a As p Ph e As n ValI 310 ValI As p O31y O31y Thr 390 As n A a Pr o A a O31u 470 li1e Ly s Tyr As p Le u 550 As n Xa a As p O31n (31n 295 ValI Ly s O31u O31u I Ie 375 Thr O31n Thr A a As n 455 As n As p Ar g O31n Le u 535 Ly s Thr A a As n Hie Thr 0On Asp ValI ValI Le u ValI 345 Ly s Thr Pr o Ser As n 425 Ar g I Ie I Ie vb t O31u 505 Ar g A a O31u Ser A a 585 ValI Ly s O31y Thr 330 A a As p Ly s Thr ValI 410 Ly s O31y ValI Thr I Ie 490 O31u Ly s Ly s As p O31n 570 Thr As p As p O31y 315 O31y ValI ValI O31y Ly s 395 O31n Le u ValI Hi s I Ie 475 Ly s O31n Ph e ValI Le u 555 O31u O31n A a A a 300 Ser O31y O31y Le u O31y 380 Ar g I Ie Le u Pr o ValI 460 O31n As p O31u ValI O31u 540 O31u vb t A a aOu Leu Leu 285 03 y Val Thr Ar g Ar g O31u A a A a 350 Leu Leu 365 Val [Vb t Ser 03 u O3 n Val O3 y Ser 430 O31n I Ie 445 Thr A a As p (1y A a 03 u Val Ar g 510 O31u (1u 525 O3 u A a A a I I e O3 y Lys As p A a 590 Val Val 605 A a Ser vb t Pr a 335 Le u As p Thr Thr Ph e 415 Ph e O31u Ly s Ser A a 495 As n As n A a Ly s Xaa 575 O31y aOu Asp A a A a Asp Asn 03 y 03 u Asp Lys Lys 610 615 Page 48 seqi i st car r ect ed. t xt <210O> <21 1> 1947 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1924) <223> RXN02280 <400> cgcgat tgcg t cat cgat cg t tgt tgct tc cat gcgcacc ct gat gccct gt ggat t caa aact gt gct t t t at aggcgt acact at ctt t ct gcacgcc cgt Ar g cgc Ar g aat As n t ac Tyr tt t Ph e g aa O31u ct g Le u t gt CYS gcg A a gag O31u 1 50 gt g ValI t cc Ser aat As n at t I Ie gt t ValI ccg Pr o ct g Le u ct g Le u cgg Ar g at g vb t 120 gcg A a ctt Le u ct g Le u gt c ValI c aa O31n t cc Ser gct A a at t I Ie g at As p ac g Thr ggt G31y gcg A a cgg Ar g g at As p ccg Pr o 1 70 at t I Ie gt t ValI ggt G31y t gt Cy s cgt Ar g aat As n 75 gt g ValI cgc Ar g g at As p t gg Tr p gac As p 155 cgc Ar g gct A a g ac As p ccg Pr o act Thr at t I Ie ggt G31y G31y ct g Le u gag O31u act Thr 140 gca A a cct Pr o agt Ser ggc G31y gt g ValI cgt Ar g ccg Pr a O31y ac g Thr c aa O31n 110 ac c Thr cat Hi s g at As p gag O31u t at Tyr 1 90 gt t ValI gt g ValI g aa O31u ac c Thr ac c Thr 80 aaa Ly s ggc O31y gt g ValI g at As p at t I Ie 1 60 ac g Th r cgc Ar g at g vb t 1 gt t ValI cgt Ar g c ag O31n aag Ly s gcg A a cgc Ar g at c I Ie t cg Ser ggc G31y 145 ccg Pr a tt g Le u t ac Tyr g at As p gag (31u ggt G31y g at As p c ag O31n g at As p ggg O31y cat Hi s 130 ac c Thr gt g ValI ct g Le u ct g Le u caa gaa tcc 03 n 03 u Se r ct t Le u tt g Le u gag O31u cgt Ar g gag O31u g aa O31u ct g Le u 115 gcg A a tt t Ph e ggc O31y ac g Thr ccg Pr a 1 tt g Le u ct g Le u gag O31u gcc A a gcg A a tt c Ph e 1 00 ct t Le u gag O31u aac As n ac g Thr g aa O31u 1 at t I Ie t ca Ser agt Ser c ag O31n O31y ac g Thr cgg Ar g ggt O31y agt Ser ggt O31y ct g Le u act Thr 1 aat As n cct Pr a at t gt g gt g cag ggt gag lieVal Val On Oy Ou aaa aac acc acc atc act aca t cg ccg gt g Ser Pr o Val Lys Asn Thr Thr I I e Thr Thr Page 49 INO seql i st cor r ect ed. t xt 200 205 210 ttt gca aag gat act gat cag cag cac agg ctg tat gcc ggc cgg gag 787 Phe Al a Lys Asp Thr Asp G n G n His Arg Leu Tyr Al a G y Arg G u 215 220 225 d) 14 cgc ctt ggt aaa act cct ttt gat gtc atc gat ctc acc ggt cct ggc 835 Arg Leu y Lys Thr Pro Phe Asp Val lie Asp Leu Thr y Pro y 230 235 240 245 atc gag ggt gtg gct tat gta ttg ccg gag gcc cag gct ccg cat atg 883 Ile G u y Val Ala Tyr Val Leu Pro u Ala n Ala Pro His Met 250 255 260 00 tcc agg cgt cac agt att tat gtc aac cgc atg ttg gtc tct gat ggg 931 SSer Arg Arg His Ser I e Tyr Val Asn Arg IVet Leu Val Ser Asp G y 265 270 275 (N cct tcc acg gtg ctg ccc aac tgg gcg ttc ttt gtg gaa tgt gaa atc 979 0 Pro Ser Thr Val Leu Pro Asn Trp Ala Phe Phe Val G u Cys G u Ile 280 285 290 (N aat tca acc gat ttg gaa ccc acc gca tcg cgt gaa gcg ctc atg gat 1027 Asn Ser Thr Asp Leu G u Pro Thr Ala Ser Arg G u Ala Leu IVet Asp 295 300 305 gac acc gcg ttc gcg gca acc agg gaa cat atc ggt gag tgc att aaa 1075 Asp Thr Al a Phe Al a Al a Thr Arg G u His lie G y G u Cys IIe Lys 310 315 320 325 tcg tgg ctg att aat ctc gcc atg acc aag cct cac cgc gtg cgg gaa 1123 Ser Trp Leu Ile Asn Leu Ala IVet Thr Lys Pro His Arg Val Arg G u 330 335 340 ttt act gcg att cat gat ctt gcc ctg cgc gag ctg tgc caa tcg gac 1171 Phe Thr Ala lie His Asp Leu Ala Leu Arg G u Leu Cys G n Ser Asp 345 350 355 gcg gac ctg gct gaa acc atg ttg ggt ctt ctc acc ttg gag acc tcc 1219 Al a Asp Leu Al a G u Thr IVet Leu G y Leu Leu Thr Leu G u Thr Ser 360 365 370 cgt ggt cgc atc tcg atc ggt gag atc acc acg ttg tcc atc acc gag 1267 Arg y Arg lie Ser lie y u lie Thr Thr Leu Ser Ile Thr u 375 380 385 gat gtg tcg ctg cag ctg gct acc acg ttg gat gat ttc agg cag ctc 1315 Asp Val Ser Leu G n Leu Ala Thr Thr Leu Asp Asp Phe Arg G n Leu 390 395 400 405 aac acc att gcg cgc ccg gac acc ttg att att aat ggc ggc tac att 1363 Asn Thr lie Ala Arg Pro Asp Thr Leu lie lie Asn Gy y Tyr Ile 410 415 420 cac gac agc gat ctg gct cgg ctc att ccc gtt cac tac cca ccg ctt 1411 His Asp Ser Asp Leu Ala Arg Leu Ile Pro Val His Tyr Pro Pro Leu 425 430 435 acg gta tct act gct gac ctg cgc gaa tcc atg gat ctg atg gag ctt 1459 Thr Val Ser Thr Ala Asp Leu Arg u Ser IVet Asp Leu IVet G u Leu 440 445 450 ccg ccg ctg cag gac att gag aaa gcc aag gca ctg gat gcg cag gtc 1507 Pro Pro Leu G n Asp Ile G u Lys Ala Lys Ala Leu Asp Ala G n Val 455 460 465 acg gaa tca ttg aag gat ttt cag atc aag ggc gca acg agg gtt ttt 1555 Thr u Ser Leu Lys Asp Phe n Ile Lys y Ala Thr Arg Val Phe Page INO seql i st cor r ect ed. t xt 470 475 480 485 C- gaa ccc gca gat gtt cct gcc gtg gtg atc att gat tcc aag gcg cag 1603 G u Pro Ala Asp Val Pro Ala Val Val Ile Ile Asp Ser Lys Ala G n 490 495 500 d) 4 gcc tca cgg gat cgc aat gaa aca caa agc gca acc act gat cgt tgg 1651 Ala Ser Arg Asp Arg Asn G u Thr G n Ser Ala Thr Thr Asp Arg Trp -T 505 510 515 gct gac att ttg gca acg gtg gat aac acg ttg agc cgt caa aca gcc 1699 Ala Asp Ile Leu Ala Thr Val Asp Asn Thr Leu Ser Arg G n Thr Ala 520 525 530 00 aac att cca cag gat cag gga ctg tcg gcg ttg tgc ttg aat tgg aac 1747 0 Asn Ile Pro G n Asp G n y Leu Ser Ala Leu Cys Leu Asn Trp Asn 535 540 545 (N aat tcg ctg gtc agg aaa ttg gcg tcc act gat gac acc gcc gtg gtg 1795 0 Asn Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val S550 555 560 565 (N tcg cgc acg gtg cgt ttg ctc tac gtt cag gca ttg ttg tcc agc aag 1843 Ser Arg Thr Val Arg Leu Leu Tyr Val G n Ala Leu Leu Ser Ser Lys 570 575 580 agg cca ctg cgg gtg aag gaa cgc gcg ctg ctt aat gat tcg ctg gca 1891 Arg Pro Leu Arg Val Lys G u Arg Ala Leu Leu Asn Asp Ser Leu Ala 585 590 595 gat ctg gtt tct ttg tct ttg tca tcc gat atc taagacaatc ctccgctaat 1944 Asp Leu Val Ser Leu Ser Leu Ser Ser Asp Ile 600 605 ctt 1947 <210> 36 <211> 608 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 36 IVet G n u Ser Ser Arg Asp Asn Phe G n Val Asp Leu y y Val 1 5 10 Val Asp Leu Leu Ser Arg His lie Tyr Ser Gy Pro Arg Val Tyr Val 25 Arg G u Leu Leu G n Asn Ala Val Asp Ala Cys Thr Ala Arg Ser G u 40 On Gy u u y Tyr u Pro Ser lie Arg lie Arg Pro Val Thr 55 Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn y Thr y Leu Thr 70 75 Ala n u Ala Arg u Leu Leu Ala Thr Val y Arg Thr Ser Lys 90 Arg Asp G u Phe G y Leu On Arg u y Arg Leu G y n Phe G y 100 105 110 I e G y Leu Leu Ser Cys Phe bet Val Al a Asp G u I e Thr bet Val 115 120 125 Ser His Ala G u G y Al a Ser Ala IIe Arg Trp Thr G y His Ala Asp Page 51 seqI i st car r ect ed. t xt 140 A a Thr Asp Val G31y 145 Pr o Le u Tyr Thr Tyr 225 Le u O31n Le u ValI O31u 305 G31y Hi s Le u Thr Le u 385 As p As n Hi s As p Le u 465 Thr Phe Asn Leu (31y Thr Pr o 1 95 Ser G31y G31y Pr o Ser 275 cys Le u cys ValI O31n 355 O31u I Ie Ar g O31y Pr o 435 vb t A a Thr 1 65 As n Pr o ValI O31u O31y 245 vb t Oy le As p Ly s 325 O31u As p Ser O31u Le u 405 li1e Le u Le u ValI O31u 150 ValI Ser I Ie Ph e Ar g 230 li1e Ser Pr a As n As p 310 Ser Ph e A a Ar g As p 390 As n Hi s Thr Pr a Thr 470 Il e Leu 0 yAsp Hi s ValI ValI A a 215 Le u O31u Ar g Ser Ser 295 Thr Tr p Thr As p O31y 375 ValI Thr As p ValI Pr a 455 O31u Le u ValI ValI 200 Ly s O31y O31y Ar g Thr 280 Thr A a Le u A a Le u 360 Ar g Ser I Ie Ser Ser 440 Le u Ser Thr Thr 1 85 O31n As p Ly s ValI Hi s 265 ValI As p Ph e I Ie I Ie 345 A a I Ie Le u A a As p 425 Thr O31n Le u As p 1 Ar g A a O31u As p Pr a 235 Tyr I Ie Pr a O31u A a 315 Le u As p Thr I Ie Le u 395 Pr a A a As p I Ie As p 475 Pr o Ser Ly s O31n 220 Ph e ValI Tyr As n Pr a 300 Thr A a Le u vb t O31y 380 A a As p Ar g Le u O31u 460 Ph e As p As n As n 205 O31n As p Le u ValI Tr p 285 Thr Ar g vb t A a Le u 365 O31u Thr Thr Le u Ar g 445 Ly s O31n (31u Tyr 1 Thr Hi s ValI Pr a As n 270 A a A a O31u Thr Le u 350 O31y I Ie Thr Le u I Ie 430 O31u A a I Ie Ar g 1 O31y Thr Ar g I Ie O31u 255 Ar g Ph e Ser Hi s Ly s 335 Ar g Le u Thr Le u I Ie 415 Pr a Ser Ly s Ly s Ala Thr Arg Val Phe 0 u Pro Al aAsp Val 485 490 Pro Ala Val Val Ilie Ilie 495 Page 52 As p Thr Ser cys 545 As p Le u As n Ly s As p 515 O31n As n A a Ser Ser 595 O31n Tr p A a As n ValI 565 Ly s A a A a A a As n As n 550 Ser Ar g As p Ser As p I Ie 535 Ser Ar g Pr a Le u seql i st car r ect ed. t xt Ar g Asp Ar g As n 03 u Th r 505 li1e Leu Ala Thr Val As p 520 525 Pro O31n As p O31n O31y Leu 540 Leu Val Ar g Lys Leu Ala 555 Thr Val Ar g Leu Leu Ty r 570 Leu Ar g Val Lys O31u Ar g 585 Val Ser Leu Ser Leu Ser 600 605 O31n 510 As n Ser Ser ValI A a 590 Ser Ser Thr A a Thr O31n 575 Le u As p <210O> 37 <21 1> 436 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ari cumn <220> <221 ODS <222> (436) <223> FRXA02282 <400> 37 cgcgat tgcg t cat cgat cg t tgt tgct tc cat gcgcacc ct gat gccct gt ggat t caa aact gt gct t t t at aggcgt cgt Ar g cgc Ar g aat As n t ac Tyr tt t Ph e g aa O31u ct g Le u aat As n at t I Ie gt t ValI ccg Pr a ct g Le u ct g Le u cgg Ar g c aa O31n t cc Ser gct A a at t I Ie g at As p ac g Thr ggt O31y g ac As p ccg Pr a act Thr at t I Ie ggt O31y O31y ct g Le u ct c Le u ag g Ar g gca A a 45 cgg Ar g ac g Thr cgg Ar g ggg O31y ggc O31y gt g ValI cgt Ar g ccg Pr a O31y ac g Thr c aa O31n 110 gt t ValI gt g ValI g aa O31u ac c Thr ac c Thr 80 aaa Ly s ggc O31y acact at ct t t ct gcacgcc at g caa gaa t cc t ca l'b t 03 n 03 u Ser Ser 1 gt t gat ct t t tg agt Val As p Leu Leu Ser cgt gag t tg ct g cag Ar g O31u Leu Leu O31n cag ggt gag gag ggc O31n (1y (1u (1u (1y aag gat cgt gcc acg Lys Asp Ar g Ala Thr gcg cag gag gcg cgg Ala O31n O31u Ala Ar g cgc gat gaa t tc ggt Ar g Asp O31u Phe O31y 1 00 Page 53 seql i st cor r ect ed. t xt <210> 38 <211> 112 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 38 IVet G n u Ser Ser Arg Asp Asn Phe 1 5 Val Asp Leu Leu Ser Arg His lie Tyr Arg G u Leu Leu G n Asn Ala Val Asp 40 Sn G y G u G u G y Tyr u Pro Ser Lys Asp Arg Ala Thr Phe Ser Leu Val 70 Ala Gn G u Ala Arg G u Leu Leu Al a Arg Asp u Phe y Leu n Arg u 100 105 G n 10 Ser Al a SIle Asp Thr 90 Sy Val Sy Cys Ar g Asn Val Ar g Leu Ar g Al a Ar g Thr Ar g Sy Sy Tyr Ser Val Leu Ser Phe <210> 39 <211> 1269 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1246) <223> RXA00886 <400> 39 taagtatgtt ggtcgtgtgc tcgctggcga atagctgcgg tgattaatct gtttagaagc taaaggaagt atcacccacc t ac Tyr aag Lys ggt Sy gt a Val cca Pr o ttc Phe at t SIle t ac Tyr gaa G u act Thr gag Gu ggc G y ggc G y aag Lys gcg Al a ccg Pr o ggc Gy ggt Sy gt c Val ctt Leu gag G u gat Asp ggc Gy 75 gga G y gat Asp gcc Al a aaa Lys aag Lys gga Gy ct g Leu cgc Ar g cgc Ar g ttc Phe 45 cgc Ar g gct Al a ggc Gy aat Asn aaa Lys cgc Ar g cgc Ar g ggc Gy gat Asp gca acc Al a Thr 15 tac cac Tyr Hi s gag gct G u Al a att gtt Ile Val gct ggt Al a y 80 atc ttc I e Phe 95 Page 54 gtatagttgg ccatttgttt gtg gca cgt gac tat Val Ala Arg Asp Tyr 1 gaa tca gag atc aaa Su Ser G u Ile Lys ccg gac gta aac cca Pro Asp Val Asn Pro tct gtt gcg cat gag Ser Val Ala His G u gat atg ggc ggt gac Asp bet G y y Asp ggc ttc ggt gga ggc Gy Phe Gy Gy Gy gat gcc ttc ttc ggc Asp Ala Phe Phe G y 100 seql i st cor r ect ed. t xt ggt Gy agt Ser ggc G y tgt Cys 150 acc Thr ggc G y gag G u cgt Ar g ggc Gy 230 ggt Gy at c SIle at g Met ggc G y gt g Val 310 cac Hi s gat Asp gac Asp gcg Al a acc Thr 120 aag Lys ggt Sy aat Asn gt c Val at c SIle 200 cgc Ar g cgc Ar g gcg Al a acc Thr gat Asp 280 gag Su acc Thr aac Asn cgc Ar g gct Al a 360 ggt tcc cgt gga Sy Ser Arg y cca tgg Tr p gat Asp gga G y gct Al a 170 acg Thr gat Asp gac Asp cgc Ar g gac Asp 250 gat Asp gcg Al a aaa Lys gat Asp at g Met 330 cgc Ar g gt g Val cgc Ar g ct c Leu tct Ser 155 ggt G y t cc Ser cct Pr o at c SIle at g Met 235 ct c Leu ggc Gy ctt Leu at t SIle ggt G y 315 gcg Al a gaa G u cat Hi s acc Thr acc Thr 140 gca Al a gaa G u cgc Ar g tgc Cys gt g Val 220 gca Al a t ac Tyr gac Asp ggc G y acc Thr 300 gaa G u cat Hi s ttg Leu cgc Ar g t cc Ser 125 ctt Leu t cc Ser at t SIle cca Pr o act Thr 205 gcc Al a ggc Gy at t SIle gat Asp acc Thr 285 at c SIle ggc G y gt c Val ctt Leu gaa G u 365 Pr o 110 at c SIle gac Asp gac Asp cag G n tgc Cys 190 gag G u aac Asn caa Gn gaa ct g Leu 270 gaa G u cct Pr o at g Met gat Asp gaa G u 350 ggc Gy cgt tcc cgc gtg cag Arg Ser Arg Val G n 115 acc ttg gaa gag gct Thr Leu G u G u Ala 130 acc gca gtg ctg tgt Thr Al a Val Leu Cys 145 aag aag cct gtt acc Lys Lys Pro Val Thr 160 gaa gtg cag cgc agc G u Val G n Arg Ser 175 cac acc tgc gat ggc His Thr Cys Asp G y 195 tgt gca gca gat ggt Cys Al a Al a Asp G y 210 atc cca gct ggc at c Ile Pro Al a G y Ile 225 ggt gag gtt ggc gct Gy Gu Val Gy Ala 240 gtc atg gtg cgc ccg Val IVet Val Arg Pro 255 cac gcc agc atc aag His Ala Ser Ile Lys 275 ttg gac gtg gaa t cc Leu Asp Val G u Ser 290 gca ggt act cag ccc Ala y Thr G n Pro 305 ccg aag ctg cgc gca Pro Lys Leu Arg Ala 320 cta ttt gtg cca acc Leu Phe Val Pro Thr 335 gaa atc cgc aac cat Su lie Arg Asn His 355 gga gaa gaa t cc ggt G y G u u Ser G y 370 Page cca Pr o t ac Tyr acc Thr tgt Cys ttc Phe 180 acc Thr cgt Ar g cag G n ggt Gy cac Hi s 260 gtt Val ct c Leu aac Asn gaa G u gat Asp 340 cgc Ar g ttc Phe ggc G y aag Lys aag Lys ggc G y 165 ct g Leu ggt G y gt g Val t cc Ser ggc Gy 245 gcc Al a cca Pr o acc Thr gat Asp ggc G y 325 ttg Leu agc Ser ttt Phe 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 seql i st cor r ect ed. t xt gac aag ctc cga aac aag ttc cgc aaa taatgtcact gccagtattt Asp Lys Leu Arg Asn Lys Phe Arg Lys 375 380 at c <210> <211> 382 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1266 1269 <400> Val Ala 1 Su Ser Pro Asp Ser Val Asp Ibet G y Phe Asp Al a Arg Val G u G u 130 Val Leu 145 Pro Val G n Ar g Cys Asp Al a Asp 210 Al a G y 225 Val G y Val Arg Arg Asp 3 u Ile Val Asn Al a Hi s (y Gy (3 y G y Phe Phe 100 3 n Pro 115 Al a Tyr Cys Thr Thr Cys Ser Phe 180 y Thr 195 3 y Arg Ile G n Al a G y Pro Hi s 260 Tyr 5 Lys Pr o G u Asp Gy Gy Gy Lys Lys Gy 165 Leu Gy Val Ser Gy 245 Al a Tyr y Ile Leu Al a G u Leu 55 Met Gy Gy Asp Al a 135 Hi s Cys Asn SIle Al a 215 Mt Pr o Phe Tyr G u 40 Thr Gu Gy Al a Thr 120 Lys Gy Asn Val SIle 200 Ar g Ar g Al a Thr Asp 280 Ar g Al a Asp Gn Ser Gy 105 Leu Lys Ser Gy et 185 Pr o Ar g SIle Gy Ar g 265 Gy 10 Lys Al a Pr o Gy Gy 90 Gy Tr p Asp Gy Al a 170 Thr Asp Asp Ar g Asp 250 Asp Val Leu G u Asp Gy Gy Ser Ar g Leu Ser 155 Gy Ser Pr o SIle Met 235 Leu Gy Asp Al a Lys Lys Gy Leu Ar g Thr Thr 140 Al a G u Ar g Cys Val 220 Al a Tyr Asp Ar g Ar g Phe Ar g Al a Gy Gy Ser 125 Leu Ser SIle Pr o Thr 205 Al a Gy I e Asp Al a Tyr Su I e Al a SIe Ar g Thr Thr Lys G u 175 Hi s Cys SIe Gy Val 255 Hi s Ser Ile Lys 275 Val Pro Iet Phe Al a Al a Leu G y Thr G u Leu Asp 285 Page 56 seqi i st car r ec Val O31u Ser Leu Thr Gy Ou Ou Val Lys I Ie 290 295 Thr O31n Pr o As n As p Val I Ie Thr Leu As p O31y 305 310 315 Leu Ar g Ala O31u (31y His G31y As n Leu l'b t Ala 325 330 Val Pr o Thr As p Leu As p As p Ar g Thr Ar g O31u 340 345 Ar g As n Hi s Ar g Ser As p As n Ala Ser Val Hi s 355 360 O31u Ser G31y Phe Phe As p Lys Leu Ar g As n Lys 370 375 <210O> 41 <21 1> 1470 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1447) <223> RXS00568 <400> 41 gt t tcggacg acgcgagaaa t cgcat taat cgt cggaaac ct gaaact tt ccct t tcccg at cat ccagg agat t tact c ted. t xt Thr I Ie 300 O31u (1y Hi s Val Leu Leu Ar g O31u 365 Phe Ar g 380 A a 03 y Pr o Lys 320 Leu Phe 335 O31u I Ie O31y (1u cgggct tgt t t t tgtaat at gt g aag agt t ct gt c 11! Val Lys Ser Ser Val gag O31u t ct Ser c aa O31n at c I Ie g ac As p aag gag O31u ct t Le u ct g Le u ct g Le u c ag O31n gca A a ct t Le u at c I Ie gt t ValI aag Lys5 120 g ac As p cca Pr a cct Pr a tt c Ph e aac As n c ag O31n tt c Ph e gag (31u cgt Ar g at c I Ie tt c Ph e cgt Ar g t ac Tyr aac As n gct A a at c I Ie t ca Ser g ac As p cgt Ar g 45 ggt O31y gca A a gt a ValI gag O31u act Thr 125 aag Lys5 c ag O31n aag Lys5 gcg A a c ag O31n gag O31u gt t ValI 110 gt t ValI at c lIe 15 gca A a ggc O31y gt t ValI gca A a gt c ValI 95 g ac As p gag O31u ac c Thr t ac Tyr aag Lys5 ct g Le u at c I Ie ac c Thr gt t ValI gt c ValI 1 gt t ValI gcc A a gca A a gag O31u g aa O31u aag Ly s cgc Ar g cca Pr a gag O31u gct A a ccg Pr a c ag O31n gct A a at c I Ie cca Pr a gct A a 130 cca Pr a gcg A a c ag O31n gt c ValI ggc O31y g ac As p 1 00 tt c Ph e aag Ly s Page 57 seql i st cor r ect ed. t xt gac gaa gag gca atc gaa gca gag Asp Gu Gu Ala lle G u Ala G u ctc gag Leu G u acc ctg cgt gca cgt ttc Thr Leu Arg Ala Arg Phe 135 t cc acc Ser Thr 150 acc at c Thr Ile acc act Thr Thr ggc ctg G y Leu ttc acc Phe Thr 215 atc agc I e Ser 230 gat gat Asp Asp ctt cgt Leu Arg cag gct G n Al a gct gac Al a Asp 295 cag ctg G n Leu 310 aac t cc Asn Ser aag aac Lys Asn gac acc Asp Thr acc gac Thr Asp 375 cag ttc G n Phe 390 ttg Leu aac Asn gag G u gac Asp 200 tct Ser gtt Val gag G u gag G u gct Al a 280 ttc Phe cac Hi s ct c Leu gt c Val ct c Leu 360 cac Hi s at c SIle gat Asp acc Thr 170 ct g Leu gct Al a ct g Leu at c SIle gca Al a 250 acc Thr at c l Ie ct g Leu ct c Leu gag G u 330 gat Asp gag G u ct g Leu cag G n cac Hi s 155 gca Al a t cc Ser ct g Leu gca Al a acc Thr 235 cag G n gt g Val cgc Ar g cca Pr o ct c Leu 315 gct Al a gct Al a gtt Val tt c Phe ct g Leu 395 140 aac Asn agc Ser t ac Tyr at c SIle aac Asn 220 gca Al a ct g Leu tct Ser gac Asp cag G n 300 ggc Sy cag G n gag G u gag G u acc Thr 380 cag G n cac Hi s at t SIle gaa G u ggc G y 205 ggc G y acc Thr gct Al a gac Asp gaa G u 285 t cc Ser gag G u ggc G y aag Lys gag G u 365 gca Al a cag G n aag Lys gac Asp at c SIle 190 gct Al a gag G u aag Lys tct Ser gtt Val 270 gtt Val at c SIle ctt Leu acc Thr gct Al a 350 cct Pr o cag G n t cc Ser aag Lys 160 gag G u tct Ser aag Lys aag Lys cgc Ar g 240 ttc Phe gct Al a gct Al a gac Asp cac Hi s 320 cgt Ar g cgc Ar g gtt Val t ac Tyr cag G n 400 ggt Gy at t SIle gat Asp gaa G u 210 aag Lys ct g Leu acc Thr cag G n gct Al a 290 cag G n gat Asp gag G u cag G n cag G n 370 at g Met gcg Al a gag G u gaa G u ct g Leu 195 acc Thr gaa G u cct Pr o at c SIle aag Lys 275 ctt Leu gca Al a gct Al a ttc Phe ct g Leu 355 cag G n gac Asp aac Asn ttc Phe gag G u 180 at t SIle gt a Val gct Al a gag Gu gaa G u 260 aac Asn ggc Sy cac Hi s gca Al a gac Asp 340 ttc Phe gag G u cca Pr o ct c Leu gt c Val 165 gca Al a gac Asp gag G u caa G n ct g Leu 245 gag G u gag G u gag G u t cc Ser ct g Leu 325 aag Lys ct g Leu ct c Leu aac Asn ttc Phe 405 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 Page 58 tcc gac gtt cgc cgt ggc aag gct c Ser Asp Val Arg Arg G y Lys Ala L 410 aac gtg aag gac tcc gag ggt aac g Asn Val Lys Asp Ser G u G y Asn C 425 4 ggt gaa gaa gaa gta gct gag act g Gy u u u Val Ala u Thr C 440 445 aaagaaat aa cgc <210> 42 <211> 449 <212> PRT <213> Cor ynebact eri um gl ut ari cum 1 i st cor r ect ed. t xt t t gca cag gct atc tgc cgc gta .eu Ala n Ala lie Cys Arg Val 415 420 lag atc gac cct aag gaa tac ttc Su I e Asp Pro Lys G u Tyr Phe .30 435 lag tct gaa gct taaaaacttt Su Ser G u Ala 1363 1411 1457 1470 <400> 42 Val Lys Ser Ser Val G u Lys Leu Ser 1 Val Al a Al a G u G u Lys Ar g Pr o Leu 145 Lys Lys Asp Asp Gy 225 Val Leu Ar g Val G u G u G u 115 I le Al a G u G u Leu 195 Thr G u 5 Phe G n Leu Asn SIle Asn G u Al a Phe Val 165 Al a Asp G u G n G u Val Asp lbet Al a Leu Pr o G u 135 Thr SIle Thr Leu Thr 215 Ser Leu G n 40 Al a Leu l Ie Val Lys 120 G u Leu Asn G u Asp 200 Ser Val Lys I e Ar g Pr o Gy G u 105 Phe Al a Lys SIle Gy 185 Lys G u G u Asp 10 Pr o Pr o Phe Asn G n 90 Phe Su l e Asp Thr 170 Leu Al a Leu SIle Thr G u Gy Gy Ar g Pr o Val Asp G u Hi s 155 Al a Ser Leu Al a Thr 235 Ar g SIle Phe Ar g Tyr Asn Al a SIle Al a 140 Asn Ser Tyr SIle Asn 220 Al a Ser Asp Ar g Gy Al a Val G u Thr 125 G u Hi s SIle G u Gy 205 Gy Thr Lys G n Lys Al a G n G u Val 110 Val Leu Lys Asp SIle 190 Al a G u Lys I e Al a Sy Val Al a Val Asp G u G u Leu Sy 175 Sy Lys Hi s G n G u Leu Pro G u Leu Asp Asp G u Phe Ala G n Leu Ala Ser G u Phe 245 255 Page 59 seqI i st car r ect ed. t xt Asp Thr Ilie Ou 260 (31u Le u Ar g (31u Se r Thr Val Ser Asp Val (31u Ala 270 Ly s A a (31u 305 As p (31u Thr Ser G y 385 li1e A a Pr o A a Ly s 275 Le u A a A a Ph e Le u 355 O31n As p As n cys O31u 435 O31u O31u Ser Le u 325 Ly s Le u Le u As n Ph e 405 ValI Ph e O31n A a O31n 310 As n Ly s As p Thr G n 390 Ser As n G31y A a As p 295 Le u Ser As n Thr As p 375 Ph e As p ValI (31u A a 280 Ph e Hi s Le u ValI Le u 360 Hi s I Ie ValI Ly s (31u 440 265 A a A a O31n Le u O31u 345 Ser I Ie G y Ar g As p 425 (31u Ar g Pr o Le u 315 A a A a ValI Ph e Le u 395 O31y O31u A a O31u 285 Ser O31u O31y Ly s O31u 365 A a G n A a As n Thr 445 Le u ValI A a Thr 335 ValI O31u Ser G y A a 415 li1e Ser <210O> 43 <21 1> 826 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (826) <223> RXN03038 <400> 43 gcgcggaaaa caccaagtaa gccttacagt ccaaacacgt t caaat ccgt t aaagt gcct gag t ta aca gt g cgt aaa gga att t O31u Leu Thr Val Ar g Lys O31y I Ie S gct agt tca at c gga t tc gga act g Ala Ser Ser Ilie O31y Phe O31y Th r\ gca gct caa gac t ct gca ttt gac t A a A a 03 n Asp Ser A a Phe Asp T ccgacagcct gt t t aaaact cc cgc gt c ier Ar g Val 15 it a ct g aca (al Leu Thr 30 ac ggt at g yr 03 y I'b t Page cat agcggat gggat aagt t at g cat t ca aag gaa I'b t Hi s Se r Lys O31u 1 ct c t cg gt a gcg gt t Leu Ser Val A a Val ggc acc ggc at c gca O3 y Thr 03 y Il e A a gat cca aac atg aac Asp Pr o Asn I'b t Asn seql i st cor r ect ed. t xt tac aac ccg atc gat gac Tyr Asn Pro lie Asp Asp ctt ccc tac ttc gga agt Leu Pro Tyr Phe y Ser 75 acc gcc tca tcc ggc gtc Thr Al a Ser Ser G y Val ccg cgc tac ccc ctc ggc Pro Arg Tyr Pro Leu G y 105 atg gag cca gaa gtt ctt e t G u Pro G u Val Leu 120 ggt gat cgc atc cgc caa Sy Asp Arg lie Arg n 135 acc att cct cta gtc tgg Thr lie Pro Leu Val Trp 150 155 cca acg gtc tac gca ctc Pro Thr Val Tyr Ala Leu 170 aac tgg gtc acc cgc acc Asn Trp Val Thr Arg Thr 185 atc aac ctc atc atg ccg Ile Asn Leu Ile IVet Pro 200 tgg gca cgc gaa agc caa Trp Ala Arg G u Ser G n 215 ttg ctc atg cac gaa ctg Leu Leu IVet His G u Leu 230 235 <210> 44 <211> 242 <212> PRT <213> Corynebacter i um gl <400> 44 Met His Ser Lys G u G u 1 5 Leu Ser Val Ala Val Ala G y Thr G y I e Al a Al a Asp Pro Asn Iet Asn Tyr at c I e 60 aaa Lys gt g Val aaa Lys gcg Al a at c SIle 140 gtt Val gga Gy gac Asp at g Met t cc Ser 220 cct Pr o gat Asp acc Thr t cc Ser gac Asp 110 ctt Leu gcg Al a cca Pr o ggt Gy gag G u 190 gga Gy ggt Gy ccg Pr o ccc Pr o tgg Tr p ct c Leu ccc Pr o cga Ar g tcg Ser gac Asp 160 ggt Gy tta Leu ttt Phe gcg Al a gt a Val 240 gaa G u ggc Gy ccg Pr o aag Lys ttc Phe cca Pr o 145 aac Asn gga Gy acc Thr agt Ser caa G n 225 gcg Al a gga Gy t ca Ser cag G n gca Al a gt c Val 130 t ca Ser acc Thr caa G n agt Ser ttc Phe 210 cag G n gcc Al a ttg Leu t ca Ser t ac Tyr acc Thr 115 ggc Sy at g Met gt g Val ggc G y gac Asp 195 t ac Tyr tgg Tr p t cc Ser t at Tyr acc Thr 100 at c SIe gtt Val gga Sy cct Pr o ggc G y 180 aac Asn tct Ser gaa G u ut ari cum Leu Thr Val Arg Lys y lie Ser Arg Val 10 Ser Ser Ile y Phe y Thr Val Leu Thr 25 Al a G n Asp Ser Al a Phe Asp Tyr G y IVet 40 Asn Pro lie Asp Asp lie Lys Asp Arg Pro 55 Page 61 seqi i st car r ect ed. t xt O31u G31y Leu Ser Asn Leu Pro Tyr Phe 03 y Ser Lys Leu Thr Ser G31y Pr o Ly s Ph e Pr o 145 As n G31y Th r Ser O31n 225 A a Ser Ty r Th r 115 G31y vb t ValI G31y As p 1 95 Ty r Tr p A a As p As p As p Ar g G31y 1 65 O31n As n As p Th r A a Ar g O31u As p 135 li1e Th r Tr p As n A a 215 Le u Ser Pr o 1 05 Ou le Le u Ty r Th r 1 85 li1e O31u Hi s G31y 90 Le u ValI Ar g ValI A a 1 70 Ar g vb t Ser (31u ValI G31y Le u O31n Tr p 1 Le u Th r Pr a O31n Le u 235 Th r As p Ar g 125 As n ValI G31y Le u Le u 205 vb t O31u Ser As p 110 Le u A a Pr a G31y O31u 1 (31y G31y Pr a A a Le u O31u Ty r O31u As p 1 O31u Ser cys Le u <210O> <211 <21 2> <21 3> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> 653 DNA Cor ynebact er i urn gI ut ani cumn CDS (630) RXN03 039 VARI ANT 141 Xaa Leu VARI ANT 142 Xaa Pro VARI ANT 166, 180 Xaa Ser VARI ANT 1 78 Xaa Tyr Page 62 seql i st cor r ect ed. t xt <220> <221> VARIANT <222> 195 <223> Xaa G y <400> gca ctc ccg caa Ala Leu Pro G n 1 ctg ccc aaa gca Leu Pro Lys Ala gag cga ttc gtc G u Arg Phe Val tac tcg cca tca Tyr Ser Pro Ser gaa gac aac acc G u Asp Asn Thr gac ggt ggc caa Asp G y y n gag ttg acc agt G u Leu Thr Ser 100 tct ttt agt ttc Ser Phe Ser Phe 115 ggt gcg caa cag Sy Ala G n n 130 cta gaa gcg gcc Leu G u Ala Al a 145 tcc atg tcc ggg Ser lMt Ser y ttt tay tcc tck Phe Xaa Ser Xaa 180 tgg atg ggr cgc Trp IVet Xaa Arg 195 tac acc gac Tyr Thr Asp cca cgc tac Pro Arg Tyr 10 ccc ctc ggc aaa Pro Leu y Lys gac gac Asp Asp acc Thr ggc Sy at g Met gt g Val ggc Sy gaa G u t ac Tyr tgg Tr p at c l Ie gga Sy 165 gt c Val cgn Ar g at c SIle gtt Val gga Sy cct Pr o 70 ggc G y aac Asn gct Al a gaa G u ggc G y 150 tcr Xaa ggc Sy tgg Tr p gac Asp gac Asp cgc Ar g 55 ggc Sy caa G n aac Asn gac Asp aca Thr 135 gca Al a gt g Val t ca Ser cat Hi s gag G u 25 gat Asp at t SIle acg Thr tgg Tr p aac Asn 105 gca Al a ct c Leu ggg G y aac Asn tct Ser 185 agc Ser cca Pr o cgc Ar g cct Pr o gt c Val gt c Val 90 ct c Leu ggc G y at g Met caa G n ttt Phe 170 gga Sy cac Hi s gaa G u at c SIle ct a Leu t ac Tyr 75 acc Thr at c SIle gaa G u cac Hi s cgc Ar g 155 gcg Al a tgt Cys tgc Cys gct Al a cgc Ar g gt c Val gca Al a cgc Ar g at g Met agc Ser gaa G u 140 agc Ser acg Thr gcc Al a ct a Leu ctt Leu caa G n tgg Tr p ct c Leu acc Thr ccc Pr o gaa G u 125 ct r Xaa at c SIle cat Hi s gaa G u caa G n 205 gcg Al a at c SIle gt c Val ggc Sy gac Asp at g Met 110 t cc Ser ccm Xaa gt c Val gac Asp acc Thr 190 cgg Ar g cgc Ar g aac Asn gt g Val ggc Sy ctt Leu ct c Leu at g let gag G u ggc G y ccc Pr o 175 aac Asn caa G n ctt Leu gcg Al a cca Pr o ggc Sy gat Asp gga G y ggt G y ccg Pr o at g let 160 aac Asn t cc Ser tgt Cys cgt gcc tgagcaaatc tttggtgaag tag Arg Ala 210 <210> 46 <211> 210 <212> PRT <213> Cor ynebact eri um gl ut am cum Page 63 IND 220>seqI i st car r ect ed. t xt <221 VARI ANT <222> 141 <223> Xaa Leu <220> S<221> VARIANT <222> 142 <223> Xaa Pro <220> <221> VARIANT <222> 166, 180 S<223> Xaa Ser 00 S<220> S<221> VARI ANT C1 <222> 178 .C <223> Xaa Tyr S<220> Cl<221> VARI ANT <222> 195 <223> Xaa 03 y <400> 46 Al a Leu Pr o O31n Tyr Thr Asp Pr o Ar g Tyr Pr o Leu G31y Lys Asp Asp 1 5 10 Leu Pro Lys Ala Thr I Ie As p 'b t (31u Pro Ou Ala Leu Ala Arg Leu 25 31u Ar g Phe Val 31y Val As p 1y Asp Arg IleArg On IleAsn Ala 40 Tyr Ser Pr o Ser I'b t G31y Ar g Thr Ilie Pr o Leu Val Tr p Val Val Pr o 55 (31u Asp Asn Thr Val Pr o G31y Pr o Thr Val Tyr Al a Leu G31y G31y G31y 70 75 Asp(1y (1y (1n (1y (1y (1n Asn Trp Val Thr Ar gThr Asp Leu Asp 90 O31u Leu Thr Ser O31u Asn Asn Ilie Asn Leu Ilie I'b t Pr o I'b t Leu G31y 100 105 110 Ser Phe Ser Phe Tyr Al a Asp Tr p Al a G31y O31u Ser O31u Ser Nib t G31y 115 120 125 G31y Al a O31n O31n Tr p O31u Thr Phe Leu I'b t Hi s O31u Xaa Xaa O31u Pr o 130 135 140 Leu(1u Al aAl a Ile (1y Al aAsp (1y (1n Arg Ser Ile Val G31y Nib t 145 150 155 160 Ser I'b t Ser G31y G31y Xaa Val Leu Asn Phe Al a Thr Hi s Asp Pr o Asn 165 170 175 Phe Xaa Ser Xaa Val G31y Ser Phe Ser G31y Cys Al a O31u Thr Asn Ser 180 185 190 Tr p I'bt Xaa Ar g Ar g Tr p Hi s Ar g Ser Hi s Cys Leu O31n Ar g O31n Cys 195 200 205 Ar g Al a 210 Page 64 seql i st cor r ect ed. t xt <210> <211> <212> <213> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> 47 43 DN Co 2 A rynebact eri um gl ut am cum CDS (409) RXN03040 i sc feature 25, 108, 395 n a, t, c, or g VARI ANT 3 Xaa Phe, Ser, Tyr, VARI ANT 99 Xaa Phe, Leu, I e, or Cys or Val <400> 47 at t actctcg ct at aacgat ccttntgctc aacgctgcga agctcgaaga acaagacaac ctctacatct tcgccggt t c cggtgtgt t c tctgaactag at g Met 1 t ca t nc ggt Ser Xaa G y aac gca ccg att Asn Ala Pro lle gat Asp gag gat gcg ttc G u Asp Ala Phe aaa Lys 15 aac cgc gtc ttg Asn Arg Val Leu gtt ggg Val G y ttt gaa at c Phe G u II e gcg acc gat Al a Thr Asp gct atg tcc aac Ala IVet Ser Asn acc tgc acc cat aac ctc aag gct Thr Cys Thr His Asn Leu Lys Ala caa atg ggc atc G n Iet Gy Ile aac atc aac tac Asn Ile Asn Tyr gat Asp ttc cga cca Phe Ar g Pro acc gga Thr G y acc cac gcc tgg Thr His Ala Trp gat Asp 60 tac tgg aac gaa Tyr Trp Asn G u gcg ctc cac cgc ttc Ala Leu His Arg Phe ggt ggt ccc atc ccg Gy Gy Pro lie Pro ttc Phe ccg ttg atg atg Pro Leu IVet IVet ggc ttc ggc ct c G y Phe G y Leu gac Asp atc tat aac cct Ile Tyr Asn Pro aac Asn ggt gtg acc tcc G y Val Thr Ser gag tct tct nt c G u Ser Ser Xaa aga act Arg Thr 100 gtc ttc Val Phe tgatgtgagc cttggcaccn gtg <210> 48 <211> 103 <212> PRT <213> Corynebacter i um gl utam cum <220> Page <221> VARIANT <222> 3 <223> Xaa Phe, <220> <221> VARIANT <222> 99 <223> Xaa Phe, <400> 48 I'bt Ser Xaa G31y Ar g Val Leu Val Hi s Asn Leu Lys Tyr Asp Phe Arg Al a Leu Hi s Ar g G y G y Pro I Ie Ser Ser Xaa Arg 1 00 seqI i st car r ect ed. t xt Ser Tyr or Cys Leu, I I e, or Val Asp Asn Al a Pro IleAsp 0 u Asp Al 10 G31y Phe G31u I Ie G31u Al a I'bt Ser As Al a Al a Th r Asp O1n I'bt Gy Ilie As 40 4 Pr o Thr G31y Thr His Ala Trp Asp Ty 55 Phe Phe Pr o Leu 'b t 'b t O31n G31y P h 70 Pr o I Ie Ty r As n Pro Asn Gy Val Th 90 Thr Val Phe a n p e Cys I Ie As n Le u Ser <210O> 49 <21 1> 835 <212> DNA <213> Cor ynebact er i urn gI ut anm cumn <220> <221 CIDS <222> (835) <223> RXN03051 <400> 49 acat ccagaa gt agt cgt t g agt at cacga gcaagt caac ggaact cccg ct gcct aagc gggacat agt t gcaggggac at c I Ie gt t ValI ggg G31y ct t Le u ccg Pr o tt a Le u g aa G31u gag G31u ac c Thr t ca Ser gag G31u cga Ar g at g vb t cgc Ar g gag (31u gt a ValI aac As n cca Pr o gt t ValI ac c Thr ag c Ser gag G31u ac g Thr 45 gga G31y aat As n aag Lys5 ggt G31y ggc G31y ggt G31y ccc Pr o gag aaa (3 u Lys 15 gca cga Al a Ar g t tc t ca Phe Ser cag tt g 03 n Leu gac ggt As p (1y 80 Page 66 gat agt aaag at aat gt cga at g cgt t ca gat gt t I'b t Ar g Ser As p Val 1 gct gac cag ct a gaa Al a As p 03 n Leu 03 u ct g at g gca acc acc Leu I'b t Al a Thr Thr gt t t gt ggc cga at t Val Cys G31y Arg Ilie t ca t gg ct t ggg cca Ser Tr p Leu G31y Pr o gt t ggc aaa aga agt Val (3y Lys Ar g Ser seql i st cor r ect ed. t xt gaa ttt ttt ggt gga gcc atc tat tgg cac cca G u Phe Phe y G y Al a lie Tyr Trp His Pro 95 gca gtg acc ttg gac ggt ttg cga cag tgg ggg Ala Val Thr Leu Asp G y Leu Arg G n Trp y 105 110 tca ggg cca ttg ggg tac cca acc tct ggt ccg Ser Gy Pro Leu Gy Tyr Pro Thr Ser y Pro 120 125 ccc ctt act cag cga cag act ttt caa ggt ggt Pro Leu Thr G n Arg G n Thr Phe G n y y 135 140 cca ttg act ggc ggt gct gtg tgg ggc gat att Pro Leu Thr Gy Gy Ala Val Trp y Asp Ile 150 155 160 gaa ctt ggc ggc tcg aat cat gcc att ggc atc G u Leu G y G y Ser Asn His Ala I e G y lI e 170 175 cta cct agc ggt act gag tat ttt tac aat aat Leu Pro Ser y Thr G u Tyr Phe Tyr Asn Asn 185 190 att tcg tgg cga aat gat cgt cag aca cgg ttt Ile Ser Trp Arg Asn Asp Arg G n Thr Arg Phe 200 205 cag cgg gtg tgg gat gcg ttg ggt cgg gag acg G n Arg Val Trp Asp Al a Leu y Arg G u Thr 215 220 cct gaa gca gat gaa aca cct gag gtt tct ggt Pro G u Ala Asp G u Thr Pro G u Val Ser y 230 235 240 <210> <211> 245 <212> PRT <213> Corynebacter i um gl ut ari cum <400> Iet Arg Ser Asp Val I e u Leu Pro u y 1 5 10 Ala Asp G n Leu G u Val Ala u Ala Arg Leu Leu Met Ala Thr Thr G y Cys G u Val Met Trp 40 Val Cys y Arg lie Leu Asp Thr Tyr Arg G n Ser Trp Leu y Pro Pro Lys Ser Asn G u Leu 70 Val y Lys Arg Ser G u Phe Phe y y Al a 90 Asp Thr G y Al a Tyr Al a Val Thr Leu Asp G y 100 105 Page 67 gac Asp acc Thr at g Met gac Asp 145 aaa Lys ccg Pr o ttc Phe at g Met ggt G5y 225 aca ggc Thr G y ttg aac Leu Asn 115 gat aca Asp Thr 130 aac t ac Asn Tyr cag cgc G n Arg at c act Ile Thr t cc aat Ser Asn 195 tat ttg Tyr Leu 210 cgt tta Arg Leu gct Al a 100 tgg Tr p aac Asn t ac Tyr t ac Tyr aat Asn 180 gga G y gct Al a ggt G y t at Tyr gaa G u t at Tyr aac Asn gaa G u 165 gag G u aca Thr acg Thr ttt Phe gcg Al a 245 cta ttc cat gtg Leu Phe His Val Ser G u Thr Sy Asn Tyr Ar g G u Al a Phe G n Asp Hi s Tr p Thr bet Asp 145 Lys Pr o Phe Mbet Sy 225 Leu Asn 115 Thr Tyr Ar g Thr Asn 195 Leu Leu Hi s G u Tyr Asn G u 165 G u Thr Thr Phe Al a 245 Sy Leu 135 Leu Leu Pr o Ser Ar g 215 G u seql i st cor r ect ed. t xt Pro Leu y Tyr Pro Thr 120 125 Thr G n Arg G n Thr Phe 140 Thr Gy Gy Ala Val Trp 155 G y G y Ser Asn Hi s Al a 170 Ser y Thr G u Tyr Phe 185 Trp Arg Asn Asp Arg G n 200 205 Val Trp Asp Ala Leu y 220 Ala Asp G u Thr Pro G u 235 Gy Gy Asp Gy 175 Asn Ar g G u Ser <210> 51 <211> 1704 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1681) <223> RXN03054 <400> 51 ggtggaaata cgcgcacaac aattttattc acagaactta cagtttgttc acatcaacta gtaacgaaag gatcatgtga aag Lys gca Al a caa G n gt g Val cac Hi s ct c Leu gca Al a gt g Val gcg Al a gac Asp gct Al a ccg Pr o gt c Val cag G n gtt Val gt g Val cga Ar g gag G u at t SIle ggg Gy caa G n ggt Sy caa G n 75 at g Met gct Al a caa G n cag G n ggc Sy gcc Al a tgg Tr p gca Al a gct Al a ttc Phe 45 t ac Tyr caa G n t ca Ser gca Al a agt Ser cac Hi s act Thr gaa G u gat Asp ctt ctt Leu Leu 15 cag gtg G n Val cac gaa Hi s G u gag caa G u G n tct ggc Ser G y 80 aag gtg Lys Val Page 68 tgattttttc gggttagggt atg aaa ctg ttt tcc IVet Lys Leu Phe Ser 1 gtt gca ggt ggt ata Val Ala G y y lle gtc aca cct gaa gac Val Thr Pro G u Asp ggg aat acc cca cct Sy Asn Thr Pro Pro gaa atc gcc gag atc G u Ile Ala G u Ile gca cct aat gaa gag Ala Pro Asn u u gag ctg cca gta act G u Leu Pro Val Thr seql i st cor r ect ed. t xt gag ata gct att G u Il e Ala I e att gat aaa II e Asp Lys caa t ct cag G n Ser G n 120 gcc gct gat gag Ala Al a Asp G u gca Al a 110 100 gca cag caa Al a n G n 115 gcg tgt cag Ala Cys G n cca cag acg cga Pro G n Thr Arg ctt gct gcg gct Leu Al a Al a Al a acg ttt Thr Phe 135 t gg ccg t ca cct Trp Pro Ser Pro cat Hi s 140 cag gtt tgt ggt Sn Val Cys y gct Al a 145 att tta gag cgc lie Leu u Arg t at Tyr 150 att cag cag ggt lie Gn G n y gcc Al a 155 cag ttt ggg tgg G n Phe y Trp at g Met 160 ttg ttt ccg agt Leu Phe Pro Ser gaa G u 165 ggc caa acg tt a Sy G n Thr Leu aat Asn 170 cct gat ggt cag Pro Asp G y G n ggg tat cgt cag cgg ttt atg Sy Tyr Arg G n Arg Phe IVet 175 180 aat ggg ttt Asn G y Phe aat t ac agt Asn Tyr Ser 200 tat tgg cat ccg Tyr Trp His Pro aca Thr 190 act ggt gcg cat Thr G y Al a Hi s gct gtt aat Al a Val Asn 195 tct ggg tgg Ser G y Trp gcg cag gtg tgg Ala G n Val Trp cgt aat ggg tgg Arg Asn y Trp gag 10u 210 atg ggt MVet y 215 tat ccc act ggt Tyr Pro Thr G y ggt G0y 220 gaa gtc cct gtg G u Val Pro Val ggt t cc aat ccg Sy Ser Asn Pro at t SIle 230 gat ggt gag ttg Asp G y G u Leu ggg tgg gtg caa Sy Trp Val n act Thr 240 ttc caa ggt ggg Phe n G y G y gtg tat cgc agt Val Tyr Arg Ser ccg Pr o 250 gta ttg gac ggt Val Leu Asp y cag gtg gcc agt G n Val Ala Ser att aat Ile Asn 260 ggg ct g at c G y Leu Il e ctt ggt ttt Leu G y Phe 280 gat aaa tgg ctt Asp Lys Trp Leu gaa G0u 270 ttg ggt ggt cct Leu G y G y Pr o gat agt gac Asp Ser Asp 275 ggt gtg ggt Sy Val y ccc att gcg gat Pro lie Ala Asp gct gtg aca gct Ala Val Thr Ala gac Asp 290 aga ttt Arg Phe 295 tct gtt ttc cag Ser Val Phe G n aac Asn 300 gga gtt gtc tac G y Val Val Tyr cat ccg caa cac His Pro G n His gga Sy 310 gct cac cct at a Ala His Pro Ile tta Leu 315 ggg aat ata tac G y Asn I e Tyr agt Ser 320 atc tgg aga gaa lie Trp Arg u gga gct gag agt G y Al a u Ser ggg Sy 330 gaa ttc ggt tac G u Phe G y Tyr atc ggc gat cca Ile y Asp Pro gaa aag G u Lys 340 1027 1075 1123 1171 1219 tat aca gaa aac atg gct aat cag gta ttc gaa aaa ggc gaa ctt gca Tyr Thr G u Asn IVet Al a Asn G n Val Phe G u Lys G y G u Leu Al a 345 350 355 gct aac cta tac ccc aat cct ctt gag Ala Asn Leu Tyr Pro Asn Pro Leu G u gct ttt att gag ttt tta ccc Ala Phe Ile G u Phe Leu Pro Page 69 seql i st cor r ect ed. t xt 5 370 360 ttt gct aat ctt gag gaa gca ata gag tat Phe Ala Asn Leu G u G u Ala lie G u Tyr 375 380 aat tct cgt gta gag gcg aat tca ctt aac Asn Ser Arg Val G u Al a Asn Ser Leu Asn 390 395 caa tgt caa tcg caa tcc gct aac att cat G n Cys G n Ser G n Ser Ala Asn Ile Hi s 410 415 gga gtc ggg att agg gtt cca aag att ggg Sy Val y Ile Arg Val Pro Lys Ile y 425 430 tgc gac ctt cct gga act gtc tca gat gta Cys Asp Leu Pro y Thr Val Ser Asp Val 440 445 tac tac gac tat tgg gga cga tgg gct caa Tyr Tyr Asp Tyr Trp G y Arg Trp Ala G n 455 460 ttc ttc ggt aat agg aat tct gtt gtg caa Phe Phe y Asn Arg Asn Ser Val Val G n 470 475 tgc agc ggg gag aag aat aca tta ttt tgg Cys Ser y G u Lys Asn Thr Leu Phe Trp 490 495 gtg act tat gaa ggt cag ccg tat ttc ggt Val Thr Tyr G u y G n Pro Tyr Phe y 505 510 gct tat ctt ccg tgt acg ata gac cgt agt Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser 520 525 gag <210> 52 <211> 527 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 52 NIet Lys Leu Phe Ser Lys Al a Al a G y Val 1 5 10 Val Ala G y Gy Ile Ala Pro Val Ala n 25 Val Thr Pro G u Asp G n Asp Ala Tyr Val 40 Sy Asn Thr Pro Pro Val Val Asp y Val 55 G u Ile Ala G u Ile His G u Ala lie Arg 70 Ala Pro Asn G u G u Leu lie Pro G y u 90 Pag ttt gag Phe G u 385 gcc aag Al a Lys 400 gtg aga Val Arg ttt aag Phe Lys gtg ggg Val G y gca gca Al a Al a 465 acc aat Thr Asn 480 ggt act G y Thr cag t ca G n Ser aac Asn aaa Lys acg Thr gct Al a t at Tyr 450 t at Tyr tta Leu t ca Ser gca Al a gga G y gat Asp aag Lys agg Ar g 435 gga Sy gca Al a gag G u t at Tyr act ttg Leu tcg Ser agt Ser 420 at g Met tgg Tr p caa G n gcg Al a ttt Phe 500 aat t ca Ser at t SIe 405 gac Asp gat Asp at t SIe caa G n ggt G y 485 cag G n t ac 1267 1315 1363 1411 1459 1507 1555 1603 1651 1701 1704 Thr Asn Tyr taacataagg aatggaatag SIle Sy G n Sy G n M\et e Al a G n G n Sy Al a Tr p Al a Al a Phe Tyr G n Ser Al a Ser Hi s Thr a u Asp Leu G n Hi s G u Ser Lys seql i st cor r ect ed. t xt Su Leu Pro Val Thr Ile Asp Lys Ala Ala Ala Asp Gu Ala Ou lie 100 Al a Al a Al a 145 Leu Ar g Al a Tr p Asn 225 Phe Val Gy Al a Tr p 305 I le Gy Lys SIle G u 385 Lys Ar g Lys Al a 115 Al a Leu Pr o Ar g Al a 195 Ser Ser Gy Ser Asp 275 Gy Pr o Ar g Pr o G u 355 Phe Gy Asp Lys Ar g 435 G n G n Ar g G u 165 vbt Asn Tr p Pr o Ar g 245 Asn Asp Sy Hi s G u 325 Lys Al a Pr o Ser SIle 405 Asp Asp Ser Phe 135 I le G n Sy Tyr Sy 215 Asp Tyr Leu Sy Phe 295 Al a Al a Thr Asn Al a 375 Ser Cys Val Asp 105 Pr o Pr o G n Leu Val 185 Al a Pr o G u Ser Leu 265 Pr o Val Pr o Ser Asn 345 Tyr Leu Val Ser SIle 425 Pr o Thr Pr o Al a 155 Pr o Tr p Val Sy Ser 235 Val Lys Al a G n Leu 315 G u Al a Asn G u Al a 395 Ser Val Thr Ar g Hi s 140 G n Asp Hi s Tr p Sy 220 Gy Leu Tr p Asp Asn 300 Sy Phe Asn Pr o Al a 380 Asn Al a Pr o Val Sy 125 G n Phe Sy Pr o G u 205 G u Tr p Asp Leu G u 285 Sy Asn Sy G n Leu 365 l le Ser Asn Lys Ser 445 110 Leu Al a Al a Val Cys G y G y Trp Met 160 Sn G y Tyr 175 Thr Thr G y 190 Arg Asn G y Val Pro Val Val G n Thr 240 G y Phe G n 255 G u Leu G y 270 Al a Val Thr Val Val Tyr I e Tyr Ser 320 Tyr Pro Il e 335 Val Phe G u 350 G u Al a Phe G u Tyr Phe Leu Asn Al a 400 Ile His Val 415 ile G y Phe 430 Asp Val Val Sy Tyr y Trp lie Tyr Tyr Asp Tyr Trp y Arg Trp Ala G n Ala Page 71 seqI i st car r ect ed. t xt 450 455 460 Al a Ty r Al a O31n O31n Ph e Phe Gy Asn Arg Asn Ser Val Val O31n Th r 465 470 475 480 Asn Leu G31u Al a G31y Cys Ser G31y G31u Lys Asn Thr Leu Phe Tr p (31y 485 490 495 Thr Ser Tyr Phe O31n Val Thr Tyr O31u O31y O31n Pr o Tyr Phe O31y O31n 500 505 510 Ser Al a Thr Asn Tyr Al a Tyr Leu Pr o Cys Thr Ilie Asp Ar g Ser 515 520 525 <210O> 53 <21 1> 456 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221 CIDS <222> (433) <223> RXN02949 <400> 53 act ct cgaag gt t gaacaca gggct gcgat t gt gct ggat t gt tat cgcc cct ggat gag t agt gat t ta gaggagt gct aat As n t cg Ser t ct Ser gt t ValI t gg Tr p tt c Ph e ggc O31y gct Al a c ag O31n t ct Ser act Thr at t I Ie ggc O31y act Thr aag Ly s ct g Le u cgc Ar g tt g Le u gga O31y acc Thr t ca Ser cct Pr o cag O31n 75 acc Thr ac g Thr t ct Ser t cc Ser gag O31u at g vb t gct Al a cgc Ar g t ct Ser t cg Ser gt g ValI ac g Thr gt g ValI cca Pr o 15 t ct Ser g at As p gga O31y t ac Tyr t ct Ser 95 ac g Thr t at Tyr t ct Ser g aa O31u ac c Th r 80 ggt O31y caaat gt ct g cacgaaaaat gt g agc gac gag cag Val Ser As p O31u O31n 1 ggt aaa cgc cag ct g O31y Lys Ar g O31n Leu gag gct aag cag gt a O3 u Al a Lys 03 n Val aag cct ggc ggc ggt Lys Pr o O31y O31y O31y gt c cgt aag gt t at t Val Ar g Lys Val Ilie ct t gt c gt t t tg gga Leu Val Val Leu 03 y gt g gat t tc ct a gct Val Asp Phe Leu Al a 1 00 ggt ct t gga gt t O3 y Leu 03 y Val 1 05 tt t gag aag at t ct g 0 u Lys II e Leu act ccg Thr Pr o 110 t aggt aggat gt gt aacat c <210O> 54 <211> 111 <21 2> PRT <21 3> Cor ynebact er i urn gl ut anm cumn Page 72 <400> 54 Val Ser Asp O31y Lys Ar g (3 u A a Lys Lys Pr o O31y Val Ar g Lys Leu Val Val Val Asp Phe O31n Le u ValI G31y I Ie G31y A a Ser G31y Thr I Ie 55 Pr o Le u Le u seql i st car r ect ed. t xt G31y Val G31y G31y Thr Ser 10 A a Se r Thr Thr Ser Thr 03 n Lys Lys Ser Ser 03 y 40 Ser Phe Leu Pr o O31u Val Thr Ala Arg O31n l'b t Val li1e Val Leu Thr Ala Leu 90 G31y Val O31u Lys I Ie Leu 1 Pr o Ser As p G31y Ty r Ser Pr o <210O> <21 1> 1941 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1918) <223> RXN02462 <400> t ccat cct ca t cgacgaagc ccgcacccca ct gat tat ct at cgcagt t c t acaacgt ct t cgcacagat cgt cccacgc ccgggaccag t agacggcac c ac Hi s ggC G31y cct Pr o c ag O31n gt t ValI t ac Tyr at c I Ie g aa (31u g aa (31u c ac Hi s ct g Le u at c I Ie g aa (31u aac As n g ac As p gt c ValI c ag O31n ac c Thr g ac As p at g vb t aac As n gaa O31u gaa O31u ct g Le u cgc Ar g ggC O31y 75 cac Hi s cag O31n cgt Ar g g ac As p gt c ValI g ac As p tt c Ph e c ag O31n ac c Thr aaa Ly s c aa O31n ag c Ser 45 aag Ly s ac c Thr gcg A a ct g Le u aag Ly s ct c Le u t ac Tyr g ac As p ggC O31y at c I Ie gcg A a 110 ac c Thr 15 ggC O31y ct g Le u t ac Tyr cgt Ar g g aa O31u 95 ac c Thr gt c ValI at c I Ie aac As n at c I Ie gt c ValI 80 gcc A a gt t ValI at g vb t 1 ggt O31y g ac As p aac As n gt c ValI ct t Le u aaa Ly s ac c Thr gt g ValI aac As n gcc A a cgc Ar g gcc A a gag O31u ct c Le u aaa Ly s ct c Le u at c I Ie aac As n ggC O31y cgc Ar g C ag O31n 115 g aa O31u t ac Tyr aag Ly s ggC O31y cgc Ar g gt a ValI 1 00 aac As n acc aag gac Thr Lys Asp gt t ValI g aa O31u gca A a gca A a g aa O31u cga Ar g gag O31u t ac Ty r Page 73 ttc cgc Phe Arg gaa gca G u Al a 135 cca acc Pro Thr 150 aaa acc Lys Thr cgc acc Arg Thr cgc t cc Arg Ser aac gt c Asn Val 215 cag gca G n Al a 230 cgt gga Arg G y atc aaa I e Lys tac cag Tyr G n gaa gaa G u G u 295 ggc acc G y Thr 310 cgt tct Arg Ser atg cgc lIet Arg atg at g Mvet Mvet acc gt c Thr Val ctc tac acc aaa ct c Leu Tyr Thr Lys Leu seql i st cor r ect ed. t xt gcc ggc atg acc ggt acc gca gag acc Al a G y lMt Thr G y Thr Al a G u Thr 120 gca Al a aac Asn caa G n gaa G u gaa G u 200 ct c Leu ggt Sy acc Thr ct c Leu gaa G u 280 cgt Ar g gaa G u gca Al a gac Asp aac Asn 360 acc Thr ct c Leu cca Pr o gct Al a 170 ggc Sy ct c Leu gcg Al a cca Pr o at c SIle 250 gaa G u tgg Tr p gac Asp cac Hi s cag G n 330 ct g Leu ct c Leu t cc Ser aac Asn aac Asn 155 aag Lys caa G n t cc Ser aag Lys ggc Sy 235 gt g Val cgt Ar g gac Asp aaa Lys gaa G u 315 ggc G y at g bet aac Asn at c SIle cag G n 140 cag G n ttc Phe cca Pr o cag G n cac Hi s 220 gcc Al a ct c Leu gga Sy gct Al a gt c Val 300 t cc Ser gac Asp gtt Val gt c Val aag Lys 380 at c SIle cgc Ar g gca Al a gt c Val ct g Leu 205 cac Hi s gt c Val ggc Sy ctt Leu gaa G u 285 cgc Ar g cga Ar g cca Pr o cgc Ar g cca Pr o 365 ggc G y t ac Ty r gaa G u gca Al a ct c Leu 190 ttg Leu gag Gu acc Thr gga Sy gat Asp 270 ctt Leu gaa G u cgc Ar g gga G y ttc Phe 350 gac Asp gcc Al a aag Lys gac Asp gt c Val 175 gt c Val acc Thr cag G n gtt Val aac Asn 255 cct Pr o cca Pr o gcc Al a at c SIle t cc Ser 335 gt c Val gat Asp caa G n ct c Leu ttg Leu 160 gt c Val ggt G y aaa Lys gaa Gu gcc Al a 240 cca Pr o ttc Phe gca Al a gga Sy gac Asp 320 acc Thr ggc G y gt g Val gct Al a gac Asp 145 acc Thr gac Asp acc Thr cga Ar g gca Al a 225 acc Thr gaa G u gaa G u at g Met gga Sy 305 aac Asn cgc Ar g cca Pr o ccc Pr o cag G n 385 130 gt c Val gac Asp gac Asp gt c Val ggc G y 210 cag G n aac Asn at c SIle gac Asp aag Lys 290 ct c Leu cag G n ttc Phe acc Thr at c SIle 370 gt g Val at c SIle ttg Leu at c SIle t cc Ser 195 at c SIle at c SIle at g let ct c Leu gaa G u 275 cag G n t ac Tyr ct g Leu t at Tyr at g Met 355 gaa agu gag G u gcg Al a gt g Val gca Al a 180 gt c Val aag Lys gtt Val gcg Al a ct c Leu 260 gaa G u cga Ar g gt c Val cgc Ar g ct c Leu 340 gaa G u t cc Ser aac Asn at c SIe t ac Tyr 165 gaa G u gag G u cac Hi s gct Al a ggc Sy 245 gac Asp agc Ser tgc Cys ctt Leu ggt G y 325 t ct Ser aac Asn aaa Lys cag G n 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Page 74 aac As n 390 g aa G31u gca A a gca A a ct c Le u aac As n 470 ct g Le u g aa G31u C ag O31n aaa Ly s ggc G31y 550 g aa G31u ac C Thr cgg Ar g at g t tc gaa at g Phe 03 u [Vbt S cgt aag aac gt t Arg Lys Asn Val 395 eqi i st car r ect ed. t xt ct g aag t ac gac ga Leu Lys Tyr 400 As p 3 a gt c at g aac u Va Ilb ~t As n cgc Ar g at c I Ie gt c ValI 440 aaa Ly s ac c Th r gcc A a gca A a cgc Ar g 520 cgc Ar g cgc Ar g ggc G31y cgc Ar g t cg Ser 600 gt t ValI 410 cgc Ar g ggc G31y t gg Tr p ct c Le u g at As p 490 ct c Le u at c I Ie c ac Hi s at g vb t at g vb t 570 ct c Le u at c I Ie t ac Ty r gcc A a aac As n gt c ValI 475 ct a Le u gaa G31u gaa G31u ct c Le u gca A a 555 t t c Ph e t t c Ph e t ac Ty r at c I Ie ac c Th r gcc A a 460 g aa G31u cgc Ar g g aa G31u cga Ar g t ac Ty r 540 c ag O31n aac As n ct c Le u ag c Ser c aa O31n gcc A a 445 ct c Le u ggc G31y ac c Th r gcc A a at g vb t 525 g aa O31u cgc Ar g ggc G31y t cc Ser gag (31u aac As n 430 aac As n g aa O31u ag c Ser gca A a gt a ValI 510 gt g ValI at g vb t g ac As p at g vb t gca A a 590 cga Ar g 415 at g G31y gcc A a g aa O31u ct c Le u 495 t cc Ser ct c Le u g ac As p cca Pr o aaa Ly s 575 ag c Ser cgc Ar g at c I Ie t ac Ty r ct c Le u t ac Ty r 480 gt c ValI gca A a at g vb t t ac Ty r ct g Le u 560 g ac As p agt Ser g aa (31u g aa O31u gt c ValI t ac Ty r 465 ggc G31y aac As n at c I Ie cca Pr o ct g Le u 545 gt c ValI G31y t ca Ser at c I Ie g aa O31u g aa O31u 450 g ac As p aaa Ly s g ac As p ggc G31y gt c ValI 530 aaa Ly s g aa O31u at c I Ie t ca Ser ct c Le u ac a Th r 435 g ac As p cca Pr o cca Pr o gcc A a ggc G31y 515 at c I Ie g aa O31u t ac Ty r aag Ly s ag c Ser 595 g aa (31u 420 gt c ValI t gg Tr p t cg Ser ggg G31y c ac Hi s 500 g aa O31u g ac As p ggc G31y c aa O31n g aa O31u 580 aag Ly s 405 t cc Ser ag c Ser g ac As p at c I Ie gag O31u 485 gcc A a gca A a ac c Th r at c I Ie aag Ly s 565 g aa O31u ac g Th r 1315 1363 1411 1459 1 507 1 555 1 603 1651 1 699 1 747 1 795 1 843 1891 1 938 1941 ct g act aac t ca Leu Thr Asn Ser gaa ccc O31u Pro 605 t gaaat t cag cat ccgccac <210O> 56 <21 1> 606 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 56 l'bt Thr Lys Asp Val Hi s Tyr O31u Val Asp O31u Ar g Lys Lys Thr Val 1 5 10 Page seqi i st car r ect ed. t xt OyVai Lys Ou OuOyVai OuTyr Vali(uAsp(3n 25 Leu 03 y I I e As p As n ValI Le u Ly s Thr O31y As p 145 Thr As p Thr Ar g Al a 225 Thr O31u O31u vb t O31y 305 As n Ar g Pr o Le u li1e As n O31y Ar g O31n 115 Al a I Ie Le u I Ie Ser 1 95 le le vb t Le u O31u 275 O31n Tyr Le u Tyr vb t 355 Al a Al a O31u Ar g O31u Tyr Thr I Ie Tyr 1 65 O31u O31u Hi s Al a O31y 245 As p Ser cys Le u O31y 325 Ser As n O31u O31u vb t As n Ly s Ar g Al a 135 Thr Thr Thr Ser ValI 215 Al a O31y Ly s O31n O31u 295 Thr Ser Ar g vb t Hi s 40 Le u I Ie O31u As n Le u 120 Al a As n O31n O31u O31u 200 Le u O31y Thr Le u O31u 280 Ar g O31u Al a As p As n 360 Ser Ph e ValI O31y O31u 1 Tyr O31u Ar g O31u Ly s 1 Tyr As n Le u As p Ar g 265 Al a O31y Ar g Ar g As p 345 Ar g O31n Thr As p vb t 90 As n Thr Le u Pr o Al a 1 70 O31y Le u Al a Pr o I Ie 250 O31u Tr p As p Hi s O31n 330 Le u Le u Le u Ar g O31y Hi s O31n Ly s As n As n 1 Ly s O31n Ser Ly s O31y 235 ValI Ar g As p Ly s O31u 315 O31y vb t As n Ser Ly s Thr Al a Le u Al a 125 li1e Ar g Al a ValI Le u 205 Hi s ValI O31y Le u O31u 285 Ar g Ar g Pr o Ar g Pr a 365 Le u Tyr Ar g O31u Thr vb t Ly s As p ValI 1 ValI Thr O31n ValI As n 255 Pr a Pr a Al a I Ie Ser 335 ValI As p Pro lie cuSer Lys Thr Val Thr Asn Ser 370 375 I I e Lys 380 0 y Al a(0 n Al a Page 76 seqI i st car r ect ed. t xt G3 n Val a3 u Asn G3 n Asn Phe G3 u [vbt 385 390 As p a31u a31u ValI Tyr 465 a31y As n I Ie Pr o Le u 545 ValI a31y Ser ValI Le u Thr 435 As p Pr o Pr o Al a G y 515 li1e a u Tyr Ly s Ser 595 As n 405 Ser Ser As p I Ie a31u 485 Al a Al a Thr I Ie Ly s 565 a31u Thr a31u Al a Al a Le u As n 470 Le u a31u G n Ly s a31y 550 a31u Thr Ar g a31n As p Tyr As p 455 Tr p Ser Tyr I Ie Tr p 535 Le u a31y ValI Ly s Ar g I Ie ValI 440 Ly s Thr Al a Al a Ar g 520 Ar g Ar g a31y Ar g Ser 600 Ly s Ser 425 As p Le u As p a31u Ly s 505 As n a31u Al a As p a31n 585 Le u Ar g Lys 395 Val I Ie 410 Ar g Ty r a3 y Al a Tr p As n Leu Val 475 Asp Leu 490 Leu a3 u I Ie G u Hi s Leu [vb t Al a 555 [Vb t Phe 570 Leu Phe Thr As n Asn Val Leu Lys Tyr I Ie Thr Al a 460 a31u Ar g a31u Ar g Tyr 540 a31n As n Le u Ser Ser a31n Al a 445 Le u a31y Thr Al a vb t 525 a31u Ar g a31y Ser a31u 605 a31u As n 430 As n a31u Ser Al a ValI 510 ValI vb t As p vb t Al a 590 Pr o Ar g 415 vb t a31y Al a a31u Le u 495 Ser Le u As p Pr o Ly s 575 Ser <210O> 57 <21 1> 1965 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1942) <223> RXN01559 <400> 57 gt ctggt tga t tggaat tga aggagact tt ct tggct cgg caaaaaaaga gtgccgctag cgcct gggaa cgat ggccaa aacgcgcaat agcgt tgt tt gt g ct c at c gt c gt t 115 Val Leu I I e Val Val 1 ggt gt t t at gcg t tg gt g ct g t tg aca ggc gat cgt t ct gcc aca cca 163 ay Val Tyr Ala Leu Val Leu Leu Thr a31y Asp Ar g Ser Al a Thr Pr o 15 aaa t tg ggt at t gat ctg caa ggc gga acc cga gt g acc ct c gt g ccg 211 Lys Leuc3y Ilie Asp L eu (31n C3y C3y Thr Ar g Val Thr Leu Val Pr o 30 Page 77 seql i st cor r ect ed. t xt cag G n ct g Leu gct Al a cag G n ggt Sy gag Su gca Al a gcg Al a 150 gcg Al a cgc Ar g ccc Pr o gag Su gca Al a 230 ttg Leu aat Asn t cc Ser gaa Gu cag G n aac Asn ggt Sy caa G n gca Al a gcc Al a 120 gcc Al a gaa G u cct Pr o gaa G u gt c Val 200 gat Asp gat Asp aac Asn at c SIle cag G n 280 tct Ser cca Pr o gt g Val acg Thr ct a Leu at g let agg Ar g ttg Leu gaa G u gat Asp 170 acg Thr at c SIle ttg Leu gct Al a gaa G u 250 acc Thr gaa G u act Thr act Thr aac Asn ct g Leu 75 gga G y ccc Pr o tgg Tr p gag G u gaa G u 155 gag G u gac Asp gct Al a gcc Al a gt a Val 235 acc Thr aac Asn at c SIle tgg Tr p cag G n ggc G y gt g Val cag G n gat Asp gtt Val gaa G u 140 gca Al a cca Pr o at g Met gca Al a ggc G y 220 ggt G y gat Asp cgt Ar g agc Ser t cc Ser 300 cag ct g aat G n Leu Asn ggc G y act Thr t cc Ser acc Thr 110 t ac Tyr aac Asn gag G u aac Asn cgc Ar g 190 tct Ser gat Asp gt g Val gaa G u at c SIle 270 gcc Al a ct g Leu t ca Ser ccc Pr o ct g Leu ttg Leu gt c Val gct Al a gaa G u 160 at t SIle gac Asp at g Met cca Pr o gt a Val 240 ggt G y ggt G y aaa Lys cag gca G n Al a ggt gca G y Al a ggg gaa G y G u ctg ttc Leu Phe atg cca let Pro atc acc Ile Thr 130 gtt gca Val Ala 145 ccc gt c Pro Val gag gca G u Al a cgc cag Arg G n cag tgc Sn Cys 210 cgc ctg Arg Leu 225 ctt gat Leu Asp gcg cgc Al a Ar g gga ttc G y Phe t cc ggc Ser G y 290 cgc acc Arg Thr agc gtg Ser Val aat acc Asn Thr cgt ccc Arg Pro 100 gag ct g G u Leu 115 gaa gag G u G u t cg acc Ser Thr acc gtg Thr Val aca cag Thr G n 180 t cc acc Ser Thr 195 acc act Thr Thr cca ttg Pro Leu cct gca Pro Al a ct a acc Leu Thr 260 aac gcc Asn Ala 275 gat ggg Asp G y 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 acc agc cag tac ctg cag cag Thr Ser G n Tyr Leu G n G n 305 Page 78 seql i st cor r ect ed. t xt cag G n 310 cag G n act Thr ttg Leu acc Thr at c SIle 390 t ac Tyr ggc Gy t at Tyr acc Thr at c SIle 470 agc Ser gct Al a ttc Phe at c SIle aag Lys 550 gcc Al a gca Al a act Thr ct g Leu 360 gtt Val ggc G y cgc Ar g ttg Leu ct a Leu 440 gcc Al a gaa G u aag Lys gt g Val ct g Leu 520 gca Al a tcg Ser atc acc ctg gac tct cag gtg att lie Thr Leu Asp Ser G n Val lile 315 320 acc cct gt g ggt tct gca aca tcc Thr Pro Val Gy Ser Ala Thr Ser 330 335 gaa gcc caa gat ctg gcg aac aac G u Ala G n Asp Leu Ala Asn Asn 345 350 agc ttc gca ggt gaa aac ggc gag Ser Phe Ala Gy G u Asn Gy u 365 ccg cca tca cta ggc gca gca tcc Pro Pro Ser Leu Gy Ala Ala Ser 380 atc gtc ggc atc gcg ctg gtc gcc Ile Val Gy Ile Ala Leu Val Ala 395 400 gtc ttc gga ttc gtt tcc ctg ttc Val Phe G y Phe Val Ser Leu Phe 410 415 gtc tac ggc ctt ctg gta ctg ctg Val Tyr G y Leu Leu Val Leu Leu 425 430 gac ctt gct ggt atc gcc ggt ttg Asp Leu Ala y Ile Ala y Leu 445 gac tcc ttc gtg gtg ttc tat gag Asp Ser Phe Val Val Phe Tyr G u 460 gga aga tcc ttt aga tct gca gta Sy Arg Ser Phe Arg Ser Ala Val 475 480 cgc acc atc gtc aca ggc aac atg Arg Thr Ile Val Thr Gy Asn IVet 490 495 att tac ttg ctc gcg gtc ggc gaa Ile Tyr Leu Leu Ala Val Gy u 505 510 ggt ctg acc acc gta ttc gat ctc G y Leu Thr Thr Val Phe Asp Leu 525 cca ctg gtt atc ctg gca tca cgc Pro Leu Val lie Leu Ala Ser Arg 540 gtc aac ggc atg gga cga gtg atg Val Asn G y vbt Gy Arg Val Vbet 555 560 aac ggt gaa ttg gat gag cct gag Asn G y G u Leu Asp G u Pro G u 570 575 tct gca ccc Ser Ala Pro at c acc ggt Ile Thr G y ct g cgc t ac Leu Arg Tyr 355 cgc ggc gga Arg G y y 370 ttg aag gcc Leu Lys Al a 385 atc ttc gtg i e Phe Val acc ctg ttt Thr Leu Phe gga cgc tgg G y Arg Tr p 435 at c at c ggt lie l e G y 450 cgc atc aag Arg Ile Lys 465 cct cgt gca Pr o Ar g Al a gtc act ttg Val Thr Leu gtc aag ggc Val Lys G y 515 gtt gtc acc Val Val Thr 530 aac cca ttc Asn Pro Phe 545 aag ctc gtt Lys Leu Val t ac ct g aaa Tyr Leu Lys gt g Val gac Asp 340 ggt G y act Thr gga G y ttc Phe gcc Al a 420 at c SIle at c SIle gat Asp tgg Tr p ct c Leu 500 ttt Phe ttc Phe ttt Phe gaa G u at t SIe 325 ttc Phe gca Al a acc Thr ct g Leu gcc Al a 405 gca Al a gga Gy ggt Gy gag G u gaa G u 485 ggc G y gcc Al a ct g Leu gcc Al a gaa G u 565 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 cgc cgc gcc Arg Arg Ala aag at c Lys II e 580 Page 79 IO seql i st cor r ect ed. t xt cat gcc aag aat gcg gca gct gat aag gct tcc act gac aat tct tcc 1891 SHis Ala Lys Asn Ala Al a Ala Asp Lys Ala Ser Thr Asp Asn Ser Ser 585 590 595 act gac aat tct gaa gca cct ggc acc gat acg aac caa gag gag gag 1939 L Thr Asp Asn Ser G u Ala Pro G y Thr Asp Thr Asn Gn G u G u G u 600 605 610 aag tagccatgac tgattcccag act 1965 Lys 00 <210> 58 0 <211> 614 0 <212> PRT CK1 <213> Cor ynebact er i um gl ut ai cum NO 0 <400> 58 0 Val Leu lie Val Val G y Val Tyr Ala Leu Val Leu Leu Thr y Asp (C1 1 5 10 Arg Ser Ala Thr Pro Lys Leu Gy I e Asp Leu G n Gy y Thr Arg 25 Val Thr Leu Val Pro G n y G n Asp Pro Thr G n Asp G n Leu Asn 40 Sn Ala Arg Thr lie Leu u Asn Arg Val Asn y IVet y Val Ser 55 G y Ala Ser Val Val Ala Asp G y Asn Thr Leu Val lie Thr Val Pro 70 75 Gy u Asn Thr Ala n Ala n Ser Leu Gy n Thr Ser n Leu 90 Leu Phe Arg Pro Val G y G n Ala y IVet Pro Asp IVet Thr Thr Leu 100 105 110 IVet Pro G u Leu G u G u Iet Al a Asn Arg Trp Val G u Tyr G y Val 115 120 125 Ile Thr u u n Ala Asn Ala Ser Leu u u IVet Asn Thr Ala 130 135 140 Val Ala Ser Thr Thr Al a Val G u y G u G u Ala Thr G u Pro G u 145 150 155 160 Pro Val Thr Val Ser Al a Thr Pro IVet Asp G u Pro Ala Asn Ser lie 165 170 175 Su Ala Thr On Arg Arg On Gu lie Thr Asp IVet Leu Arg Thr Asp 180 185 190 Arg G n Ser Thr Asp Pro Thr Val n Ile Ala Ala Ser Ser Leu IVet 195 200 205 G n Cys Thr Thr Asp G u IVet Asp Pro Leu Al a G y Thr Asp Asp Pro 210 215 220 Arg Leu Pro Leu Val Al a Cys Asp Pro Ala Val y y Val Tyr Val 225 230 235 240 Leu Asp Pro Al a Pro Leu Leu Asn G y G u Thr Asp G u G u Asn G y 245 250 255 Page Ala Ar g L eu Th r G31y As n 31u 1 1 260 seqi i st car r ect ed. t xt e Asp Thr Asn Arg Pro 265 I I e Th r (3 y 270 G31y Ser O31n 305 Ser I Ie Le u Ar g Le u 385 li1e Thr G31y I Ie Ar g 465 Pr o ValI ValI ValI As n 545 Ly s Tyr Thr As n As n 275 As p Le u Pr o G31y Tyr 355 G31y A a ValI Ph e Tr p 435 G31y Ly s A a Le u G31y 515 Thr Ph e ValI Ly s As n 595 (31u O31n O31u O31n I Ie 325 Ph e A a Thr Le u A a 405 A a G31y G31y O31u O31u 485 O31y A a Le u A a O31u 565 li1e Ser (31u G31y G31y 295 li1e Ser O31n Pr o Thr 375 A a Tyr ValI Ser Thr 455 Ar g A a I Ie Thr Thr 535 Ser Ar g A a As p O31n 280 Ser A a A a Thr Le u 360 ValI O31y Ar g Le u Le u 440 A a O31u Ly s ValI Le u 520 A a Ser A a Ly s As n 600 vb t A a I Ie Thr O31u 345 Ser Pr o I Ie ValI ValI 425 As p As p O31y Ar g I Ie 505 O31y Pr a ValI As n As n 585 Ser O31u Thr Thr Pr a 330 A a Ph e Pr a ValI Ph e 410 Tyr Le u Ser Ar g Thr 490 Tyr Le u Le u As n O31y 570 A a O31u I Ie Tr p Le u 315 ValI O31n A a Ser O31y 395 O31y O31y A a Ph e Ser 475 li1e Le u Thr ValI O31y 555 O31u A a A a Ser Ser 300 As p O31y As p O31y Le u 380 li1e Ph e Le u O31y ValI 460 Ph e ValI Le u Thr I Ie 540 vb t Le u A a Pr a Ph e 285 Ser Ser Ser Le u O31u 365 O31y A a ValI Le u I Ie 445 ValI Ar g Thr A a ValI 525 Le u O31y As p As p O31y 605 A a Le u O31n A a A a 350 As n A a Le u Ser ValI 430 A a Ph e Ser O31y ValI 510 Ph e A a Ar g O31u Ly s 590 Thr Ph e Thr ValI Thr 335 As n O31y A a ValI Le u 415 Le u O31y Tyr A a As n 495 O31y As p Ser ValI Pr a 575 A a As p Ly s Ser I Ie 320 Ser As n O31u Ser A a 400 Ph e Le u Le u O31u ValI 480 vb t O31u Le u Ar g vb t 560 O31u Se r Th r Page 81 seqi i st car r ect ed. t xt <210O> 59 <21 1> 819 <21 2> DNA <21 3> COr ynebact er i urn gi ut arr cumn <220> <221 CDS <222> (796) <223> RXN00046 <400> 59 t ggt gcccaa gcagccgt ca t cgcagcagc aaaat at gcc acct aaaacg ct gt t ct caa cacaggagt t t cct t aaat a cgcgat aacg cctt t taagc atg gac tta aat act 11! l'bt Asp Leu Asn Thr c aa O31n gt g ValI cat Hi s ggc G31y g ac As p at c I Ie act Thr ac c Thr at g G31y 1 50 gag (31u tt a Le u t ca Ser gct A a gcc A a t ac Tyr ccc Pr o cca Pr o gt c ValI gca A a 120 g ac As p g at As p ac a Thr ccc Pr o ct c Le u g ac As p gcc A a ggt G31y Ph e ag g Ar g gcg A a ct g Le u gca A a gt g ValI ggt G31y 1 70 gcg A a t ac Tyr G31y t gt Cy s t t g Le u gga G31y 75 g at As p g at As p gt c ValI act Thr t cc Ser 155 cca Pr o t t c Ph e gca A a cat Hi s gt c ValI g aa (31u ct c Le u t cc Ser gca A a act Thr ccc Pr a 140 ac c Thr g at As p ct c Le u c ag O31n gcc A a g at As p 45 g at As p ac c Thr gt t ValI act Thr gt t ValI 125 g aa O31u ac g Thr tt c Ph e at t I Ie ct t Le u at g vb t ggc G31y at c I Ie aaa Ly s cga Ar g tt c Ph e 110 gcc A a g aa O31u ct t Le u g at As p ggc G31y 1 90 c aa O31n 15 cga Ar g ggt G31y cgt Ar g g aa (31u gca A a 95 cga Ar g c ac Hi s gt t ValI gct A a t gc cys 1 75 g aa (31u ggc G31y g at As p gct A a t ca Ser ggc G31y 80 gt g ValI cct Pr a g at As p ct c Le u gga G31y 1 60 ct c Le u ggt G31y ct c Le u cat Hi s gcc A a 50 t cc Ser g aa (31u g aa (31u cct Pr a gga G31y 130 gcg A a ac c Thr g aa O31u tt c Ph e at t I Ie ac g Thr 35 at t I Ie aac As n gga G31y t cc Ser g ac As p 115 at t I Ie cat Hi s g aa (31u gca A a t cc Ser 1 gt t ValI 20 ct c Le u cgc Ar g cgt Ar g gt t ValI ggc G31y 1 00 ggc G31y ct c Le u aag Ly s c ac Hi s cgt Ar g 1 aac As n t cc Ser ac c Thr t gt cys gt c ValI t ac Tyr gcc A a t cc Ser at c I Ie gct A a cgc Ar g 1 gag O31u cct Pr a gcg gat gt g gcg cac ggt cgt ct c at t ggt gcc aac gcg at c at c gt g Page 82 seqI i st car r ect ed. t xt Al aAs pVal Ala His Oy Arg Leu Ile OyAlaAsn Ala Ilie IleVal 200 205 210 ggc acc gca at c act gac cct ggt t tc at c act gga cag t tc gcg t ca 787 G31y Thr Al a I Ie Thr Asp Pr o G31y Ph e I Ie Thr G31y O31n Phe Al a Ser 215 220 225 ct g t tg cac t agcact tag t ccagcgct g cac 819 Leu Leu Hi s 230 <210> <211> 232 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn <400> I'bt Asp Leu Asn Thr O31n Ar g Se r Lys Leu Tyr Al a 03 n Leu 03 n 03 y O31n Thr Pr a I Ie Ser Al a Ar g Ser Ser 145 Tyr Ar g Ar g As n G31y 225 I Ie Thr lie As n G31y Ser As p 115 li1e Hi s (31u Al a Ser 1 95 li1e Ph e Ser Thr cys ValI Tyr Al a Ser I Ie Al a Ar g 1 65 (31u Pr a ValI Ser ValI Hi s G31y As p 70 li1e Thr Thr vb t G31y 150 (31u Le u Al a G31y Le u 230 O31n ValI O31y ValI Thr ValI Ph e Al a 135 Al a Ly s ValI As p Thr 215 Le u Al a Al a 40 Tyr Pr a Pr a ValI Al a 120 As p As p Thr Pr a ValI 200 Al a Hi s Pr a Al a O31y ValI Thr cys 1 O31u cys Ph e ValI As p 1 Al a I Ie 10 As p Al a O31y Ph e Ar g 90 Al a Le u Al a ValI O31y 1 70 Al a Hi s Thr O31y cys Le u O31y 75 As p As p ValI Thr Ser 1 55 Pr a Ph e O31y As p Hi s ValI O31u Le u Ser Al a Thr Pr a 140 Thr As p Le u Ar g Pr a 220 Al a As p As p Thr ValI Thr ValI 125 O31u Thr Ph e I Ie Le u 205 (31y Ar g O31y Ar g O31u Al a Ar g Hi s ValI Al a cys 1 O31u Oy le As p Al a Ser O31y ValI Pr a As p Le u O31y 1 Le u O31y Al a Th r <210O> 61 <21 1> 1219 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn Page 83 seql i st cor r ect ed. t xt <220> <221> CDS <222> (48)..(1196) <223> RXN01863 <400> 61 ggtatcatac cgatatgaac caaatagaaa gaaggaagtt taagacg atg aat agc IVet Asn Ser 1 gt c Val t at Tyr ttt Phe ggt G y at t SIle aca Thr aaa Lys 100 aat Asn t at Tyr ttc Phe gct Al a t ca Ser 180 acg Thr aaa Lys ttg Leu gat Asp act Thr act Thr cgt Ar g ggc G y ct a Leu aac Asn gat Asp aat Asn 150 tta Leu caa G n cca Pr o agc Ser caa G n aaa Lys aat Asn caa G n ctt Leu cca Pr o aag Lys aca Thr 120 at g Met ggc Gy tta Leu at g Met tta Leu 200 cac Hi s cct Pr o gaa G u 25 ctt Leu act Thr gat Asp at g bet t at Tyr 105 tt g Leu cat Hi s ttt Phe t at Tyr ttt Phe 185 cga Ar g gag G u gtt Val cat Hi s tgt Cys gt c Val gaa G u ttt Phe gca Al a cct Pr o at c SIle ggc Gy gaa G u 170 gt a Val aaa Lys at t SIle agc Ser ggc Gy aat Asn gac Asp at t SIle 75 tct Ser cat Hi s caa G n t ca Ser gca Al a 155 caa G n tct Ser at a SIle cac Hi s at t SIle caa G n at g let ccc Pr o cca Pr o aaa Lys cat Hi s gat Asp cat Hi s 140 at g Met at g Met gcg Al a cat Hi s cct Pr o 220 t ac Tyr tta Leu cgt Ar g 45 gag G u aat Asn aag Lys cga Ar g cgt Ar g 125 aac Asn aag Lys at t SIle gaa G u caa G n 205 at g let aat Asn act Thr 30 tgt Cys cct Pr o ct g Leu tct Ser ggt G y 110 t at Tyr tgg Tr p aag Lys tcg Ser aca Thr 190 gaa G u t at Tyr gat Asp tta Leu agc Ser tta Leu cga Ar g at t SIle at a SIle tta Leu gga G y caa G n aat Asn 175 at g Met gt c Val cca Pr o cca Pr o agt Ser cac Hi s gat Asp acg Thr aga Ar g t at Tyr gat Asp aca Thr cca Pr o 160 gca Al a ct c Leu gtt Val gct Al a tgg Tr p aac Asn tgt Cys at g Met at g Met aat Asn aca Thr at t SIle act Thr 145 ccg Pr o cgt Ar g aat Asn cat Hi s gac Asp 225 gaa G u at c SIle gca Al a gac Asp t ca Ser gtt Val caa G n gct Al a 130 gat Asp tta Leu aca Thr caa G n gat Asp 210 ttt Phe t ca Ser gaa G u gtt Val tta Leu att SIe gtt Val at g Met 115 gaa G u gaa G u aaa Lys tta Leu agt Ser 195 at g let gca Al a agt caa tta aat gtg tta act cta gcg gaa atg aaa aag aca att cat Page 84 seqi i st car r ect ed. t xt Ser O31n Leu Asn Val Leu Thr Leu Ala O31u l'bt Lys Lys 230 235 240 Th r I Ie Hi s g at As p tt g Le u 260 t ca Ser ggc G31y act Thr aaa t ca Ser 340 ct t Le u cgt Ar g tt g Le u gt g ValI tt a Le u agt Ser tt c Ph e 310 ac a Thr aat As n aaa Lys5 at g vb t tt c Ph e cca Pr o aat As n 280 tt a Le u g at As p gt a ValI cat Hi s at g vb t 360 ag a Ar g t gc Cy s 265 gct A a aat As n gaa O31u tt t Ph e t gt Cy s 345 t ac Tyr g at As p 250 tt a Le u aac As n gt c ValI ac a Thr g at As p 330 t cc Ser t at Tyr g aa O31u aag Lys5 aat As n aat As n ggt G31y 315 aaa Lys5 gag O31u ccg Pr o g at As p g at As p gt a ValI gt a ValI 300 ac a Thr t gg Tr p tt t Ph e aat As n at t I Ie 380 t gg Tr p g aa O31u 270 act Thr ac a Thr t cg Ser t cc Ser t gt cys 350 g at As p tt a Le u c aa O31n aat As n aat As n at a I Ie 320 g at As p gga G31y aaa Lys5 tt t Ph e aag Lys5 g ac As p gt a ValI 305 c aa O31n ct t Le u cca Pr o g at As p ggt G31y tt a Le u ccg Pr o 290 at c I Ie aaa gct A a aat As n aat As n 370 824 872 920 968 1016 1 064 1112 1160 1206 1219 aaa caa cca caa Lys On Pro O1n at a caa tt t I I e 03 n Phe t aaaaact ct t aat tat gcg gag <210O> 62 <21 1> 383 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 62 l'bt Asn Ser Val Lys Leu Lys O31n Pro 1 5 Trp O31u Se r Ty r Asn Asp Val Lys O31u As n II e 03 u Phe Thr Thr Thr Asn Leu 40 Cy s Ala Val O31y Ty r Thr Leu O31n Th r l'b t As p Leu I Ie Ty r Ar g Ar g Leu Asp 70 l'b t Ser I Ie Thr O31y 31y O31u Pr o l'bt As n Val Val Lys Pr o Leu Leu Lys Tyr 100 105 Val Ser 10 Hi s 03 y Cys As n Val As p O31u I Ie Phe Ser 90 A a Hi s Page Tyr Le u Ar g O31u As n Lys5 Ar g As p Le u Se r Le u Ar g I Ie lie seql i st cor r ect ed. t xt Thr G n MIet Asn Ser Asn Leu Thr 115 120 Leu Pro G n Asp Arg Tyr Leu Asp 125 SIle Thr 145 Pr o Ar g Asn Hi s Asp 225 Thr Phe Lys Asp Val 305 G n Leu Pr o Asp G u G u Lys Leu Ser 195 lbt Al a Hi s Thr Leu 275 Asp Val Asp Lys Val 355 G u SIle Al a Lys 165 G u Leu Cys G n SIle 245 Pr o Ar g Ar g Asp Leu 325 Leu Val Hi s Val 135 Val Lys Sy Hi s Ar g 215 Asn Asp Phe Ar g Ar g 295 Sy Asp Cys Asn Hi s 375 Hi s Phe Tyr Phe 185 Ar g G u Leu Ar g Cys 265 Al a Asn G u Phe Cys 345 Tyr G n Ser Al a 155 G n Ser SIle Hi s Leu 235 G u Lys Asn Asn Sy 315 Lys G u Pr o G n Hi s 140 Met Met Al a Hi s Pr o 220 Al a Asp Asp Val Val 300 Thr Tr p Phe Asn SIle 380 Asn Lys SIle G u G n 205 lb t G u SIle Asp Thr 285 Phe SIle Leu Ser l t 365 I le Sy G n Asn 175 lbt Val Pr o Lys Met 255 Asp Ar g Sy Asn Ser 335 Leu Phe Phe Thr Pr o 160 Al a Leu Val Al a Lys 240 Leu G n Asn Asn SIe 320 Asp Sy Lys <210> 63 <211> 618 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(595) <223> RXN00833 <400> 63 agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct tttctagcac caaaacaaaa ctctccctag tatggggtcc atg gct aaa aca cat 115 lMt Ala Lys Thr His Page 86 IO seql i st cor r ect ed. t xt 1 ttt caa ggc aac gaa act gct acc tcc ggc gaa ctg cca cag gtc ggc 163 Phe n y Asn u Thr Ala Thr Ser Gy u Leu Pro n Val y 10 15 d) Sgac aac ctc gca gag ttc aac ctc gtc aac acc gaa ctg ggc gag gtc 211 Asp Asn Leu Ala G u Phe Asn Leu Val Asn Thr G u Leu y G u Val 25 30 tcc tca aag gac ttc cag ggc cgc aag ctt gtc ctg aac at c ttc cca 259 Ser Ser Lys Asp Phe G n y Arg Lys Leu Val Leu Asn Ile Phe Pro 45 00 tcc gtt gac acc ggc gtt tgt gca aca tca gtc cgc aag ttc aac gag 307 0 Ser Val Asp Thr y Val Cys Ala Thr Ser Val Arg Lys Phe Asn G u 60 (N \C gca gca gca agc ctg gaa aac acc acc gtg ctg tgc atc tcc aag gat 355 0 Ala Ala Ala Ser Leu G u Asn Thr Thr Val Leu Cys Ile Ser Lys Asp S 70 75 80 ctt cca ttc gca ctg ggc cgt ttc tgc tcc gca gaa ggc atc gag aac 403 Leu Pro Phe Ala Leu G y Arg Phe Cys Ser Ala G u y Ile G u Asn 95 100 gtc acc cca gta tcc gca ttc cgt tcc acc ttc ggt gaa gac aac ggc 451 Val Thr Pro Val Ser Al a Phe Arg Ser Thr Phe Gy G u Asp Asn y 105 110 115 atc gtg ctc gaa ggc tca cca ctt aag ggt ctt ctt gca cgc agc gtc 499 lie Val Leu G u y Ser Pro Leu Lys y Leu Leu Ala Arg Ser Val 120 125 130 atc gtc gtc gat gaa aac ggc aag gtt gct tac acc cag ttg gtt gat 547 ile Val Val Asp G u Asn G y Lys Val Al a Tyr Thr G n Leu Val Asp 135 140 145 gag atc ttc act gaa cct gat tac gac gct gca ctt gct ggg ctg aac 595 G u Ile Phe Thr G u Pro Asp Tyr Asp Ala Ala Leu Ala y Leu Asn 150 155 160 165 taatttactt cgctcagggg aat 618 <210> 64 <211> 165 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 64 Met Ala Lys Thr His Phe n Gy Asn u Thr Ala Thr Ser Gy u 1 5 10 Leu Pro G n Val y Asp Asn Leu Ala G u Phe Asn Leu Val Asn Thr 25 G u Leu y G u Val Ser Ser Lys Asp Phe n y Arg Lys Leu Val 40 Leu Asn lie Phe Pro Ser Val Asp Thr y Val Cys Ala Thr Ser Val 55 Arg Lys Phe Asn G u Al a Ala Ala Ser Leu G u Asn Thr Thr Val Leu 70 75 Cys lie Ser Lys Asp Leu Pro Phe Ala Leu G y Arg Phe Cys Ser Ala 90 Page 87 31u Gy1 lie O31u 1 00 (31y 1u As pAs n 115 Leu Al a Ar g Ser 130 Thr G3 n Leu Val 145 Leu Al a G3 y Leu seqI i st car r ect ed. t xt Asn Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe 105 110 G31y I Ie Val Leu O31u G31y Se r Pr o Leu Lys G31y Leu 120 125 Val I Ie Val Val As p O31u As n G31y Lys ValI Al a Ty r 135 140 As p O31u IIe Phe Thr O31u Pro As p Ty r Asp Al a Al a 150 155 160 As n 1 <210O> <21 1> 879 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (856) <223> RXN01676 <400> agt t acagct t t t ct cggt g gcacact cgc gct act t agc accagcat tt t t tgcat cct cagt gggt gc t ggcccgcgc gt t ValI ggt G31y at c I Ie tt c Ph e ct g Le u ct a Le u ggc G31y gca Al a tt c Ph e gga G31y gt t ValI ggc G31y t ct Ser at g vb t gt t ValI at t I Ie 120 gca Al a ct c Le u t cg Ser tt c Ph e gcc Al a agt Ser act Thr agt Ser gga G31y G31y at c I Ie gac As p act Thr 75 ag t Ser t t g Le u gct Al a ct t Le u gag (31u gt g ValI tt c Ph e gt g ValI at t I Ie gct Al a t at Tyr ct a Le u ct g Le u ct g Le u 45 gga G31y ac c Thr gcc Al a gcc Al a ggt G31y 125 gt a ValI tt t Ph e at t I Ie aaa Ly s t ca Ser ggt G31y ac c Thr 110 gcg Al a ct t Le u 15 at c I Ie at c I Ie gca Al a ggt G31y tt t Ph e 95 agt Ser gga G31y ct c Le u ac c Thr ct a Le u ct c Le u ct a Le u 80 t gt cys gga G31y at g vb t cct tgt gccg cact cct t tt at g at c ct t cac ggt 11! I'b t I Ie Leu Hi s G31y 1 gt g cca ct t ggc ct t 16& Val Pr o Leu G31y Leu caa cgc cag acc atc 21 O31n Ar g O31n Thr Ilie ggt t tt gt c cag at c G31y Phe Val O31n Ilie cca gga t ta gat cgt Pro G31y Leu Asp Ar g gga aag agc t tt t ta 03 y Lys Ser Phe Leu t cc gga cca at c ct c Ser G31y Pr o Ilie Leu 1 00 aac t cc at c acc t ca Asn Ser Ilie Thr Ser 115 gt g ct g ccc ct g at g 49! Val Leu Pr o Leu I'b t 130 gct att gca gcg ctc tgg gcc aaa ctc gga cag cgt gga cag cag atg Al a IlieAl aAla Leu Trp Ala Lys Leu 0 y O n Arg 0 y On 0On I'bt Page 88 INO seql i st cor r ect ed. t xt 135 140 145 ctc cgc ggc cgg gaa ttc acc ttc ttg ggc agg cag tgg cac att gtt 595 Leu Arg G y Arg G u Phe Thr Phe Leu G y Arg G n Trp His lie Val 150 155 160 165 4 tct gtc att agc ggt gcc ctg atc atc gct gtc gga atc ctc ttt tgg 643 Ser Val lie Ser y Al a Leu Ile I e Ala Val y Ile Leu Phe Trp 170 175 180 tcc acg aac ggc ctt gtc agc atg ccg gag ctc gtt cca atg gac acc 691 Ser Thr Asn y Leu Val Ser IVet Pro G u Leu Val Pro IVet Asp Thr 185 190 195 00 cag atc tgg cta cag gaa gcc aca ttc tca ctc ggg tca cca ctc ttt 739 0 G n I le Trp Leu n G u Ala Thr Phe Ser Leu y Ser Pro Leu Phe 200 205 210 gac atc gca ttg atc att gtc gcc gct ggc ttg ttc ttg tac ttc tgg 787 SAsp lie Ala Leu Ile I le Val Ala Ala y Leu Phe Leu Tyr Phe Trp 215 220 225 aac aaa cga caa aag cga aaa gaa gaa gct cag cga ccc aaa gaa agt 835 Asn Lys Arg G n Lys Arg Lys G u G u Ala n Arg Pro Lys u Ser 230 235 240 245 gga tgg gtt att aac cct cgc taattattag ttttggagcg agg 879 Sy Trp Val lie Asn Pro Arg 250 <210> 66 <211> 252 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 66 IVet I e Leu Hi s G y Val Val Phe Tyr Al a G y Leu Leu Val Leu Leu 1 5 10 Val Pro Leu y Leu y Ala Gy lie Leu y G u Leu Phe lie Thr 25 Sn Arg n Thr Ile Ile Val Val Ser Ser Ile Val Leu Ile Ile Leu 40 Sy Phe Val n Ile Phe y y y Phe Asp Phe y Lys Ala Leu 55 Pro y Leu Asp Arg Leu G n Ser Lys Ala Thr Val Thr Ser y Leu 70 75 G y Lys Ser Phe Leu Leu G y bet Thr Ser Ser Ile Al a y Phe Cys 90 Ser y Pro Ile Leu G y Ala Val Leu Thr Leu Ala Ala Thr Ser y 100 105 110 Asn Ser Ile Thr Ser Ala Leu Ile Leu Ser Ala Tyr y Ala y IVet 115 120 125 Val Leu Pro Leu IVet Al a I e Al a Al a Leu Trp Al a Lys Leu G y G n 130 135 140 Arg y n n IVet Leu Arg y Arg G u Phe Thr Phe Leu y Arg 145 150 155 160 Sn Trp His Ile Val Ser Val Ile Ser y Ala Leu Ile Ile Ala Val Page 89 03 y I I e Leu Val Pr o N/t 1 95 O31y Ser Pr o 210 Phe Leu Tyr 225 Ar g Pr o Lys 1 65 Tr p Th r Ph e Tr p Ser 245 seqI i st car r ect ed. t xt 170 175 Ser Thr As n O31y Leu Val Ser I'b t Pr o O31u Leu 185 190 (31n I Ie Tr p Leu O31n G31u Ala Thr Phe Ser Leu 200 205 As p I IeAia Leu I Ie I Ie Val Ala Ala (1y Leu 215 220 As n Lys Ar g O31n Lys Ar g Lys O31u O31u Ala O31n 230 235 240 O31y Tr p Val Ilie Asn Pr o Ar g 250 <210O> 67 <21 1> 744 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (721) <223> RXN00380 <400> 67 caggcaat gc gacct cgcct cagt gacat c ct t ggt gt t c cgt gcat tac aacgaaccag ct caggagat t tgat cact c caagacgat c aaat tgt cgg ct a gca gca Leu A a A a gcc t gc agc A a Cys Ser gga acc ttc O31y Thr Phe gac gag gct Asp 03 u A a atg gaa gag [vb t O31u (1u gt c at c ct c Val Ilie Leu t cc gat gat Ser Asp Asp ggc gac acc O3 y Asp Thr 120 at c lIe g ac As p tt c Ph e cgt Ar g ac a Thr gcg A a c ag O31n ggt (31y ggc O31y ag t Ser cac Hi s caa O31n cag O31n 75 t gg Tr p at t O31y t gc Cys ac c Thr t cc Ser c aa O31n 60 at c I Ie ggg O31y at c I Ie ac c Thr gt g ValI gct A a ccg Pr o 45 ct c Le u aac As n c ag O31n cat Hi s gt g ValI 125 ac a Thr ggt O31y g at As p ccc Pr o ct g Le u t gg Tr p gag O31u 110 tt g Le u ag c Ser g ac As p aag Ly s at t I Ie g at As p 80 gca A a ct c Le u at c I Ie gt g ValI 1 gga O31y gct A a at g vb t ggt O31y 65 tt c Ph e ccg Pr o c aa O31n aat As n ct t Le u gt t ValI g aa O31u 50 gga O31y g aa O31u t gc Cys gct A a gt g ValI 130 gcg A a gct A a 35 at t I Ie g at As p aac As n cgc Ar g gcc A a 115 cgt Ar g ct a Le u 20 gt c ValI tt c Ph e t cc Ser c aa O31n t cc Ser 1 00 gga O31y g at As p cgt t tg acc aaa Ar g Leu Thr Lys gt a ValI O31y t ac Tyr ct c Le u gt t ValI g aa O31u aac As n t ac Ty r t cc cgc gac at c gcc caa gac t tt gt c acc gac aac ggc ct t gat t ac Page Ser Arg Asp IlieAla On Asp Phe\ 135 140 cca agc at t t ac gat cca cca t tt a Pr o Ser I Ie Ty r As p Pr o Pr o Phe 150 155 gt t ccc gca t cg gt g at c cca acc a Val Pr o Al a Ser Val I Ie Pr o Thr T 1 70 cgc ccc gca gca gt g t tc t tg cgc g Ar g Pr o Al a Al a Val Phe Leu Ar g C 185 1 gat gt t gcg t tg cca t tg gt a gat g As p Val Al a Leu Pr o Leu Val As p C 200 205 ag c <210O> 68 <21 1> 207 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn Iist cor r ect ed. t xt (al Thr As p Asn O31y Leu Asp Tyr 145 .tg aca gca gca t cc ct c ggt ggt /bt Thr Al a Al a Ser Leu G31y G31y 160 165 cc at c gt g ct g gat aaa cag cac hr I Ie Val Leu As p Lys O31n Hi s 175 180 iaa gt c acc t cc aaa gat gt g tt g Su Val Thr Ser Lys As p Val Leu 90 195 iag gcc t aaat gt ct g agat t gt ggt Su Al a <400> 68 Val Ar g Leu Thr (3 y Leu Al a Leu Al a Val Al a Val I'b t (31u I Ie Phe O3 y 03 y As p Se r Phe 03 u As n 03 n Pr o Cys Ar g Ser 1 00 (3 n Al a Al a 03 y 115 As n Val Ar g As p 130 Asn 03 y Leu Asp 145 Al a Ser Leu O31y Leu Asp Lys 03 n 1 80 Ser Lys Asp Val 1 95 Lys Leu Al a Val Al a Cys O3 y 03 y Thr Tyr Asp 03 u Leu I'b t 03 u 70 Val Val Ilie O3 u Ser Asp Asn (1y Asp Tyr Ser Ar g 135 Tyr Pr o Ser 150 O31y Val Pr o 1 65 Hi s Ar g Pr o Leu Asp Val Al a Ser Ph e 40 Al a O31u Le u As p Thr 120 As p I Ie Al a Al a Al a 200 Thr Ser O31n As p G31y As n Le u 1 Pr o I Ie Tyr Ser Al a 1 Le u lIe 10 As p Ph e Ar g Thr Al a 90 O31n G31y Al a As p ValI 1 70 ValI Pr o O31y Ser Hi s O31n O31n Tr p I Ie O31y O31n Pr o 1 li1e Ph e Le u ValI Al a Pr o Le u As n O31n Hi s ValI 125 Ph e Ph e Thr Ar g As p 205 Le u Thr O31y As p Ser Cys O31u O31y Th r Th r I Ie 1 ValI Al a Page 91 seqi i st car r ect ed. t xt <210O> 69 <21 1> 495 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm curn <220> <221 CDS <222> 101). (472) <223> RXN00937 <400> 69 agctgccggt caatgaagaa aatccttggc cgggaataac t ctatat at agacct tacaa at ct tgaacg gagat tct ta t acagt ccgc t gaaagt tgg gt a ValI ct c Le u cca Pr o aag Ly s c ag O31n cgc Ar g c ag O31n c ag O n g aa O31u g ac As p t ac Ty r g at As p at c I Ie gcc Al a aag Ly s ggt O31y 120 ac a Th r t gg Tr p aag Ly s g aa O31u act Th r ac c Th r ct c Le u tt t Ph e gca Al a gt t ValI gca Al a ct g Le u 75 at g vb t gac As p gag O31u t cc Ser t cc Ser aac As n at g vb t cca Pr o at g vb t ag c Ser t gg Tr p g aa O31u 45 c ag O31n gt t ValI gct Al a g at As p ac a Th r t gc cys ac c Th r ggc O31y Ph e cct Pr o g ac As p 110 gt t ValI 15 gga O31y c ac Hi s ct g Le u cgc Ar g gca Al a 95 gt t ValI ac c Th r cct Pr o ac c Th r gct Al a g ac As p ct g Le u cgt Ar g at g vb t 1 ggc O31y t gc cys g ac As p gca Al a ggc O31y g at As p cgc Ar g g ac As p cgc Ar g gca Al a gca Al a at c I Ie g at As p c ag O31n gga G31y c ag O31n ac c Th r ct g Le u at g vb t ct g Le u gt c ValI 115 at t lIe tt c Ph e tt c Ph e c ag O31n gt c ValI gt c ValI 1 00 gca Al a gca acc at c Al a Th r I Ie t ct gca gag gca Ser Al a 0u Al a t aagct tcca at tgtgt t tt ggt <210O> <21 1> 124 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm curn <400> l'b t Al a Thr I Ie Asp Val Thr O31u O31u Thr Phe O31u Ser Thr Val Thr 1 5 10 Oy Asp Oy IleVal Leu Val Asp Al aTr pAl aSe r TrpCys Oy Pro 25 Cys Ar g O31n Ph e Al a Pr o Thr Ty r O31u Lys Val Ser Ou Thr His Thr 40 As p Al a Th r Phe Al a Lys Leu As p Thr O31u Al a Asn O31n O31y L eu Al a 55 Page 92 seqI i st car r ect ed. t xt Ala Ala Leu On Ilie On Ser Ilie Pro Thr Leu I'bt Val Phe Arg Asp 70 75 O31y I Ie I'b t Val Tyr Ar g O31u Al a O31y Th r I'b t Pro Al a Pro Al a Leu 90 Asp Asp Leu Val Asn (1n Val Lys AaLeu Asp 'b t As pAsp Val Ar g 100 105 110 Ar g O31n ValI Al a O31u O31n O31n O31y Ser Al a O31u Al a 115 120 <210O> 71 <21 1> 990 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (967) <223> RXN02325 <400> 71 cagagatttg aagatggaga ccaaggctca aagggaatcc t gcacccgt c t aat gaaaat cat tact at t aggt gt cat g at gccgt ct t ggt t taat ac atg gac cat gca cac 11! I'bt Asp Hi sAl aHi s g at As p ct c Le u gt t ValI ac g Thr ct g Le u gca Al a agt Ser t ac Tyr aag Ly s t gc cys cct Pr a aat As n gcc Al a ccc Pr a aat As n g at As p gt g ValI 120 ccg Pr a cca Pr a aaa Ly s ac g Thr aaa Ly s tt t Ph e ggg O31y gt t ValI g at As p gca Al a act Thr gct Al a aag Ly s gct Al a cag O31n 75 cga Ar g gcc Al a ggc O31y caa O31n ct g Le u gt c ValI gt g ValI aag Ly s g at As p tt t Ph e ag a Ar g gcc Al a t gg Tr p 140 cgt Ar g tt a Le u t cc Ser gt g ValI g aa O31u gt a ValI ag g Ar g 110 at t I Ie g at As p g at As p 15 gca Al a t ca Ser gt g ValI aaa Ly s gcg Al a 95 cca Pr a gcg Al a cat Hi s tt g Le u gcg Al a aat As n gt g ValI g ac As p at t I Ie aaa Ly s gag O31u tt t Ph e gt c ValI c ac Hi s gag O31u at t I Ie ct g Le u g at As p at t I Ie aac As n 130 t cg Ser ggc O31y ggg O31y ac c Thr c ac Hi s tt t Ph e g at As p 1 00 gt g ValI ct c Le u g aa O31u at c ccc aat gaa aaa gag ggg gac gt c gat aag caa Ilie Pro Asn Ou Lys Ou Oy Asp Val Asp Lys On Page 93 at a gac ccg cgc I Ie Asp Pro Ar g seqI i st car r ect ed. t xt 1 1 50 ct g t gg cgg Leu Trp Arg t tg gcg tt g Leu Al a Leu cac gcg gcg Hi s Al a Al a 200 gag gat ccg O31u Asp Pr o 215 gcg ct g gcg Al a Leu Al a 230 gcg ct C gcg Al a Leu Al a cgg at c gt g Ar g Ilie Val t tg gaa at c Leu 03 u Il e 280 gcg Al a 1 70 gag O31u t cg Ser gag O31u gcg Al a ct t Le u 250 tt g Le u gcg Al a gaa O31u cag O31n gt a ValI aag Ly s g at As p 235 gca Al a ct g Le u cag O31n tt g Le u tt g Le u tt g Le u t cg Ser 220 at g vb t gca Al a aac As n gt g ValI aac As n C ag O31n 1 90 cgg Ar g Cgg Ar g gt g ValI tt g Le u tt t Ph e 270 aat As n gcc Al a 1 75 g at As p at g vb t g at As p tt g Le u cca Pr o 255 g at As p gca Al a ggt G31y gcg Al a t ct Ser cca Pr o at g vb t 240 aaa Ly s ccg Pr o at g vb t ttt cgc gcg Phe Ar g Al a 1 gt g aag cgg Val Lys Ar g 1 gcg gat cgg Al a Asp Ar g 210 gat gt g aac Asp Val Asn cag ccg gac O31n Pr o Asp gag gct gcc O3 u Al a Al a 260 gac ct g gt c Asp Leu Val 275 t aagaaaaca gcg Al a gcg Al a gga O31y aag Ly s ac a Thr 245 Cgg Ar g gca Al a ct t taaat at t ct <210O> 72 <21 1> 289 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 72 I'bt Asp Hi s Al a Hi s Asp Ser Cys Ser 1 5 O31u ValI Thr O31y O31n Leu O31n Pro O31u Pro His O31u O31y Lys Val Al a As n I Ie 40 I'b t O31u Hi s Thr I Ie Thr O31n Al a Se r 55 Leu I Ie O31y Hi s Ser Leu Leu Pr o Thr 70 1 1 e Leu Hi s Phe 03 n Al a 03 y As n Lys Val As p Pr o As p Ar g Ser Al a As p Val 100 105 O31n I Ie Pr o Val Al a Ty r Val Val Lys 115 120 Phe As n Ser Leu As n Lys O31u Pr o Val Pr o Thr 10 Lys Al a Thr Lys Lys Al a Phe 03 n O31y Ar g 90 Val Al a As p (1y Al a 03 n Page 94 Le u ValI ValI Ly s As p Ph e Ar g Al a Tr p Ar g As p Thr O31u Le u Ser Ph e Ser 125 Le u As p Al a Ser ValI Ly s Al a Pr a Al a Hi s Val 145 G n Asp Thr Val Asp 225 Asn Pr o Leu Ser Ar g Asp Ar g Lys 195 Asp Val Pr o Al a Leu 275 Thr Ar g 165 Al a Al a Gy Lys Thr 245 Ar g Al a SI e 150 Leu Leu Hi s G u Al a 230 Al a Ar g Leu 135 Pr o Tr p Al a Al a Asp 215 Leu Leu SIle G u seql i st cor r ect ed. t xt 140 Asn G u Lys G u G y Asp 155 Arg Ala Ala G u Leu Val 170 Leu Tyr G u G n Leu Pro 185 Ala Val Ser Val Leu Ala 200 205 Pro lie G u Lys Ser Arg 220 Al a Al a Al a Asp IVet Tyr 235 Ala His Leu Ala Ala Leu 250 Val G u Leu Leu Asn Leu 265 I e Arg Ala n Val y 280 285 Asp Al a 175 Asp bet Asp Leu Pr o 255 Asp Al a Lys 160 Gy Al a Ser Pr o Met 240 Lys Pr o Met <210> 73 <211> 900 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(877) <223> RXN01837 <400> 73 cccccatcat tccctcaagg tgtgaagata cggttaggat ttggacattc tcaaaatcaa gtagcaaggg atcaaactct gaa G u cgg Ar g ct g Leu cgc Ar g gca Al a cgc Ar g cgc Ar g gt c Val acc Thr acc Thr cag G n gaa G u ct g Leu gac Asp gac Asp gcg Al a aag Lys gtt Val gaa G u tac Ty r ctt Leu acc Thr gt c Val gt c Val cag G n t cc Ser aag Lys gtt Val 45 at c SIle cca Pr o cag G n cca Pr o ggc Gy acc Thr ct g Leu ctg gag Leu G u 15 ct a acc Leu Thr ggt atc G y lle gct gat Al a Asp gcg ctg Al a Leu Page agaaaagaat ttttttgacg gtg agt act aat aag Val Ser Thr Asn Lys 1 aaa gaa atc aaa agc Lys G u Ile Lys Ser gtg gtc ttt gct tcc Val Val Phe Al a Ser tgg tac gca gct acc Trp Tyr Ala Ala Thr gaa aca tcc acc acc G u Thr Ser Thr Thr acc cgc acc acc gcg Thr Arg Thr Thr Ala seqI i st car r ect ed. t xt ct c Le u aag Ly s gt g ValI g at As p t cc Ser 1 50 ggc G31y G31y g ac As p aac As n gga G31y 230 ag a Ar g g ac As p gt c ValI gt c ValI 120 t cc Ser ggc G31y t ac Tyr ggg G31y ac c Thr 200 ggc G31y ccc Pr o cct Pr o gt g ValI aag Ly s ct g Le u t cc Ser t ac Tyr ct c Le u 1 70 ag c Ser cca Pr o g ac As p ggc G31y aac As n 250 t gt cys gct Al a ac c Thr t gt cys 140 g at As p t gc cys gcc Al a at c I Ie aac As n 220 g aa (31u cga Ar g gag (31u act Thr gcc Al a 125 ac c Thr act Thr ggc G31y aac As n t ac Tyr 205 ggg G31y ct a Le u cgc Ar g t ac Tyr g aa (31u 110 c ag O31n gt c ValI gt c ValI g at As p g aa O31u 1 90 gag O31u ct c Le u c ac Hi s cat Hi s cca Pr o 95 aac As n ggc G31y aac As n t gc cys cca Pr a 1 75 t ac Tyr cgc Ar g cca Pr a ct a Le u cgc Ar g 255 g at As p gt g ValI aac As n gct Al a c ac Hi s 1 ag c Ser cca Pr a G31y gt t ValI ct t Le u 240 ag a Ar g gct Al a cca Pr a at c I Ie gt t ValI 145 cgc Ar g ag c Ser ac c Thr ac c Thr ct t Le u 225 cgg Ar g agt Ser ggc G31y gca Al a ggc G31y 130 gag O31u at c I Ie ac c Thr g ac As p at c I Ie 210 cct Pr a cca Pr a t gg Tr p gag O31u ac c Thr 115 at g vb t c ac Hi s ac c Thr ggc G31y g aa O31u 1 gcc Al a c aa O31n g at As p c ac Hi s gct Al a 1 00 ggc G31y g aa O31u at g vb t ac c Thr gca Al a 1 gca Al a at g vb t ct a Le u c ac Hi s t gaaggt gga accggcgacg gag <210> 74 <211> 259 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 74 Val Ser Thr Asn Lys O31u Arg Arg O31n O31n Al a Leu Ser O31n Leu O31u 1 5 10 Lys O31u I Ie Lys Ser Arg Asp Arg Lys Ou Lys Thr Lys Pro Leu Thr 25 Val Val Phe Ala Ser Leu Al a Val Ilie Leu Val Val Val G31y G31y I Ie 40 Tr p Tyr Al a Al a Thr Ar g Ser Thr O31u Asp O31u Va I lie Th r Al a As p 55 O31u Thr Ser Thr Thr Al a O31u Thr Pr o Asp Ty r O31n Pr o Leu Ala Leu Page 96 seqI i st car r ect ed. t xt Leu G3 y Asp Ser Thr Cys G3 u Tyr Th r A a Pr o I Ie ValI 145 Ar g Ser Th r Th r Le u 225 Ar g Ser Th r G31u Th r 115 vb t Hi s Th r G31y (31u 1 95 A a O31n As p Hi s A a Ser Th r Le u A a Ser 1 65 G31y Th r A a Ar g Ar g 245 As p Th r Ar g 135 O31u I Ie Pr o Le u A a 215 Ph e Ar g Ser 1 As n ValI Ty r ValI Ph e 1 Th r A a Th r Cys Pr o Th r Pr o As n 1 O31n Ph e ValI Th r Th r 235 Pr o A a Th r Cys 140 As p Cys A a I Ie As n 220 O31u Ar g Th r A a 125 Th r Th r G31y As n Tyr 205 G31y Le u Ar g Pr a As n G31y As n Cys Pr a 1 Tyr Ar g Pr a Le u Ar g 255 <210O> <21 1> 741 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (741) <223> RXN01926 <400> ctg cga agc ttc tac acc cca gaa caa Leu Ar g Ser Phe Tyr Thr Pr o O31u O31n 1 5 gtc tgg aaa gcc gcc acc gaa gaa gca Val Trp Lys Ala Ala Thr O31u O31u Ala 25 gcc gtc cac gac cag gaa ctc ttc ctc Ala Val Hi s Asp O31n O31u Leu Phe Leu 40 at c t tc gcc t cc gcg at g ct c aac tt c Ilie Phe Ala Ser Ala I'b t Leu Asn Phe 55 acc ctc tgc caa ctc gca cca tcc ccc Thr Leu Cys O31n Leu Ala Pr o Ser Pr o gcc at c gaa A a I I e 03 u 10 gaa ct c ct c 03 u Leu Leu aac t gc acc Asn Cys Thr ggc gt C cac G31y Val Hi s gcc ggc cgc Ala G31y Ar g Page 97 cgc Ar g gca A a ac c Thr c aa O31n g ac As p g aa O31u gct A a 30 t cc Ser at c I Ie gca A a ggC O31y g ac As p cca Pr a ct g Le u g ac As p seql i st cor r ect ed. t xt aaa Lys acc Thr aaa Lys ttc Phe t cc Ser 145 gaa G u ct c Leu cca Pr o t ca Ser t cc Ser 225 aac Asn ct c Leu gac Asp cac Hi s 115 cgc Ar g aaa Lys gcc Al a cca Pr o cca Pr o 195 ggc Gy ggt Gy ggt Gy gcc Al a t cc Ser gat Asp at g Met gcc Al a ccc Pr o 165 gac Asp ttc Phe t ac Tyr gt c Val ggc Gy 245 gcc Al a ggc Gy acc Thr caa G n ct c Leu 150 ggc Gy acc Thr gcg Al a aaa Lys cag G n 230 cgg Ar g acc Thr gt c Val ct c Leu gt c Val 135 acc Thr gac Asp at c SIle cca Pr o cag G n 215 at c SIle tgt Cys t cc Ser gt c Val gcc Al a 120 acc Thr gt c Val at c SIle ttc Phe gaa G u 200 ttc Phe ct c Leu gca Al a ttc Phe 105 ttc Phe cac Hi s ttc Phe gt c Val gaa G u 185 cac Hi s cgc Ar g aag Lys at g let 90 aaa Lys at g bet t cc Ser ggc Gy ggc Gy 170 ggc Gy ttc Phe aaa Lys aac Asn gac Asp gt c Val cgc Ar g caa G n cgc Ar g 155 ct c Leu cga Ar g cgc Ar g gcc Al a gac Asp 235 cac Hi s gcc Al a gt c Val 125 ggc Sy cgc Ar g aac Asn at c SIle ct g Leu 205 gag G u cgt Ar g cgc Ar g ggc Sy 110 t cc Ser cgc Ar g tct Ser gcc Al a caa G n 190 cgc Ar g cag G n ggc Gy gac Asp at g Met ggc Sy agc Ser acc Thr ggc Sy 175 t ac Tyr gcc Al a ct g Leu gac Asp acc Thr gac Asp gaa G u ttc Phe gtc Val 160 gcc Al a cca Pr o aaa Lys gac Asp gcc Al a 240 <210> 76 <211> 247 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 76 Leu Arg Ser Phe Tyr Thr Pro u n Ala lie u Arg u Gy Asp 1 5 10 Val Trp Lys Ala Ala Thr u u Ala u Leu Leu Ala Ala Asp y 25 Ala Val His Asp n G u Leu Phe Leu Asn Cys Thr Thr Ser Pro Leu 40 Ile Phe Ala Ser Ala IVet Leu Asn Phe y Val His n Ile Leu Asp 55 Thr Leu Cys G n Leu Al a Pro Ser Pro Ala y Arg Asp Ala Asp Pro 70 75 Page 98 Lys Thr Lys Phe Ser 145 G u Leu Pr o Ser Ser 225 Asn Leu Asp Hi s 115 Ar g Lys Al a Pr o Pr o 195 Gy Gy Gy Al a Ser Asp Met Al a Pr o 165 Asp Phe Tyr Val Gy 245 Al a Gy Thr G n Leu 150 Sy Thr Al a Lys G n 230 Ar g Thr Val Leu Val 135 Thr Asp SIle Pr o G n 215 I le Cys seql i st cor r ect ed. t xt Ser Ala IVet Asp Asp His 90 Val Phe Lys Val G n Ala 105 Ala Phe Vet Arg Val Val 120 125 Thr His Ser G n Ser G y 140 Val Phe y Arg Thr Arg 155 Ile Val y Leu Val Asn 170 Phe u y Arg Lys Ile 185 Su His Phe Arg lie Leu 200 205 Phe Arg Lys Ala Leu G u 220 Leu Lys Asn Asp Leu Arg 235 Asp Met Sy Ser Thr Sy 175 Tyr Al a Leu Asp <210> 77 <211> 478 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(478) <223> RXN02002 <400> 77 aagtggcaaa aaacgtttca agcaggcaac gccggcgtac attatggccc agcgcccaca acccgctatt cttaataccc t cc Ser at c SIle ct g Leu ggc gcy ggc acc Thr cac Hi s gca Al a aaa Lys t cc gcc Al a gac Asp at c l Ie acc Thr gcc gcc Al a gcc Al a at c SIle gtt Val t cc gag G u ggt G y t cc Ser t cc Ser t cc gca Al a aaa Lys gaa G u 45 gac Asp gca cat Hi s t cc Ser gcc Al a tgg Tr p ct c cgc cgc Arg Arg 15 acc ct c Thr Leu ggc gcc G y Al a atg gaa Vbet u caa ttc Page 99 aacttcgctg agctggggcg atg agc aac gcc aat IVet Ser Asn Ala Asn 1 aga aca ttc gcc gta Arg Thr Phe Ala Val acc gag gca ttg gcg Thr G u Ala Leu Ala acc cac ggc aaa gca Thr His G y Lys Ala atg gaa aaa gac cgc IVet G u Lys Asp Arg gag tac gca cca gaa G y i e Ser ggc cac gca G y Hi s Al a cac gcc gac Hi s Al a Asp gca gca gt c Al a Al a Val 120 Al a gag G u t cc Ser ctt Leu Ser 75 ccc Pr o gaa Gu at g Met Ser ttc Phe gac Asp cac Hi s seql i st cor r ect ed. t xt Ala Leu n Phe Gu Tyr Ala Pro u 80 atg atc aac ctc gtg gac acc cca ggc IVet I e Asn Leu Val Asp Thr Pro G y 95 100 acc tac cgc gtc ctc atg gcc gtc gac Thr Tyr Arg Val Leu NIet Al a Val Asp 110 115 t cc gtc Ser Val 125 <210> 78 <211> 126 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 78 IVet Ser Asn Ala Asn Ser Asp Thr Thr 1 5 Arg Thr Phe Ala Val Ile Ala His Pro 25 Thr G u Ala Leu Ala Leu His Ala Hi s 40 Thr His G y Lys Ala G y Arg Lys Ala IVet u Lys Asp Arg Gy Ile Ser lie 70 G u Tyr Ala Pro G u G y His Ala G y Val Asp Thr Pro G y His Ala Asp Phe 100 105 Leu bet Ala Val Asp Ala Ala Val bet 115 120 Al a 10 Asp SIle Thr Al a G u 90 Ser Leu Al a Al a SIle Val Ser Pr o G u Mbt Al a Lys G u Asp Al a Met Thr Ser 125 Hi s Ser Al a Tr p Leu SIle Tyr 110 Val Ar g Thr Gy Met G n Asn Ar g <210> 79 <211> 1080 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1057) <223> RXN02736 <400> 79 cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115 IVet Ile Phe G u Leu 1 ccg gat acc acc acc cag caa att tcc aag acc cta act cga ctg cgt 163 Pro Asp Thr Thr Thr G n n lie Ser Lys Thr Leu Thr Arg Leu Arg 15 Page 100 gaa tcg ggc acc cag gtc G u Ser G y Thr G n Val seql i st cor r ect ed. t xt acc acc ggc cga gtg ctc ac Thr Thr y Arg Val Leu Th c ct c at c r Leu Ile gt c Val gaa G u gat Asp gac Asp gct Al a ccc Pr o gac Asp gac Asp 150 gat Asp gcc Al a ct g Leu ttg Leu ccc Pr o 230 cgc Ar g gac Asp tcg Ser gac Asp tcg Ser act Thr ggt Gy aag Lys gtt Val 120 at t SIle gat Asp gac Asp t ca Ser ggt Gy 200 cgg Ar g gt g Val gag G u cat Hi s gt g Val 280 gaa G u gag G u gaa G u t cc Ser cag G n tgg Tr p cgc Ar g gca Al a acg Thr 170 gat Asp agc Ser ct g Leu acc Thr gtt Val 250 ctt Leu at t SIle gat Asp cca Pr o aaa Lys at g Met gt c Val cca Pr o gca Al a 140 gaa G u gcg Al a cca Pr o agt Ser gt g Val 220 gag G u acc Thr gt a Val cgt Ar g gt c Val 45 tct Ser gtt Val at c SI e gt c Val ggt Gy 125 caa G n gat Asp cgc Ar g cca Pr o acc Thr 205 cct Pr o ttt Phe acc Thr gag G u cga Ar g 285 gct Al a cgc Ar g gac Asp at c SIle aca Thr 110 gaa Gu cga Ar g cgt Ar g ctt Leu cac Hi s 190 tcg Ser gt g Val ggt Gy ggc Gy at g let 270 agt Ser gca Al a gt g Val gca Al a at g Met 95 cca Pr o t ca Ser cgc Ar g gtt Val acc Thr 175 agc Ser gt g Val at c SIle act Thr tcg Ser 255 ccg Pr o gag G u gtt Val at c SIle gaa G u cat Hi s ct g Leu cca Pr o at c SIle gag G u 160 cag G n gaa G u gat Asp cgc Ar g cca Pr o 240 at c SIle gaa G u t cc Ser gag G u ttg Leu cgt Ar g aac Asn ctt Leu aat Asn 130 gat Asp t at Tyr cgg Ar g act Thr gct Al a 210 gt g Val ct g Leu at c SIle ggc Gy tgc Cys 290 t cc Ser gt g Val at c SIle gga Gy cct Pr o 115 cct Pr o gct Al a cac Hi s gga Gy t cc Ser 195 gca Al a aca Thr gct Al a acc Thr aat Asn 275 ttg Leu acc Thr gtt Val ggt Gy cct Pr o 100 gac Asp t cc Ser ttg Leu cca Pr o ctt Leu 180 gtg Val ggc Gy gat Asp at c SIe at c SIe 260 gcc Al a tct Ser Page 101 seqi i st car r ect ed. t xt gag ct t cgc cac atg gat cca gat t tg ggc tac cag cac gcact a tcc O31u Leu Ar g Hi s l'bt Asp Pro Asp Leu G31y Tyr O31n Hi s Ala Leu Ser 295 300 305 ggc t t g t cc agc gt c aag ct g gaa acc gt c t aaggagaaa t acaacact a O3 y Leu Ser Ser Val Lys Leu 03 u Thr Val 310 315 t gg <210> <211> 319 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 1 027 1 077 1 080 l'bt I Ie Phe O31u Leu Pro Asp Thr Le1 Le u Thr ValI Le u Le u Ly s Thr 145 As n Tr p I Ie Le u O31u 225 Le u I Ie Ar g Le u Ser ValI I Ie G31y Pr o 115 Pr o A a Hi s G31y Ser 1 95 A a Thr A a Thr Ar g ValI As n O31y O31y ValI Thr O31n Tyr O31y 1 65 ValI Ar g Tr p A a O31n 245 Tyr (31u ValI (31u As p 70 As p A a Pr o As p As p 150 As p A a Le u Le u Pr o 230 Ar g As p Ser Thr A a Ly s A a As p I Ie Pr a 135 Ar g Thr Ser Thr A a 215 Thr Le u A a Thr Thr 10 Thr 03 n 25 Ser 03 u Ar g O31u A a 03 u A a Ser 90 Leu 03 n 1 05 Ala Tr p G31y Ar g As p A a l'b t Thr 1 70 Leu Asp 1 85 A a Ser Arg Leu Pr o Thr I e Val 250 Thr Leu 265 O31n ValI Ser Hi s As n O31u Tyr Tr p I Ie Le u 1 Tr p Hi s O31y Ly s As p 235 Ar g O31n Thr As p Pr a Ly s vb t ValI Pr a A a 140 O31u A a Pr a Ser ValI 220 O31u Thr I Ie Thr ValI Ser ValI I Ie ValI O31y 125 O31n As p Ar g Pr a Thr 205 Pr a Ph e Thr Ser O31y A a Ar g As p I Ie Thr 110 O31u Ar g Ar g Le u Hi s 1 Ser ValI O31y (31y Ly s Ar g A a ValI A a vb t Pr a Ser Ar g ValI Thr 1 Ser ValI I Ie Th r Se r 255 O31n Val O31u l'bt Pro O31u 270 Page 102 seql i st cor r ect ed. t xt Ser y Asn Ala Pro Ser Leu Val Ala Ile Gy Arg Arg Ser u Ser 275 280 285 Asp Cys Leu Ser G u G u Leu Arg His IVet Asp Pro Asp Leu G y Tyr 290 295 300 G n His Ala Leu Ser G y Leu Ser Ser Val Lys Leu G u Thr Val 305 310 315 <210> 81 <211> 331 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(301) <223> RXS03217 <400> 81 tctgtgaagg tagatggttt gacgaggagt tccaacgact cggacgctgg tgaatcat tggcgaacgt agcatcacct gat t aggaaa aggtacaaat atg gca cag ggt act gc Met Ala G n G y Thr 1 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac Val Lys Trp Phe Asn G y u Lys Gy Phe y Phe lie Ala Pro Asn 15 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc Asp G y Ser Ala Asp Leu Phe Val His Tyr Ser G u lie G n y Ser 30 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc Gy Phe Arg Asn Leu Gu Gu Asn Gn Pro Val Gu Phe Gu Val Gy 45 gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc uGu y Ala Lys y Pro n Al a Gn n Val Arg Ala Leu 60 taagctctaa ctgctagcta aaaattccgc <210> 82 <211> 67 <212> PRT <213> Corynebacter i um gl utam cum <400> 82 bet Ala n Oy Thr Val Lys Trp Phe Asn Gy u Lys Gy Phe y 1 5 10 Phe lie Ala Pro Asn Asp y Ser Ala Asp Leu Phe Val His Tyr Ser 25 Gu Ile Gn Gy Ser Gy Phe Arg Asn Leu u u Asn n Pro Val 40 Su Phe u Val y u y Ala Lys y Pro n Ala n n Val 55 Arg Ala Leu Page 103 seql i st cor r ect ed. t xt <210> 83 <211> 324 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(301) <223> FRXA01917 <400> 83 tctgtgaagg tagatggttt gacgaggagt tccaacgact tggcgaacgt agcatcacct gattaggaaa aggtacaaat cggacgctgg tgaatcatgc at g gca cag ggt act Met Ala G n G y Thr 1 gtg aaa tgg ttc aac ggc gaa aag gga ttt ggt ttc atc gct ccc aac Val Lys Trp Phe Asn Gy u Lys Gy Phe Gy Phe lie Ala Pro Asn 15 gat ggc tcc gca gat ctc ttc gtc cac tac tct gag att cag ggc tcc Asp Gy Ser Ala Asp Leu Phe Val His Tyr Ser G u lie G n Gy Ser 30 ggt ttc cgt aat ctt gag gaa aac cag cca gtt gaa ttt gag gtc ggc Gy Phe Arg Asn Leu Gu Gu Asn Gn Pro Val Gu Phe Gu Val Gy 45 gag ggc gcc aag ggc cca cag gct cag cag gtt cgt gct ctc G u y Ala Lys y Pro n Al a Gn n Val Arg Ala Leu 60 t aagct ct aa ct gct agct a aaa <210> 84 <211> 67 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 84 bet Ala n Oy Thr Val Lys Trp Phe Asn Gy u Lys Gy Phe y 1 5 10 Phe lie Ala Pro Asn Asp y Ser Ala Asp Leu Phe Val His Tyr Ser 25 Su lie n y Ser y Phe Arg Asn Leu u u Asn n Pro Val 40 Su Phe u Val Gy u Gy Ala Lys Gy Pro n Ala n n Val 55 Arg Ala Leu <210> <211> 504 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(481) <223> RXA02184 Page 104 seqI i st car r ect ed. t xt <400> t t taccgcga tgct tgcggt gt at aat aat t tct tct ggt gaat cagcat at gaat tagg aat gaaagt g gt gaggacaa caaaaat agt t gat caat t t gt g cct gt c gga aca Val Pro Val Oy Thr ~gtg aag tgg tac gac gcg gag cgt ggt Val Lys Trp Tyr Asp Ala O31u Arg O31y ggt ggt gaa gat t gc t tc gt a ggt aag Gy (1y 1u Asp ys Phe Val G31y Lys 30 00 Sacc gaa t tg cac aag gga cag cga at c SThr O31u Leu Hi s Lys G31y O31n Ar g Ilie 45 Scgt aag ggc cct caa gca ct t cga at a SAr g Lys G31y Pr o O31n Al a Leu Ar g Ilie ~K155 60 agg cgt cca cag cac aaa t ac aag cca Ar g Ar g Pr o O31n Hi s Lys Tyr Lys Pr o 75 t ct gac ct c at c acg ct t ct a gaa agt Ser Asp Leu Ilie Thr Leu Leu O31u Ser aaa ggg caa t ac ccg gag cac aaa gct Lys G31y O31n Tyr Pr o O31u Hi s Lys Al a 105 110 ct t cgc gt t gt t gcg aag gag ct t gag Leu Ar g Val Val Al a Lys O31u Leu O31u 120 125 ccg <210> 86 <211> 127 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 86 Val Pro Val O31y Thr Val Lys Tr p Tyr 1 5 Phe Val Ser As n Pr o O31y O31y O31u Asp Leu Pro Lys O31y Val Thr O31u Leu Hi s 40 Asp Phe Al a Ala O31y Ar g Lys O31y Pro Leu O31u Thr Pr o Ar g Ar g Ar g Pro O31n 70 Leu Asn O31y I'b t I Ie Ser Asp Leu Ilie O31n Pr o G31y Leu Ala Lys G31y O31n Ty r 100 105 tt c Ph e 15 c aa O31n g at As p aag Lys5 g aa O31u gga O31y 95 gga O31y ggc O31y gt a ValI tt t Ph e at t I Ie gag O31u gt g ValI gcg Al a 1 tt t Ph e ct t Le u g ac As p ct t Le u ct c Le u c aa O31n c ag O31n gt c ValI ccc Pr o tt c Ph e g aa O31u aac As n cca Pr o gt a ValI t cc Ser aag Lys5 gcc Al a act Thr gga O31y ggc O31y gca Al a aat As n gga O31y gca Al a cca Pr o at g vb t ct t Le u 1 00 g aa O31u t ct t aaaacaat a aggagaggat Ser As p Al a 10 Cys Phe Lys 03 y O3 n Al a I Hi s Lys Thr Leu 1 90 Pro O31u I Page 105 Ar g O31y Ar g Ar g Lys5 O31u Lys5 Ph e O31n As p Ly s O31u O31y (31y seql i st cor r ect ed. t xt Sn Val Ala u Ile Leu Arg Val Val Ala Lys u Leu u Ser 115 120 125 <210> 87 <211> 324 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(301) <223> RXA00810 <400> 87 tcggcgttcg ttcaagaaac ggccagatgt tgctgttcga tcgaacccgg tagtaattcc aatcagtaaa ggtaagacaa gctcatgcaa gagtggaaca atg gca cag ggc act Met Al a G n y Thr 1 gtt aag tgg ttc aac cca gag aag ggc ttc ggc ttc atc gct cct tcc Val Lys Trp Phe Asn Pro u Lys Gy Phe y Phe Ile Ala Pro Ser 15 gac gga tcc gct gac gtt ttc gtc cac tac tcc gag atc gag ggc aac Asp G y Ser Ala Asp Val Phe Val His Tyr Ser G u lie G u G y Asn 30 ggc ttc cgt acc ctc gag gag aac cag ctc gtc gag ttc gaa atc ggc Sy Phe Arg Thr Leu u u Asn n Leu Val u Phe u lie y 45 gag ggc gct aag ggc ctt cag gct cag gct gtt cgt gca atc G u y Ala Lys y Leu Gn Al a n Ala Val Arg Ala lle 60 taattgcatc tgagttcgaa acc <210> 88 <211> 67 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 88 IVet Ala n Gy Thr Val Lys Trp Phe Asn Pro u Lys Gy Phe Gy 1 5 10 Phe I e Ala Pro Ser Asp y Ser Ala Asp Val Phe Val His Tyr Ser 25 Su lie u y Asn y Phe Arg Thr Leu u u Asn n Leu Val 40 G u Phe G u I e G y G u G y Al a Lys G y Leu G n Al a G n Al a Val 55 Arg Ala lle <210> 89 <211> 1017 <212> DNA <213> Cor ynebact er i um gl ut am cum Page 106 seql i st cor r ect ed. t xt <220> <221> CDS <222> (101)..(994) <223> RXA01674 <400> 89 cggcgtcgat tccagaaggt ttgtagacat gct t caaggt cggtagacgg tactttcata tccacccata taatgttgat tgcgctaatt gaaaagaacg ccg Pr o agc Ser agt Ser gt a Val gtt Val tgg Tr p gcc Al a at g Met tct Ser ttt Phe 150 caa G n aat Asn at a l Ie ctt Leu ct g Leu ggt Sy gct Al a cat Hi s cat Hi s aca Thr at g Met acg Thr 120 caa G n gaa G u gac Asp acg Thr agg Ar g 200 gat Asp act Thr ggt Sy gca Al a cga Ar g cgc Ar g gga Sy at t SIle ttt Phe at t SIle ccg Pr o ct g Leu 170 agg Ar g gt c Val gac Asp ct c Leu gct Al a gag G u gga Gy gat Asp 75 cga Ar g gcc Al a cct Pr o gaa G u cat Hi s 155 at t SIle at c SIle ggc G y at g Met gca Al a cga Ar g ctt Leu t ca Ser ct g Leu act Thr gag G u gcc Al a gcc Al a 140 gtt Val gt g Val agt Ser t ca Ser gct Al a 220 ct c Leu aaa Lys gag G u 45 cat Hi s ctt Leu gag G u gct Al a tgc Cys 125 gt c Val ccc Pr o gt g Val ctt Leu ggt G y 205 gag G u ttt Phe gt c Val gca Al a cca Pr o caa G n cag G n ct a Leu 110 cgt Ar g cag G n gt a Val at t SIle cgg Ar g 190 acc Thr ccg Pr o gt g Val 15 gga G y gac Asp aca Thr tct Ser ccg Pr o 95 ctt Leu gt c Val aaa Lys cgg Ar g tct Ser 175 gag G u cga Ar g at t SIle gcg Al a at g let at g let gct Al a gt a Val ct g Leu cca Pr o gac Asp ggg Sy ctt Leu 160 cct Pr o ttg Leu gaa G u caa G n at g Met 1 at t l Ie gcc Al a aaa Lys gct Al a caa G n aaa Lys gct Al a gt c Val cac Hi s 145 cat Hi s aat Asn tcg Ser gca Al a gt g Val 225 gt g Val caa G n gcc Al a gga Gy gaa G u ttg Leu tgg Tr p tct Ser 130 ttg Leu gct Al a cat Hi s gaa G u tta Leu 210 tta Leu gaa G u cct Pr o gaa G u ctt Leu ttt Phe cat Hi s gtt Val 115 gt g Val caa G n cgt Ar g gag act Thr 195 caa G n aac Asn gag G u aat Asn ttg Leu gcg Al a act Thr gtt Val 100 gcg Al a at g let ct a Leu gtg Val tgg Tr p 180 ccg Pr o gaa G u agc Ser gat aat ggg Asp Asn G y Page 107 seqI i st car r ect ed. t xt gct gcg gt a cgt gt t gt t gt t gaa gca ggg gca ggt cct Ala Ala Val Ar g Val Val Val O31u Ala G31y Ala G31y Pro 230 235 240 ggt gaa t ta gcc t tg cgt gat cat ct t gcg ct c ggc agg G31y (31u Leu Ala Leu Ar g Asp Hi s Leu Ala Leu (31y Ar g 250 255 gt g cca t tt gaa ggc agt gga gt t act cgt cct ct t aca Val Pr o Phe O31u G31y Ser G31y Val Thr Ar g Pr o Leu Thr 265 270 agt gga ccc cgc aga t tg ccg at t ct a gcg gga gaa tt a Ser G31y Pr o Ar g Ar g Leu Pr o Ilie Leu Ala 31y O31u Leu 280 285 290 gca t cg aac cac at c t gatt t tgag ccct ggct aa cgg A a Ser Asn Hi s Il e 295 <210> <211> 298 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn gca A a ct g Le u gct A a 275 gt g ValI gt a ct t Val Leu 245 t tg agt Leu Ser 260 gt g t gg Val Tr p t cc at c Ser I Ie 1017 <400> [vb t As pAs n (1y le A a Ly s A a O31n Ly s A a ValI Hi s 145 Hi s As n Ser A a O31u Pr a O31u Le u Ph e Hi s ValI 115 ValI O31n Ar g O31u Thr 1 95 (31n Tr p O31y A a Le u Le u O31u O31y As p As n O31y ValI 1 65 A a Le u Le u Le u Ar g Ar g O31u ValI Ser Ar g Ser 135 li1e O31u Ar g ValI A a 215 O31y A a 40 Hi s Hi s Thr vb t Thr 120 O31n O31u As p Thr Ar g 200 As p Pr o As n Leu O31n A a li1e Pr a Ser O31u Thr 1 05 Ar g ValI Thr Ly s O31y 1 85 O31u Tyr Thr 10 O31y A a Ar g Ar g O31y 90 lie Ph e I Ie Pr a Le u 1 70 Ar g ValI Le u A a O31u O31y As p 75 Ar g A a Pr a O31u Hi s 1 55 le le Oy A a Ar g Le u Ser Le u Thr O31u A a A a 140 ValI ValI Ser Ser Le u Ly s O31u Hi s Le u O31u A a cys 125 ValI Pr a ValI Le u O31y 205 Ph e ValI A a Pr a O31n O31n Le u 110 Ar g O31n ValI I Ie Ar g 1 Thr ValI O31y As p Thr Ser Pr a Le u ValI Ly s Ar g Ser 1 O31u Ar g A a vb t vb t A a ValI Le u Pr a As p O31y Le u 1 Pr a Le u (31u Asp N/t Ala C3u Pro Ilie On 220 Page 108 seqI i st car r ect ed. t xt Val Leu Asn Ser Asn Al aAl aVal Arg Val Val Val Gu Ala(3y Ala 225 230 235 240 O31y Pro Al a Val Leu O31y O31u Leu Al a Leu Ar g As p Hi s Leu Al a Leu 245 250 255 G31y Ar g Leu Leu Ser Val Pr o Phe O31u G31y Ser G31y ValI Thr Ar g Pr o 260 265 270 Leu Thr Al a Val Tr p Ser G31y Pr o Ar g Ar g Leu Pro I Ie Leu Al a G31y 275 280 285 O31u Leu Val Ser Ilie Al a Ser Asn Hi s Ilie 290 295 <210> 91 <211> 1214 <212> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 OIDS <222> (1191) <223> RXA02431 <400> 91 gt g ValI gt g ValI at c I Ie g aa O31u gt t ValI gcc Al a cca Pr a ct t Le u gt g ValI at c I Ie 145 gt g ValI c aa O31n g aa O31u gt g ValI G31y at t I Ie g ac As p gga G31y 115 ac a Thr ct c Le u ccc Pr a gt g ValI tt c Ph e c ag O31n ccc Pr a t ca Ser c aa O31n ggt G31y ggt G31y gca Al a cgt cat at c gt t t ac t cc gca gcc t cg cgc cgg Arg His IlieVal Tyr Ser Ala Ala Ser Arg Arg 10 gaa (31u at g vb t t gg Tr p t cc Ser 70 ggc G31y cat Hi s cct Pr a g at As p acc Thr 150 aaa Lys5 g aa (31u gcg Al a t cg Ser g aa (31u g ac As p gt g ValI ct a Le u 135 at c I Ie cgc Ar g cca Pr a 40 g aa (31u gt t ValI gca Al a tt g Le u ac a Thr 120 gca Al a gga G31y gcc Al a gag (31u g aa (31u ggt G31y aag Lys5 ct t Le u 1 05 ggc G31y gcg Al a at a I Ie gga G31y gct Al a tt a Le u gct Al a cca Pr a 90 g at As p t cc Ser ct a Le u t ca Ser at t I Ie ct c Le u cgc Ar g ggc G31y 75 g at As p cca Pr a aag Lys5 ac c Thr ct g Le u 1 55 gt c ValI gt t ValI gcg Al a t cc Ser ggt G31y ct t Le u ct t Le u c aa O31n 140 t gg Tr p g aa O31u gga O31y g aa O31u ggt O31y gt g ValI cct Pr a gcc Al a 125 aaa Lys5 aac As n cgc Ar g gcc Al a at t I Ie aag Lys5 tt t Ph e gt g ValI 110 t ca Ser g aa O31u ct t Le u ct c Le u ac c Thr aaa Ly s c ag O31n gt c ValI ggc O31y at g vb t gt a ValI gcc Al a ag c Ser cca Pr a g aa O31u at c I Ie gt g ValI gca Al a ggg O31y g aa O31u cga Ar g 1 Page 109 seql i st cor r ect ed. t xt gga atc gac gac G y I e Asp Asp cgc Ar g 165 cct gtg gaa ccc Pro Val G u Pro gcc gaa gca aaa Ala G u Ala Lys cag atc Gn lle 175 t cc caa gag Ser G n G u gat gct gcc Asp Al a Al a 195 acc tat gaa aaa Thr Tyr G u Lys ctc ctc acc agg Leu Leu Thr Ar g caa caa gta Sn G n Val 190 cgg ct c ct c Arg Leu Leu atc att cga tca lie Ile Arg Ser gcc Al a 200 gaa ggc gca cac G u G y Al a Hi s cga Ar g 205 aaa gac Lys Asp 210 gga cgc ggt gcc G y Arg G y Ala aga Ar g 215 act gtc agc gtg Thr Val Ser Val aaa Lys 220 ctg cgg atg gcc Leu Arg IVet Ala gac Asp 225 ttt cgt att gag Phe Arg lie u cgt tcc tac acc Arg Ser Tyr Thr ttg Leu 235 t cc tat gcc acc Ser Tyr Al a Thr gat tac gca act Asp Tyr Ala Thr ctt Leu 245 gag gca aca gca G u Ala Thr Ala cga ctt gcc cgc Arg Leu Ala Arg t ac ccc Tyr Pro 255 gga gaa gt a G y G u Val gaa gaa tcc G u u Ser 275 ccc atc cgc ctt Pro Ile Arg Leu gt c Val 265 gga gt a agt G y Val Ser ttt tct ggt ttg Phe Ser G y Leu 270 cgc caa gac at c Arg n Asp lie ttc ccg gaa ctt Phe Pro G u Leu gac Asp 285 caa caa atc Gn Gn lie atc gt a Ile Val 290 cca cca gca ccc Pro Pro Ala Pro gac Asp 295 acc gat tat gag Thr Asp Tyr G u gta Val 300 ggc gtg caa tcc Sy Val G n Ser tct Ser 305 tct agt tcc gaa Ser Ser Ser G u act caa gtt gaa Thr G n Val G u gcg Al a 315 ccg caa gat gt c Pro G n Asp Val ttg agt atg tgg Leu Ser IVet Trp tgc Cys 325 gca acg caa gat Al a Thr G n Asp gtc tac cac cca gaa tat ggc Val Tyr His Pro G u Tyr G y 330 335 cac ggt tgg His G y Trp gaa acc cgc G u Thr Arg 355 caa ggt gcc ggt G n y Ala G y cac Hi s 345 ggt gtt gta tca G y Val Val Ser gta cgt ttt Val Arg Phe 350 t cc atg gat Ser IVet Asp 912 960 1008 1056 1104 1152 1201 agc acc aca aaa Ser Thr Thr Lys cga act aaa agt Arg Thr Lys Ser ttt Phe 365 gac ccg Asp Pro 370 gac ctc acc ccg Asp Leu Thr Pro gca Al a 375 gac cct cta gat Asp Pro Leu Asp ttg gat tgg gct Leu Asp Trp Ala gac Asp 385 tgg ttt gct gaa Trp Phe Ala G u ggt gaa acg ggg G y G u Thr G y gat gac gaa Asp Asp G u 395 tagggt t t ca tcgggtttcg ggg 1214 <210> 92 <211> 397 <212> PRT <213> Cor ynebact eri um gl ut am cum Page 110 seqi i st car r ect ed. t xt <400> 92 Val Val Val Thr ValI I Ie O31u ValI A a Pr o Le u ValI I Ie 145 O31y Ser As p Ly s As p 225 As p O31y Ou le Ser 305 Le u Hi s O31n O31u ValI O31y I Ie As p O31y 115 Thr Le u As p O31u A a 1 95 O31y Ar g A a ValI Ser 275 Pr o Ser vb t Tr p Pr o ValI Ph e O31n Pr o Ser O31n O31y (31y A a Ar g 1 65 Thr I Ie G31y O31u Le u 245 Pr a O31n A a O31u cys 325 O31n Arg His IlieVal O31u vb t Tr p Ser 70 O31y Hi s Pr a As p Thr 150 Pr a Tyr Ar g A a Ser 230 O31u I Ie As p Pr a Ser 310 A a O31y Lys5 O31u A a Ser O31u As p ValI Le u 135 li1e ValI O31u Ser Ar g 215 Ar g A a Ar g I Ie As p 295 Thr Thr A a Ar g Pr a 40 O31u ValI A a Le u Thr 120 A a O31y O31u Lys5 A a 200 Thr Ser Thr Le u Le u 280 Thr O31n O31n O31y A a O31u O31u O31y Lys5 Le u 1 05 O31y A a I Ie Pr a As p 1 85 O31u ValI Tyr A a ValI 265 Ph e As p ValI As p Hi s 345 Tyr 10 O31y A a Le u A a Pr a 90 As p Ser Le u Ser Ar g 1 70 Le u O31y Ser Thr Ph e 250 O31y Pr a Tyr O31u ValI 330 O31y Se r A a A a Se r I Ie Le u Ar g O31y 75 As p Pr a Lys5 Thr Le u 1 55 A a Le u A a ValI Le u 235 Ar g ValI O31u O31u A a 315 Tyr ValI O31u O31y O31u O31y ValI Pr a A a 125 Ly s As n A a Ar g Ar g 205 Le u Tyr A a Ph e As p 285 O31y O31n Pr a Ser Ar g A a I Ie Lys5 Ph e ValI 110 Ser G u Le u Ly s O3n 1 Ar g Ar g A a Ar g Ser 270 O3n ValI As p O3u ValI 350 Ar g Ar g Leu Ser Thr Pr o Lys 03 u O31n I Ie Val Val O3 y A a Vb t (1y Val (31u Ala Ar g 1 O31n I Ie 1 O3 n Val Leu Leu [vb t A a Thr As p 240 Ty r Pr o 255 O3 y Leu O31n I Ie O3 n Ser Val Ala 320 Ty r 03 y 335 Ar g Phe Ou Thr Arg Ser Thr Thr Lys Oy Arg Thr Lys Ser Phe Ser l'bt Asp Page 111 seql i st cor r ect ed. t xt 355 360 365 Asp Pro Asp Leu Thr Pro Ala Asp Pro Leu Asp Ser Leu Asp Trp Ala 370 375 380 Asp Trp Phe Al a u Asn 3 y u Thr y Asp Asp u 385 390 395 <210> 93 <211> 558 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(535) <223> RXA02446 <400> 93 caggaaatct tgctgtctaa tgcacaacgg aaaactgctc acggacgaac tagttgtagc gagcgtattc tttgtgttct ctcacgacag gaatactgct atg gcg atc gag tcc IVet Ala lIe Gu Ser at c SIle cat Hi s aag Lys gcg Al a cgc Ar g agc Ser gaa G u gat Asp at c l Ie t ac Tyr cgc Ar g ttg Leu ggt Gy cgc Ar g ggg Gy tcg Ser gcg Al a 120 gt a Val agt Ser aca Thr gga Gy gca Al a at c SIle aac Asn cct Pr o cag G n gaa G u gat Asp t cc Ser gcc Al a act Thr 75 ggc Gy caa G n gt g Val gca Al a ggt Gy ggt Gy tgt Cys ctt Leu gct Al a ttg Leu tgc Cys ct c Leu gcg Al a gaa G u 45 ttc Phe gaa G u ggt Gy cca Pr o ccg Pr o 125 t ca Ser ct c Leu ggc Gy cac Hi s gac Asp gga Gy caa G n 110 t at Tyr acc Thr 15 gaa G u acc Thr tct Ser aca Thr ttt Phe 95 gca Al a t cc Ser ggc Gy ttt Phe aat Asn gcc Al a gcg Al a 80 gag G u gaa G u aac Asn 1 agt Ser gaa G u cca Pr o at g Met gtt Val at t SIle gcc Al a gcc Al a gct Al a 145 ggc Gy at g let gaa G u cac Hi s ggt Gy gcc Al a cag G n aca Thr 130 cgg Ar g aca Thr cag G n tct Ser gcc Al a gt a Val gaa G u 115 cga Ar g ct g Leu ccg Pr o ct g Leu gt c Val cga Ar g gaa G u 100 ctt Leu ggc Gy ggg Gy cca Pr o ttc Phe gca Al a gtt Val ct c Leu gct Al a aat Asn gtg tca gt c Val Ser Val 140 atc gac gaa gag I e Asp G u G u taaaaccaca ggattaacaa aac <210> 94 <211> 145 <212> PRT Page 112 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <400> 94 Met Ala lle Gu Ser Ile Ala Tyr Thr Ser u Ala Le 1 5 10 Ser G y Arg Leu G y His Val Arg Ser Thr Asp G y Al G u Met Thr Pro Pro Lys Ala Leu Gy y Ser Gy G 40 4 Pro G u G n Leu Phe Al a Val y Tyr Ala Ala Cys Ph 55 IVet His Ser Val Ala Arg Ser Arg Lys Ile Thr Leu G 70 Val y Ala Arg Val Ser I e y Pro Asn y Ala G 90 Ile Ala Val G u Leu G u Val Ser Ile Pro G n Leu Pr 100 105 Ala n G u Leu Ala Asp Ala Ala His G n Val Cys Pr 115 120 12 Ala Thr Arg y Asn I e Ser Val Thr Val Ser Val II 130 135 140 Al a 145 <210> <211> 1206 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1183) <223> RXA02861 <400> ccctcgatta ttacaatccg gctacttcgg aagcgtttgt ggccgg gaaggtatta atacttcttt aagggtcgga ggattttcgt atg tc u a u e u y 0 o 0 e Thr G u Thr Ser Thr Phe Al a Ser G u attt ttctatcctg t act aga aca acg Thr aag Lys ttt Phe aac Asn ccg caa G n gaa G u cat Hi s ttg Leu gcc cgt Ar g ggc G y at g Met gcg Al a ctt acc Thr gt g Val at g Met at t l Ie ttc gaa G u aaa Lys gaa G u t at Tyr at t tac ggc Tyr G y 15 ctc cgg Leu Arg cgt ttc Arg Phe ttc tcc Phe Ser gca ggt Page 113 Iet Ser Thr Arg Thr 1 atc gaa cgc gtc aac l e G u Arg Val Asn gac aag cac gac tgg Asp Lys His Asp Trp ggc gca aaa ggt ggc G y Ala Lys G y G y gtg ctg tcg atc ttc Val Leu Ser Ile Phe gtc atc ctt gcc gga seqi i st car r ect ed. t xt ProiieAia bt Leu VaiPhe Oy IeAia3y Val1 75 80 e Leu A a 03 y aac As n g aa O31u gcg A a t ct Ser t gg Tr p 1 50 g ac As p ggt G31y tt t Ph e gt c ValI ct g Le u 230 c ag O31n gga G31y t tc Ph e at c I Ie cgt Ar g 310 cat Hi s g aa O31u gag O31u cgt Ar g 120 ct g Le u at t I Ie gt c ValI ac g Thr ggc G31y 200 gca A a tt c Ph e gca A a ct g Le u ccg Pr o 280 at g vb t gcg A a at t I Ie ct c Le u ggt G31y gct A a t gg Tr p cca Pr o ct g Le u 1 70 ct c Le u ggg G31y gt t ValI ct g Le u ct t Le u 250 gct A a at c I Ie t gc cys gt t ValI cca Pr o 330 aca Thr aac As n gt g ValI at g vb t act Thr 155 at c I Ie ggt G31y gag O31u ggt G31y cca Pr o 235 ggc G31y t ca Ser ggc G31y t ct Ser cca Pr a 315 ggt G31y g at As p ac c Thr tt g Le u gcg A a 140 g aa O31u gt c ValI gct A a at t I Ie gt c ValI 220 cgt Ar g gca A a aat As n at c I Ie gcg A a 300 gca A a g aa (31u at t I Ie gt c ValI ggc G31y 125 aac As n ggc G31y tt g Le u t cc Ser ccg Pr a 205 tt g Le u ac c Thr at c I Ie gca A a at g vb t 285 t gg Tr p cca Pr a g aa O31u c aa O31n aac As n 110 at t I Ie ct g Le u aac As n ct g Le u gga G31y 1 90 ggc G31y gct A a aaa Ly s ggt G31y Le u 270 gt t ValI gct A a gag O31u gt c ValI cga Ar g at c I Ie ggt G31y Ph e at t I Ie 1 60 gcc A a ac c Thr ag c Ser tt c Ph e ccc Pr a 240 gag O31u aac As n ct g Le u ac c Thr gcg A a 320 c aa O31n at c I Ie at t I Ie gt a ValI gga G31y 145 c aa O31n at g vb t aaa Ly s t ac Tyr ct g Le u 225 at g vb t gt g ValI ccc Pr a t at Tyr agt Ser 305 at c I Ie t ct Ser aac As n g at As p act Thr 130 gt t ValI aag Ly s ggc O31y aac As n at c I Ie 210 gt g ValI aaa Ly s gt c ValI gcg A a tt g Le u 290 g aa O31u at t I Ie gct A a g at As p t cc Ser 115 gcc A a t cc Ser aag Ly s gt a ValI ct g Le u 1 ac c Thr tt c Ph e ccg Pr a aag Ly s ggt O31y 275 at c I Ie g aa O31u Ar g cga Ar g gct A a 1 00 gcg A a ct g Le u cgc Ar g ct c Le u gcc A a 1 ct g Le u t gg Tr p at g vb t ggt O31y c ag O31n 260 gca A a t gg Tr p gcg A a gt t ValI aaa Ly s 340 tt a Le u at t I Ie t gg Tr p at g vb t ac c Thr 1 tt c Ph e g ac As p gt g ValI t gg Tr p ct t Le u 245 gt t ValI gca A a cgc Ar g tt g Le u cgc Ar g 325 gt g ValI 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 ggc at t gga gt g gcc gt g ggt gcc gcg act gcg ggt gct t tt gcg ct g Page 114 seqI i st car r ect ed. t xt Gy IleOy Val AlaVal Oy AlaAlaThr AlaOy Ala Phe Ala Leu 345 350 355 ttg cgt aaa aaa t agt tt t tat t aagggcat t ccc Leu Arg Lys Lys 360 <210> 96 <211> 361 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1206 <400> 96 I'bt Ser Thr li1e G31u Ar g As p Lys Hi s a3 y Al a Lys Val Leu Ser Val Ilie Leu Il e Asn Asp Il e Asp Ser 115 Val Thr Al a 130 G31y Val Ser 145 a3 n Lys Lys [vb t G31y Val Lys Asn Leu 1 95 Tyr Ilie Thr 210 Leu Val Phe 225 I'b t Lys Pr o Val Val Lys Pr o Al a G31y 275 Ar g Thr Val As n As p Tr p G31y (1y I I e Phe Al a G3 y Al a Leu 1 00 Al a I I e Leu Trp Ar g [Vb t Leu Thr 1 65 Al a Phe 1 80 Leu Asp Tr p Val I'b t Tr p G3 y Leu 245 a3 n Val 260 Al a Al a Thr Ly s Ph e As n Pr o 70 As n a31u Al a Ser Tr p 150 As p a31y Ph e ValI Le u 230 a31n a31y Ph e Pr o As p As p a31n 55 li1e Pr o a31y a31n a31y 135 Al a Le u I Ie ValI Al a 215 li1e Al a Ser a31y a31n a31u Hi s 40 Le u Al a a31u a31u Ar g 120 Le u I Ie ValI Thr a31y 200 Al a Ph e Al a Le u Pr o 280 As p Pr a Le u Ser vb t ValI I Ie 1 a31y a31y As p Al a Al a 1 Le u Le u Ser Le u Le u 265 li1e Ar g 10 a31y vb t Al a Le u Le u 90 a31y Al a Tr p Pr a Le u 1 70 Le u a31y ValI Le u Le u 250 Al a Tyr Le u Ar g a31y ValI Thr As n ValI vb t Thr 1 li1e a31y a31u a31y Pr a 235 a31y Ser Thr ValI vb t I Ie Ph e As p Thr Le u Al a 140 a31u ValI Al a I Ie ValI 220 Ar g Al a As n As p As p As n Thr a31y I Ie ValI a31y 125 As n a31y Le u Ser Pr a 205 Le u Thr I Ie Al a Tyr Leu Ar g Ph e Al a As n a31y a31y Ar g Ph e Leu 1 Leu I Ie As n ValI Ph e 255 Ser lieay Ilie I'bt Val Val Leu 285 Page 115 Tyr Leu lie Trp 290 Ser G u G u Ala 305 lie Ile Arg Val Ser Ala Arg Lys 340 G y Al a Phe Al a 355 seql i st cor r ect ed. t xt Arg Ile Leu IVet Tyr Cys Ser Ala Trp Ala Ala Thr 295 300 Leu Arg Leu Ala Thr Val Pro Ala Pro G u Pro Ala 310 315 320 Arg His G u lie Asp Pro G y G u G u Val Ser G n 325 330 335 Val y Ile G y Val Ala Val G y Ala Ala Thr Ala 345 350 Leu Leu Arg Lys Lys 360 <210> 97 <211> 753 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(730) <223> RXA00981 <400> 97 aaccaatggc tgggtactga tgtggtgatc agtgcccagt ggatagaagt acccccagtc cagaatgaag gtcaccacca ttcttctttc tactagtgtc cca Pr o aac Asn at t SIle cag G n aca Thr t cc Ser at c SIle acc Thr aac Asn ccc Pr o cgc Ar g gag G u cac Hi s gaa G u gca Al a tgc Cys caa G n 120 aag Lys aat Asn gaa G u ct g Leu t ac Tyr at c SIle at c l Ie ttc Phe gac Asp aac Asn ct c Leu tta Leu acc Thr gca Al a gt c Val 75 aaa Lys ct c Leu gt c Val ggc G y ct g Leu acg Thr aag Lys cct Pr o aaa Lys gac Asp aaa Lys acg Thr t ac Tyr 140 gcc Al a ttc Phe aac Asn gag G u gt c Val gt g Val gcc Al a 110 at c SIle agt Ser aga Ar g ct c Leu ct g Leu gt a Val cga Ar g gat Asp gca Al a gcc Al a atg t ca Vet Ser 1 att cag Ile G n aac t ac Asn Tyr aaa act Lys Thr tct tca Ser Ser aaa gga Lys G y atc gca I e Al a atc gcc I e Al a aaa gac Lys Asp 130 at g Met caa G n gaa G u cga Ar g gac Asp ggc G y gat Asp 115 t ac Tyr at c I e ttt Phe ttc Phe ttg Leu gag G u att SIe 100 at g Met at c SIe gag aat ttg G u Asn Leu ttg cac ctt at c ctc caa gtg Leu His Leu Ile Leu G n Val 145 Page 116 seqI i st car r ect ed. t xt cct gt c t tc ct g t ct aac tcc gtg gaa aag gt c aat gt t Pr o Val Phe Leu Ser Asn Ser Val 03 u Lys Val Asn Val 150 155 160 at c cgc acc at t gcc at g gac t tc t gg gca agc ct c gag Ilie Ar g Thr Ilie Ala rvb t Asp Phe Tr p Ala Ser Leu O31u 170 175 t ac t ac aaa t tt gaa caa gaa gt t cct cag t ca at c ct t Tyr Tyr Lys Phe 31u O31n O31u Val Pr o O31n Ser Ilie Leu 185 190 agt gaa gat gga aag aat cca cgg gga agt gaa gt c act Ser O31u Asp O31y Lys Asn Pr o Ar g O31y Ser O31u Val Thr 200 205 210 gt tgaaacca ct g <210> 98 <211> 210 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn gaa gt c cag O31uVal O31n 1 cac aaa atc Hi s Lys IlIe 1 gat gag ct c Asp 03 u Leu 1 t aaacct cca <400> 98 I'bt Ser 03 u Asn li1e O31n I'b t IIe Asn Tyr 03 n Phe Lys Thr 03 u Phe Ser Ser Ar g Leu Lys 03 y As p 03 u I e A a 03 y I I e 1 00 I I e A a As p Nib t 115 Lys As p Ty r I Ie 130 Leu I I e Leu 03 n 145 As n Val O31u Val Leu 03 u Hi s Lys 1 80 Le u Ly s G31y Ser Ly s Le u Ar g Le u A a ValI O31n 1 65 li1e Pro Ala Pro Ou Ph e As p Le u Pr o Le u ValI As n Pr o 135 ValI Ar g Tyr Ar g O31u 40 Hi s O31u A a cys O31n 120 Ly s Ph e Thr Ly s As n 25 li1e O31u Ser A a Ser 1 05 Ly s Pr o Le u I Ie Ph e 1 85 As n 10 O31u Le u Tyr I Ie I Ie 90 Ph e As p As n Ser A a 1 70 O31u Le u Le u Thr A a ValI 75 Ly s Le u ValI O31y As n 1 55 vb t O31n As p O31y I Ie li1e Ly s Thr As p ValI 125 Ly s ValI Ph e ValI Ar g 205 A a Ph e As n O31u ValI ValI A a 110 I Ie Ser O31u Tr p Pr a 1 O31u vb t I Ie Hi s I Ie Ph e Tyr O31u Le u Ly s A a 1 O31n Ar g Le u Le u ValI Ar g As p A a A a Hi s ValI 1 Ser Ser Il e Leu Asp 1 95 Val Thr 210 0 u Leu Ser 0 u Gy Lys Asn Pro 03 y Se r 03 u Page 117 seqI i st car r ect ed. t xt <210O> <211 <21 2> <21 3> <220> <221 <222> <223> 99 1 098 DNA Cor ynebact er i urn CIDS (1075) RXN0 0786 gi ut ani cumn <400> 99 t cct t ct t cc accagct aaa agt gaat tac cccgcct t tg t cggggt ggt t gcat tccca gt ct aggt gt t t agcct caa cgt t ggat ac gct gggaggc at g b t 1 agc t ca cct Ser Ser Pr o at c agc ccc gaa I Ie Ser Pro G31u ac c Thr aaa acc gga aag Lys Thr G3 y Lys atc ctg ctt gca Il e Leu Leu A a gcc cct Ala Pr o cgc gga t ac Ar g G31y Tyr gcg ct c gag A a Leu G3 u gcc ggc gta gac Ala G31y ValI Asp cgt Ar g gca gt g gaa acc Ala Val 0 u Thr gt c gag cgc Val G31u Ar g gaa at c gt g a3 u IlIe Val gaa t ac ggc gcc G3 u Tyr G3 y A a cca Pr o 45 at t t at gt c cgt Ilie Tyr Val Ar g aaa Ly s cac aac Hi s Asn cgt t ac gt t gt g Ar g Tyr Val Val g ac Asp acc ct g gca gaa Thr Leu A a a3 u ggc gcg at t tt t a3 y A a Il e Phe gt c ValI aac gaa gca t ct Asn a3 u A a Ser gaa a31u 75 gca cca gaa ggt Ala Pr o 31u a31y gcc A a aac at g gt g tt c Asn I'b t Val Phe gca cac ggc gt g A a Hi s a3 y Val ag c Ser cca atg gtc cac Pr o I'bt Val Hi s g aa a31u 95 gaa gct gca gct a3 u A a A a A a aaa aac Lys Asn 1 00 at c aag gct Ile Lys A a gaa gt c cag a31uVal a31n 120 gac gcg gcc tgc Asp A a A a Cys ccg Pr o 110 ct g gt c acc aaa Leu Val Thr Lys gt g cac aag Val Hi s Lys 115 t tc at c ggt Phe Il e a3 y cgc t tt gat aag Ar g Phe Asp Lys gga t tc cac at t a3 y Phe Hi s Il e ct c Le u 130 cac gaa Hi s a3 u 135 ggc cat gaa gaa a3 y Hi s a3 u a3 gag ggc acc at g a3 u a3 y Thr I'b t cat tcc gtt gag Hi s Ser Val a u aaa 1 50 acc cac ct g gt t Thr Hi s Leu Val ggc gt t gct ggc a31y Val Ala a31y at t Ilie 1 gcc acc ct g cct Ala Thr Leu Pr o t tc t ta aac gat Phe Leu Asn Asp g aa a31u 1 70 cca aac ct g at c Pro Asn Leu Ilie ct g t ct cag acc Leu Ser a3 n Thr acg ct t Thr Leu 1 t ct gt g gac Ser Val Asp acc at g gag at c Thr I'b t a31u Ilie gt c ValI 1 90 cgc gag ct g aag Ar g a31u Leu Lys gt g aag tt c Val Lys Phe 1 cct cag ct g cag gat cca ccg tca gat Pro a31n Leu a31n Asp Pr o Pr o Ser Asp gat at t t gc tac gcc acg cag Asp Il e Cys Tyr A a Thr a3 n Page 118 seqI i st car r ect ed. t xt 200 205 210 aac cgc cag gt t gcc gt c aag gct at c gct gag cgc t gc As nAr g 1n Val Ala Val Lys Ala IlIe Ala 1u Ar g ys 215 220 225 at t gt ggt cggt t cc cgc aac tcc tcc aac tcg gtt cgt I Ie Val Val G31y Ser Ar g As n Ser Ser As n Ser Val Ar g 230 235 240 gt c gct aag caa aac ggt gcc gat aac gcc t ac ct g gt g Val Ala Lys O31n Asn G31y Ala Asp Asn Ala Tyr Leu Val 250 255 cgc gaa atc gac cca gca tgg ttc gaa ggc gta gag acc Ar g O31u Ilie Asp Pr o Ala Tr p Phe O31u G31y Val O31u Thr 265 270 t cc t cc ggc gct t cc gt g cct gag at c ct c gt c cag ggc Ser Ser G31y Ala Ser Val Pr o O31u Ilie Leu Val O31n O31y 280 285 290 cgc ct ggct gagt t cggc tac gac gac gt cgag gaa gt c Ar g Leu Ala O31u Phe O31y Tyr Asp Asp Val O31u O31u Val 295 300 305 gct gag aag at t gt t t tc gcg ct g cct cgc gt g ct g cgc Ala O31u Lys Ilie Val Phe Ala Leu Pr o Ar g Val Leu Ar g 310 315 320 t aat tgcaag aat gaaaaat ccc <210> 100 <211> 325 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn ct g Le u gt t ValI t ac Tyr 260 ggt G31y at t I Ie t cc Ser aag Ly s 787 835 883 931 979 1 027 1 075 1 098 <400> 100 I'bt Ser Ser Pro Val lieSer Pro Ou Thr Lys Thr Gy Lys Le u O31u Ar g Ly s As n A a Thr I Ie (31y 145 A a ValI O31u A a ValI A a ValI 115 Ph e Ser Pr o Ar g ValI Ph e Ser As n Ly s G31y (31u G31y Le u As n As n Hi s Ly s ValI O31u 135 Thr Tyr O31u 40 Ar g O31u G31y A a O31n 120 G31y Hi s cys (31u Tyr A a ValI I Ie 1 Ar g Hi s Le u 10 A a 03 y Val Ty r 03 y A a Val Val As p Ser 03 u A a 75 Ser Pr o I'b t 90 As p A a A a Phe As p Lys O3 u 03 u Val 140 Val As p (1y 1 55 Page 119 As p Pr o Thr Pr o ValI cys O31n 125 O31u ValI Lys I I e A a Val Ty r Val A a 03 u O3 y A a O31u (1u Leu Val Phe Hi s Thr I'b t G31y I Ie 1 Al a Ser Leu Cys Ar g 225 Val Leu G u G n G u 305 Leu Leu Thr Val 195 Al a G u Leu Asp SIle 275 Val Thr Hi s G u 165 Leu Phe G n Met G u 245 Al a SIle G u Al a Asn 325 Leu Val G n Ar g 215 Val Al a G u Ser Leu 295 G u seql i st cor r ect ed. t xt Asn Asp G u Pro Asn Leu 170 Asp u Thr IVet u lie 185 Leu G n Asp Pro Pro Ser 200 205 G n Val Ala Val Lys Ala 220 Val y Ser Arg Asn Ser 235 Lys G n Asn G y Al a Asp 250 Ile Asp Pro Ala Trp Phe 265 G y Ala Ser Val Pro G u 280 285 Al a G u Phe G y Tyr Asp 300 Lys lie Val Phe Ala Leu 315 Tr p 175 Ar g Asp Al a Asn Al a 255 Sy Leu Val Ar g <210> 101 <211> 1131 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1108) <223> RXS01027 <400> 101 aatagatgga agtagttttt cattcactta tgtgcgcgtt gaactgtgtg caccacaacg cggaaggtga atcgcaccca aat Asn aac Asn cgc Ar g aac Asn cct Pr o cgc Ar g act Thr gac Asp gag G u caa G n gca Al a at c SIle gt g Val cag G n gca Al a gag G u gac Asp at c SIle ggt G y gt c Val aaa Lys ccg Pr o gca Al a 45 gct Al a gac Asp gcg Al a gt a Val gt c Val caa gat Sn Asp 15 cgt ccc Arg Pro ctg tgg Leu Trp atc cac Ile Hi s Page 12C tttaatctgg tttctaccaa atg gca aat aag aac IVet Ala Asn Lys Asn 1 tca gcc atg ctg atc Ser Ala IVet Leu Ile act gag gaa ttc acc Thr G u G u Phe Thr cgc ggc gac atc acc Arg y Asp Ile Thr cgc ccg cac tat gat Arg Pro His Tyr Asp NO seql i st cor r ect ed. t xt gac tgg tcc ctg gcc aag ggc aaa gtc gat ccc ggc gag tct att ccg 355 Asp Trp Ser Leu Ala Lys y Lys Val Asp Pro Gy u Ser lie Pro 75 80 aca acc gcg gcc cgt gaa atc ctt gaa gaa act ggc tac gac atc cgt 403 d) Thr Thr Ala Ala Arg Gu Ile Leu Gu u Thr y Tyr Asp lie Arg 90 95 100 C ctg ggc aag ctg atc ggc aag gtt act tac cct gtg ctc gac cga acc 451 Leu y Lys Leu Ile G y Lys Val Thr Tyr Pro Val Leu Asp Arg Thr 105 110 115 aaa gtg gtc tac tac tgg act gcc cag gtt ctt ggt gga gag ttt gtc 499 0 Lys Val Val Tyr Tyr Trp Thr Ala G n Val Leu Gy Gy G u Phe Val 00 120 125 130 0 0 ccc aac gat gaa gtt gat gaa atc cgt tgg ctg tct gtt gat gaa gca 547 Cl Pro Asn Asp u Val Asp u Ile Arg Trp Leu Ser Val Asp u Ala 135 140 145 Stgc gag ttg ctc agc tac caa gta gat acc gaa gtt ctg gcc aag gca 595 C Cys G u Leu Leu Ser Tyr G n Val Asp Thr G u Val Leu Ala Lys Ala 150 155 160 165 gca aag cgt ttc cgc act cct tcc acc act cgg gtg ctg tat gtt cgc 643 Ala Lys Arg Phe Arg Thr Pro Ser Thr Thr Arg Val Leu Tyr Val Arg 170 175 180 cat gct cat gca cat ggt cgc caa acc tgg ggt ggc gac gac aat aag 691 His Al a His Al a His G y Arg G n Thr Trp G y G y Asp Asp Asn Lys 185 190 195 cgc cca ttg gac aaa aag ggg cgt cga caa gca gaa atg ctc gta ccc 739 Arg Pro Leu Asp Lys Lys G y Arg Arg G n Ala G u Iet Leu Val Pro 200 205 210 atg ttg ttg ccc ttc aaa ccc acc gca att tac tcg gcg gtg ccc gat 787 IVet Leu Leu Pro Phe Lys Pro Thr Ala lle Tyr Ser Ala Val Pro Asp 215 220 225 cgc tgc caa gcc acc gcg ctc ccc ctt gcc gat gag ctc ggc ctc gac 835 Arg Cys G n Ala Thr Al a Leu Pro Leu Ala Asp G u Leu y Leu Asp 230 235 240 245 gtg tcc gtc aac cga ctg ttc ggc gac gac gcc tgg gaa acc gat ccc 883 Val Ser Val Asn Arg Leu Phe G y Asp Asp Al a Trp G u Thr Asp Pro 250 255 260 gag gcc tgc aag aag cgc ttc acc gac gtg gtc gcg caa ggt ggc gtg 931 G u Ala Cys Lys Lys Arg Phe Thr Asp Val Val Ala G n y y Val 265 270 275 ccg atg atc gtt ggg cag ggc gac atc att ccg gaa atg atc aaa tgg 979 Pro IVet Ile Val G y Gn y Asp lie lle Pro u IVet lie Lys Trp 280 285 290 ttc tcc gag aac ggc acc ctc cct atc gat gag aag atc aag gcg aaa 1027 Phe Ser G u Asn G y Thr Leu Pro Ile Asp G u Lys Ile Lys Al a Lys 295 300 305 aag ggc agc gtg tgg gtg ttg agc ttt cac gac ggt gtg ttc acc ggc 1075 Lys G y Ser Val Trp Val Leu Ser Phe His Asp G y Val Phe Thr G y 310 315 320 325 gct gat tac ctg gcg agt tcc ctg ccg gtt aaa taggagcgcg tttaaggcct 1128 Ala Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys 330 335 Page 121 seql i st cor r ect ed. t xt cca <210> 102 <211> 336 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1131 <400> 102 Mvt Ala A 1 Ser Al a N Thr G u G Arg G y A Arg Pro H G y Gu S G y Tyr A Val Leu A 1 G y G y G 130 Ser Val A 145 Val Leu A Val Leu T Sy Asp A 1 G u lMt L 210 Ser Al a V 225 G u Leu G Trp Gu T Al a G n G 2 Su et I 290 Lys I I e L 305 sn Lys Bt Leu lu Phe sp I e is Tyr er Ile sp I e 100 sp Arg 15 lu Phe sp G u I a Lys yr Val 180 sp Asn 95 eu Val al Pro Sy Leu hr Asp 260 y G y 75 l e Lys ys Al a Asn 5 I le Thr Thr Asp Pr o Ar g Thr Val Al a Al a 165 Ar g Lys Pr o Asp Asp 245 Pr o Val Tr p Lys Asn Lys Pro Sy Pr o Pr o Tr p Thr Sy Val Asn 135 G u Lys Al a Pr o Leu 215 Cys Ser Al a Met Ser 295 Gy Ar g Thr 40 Asp Ser Al a Lys Val 120 Asp Leu Ar g Hi s Leu 200 Leu G n Val Cys SIle 280 G u Ser His G u Val 10 Leu G n G n 25 Leu Al a Al a Ser Ile G u Leu Ala Lys Al a Ar g G u 90 Leu I e G y 105 Tyr Tyr Trp G u Val Asp Leu Ser Tyr 155 Phe Arg Thr 170 Al a Hi s G y 185 Asp Lys Lys Pro Phe Lys Al a Thr Al a 235 Asn Arg Leu 250 Lys Lys Arg 265 Val y n Asn G y Thr Val Trp Val 315 Lys Pr o Al a Al a Lys Leu Val Al a 125 I le Val Ser G n Ar g 205 Thr Pr o Sy Thr Asp 285 Pr o Asp Al a Val Val Val G u Thr 110 G n Ar g Asp Thr Thr 190 Ar g Al a Leu Asp Asp 270 I e I e G n Ar g Leu SIe Asp G u Tyr Val Tr p Thr Thr 175 Tr p G n SIe Al a Asp 255 Val SIe Asp Leu Ser Phe His Page 122 seql i st cor r ect ed. t xt Sy Val Phe Thr y Al a Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys 325 330 335 <210> 103 <211> 651 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(628) <223> RXS01528 <400> 103 cacccaaacc caaacctctc agtcgaataa gcagaagtct gggtcgtagg tctccaacca ggaggcgttc caacacgagg caggacaacc gcaggggtaa cag G n gt g Val ct g Leu cgt Ar g aag Lys ggt Gy gaa G u gtt Val tgg Tr p cgg Ar g 150 t cc Ser t ca Ser aag Lys ttg Leu gcg Al a gt g Val aag Lys ggc Gy 120 ccg Pr o ttg Leu ttg Leu ttg Leu t at Tyr tcg Ser gct Al a act Thr at c SIle ttg Leu aat Asn agg Ar g gtt Val gct Al a gtt Val at g Met gag G u 75 gag G u cat Hi s aat Asn cag G n cag G n 155 act t ct gat gag Thr Ser Asp G u 15 gag gcg gtc aac Su Ala Val Asn gcg ttg att ggt Al a Leu Ile G y 45 ccg aag ggc cat Pro Lys G y Hi s cgt gag gtg tgg Arg G u Val Trp ttg ggt gtg att Leu y Val lie 95 aag acg gtg cat Lys Thr Val Hi s 110 gat gag gat cca Asp G u Asp Pro 125 ttg att gag cat Leu Ile G u Hi s 140 gcg cat gat ttg Ala His Asp Leu agg tcc acc cca Arg Ser Thr Pro 175 act Thr gct Al a cgc Ar g gtt Val gag G u 80 gat Asp cat Hi s gaa G u ttg Leu ttg Leu 160 agg Ar g gtg aat cag gcg tgg Val Asn G n Ala Trp 1 tcc gca ggt ggt ctc Ser Ala G y G y Leu aac aat gag gtt gat Asn Asn G u Val Asp ctt gat cgt cgt ggt Leu Asp Arg Arg y gag cct ggt gag gat G u Pro G y G u Asp gag acc ggc atc cac G u Thr G y lie Hi s tat tgg ttc gtt tcg Tyr Trp Phe Val Ser 100 cat ttg ttg cgt tat His Leu Leu Arg Tyr 115 gtc act gag gtg gcg Val Thr G u Val Ala 130 gct ttt gcg gat gag Ala Phe Ala Asp G u 145 cct gag ttt gct ttg Pro G u Phe Ala Leu 165 aag gaa aag gcg Lys G u Lys Ala gag gga 1 u y 170 tgattccgaa ccccaacccg 648 aac Page 123 seql i st cor r ect ed. t xt <210> 104 <211> 176 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 104 Val Asn Gn Ala 1 Ser Asn Leu G u G u Tyr Hi s Val Al a 145 Pr o Gy G u Ar g Gy Gy Phe Leu 115 G u Al a Phe Tr p 5 Leu Asp Gy Asp Hi s Ser Tyr Al a G u Leu 165 Val Ser Leu Al a G u Gy Asp l Ie 135 Lys G u Ser Lys 40 Leu Al a Val Lys Gy 120 Pr o Leu Lys Sn G n Ser Arg Gy I le Tr p Thr Phe Ar g 105 Asp Al a Al a Al a Leu 10 Leu Tyr Ser Al a Thr 90 I e Leu Asn Ar g G u 170 Val Al a Val Met G u G u Hi s Asn G n G n 155 Gy Thr G u Al a Pr o Ar g Leu Lys Asp Leu 140 Al a Ar g Ser Al a Leu Lys G u Gy Thr G u 125 I le Hi s Ser G u Asn Gy Hi s Tr p SIe Hi s Pr o Hi s Leu Pr o 175 <210> 105 <211> 509 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> RXS01716 <400> 105 gaa gtc act cct gag gga ttc aaa gag G u Val Thr Pro G u G y Phe Lys G u 1 5 gtt cgc ctg ggc aaa ggc gtc gac gcc Val Arg Leu y Lys G y Val Asp Ala 25 gca atc gag cgc act cgt gtc gct ttg Ala lie G u Arg Thr Arg Val Ala Leu 40 gaa acc cat ggg gta gag gcc gta cga G u Thr His G y Val G u Ala Val Arg 55 atc acc cgt gaa aac acc atc Ile Thr Arg u Asn Thr Ile 10 acc ggt cag cta gac ccc gag Thr Gy n Leu Asp Pro u gaa aac tac gtt gaa ctc atg G u Asn Tyr Val G u Leu IVet atg gtt gcc acc tcc gca acc Met Val Ala Thr Ser Ala Thr Page 124 cgc Ar g ct g Le u gag (31u g ac As p t tc Ph e c aa O31n 145 ccc Pr o seqI i st car r ect ed. t xt gcg tcc aac cgc gat gaa ttc ttt t cg at g acc Ala Se r Asn Ar g Asp O31u Phe Phe Ser I'b t Thr 70 75 aag at c cgt cct gga t ac caa gct gaa gt a at t Lys Ilie Ar g Pr o G31y Ty r (31n Ala (31u Val Ilie 90 gct ct g ct g t cc t tc cga ggt gca at c gt t gac Ala Leu Leu Ser Phe Arg G31y Ala Ilie Val Asp 100 105 ggt cct t tc t gt gt t at c gac ct t ggc ggt gga G31y Pr o Phe Cys Val Ilie Asp Leu G31y 31y G31y 115 120 125 gt t ggc acc t ac gac ggt gaa at c ct a ggc t cc Val G31y Thr Tyr Asp G31y 31u Ilie Leu G31y Ser 135 140 gga tgc gtg cgc ctg acc gaa cga atc atg cgc G31y Cys Val Ar g Leu Thr O31u Arg Ilie Nib t Ar g 150 155 tgaaaccgaa gtggaaatcg ccc c ag O31n (31y cct Pr o act Thr t ca Ser g ac As p ct t Le u g aa (31u g aa O31u gag O31u ac c Thr cca Pr o 1 <210O> 106 <211> 162 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 106 Ou Val Thr Pro Ou Gy Phe Lys Ou 1 5 Val Ar g Leu G31y Lys G31y Val As p Ala Ala I Ie O31u Ar g Thr Ar g Val Ala Leu 40 O31u Thr Hi s G31y Val O31u Ala Val Ar g Ar g As p Ala Ser As n Ar g Asp O31u Phe 70 Leu Ser Lys I Ie Ar g Pr o G31y Ty r O31n O31u O31u Ala Leu Leu Ser Phe Ar g G31y 100 105 Asp O31n G31y Pr o Phe Cy s Val I Ie As p 115 120 Phe I Ie Val G31y Thr Ty r As p (31y O31u 130 135 O31n 'b t G31y Cys Val Ar g Leu Thr (31u 145 150 Pr o As p lIe 10 Thr O31u vb t Ph e A a 90 A a Le u I Ie Ar g Thr G31y As n ValI Ser O31u I Ie G31y Le u I Ie 1 O31u Le u ValI Thr Thr I Ie As p G31y 125 Ser Ar g Thr Pr a Le u A a O31n O31y Pr a Th r Se r As p Page 125 seqi i st car r ect ed. t xt <210O> 107 <21 1> 654 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (631) <223> RXS01835 <400> 107 t caacat ct a t t cct cct gc gat t t gcat g ggat at at at t aaaaat tct agccgaaagt t tcct gcgt g aat acact tt ccccgcgcct t cgcaaagct t gg Tr p ag c Ser g aa O31u ccc Pr o gt g ValI aaa Lys tt a Le u t cc Ser tt t Ph e c ag O31n 1 50 ggg G31y c ac Hi s ct t Le u gt g ValI g aa O31u gag O31u cgt Ar g gct A a tt g Le u 120 gct A a t ct Ser t gc CYS c ac Hi s t ca Ser at c I Ie aat As n gt g ValI g ac As p tt a Le u g ac As p aaa Lys c ag O31n g aa O31u 1 70 cac Hi s gt g ValI gcc A a t ct Ser gaa O31u 75 gct A a at c SI e ct t Le u gag O31u cgc Ar g 155 gt a ValI at g vb t ggg O31y gcc A a gag O31u t ac Ty r t cc Ser g aa O31u c ag O31n 140 tt a Le u cgc Ar g t ac Ty r ct g Le u 45 c aa O31n ct c Le u ct c Le u ac c Th r at a I Ie 125 c aa O31n ggg O31y aaa Lys tt g Le u ct c Le u ct t Le u ac c Th r ct g Le u gca A a 110 c ac Hi s at c I Ie tt c Ph e ggc O31y 15 ct g Le u c ac Hi s g aa O31u aaa Lys c ac Hi s 95 g at As p ggt O31y t gg Tr p aat As n ggt O31y gcc A a g ac As p gct A a c ag O31n ct c Le u aaa Lys g aa O31u t gg Tr p gag O31u 1 at g vb t 1 gga O31y ag c Ser ac c Th r g at As p ccc Pr o ag c Ser ct g Le u g at As p t at Ty r 145 tt g Le u aat act gcc As n Th r A a at t I Ie gt c ValI ct c Le u tt t Ph e tt a Le u gca A a cat Hi s tt a Le u 130 ag c Ser aat As n t at Ty r aat As n g ac As p ccg Pr o ag c Ser gcc A a 1 00 ct c Le u c aa O31n gt t ValI c aa O31n aag ct c t ta aag Lys Leu Leu Lys caa agc gcc O3 n Se r A a 1 t aggcgct cg gcggcgt cga t aa <210> 108 <211> 177 <21 2> PmT Page 126 seqI i st car r ect ed. t xt <21 3> Cor ynebact er i urn gi ut anm curn <400> 108 I'bt Asn Thr Ala Ala Trp Ala His Arg G31y Ser Th r As p Pr a Ser Le u As p Ty r 145 Le u Al a Pr a Th r (31u G31y Lys5 G31y As n 115 Tr p (31u Lys5 ValI As p ValI Ar g Tr p Ser vb t Ar g Ty r Le u 1 65 Hi s As p (31u Ar g Al a (31u I Ie As n 135 li1e Le u Le u ValI 40 (31u (31u Ar g Al a Le u 120 Al a Ser Cys Ty r Le u Ty r Le u Al a ValI 1 As p G31y Le u ValI Hi s ValI Al a Ser (31u Al a I Ie Le u (31u Ar g 1 Lys5 Ar g Ty r Le u O31n Le u Le u Th r I Ie 125 O31n G31y Le u G31y Le u Hi s O31u Hi s As p G31y Tr p As n O31n 1 <210O> 109 <21 1> 1050 <212> DNA <213> Cor ynebact er i urn gI ut anm curn <220> <221 OIDS <222> (1027) <223> RXS02497 <400> 109 t cgat gccgc cgct ggcgaa gact cgggga aacct aaaaa gat tcacact t tgccaccct agaccgt ct a acct t taggt t accgaagaa gaat t t gacc gt g aga t ta ggt gt a t ta gat gt g Leu Asp Val ccc ggt gga Pr o 31y G31y ct t gt t gag Leu Val (3u ggc agc aat act gt c cac G31y Ser Asn Thr Val Hi s cac ccc acc ccg atg agc Hi s Pr o Thr Pro I'b t Ser 30 ct t ct t gat gac t cc ggg Leu Leu Asp Asp Ser (31 y 45 Val Ar g Leu (31y Val 1 ct a gt t gca gt a gac gcg cgt Leu Val Al a Val As p Al a Ar g 15 aat t gg cgt acc cca ct g cgc As n Tr p Ar g Thr Pr o Leu Ar g gcg at c t cc gaa aag ggc at c Al a Il e Ser (31 u Lys (31 y Il e Page 127 seql i st cor r ect ed. t xt aac aaa Asn Lys ct c ggc Leu G y gcc acc Al a Thr gtc cgc Val Arg ctc gca Leu Al a ctc gac Leu Asp 135 t cc cca Ser Pro 150 cac aac His Asn ctg cgc Leu Arg cgc acc Arg Thr cgc acc Arg Thr 215 cac gt c His Val 230 ttt atc Phe Il e agc tcg Ser Ser gct gcg Al a Al a gca ctt Al a Leu 295 ctc acc Leu Thr tgc gct Cys Ala aac agc Asn Ser ctg t cc Leu Ser 105 gtt cga Val Arg 120 at c ggt lie G y gac ct c Asp Leu tgg ttc Trp Phe gat tat Asp Tyr 185 cta ggg Leu G y 200 ctg gca Leu Al a acc cga Thr Arg t ca cga Ser Arg gat cgg Asp Arg 265 atg cgt lIet Arg 280 cgt gaa Arg G u t ca Ser gaa G u gag Su at c SIle cgt Ar g ggc G y gcg Al a gac Asp 170 at c SIle ccc Pr o cga Ar g acc Thr at g Met 250 t ca Ser gcg Al a ggt Sy gca Al a ct g Leu 75 gca Al a ctt Leu tgg Tr p ggc G y tt c Phe 155 acc Thr gat Asp gcg Al a ct g Leu ct c Leu 235 act Thr cat Hi s tt g Leu gt g Val gt c Val 60 at g Met gt g Val t cc Ser t at Tyr t cc Ser 140 t ca Ser gat Asp gcg Al a cgc Ar g act Thr 220 acc Thr gcg Al a cag G n gat Asp at c SIle 300 ggg gaa gca gca G y G u Al a Al a cca Pr o ct c Leu ggt G y gga G y 125 ct g Leu ct g Leu cca Pr o gaa Gu ct g Leu 205 ggt Gy gcg Al a gcg Al a at c SIle at t SIle 285 ct c Leu ttt Phe gac Asp gaa Gu 110 tgg Tr p gaa Gu gat Asp ccg Pr o ctt Leu 190 gca Al a gct Al a ccg Pr o gac Asp gt g Val 270 gac Asp acc Thr gct Al a cac Hi s 95 gac Asp t cc Ser ct a Leu ct g Leu gca Al a 175 gca Al a gt g Val gcg Al a ggt G y cgc Ar g 255 gca Al a aag Lys agg Ar g aca Thr 80 gt g Val gaa Gu gca Al a t cc Ser ggt Gy 160 cgt Ar g gaa Gu gga Gy ccc Pr o ct g Leu 240 gct Al a ggt G y gt a Val at c SIle gac Asp tcg Ser gag G u gca Al a ggg G y t cc Ser 145 gcg Al a aag Lys ccc Pr o aca Thr t ca Ser 225 cgc Ar g gag G u gcg Al a gaa Gu gac Asp 305 cta gcg Leu Al a gca gt c Al a Val aag gaa Lys G u cgc caa Arg G n 115 cgc at a Arg Ile 130 gga acc G y Thr ggc cgc G y Arg aaa at c Lys II e gcc cgc Al a Ar g 195 tcc aaa Ser Lys 210 t cc gca Ser Al a cag ctg G n Leu ct g aa Leu G u ct a gt t Leu Val 275 att tgt I e Cys 290 aaa gga Lys G y aaa Lys cgc Ar g acc Thr 100 act Thr act Thr gac Asp ttg Leu aac Asn 180 cag G n act Thr gga Gy at c SIle ggt G y 260 gcg Al a ccg Pr o ct c Leu acg Thr t cc Ser G y ttc Phe aac Asn gaa G u acc Thr 165 ct c Leu at g let ttc Phe cca Pr o gca Al a 245 at c SIe gaa G u tgg Tr p gag G u 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1050 taacatttac ccggaaagga gtt Page 128 seql i st cor r ect ed. t xt <210> 110 <211> 309 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 110 Val Arg Leu y Val 1 5 Ala Val Asp Ala Arg Arg Thr Pro Leu Arg Ser G u Lys G y II e Asp Leu Ala Lys Thr Ser Ala Val Arg Ser G u Lys G u Thr G y 100 Ala Arg G n Thr Phe 115 Sy Arg lie Thr Asn 130 Ser G y Thr Asp G u 145 Ala y Arg Leu Thr 165 Lys Lys Ile Asn Leu 180 Pro Ala Arg G n IVet 195 Thr Ser Lys Thr Phe 210 Ser Ser Ala y Pro 225 Arg n Leu lie Ala 245 G u Leu G u y Ile 260 Ala Leu Val Ala G u 275 Su Ile Cys Pro Trp 290 Asp Lys G y Leu G u 305 <210> 111 Leu Asp Val G y Pr o Leu Asn Leu 70 Al a Val Leu Leu Ser 150 Hi s Leu Ar g Ar g Hi s 230 Phe Ser Al a Al a Sy Val Lys Sy Thr Ar g Al a Asp 135 Pr o Asn Ar g Thr Thr 215 Val SIle Ser Al a Leu 295 Hi s 25 Leu Thr Al a Ser Ser 105 Ar g Sy Leu Phe Tyr 185 Sy Al a Ar g Ar g Ar g 265 Ar g G u Ser 10 Pr o Leu Ser G u G u 90 I e Ar g Sy Al a Asp 170 I le Pr o Ar g Thr bet 250 Ser Al a Sy Asn Thr Asp Al a Leu Al a Leu Tr p Sy Phe 155 Thr Asp Al a Leu Leu 235 Thr Hi s Leu Val Val Met Ser Sy Pr o Leu Sy Sy 125 Leu Leu Pr o G u Leu 205 Sy Al a Al a SIle SIle 285 Leu Hi s Ser Sy G u Phe Asp G u 110 Tr p G u Asp Pr o Leu 190 Al a Al a Pr o Asp Val 270 Asp Thr Leu Asn Al a Al a Al a Hi s Asp Ser Leu Leu Al a 175 Al a Val Al a Sy Ar g 255 Al a Lys Ar g Page 129 NO 2seql i st cor r ect ed. t xt <211> 534 <212> DNA C- <213> Cor ynebact eri um gl ut ai cum <220> d) <221> CDS L <222> (101)..(511) S<223> RXS02972 c <400> 111 acctacgacg gtgaaatcct aggctcccac tcaacccaaa tgggatgcgt gcgcctgacc gaacgaatca tgcgcagcga cccacccgac tgaaaccgaa gtg gaa atc gcc cgc 115 0 Val u Ile Ala Arg 00 1 0 gac tac gtt gca gaa cgc atc cag gaa gta aaa gcc atc gtc cca att 163 C- Asp Tyr Val Ala u Arg Ile n u Val Lys Ala lie Val Pro Ile 10 15 Stca aag gca aaa acc ttt gtg gga tgc gca ggc acc ttc acc aca atc 211 C- Ser Lys Ala Lys Thr Phe Val y Cys Ala y Thr Phe Thr Thr lie 30 tcc gcc tgg gtg caa ggc cta gaa agc tac gac cgc gac gcg atc cac 259 Ser Al a Trp Val G n G y Leu G u Ser Tyr Asp Arg Asp Al a lle His 45 ctc tct gca ctc aac tt c gat gca ctg cga gtt gtc acc gat gag atc 307 Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val Val Thr Asp G u Ile 60 att tca gaa tca tca tca cag cgc gcc agc aac cca gtt gtt gat cca 355 Ile Ser u Ser Ser Ser G n Arg Ala Ser Asn Pro Val Val Asp Pro 75 80 ggt cgc gcc gac gtc atc ggt ggc gga tcc gtt gtt gtc caa gca gcg 403 Sy Arg Ala Asp Val Ile G y G y y Ser Val Val Val n Ala Al a 95 100 atc gac tta gcc tcc aaa gaa gcc ggt gta gac tac atc att att tcc 451 Ile Asp Leu Ala Ser Lys u Ala y Val Asp Tyr lie lie lle Ser 105 110 115 gaa aaa gac atc ctc gac ggc ctc atc ctt ggc ctg gta gaa gcc gac 499 G u Lys Asp Ile Leu Asp y Leu Ile Leu y Leu Val G u Ala Asp 120 125 130 tct ttg aag aaa taggacccta gttttaaacc act 534 Ser Leu Lys Lys 135 <210> 112 <211> 137 <212> PRT <213> Cor ynebact er i um gl ut ai cum <400> 112 Val u Ile Ala Arg Asp Tyr Val Ala u Arg Ile On u Val Lys 1 5 10 Ala I e Val Pro Ile Ser Lys Ala Lys Thr Phe Val y Cys Ala y 25 Thr Phe Thr Thr lie Ser Ala Trp Val n y Leu u Ser Tyr Asp 40 Page 130 seql i st cor r ec Arg Asp Ala lie His Leu Ser Ala Leu Asn Phe 55 Val Thr Asp u Ile Ile Ser u Ser Ser Ser 70 75 Pro Val Val Asp Pro G y Arg Al a Asp Val lie 90 Val Val G n Ala Ala I e Asp Leu Ala Ser Lys 100 105 Tyr Ile Ile Ile Ser u Lys Asp Ile Leu Asp 115 120 Leu Val G u Ala Asp Ser Leu Lys Lys 130 135 <210> 113 <211> 636 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(613) <223> RXA02159 <400> 113 tgatggacca gcgtccaaag ttttcgatga agcagaaaac actgctggtg tggctgctgg ccaaccagcc gaggtaagac ted. txt Asp Ala Leu Arg Val G n Arg Ala Ser Asn G y G y G y Ser Val Su Ala y Val Asp 110 G y Leu I e Leu G y 125 cgcctccacg ctcagaaagc atg tcc ctt ggc tca 11! IVet Ser Leu G y Ser acc Thr caa G n gt a Val gcc Al a ccc Pr o gcc Al a gat Asp cgc Ar g t ca Ser ct c Leu ct g Leu ttg Leu ggg Gy gaa G u ct g Leu ccg Pr o 120 ccg Pr o ttg Leu gaa G u cga Ar g cgc Ar g ct c Leu gtt Val gga Gy gaa G u cag G n tt g Leu gat Asp gcc Al a 75 cgg Ar g tct Ser gct Al a aac Asn at t SIle ct g Leu ct c Leu t ac Tyr ggt G y aca Thr gcc Al a tta Leu ttg Leu ct g Leu 45 gat Asp t ac Tyr ccg Pr o gat Asp cag G n 125 aat Asn gac Asp gat Asp gaa G u gcg Al a tcg Ser cat Hi s 110 t ac Tyr ccc Pr o 15 aaa Lys gaa G u ct c Leu gt c Val gag G u 95 t cc Ser ct g Leu gt g Val caa G n ggc Sy ggt G y ggc G y aag Lys ggc G y gca Al a 1 act Thr aaa Lys at c SIle gca Al a cca Pr o ct g Leu aac Asn agt Ser cgc Ar g gt c Val gat Asp cgc Ar g gt a Val cgc Ar g at c SIle ttc Phe 130 act Thr acc Thr at c SIle aag Lys gat Asp cgc Ar g gcg Al a 115 at c SIle gca Al a agc Ser acc Thr gtt Val agc Ser at g Met 100 at g Met gat Asp Page 131 gt g ggg ct g Val G31y Leu 135 t tc gt t ct c Phe Val Leu 1 50 ct C agc ggg Leu Ser G3 y g aa (31u cgt Ar g ac C Th r 1 seqi i st car r ect ed. t xt gt t ggc acc atc gct ggt gat gac acc gt t Val G31y Th r I Ie Ala G31y As p As p Th r Val 140 145 ccg ct c aca ggt aaa gaa ct a ggt gaa tt a Pr o Leu Thr G31y Lys G31u Leu G31y G31u Leu 160 165 t aaagcgccc ct agt tcaag gct <210> 114 <211> 171 <21 2> PRT <21 3> Cor ynebact er i urn gl ut ani curn <400> 114 vb t Th r Ly s I Ie A a Pr o Le u As n Ser G31y 145 (31u Ser Leu G31y Ar g Thr Ala Val Thr Ser As p I I e Thr Ar g Lys Val Val As p Ser Ar g Ar g Nib t 1 00 I e A a Nib t 115 Phe I I e As p 130 As p As p Thr Leu G3 y G3 u Ser Ar g G n O3n Ar g I Ie Le u Le u Ar g ValI Le u 1 Thr Pro (3 n A a Val G31n A a Thr 55 Pr o As p 70 A a Ar g As p (1u Ar g Thr Val G31y 135 Phe Val 150 Leu Ser Th r I Ie Ser Ser G31y As p Le u 1 Pr o Ly s A a Ar g O31u O31n Le u As p A a Ar g Ser A a ValI As p 1 Th r As n I Ie Le u Le u Ty r G31y Th r A a ValI 140 Pr o Le u Le u Le u As p Ty r Pr o As p O31n 125 G31y Le u Pr a Ly s G u Le u ValI (3u Ser Le u I Ie (3y <210O> 115 <21 1> 486 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani curn <220> <221 CDS <222> (463) <223> RXA02201 <400> 115 tct accagcc aaat cat caa ct cat agcga aggaat caac t t cat gaat a at caaccat c agt actt t tc gt t tgcgt cg gcaat ggt gg aaaat ct caa at g gcc gca gcg ct a 115 Nib t A a A a A a Le u Page 132 gcc Al a aag Lys gtt Val tta Leu ct a Leu gac Asp gt c Val acc Thr aaa Lys ggt Sy gca Al a aag Lys at g Met ccc Pr o gat Asp aac Asn 120 seql i st cor r ect ed. t xt 1 gcc ggg gac gct ctc aaa gtt tat tca Ala G y Asp Ala Leu Lys Val Tyr Ser 15 aaa tta aat caa cag tcc ctt gat tcc Lys Leu Asn G n G n Ser Leu Asp Ser atg tct caa ggg ttt cca aag ggc att IVet Ser n y Phe Pro Lys y lie 45 gta gac cgc gtg gtc att ctt ggt gcc Val Asp Arg Val Val lie Leu y Al a atc gat gca aac ggc ata cta cag cgc lie Asp Ala Asn G y lie Leu n Arg 75 80 gaa cgt gga att gaa ggt atg gaa cgc G u Arg y lie G u y IVet u Arg 95 att gac gcc cga gtc caa aac ctc gtc Ile Asp Ala Arg Val G n Asn Leu Val 110 tagcagtttt ctaatctcac aca gct Al a at t SIle gac Asp gaa G u tgg Tr p at g Met gct Al a 115 ggc gct Al a cag G n gct Al a gt a Val cgc Ar g 100 gaa G u aca Thr gaa G u gag G u caa G n act Thr ctg Leu ct a Leu <210> 116 <211> 121 <212> PRT <213> Corynebacter i um gl utam cum <400> 116 IVet Ala Ala Ala Leu Ala Lys Lys His 1 5 Tyr Ser Ala y Thr Lys Pro y Thr Asp Ser I e Ala u Val y Ala Asp 40 Sy Ile Asp n u Leu Ile Lys Arg 55 Sy Ala u Ala n Leu u bet Pro 70 G n Arg Trp Val Thr Asp G u Pro Ser G u Arg IVet Arg Leu Val Arg Asp Asp 100 105 Leu Val Ala G u Leu Thr G n Asn Ala 115 120 <210> 117 <211> 510 Al a 10 Lys bet Val SI e G u 90 I e Sy Leu Ser Asp Asp Ar g Asp Asp Asn G n Ar g Al a G y Al a Al a G n Sy Val Asn SIle Ar g Lys Ser Pr o SIe SIe Sy G n Page 133 seal i st cor r ect ed. t xt <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(487) <223> RXA00599 <400> 117 gaacgatcgg ccctttgatt gaagtcccag tattagtcgg ggcttggacc aaaaatcttt aaaaaggaga atgcaggatc attggtttat gtcatgttgt ttt gtg tgc Phe Val Cys gca cag aag Al a G n Lys aag cct gca Lys Pr o Al a gt g ggc gct Val G y Al a ctg ctg cgc Leu Leu Arg gta gat at g Val Asp Vbet gag gaa ccg G u G u Pr o cag at c gat G n Ile Asp 120 ggt Gy gca Al a ggg Gy at g Met gt c Val gaa G u gct Al a aat Asn t ca Ser ct a Leu tcg Ser gat Asp 75 tct Ser caa G n ggc Gy gat Asp aac Asn caa G n 60 cgt Ar g gca Al a ggt G y gga Gy t ca Ser caa G n 45 gga G y gt g Val cag G n at g Met gct Al a 125 aaa Lys gt a Val ttg Leu at t SIle gtt Val ggc G y gaa G u 110 t ca Ser 15 gag G u tct Ser ccc Pr o at t SIle gct Al a 95 cgt Ar g cag G n at c SIle gt g Val aaa Lys ttg Leu 80 ctt Leu at g Met at g Met 1 at g Met cat Hi s gaa G u gcg Al a ggc G y gag G u cgt Ar g aaa tca gtt ttg Lys Ser Val Leu gcg Al a tct Ser t cc Ser at c l Ie gat Asp cgt Ar g at t SIle gcg Al a gct Al a at c SIle gat Asp gac Asp tgg Tr p gt g Val gcg ctg Al a Leu gga acc G y Thr gct gag Al a u ccg gag Pro G u gca cag Al a n tca att Ser Ile 100 cgg gat Arg Asp cga gt c caa Arg Val n ttg cta gcg gga Leu Leu Ala G y taagcgccga aaaaggggca tgt <210> 118 <211> 129 <212> PRT <213> Corynebacteri um gl utami cum <400> 118 et Lys Ser Val Leu Phe Val Cys Val y Asn y y Lys Ser G n 1 5 10 IVet Ala Ala Ala Leu Ala G n Lys Tyr Ala Ser Asp Ser Val G u Ile 25 His Ser Ala G y Thr Lys Pro Ala G n y Leu Asn G n Leu Ser Val 40 Su Ser I e Al a u Val y Ala Asp Iet Ser G n y Ile Pro Lys 55 Page 134 seqI i st car r ect ed. t xt Ala lieAsp Pro Gu Leu Leu Arg Thr Val Asp Arg Val Val I Ie Leu 70 75 31y Asp Asp Al a 1n ValI As p lb t Pro (1u Se r Aia (1n 1y Ala Leu 90 G31u Arg Trp Ser I Ie 31u G31u Pro As p Al a 31n G31y 'b t O31u Ar g I'b t 100 105 110 Ar g I Ie Val Ar gAs p 1n IlIe Asp As nArg Val 31n Al aLeu Leu Ala 115 120 125 G31y <210O> 119 <21 1> 1221 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1198) <223> RXA00600 <400> 119 cggagt taat gagcggt agg t ggat gggt g cggt cat gt c cgt cat tat a t at tgacgca cat cgat at t gaaggt at tt t tat atcggc aaacat caat ct c Le u at g vb t gct Al a tt g Le u tt g Le u act Thr tt g Le u tt t Ph e ct t Le u ac c Thr tt t Ph e ggg G31y gcg Al a gt c ValI g aa (31u t gg Tr p cca Pr o 120 cgc Ar g act Thr g ac As p tt a Le u g aa (31u at g vb t ag c Ser gt t ValI c aa O31n at c I Ie aaa Ly s cgc Ar g at c I Ie at t I Ie t at Tyr 75 aca Thr gga G31y cca Pr o gcg Al a gag G31u t gg Tr p ggc G31y ggt G31y cca Pr o g ac As p cca Pr o gag G31u at g 140 cat Hi s tt a Le u aag Ly s 45 gga G31y cct Pr a cgc Ar g gca Al a ct t Le u 125 gt t ValI t cg Ser gct Al a gt c ValI at t I Ie tt g Le u gct Al a ct t Le u 110 cgc Ar g tt g Le u ac a Thr 15 gcc Al a tt t Ph e t cc Ser gcc Al a ct c Le u 95 at g vb t act Thr cct Pr a t gg Tr p cca Pr a at t I Ie aag Ly s 80 at g vb t tt t Ph e ggg (31y at g vb t 1 cga Ar g at t I Ie gga G31y cca Pr a gt g ValI gt g ValI ag c Ser ct a Le u at t gaa ggc t gg le 31u G31y Tr p gcg gct ggc t ca Al a Al a 03 y Ser t tc t tg gct at g Phe Leu Al a I'b t at t ggg gcg ct t Il e 03 y Al a Leu at t gct at c ggt Ile Al a IlIe 03 y cgc t ac gac aaa Arg Tyr Asp Lys gt g t cg at t at g Val Ser Ilie Nib t 1 00 ct g gcg t gg ct g Leu Ala Trp Leu 115 at t at c gt g ggc Ilie Ilie Val G31y 130 gta tgg agt gat ctc gct tgt Val Tr p Ser Asp Leu Al a Cys 145 Page 135 seql i st cor r ect ed. t xt ggt Gy 150 cag G n ccc Pr o t ca Ser gga G y t ac Tyr 230 ttg Leu aca Thr t ac Tyr ggg Gy aac Asn 310 aca Thr cca Pr o at c SIle tgc cgg Ar g ctt Leu tgg Tr p gt g Val 200 tta Leu gac Asp t ac Tyr cag G n gt g Val 280 act Thr ttt Phe ccg Pr o tta Leu aaa Lys 360 gca Al a ttc Phe 170 gga G y t cc Ser cgc Ar g ttc Phe at t SIle 250 tgg Tr p at g Met gag G u tta Leu gca Al a 330 gga G y act gct gtg ctg gtt gca Thr Ala Val Leu Val Ala 155 160 ggt gtg ctt ggt tgg ttt G y Val Leu Gy Trp Phe 175 tta gac acc acg tcg gtg Leu Asp Thr Thr Ser Val 190 gtt ctc gtg ttc ttg ggc Val Leu Val Phe Leu G y 205 gtc att ggt gaa aaa aca Val I e Gy u Lys Thr 220 ctg cct aag att tca ccc Leu Pro Lys Ile Ser Pro 235 240 gtt ctg ctg ttt tcg ttg Val Leu Leu Phe Ser Leu 255 aca gta gct cgt ctt gca Thr Val Ala Arg Leu Ala 270 ttt ttc att tcc ctg gtg Phe Phe lie Ser Leu Val 285 cga gct gct tcc gtg tct Arg Ala Ala Ser Val Ser 300 gcg att gcg gta tcg atc Ala lie Ala Val Ser Ile 315 320 tta gct gga acg atc ggc Leu Ala Gy Thr Ile y 335 ttg gtt tat gtc atg ttg Leu Val Tyr Val IVet Leu 350 at c aac t cg li e Asn Ser t ac ct g cag Tyr Leu G n act ttc tct Thr Phe Ser 195 at a cca ct t Ile Pro Leu 210 aag gga cgg Lys G y Ar g 225 ttg gcg ctg Leu Ala Leu cag ggg gat Sn y Asp ttg ccg ctg Leu Pro Leu 275 gt a t cc aaa Val Ser Lys 290 ttt act gca Phe Thr Al a 305 gga acc ttt G y Thr Phe cct ttg att Pro Leu Ile tgg ctt gga Trp Leu G y gt g Val at t SIle 180 gt g Val gt a Val cgc Ar g at t SIle gaa G u 260 ct g Leu ct g Leu gca Al a ggt Gy gaa G u 340 cca Pr o ttc Phe 165 ctt Leu gt a Val gct Al a tgg Tr p ggc G y 245 at c SIe at g Met t cc Ser gga Gy gcg Al a 325 gt c Val aaa Lys 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1218 1221 aag gag aat gca Lys G u Asn Al a gga t ca G y Ser 365 tgaaatcagt tttgtttgtg <210> 120 <211> 366 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 120 et I I e G u G y Trp Leu Iet Thr Leu Thr Lys G u His Ser Thr Pro 1 5 10 Page 136 seql i st cor r ect ed. t xt Ar g SIle Gy Pr o Val Val Ser Leu Ser 145 I le Tyr Thr SIle Lys 225 Leu G n Leu Val Phe 305 Gy Pr o Tr p Al a Al a G y Phe Leu Al a i e G y Al a I e Al a I le Arg Tyr Asp Val Ser Ile 100 Leu Ala Trp 115 Ile Ile Val 130 Asp Leu Al a Asn Ser Val Leu G n Ile 180 Phe Ser Val 195 Pro Leu Val 210 G y Arg Ar g Al a Leu Il e Sy Asp u 260 Pro Leu Leu 275 Ser Lys Leu 290 Thr Al a Al a Thr Phe G y Leu Ile G u 340 Leu G y Pro 355 Ser IVet Ser Phe Leu Asp Arg Trp Leu Ala Ala Trp 25 bet Leu Gy Lys vbt Leu Gy Cys Phe 165 Leu Val Al a Tr p Gy 245 I le vbt Ser Gy Al a 325 Val Lys Al a Leu Leu 70 Thr Leu Phe Leu Gy 150 G n Pr o Ser Gy Tyr 230 Leu Thr Tyr Gy Asn 310 Thr Pr o SIle Al a Ser 55 I le Lys Asn Leu Al a 135 Asp SIle Ser SIle Val 215 G u Leu Al a Phe Leu 295 Asn Ser Val Phe Gy 40 Al a Val G u Tr p Pr o 120 Ar g Ar g Leu Tr p Val 200 Leu Asp Tyr G n Val 280 Thr Phe Pr o Leu Lys 360 Leu Val Mbet SIle SIle 105 Asp Cys G u bet Leu 185 Thr Ser Thr Thr Pr o 265 Gy Tyr G u G n Val 345 Lys Leu G u bet Ser 90 Val G n SIle Al a Phe 170 Sy Ser Ar g Phe SIle 250 Tr p Met G u Leu Al a 330 Sy G u I e I e Tyr 75 Thr Sy Pr o Al a Thr 155 Sy Leu Val Val Leu 235 Val Thr Phe Ar g Al a 315 Leu Leu Asn Sy Sy Pr o Asp Pr o G u Lb t 140 Al a Val Asp Leu SIle 220 Pr o Leu Val Phe Al a 300 l le Al a Val Al a Lys Sy Pr o Ar g Al a Leu 125 Val Val Leu Thr Val 205 Sy Lys Leu Al a SIle 285 Al a Al a Sy Tyr Sy 365 Val SIle Leu Al a Leu 110 Ar g Leu Leu Sy Thr 190 Phe Su I e Phe Ar g 270 Ser Ser Val Thr Val 350 Ser Phe Ser Al a Leu vbt Thr Val Val Tr p 175 Ser Leu Lys Ser Ser 255 Leu Leu Val Ser SIle 335 vbt Pr o SIe Lys vbt Phe Sy Tr p Al a 160 Phe Val Sy Thr Pr o 240 Leu Al a Val Ser SIe 320 Sy Leu Page 137 seql i st cor r ect ed. t xt <210> 121 <211> 1233 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1210) <223> RXA02200 <400> 121 at t gtgtgga gagtggtcat aaatccacta tatat t gacg tttgaatatc gacaggtatc aatataccga aaggtgtcgc aat gt cgat a t t gaaagt at cag G n cca Pr o agc Ser at c SIle ttg Leu cat Hi s tta Leu cga Ar g ttg Leu ct c Leu 150 ggc G y acc Thr ttc Phe cgg Ar g tgg Tr p tcg Ser ttg Leu aaa Lys at g Met ttc Phe ggc G y 120 tgg Tr p gcc Al a ttc Phe gct Al a gga G y 200 aag Lys at t SIle ct c Leu at c SIle cgg Ar g gt g Val ct a Leu at t SIle gat Asp aat Asn ct g Leu 170 ttc Phe cct Pr o cca Pr o tt g Leu t ca Ser gct Al a t at Ty r 75 t ca Ser gct Al a at t SIle at g bet t ca Ser 155 caa G n tct Ser ct a Leu gcc Al a gcg Al a ggc Gy tta Leu gac Asp ct c Leu tgg Tr p gt a Val t cc Ser 140 gtt Val gtt Val ttc Phe ctt Leu cga Ar g at g Met tt t ttt Phe 45 ggc Gy aaa Lys at t SIle ttg Leu gga G y 125 tgt Cys ttt Phe tta Leu tgg Tr p gct Al a 205 at c SIle gcg Al a ct a Leu ct c Leu act Thr ct c Leu ttc Phe 110 ct c Leu gga G y caa G n cca Pr o t ca Ser 190 gga Gy t ca Ser 15 ttt Phe ggc Gy ctt Leu aaa Lys aat Asn 95 ct c Leu gca Al a gac Asp gt c Val t cc Ser 175 at t SIle gtt Val ttt Phe ggg G y gca Al a gt a Val caa G n 80 tgg Tr p cca Pr o cga Ar g cgc Ar g gca Al a 160 tgg Tr p gt g Val ttc Phe at g Met 1 ctt Leu ct a Leu at g let at g Met at t SIle gt g Val gac Asp tgt Cys gag G u 145 at g Met ct a Leu act Thr tcg Ser gat Asp ttt Phe gaa G u at g Met gcc Al a gt g Val caa G n at t SIle 130 gct Al a ttt Phe gga G y tcg Ser cga Ar g 210 aaa Lys tta Leu gt c Val t ac Tyr act Thr ggt G y ccg Pr o 115 gcg Al a aca Thr ggt G y tta Leu gtt Val 195 at t SIle t ac Tyr ggc G y gga G y cca Pr o gat Asp cct Pr o 100 gaa G u at g Met gca Al a gca Al a cca Pr o 180 ttg Leu at t SI e aca aac tca Thr Asn Ser act Thr att SIe cgg Ar g ggg G y ccg Pr o aag Lys gcc Al a tta Leu gtc Val gtt Val ctt Leu 165 act Thr gtg Val ggc Gy Page 138 seql i st cor r ect ed. t xt gaa aag atc aag G u Lys Ile Lys 215 atc tct cca ttt Ile Ser Pro Phe 230 ttt tca ttg caa Phe Ser Leu G n cgt ctc gcg ata Arg Leu Ala lle 265 tca ctc att gcg Ser Leu Ile Ala 280 tcc gtc tct ttc Ser Val Ser Phe 295 gtg tcg atc gga Val Ser I e G y 310 acg att ggt ccc Thr lie Gy Pro gcc atg ctg tgg Ala IVet Leu Trp 345 cca t ca t ca gct Pro Ser Ser Ala 360 at caact tca t ga <210> 122 <211> 370 <212> PRT <213> Corynebact <400> 122 IVet Thr Asn Ser 1 Leu Asp Lys Tyr Leu Phe Leu G y V t Gu Val y IVet IVet Tyr Pro Ile Al a Thr Asp gga G y gca Al a ggt Sy 250 cca Pr o t ca Ser act Thr acg Thr ttg Leu 330 ct a Leu cgt Ar g ct a Leu 235 gat Asp tt g Leu aaa Lys gca Al a ttt Phe 315 at t SIle ggc G y gag tgg Su Trp 220 at c ggt lie G y cag at c Gn lle gtt atc Val lile ct a t ct Leu Ser 285 gct ggc Al a G y 300 ggc gca G y Al a gaa att G u lie ccc aag Pro Lys tat gaa Tyr G u ctg ctt Leu Leu gt c t ct Val Ser 255 tat ttc Tyr Phe 270 ggc at g G y bt aac aat Asn Asn act tct Thr Ser cca gt a Pro Val 335 ttg ttc Leu Phe 350 caa aag ttc ctt G n Lys 225 tat acg Tyr Thr 240 caa cca G n Pro gtt gga Val G y aac t at Asn Tyr ttt gaa Phe G u 305 gca cag Al a G n 320 ctt gtc Leu Val cca aat Pro Asn Phe Leu at c gt c Ile Val tgg gct Trp Al a atg ttt bet Phe 275 gca aag Al a Lys 290 ctt gcg Leu Al a gct atg Al a Vbet ggc ttg G y Leu gac ccc Asp Pro 355 ccg Pr o ttg Leu gt a Val 260 ttc Phe tct Ser at t l Ie gca Al a gt c Val 340 acg Thr gca Al a ttg Leu 245 gtt Val at t SIe gca Al a gcg Al a gga Gy 325 t ac Tyr ct g Leu 787 835 883 931 979 1027 1075 1123 1171 1220 1233 cgt tct acc agc caa atc atc aac tca Arg Ser Thr Ser n lie lie Asn Ser 365 370 tagcgaagga er i um gl ut ai cum Thr G n Thr Arg Ala 5 Ile Pro Leu Trp Ile 25 Arg Ser Val Ser y 40 Gy I e Ser Leu Pro 55 Pro Leu Ala Lys Val 70 Lys His Leu IVet G y Lys Pro Al a 10 I e Leu Al a Leu Ser G y I le Al a Leu Arg Tyr Asp Val Ser Leu 90 Page 139 Ar g Met Phe Gy Lys SIle SIle Al a Leu Leu Thr Leu Ser Phe Gy Leu Lys Asn IN seql i st cor r ect ed. t xt Val Val y Pro Ala Leu Met Phe Ala Leu Ala Trp Leu Phe Leu Pro 100 105 110 Asp n Pro u Leu Arg Thr y Leu Ile I e Val y Leu Ala Arg 115 120 125 4 Cys Ile Ala IVet Val Leu Val Trp Ser Asp Iet Ser Cys G y Asp Arg 130 135 140 G u Ala Thr Ala Val Leu Val Ala lie Asn Ser Val Phe G n Val Ala 145 150 155 160 IVet Phe G y Al a Leu G y Trp Phe Tyr Leu G n Val Leu Pro Ser Trp 165 170 175 00 0 Leu y Leu Pro Thr Thr Thr Ala G n Phe Ser Phe Trp Ser lie Val 180 185 190 \O Thr Ser Val Leu Val Phe Leu y lie Pro Leu Leu Ala y Val Phe 195 200 205 C Ser Arg Ile Ile Gy u Lys Ile Lys y Arg u Trp Tyr u n 210 215 220 Lys Phe Leu Pro Ala lI e Ser Pro Phe Ala Leu Ile y Leu Leu Tyr 225 230 235 240 Thr lie Val Leu Leu Phe Ser Leu G n y Asp n Ile Val Ser G n 245 250 255 Pro Trp Ala Val Val Arg Leu Ala lie Pro Leu Val lie Tyr Phe Val 260 265 270 G y IVet Phe Phe Ile Ser Leu Ile Al a Ser Lys Leu Ser G y IVet Asn 275 280 285 Tyr Ala Lys Ser Ala Ser Val Ser Phe Thr Ala Ala y Asn Asn Phe 290 295 300 G u Leu Ala Ile Ala Val Ser Ile y Thr Phe y Ala Thr Ser Ala 305 310 315 320 G n Ala IVet Ala y Thr Ile y Pro Leu Ile u lie Pro Val Leu 325 330 335 Val y Leu Val Tyr Ala IVet Leu Trp Leu y Pro Lys Leu Phe Pro 340 345 350 Asn Asp Pro Thr Leu Pro Ser Ser Ala Arg Ser Thr Ser n lie lie 355 360 365 Asn Ser 370 <210> 123 <211> 762 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(739) <223> RXA02202 <400> 123 cgctgaacta acccaaaacg catagcagtt ttctaatctc acacatcttc aacaccgtta Page 140 IN seql i st cor r ect ed. t xt aatctattgg tttccccgta aaatcttcga aaggaagaac atg acc ggg caa gct 115 Met Thr G y n Al a 1 gca cca aac ttg cat acc aat att ttg aac cgt atc gca aat gaa ctg 163 Ala Pro Asn Leu His Thr Asn Ile Leu Asn Arg Ile Ala Asn G u Leu 15 C gcg ttg acc tat caa gga gtt ttc tct gca gag act atc aac cgc tat 211 Ala Leu Thr Tyr n G y Val Phe Ser Ala G u Thr Ile Asn Arg Tyr 30 att ttt gaa tcg tat gtg tcg ttg gcg aga aca gca aaa atc cat acg 259 0 lie Phe u Ser Tyr Val Ser Leu Ala Arg Thr Ala Lys lie His Thr 00 40 45 0 0 cac ctg cca att ttg gca gaa ggt ttt gct aaa gac cgg ctg cac gca 307 C His Leu Pro lie Leu Al a G u G y Phe Ala Lys Asp Arg Leu His Ala 55 60 0 ctt gcg gta gct gaa ggt aag gtg gct tca cct gtg cct cag gtc cta 355 Cq Leu Ala Val Ala G u G y Lys Val Ala Ser Pro Val Pro G n Val Leu 75 80 ttt att tgc gtc cac aac gca ggt cgt tca caa att gct tcg gcg ttg 403 Phe Ile Cys Val His Asn Al a G y Arg Ser G n IIe Al a Ser Al a Leu 95 100 ttg tct cac tat gcc ggt agt tct gta gag gta cgt tct gca ggt tct 451 Leu Ser His Tyr Ala G y Ser Ser Val G u Val Arg Ser Ala G y Ser 105 110 115 tta cct gct tct gaa att cac cca ctg gtg ttg gaa att tt g tca gag 499 Leu Pro Ala Ser G u Ile His Pro Leu Val Leu G u Ile Leu Ser G u 120 125 130 cga gga gtg aac att tct gat gca ttt ccg aaa ccg cta acc gat gat 547 Arg y Val Asn Ile Ser Asp Ala Phe Pro Lys Pro Leu Thr Asp Asp 135 140 145 gtt att cgc gca tct gac tat gtc ata aca atg gga tgt gga gat gtg 595 Val Ile Arg Ala Ser Asp Tyr Val I e Thr Iet G y Cys G y Asp Val 150 155 160 165 tgc cca at g tat cca gga aag cac tat ctc gat tgg gag ctc gct gat 643 Cys Pro IVet Tyr Pro G y Lys His Tyr Leu Asp Trp G u Leu Ala Asp 170 175 180 ccg tca gat gaa ggt gag gac aag ata cag gaa ata att gag gaa att 691 Pro Ser Asp G u G y G u Asp Lys lie On G u lie lie G u G u lie 185 190 195 gac ggt cga atc cgc gag ctt tgg aaa agc att caa tta tcg caa aac 739 Asp y Arg lie Arg u Leu Trp Lys Ser Ile n Leu Ser n Asn 200 205 210 t aggcagt ca aaggt ct ggc acc 762 <210> 124 <211> 213 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 124 IVet Thr G y G n Al a Ala Pro Asn Leu His Thr Asn I e Leu Asn Arg 1 5 10 Page 141 seql i st cor r ect ed. t xt lie Ala Asn u Leu Ala Leu Thr Tyr On y Val Phe 25 Ser Al a G u Thr Al a Asp Val SIle Ar g G u Pr o 145 Gy Tr p SIle G n Asn l le Leu G n Ser Al a 115 Leu Thr Gy Leu G u 195 Ser Tyr Thr Al a Leu Leu Ser G u Asp Val 165 Asp SIle Asn Phe Leu Al a SIle Ser Pr o Gy 135 I le Pr o Ser Gy Ser SIle Al a Val Tyr 105 Ser Asn Al a Tyr G u 185 I le Val Al a Gy Asn Gy SIle Ser Asp 155 Gy G u G u Leu Gy Val Gy Ser Pr o 125 Al a Val Hi s Lys Tr p 205 Al a Phe Al a Ar g Val 110 Leu Phe SIle Tyr SIle 190 Lys Ar g Al a Ser Ser G u Val Pr o Thr Leu 175 G n Ser <210> 125 <211> 1002 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(979) <223> RXA02205 <400> 125 gcccaaaccg agcctggata cccgcaaacc acttgaaccg caccacacta ctgaggtcat aaggtagtac ggtagatcgg acc ct c ct a Thr Leu Leu tgg gta caa Trp Val n ct g ccg at c Leu Pro IIe gcc gca gca gca gat cct Ala Ala Al a Ala Asp Pro acc att gtg ctc tcc atc Thr Ile Val Leu Ser Ile 30 agc tcc agc gga cac ctc Ser Ser Ser G y His Leu 45 gcc gca Al a Al a 15 gtt caa Val G n cga at c Arg Ile Page 142 accattcgct gtttcacgcc gtg aat gaa gag ata Val Asn G u G u lie 1 act gaa aat att ggc Thr G u Asn Ile G y ggc ctc aca gag ttc G y Leu Thr G u Phe atc tct gag ctg ttc IIe Ser G u Leu Phe seql i st cor r ect ed. t xt tgg ggt gcc Trp G y Al a acc gaa gcc Thr G u Al a at c aca ggt I e Thr G y gaa t ac cgc G u Tyr Arg atc ttg ggt Ile Leu G y 120 atg tgg atc Iet Trp Ile 135 ttg gcc gag Leu Al a G u 150 at g aaa gat IVet Lys Asp cct ggc gt g Pro G y Val ggt ct c aag G y Leu Lys 200 cct gca gt g Pro Al a Val 215 cca agc t cc Pro Ser Ser 230 ctg gtt gcc Leu Val Al a ttc gtg gca Phe Val Al a gca ggt ct g Al a G y Leu 280 gat gcc Asp Al a gca gt g Al a Val tgg ttc Trp Phe atg ggc et Q y 105 gtg ttg Val Leu act gca Thr Al a aag at g Lys IVet gcc at c Ala I e 170 tct cgc Ser Arg 185 cgt gaa Arg G u ctt ggc Leu G y gga caa G y G n ttc gta Phe Val 250 aac cac Asn Hi s 265 ctc gt g Leu Val gcg tcc Al a Ser 60 gtg ttt Val Phe ggc gt a G y Val at g at c 'e t Ile aag gac Lys Asp 125 gtg ct g Val Leu 140 aag aag Lys Lys atg ggt vet y ggc ggc G y G y gcc acc Al a Thr 205 ggt ttg G y Leu 220 gcc t cc Al a Ser ggc tac G y Tyr ttc agc Phe Ser ctg ct g Leu Leu ttt acc gcc gtg Phe Thr Ala Val ttt gcc aag gaa Phe Ala Lys G u ttc aat aag gaa Phe Asn Lys G u 95 att gtt gcc acc Ile Val Ala Thr 110 ctg atc cgt gag Leu lie Arg u atc ctg ttc tcc Ile Leu Phe Ser 145 gaa cgc gac tac G u Arg Asp Tyr 160 ctt gca cag tgt Leu Ala G n Cys 175 acc atc tct gct Thr Ile Ser Ala 190 aag ttc tcc ttc Lys Phe Ser Phe tac tcc ctg cct Tyr Ser Leu Pro 225 ggc cta cag ct c G y Leu G n Leu 240 att tcc att gcg Ile Ser Ile Ala 255 tgg ttt gct gca Trp Phe Ala Ala 270 ct c gca ct g gc Leu Ala Leu a y gtt Val at c SIle cgc Ar g at t SIle gcg Al a 130 ct g Leu gac Asp ctt Leu ggt G y ct g Leu 210 gac Asp acc Thr tgg Tr p t ac Tyr at g Met 290 cag G n tgg Tr p cgc Ar g ccc Pr o 115 ct g Leu gt g Val aaa Lys gcg Al a ttg Leu 195 ct g Leu gct Al a gt g Val ct g Leu cgt Ar g 275 ctt Leu caa Sn gga G y 100 gtc Val cga Ar g ttc Phe ctg Leu ctg Leu 180 ttc Phe gca Al a ttt Phe ggt G y at g Met 260 att SIe ctc aac cca Leu Asn Pro taaaattcct gtacatctta aaa <210> 126 <211> 293 <212> PRT <213> Corynebacter i um gl ut ai cum 1002 Page 143 seqI i st car r ect ed. t xt <400> 126 ValI Thr Gy le ValI (31u (31u Thr (31u Ser 145 Tyr CYS Al a Ph e Pr o 225 Le u Al a Al a (31y As n (1u (1u O3 u As n I I e Leu Thr G3 u Ser G3 u Leu Val O31n Leu I Ie Tr p O31n Ar g Ar g O31y 1 00 I Ie Pr o Val 115 Ala Leu Arg 130 Leu Val Phe Asp Lys Leu Leu Al a Leu 1 80 03 y Leu Phe 1 95 Leu Leu Al a 210 Asp Al a Phe Thr Val G31y Tr p Leu I'b t 260 Tyr Ar g Ilie 275 I'b t Leu Asn I I e Thr G31y Tr p Phe Leu Phe Tr p G31y Thr 70 li1e I Ie Phe 03 u Val I Ie As n I'b t I I e Leu 150 Thr I'b t 1 65 li1e Pr o Leu 03 y I Ie Pr o Al a Pr o 230 Thr Leu 245 Lys Phe Pr o Al a Pr o Leu Leu Al a ValI Pr o G31y 55 O31u Thr Tyr Le u Tr p 135 Al a Ly s G31y Le u Al a 215 Ser ValI ValI G31y O31n lIe 40 Al a Al a G31y Ar g G31y 120 li1e O31u As p ValI Ly s 200 ValI Ser Al a Al a Le u 280 Thr Ser As p Al a Tr p vb t 1 ValI Thr Ly s Al a Ser 1 Ar g Le u G31y Ph e As n 265 Le u Al a 10 lIe Ser Al a ValI Ph e 90 G31y Le u Al a vb t I Ie 1 70 Ar g O31u G31y O31n ValI 250 Hi s ValI Al a Al a Asp Pro ValI Ser G31y Le u 75 Al a Tr p G31y Ser G31y 1 55 li1e Ser ValI Ser Al a 235 ValI Ser vb t Le u G31y Al a ValI G31y Ly s ValI 140 Ly s G31y Al a G31y 220 Al a G31y Ph e Le u Ser Hi s Ser Ph e ValI I Ie As p 125 Le u Ly s G31y G31y Thr 205 Le u Ser Tyr Ser Le u 285 Al a ValI Ar g Thr Al a As n ValI I Ie Le u Ar g Al a 1 li1e Ph e Ser Le u Ser 255 Ph e Al a Al a O31n I Ie Al a Ly s Ly s Al a Ar g Ph e As p 1 O31n Ser Ser Le u O31n 240 li1e Al a Le u <210O> 127 <21 1> 975 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (952) Page 144 seqi i st car r ect ed. t xt <223> RXA00900 <400> 127 gtgcggtggc tgcgctggtc gctagtgtgg tgtgcgcgct tagtgatcgc acgcctggtg caggggcttg gcggcggtgc ggcgccgtcg ataagcgtat gt g cgt ggt at t gcg 11! Val Arg Gy lieAla cgc Ar g ttt Phe ct C Leu t gg Tr p cag O31n ggc G31y ttg Leu t ac Tyr gt a Val gt g Val 150 cgc Ar g ttg Leu ct g Leu aca Thr gca Al a 230 at c l Ie ct g Leu ggt 3y ct t Leu aag Lys at g lvb t t at Tyr t cg Ser 120 ct g Leu gga G31y at g at t l Ie ttt Phe 200 ct g Leu ttg Leu cca Pr o at g lvb t gt g Val ct g Leu t cg Ser t cc Ser ttc Phe t cg Ser t cc Ser gt c Val act Thr 170 gt g Val at c l Ie act Thr ttc Phe gac Asp at t l Ie ct g Leu gt g Val aag Lys 75 aac Asn aca Thr ccg Pr o at t l Ie aat Asn 155 gt g Val ct g Leu gt t Val gaa O31u gt g Val 235 ct t Leu at t l Ie gt c Val aat Asn cca Pr o t at Tyr ttg Leu ttc Phe at t l Ie 140 agg Ar g ct g Leu ttt Phe t cc Ser gt g Val 220 caa (31n g aa O31u cag O31n 9gg O31y 45 ttt Phe gt t Val gt c Val ggg O31y gt g Val 125 ttc Phe cga Ar g gcc Al a at t l Ie cat Hi s 205 gt g Val ttc Phe cgc Ar g gga O31y cct Pr o gcg Al a gaa O31u ttt Phe ct g Leu 110 ct g Leu gga O31y ct t Leu agt Ser aat Asn 190 at t l Ie cga Ar g acg Thr gga O31y 15 at t l Ie ttt Phe cag O31n gag (31u gt g Val 95 t ct Ser cag O31n gt g Val ct g Leu ttt Phe 175 t gg Tr p ccg Pr a agc Ser at g IN/bt caa O31n gct Al a ggc O31y ct g Leu cgt Ar g 80 ct g Leu ttt Phe aat Asn aat Asn cag O31n 160 act Thr at a l Ie at g lvb t agg Ar g ggt 31y 240 1 aag Lys ccc Pr a t gg Tr p ct t Leu acc Thr aag Lys ggg O31y caa O31n gct Al a 145 cgg Ar g gt g Val ccg Pr a gt t Val gcg Al a 225 gct Al a gct Al a gt g Val cgg Ar g gt t Val gca Al a aat Asn gcg Al a at g lvb t 130 ttt Phe at t l Ie ct g Leu ct g Leu at g lvb t 210 gga O31y ttg Le u gcg Al a gt a Val gga O31y gct Al a gca Al a cct Pr a at g rvb t 115 ggc O31y ggt O31y cat Hi s t gt Cys ttc Phe 195 gct Al a t cg Ser gt g Val cac Hi s gct Al a at t l Ie ttg Leu gga O31y caa O31n 100 ttc Phe at t l Ie ttg Leu cca Pr a gcg Al a 180 ct g Leu aac Asn ggt O31y agt Ser gcc Al a ccg Pr a ttc Phe ct g Leu ct t Leu ttt Phe t cc Ser ccg Pr a at t l Ie cac Hi s 165 ct t Leu ttg Leu gcg Al a t ct Ser t ca Ser 245 Page 145 ctg gtc gga Leu Val G y act gct t gt Thr Al a Cys aaa ggt att Lys G y II e 280 ggc Gy 250 ct g Leu gaa G u gat Asp gcg Al a aag Lys seql i st cor r ect ed. t xt aag gct ttg act atg gga atc gca atg Lys Ala Leu Thr IVet y Ile Ala IVet 255 260 tgt ggg tgt gcg tac ctg gca ggt cga Cys Gy Cys Ala Tyr Leu Ala Gy Arg 270 275 tagctctagg tggcgtttta agg <210> 128 <211> 284 <212> PRT <213> Corynebacteri um gl utam cum <400> 128 Val Arg Gy Ile 1 Lys Ala Ala His Pro Val Val Ala Trp Arg Gy lie Leu Val Ala Leu Thr Al a Al a G y Lys Asn Pro G n 100 Sy Ala Met Phe 115 Sn Met Gy lie 130 Al a Phe G y Leu 145 Arg Ile His Pro Val Leu Cys Al a 180 Pro Leu Phe Leu 195 Val Met Ala Asn 210 Al a y Ser G y 225 Ala Leu Val Ser Al a 5 Al a Pr o Phe Leu Leu Phe Ser Pr o SIle Hi s 165 Leu Leu Al a Ser Ser 245 Arg Ala lie Val Phe Leu Tr p G n 70 Gy Leu Ty r Val Val 150 Ar g Leu Leu Thr Al a 230 Leu Al a SIle Al a I le Gy Al a SIle Leu 135 Gy SIle Leu Leu Al a 215 l le Val Leu Gy Al a G u Leu Val 105 Al a Tyr Mbet G n G u 185 Leu Gy Gy Leu Asp SIle Leu Val Lys Asn Thr Pr o SIle Asn 155 Val Leu Val G u Val 235 G u G n Gy Phe Val Val Gy Val 125 Phe Ar g Al a SIle Hi s 205 Val Phe Gy I e Phe G n G u Val Ser G n Val Leu Phe 175 Tr p Pr o Ser bet Ser Asp Lys Ala Leu Thr 255 Page 146 seqI i st car r ect ed. t xt I'bt G31y I Ie Al a I'bt Thr Al a Cys Al a Leu Leu Al a Cys G31y Cys Al a 260 265 270 Tyr Leu Al a G31y Arg Lys G1y Ilie Pr o G31u I'bt Lys 275 280 <210O> 129 <21 1> 537 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri curn <220> <221 CIDS <222> 101). (514) <223> RXN00901 <400> 129 tt t taagt at t ggt gct at c t tccggt gct gat ggt acct aaat accccc aaat ct tcga t at agat aca cgagacagt g gaat gaaaat t tct aat taa atg cag aaa aaa caa 11! I'bt G3 n Lys Lys G3 n c ag G n t cc Ser g aa 0Gu tt t Ph e g at As p ct g Le u gt g ValI tt g Le u ac g Thr agc acc gcc Ser Thr Al a ct a gcg act Leu Al a Thr ttg ggg aca Leu G3 y Thr gct gga at g Al a G3 y [Vb t ttg gga agg Leu a31y Ar g gct agt gt g Al a Ser Val gca cgc ct g Al a Ar g Leu 1 05 gcg cga t cg Al a Ar g Ser 120 t tg cac t gc Leu Hi s Cys ct g Le u g at As p act Thr gcg Al a aaa Ly s 75 gt g ValI gt g ValI t gc Cy s at t I Ie at g vb t gca Al a at t I Ie ggc a31y t gc Cys c ag a31n c ag a31n gga G y tt g Le u 30 at g vb t c aa a n ct c Le u ct g Le u ct t Le u 110 tt g Le u gca Al a gca Al a c ag a31n at c I Ie gca Al a 80 ccg Pr o a31y gcg Al a 1 tt a Le u at g Le u at t I Ie 65 ggt a31y t cg Ser ggt a31y g ac As p tt g Le u cct Pr o act Thr 50 ggt a31y gcg Al a at a I Ie gcg Al a aaa Ly s 130 t ca Ser ggt a31y 35 ct t Le u cct Pr o gt g ValI ag c Ser t gc Cys 115 ag g Ar g gcc Al a 20 at t I Ie t ct Ser tt g Le u gct Al a gt a ValI 1 00 gt g ValI ct g Le u ag c Ser gcg Al a t cc Ser t cg Ser gcg Al a tt a Le u gt a ValI cgc Ar g t gat gat tat t cagggaat t gct <210O> 130 <21 1> 138 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 130 I'b t a31n Lys Lys a31n a31n Leu Ser Thr Al a Leu I Ie I'bt a31y Leu Al a Page 147 seqi i st car r ec 10 Leu Leu Ser A a Ser Ser A a Leu A a Thr Asp N/t Pro G31y I Ie Ala (31u Asp Leu G31y Thr Thr 40 Leu Thr Leu Ser Ser Phe l'bt Ala 31y l'b t Ala 55 li1e G31y Pr o Leu Ser As p O31n Leu G31y Ar g Lys 70 75 31y Ala Val Aia AiaLeu Val Ala Ser Val Val 90 Ser I Ie Ser Val Leu Val I Ie Ala Arg Leu Val 100 105 G31y Ala Cys Val Val Leu Ar g Ala Ar g Ser Cys 115 120 As p Lys Ar g Leu Ar g Thr Pr o Leu Hi s Cy s 130 135 <210O> 131 <21 1> 501 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> 101). (478) <223> FRXA00901 <400> 131 acct gaat ga aaat t tct aa t taaaaat ac ccccaaat ct agtgatgcag aaaaaacaac agctgagcac cgccctgatt ted. t xt l'b t Ty r Ala Pro li1e G31y 03 y Leu Cys A a (31n (1y 03 n Thr 125 Pr o ValI Le u ValI A a G31y As n A a (31n I Ie A a Pr o G31y A a tt g Le u cct Pr o act Thr ggt G31y gcg A a at a I Ie gcc A a at t I Ie t ct Ser tt g Le u gct A a gt a ValI t cc Ser g aa G31u tt t Ph e g at As p ct g Le u gt g ValI gcg A a g at As p at g vb t caa O31n gt c ValI 75 at c I Ie ct a Le u tt g Le u gct A a tt g Le u gct A a gca A a gcg A a ggg G31y gga G31y 45 gga G31y agt Ser cgc Ar g act Thr ac a Thr at g vb t ag g Ar g gt g ValI ct g Le u gat at g As p lb t 15 act gca Thr A a gcg att A a I I e aaa ggc Lys 03 y gt g t gc Val Cys 80 gt g cag Val O31n 95 Page 14E t cgat at aga t acacgagac at g gga t tg gca tt a l'b t G31y Leu Ala Leu 1 t at t tg ccg gca at g Ty r Leu Pr o Ala l'b t ccg at g gt g cag tt a Pr o l'bt Val O31n Leu ggc caa t tg at c at t 03 y 03 n Leu I I e I I e ct g ct c gt t gca ggt Leu Leu Val Ala G31y gcg ct g gcg ccg t cg Ala Leu Ala Pr o Ser ggg ct t ggc ggc ggt 03 y Leu 03 y 03 y 03 y 1 00 seqI i st car r ect ed. t xt gcg tgc gt g gt a t tg cgc gcg cga tcg tgc cag acc ttg aac gcg gac Ala Cy sVal Val Leu Ar g Aa Ar gSer Cys 31n Thr Leu Asn AlaAsp 105 110 115 aaa agg ct g cgc acg cct ttg cac t gc t gat gat tat t cagggaat t Lys Arg Leu Ar g Thr Pr o Leu Hi s Cys 120 125 gct <210> 132 <211> 126 <212> PRT <213> Cor ynebact er i urn gi ut anri cumn <400> 132 I'b t G31y Leu Ala Leu Leu Ser Ala Ser Ser Ala Leu Ala Thr Asp I'bt 1 5 10 Tyr Leu Pro Ala I'bt Pro Gy IlieAla Ou Asp Leu G31y Thr Thr Ala 25 Pr o I'bt Val O31n Leu Thr Leu Ser Ser Phe I'b t Ala 31y I'b t Ala Ilie 40 G31y O31n Leu Ilie Ilie O31y Pro Leu Ser Asp O31n Leu O31y Ar g Lys O31y 55 Leu Leu Val Ala(1y Aa Val Ala AlaLeu Val Ala Se r ValI Val Cys 70 75 Ala Leu Ala Pr o Ser Ilie Ser Val Leu Val Ilie Ala Ar g Leu Val O31n 90 O31y Leu O31y 31y O31y Ala Cys Val Val Leu Ar g Ala Ar g Ser Cys O31n 100 105 110 Thr Leu Asn Ala Asp Lys Ar g Leu Ar g Thr Pr o Leu Hi s Cys 115 120 125 <210> 133 <211> 1299 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221> CIDS <222> (1276) <223> RXA00289 <400> 133 cct ccccat a agt t cact ca agcaagt t ct cccgaacaga t t cacccgag aagt cgacag accccattaa acagcccgat tcaagaaagg cttcgcagcc atg agc acc acc acc I'b t Ser Thr Thr Thr 1 gcg ccc gaa gca cgg ttt cct gtc gtc cct t t gacc gcc at gagt tt c Ala Pr o O31u Ala Ar g Phe Pr o Val Val Pr o Leu Thr Ala I'b t Ser Phe 15 gcg gca ttt gtt t at gt c acg t tc gag at g t tt gca gt t ggc ct c at c Ala Ala Phe Val Tyr Val Thr Phe O31u I'b t Phe Ala Val O31y Leu Ilie 30 aag ccg atg gcc agc gat ctt gga gtg tca gaa tcc agc atc ggc ctg Page 149 SE Lys Pro l'bt Ala Ser Asp Leu tt g Le u at g vb t ct g Le u t at Tyr gt g ValI c ac Hi s ct g Le u 1 50 t gg Tr p gct A a g aa (31u ct g Le u ac c Thr 230 t cc Ser gt g ValI ac c Thr gga G31y act Thr t gg Tr p tt t Ph e at g vb t t gg Tr p 120 ggt G31y gt t ValI cct Pr o ct c Le u gag (31u 200 at t I Ie ct t Le u ggc G31y ac c Thr act Thr 280 gag (31u t at Tyr t ct Ser gcc A a gcc A a ct t Le u gca A a t ct Ser ac c Thr 1 70 cca Pr o aaa Ly s ct t Le u ct c Le u tt c Ph e 250 act Thr tt t Ph e ac a Thr gcg A a cga Ar g 75 ac g Thr at c I Ie gt t ValI gt a ValI ccg Pr o 155 t gg Tr p acc Thr gaa (31u ct c Le u at c I Ie 235 gcc A a gt g ValI gt c ValI ct a Le u act Thr tt t Ph e ggC G31y G31y ggg G31y G31y 140 ct g Le u at t I Ie gt t ValI aag Ly s ct t Le u 220 at c I Ie tt c Ph e g at As p at t I Ie gcc A a 300 (31y 45 gt c ValI aac As n at t I Ie cgc Ar g cca Pr o 125 gt t ValI gca A a ct t Le u cca Pr a aaa Ly s 205 gcc A a gct A a ggt G31y c aa O31n gct A a 285 gt a ValI eql i st car r ect ed. t xt Val Ser u Ser Ser I I e 03 y Leu gt t ValI aag Ly s gt t ValI act Thr 110 at g vb t gt g ValI ac a Thr ggt G31y t ca Ser 1 90 t cc Ser gt c ValI g aa (31u gca A a cgc Ar g 270 gca A a gt a ValI gcc A a cgc Ar g gt t ValI 95 at c I Ie gca A a t cg Ser t gg Tr p gcg A a 1 75 ct g Le u ct t Le u ac c Thr ac a Thr ct c Le u 255 at g vb t at t I Ie gct A a gt g ValI ac a Thr 80 c ag O31n gca A a gcg A a at t I Ie at c I Ie 1 60 ct g Le u cca Pr a cca Pr a ggt G31y gca A a 240 gga G31y ct g Le u ct c Le u at c I Ie gt g ValI gt t ValI gca A a gca A a cgt Ar g gga G31y 145 ggt G31y ac c Thr cca Pr a t gg Tr p gt t ValI 225 ggg G31y ct c Le u cgt Ar g gga G31y tt c Ph e 305 ac g Thr tt c Ph e ct g Le u tt g Le u at g vb t 130 t ca Ser g aa O31u at t I Ie ct t Le u ggt G31y 210 tt t Ph e g ac As p at t I Ie gga G31y c ag O31n 290 ct t Le u at c I Ie ct g Le u ac c Thr act Thr 115 t cc Ser ac c Thr ct c Le u gcg A a cca Pr a 1 ct c Le u gct A a ag c Ser ggc G31y agt Ser 275 at c I Ie gca A a cct Pr a at t I Ie gt t ValI 1 00 c ac Hi s cca Pr a at g vb t at c I Ie gcc A a 1 g ac As p at t I Ie gcc A a tt c Ph e gt g ValI 260 gt t ValI gca A a gt c ValI gcc A a act Thr aat As n ggg G31y ggt G31y gcg A a gga G31y 1 gt g ValI ac g Thr t cc Ser t ac Tyr gt g ValI 245 ac a Thr c ac Hi s tt c Ph e ac c Thr 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 gt g t tt ggt gga gca t ac ggc gct ct c cca acc ct g gga acc acc at c Page 150 seql i st cor r ect ed. t xt Val Phe Gy y Ala Tyr Gy Ala Leu Pro Thr Leu y 310 315 320 ttc ctc cat gcg ggt cgc gac cac cca gat act gca tcc Phe Leu His Al a G y Arg Asp His Pro Asp Thr Al a Ser 330 335 gtg gtc act tac caa gtg ggt atc gcg tct ggc gcg gca Val Val Thr Tyr G n Val Gy I e Ala Ser y Ala Al a 345 350 atg gct gtg gat gcc gat tgg gtt gct ggc act ttg tgg IVet Al a Val Asp Al a Asp Trp Val Al a G y Thr Leu Trp 360 365 370 gga ctg tca ttg gct tcc acg ttg gcc ttg gcg ctg tgg Sy Leu Ser Leu Ala Ser Thr Leu Ala Leu Ala Leu Trp 375 380 385 cta ctg aag tagcagccca aattcagccc act Leu Leu Lys 390 <210> 134 <211> 392 <212> PRT <213> Cor ynebact eri um gl ut ai cum Thr at t SIle 340 ggc Gy at g Met cgc Ar g SIe 325 t at Tyr gcg Al a gct Al a ccg Pr o 1123 1171 1219 1267 1299 <400> 134 IVet Ser Thr 1 Thr Al a Mbet Ala Val y Ser Ser Il e Val Thr Il e Val Phe Leu Ala Leu Thr Ala Leu Thr 115 Arg IVet Ser 130 G y Ser Thr 145 G y G u Leu Thr Ile Al a Pro Leu Pro 195 Thr Thr Ala Pro 5 Ser Phe Al a Al a Leu Ile Lys Pro G y Leu Leu IVet Pro Ala IVet Leu 70 Ile Thr Leu Ala Val Asn Tyr y 100 His G y Val Phe Pro G y His Thr 135 bet Al a Leu Val 150 lie y Trp Arg 165 Ala Val Ala Val 180 Asp Thr G u Ser Al a Val Al a Val Val Leu Leu 105 Al a Ar g Gy Al a SIle 185 Ser Ar g 10 Tyr Ser Tyr Ser Al a 90 Al a Leu Al a Ser Thr 170 Pr o Phe Val Asp Al a Ar g Thr l Ie Val Val Pr o 155 Tr p Thr Val Phe Gy Val Asn SIle Ar g Pr o 125 Val Al a Leu Pr o Pr o bet Ser Al a Ar g Val I e Al a Ser Tr p Al a 175 Leu Lys G u Lys Lys Ser Leu Pro 205 Page 151 seql i st cor r ect ed. t xt Trp y 210 Val Phe 225 Sy Asp Leu II e Arg G y G y G n 290 Phe Leu 305 Leu G y Al a Ser Al a Al a Leu Trp 370 Leu Trp 385 Leu Ile Ser Leu Val lie Phe Leu Leu Leu Ala Val Thr y 215 220 Al a Ser Gy Ser 275 I le Al a Thr Ser Leu 355 I le Ser Tyr Val 245 Thr Hi s Phe Thr SIle 325 Tyr Al a Al a Pr o Tyr SIle Al a Thr Leu 295 Phe Leu Val Al a Leu 375 Leu Gy Leu Ar g 265 Leu Gy Gy Al a Tyr 345 Asp Leu Leu Phe 250 Thr Phe Thr Al a Gy 330 G n Al a Al a SIle 235 Al a Val Val Leu Tyr 315 Ar g Val Asp Ser Al a Gy G n Al a 285 Val Al a Hi s SIle Val 365 Leu G u Al a Ar g 270 Al a Val Leu Pr o Al a 350 Al a Al a Thr Leu 255 bet SIe Al a Pr o Asp 335 Ser Gy Leu <210> 135 <211> 420 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(397) <223> RXN01984 <400> 135 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa caatagtgcc ggtgagggag ctgtccgata ttgtgct t ac aaa Lys ggg Gy gaa gtu ggt cct Pr o at c SIle t cc Ser ttc aag Lys gga Gy gtt Val at c gt c Val at c SIle cga Ar g gt g gac Asp aaa Lys t at Tyr 45 acg tcg Ser cgc Ar g at c SIle gaa cag gca G n Al a 15 cgc ccg Arg Pro gt c at g Val MVet ttt gtc Page 152 gtacgcacat ttgttgtctg atg cac gaa tct gga Vet His G u Ser G y 1 cca caa gga cgc ggt Pro n Gy Arg y att cct agg caa acg lie Pro Arg G n Thr act gcc ctc gca ctc Thr Ala Leu Ala Leu tcc atg ggt ctg ctc seql i st cor r ect ed. t xt Gy y Phe Ala I e y Val Thr G u Phe Val Ser Met G y Leu Leu 60 agc gcg atc gcc tcc gac ttt gag atc tcc gaa gac caa gcc gga cac Ser Al a lie Al a Ser Asp Phe G u I I e Ser G u Asp G n Al a y His 75 80 atc atc acc atc tac gcc ctc gcg tgg ttg tgg gtg ccc cgc lie lie Thr Ile Tyr Ala Leu Ala Trp Leu Trp Val Pro Arg tgatcacagc gtttaccggc aaa <210> 136 <211> 99 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 136 Met Hi s u Ser G y Lys Asn Pro Val Lys Val Val Asp Ser G n Ala 1 5 10 Pro n G y Arg G y G y His lie G y G y His lie Lys Arg Arg Pro 25 Ile Pro Arg G n Thr G u I e Ser G u Val Arg Arg Tyr Ile Val IVet 40 Thr Ala Leu Ala Leu y y Phe Ala Ile y Val Thr u Phe Val 55 Ser IVet y Leu Leu Ser Ala Ile Ala Ser Asp Phe G u Ile Ser G u 70 75 Asp G n Ala G y His lie lie Thr IIe Tyr Ala Leu Ala Trp Leu Trp 90 Val Pro Arg <210> 137 <211> 379 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(379) <223> FRXA01984 <400> 137 aggaaatgtc tcacgtcaca accttttgaa aggtggctaa caatagtgcc ggtgagggag ctgtccgata ttgtgct t ac aaa aat cct gtc aag gtt gtc gac Lys Asn Pro Val Lys Val Val Asp ggg cat atc ggc gga cat atc aaa G y His i e G y G y Hi s i e Lys gaa att tcc gag gtt cgt cga tat tcg cag gca Ser G n Al a 15 cgc cgc ccg Arg Arg Pro 30 at c gt c at g Page 153 gtacgcacat ttgttgtctg atg cac gaa tct gga 115 Vet His G u Ser G y 1 cca caa gga cgc ggt 163 Pro n y Arg y att cct agg caa acg 211 lie Pro Arg G n Thr act gcc ctc gca ctc 259 G u I e Ser ggt ggc ttc G y G y Phe agc gcg atc Ser Al a II e at c at c acc Ile Ile Thr Val at c SIle t cc Ser t ac Tyr Ar g gt g Val 60 ttt Phe ct c Leu seql i st cor r ect ed. t xt Tyr Ile Val IVet Thr Ala Leu Ala Leu 45 acg gaa ttt gtc tcc atg ggt ctg ctc Thr G u Phe Val Ser IVet G y Leu Leu gag atc tcc gaa gac caa gcc gga cac G u I I e Ser G u Asp G n Al a G y Hi s 80 gcg Al a <210> 138 <211> 93 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 138 IVet His G u Ser G y Lys Asn Pro Val 1 5 Pro Gn Gy Arg Gy Gy His lie G y 25 lie Pro Arg n Thr u Ile Ser u 40 Thr Al a Leu Al a Leu G y G y Phe Al a Ser IVet y Leu Leu Ser Ala I e Al a 70 Asp G n Al a y His li e li e Thr I I e Val Hi s Ar g Sy Asp 75 Al a Asp Lys Tyr Thr a u Al a Ser G n Al a Arg Arg Pro Ile Val Met G u Phe Val Ile Ser G u <210> 139 <211> 735 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(712) <223> RXA00109 <400> 139 aagtggggga agatttcgac aactaaccgg gcgcaaagat gaaactaatg cgtccgacca cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gtg gct tca gag aag Val Ala Ser G u Lys 1 aat cta aaa ttg cgt acc ttg gcg gca gct gct ggg gtg ttg ggc gtt Asn Leu Lys Leu Arg Thr Leu Ala Ala Ala Ala y Val Leu y Val 15 ggc gcg atg tcg atg ctc gtg gct ccg cag gct gct gcc cat gat gtg G y Al a Met Ser Met Leu Val Al a Pro G n Al a Al a Al a Hi s Asp Val 30 gtg gtg gat tct aat cct gaa aat ggc agt gtc gtt gat gag ttc ccg Val Val Asp Ser Asn Pro G u Asn y Ser Val Val Asp G u Phe Pro Page 154 seql i st cor r ect ed. t xt gag acc att gag ttg gag ttt Su Thr Ile u Leu u Phe 60 aca gtt gca ttg agc aat gcg Thr Val Ala Leu Ser Asn Ala 75 act cct cag ctt gag ggg cag Thr Pro n Leu u Gy n gtg cag acg gga gct ggt aac Val n Thr y Al a y Asn 105 t ct gat ggt cac gct act aaa Ser Asp G y His Ala Thr Lys 120 tct gct gaa acg aca aca gag Ser Ala G u Thr Thr Thr G u 135 140 gca gca acc act gac acc tca Ala Ala Thr Thr Asp Thr Ser 150 155 act gct gat gaa act t ct gga Thr Al a Asp G u Thr Ser G y 170 agc atc gtg gcg gtg ctt gtt Ser lie Val Ala Val Leu Val 185 gca aag aat cgt aac cag aaa Ala Lys Asn Arg Asn G n Lys 200 <210> 140 <211> 204 <212> PRT <213> Cor ynebact er i um gl ut a <400> 140 Val Ala Ser G u Lys Asn Leu 1 5 Sy Val Leu y Val G y Ala Ala Ala His Asp Val Val Val Val Asp G u Phe Pro G u Thr 55 G n Asp Leu Phe Thr Thr Val 70 Val Leu Thr Ser y Thr Pro G u Val Pro Ser Asp Val G n ggt Gy t cc Ser ttg Leu at t SIle 110 t ca Ser aca Thr acc Thr cct Pr o gca Al a 190 at t SIle gga Gy agc Ser 95 ttg Leu at c SIle gca Al a acc Thr gcg Al a 175 agt Ser cct Pr o gag G u 80 t at Tyr ggt Gy tct Ser gag G u gaa G u 160 ccg Pr o gcc Al a cag gat Sn Asp gtg tta Val Leu gaa gt g G u Val ttc cag Phe G n ttt gag Phe G u 130 acg aca Thr Thr 145 gca gag Al a G u tgg aat Trp Asn at c gt c I e Val ttc Phe tct Ser tct Ser 100 act Thr aca Thr gag G u act Thr gtt Val 180 at g Met aca Thr gga Sy gat Asp tct Ser ggc Sy t ca Ser gaa G u 165 ttg Leu att SIe taagagggtt tattcaccat gaa ri cum Lys Leu bet Ser 25 Asp Ser 40 Ile G u Al a Leu n Leu Thr G y Arg Thr Leu 10 bet Leu Val Asn Pro u Leu G u Phe Ser Asn Al a 75 Su y n 90 Al a G y Asn Page 155 Al a Al a Asn Ser Asp Hi s Tyr Al a Pr o Sy Sy Ser Leu SIle Al a G n Ser SIle Sy Ser Leu Al a Al a Val Pr o G u Tyr Sy Phe Phe Thr 145 Al a Tr p SIle SIle 115 Val Thr Thr Tr p Met 195 Ser Gy Ser G u 165 Leu SI e Ser Ser Al a 150 Thr Ser Al a seql i st cor r ect ed. t xt 105 Sy His Ala Thr Lys G y 120 125 G u Thr Thr Thr G u Thr 140 Thr Thr Asp Thr Ser G u 155 Asp G u Thr Ser G y lie 170 Val Al a Val Leu Val Val 185 Asn Arg Asn n Lys 200 110 Ser Thr Thr Pr o Al a 190 Ile Ser Al a u Thr G u 160 Ala Pro 175 Ser Al a <210> 141 <211> 735 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(712) <223> RXA00109 <400> 141 aagtggggga agatttcgac aactaaccgg gcgcaaagat cggcgaaaag gaagtttcgc ccatctatga gaggttgaat gaaactaatg cgtccgacca gtg gct Val Ala aat Asn ggc Gy gt g Val gag G u aca Thr act Thr gt g Val tct Ser aaa Lys at g Met gat Asp at t SIle gca Al a cag G n acg Thr ggt Gy cgt Ar g at g Met aat Asn ttg Leu agc Ser gag G u gct Al a gct Al a ttg Leu gt g Val gaa G u ttt Phe gcg Al a cag G n aac Asn aaa Lys gcg Al a gct Al a aat Asn 45 t cc Ser gat Asp cac Hi s t ac Tyr ggt Gy 1 gca gct gct ggg Al a Al a Al a G y 15 ccg cag gct gct Pro G n Ala Ala ggc agt gtc gtt G y Ser Val Val ggt att cct cag Sy lie Pro n tcc gga gag gtg Ser G y G u Val 80 ttg agc tat gaa Leu Ser Tyr G u 95 att ttg ggt ttc Ile Leu G y Phe 110 tca atc tct ttt Ser lie Ser Phe Page 156 gt g Val gcc Al a gat Asp gat Asp tta Leu gt g Val cag G n tca gag aag Ser G u Lys ttg ggc gtt Leu G y Val cat gat gtg Hi s Asp Val gag ttc ccg G u Phe Pro ctg ttc aca Leu Phe Thr act t ct gga Thr Ser G y cca t ct gat Pro Ser Asp 100 atc act tct I e Thr Ser 115 gag gtg aca ggc G u Val Thr y tct Ser gca Al a 150 act Thr agc Ser gca Al a 120 gaa G u acc Thr gat Asp gt g Val aat Asn 200 aca Thr gac Asp act Thr 170 gt g Val aac Asn aca Thr acc Thr 155 tct Ser ctt Leu cag G n gag G u 140 t ca Ser gga Gy gtt Val aaa Lys seql i st cor r ect ed. t xt 125 130 aca aca gca gag acg aca act Thr Thr Ala G u Thr Thr Thr 145 gag acc acc gaa gca gag aca G u Thr Thr G u Ala G u Thr 160 att cct gcg ccg tgg aat tgg lie Pro Ala Pro Trp Asn Trp 175 gtt gca agt gcc atc gtc atg Val Ala Ser Ala IIe Val Mbet 190 195 taagagggtt tattcaccat gaa gag t ca Su Ser act gaa Thr G u 165 gtt ttg Val Leu 180 atg att 'e t lie <210> 142 <211> 204 <212> PRT <213> Corynebacter i um gl utam cum <400> 142 Val Al a Ser G u Lys Asn Leu Lys Leu 1 Gy Al a Val G n Val G u Phe Phe Thr 145 Al a Tr p Leu Hi s G u Leu Thr Pr o SIle 115 Val Thr Thr Tr p 5 Val Val Pr o Thr Gy Asp Ser Gy Ser G u 165 Leu Al a Val Thr Val Pr o G n Asp Al a 135 Al a Al a SIle bet Asp 40 I le Al a G n Thr Gy 120 G u Thr Asp Val Ser Ser G u Leu Leu Gy 105 Hi s Thr Thr G u Al a 185 Ar g 10 bet Asn Leu Ser G u 90 Al a Al a Thr Asp Thr 170 Val Thr Leu Pr o G u Asn Gy Gy Thr Thr Thr 155 Ser Leu Leu Val G u Phe Al a G n Asn Lys G u 140 Ser Gy Val Al a Al a Asn Ser Asp Hi s Tyr Gy 125 Thr Su I e Val Al a Pr o Gy Gy Ser Leu SIle 110 Ser Thr Thr Pr o Al a 190 Al a G n Ser SIe Sy Ser Leu SIe Al a Thr Al a 175 Ser lie Val IVet IVet lie Ala Lys Asn Arg Asn n Lys Page 157 seql i st cor r ect ed. t xt <210> <211> <212> <213> <220> <221> <222> <223> 143 864 DNA Cor ynebact eri um gl ut am cum CDS (101) (841) RXA00996 <400> 143 atcttaagcc ttttgtccaa ggctctgagt tcttagtggc acctctatct tgcacctgat ctggcgtaga ctcataagtt ggtatttacc aagaagtaac agc acc gta Ser Thr Val gca gtg cag gtc Ala Val n Val aac Asn ggc cta aaa gtt G y Leu Lys Val ata tcg tcc ggt I e Ser Ser G y ttt tca Phe Ser cgc aag aaa Arg Lys Lys acc gga aag Thr G y Lys aaa acg atc ttg Lys Thr Ile Leu cat Hi s gat ctc gat ttc Asp Leu Asp Phe acc gta gag Thr Val G u ggc aag aca G y Lys Thr atc acg gga ttg I e Thr G y Leu ggg cca tcg ggc Sy Pro Ser y agc Ser act ttg Thr Leu atg cgc gcg att IVet Arg Ala lle gt g Val gga gtg caa aac Sy Val n Asn gac ggc acc ctt Asp y Thr Leu gag Gu gtg ttt gat cag Val Phe Asp G n gca ggt gct gcc Ala y Ala Al a ctg cgc ggg aaa Leu Arg Gy Lys ggc tat gtc acc G y Tyr Val Thr caa Sn aac gcc agc gta Asn Ala Ser Val t at Tyr 95 cac gat ctg tcg His Asp Leu Ser gtg at a Val lie 100 gaa aac ctc G u Asn Leu cgc acc ccg Arg Thr Pro 120 tat ttc ggg gct Tyr Phe y Al a gct aaa gga acc Al a Lys G y Thr t cc act cca Ser Thr Pro 115 gat ctt gct Asp Leu Al a gaa aag att ctg G u Lys Ile Leu gtc tta gac atc Val Leu Asp Ile gca Al a 130 caa cgc G n Ar g 135 caa gt a t ca aca G n Val Ser Thr tgt gcg ctt att Cys Ala Leu II e 155 ct a Leu 140 tct ggt ggg cag Ser G y G y G n ggc cga gtc tcc Sy Arg Val Ser ctt Leu 150 gga Sy gcc tca cca gaa Ala Ser Pro u ttg gtg atg gat Leu Val IVet Asp cca acc gtg ggt Pro Thr Val y ttg gat ccc att acc cgg caa gca ctg tgg gaa gag Leu Asp Pro lie Thr Arg n Ala Leu Trp G u G u 170 175 180 ttc acc acc Phe Thr Thr gca aaa gca ggt Ala Lys Al a y gct Al a 190 gga gtg gtt atc Oy Val Val lle t cc agt cac Ser Ser Hi s 195 gtg ttg gag gaa gcc gcg cgg tgc gac Val Leu G u G u Ala Ala Arg Cys Asp aac ctc att ttg ttg cgt gat Asn Leu lie Leu Leu Arg Asp Page 158 seqI i st car r ect ed. t xt 200 205 210 ggt cgg at c at c t gg agg gga aca ccc aca cgc ct t ct a gaa gat aca G31y Ar g I Ie I Ie Tr p Ar g G31y Thr Pr o Thr Ar g Leu Leu G31u Asp Thr 215 220 225 ggc aaa agc tca tac gaa gat gct tt ct t ggct gcc att gac ggg gt a G3 y Lys Ser Se r Ty r G3 u Asp Al a Phe Leu Al a Al a I I e Asp G3 y Val 230 235 240 245 agg t ca t gaaccct ca ct at ct gct t gcc Ar g Ser <210O> 144 <21 1> 247 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 144 I'bt Ser Thr Ser Ser G3 y Asp Phe Thr a3 y Ser a3 y Phe Asp a3 y Leu Ar g a31y Asp Leu Ser a3 y Thr Ser 115 Il e Al a Asp 130 Ar g a31y Ar g 145 Leu Val I'b t Ala Leu Trp Val Ilie Ser 1 95 Il e Leu Leu 210 Leu Leu a3 u 225 Al a Il e Asp Val Thr Al a Val Phe Ser Ar g Lys Val a31u Thr a31y Lys Thr Thr Leu 55 Thr Leu a3 u Val 70 Lys I I e a3 y Ty r Val I Ie a31u As n 1 00 Thr Pr o Ar g Thr Leu Ala a31n Ar g 135 Val Ser Leu a3 y 150 Asp a31u Pr o Thr 1 65 a3 u a3 u Phe Thr 1 80 Ser Hi s Val Leu Ar g Asp a31y Ar g 215 As p Thr a3 y Lys 230 (3y Val Ar g Ser 245 ValI Thr lie Ar g As p Thr Ly s 1 a31u ValI Al a ay le 1 au le Ser As n 10 Ly s Thr Al a a31n a31n 90 Tyr Ly s Ser Le u Le u 1 70 Al a Al a Tr p Tyr a31y Thr a31y I Ie Pr o 75 As n Ph e I Ie Thr I Ie 1 55 As p Ly s Al a Ar g a31u 235 Le u I Ie Le u ValI Al a Al a a31y Le u Le u 140 Al a Pr o Al a Ar g a31y 220 As p Ly s Le u Le u a31y a31y Ser Al a a31u 125 Ser Ser I Ie a31y cys 205 Thr Al a Ser As p Pr o a31n Al a Tyr Al a Le u a31y a31u Ar g 1 a31y As n Thr Le u I Ie Le u Ser As n Ser Hi s Ly s As p a31n Le u 1 a31n ValI Le u Ar g Al a 240 Page 159 seqi i st car r ect ed. t xt <210O> 145 <21 1> 2463 <21 2> DNA <21 3> COr ynebact er i urn gi ut arr cumn <220> <221 CDS <222> (2440) <223> RXN00829 <400> 145 t gtt t tagcc at ggacccca t act agggag agtt t tgtt t caagcgcgaa caggcct at g caaacggt ac gat at gacac t ggt gct aga aaaggt tcac at g caa aaa gct l'bt 03 n Lys A a t cc Ser aat As n gt g ValI g aa (31u tt c Ph e ac c Th r t ct Ser tt t Ph e tt c Ph e ggc G31y 1 50 at g vb t aaa Ly s g at As p t cg Ser gga G31y cgc Ar g ccg Pr o gcg A a gt g ValI cga Ar g 120 gt c ValI aac As n cca Pr o ct t Le u at t lIe cgc Ar g ggc G31y Le u at g vb t at c lIe ac c Th r gcg A a t ct Ser aag Ly s t gc cys 1 70 g ac As p t cg Ser at c I e aag Ly s at c I e gca A a 75 gt c ValI gca A a gaa (31u gt t ValI Ar g 155 gag (31u gcc A a gt c ValI cct Pr o t cc Ser aac As n ag g Ar g g at As p act Th r cct Pr o t cc Ser 140 gag (31u G31y t cc Ser c ac Hi s aaa Ly s t cg Ser 45 g aa (31u ccc Pr a c ag O31n g at As p aac As n 125 gca A a aaa Ly s at g vb t ct c Le u ggt G31y ag g Ar g ct g Le u ac c Th r g at As p gag G31u gcc A a 110 gcg A a t cc Ser gct A a ggc G31y t cc Ser 1 90 gcg A a 15 cgt Ar g gt g ValI t at Tyr gt t ValI c ag O31n 95 ac c Th r ggt G31y G31y ac c Th r ag g Ar g 1 75 ct c Le u aat As n ct c Le u tt c Ph e ag c Ser g ac As p 80 at g vb t gcg A a ggc G31y gcc A a tt c Ph e 1 60 gcc A a aac As n 1 g aa O31u ac c Th r ggc G31y act Th r cat Hi s ggc G31y at g vb t ccg Pr a at c I Ie 145 aaa Ly s t ca Ser g ac As p aac As n gt g ValI ac a Th r tt t Ph e tt g Le u gca A a tt g Le u gga G31y 130 ac g Th r cgc Ar g g ac As p ggc G31y aac As n tt c Ph e at t I Ie gt g ValI g aa O31u aac As n cgc Ar g 115 gct A a gt g ValI ac g Th r at c I Ie gcc A a 1 ct c Le u ac g Th r gct A a c aa O31n G31y cca Pr a 1 00 at t I Ie t at Ty r g aa (31u ggt G31y g ac As p 1 ct g Le u g at As p aaa Ly s ggt G31y gcg A a ggt G31y at c I Ie cgg Ar g tt g Le u t cc Ser aag Ly s ggc G31y 1 ct c Le u ac c Th r at c ccc ggt t ac acc cca ggt gga t gg agt tat cgg atg tat tca gaa Page 160 INDseql i st cor r ect ed. t xt Ile Pro G y Tyr Thr Pro G y G y Trp Ser Tyr Arg 'b t Tyr Ser u 200 205 210 tcg ggc ctt ttt gat gct gcc aag ccg att aag gat ttc acc gag gaa 787 SSer y Leu Phe Asp Ala Ala Lys Pro Ile Lys Asp Phe Thr u u 215 220 225 gaa cgc cac aac ttc ctt tat ctt gag ccc acc aag atg aag atc gct 835 O3 u Arg His Asn Phe Leu Tyr Leu u Pro Thr Lys 'b t Lys Ile Ala 230 235 240 245 ggc atc aac atg acc tat gag ggt ctt atc ccc cgc att cag aaa tcc 883 G y Ile Asn 'b t Thr Tyr u G y Leu Ile Pro Arg Ile n Lys Ser 250 255 260 00 Satg ttg tct aag gat cgc gaa ggc atg cag aag cat att cgt gcg ttc 931 L't Leu Ser Lys Asp Arg 3 u G y 'b t O n Lys His Ile Arg Ala Phe ri 265 270 275 Sgtg gat cga gcg gtt acc ttc att cct tgc cct gcg tgt ggt gga act 979 S Val Asp Arg Ala Val Thr Phe Ile Pro Cys Pro Ala Cys y y Thr r1 280 285 290 cga tta gcg cca cat gcc ttg gag tcc aag atc aat ggc aaa aac atc 1027 Arg Leu Ala Pro His Ala Leu u Ser Lys Ile Asn G y Lys Asn Ile 295 300 305 gct gag ttg tgc gcg atg gag gtc cgt gat ttg gcc aag tgg atc aaa 1075 Ala u Leu Cys Ala I'bt u Val Arg Asp Leu Ala Lys Trp Ile Lys 310 315 320 325 acg gtg gaa gcc ccc tcg gtt gct ccc ctg ctc acc gca ctg act gaa 1123 Thr Val u Ala Pro Ser Val Ala Pro Leu Leu Thr Ala Leu Thr 3 u 330 335 340 acc ctg gat aac ttc gtg gag atc ggt ttg ggc tat atc caa ctc gat 1171 Thr Leu Asp Asn Phe Val u Ile G y Leu Gy Tyr Ile n Leu Asp 345 350 355 cgc ccc gct ggc acg ttg tct ggt ggt gag gca cag cgc acc aag atg 1219 Arg Pro Ala G y Thr Leu Ser G y G y 3 u Ala O n Arg Thr Lys 'b t 360 365 370 atc cgc cat ttg ggc tct gca ttg act gac gtc acc tat gtt ttt gat 1267 Ile Arg His Leu Gy Ser Ala Leu Thr Asp Val Thr Tyr Val Phe Asp 375 380 385 gaa ccc acc gcc ggt ttg cac gcc tac gac att gaa cgc atg aac aag 1315 3 u Pro Thr Ala G y Leu His Ala Tyr Asp Ile 3 u Arg 'b t Asn Lys 390 395 400 405 ttg ctg ctc gat ctt cgc gat aaa ggc aat acc gtt tta gtc gtg gag 1363 Leu Leu Leu Asp Leu Arg Asp Lys 0 y Asn Thr Val Leu Val Val 0 u 410 415 420 cac aag ccg gaa acc atc gcc att gca gat cat gtg gtg gac ctt ggg 1411 His Lys Pro 3 u Thr Ile Ala lie Ala Asp His Val Val Asp Leu G y 425 430 435 cca ggt gca ggc gcg ggt gga ggt gaa att cgg ttt gag ggg agc gtc 1459 Pro 0 y Al a 0 y Al a 0 y 0 y 0 y 0 u Ile Arg Phe 0 u 0 y Ser Val 440 445 450 gac aag ctt aaa gac agc gac acc gtg act ggc ctc cat ttt aat gac 1507 Asp Lys Leu Lys Asp Ser Asp Thr Val Thr 0 y Leu His Phe Asn Asp 455 460 465 cgg gcg tca ttg aag gaa tcc gtg cgt gcg ccg cat ggc gcc ctg gag 1555 Page 161 Ar g 470 at c I Ie ccg Pr o t cc Ser g at As p ac a Thr 550 gt g ValI t gt cys gt a ValI gt g ValI ct g Le u 630 at t G31y cgc Ar g tt t Ph e ac c Thr 710 at c I Ie A a Ser Leu Lys G3 u Ser 475 seqi i st car r ect ed. t xt Val Arg Ala Pro His Gy A a Leu G3 u ggg G31y G31y tt g Le u 520 ac c Thr at g vb t ccg Pr o G31y t cg Ser 600 g ac As p gct A a cct Pr o at c I Ie cgt Ar g 680 tt g Le u ct g Le u at c I Ie g at As p 490 tt c Ph e cat Hi s at c I Ie g at As p ct g Le u 570 ggc G31y t gt cys c ac Hi s aat As n gca A a 650 ct c Le u aag Lys5 gag (31u ct t Le u c ac Hi s 730 cga Ar g ac g Thr gag (31u cac Hi s t cg Ser 555 t t c Ph e t cg Ser gag (31u tt t Ph e gcg A a 635 aag Lys5 99c G31y ct c Le u ccc Pr o tt t Ph e 71 5 cac Hi s aat As n gcg A a at t I Ie ggt G31y 540 at t I Ie t cc Ser gt c ValI gt g ValI ggt G31y 620 t at Tyr at c I Ie c ag O31n gcc A a ac c Thr 700 g at As p ct c Le u aat As n at t I Ie ccg Pr a 525 t ct Ser cgc Ar g ccc Pr a t at Tyr t gc cys 605 99c G31y gag G31u gca A a ccg Pr a ac c Thr 685 ac a Thr c aa O31n G31y tt g Le u t cc Ser 510 cgt Ar g aat As n aag Lys5 aat As n gt c ValI 590 gag O31u aag Lys5 tt t Ph e aag Lys5 ct c Le u 670 c ac Hi s ggc G31y ct g Le u gt g ValI 480 aat As n gt t ValI gag O31u t cc Ser tt t Ph e 560 g aa O31u tt g Le u aag Ly s at c I Ie gcg A a 640 ct t Le u ac g Thr gca A a c ac Hi s g at As p 720 gct A a gt g ValI gca A a t cg Ser aat As n 545 gcc A a ggc G31y G31y cgt Ar g gca A a 625 gcg A a gt c ValI tt g Le u g ac As p ct c Le u 705 g ac As p c ac Hi s g at As p ggt G31y gt t ValI 530 cct Pr a aag Ly s gcg A a at g vb t tt t Ph e 610 g ac As p aaa Ly s g ac As p t cc Ser aag Ly s 690 gct A a ggc G31y gct A a gt c ValI t cg Ser 515 gt g ValI gcg A a gcc A a t gc cys at g vb t 595 g at As p gt g ValI g at As p gt c ValI ggc G31y 675 gcc A a g at As p aag Ly s g ac As p g at As p 500 ggt G31y Ph e ac a Thr aat As n cca Pr a 580 gct A a gag O31u tt g Le u t ca Ser ggc G31y 660 ggt G31y ac c Thr gt g ValI t ct Ser c ac Hi s 740 485 at t I Ie aag Ly s gt c ValI t at Tyr g at As p 565 aac As n ggg G31y t cc Ser ggg G31y aag Ly s 645 ct c Le u g aa O31u ac c Thr aaa Ly s gt c ValI 725 at c I Ie 1 603 1651 1 699 1 747 1 795 1 843 1891 1 939 1 987 2035 2083 2131 2179 2227 2275 2323 2371 at t gat gt c ggc cct ggt gca ggt t ct gat ggt ggc t cg at t gt a tt c Page 162 I e Asp Val y Pro Gy Ala G y 745 7 gag ggc agc ccc gcg gaa ctc atc a Gu Gy Ser Pro Ala G u Leu I e L 760 765 cac ctt aaa gct tat gta gat tagtt His Leu Lys Ala Tyr Val Asp 775 780 <210> 146 <211> 780 <212> PRT <213> Cor ynebact eri um gl ut ari cum 1 i st cor r ect ed. t xt ier Asp Gy y Ser Ile Val Phe '50 755 iaa act gat act cca aca gga cgc .ys Thr Asp Thr Pro Thr Gy Arg 770 tctta tggaaaaccc tgg 2419 2463 <400> 146 IVet G n Lys Ala 1 G u Thr Gy Thr Hi s Gy Met Pr o SIle 145 Lys Ser Asp Ar g Asp 225 Lys Ar g Asn Phe I e Val G u Asn Ar g 115 Al a Val Thr SIle Al a 195 Tyr Thr Lys G n Asp Ser 5 Lys Asn G y Val Al a u G y Phe 70 lie Thr Arg Ser Leu Phe Ser Phe Lys G y 150 G y Met 165 Leu Lys Thr Ile Su Ser G u G u 230 Al a G y 245 Ser Met Hi s Val Ser Ser Met Thr Thr Ser Asn 135 Sy Cys G u Pr o Sy 215 Ar g SIle Leu Tr p Val Ser Ar g Ser SIle Sy 105 I le Pr o Thr Al a Phe 185 Tyr Phe Asn bet Lys 265 Ser SIle Lys SIle Al a 75 Val Al a G u Val Ar g 155 G u Al a Pr o Al a Leu 235 Tyr Val Pr o Ser Asn Ar g Asp Thr Pr o Ser 140 G u Sy Ser Sy Al a 220 Tyr G u Hi s Lys Ser G u Pr o G n Asp Asn 125 Al a Lys Met Leu Sy 205 Lys Leu Sy Sy Ar g Leu Thr Asp G u Al a 110 Al a Ser Al a Sy Ser 190 Tr p Pr o G u Leu Al a Ar g Val Tyr Val G n Thr Sy Sy Thr Ar g 175 Leu Ser SIle Pr o SIle 255 Asn Leu Phe Ser Asp bet Al a Sy Al a Phe 160 Al a Asn Tyr Lys Thr 240 Pr o Asp Arg G u G y Iet Gn Lys 270 Page 163 seqI i st car r ect ed. t xt His IlieArg Ala Phe Val Asp Arg 275 280 Al a Val Thr Phe Ilie Pro Cys Pro 285 Al a As n 305 Al a Thr Tyr O31n Thr 385 G u ValI ValI Ph e Le u 465 Hi s ValI Al a Ser As n 545 Al a G31y G31y Ar g G31y Lys5 Tr p Le u O31n 355 Thr ValI vb t ValI As p 435 G31y Ph e Al a ValI Ser 515 ValI Al a Al a Cys vb t 595 As p Thr I Ie Lys5 325 O31u As p vb t As p Ly s 405 O31u G31y ValI As p O31u 485 li1e Lys5 ValI Tyr As p 565 As n G31y Ser Ar g Al a 310 Thr Thr Ar g I Ie O31u 390 Le u Hi s Pr o As p Ar g 470 li1e Pr o Ser As p Thr 550 ValI Cy s ValI ValI Le u 295 O31u ValI Le u Pr o Ar g 375 Pr o Le u Lys5 G31y Lys5 455 Al a Ar g Le u Ser O31n 535 G31y Ly s Ly s Ser Le u 615 Al a Le u (31u As p Al a 360 Hi s Thr Le u Pr a Al a 440 Le u Ser G31y G31y Le u 520 Thr vb t Pr a G31y Ser 600 As p Pr a Cys Al a As n 345 G31y Le u Al a As p (31u 425 G31y Ly s Le u Al a ValI 505 li1e Al a Le u Al a Al a 585 Pr a Tyr Al a vb t 315 Ser ValI Le u Ser Le u 395 Ar g I Ie G31y Ser (31u 475 Ar g Thr (31u Hi s Ser 555 Ph e Ser (31u Ph e Le u 300 (31u ValI (31u Ser Al a 380 Hi s As p Al a G31y As p 460 Ser As n Al a I Ie G31y 540 li1e Ser ValI ValI G31y 620 (31u ValI Al a I Ie G31y 365 Le u Al a Ly s G31y 445 Thr ValI As n I Ie Pr a 525 Ser Ar g Pr a Tyr Cys 605 G31y Ly s As p Le u 335 Le u (31u As p As p As n 415 As p I Ie Thr Al a As n 495 G31y As p Ar g Al a Se r 575 As p G31y As p Al a Asp Val Leu G31y Leu Ser Al a Al a Asn Al a Tyr O31u Phe Phe Al a Page 164 sec 625 630 Ala Lys Asp Ser Lys I e Leu Pro A 645 Val Asp Val G y Leu G y Tyr Ile T 660 6 Leu Ser G y G y G u Arg n Arg L 675 680 Asp Lys Ala Thr Thr Phe Ile Leu A 690 695 Leu Ala Asp Val Lys Thr Leu Leu A 705 710 Asp y Lys Ser Val Ile Val Ile C 725 His Ala Asp His Ile Ile Asp Val C 740 7 Sy Ser Ile Val Phe Gu y Ser F 755 760 Thr Pro Thr G y Arg His Leu Lys A 770 775 <210> 147 <211> 278 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA00829 <400> 147 ttg gat gag ccc acc aca ggc ctg c Leu Asp G u Pro Thr Thr y Leu I 1 5 ctg gat ctt ttt gat caa ctg gtt g Leu Asp Leu Phe Asp G n Leu Val A atc gaa cac cac ctc ggc gtg ctc g lie G u His His Leu G y Val Leu 40 gtc ggc cct ggt gca ggt tct gat g Val y Pro y Ala Gy Ser Asp C 55 agc ccc gcg gaa ctc atc aaa act g Ser Pro Ala G u Leu Ile Lys Thr A 70 aaa gct tat gta gat tagtttctta tg Lys Ala Tyr Val Asp 1 i st cor r ect ed. t xt 635 l a Ala Lys Ile Ala 650 hr Leu Gy G n Pro 65 eu Lys Leu Ala Thr 685 sp G u Pro Thr Thr 700 sp Leu Phe Asp G n 715 3 u Hi s Hi s Leu G y 730 Sy Pro G y Ala G y '45 'ro Ala u Leu Ile 765 l a Tyr Val Asp 780 Lys Leu 670 Hi s Gy Leu Val Ser 750 Lys aaa Lys gt c Val at c SIle ttc Phe cgc Ar g Ar g 655 Thr Met Leu Val Leu 735 Asp Thr acc Thr at c SIe att SIe gag G u cac Hi s 640 Leu Thr Al a Hi s Asp 720 Al a Gy Asp ac ctc gct gat is Leu Ala Asp 10 iat gac ggc aag sp Asp G y Lys 25 ct cac gct gac la His Ala Asp gt ggc tcg at t 3y G y Ser lie at act cca aca sp Thr Pro Thr igaaaaccc tgg gt g Val tct Ser cac Hi s gt a Val gga G y <210> 148 <211> Page 165 seql i st cor r ect ed. t xt <212> PRT <213> Corynebact eri um gl ut am cum <400> 148 Leu Asp G u Pro Thr Thr G y Leu His Leu Ala Asp Va 1 5 10 Leu Asp Leu Phe Asp G n Leu Val Asp Asp y Lys Se 25 Ile u His His Leu y Val Leu Ala His Ala Asp Hi 40 4 Val y Pro y Ala y Ser Asp y y Ser Ile Va 55 Ser Pro Ala G u Leu I e Lys Thr Asp Thr Pro Thr G 70 75 Lys Ala Tyr Val Asp <210> 149 <211> 1663 <212> DNA <213> Cor ynebact er i um gl ut am cum <220> <221> CDS <222> (101)..(1663) <223> FRXA00834 <400> 149 tgttttagcc atggacccca tactagggag agttttgttt tggtgc caagcgcgaa caggcctatg caaacggtac gatatgacac atg ca I Lys Thr Leu r Val Ile Val s Ile Ile Asp I Phe u y y Arg His Leu taga aaaggttcac a aaa gct n Lys Al a t cc Ser aat Asn gt g Val gaa G u ttc Phe acc Thr tct Ser gat Asp tcg Ser gga Gy cgc Ar g ccg Pr o gcg Al a gt g Val at t I e cgc Ar g ggc Gy ttg Leu at g Met at c I e acc Thr tcg Ser at c SIle aag Lys at c SIle gca Al a 75 gt c Val gca Al a gt c Val cct Pr o t cc Ser aac Asn agg Ar g gat Asp act Thr cac Hi s aaa Lys tcg Ser 45 gaa G u ccc Pr o cag G n gat Asp ggt Gy agg Ar g ct g Leu acc Thr gat Asp gag G u gcc Al a 110 gcg Al a 15 cgt Ar g gt g Val t at Tyr gtt Val cag G n 95 acc Thr aat Asn ct c Leu ttc Phe agc Ser gac Asp at g Met gcg Al a Met 1 gaa G u acc Thr ggc Gy act Thr cat Hi s ggc Gy at g let G aac Asn gt g Val aca Thr ttt Phe ttg Leu gca Al a ttg Leu ct c Leu acg Thr gct Al a caa G n ggc G y cca Pr o 100 att SIe Page 166 ttt tcc cga Phe Ser Arg 120 atc gcg gaa Il e Ala G u seql i st cor r ect ed. t xt cct aac gcg ggt ggc ccg gg Pro Asn Ala G y Gy Pro G a gct tat tcc y Ala Tyr Ser n 125 13 ttc Phe ggc Gy 150 at g Met aaa Lys at c SIle tcg Ser gaa G u 230 ggc Gy at g let gt g Val cga Ar g gct Al a 310 acg Thr acc Thr cgc Ar g at c SIle gt c Val aac Asn cca Pr o ctt Leu ggt Gy 200 ctt Leu cac Hi s aac Asn tct Ser cga Ar g 280 gcg Al a ttg Leu gaa Gu gat Asp gct Al a 360 cat Hi s tct Ser aag Lys tgc Cys 170 gac Asp acc Thr gat Asp ttc Phe acc Thr 250 gat Asp gtt Val cat Hi s gcg Al a ccc Pr o 330 ttc Phe acg Thr ggc Gy gtt Val cgg Ar g 155 gag Gu gcc Al a cca Pr o gct Al a ctt Leu 235 t at Ty r cgc Ar g acc Thr gcc Al a at g bet 315 tcg Ser gt g Val tt g Leu tct Ser t cc Ser 140 gag Gu ggc Gy t cc Ser ggt Gy gcc Al a 220 t at Tyr gag G u gaa G u ttc Phe ttg Leu 300 gag Gu gtt Val gag Gu tct Ser gca Al a 380 gca Al a aaa Lys at g let ct c Leu gga Gy 205 aag Lys ctt Leu ggt Gy ggc Gy at t SIle 285 gag G u gt c Val gct Al a at c SIle ggt Gy 365 ttg Leu t cc Ser gct Al a ggc Gy t cc Ser 190 tgg Tr p ccg Pr o gag G u ctt Leu at g Met 270 cct Pr o t cc Ser cgt Ar g ccc Pr o ggt Gy 350 ggt Gy act Thr gcc Al a ttc Phe 160 gcc Al a aac Asn t at Tyr aag Lys acc Thr 240 ccc Pr o aag Lys cct Pr o at c SIle ttg Leu 320 ct c Leu ggc Gy gca Al a gt c Val at c l Ie 145 aaa Lys t ca Ser gac Asp cgg Ar g gat Asp 225 aag Lys cgc Ar g cat Hi s gcg Al a aat Asn 305 gcc Al a acc Thr t at Tyr cag G n acc Thr 385 acg Thr cgc Ar g gac Asp ggc Gy at g let 210 ttc Phe at g Met at t SIle at t SIle tgt Cys 290 ggc Gy aag Lys gca Al a at c SIle cgc Ar g 370 t at Tyr gaa G u ggt Gy gac Asp 180 ct g Leu t ca Ser gag Gu at c SIle aaa Lys 260 gcg Al a gga Gy aac Asn at c SIle act Thr 340 ct c Leu aag Lys ttt Phe aag Lys ggc Gy 165 ct c Leu acc Thr gaa Gu gaa Gu gct Al a 245 t cc Ser ttc Phe act Thr at c SIe aaa Lys 325 gaa Gu gat Asp at g Met gat Asp 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Page 167 seqI i st car r ect ed. t xt gaa ccc acc gcc ggt ttg cac gcc tac gac att gaa cgc atg aac aag Gu Pro Thr Ala G1y Leu His Ala Tyr Asp Ilie Gu Arg I'bt Asn Lys 390 395 t tg ct g ct c gat ct t cgc Leu Leu Leu As p Leu Ar g 410 cac aag ccg gaa acc at c Hi s Lys Pr o G31u Thr Ilie 425 cca ggt gca ggc gcg ggt Pro G31y Al a G31y Al a G31y 440 gac aag ctt aaa gac agc Asp Lys Leu Lys Asp Ser 455 cgg gcg tca t tg aag gaa Ar g Al a Ser Leu Lys G31u 470 475 atc cgc ggg gcc gat cga Ilie Ar g G y Al a Asp Ar g 490 ccg ct c ggc gt g t tc acg Pr o Leu G31y Val Phe Thr 505 t cc t cg t tg at t Ser Ser Leu Ilie 520 <210> 150 <211> 521 <212> PRT <213> Cor ynebact er i urn gi <400> 150 I'b t G3 n Lys Al a Asp Ser 1 5 a3 u Asn Asn Leu Lys Asn Thr Val Phe Thr G3 y Val1 G31y Thr I Ie Al a Al a G31u Thr Phe Val G31n G31y Ph e 70 Hi s Leu G3 u G3 y I I e Thr a31y Al a Asn Pr o Ar g Ser 1 00 I'b t Leu Ar g Ilie Leu Phe 115 Pro a31y Al a Tyr Ser Phe g at As p gcc Al a gga a31y g ac As p 460 t cc Ser aat As n gcg Al a aaa Ly s at t I Ie ggt a31y 445 ac c Th r gt g ValI aat As n at t I Ie ggc (31y gca Al a 430 g aa a31u gt g ValI cgt Ar g tt g Le u t cc Ser 510 400 ac c Th r cat Hi s cgg Ar g ggc a31y ccg Pr o 480 aat As n gt t ValI gt t ValI gt g ValI tt t Ph e ct c Le u 465 cat Hi s gt g ValI gca Al a ValI Pr o Ser As n Ar g As p Th r Pr o Ser tt a Le u gt g ValI gag a31u 450 cat Hi s ggc a31y g at As p ggt a31y Hi s Lys Ser a31u Pr a a31n As p As n 125 Al a gt c ValI g ac As p 435 ggg a31y tt t Ph e gcc Al a gt c ValI t cg Ser 515 a31y Ar g Leu Th r As p a31u Al a 110 Al a Ser gt g ValI 420 ct t Le u ag c Ser aat As n ct g Le u g at As p 500 ggt a31y Al a Ar g ValI Ty r ValI a31n Th r a31y a31y 1315 1363 1411 1459 1 507 1 555 1 603 1651 1 663 ut anm cumn Hi s Asp Tr p Val Ser Val 25 Ser a3 y Se r 40 Ser Ar g Ar g 55 I'b t Pr o Ser Thr Al a I I e Thr Val a31y 1 05 Ser Ar g I Ie 120 As n Val Pr o I I e Ser 10 Ar g I Ie a3 y Lys Leu I I e [vb t Al a 1 1 e Val 90 Thr Al a Al a a3 u Ser Val Page 1 6E seql i st cor r ect ed. t xt 135 G y Asn Thr 140 G u Lys Ala Thr SIle 145 Lys Ser Asp Ar g Asp 225 Lys Ar g Hi s Al a Asn 305 Al a Thr Tyr G n Thr 385 G u Val Val Phe Leu 465 Hi s Val Thr SIle Al a 195 Tyr Thr Lys G n Ar g 275 Gy Lys Tr p Leu G n 355 Thr Val l t Val Asp 435 Gy Phe Al a Lys Gy 165 Leu Thr G u G u Al a 245 Ser Phe Thr SIle Lys 325 G u Asp lbet Asp Lys 405 G u Gy Val Asp G u 485 Cys G u Pr o Gy 215 Ar g l Ie Leu Asp Leu 295 G u Val Leu Pr o Ar g 375 Pr o Leu Lys Gy Lys 455 Al a Ar g Pr o Leu Gy 200 Leu Hi s Asn Ser Ar g 280 Al a Leu G u Asp Al a 360 Hi s Thr Leu Pr o Al a 440 Leu Ser Gy Al a Phe 185 Tyr Phe Asn let Lys 265 Al a Pr o Cys Al a Asn 345 Gy Leu Al a Asp G u 425 Gy Lys Leu Al a Lys Arg 155 Cys u 170 Asp Al a Thr Pro Asp Al a Phe Leu 235 Thr Tyr 250 Asp Arg Val Thr Hi s Al a Al a lVet 315 Pro Ser 330 Phe Val Thr Leu G y Ser G y Leu 395 Leu Arg 410 Thr Ile Al a G y Asp Ser Lys G u 475 Gy Ser Gy Al a 220 Tyr G u G u Phe Leu 300 G u Val G u Ser Al a 380 Hi s Asp Al a Gy Asp 460 Ser Mbt Leu Gy 205 Lys Leu Gy Gy SIle 285 G u Val Al a SIle Gy 365 Leu Al a Lys l Ie Gy 445 Thr Val Ar g 175 Leu Ser SIle Pr o SIle 255 G n Cys Lys Asp Leu 335 Leu G u Asp Asp Asn 415 Asp SIle Thr Al a Phe 160 Al a Asn Tyr Lys Thr 240 Pr o Lys Pr o SIe Leu 320 Leu Gy Al a Val SIe 400 Thr Hi s Ar g Gy Pr o 480 Arg Asn Asn Leu Asn Asn 495 Page 169 seqi i st car r ect ed. t xt Val Asp Val Asp I Ie Pr o Leu G31y Val Phe Thr Ala I Ie Ser G31y Val 500 505 510 Ala G31y Se r G31y Lys Ser Ser Leu I Ie 515 520 <210O> 151 <21 1> 864 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (841) <223> RXA00995 <400> 151 gt cggat cat ct ggagggga acacccacac gcct t ct aga acgaagat gc t t tct tggct gccat tgacg gggt aaggt c agatacaggc aaaagct cat at g aac cct cac t at 11! l'bt Asn Pro His Tyr ct g Le u cgt Ar g tt t Ph e ac c Thr tt g Le u gag O31u t ac Tyr gt g ValI t gg Tr p ct t Le u 1 50 c aa O31n gcc A a at c I Ie t ac Tyr t cc Ser ac g Thr tt g Le u gt g ValI ac c Thr 120 t ct Ser ct g Le u ct g Le u gt c ValI ct g Le u t at Tyr gt c ValI gt g ValI ac c Thr tt c Ph e cgg Ar g ct c Le u ag c Ser tt g Le u 1 70 aaa Ly s at t I Ie t cc Ser at g vb t ac g Thr 75 ac g Thr ggc G31y t at Tyr at t I Ie t ct Ser 155 ct t Le u cga Ar g ct t Le u t cc Ser at c lIe at g vb t aac As n at c I Ie ct c Le u gct A a 140 gcg A a at t I Ie gt c ValI ct a Le u ac a Thr 45 gca A a c aa O31n at t I Ie at g vb t ct g Le u 125 gcg A a tt t Ph e tt g Le u ct g Le u gca A a ccg Pr a gt g ValI ag a Ar g c ac Hi s gcg A a 110 ggt O31y at c I Ie gcc A a ccc Pr a ct g Le u 15 ccc Pr a gca A a tt c Ph e g aa O31u cgc Ar g 95 gt g ValI gt g ValI ac c Thr ag c Ser c ag O31n 1 75 c ag G n gt g ValI ggc G3y ccc Pr a cgc Ar g 80 gt t ValI gcg A a g aa (3u ggt G3y act Thr 1 60 tt c Ph e 1 ct g Le u gcg A a ac c Thr tt g Le u 65 aac As n g at As p c aa O31n ac c Thr ct t Le u 145 gag O31u ct a Le u aaa Ly s tt g Le u c ag O31n 50 at g vb t gct A a tt g Le u t ct Ser g aa O31u 130 at c I Ie tt c Ph e tt g Le u gcc A a at g vb t 35 ct g Le u ct c Le u gga O31y at c I Ie tt g Le u 115 t cg Ser gga O31y c aa O31n t gc Cys g at As p 20 t cg Ser tt t Ph e at g vb t ac g Thr ggt O31y 1 00 ct c Le u gag O31u gt g ValI gct A a ggt O31y 1 aaa Ly s ct g Le u aag Ph e ct c Le u ggc O31y at g vb t t gg Tr p t ct Se r at c I Ie 1 tt g Le u Page 170 ct g Le u gt g ValI ac g Thr tt t Ph e 230 ac c Th r seqI i st car r ect ed. t xt cca cgg gat gat ct g ccg gat gt g t tg cgc tgg9 gt t t ct Pr oAr gAsp Asp Leu Pr oAsp Val Leu Arg Tr pVal Ser 185 190 195 ccg ct g t cc t at gca gt t gat gca gcg ct t gag gcc t ca Pr o Leu Ser Tyr Ala Val Asp Ala Ala Leu G31u Ala Ser 200 205 210 at cgga cag caa gt agt ggt caac at t gcc at c tgcgcc Ile (1y (1n (1n Val Val Val Asn IleAlalIleCys Aa 220 225 gt g agc t tc ct g ct g gt g gcg gcg ct a t cg at g ccg aga Val Ser Phe Leu Leu Val Ala Ala Leu Ser I'b t Pr o Ar g 235 240 t agat tact c t tccagcgag gt g <210O> 152 <21 1> 247 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 152 I'bt Asn Pro His Tyr Leu Leu A a Thr Le u A a Th r Le u As n As p O31n Th r Le u 145 O31u Le u Ar g Le u A a vb t Le u Le u G31y I Ie Le u 115 Ser G31y O31n Cys ValI 1 95 A a Lys5 Le u Lys5 Ph e Le u O31y vb t Tr p Ser I Ie 1 65 Le u As n Ar g Ser Ty r I Ie vb t Ar g O31y Le u I Ie 135 G31y Th r I Ie Le u (31y 215 A a vb t Th r Ser Tr p A a 1 05 Le u Th r Le u Pr a Ar g 1 85 Le u (31y Lys5 I Ie Ser vb t Th r 75 Th r O31y Ty r I Ie Ser 1 55 Le u As p Ty r Ar g Le u Ser lIe vb t As n I Ie Le u A a 140 A a I Ie Le u A a ValI Le u Th r A a O31n I Ie vb t Le u 125 A a Ph e Le u Pr a ValI 205 Le u A a Pr a ValI Ar g Hi s A a 110 G31y I Ie A a Pr a As p 1 As p Le u Pr a A a Ph e O31u Ar g ValI ValI Th r Ser O31n 1 ValI A a O31n ValI G31y Pr a Ar g ValI A a O31u G31y Th r 1 Ph e Le u A a On On Val Val Val Asn Ilie 220 Page 171 seqi i st car r ect ed. t xt Ala I Ie Cys Ala Ala Phe Ala Val Ser Phe Leu Leu Val Ala Ala Leu 225 230 235 240 Ser l'bt Pr o Ar g l'bt Thr Ar g 245 <210O> 153 <21 1> 1353 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1330) <223> RXN00803 <400> 153 t cat cct tcc t tagct cgcg t gagct tccc aagcgt aagc cggccgt tct gt taaagat t ggt ct ggcca t t tcct ccat acccccgt gt gagggcat aa at g ggg gt g t cc gcg 11! l'bt G31y Val Ser Ala ct t Le u t gg Tr p g aa G31u aac As n gt a ValI gcc A a at t I Ie ct g Le u at t I Ie gct A a 1 50 ggc (31y at g vb t gcg A a act Th r tt c Ph e tt g Le u at g vb t t cg Ser gga G31y 120 gag G31u gca A a gcg A a g ac As p gct A a tt g Le u g ac As p gca A a cct Pr o g aa G31u t cg Ser t ca Ser cgc Ar g gct A a 1 70 at g vb t t t a Le u ct g Le u ct t Le u gct A a 75 gcg A a gaa G31u gt g ValI t cg Ser gca A a 155 ggt (31y gt g ValI t cg Ser gt g ValI tt g Le u ggt G31y cca Pr o ac c Th r ggc G31y t cg Ser 140 t cg Ser gt g ValI gcg A a gt c ValI Ph e 45 ct g Le u cct Pr o Le u gcc A a at g vb t 125 cgg Ar g at g vb t at g aac As n tt t Ph e t ac Ty r gt g ValI tt a Le u at c I Ie at c I Ie 110 gt g ValI tt t Ph e t ct Ser gca A a aaa Ly s 15 ac g Th r cga Ar g tt t Ph e t ct Ser gcg A a 95 ct g Le u at g vb t gag G31u tt g Le u c ag (31n 1 75 cgg Ar g gt c ValI ggt G31y t at Ty r g ac As p at c I Ie at t I Ie ac a Th r cca Pr o ac c Th r 1 t gg Tr p 1 gca A a gcg A a g aa G31u gcc A a cgc Ar g tt g Le u gcc A a gcg A a ggg G31y 145 ggt G31y ct g Le u c ag (31n t gg Tr p ggg G31y at c I Ie t at Ty r ggt G31y at t I Ie gga G31y 130 gt g ValI ggt (31y cgt Ar g ggt G31y tt c Ph e gga G31y ggc G31y t cc Ser ggt G31y 115 ggt G31y aaa Ly s tt t Ph e aaa Ly s ggc G31y tt t Ph e gt a ValI cga Ar g gcg A a 1 00 cga Ar g t cc Ser ac c Th r gcg A a cca ct a cct gga Pro Leu Pro G1y 1 Page 172 seql i st cor r ect ed. t xt cag G n ttg Leu aag Lys cga Ar g 230 gcc Al a tct Ser tct Ser aaa Lys at c SIle 310 gtt Val ggg Gy ct g Leu gcc Al a ggc G y 390 agt Ser gca Al a at t SIle 200 t ca Ser ttg Leu act Thr ccc Pr o ttt Phe 280 cgc Ar g gcg Al a tgc Cys gcg Al a ggt Sy 360 at g Met ggt Sy cgc Ar g tat gtt ttg cac att att Tyr Val Leu His Ile Ile 185 19C aca gcg ccg gaa act cgt Thr Ala Pro G u Thr Arcg 205 ttc tgg tca gat gtg ctt Phe Trp Ser Asp Val LeL 220 ttt gtg gtt gct cca att Phe Val Val Ala Pro Ile 235 gcc tac gca gtt ttg ccg Ala Tyr Ala Val Leu Prc 250 gtt gcg tat tct gcg ctc Val Ala Tyr Ser Ala LeL 265 27C ggt atc caa caa ttc ggt Sy Ile n G n Phe G y 285 ggg ccg att ttg gcc atg Sy Pro lle Leu Ala IVet 300 gtg atc gtg gtg atg aac Val Ile Val Val Met Asr 315 atg gcc ctc ggc ctg tct IVet Al a Leu G y Leu Ser 330 gaa act caa aac att gct G u Thr G n Asn Ile Ala 345 35C att ttc tac tgc ctg acg Ile Phe Tyr Cys Leu Thr 365 acc tgg ttg aat caa tgg Thr Trp Leu Asn G n Tr p 380 gcg gtg atg gca act att Ala Val bet Ala Thr Ile 395 cga ttc tgagaaacaa ctaa Arg Phe 410 ctc act ctg att ttg Leu Thr Leu Ile Leu 195 caa tca gcg cac ctg G n Ser Ala His Leu 210 gtg cca tct gca cta Val Pro Ser Ala Leu 225 gga ccg tgg gtt ttc Gy Pro Trp Val Phe 240 tcg cag ctg cgt gac Ser G n Leu Arg Asp 255 atc gct ttg gtt acc Ile Ala Leu Val Thr 275 cct caa atc atg ggc Pro n lie IVet y 290 ttc gtc aca gtc atc Phe Val Thr Val lile 305 cct cat cca tgg tgg Pro His Pro Trp Trp 320 tat ggc ctg tgt atg Tyr G y Leu Cys IVet 335 cca cct att gat at g Pro Pro Ile Asp IVet 355 tac gta ggt atg gt c Tyr Val G y Met Val 370 ctc agt tac ccg ttc Leu Ser Tyr Pro Phe 385 tgt ctg atc att gtg Cys Leu Ile Ile Val ttc Phe aaa Lys gac Asp ggt G y at g Met 260 tta Leu acc Thr ggc G y gcg Al a ttc Phe 340 gca Al a ttt Phe at g Met agt Ser ccg Pr o act Thr aag Lys gcg Al a 245 gtt Val ggt G y tct Ser at g Met ct a Leu 325 at g Met ggc G y cca Pr o ct g Leu ttt Phe 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1353 tagtgag cca <210> 154 <211> 410 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 154 Page 173 seql i st cor NIet G y Val Ser Al a Leu Asn NIet Ser Asp 1 5 10 r ect ed. t xt lMt Val Ala Asn Lys Arg Al a Al a G u Al a Ar g Leu Al a Al a Gy 145 Gy Leu Leu Al a Ser 225 Tr p Leu Leu SIle Thr 305 Pr o Leu SIle Ar g Gy Phe Gy Gy Ser Gy 115 Gy Lys Phe Leu Leu 195 Leu Leu Phe Asp Thr 275 Gy SIle Tr p lbet Met 355 Val Asn Ser Al a Ar g Leu Val Tr p Ser Leu 165 Gy Pr o Thr Lys Al a 245 Val Gy Ser Met Leu 325 bet Gy Leu Phe Leu Gy Val Al a Ser Lys 135 Gy Pr o Leu Leu Gy 215 Phe Phe Al a Gy Thr 295 Gy Gy Leu Thr Al a Thr 40 Phe Leu let Ser Gy 120 G u Al a Al a Al a SIle 200 Ser Leu Thr Pr o Phe 280 Ar g Al a Cys Al a Gy 360 Val Pr o SIle Leu Leu Gy 105 I le Leu Lys Leu Tyr 185 Thr Phe Phe Al a Val 265 Gy Gy Val Met G u 345 I le Al a Leu Asp Al a Pr o 90 G u Ser Ser Ar g Al a 170 Val Al a Tr p Val Tyr 250 Al a SIle Pr o SIle Al a 330 Thr Leu Leu Leu Al a Al a G u Val Ser Al a 155 Gy Leu Pr o Ser Val 235 Al a Tyr G n SIle Val 315 Leu G n Val Phe Leu Pr o Leu Al a let 125 Ar g Met Met SIle Thr 205 Val Pr o Leu Al a Phe 285 Al a let Leu SIle Phe Tyr Val Leu SIle l Ie 110 Val Phe Ser Al a SIle 190 Ar g Leu SIle Pr o Leu 270 Gy Met Asn Ser Al a 350 Thr Ar g Phe Ser Al a Leu let G u Leu G n 175 Leu G n Val Sy Ser 255 I e Pr o Phe Pr o Tyr 335 Pr o Phe Tyr Cys Leu Thr Tyr Val 365 Page 174 seqI i st car r ect ed. t xt G31y I'bt Val Phe Pro Ala Leu I'bt Thr Tr p Leu Asn O31n Tr p Leu Ser 370 375 380 Tyr Pr o Phe [Vbt Leu G31y Phe G31y Ala Val [Vb t Ala Thr Ilie Cys Leu 385 390 395 400 Ilie Ilie Val Ser Phe Ser Ala Ar g Ar g Phe 405 410 <210O> 155 <21 1> 703 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (703) <223> FRXA00803 <400> 155 t cat cct tcc t tagct cgcg t gagct tccc aagcgt aagc cggccgt tct gt taaagat t ggt ct ggcca t t tcct ccat acccccgt gt gagggcat aa ct t Le u t gg Tr p g aa G31u aac As n gt a ValI gcc A a at t I Ie ct g Le u at t I Ie gct A a 1 50 at g vb t gcg A a act Thr tt c Ph e tt g Le u at g vb t t cg Ser gga G31y 120 gag G31u gca A a g ac As p gct A a tt g Le u g ac As p gca A a cct Pr o g aa G31u t cg Ser t ca Ser cgc Ar g at g vb t t t a Le u ct g Le u ct t Le u gct A a 75 gcg A a gaa G31u gt g ValI t cg Ser gca A a 155 gt g ValI t cg Ser gt g ValI tt g Le u ggt G31y cca Pr o ac c Thr ggc G31y t cg Ser 140 t cg Ser gcg A a gt c ValI tt t Ph e 45 ct g Le u cct Pr o tt g Le u gcc A a at g vb t 125 cgg Ar g at g vb t aac As n tt t Ph e t ac Tyr gt g ValI tt a Le u at c I Ie at c I Ie 110 gt g ValI tt t Ph e t ct Ser aaa Ly s 15 ac g Thr cga Ar g tt t Ph e t ct Ser gcg A a 95 ct g Le u at g vb t gag G31u tt g Le u cgg Ar g gt c ValI ggt G31y t at Tyr g ac As p 80 at c I Ie at t I Ie ac a Thr cca Pr o ac c Thr 1 60 at g vb t 1 gca A a gcg A a g aa G31u gcc A a cgc Ar g tt g Le u gcc A a gcg A a ggg G31y 145 ggt G31y c ag O31n t gg Tr p ggg G31y at c I Ie t at Tyr ggt G31y at t I Ie gga G31y 130 gt g ValI ggt G31y cgt Ar g ggt G31y tt c Ph e gga G31y ggc G31y t cc Ser ggt G31y 115 ggt G31y aaa Ly s tt t Ph e aaa Ly s ggc G31y tt t Ph e gt a ValI cga Ar g gcg A a 1 00 cga Ar g t cc Ser ac c Thr gcg A a ggg gt g t cc G y ValI Se r gcg A a gt c ValI aat As n ag c Ser gcg A a cgt Ar g tt g Le u gt g ValI t gg Tr p agt Se r ct c Le u 1 Page 175 INO seqI i st car r ect ed. t xt ggc cca gcg ct t gct ggt gt g at g gca cag tgg ctg cca caa cct gga 643 (3 Oy Pr o Al a Leu Al a O31y ValI I'bt Al a O31n Tr p Leu Pro O31n Pr o O31y 170 175 180 fl cag t tg gca t at gt t t tg cac at t at t ct c act ctg att ttg t tc ccg 691 31 On Leu Al a Tyr Val Leu Hi s I Ie Ilie Leu Thr Leu Ilie Leu Phe Pr o 185 190 195 t tg ct t at t aca 703 Leu Leu Il e Thr 200 S<210> 156 00 <211> 201 S<212> PRT S<213> Cor ynebact er i urn gi ut ani curn .C <400> 156 I~ ~t 1y Val Ser Ala LeuAsnIbt SerAspIbt Val Al aAsnLys Arg 1 5 10 Ala O31n Ar g Lys Val Trp Leu Ala Val Ala Leu Ser Val Phe Thr Val 25 Al a Tr p G y G y Asn G u Phe Thr Pr o Leu Leu Val Phe Tyr Ar g G y 40 O3 u 03 y Phe Phe Ser Asn Leu Phe Il e Asp Leu Leu Leu Val Phe Tyr 55 Al a IIe O31y Val Al a Val O31y Leu Leu Al a Al a O31y Pro Leu Ser Asp 70 75 Ar g Tyr G31y Ar g Ar g Al a Val I'b t Leu Pr o Al a Pr o Leu Ilie Al a Ilie 90 Leu 03 y Ser Al a Leu Il e Al a Ser 03 y 03 u 03 u Thr Al a Il e Leu Il e 100 105 110 Ala Ilie G31y Ar g Val Leu Ser G31y Ilie Ser Val G31y I'b t Val I'b t Thr 115 120 125 Al a 31y G31y Ser Tr p Ilie Lys O31u Leu Ser Ser Ser Ar g Phe O31u Pr o 130 135 140 G31y Val Lys Thr Ser Al a G31y Al a Lys Ar g Al a Ser I'b t Ser Leu Thr 145 150 155 160 G31y (1y Phe Al aLeu (1y Pr oAiaLeu Alia 1y Val I'b t Aia 1n Tr p 165 170 175 Leu Pr o O31n Pr o G31y O31n Leu Al a Tyr Val Leu His Ilie Ilie Leu Thr 180 185 190 Leu Ilie Leu Phe Pro Leu Leu Ilie Thr 195 200 <210> 157 <211> 1014 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221> CIDS <222> (991) <223> RXA01407 Page 176 seqI i st car r ect ed. t xt <400> 157 atccggggaa cggatcccaa agatctcctt gatgccatcg cgtttttaac ctggccagct ct ggt t gccc cagt gat cgc cccact t ct g ggaggt ct t c ttg caa gat acc att Leu On Asp Thr Ile ggt G31y at c SI e aag Lys gcg Al a ct t Leu ttt Phe at c SI e t ac Ty r caa O31n gca Al a 150 at t SI e ggc G31y ct g Leu ct C Leu cga Ar g gct Al a ttt Phe gcc Al a at c SI e gt g Val cgc Ar g ttg Leu 120 gcg Al a ttc Phe cgc Ar g ttg Leu ct C Leu 200 ttc Phe at c I e ct a Leu cgg Ar g at t SI e gca Al a t gg Tr p cca Pr o at c SI e ggg G31y ggc G31y aat Asn 170 ttg Leu gt g Val gcc Al a tt C Phe tt C Phe cca Pr o gcg Al a t gc Cys 75 ct g Leu ggt G31y acc Thr t gg Tr p aac Asn 155 tt C Phe gca Al a ct g Leu t ac Ty r ct C Leu at c SI e ggt G31y gag (31u ct C Leu cga Ar g ttc Phe gca Al a t ct Ser 140 gt g Val aaa Lys act Thr ct g Leu aac Asn 220 ct C Leu cag O31n ttc Phe 45 tta Leu gt c Val gt t Val cga Ar g gca Al a 125 gca Al a gca Al a cca Pr o ttt Phe ct C Leu 205 acc Thr aac Asn ccc Pr o ct C Leu at t SI e tta Leu cca Pr a gt g Val 110 cca Pr a aca Thr at c SI e gt a Val ttg Leu 190 ttt Phe ttg Leu gt g Val 15 aag Lys ggc G31y t gc Cys agt Ser ggc G31y 95 ggt G31y ttc Phe tta Leu aaa Lys ct g Leu 175 ttc Phe gt t Val cag O31n t ca Ser ccc Pr a aac Asn gca Al a agc Ser gct Al a 80 cga Ar g aat Asn at g Ivb t gcg Al a cct Pr a 160 gt c Val gt t Val t cg Ser ttt Phe gca Al a 240 1 tta Leu acg Thr at g Ivb t aga Ar g gcg Al a ct t Leu t cc Ser ttc Phe ggt G31y 145 ttc Phe ttt Phe cgt Ar g ggc G31y gt c Val 225 acc Thr gga G31y gcc Al a ct g Leu agt Ser gt g Val ttt Phe t cc Ser act Thr 130 gcc Al a acc Thr t ct Ser gca Al a gca Al a 210 gat Asp ct g Leu at c SI e gt g Val gt g Val ccg Pr a gt a Val gat Asp gga G31y 115 ttg Leu at g rvb t acg Thr aac Asn gat Asp 195 tta Leu at c SI e cac Hi s at c I e aat Asn at g Ivb t gcc Al a t gc Cys ct C Leu 100 agt Ser ct C Leu gt g Val ccg Pr a gct Al a 180 acc Thr agg Ar g t ca Ser caa O31n gcg Al a gt g Val gt g Val gca Al a ggt G y agc Ser at c SI e ttc Phe gt c Val at c SI e 165 gct Al a cca Pr a t cc Ser cca Pr a ct a Leu 245 gaa caa acc agc aac gcc aac gtg tta Ou n Thr Ser Asn Ala Asn Val Leu 230 235 Page 177 seqI i st car r ect ed. t xt ggc at g tct t t gggt at t gcg gt agca gt cat cgcc at g tcc ctt gca G31y I'b t Ser Leu G31y I Ie Al a Val Al a Val I Ie Al a I'b t Ser Leu Al a 250 255 260 ccc acc gcc aac t gg gca t tc cca ct g gca gca gcg t tg t tc ct c at t Pr o Thr Al a Asn Tr p Ala Phe Pr o Leu Al a Al a Al a Leu Phe Leu Ilie 265 270 275 cct cta atc ggc gca ct a tct ttg cct cgc gac ggc ggt gcc cga gcc Pr o Leu Ilie G31y Al a Leu Ser Leu Pr o Ar g Asp G31y G31y Al a Ar g Al a 280 285 290 t tt t cc t cc t ct t agaaaccca ct tct gaaag gt a Phe Ser Ser Ser 295 <210> 158 <211> 297 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 883 931 979 1014 <400> 158 Leu G3 n Asp Thr Le u Thr Ar g Al a Le u Ser Ph e G31y 145 Ph e Ph e Ar g G31y ValI 225 I Ie ValI ValI Pr o ValI As p G31y 115 Le u vb t Thr As n As p 1 95 Le u I Ie I e G3 y Cy s Al a I I e I'b t Val Lys Ar g Val Al a O31n Al a Leu Thr 70 G3 y Phe Val Ser I Ie I'b t I Ie Ty r Ar g Phe 03 n Val 135 Val Al a Leu 150 1I e I I e Lys 1 65 Al a 03 y Al a Pr o Leu Val Ser Leu G31y 215 Pr o 31u O31n 230 Ar g Al a Ph e 40 Al a I Ie ValI Ar g Le u 120 Al a Ph e Ar g Le u Le u 200 Ph e Thr Tr p G31y As p ValI Cys Ar g I Ie 1 ValI Ph e Al a Tr p ValI 1 li1e Ser Ser lIe 10 Le u Ar g I Ie Al a Tr p 90 Pr o I Ie G31y G31y As n 1 70 Le u ValI Al a Ph e Ph e Pr o Al a Cys 75 Le u G31y Thr Tr p As n 1 55 Ph e Al a Le u Tyr Le u O31n Ph e Le u ValI ValI Ar g Al a 125 Al a Al a Pr a Ph e Le u 205 Thr ValI Ly s G31y Cys Ser G31y G31y Ph e Le u Ly s Le u 1 Ph e ValI O31n Pr a As n Al a Ser Al a Ar g As n vb t Al a Pr a 1 ValI ValI Se r Ph e Asn Al a Asn Val Leu Ser Al a 235 240 Page 178 seql i st cor r ect ed. t xt Thr Leu Hi s G n Leu G y Met Ser Leu G y I e Ala Val Ala Val I e 245 250 255 Ala IVet Ser Leu Ala Pro Thr Ala Asn Trp Ala Phe Pro Leu Ala Ala 260 265 270 Ala Leu Phe Leu lie Pro Leu lie y Ala Leu Ser Leu Pro Arg Asp 275 280 285 Gy y Ala Arg Ala Phe Ser Ser Ser 290 295 <210> 159 <211> 324 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(301) <223> RXA01408 <400> 159 cgcatagagt tatctcgaaa ctaccaagac ggtttctttt ctttagatct aaggagga gaccgtcttt caccctttca tctgattgga catcgacgcc atg cgc aat gat cgg ga tcc ttt agc gtt ccc att g Ser Phe Ser Val Pro II e Al gaa atc ctc gac ggc acc at Gu Ile Leu Asp y Thr II cgt gac ttc ggt att gac g Arg Asp Phe Gy Ile Asp Al tac ttg gca gcc gca gca g Tyr Leu Ala Ala Ala Al a Al tagcggatcg atttggtgtg cgc <210> 160 <211> 67 <212> PRT <213> Corynebacteri um gl ut <400> 160 IVet Arg Asn Asp Arg Ser P 1 5 G y Al a Leu Phe Leu G u Il Val Pro Ala lle Ala Arg A lie Ala Leu Val Ala Tyr L Gn Gy Gy cg a :c e cc a ct a 60 ct a Leu ct g Leu gt g Val 45 ggc Gy ctt Leu aca Thr 30 gat Asp at t SIle gcc Al a 15 acc Thr gt c Val ccg Pr o gcg Al a gca Al a agc Ser ct g Leu Iet Arg Asn Asp Arg 1 gga gca ctg ttt cta G y Al a Leu Phe Leu gtg cca gct att gct Val Pro Ala lie Ala att gca ctg gtt gct Ile Ala Leu Val Ala cag ggt ggc Gn Gy y arm cum he Ser Val e Leu Asp 25 sp Phe G y 40 eu Al a Al a 55 Pro lie Ala Leu Leu Ala Ala 10 Sy Thr lie Leu Thr Thr Ala Ile Asp Ala Val Asp Val Ser Alaa Aa Ala Gy Ile Pro Leu Page 179 seqi i st car r ect ed. t xt <210> 161 <211> 1275 <212> DNA <213> Cor ynebact er i ur gl ut ani cum <220> <221> CDS <222> (101)..(1252) <223> RXN01922 <400> 161 acactccttt ggtcacctgg tttggttgag ggaaacagac cccaagaaaa catgctgctt atgaattaaa gtgagcaccc cgcccaagaa cccaagaaat atg aga tca gga aac 11! l'b t Arg Ser 3y Asn gcc Al a t gg Tr p tta Leu ct g Leu ttt Phe gga G31y gga G31y acc Thr ggc G31y ggg G31y 150 gt t Val gcc Al a cgc Ar g gcc Al a gt a Val ct t Leu gcc Al a ct t Leu ttc Phe t gg Tr p 120 at t SI e ggg G31y ct C Leu agc Ser ttc Phe cat Hi s agc Ser cca Pr o at g lvb t ct t Leu gt t Val ggc G31y acc Thr gcg Al a ct g Leu 170 ccg Pr o at a SI e tt c Phe gt g Val agt Ser gt g Val 75 tt a Leu ggt G31y aga Ar g gcc Al a t cg Ser 155 at c SI e agc Ser ggt G31y gcg Al a ct g Leu ttg Leu gt a Val gcg Al a ct g Leu ttg Leu ggt G31y 140 aca Thr gcg Al a gca Al a gt t Val t ca Ser gt c Val 45 ct c Leu ct c Leu ttt Phe ggc G31y cgc Ar g 125 ttc Phe agc Ser gt g Val ct t Leu acc Thr gt g Val aac Asn gca Al a acc Thr cat Hi s gt t Val 110 gga G31y at g lvb t at t SI e gt t Val ggc G1y 1 90 at c I e 15 ttg Leu ggc G31y ggc G31y gga G31y gat Asp 95 gga G31y gca Al a at g lvb t at t SI e gt g Val 175 gca Al a ct g Leu gt g Val gct Al a ggt G31y ggt G31y 80 ggt G31y tta Leu agc Ser ggg G31y ac g Thr 160 gga G31y t cc Ser 1 ct g Leu ttg Leu ttt Phe gt g Val gt a Val cct Pr o gt c Val ggc G31y ccg Pr o 145 ccc Pr a ttt Phe agc Ser ttt Phe at c SI e ggt G31y ct t Leu ct t Leu t cc Ser gt c Val gt g Val 130 at t SI e ttt Phe gcg Al a gga G31y act Thr cgt Ar g 35 at t SI e gcc Al a t ct Ser ct c Leu agc Ser 115 aca Thr gt g Val gcc Al a ct t Leu at c SI e 195 gca Al a 20 gaa G u t at Tyr gac Asp gcg Al a ct g Leu 100 gcg Al a ttg Leu aca Thr at a SI e ggc G3y 180 aaa Lys gga G31y caa (31n gca Al a cgt Ar g ct t Leu gt a Val ggc G31y gcc Al a agt Ser agc Ser 165 gat Asp cac Hi s Page 180 s gaa cga agc at g aaa aag gcc ct C (31u Ar g Ser I'bt Lys Lys Al a Leu 200 205 eqI i st car r ect ed. t xt gcg gt g t cc t tg cc Al a Val Ser Leu Pr g at g gca at t a I'bt Al a IlIe t gg Tr p at c I Ie 230 gcg Al a tt c Ph e gcg Al a ct t Le u ct g Le u 310 cgt Ar g ggg G31y at t I Ie tt c Ph e t cc Ser gcc Al a t gg Tr p tt t Ph e 280 gt t ValI g aa O31u ac c Thr cca Pr o ct g Le u 360 t gc cys tt c Ph e ag c Ser 250 cgt Ar g ct g Le u gcc Al a ct c Le u at c I Ie 330 ct t Le u gcg Al a at c G31y 235 gca Al a ggc G31y gca Al a t cg Ser ct c Le u 315 ggc G31y ct c Le u ct g Le u ac c Thr 220 aac As n ggc G31y t cc Ser gct Al a at c I Ie 300 ag c Ser at c I Ie g ac As p gcg Al a ac c Thr gcc Al a ct g Le u gga G31y tt t Ph e 285 ct g Le u at c I Ie t at Tyr gcc Al a gcg Al a 365 t cc Ser at t I Ie at c I Ie at c I Ie 270 ggt G31y tt c Ph e g aa O31u t at Tyr ct c Le u 350 ct c Le u ct g Le u ct t Le u gca Al a 255 gt g ValI G31y ggc G y act Thr gt g ValI 335 ct c Le u gcc Al a at c I Ie ct c Le u 240 c aa O31n G31y g ac As p ac c Thr t ac Tyr 320 tt c Ph e ccg Pr o ct t Le u gt g ValI 225 ccc Pr o tt t Ph e gcg Al a t cc Ser gca Al a 305 ac g Thr ac g Thr c ac Hi s ggc (31y 210 at g t cc N/t Ser gga at c G31y I Ie t ta ggt Leu 03 y ct g t gt Leu Cys 275 at t cca I Ie Pr o 290 t at ggc Ty r G y cca ct c Pro Leu t at tt g Tyr Leu ctt ggc Leu 03 y 355 t cc gca Ser Al a 370 gcg Al a ggc G31y ag g Ar g 260 gcc Al a gt g ValI ct c Le u aac As n gga G31y 340 gcc Al a gt a ValI 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 cgc ggc gt a caa at c aag cgc ggg t at gt g gt t t agat t tct a cct acgacct 1272 Arg Gy Val On Ilie Lys Arg Gy Tyr Val Val 375 380 gaa 1275 <210O> 162 <21 1> 384 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 162 I'b t Ar g Se r G31y As n Al a As n Ar g ValI Ph e I Ie G31y ValI Th r I Ie Le u 1 5 10 Leu Phe Thr Al a G31y Trp Ala Ala Asn His Phe Al a Ser Val Leu Val 25 Leu I Ie Ar g 31u O31n Leu Asp Val Ser Ser Val Leu Val Asn G31y Al a 40 Phe (3y I Ie Tyr Al a Leu (3y Leu Leu Pr o Ser Leu Leu Al a 31y G31y 55 Page 181 seqi i st car r ect ed. t xt ValI ValI Pr o ValI G31y Pr o 145 Pr o Ph e Ser Le u ValI 225 Pr o Ph e A a Ser A a 305 Thr Thr Hi s (31y L eu Ala As p Ar g Ph e G31y Ala Ar g l'b t Val Val Leu Thr 03 y Ser Le u Ser 115 Thr ValI A a Le u I Ie 1 95 vb t Ser I Ie G31y cys 275 Pr a G31y Le u Le u G31y 355 A a Le u ValI G31y A a Ser Ser 1 65 As p Hi s I Ie Ar g A a 245 Ly s Le u Tr p cys Ar g 325 Ph e Ser I Ie G31y G31y Thr G31y G31y 150 ValI A a (31u Tr p I Ie 230 A a Ph e A a Le u Le u 310 Ar g G31y I Ie Ar g As n Ar g A a I Ie 135 Le u A a Ar g Ar g ValI 215 As p I Ie A a G31y Ph e 295 Ar g G31y Le u Pr a G31y 375 Ser I Ie 1 05 A a Le u vb t Ser Thr 1 85 vb t Ser Thr Ph e G31y 265 A a I Ie G31y G31y ValI 345 Tyr (31n Le u 90 ValI G31y Thr A a Le u 1 70 Pr a Ly s cys Ph e Ser 250 Ar g Le u A a Le u I Ie 330 Le u A a I Ie Le u G31y Ar g A a Ser 1 li1e Ser Ly s I Ie G31y 235 A a G31y A a Ser Le u 315 G31y Le u Le u Ly s A a Le u Le u G31y 140 Thr A a A a A a Thr 220 As n G31y Ser A a I Ie 300 Ser I Ie As p A a Ar g 380 Ph e G31y Ar g 125 Ph e Ser ValI Le u Le u 205 Thr A a Le u G31y Ph e 285 Le u I Ie Tyr A a A a 365 G31y Hi s ValI 110 G31y vb t I Ie ValI G31y 1 A a Ser le le le 270 G31y Ph e G31u Tyr Le u 350 Le u Tyr As p G31y A a vb t I Ie ValI 1 A a ValI Le u Le u A a 255 ValI G31y G31y Thr ValI 335 Le u A a ValI <210O> 163 <211> 1130 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> Page 182 seql i st cor r ect ed. t xt <221> CDS <222> (1)..(1107) <223> FRXA01922 <400> 163 ctg ctg ttt Leu Leu Phe 1 gtg ttg atc Val Leu Ile gct ttt ggt Al a Phe G y ggt gtg ctt G y Val Leu ggt gta ct t G y Val Leu ggt cct t cc G y Pro Ser tta gtc gt c Leu Val Val agc ggc gtg Ser G y Val 115 ggg ccg att Sy Pro lle 130 acg ccc ttt Thr Pro Phe 145 gga ttt gcg G y Phe Al a t cc agc gga Ser Ser G y tcc ttg ccg Ser Leu Pro 195 atc gtg at g Ile Val 'bet 210 ctc ccc gga Leu Pro G y 225 caa ttt tta G n Phe Leu gca Al a 5 gaa G u t at Tyr gac Asp gcg Al a ct g Leu gcg Al a ttg Leu aca Thr at a SIle ggc G y 165 aaa Lys gca Al a gcg Al a ggc G y agg Ar g 245 gga tgg gca gcc aat cat ttc gcg tca gtg ttg Sy Trp Ala Ala Asn His Phe Ala Ser Val Leu 10 caa G n gca Al a cgt Ar g ctt Leu 70 gt a Val ggc G y gcc Al a agt Ser agc Ser 150 gat Asp cac Hi s at t l Ie cgc Ar g gcg Al a 230 aaa Lys tta Leu ct g Leu ttt Phe 55 gga G y gga G y acc Thr ggc G y ggg G y 135 gtt Val gcc Al a gaa G u tgg Tr p at c SIle 215 gcg Al a ttc Phe gac Asp gga G y 40 ggt G y aac Asn cga Ar g gca Al a at t SIle 120 ttg Leu gcc Al a cgc Ar g cga Ar g gt g Val 200 gac Asp at c l Ie gcg Al a gt a Val ctt Leu gcc Al a ctt Leu ttc Phe tgg Tr p 105 at t SIle ggg G y ct c Leu agc Ser agc Ser 185 ttc Phe t cc Ser gcc Al a tgg Tr p t ca Ser ctt Leu cgc Ar g tct Ser at c I e 90 gcg Al a ct g Leu at g Met tcg Ser acc Thr 170 at g Met agc Ser acc Thr ttc Phe ggt Gy 250 agc Ser cca Pr o at g Met ctt Leu 75 gtt Val ggc G y acc Thr gcg Al a ct g Leu 155 ccg Pr o aaa Lys tgc Cys ttc Phe agc Ser 235 cgt Ar g gt g Val agt Ser gt g Val tta Leu ggt G y aga Ar g gcc Al a tcg Ser 140 at c SIle agc Ser aag Lys at c SIle ggc G y 220 gca Al a ggc Gy ct g Leu ttg Leu gt a Val gcg Al a ct g Leu ttg Leu ggt G y 125 aca Thr gcg Al a gca Al a gcc Al a acc Thr 205 aac Asn ggc G y t cc Ser gt c Val ct c Leu ct c Leu ttt Phe ggc G y cgc Ar g 110 ttc Phe agc Ser gt g Val ctt Leu ct c Leu 190 acc Thr gcc Al a ct g Leu gga Gy aac Asn gca Al a acc Thr cat Hi s gtt Val gga G y at g Met at t SIle gtt Val ggc G y 175 gcg Al a t cc Ser at t l Ie at c SIle at c SIle 255 ggc G y ggc Sy gga G y gat Asp gga G y gca Al a at g Met att SIe gtg Val 160 gca Al a gtg Val ctg Leu ctt Leu gca Al a 240 gtg Val Page 183 IND seqI i st car r ect ed. t xt ggc gcg ct g t gt gcc ctc gcg ggt t tt gcg ct g gca gct t tt ggt ggc 816 3 Gy Aa Leu Cys Aa Leu Aa (1y Phe AaLeu Aa AaPhe (1y (1y 260 265 270 c.gac tcc att cca gt g tgg ctt t t cgt t at cgcc tcg at cct gttc ggc 864 SAsp Ser Ilie Pr o Val Tr p Leu Phe Val Ilie Al a Ser Ilie Leu Phe G31y 275 280 285 acc gca t at ggc ct c t gc ct g cgc gaa ggc ct c ct c agc at c gaa act 912 Thr Al a Tyr G31y Leu Cys Leu Ar g 31u G31y Leu Leu Ser Ilie (31u Thr 290 295 300 t tac acg cca ct c aac cga cgt ggc acc ggc at c ggc at c t at t at gt g 960 00 Tyr Thr Pr o Leu Asn Ar g Ar g G31y Thr 31y Ilie 31y Ilie Tyr Tyr Val S305 310 315 320 tK tc acg t at t tg gga t tc ggg ct g cca gt g ct t ct c gac gcc ct c ct c 1008 .C Phe Thr Tyr Leu G31y Phe G31y Leu Pro Val Leu Leu Asp Ala Leu Leu 325 330 335 C1 ccg cac ctt ggc gcc tcc att ccg ctg tac gcg ctg gcg gcg ctc gcc 1056 Pr o Hi s Leu G31y Al a Ser Ilie Pr o Leu Tyr Al a Leu Al a Al a Leu Al a 340 345 350 ct t ggc t cc gca gt a at c cgc ggc gt a caa at c aag cgc ggg t at gt g 1104 Leu G31y Ser Al a Val Ilie Ar g G31y Val O31n Ilie Lys Ar g G31y Tyr Val 355 360 365 gt t t agat t tct a cct acgacct gaa 1130 ValI <210> 164 <211> 369 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 164 Leu Leu Phe Thr Ala G31y Tr p Ala Al a Asn Hi s Phe Al a Ser Val Leu 1 5 10 Val Leu I Ie Ar g 31u O31n Leu Asp Val Ser Ser Val Leu Val As n G31y 25 Al a Phe G31y I Ie Tyr Ala Leu G31y Leu Leu Pr o Ser Leu Leu Al a G31y 40 G31y Val Leu Al a Asp Ar g Ph e G31y Al a Ar g I'b t Val Val Leu Thr G31y 55 G31y Val Leu Ser Al a Leu G31y Asn Leu Ser Leu Leu Al a Phe Hi s Asp 70 75 G31y Pr o Ser Leu Leu Val G31y Ar g Phe Ilie Val G31y Leu G31y Val G31y 90 Leu Val Val Ser Al a G31y Thr Al a Tr p Al a G31y Ar g Leu Ar g G31y Al a 100 105 110 Ser G31y Val Thr Leu Al a 31y Ilie Ilie Leu Thr Al a G31y Phe I'b t I'b t 115 120 125 G31y Pr o Ilie Val Thr Ser G31y Leu G31y N/b t Al a Ser Thr Ser Ilie Ilie 130 135 140 Thr Pr o Phe Al a Ilie Ser Val Al a Leu Ser Leu Ilie Al a Val Val Val Page 184 seql i st cor r ect ed. t xt 145 Gy Ser Ser SIle Leu 225 G n Gy Asp Thr Tyr 305 Phe Pr o Leu Val Al a Gy Pr o 195 bet Gy Leu Leu SIle 275 Tyr Pr o Tyr Leu Ser 355 Gy 165 Lys Al a Al a Gy Ar g 245 Al a Val Leu Asn Gy 325 Al a Val Asp Ala Arg Ser Hi s l Ie Ar g Al a 230 Lys Leu Tr p Cys Ar g 310 Phe Ser SIle u Tr p SIle 215 Al a Phe Al a Leu Leu 295 Ar g Gy I e Ar g Ser 185 Phe Ser Al a Tr p Phe 265 Val G u Thr Pr o Leu 345 Val 155 Thr Pro Ser Ala Leu Gy 170 175 Met Lys Lys Ala Leu Ala 190 Ser Cys Ile Thr Thr Ser 205 Thr Phe Gy Asn Ala lie 220 Phe Ser Al a G y Leu Il e 235 Sy Arg y Ser Gy lie 250 255 Al a Leu Al a Al a Phe G y 270 lie Ala Ser Ile Leu Phe 285 Sy Leu Leu Ser Ile G u 300 G y i e G y i e Tyr Tyr 315 Val Leu Leu Asp Ala Leu 330 335 Tyr Ala Leu Ala Ala Leu 350 Sn Ile Lys Arg y Tyr 365 160 Al a Val Leu Leu Al a 240 Val Gy Gy Thr Val 320 Leu Al a Val <210> 165 <211> 362 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> RXA02060 <400> 165 gaa ttt gcc cgc att ttg aag cca aag gga cag gtc atc gtg ctt acc G u Phe Ala Arg lie Leu Lys Pro Lys Gy G n Val Ile Val Leu Thr 1 5 10 gca gat acc ggc cac ttg gct gag ctt cgt gaa cca ctg ggc atc att Al a Asp Thr G y His Leu Al a G u Leu Arg G u Pro Leu G y lie lie 25 gat gtg gag gcc ggc aaa gtt gat cgc atg atc gaa caa gcg gca ggc Asp Val u Al a Gy Lys Val Asp Arg IVet I Ie G u n Ala Al a y 40 Page 185 cac Hi s ct g Leu cgc Ar g gaa G u at c SIe seql i st cor r ect ed. t xt ctc aag cca gtt ggc gaa aga gac ttg gtg gaa ttt Leu Lys Pro Val G y G u Arg Asp Leu Val G u Phe 55 gat caa aaa tcc att gca tct cag atc ggt atg agc Asp n Lys Ser Ile Ala Ser n Ile Gy ebt Ser 70 75 cac att aag cct gag gct ttg gcg gaa cgc atc gcc His lie Lys Pro G u Ala Leu Ala G u Arg lie Ala 90 caa atg aag gtt aca gcc cgg gcc aag atc acc agg G n IVet Lys Val Thr Ala Arg Ala Lys Ile Thr Arg 100 105 taactcttat ctcactgggc ctt gaa G u cct Pr o gct Al a ct g Leu 110 at g ct g IVet Leu tct gca Ser Al a ct a cca Leu Pro gaa cgc G u Arg <210> 166 <211> 113 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 166 Su Phe Ala Arg lie Leu Lys Pro Lys 1 5 Al a Asp Thr G y Hi s Leu Al a G u Leu 25 Asp Val G u Ala y Lys Val Asp Arg 40 His Leu Lys Pro Val G y G u Arg Asp Leu Asp G n Lys Ser I e Ala Ser G n 70 Arg His lie Lys Pro G u Ala Leu Ala G u G n Met Lys Val Thr Ala Arg Ala 100 105 I e G n Su I e Val Sy Ar g l Ie SIle Leu G n Phe Ser Al a Ar g Val Sy Al a G u Pr o Al a Leu 110 Leu I e Al a bet Ser Leu G u <210> 167 <211> 1395 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1372) <223> RXN01936 <400> 167 gcgcggtgac accacagccg ttgtcagcgg cgcttggtct gtggaggatc gccgaggtta ctaacaaata ggcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115 Val Ser Phe Arg Asp 1 Page 186 seql i st cor r ect ed. t xt at t SIle tgg Tr p gcc Al a ggc G y t at Tyr tgc Cys acc Thr tta Leu ctt Leu aat Asn 150 ggt Gy gtt Val at c SIle gat Asp gt g Val 230 ccc Pr o at g M\et gct Al a ggc G y ccg Pr o acc Thr gga G y acg Thr tta Leu cag G n 120 agt Ser ct g Leu ttg Leu t cc Ser cca Pr o 200 ct g Leu gat Asp at c SIle gcg Al a acc aga ccg ctg aaa gaa ccg gcc Thr Arg Pro Leu Lys G u Pro Ala 15 gtt gcc acc gtc att ggt gcc caa Val Ala Thr Val Ile Gy Ala n cag att tac caa atg act ggg tcc G n Ile Tyr G n IVet Thr G y Ser 45 ctt ttt ggc ctt att cct ttg gtt Leu Phe Gy Leu lie Pro Leu Val att gcg gat gct ttt gat aaa cgc lie Ala Asp Ala Phe Asp Lys Arg 75 ggc at g tgt gtc acc act gcc ggt G y bet Cys Val Thr Thr Al a G y 95 aat gag aat att tgg ctc ctg tta Asn G u Asn lie Trp Leu Leu Leu 110 ttt ttc gcg gtg aat caa ccc acc Phe Phe Ala Val Asn G n Pro Thr 125 ttg ccg att gat caa tta gcg tcg Leu Pro lie Asp G n Leu Ala Ser 140 145 atg cag acc ggc gca atc gtt ggc Met n Thr Gy Ala Ile Val y 155 160 ccg ctg atc ggt ttc ggg tgg ctg Pro Leu Ile y Phe y Trp Leu 170 175 atc ccc aca ctg tgg gct gta tgg lie Pro Thr Leu Trp Ala Val Trp 190 ggc aag gtg atg aag gct ggt ttc G y Lys Val IVet Lys Ala G y Phe 205 tat ttg gct ggc caa ccc gtg ttg Tyr Leu Ala Gy G n Pro Val Leu 220 225 atc gcc atg att ttc ggc atg cca Ile Ala IVet Ile Phe y IVet Pro 235 240 gaa gtg aac ttc ggt ggg ggt gac Su Val Asn Phe G y y y Asp 250 255 atg tac tca tcc atg gct gtt ggc Iet Tyr Ser Ser Iet Ala Val 3 y 270 Page 187 ttc aaa cgc ctc Phe Lys tta act Leu Thr t cc ggc Ser G y att ttt Ile Phe atc gt g Ile Val ttt tgg Phe Trp at a aac Ile Asn 115 cga acg Arg Thr 130 gca aca Al a Thr ccg ctg Pro Leu tat ttc Tyr Phe t ca ct g Ser Leu 195 gcc agt Al a Ser 210 ttg atg Leu IVet cgt gcg Arg Ala gcc ggt Al a G y gca gtt Al a Val 275 Ar g gtt Val t at Tyr ggc G y ctg Leu gtg Val 100 ttt Phe gcg Al a t ca Ser at c SIe ctt Leu 180 cct Pr o gtg Val gtg Val ctt Leu gca Al a 260 ctt Leu seql i st cor r ect ed. t xt ggc Gy gtt Val gt g Val 310 ttc Phe gca Al a ggc G y tta Leu tgg Tr p 390 tgt Cys acc Thr ctg tct ggt Leu Ser G y 280 tgg tgc atc Trp Cys Ile att gtt gtc Ile Val Val at c at g at g Ilie e t bet 330 cga aac gct Arg Asn Ala 345 at c caa ggt lie On y 360 gac gtc ctt Asp Val Leu gta tta tgg Val Leu Trp gtg gcg gt g Val Al a Val 410 at c t aaat ac Ile gt g Val gcc Al a 300 ccc Pr o at t SI e ttg Leu tgg Tr p ggt Gy 380 gga Gay aaa gcc Al a 285 tgg Tr p ggc G y ggt G y cag G n at c SIle 365 tgg Tr p gt a Val ttc cgg att agc cgc cag ggt gtt gca Arg Ile Ser Arg n Gy Val Ala 290 ggc gca gcc gtt gct ttg ggt ggt Gy Ala Ala Val Ala Leu Gy y 305 gcg gtg act gcg tgg gcg tgg atg Ala Val Thr Ala Trp Ala Trp bet 320 325 ggc atg gct gac atg ttc agc tcg Gy IVet Ala Asp IVet Phe Ser Ser 335 340 cag tct gct gcg gaa cat gtg cag Sn Ser Ala Ala G u His Val n 350 355 atc gtc gtg gtg ggt gga cct cgt lie Val Val Val Gy Gy Pro Arg 370 gcc gct gag ccc ctc ggc gca ggt Ala Ala G u Pro Leu G y Ala G y 385 gcg gtg gtt gta ctc act gca att Ala Val Val Val Leu Thr Ala lle 400 405 tgg aaa tac gag aaa cca aaa att Trp Lys Tyr G u Lys Pro Lys Ile 415 420 979 1027 1075 1123 1171 1219 1267 1315 1363 1395 Pro Lys Phe t a tccatgccca ttt <210> 168 <211> 424 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 168 Val Ser Phe Arg Asp Ile Phe Ala Asp 1 5 Ala Phe Lys Arg Leu Trp Leu y Asn G n Leu Thr Val Val Al a Val Pro Val 40 Ser Ser y Tyr Val G y Leu Thr y Val lie Phe y Leu Tyr Gy y Ser 70 Arg li e Val Leu Ile Cys Thr Thr Ile G y Phe Trp Val Leu Thr Ile Leu G y 100 105 Thr Arg I 10 Val Ala Gn lie Leu Phe I e Ala 75 Sy lMt 90 Asn Gu Page 188 Leu Val G n Leu Al a Val SIle G u Sy Thr Pr o Asp Thr Leu Pr o Al a Sy Leu Lys Al a Leu seqi i st car r ect ed. t xt Leu lieAsn Phe Ser Leu On On Ala Phe Phe Ala Val Asn On Pro 115 120 125 Thr Ser 145 0Oy Le u Tr p Ph e Le u 225 Pr o As p 0Gy Ar g ValI 305 A a As p A a ValI Pr o 385 ValI 0Ou Thr Thr Le u Ph e Le u 1 95 Ser vb t A a 0Gy ValI 275 0Gy Le u A a Ph e Hi s 355 0Gy 0Gy Thr Pr o I Ie Le u A a 1 65 As p Ser ValI vb t Tyr 245 Thr 0Gy A a 0Gy vb t 325 Ser O n Ar g 0Oy I Ie 405 li1e Ar g 135 vb t A a ValI Ly s 0Oy 215 Le u G u Le u ValI Tyr 295 A a I Ie ValI Ar g A a 375 Thr vb t 0 y I Ie Le u I Ie I Ie 1 85 Ser Ly s Le u A a Ph e 265 Ser cys ValI vb t As n 345 O n ValI Le u A a Pr o O n 1 Le u Pr a Ly s Le u A a 235 ValI Tyr Tr p I Ie Ser 315 ValI I Ie ValI Hi s 0Oy 395 Pr a I Ie 140 Thr I Ie Thr ValI A a 220 vb t As n Ser ValI A a 300 Pr a I Ie Le u Tr p 0Oy 380 0Oy Ly s As p 0Oy 0Oy Le u vb t 205 0Oy I Ie Ph e Ser A a 285 Tr p 0Oy 0Oy O n I Ie 365 Tr p ValI Ph e O n A a Ph e Tr p 1 Ly s O n Ph e G y vb t 270 Ar g 0Oy A a 0Oy O n 350 li1e A a A a Tr p Le u I Ie 0Oy 1 A a A a Pr a 0Oy G y 255 A a I Ie A a ValI vb t 335 Ser ValI A a ValI Ly s 415 <21 0> 169 <21 1> 945 <212> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> Page 189 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(922) <223> FRXA01936 <400> 169 tttacagcag gcatttttcg cggtgaatca acccacccga tttgccgatt gatcaataag cgtcggcaac atcactgaat acc Thr at c SIle aca Thr gt g Val gct Al a at g Met aac Asn t ca Ser gt g Val gcc Al a 150 ccc Pr o at t SIle ttg Leu tgg Tr p gca Al a ttc Phe tgg Tr p aag Lys caa G n ttc Phe ggt Sy at g Met 120 cgg Ar g ggc Gy gcg Al a ggc Gy cag G n 200 at c SIle gtt Val tgg Tr p gt a Val ggt Gy gt g Val at g Met ggt Sy gtt Val agc Ser gcc Al a act Thr 170 gct Al a gct Al a gt g Val ggc Gy ct g Leu tgg Tr p tt c Phe tt g Leu 75 cca Pr o gac Asp ggc G y cgc Ar g gtt Val 155 gcg Al a gac Asp gcg Al a gt g Val ccg Pr o t at Tyr t ca Ser gcc Al a ttg Leu cgt Ar g gcc Al a gca Al a cag G n 140 gct Al a tgg Tr p at g Met gaa G u ggt G0y 220 ct g Leu ttc Phe ct g Leu 45 agt Ser at g Met gcg Al a ggt G y gtt Val 125 ggt G y ttg Leu gcg Al a ttc Phe cat Hi s 205 gga Gy at c SIle ctt Leu cct Pr o gt g Val gt g Val ctt Leu gca Al a 110 ctt Leu gtt Val ggt Gy tgg Tr p agc Ser 190 gt g Val cct Pr o gca Al a 15 gat Asp tcg Ser gt g Val at g Met t ac Tyr 95 acg Thr ggc G y gca Al a ggt Gy at g Met 175 tcg Ser cag G n cgt Ar g ggt G y gtt Val at c SIle gat Asp gt g Val 80 ccc Pr o at g Met ggc G y gtt Val gt g Val 160 ttc Phe gca Al a ggc G y tta Leu acggcgatcc ttcgaagtat atg ctg ctc atg cag 11! IVet Leu Leu IVet G n 1 gcg ttg att ccg ctg 16; Ala Leu lie Pro Leu gtc tcc atc atc ccc 21 Val Ser Ile Ile Pro aag cca tcc ggc aag Lys Pro Ser y Lys ggc ctg aag tat ttg G y Leu Lys Tyr Leu ctg gat ctt atc gcc Leu Asp Leu Ile Ala gag atc gca gaa gtg G u I le Al a G u Val 100 ctg gcg ttc atg tac Leu Ala Phe IVet Tyr 115 gtg ctg tct ggt tgg 49! Val Leu Ser G y Trp 130 tat tgg tgc atc atc 54 Tyr Trp Cys Ile Ile 145 gca att gtt gtc agc 59! Ala lle Val Val Ser 165 atc atc atg atg gtc 64; Ile Ile Vet Vet Val 180 gtt cga aac gct att 69 Val Arg Asn Ala lle 195 cga atc caa ggt gtg 73! Arg lie n y Val 210 gct gac gtc ctt cac 78 Ala Asp Val Leu His 225 ggt tgg gcc gct gag ccc ctc ggc gca ggt tgg acg gta tta tgg ggc Page 190 seql i st cor r ect ed. t xt G y Trp Ala Ala G u Pro Leu Gy Ala G y Trp Thr Val Leu Trp G y 230 235 240 245 gga gta gcg gtg gtt gta ctc act gca att tgt atg gtg gcg gtg cct G y Val Ala Val Val Val Leu Thr Ala lle Cys Met Val Ala Val Pro 250 255 260 aaa ttc tgg aaa tac gag aaa cca aaa att acc ggc atc taaatactta Lys Phe Trp Lys Tyr G u Lys Pro Lys Ile Thr Gy Ile 265 270 tccatgccca ttt <210> 170 <211> 274 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 170 IVet Leu Leu IVet 1 Ala Leu Ile Pro Val Ser Ile Ile Lys Pro Ser y G y Leu Lys Tyr Leu Asp Leu Ile G u Ile Ala G u 100 Leu Ala Phe IVet 115 Val Leu Ser G y 130 Tyr Trp Cys Ile 145 Ala lle Val Val Ile lle Iet IVet 180 Val Arg Asn Ala 195 Arg lie Gn y 210 Al a Asp Val Leu 225 Thr Val Leu Trp G n 5 Leu Pr o Lys Leu Al a Val Tyr Tr p SIle Ser 165 Val l Ie Val Hi s Sy 245 Thr G y Al a SIle Thr Val Al a 70 Met Asn Ser Val Al a 150 Pr o SI e Leu Tr p Sy 230 Sy Sy Leu bet 55 Sy SIle Phe Ser Al a 135 Tr p Sy Sy G n SIle 215 Tr p Val Phe Tr p 40 Lys G n Phe Sy bet 120 Ar g Sy Al a Sy G n 200 I le Al a Al a I e Val y 10 G y Trp Leu 25 Al a Val Tr p Al a G y Phe Pro Val Leu G y let Pro 90 G y y Asp 105 Ala Val y I e Ser Ar g Al a Al a Val 155 Val Thr Al a 170 IVet Al a Asp 185 Ser Al a Al a Val Val Val Ala G u Pr o 235 Val Val Val 250 Pr o Tyr Ser Al a Leu Ar g Al a Al a G n 140 Al a Tr p Met G u Sy 220 Leu Leu Phe Leu Ser bet Al a Sy Val 125 Sy Leu Al a Phe Hi s 205 Sy Sy SIle Leu Pr o Val Val Leu Al a 110 Leu Val Sy Tr p Ser 190 Val Pr o Al a Al a Asp Ser Val bet Tyr Thr Sy Al a Sy bet 175 Ser a n Ar g Sy Leu Thr Ala lie Cys 255 Page 191 [Vb t ValI A seqI i st car r ect ed. t xt a Val Pro Lys Phe Tr p Lys Tyr G31u Lys Pro Lys I Ie Thr 260 265 270 G31y I Ie <210O> 171 <21 1> 549 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 <222> <223> OIDS 101). (526) FRXA01 937 <400> 171 gcgcggt gac accacagccg t t gt cagcgg cgct t ggt ct gt ggaggat c gccgaggt t a ct aacaaat a ggcccaacaa agaggtct aa gctct acct g gt g ValI 1 agt t tc cga Ser Phe Ar g at t t tc gct gac Il e Phe Al aAsp ac c Thr aga ccg ctg aaa Ar g Pr o Leu Lys g aa G u 15 ccg gcc t tc aaa Pr o Ala Phe Lys cgc ct C Arg Leu t gg ct t ggc Tr p Leu G31y gcc gt t ccg Ala Val Pro gt t gcc acc gt c Val A a Thr Val at t lIe ggt gcc caa tt a G3 y A a G3 n Le u act gt t gt t Thr Val Val ggc t at gt g G3 y Ty r Val gt g cag at t t ac Val On lieTyr c aa G31n 45 at g act ggg t cc I'bt Thr G3 y Ser t cc Ser ggc tt g a3 y Leu acc ggg ctt ttt Thr a3 y Leu Phe ggc a31y ct t at t cct tt g Leu I Ie Pro Leu at t t tt ggc ctt Il e Phe a y Leu t at Tyr ggt gga t cc at t a31y a31y Se r I Ie gcg A a 75 gat gct t tt gat Asp A a Phe Asp aaa Lys cgc at c gt g ct g Ar g I Ie Val Leu t gc acc acg at c Cys Thr Thr I Ie ggc a31y at g t gt gt c acc I'bt Cys Val Thr gcc ggt t tt t gg Ala a31y Ph e Tr p gt g ct g Val Leu 1 00 t tt tc t Phe Ser acc att tt a Thr I Ie Leu tta cag cag Leu a3 n a3 n 120 aat gag aat at t Asn a uAsn Il e t gg Tr p 110 ct c ct g t ta at a Leu Leu Leu Il e gca t tt t tc gcg A a Phe Phe A a aat caa ccc acc As n a31n Pr o Th r cga Ar g 130 acg gcg at c Th r A a I I e ct t cga Leu Arg 135 g aa agt at t t tg ccg Ser lie Leu Pro at t gat caa I I e Asp a3 n 140 t aagcgt cgg caacat cact <210O> <211 <21 2> <21 3> 1 72 142 PmT Cor ynebact er i urn gi ut anm cumn Page 192 seql i st cor r ec <400> 172 Val Ser Phe Arg Asp Ile Phe Ala Asp Thr Arg 1 5 10 Ala Phe Lys Arg Leu Trp Leu y Asn Val Al a G n Leu Thr Val Val Al a Val Pro Val G n Ile 40 Ser Ser y Tyr Val G y Leu Thr y Leu Phe 55 Val lie Phe y Leu Tyr Gy y Ser Ile Al a 70 Arg li e Val Leu I I e Cys Thr Thr I I e G y IVet 90 Sy Phe Trp Val Leu Thr Ile Leu y Asn G u 100 105 Leu Ile Asn Phe Ser Leu n G n Ala Phe Phe 115 120 Thr Arg Thr Ala l e Leu Arg Ser Ile Leu Pro 130 135 <210> 173 <211> 1242 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1219) <223> RXN01010 <400> 173 gtgccaaagc gtttcctgta aaacgcataa ccccgaatac aaaagatctg gcagggggtt taggcataga ttaggaactt ted. txt Pro Leu Thr Val Tyr G n G y Leu Asp Al a Cys Val Asn Il e Al a Val 125 Ile Asp 140 G u Gy Thr Pr o Asp Thr Leu G n at g Met gct Al a gt c Val acc Thr aag Lys ct c Leu gcc Al a t ca Ser cag G n at g Met gt g Val gtt Val ttg Leu gt c Val gcg Al a ttt Phe gcg Al a cca Pr o gt c Val tt g Leu ct g Leu tcg Ser gct Al a 75 tcg Ser gga Sy ccg Pr o acc Thr ggg Sy t at Tyr tgg Tr p ggc Sy gaa G u at g Met 45 cgc Ar g at t SIle ggg G y ttt Phe ttt Phe acc Thr at c SIle gt c Val ct g Leu gtg ggg Val G y 15 gca gac Al a Asp gca t ac Al a Tyr acc aga Thr Arg aca ct g Thr Leu 80 ttt atg Phe IVet Page 193 cccctgtttc cagatccaaa atg aag aaa ctg caa IVet Lys Lys Leu G n 1 ccg ttt act ggc caa Pro Phe Thr y G n acc ttt gat atc agt Thr Phe Asp lie Ser ttg ttg ccc ttt gcc Leu Leu Pro Phe Ala aag atc cat ccg cat Lys Ile His Pro His cca ctt gcg ctg ttg Pro Leu Ala Leu Leu gct gcg tat gcc acg Ala Ala Tyr Ala Thr INO seql i st cor r ect ed. t xt 95 100 att ggt atc gct aat gca ttt acc act ccg gtg ctg caa att atg ttg 451 I e Gy I e Ala Asn Ala Phe Thr Thr Pro Val Leu n Ile IVet Leu 105 110 115 4 cgt gag ctt gtt ccg ccg cgt tct ttg ggt aag gca ttg ggc acc tat 499 SArg G u Leu Val Pro Pro Arg Ser Leu Gy Lys Ala Leu Gy Thr Tyr 120 125 130 gct gcg atg caa tca ctc ggc atg ttg tcg gcg cca ctg atc gca ggt 547 Al a Al a Iet G n Ser Leu G y IVet Leu Ser Al a Pro Leu I e Al a G y 135 140 145 00 gtg tct tcg gtg gtg tcg tgg agg ttg acc ttc ctg gtc act gca gca 595 0 Val Ser Ser Val Val Ser Trp Arg Leu Thr Phe Leu Val Thr Ala Ala S150 155 160 165 (N gcg tca ctg ttt att ttg gtg gcg cga ctc ccc gtt gtt cca cca cca 643 Ala Ser Leu Phe lie Leu Val Ala Arg Leu Pro Val Val Pro Pro Pro 170 175 180 (N tca gca tcg aag caa aac gtt agt ggc aag gtg cag tgg gga ccg acc 691 Ser Ala Ser Lys G n Asn Val Ser y Lys Val G n Trp y Pro Thr 185 190 195 atc atc cac atg gtt tcc ggc ttt gt gtgg ggc atc ggc atc atc ggc 739 IIe IIe His IVet Val Ser G y Phe Val Val G y Ile G y IIe Ile G y 200 205 210 att gga ttc atg aca tcg ctg cac gtt ggc gag caa ttc gga ctt gat 787 IIe G y Phe IVet Thr Ser Leu His Val G y G u G n Phe G y Leu Asp 215 220 225 gct gca gcg cgt ggt ttg gtg gtc atg tgt ggt ggc ctg gct gcg ttc 835 Al a Al a Al a Arg G y Leu Val Val Vet Cys G y G y Leu Al a Al a Phe 230 235 240 245 ttt gcc tcc cgc aag att ggc gat ttg gca gac aaa ttt ggt gtg cgc 883 Phe Ala Ser Arg Lys I e y Asp Leu Ala Asp Lys Phe y Val Arg 250 255 260 gcg gtg ctc att gtc agt gct gtc atc ggt acc atc gca ctc gca ctg 931 Ala Val Leu lie Val Ser Ala Val Ile y Thr Ile Ala Leu Ala Leu 265 270 275 ctg ccg atc gca ccg tgg atc att gtg gtg gcc gta ctg tgg gcc ttc 979 Leu Pro Ile Ala Pro Trp Ile Ile Val Val Ala Val Leu Trp Ala Phe 280 285 290 gca gta gca gca gca caa gga atc caa gca acc gtc aac ttg gct gtc 1027 Ala Val Ala Ala Ala n y Ile n Ala Thr Val Asn Leu Ala Val 295 300 305 atc gga agc ccc ggt gga tca tcg ctg ctt tct acc gtg cag gct ttc 1075 Ile y Ser Pro y G y Ser Ser Leu Leu Ser Thr Val G n Ala Phe 310 315 320 325 cga ttc ttc gga tca gcg gca gca cca gtg aca ttc ctt cct atc tat 1123 Arg Phe Phe y Ser Al a Ala Ala Pro Val Thr Phe Leu Pro lie Tyr 330 335 340 atg ggc atc ggc tcg ggg gcg ttt tgg gtc agc gcg gta gcg ctg ttc 1171 e t G y I le G y Ser G y Al a Phe Trp Val Ser Al a Val Al a Leu Phe 345 350 355 ttc gtt gcc atc gcc cag tgg ctc aac ccg cag cgg gtg gag cgg ggc 1219 Phe Val Ala Ile Ala G n Trp Leu Asn Pro G n Arg Val G u Arg y Page 194 seqI i st car r ect ed. t xt 360 365 370 t gagggagac gt cgagaagc gt c <210O> 174 <21 1> 373 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1242 <400> 174 I'bt Lys Lys Leu 03 n I'bt Pr o Th r Le u Ly s Pr o A a Le u A a Pr o 145 Le u ValI O31n I Ie O31n 225 G31y Ly s I Ie ValI Th r As p Pr o Hi s A a Ty r I Ie 115 G31y I Ie Th r Pr a G31y 1 95 li1e G31y A a G31y Le u 275 Tr p O31n Ser A a Hi s Le u Th r Le u Ty r G31y A a 1 65 Pr a Th r G31y As p Ph e 245 Ar g Le u Ph e A a ValI Th r Ly s 70 Le u I Ie Ar g A a ValI 150 A a Ser le le A a 230 Ph e A a Le u A a Pr a Le u Ser vb t ValI Le u G31y O31u A a 135 Ser Ser A a I Ie G31y 215 A a A a ValI Pr a ValI 295 I Ie ValI A a Le u O31n Th r A a 1 ValI O31n ValI Ph e Ly s 1 vb t vb t Ar g Ar g I Ie 265 A a A a Le u 10 ValI A a Ph e A a Pr a 90 As n Pr a Ser ValI I Ie 1 70 O31n ValI Th r O31y Ly s 250 ValI Pr a ValI Le u Le u Ser A a 75 Ser A a Pr a Le u Ser 1 55 Le u As n Ser Ser Le u 235 li1e Ser Tr p O31y Pr a Th r O31y Ty r Tr p Ph e Ar g O31y 140 Tr p ValI ValI O31y Le u 220 ValI O31y A a I Ie O31y O31u vb t Ar g I Ie O31y Th r Ser 125 vb t Ar g A a Ser Ph e 205 Hi s ValI As p ValI I Ie 285 ValI A a A a Th r Th r Ph e Pr a O31y Ser Th r Le u 1 Ly s ValI O31y cys A a 255 O31y ValI O31y As p Ty r Ar g Le u vb t ValI Ly s A a Ph e 1 Pr a ValI O31y O31u O31y 240 As p Th r A a A a 03 n 03 y 300 Il e O n Al aThr Page 195 seqi i st car r ec Val Asn Leu Al a Val I Ie G31y Ser Pr o 31y G31y 305 310 315 Thr Val O31n Al a Phe Ar g Phe Phe G31y Ser Al a 325 330 Phe Leu Pr o I Ie Ty r 'b t G31y IIe G31y Ser G31y 340 345 Al a Val Al a Leu Phe Phe Val Ala I Ie Al a O31n 355 360 Ar g Val O31u Ar g O31y 370 <210O> 175 <21 1> 871 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (871) <223> FRXA01010 <400> 175 gt gccaaagc gt t tcct gt a aaacgcat aa ccccgaat ac aaaagat ct g gcagggggt t t aggcat aga t taggaact t ted. t xt Ser Ser Leu Leu Ser 320 Ala Ala Pr o Val Thr 335 Al a Phe Tr p Val Ser 350 Trp Leu As n Pr o O31n 365 cccct gt t tc cagat ccaaa at g vb t gct Al a gt c ValI ac c Th r aag Ly s ct c Le u at t I Ie cgt Ar g gct Al a gcc Al a t ca Ser c ag O31n at g vb t gt g ValI gt t ValI at c ct t Le u 120 at g tt g Le u gt c ValI gcg Al a tt t Ph e gcg Al a cca Pr o aat As n ccg Pr o t ca Ser gt c ValI t t g Le u ct g Le u t cg Ser gct Al a 75 t cg Ser gca Al a ccg Pr o ct c Le u gga O31y ccg Pr a ac C Th r ggg O31y t at Ty r t gg Tr p tt t Ph e cgt Ar g ggc G31y 140 ggc O31y g aa O31u at g vb t 45 cgc Ar g at t O31y ac C Th r t ct Ser 125 at g tt t Ph e tt t Ph e ac C Th r at c I Ie gt c ValI ct g Le u act Th r 110 tt g Le u tt g Le u gt g ValI 15 gca Al a gca Al a ac c Th r ac a Th r tt t Ph e 95 ccg Pr a ggt (31y ggg O31y g ac As p t ac Ty r ag a Ar g ct g Le u 80 at g vb t gt g ValI aag Ly s at g vb t 1 ccg Pr a ac c Th r tt g Le u aag Ly s cca Pr a gct Al a ct g Le u gca Al a aag aaa ct g Lys Lys Leu tt t Ph e tt t Ph e tt g Le u at c I Ie ct t Le u gcg Al a c aa O31n tt g Le u 130 ggc O31y at c Ph e ccg Pr a ct g Le u gcc Al a 1 00 at g vb t ac c Th r c aa O31n c aa O31n agt Ser gcc Al a cat Hi s tt g Le u ac g Th r tt g Le u t at Ty r t cg gcg cca ct g at c gca ggt Ser Ala Pro Leu lie Ala Gly 145 Page 196 gt g Val 150 gcg Al a t ca Ser at c SIle at t SIle act Thr 230 ttt Phe tcg Ser ct g Leu ttg Leu cac Hi s 200 ttc Phe gcg Al a t cc Ser gt g Val at t SIle 170 caa G n gtt Val aca Thr ggt Sy aag Lys 250 tcg Ser 155 tt g Leu aac Asn t cc Ser tcg Ser tt g Leu 235 at a SIle tgg Tr p gt g Val gtt Val ggc G y ct g Leu 220 gt g Val ggc Gy seql i st cor r ect ed. t xt agg ttg acc ttc ctg gtc Arg Leu Thr Phe Leu Val 160 gcg cga ctc ccc gtt gtt Ala Arg Leu Pro Val Val 175 agt ggc aag gtg cag tgg Ser Gy Lys Val G n Trp 190 ttt gtg gtg ggc atc ggc Phe Val Val G y lIe y 205 210 cac gtt ggc gag caa ttc His Val G y u n Phe 225 gtc atg tgt ggt ggc cgg Val IVet Cys G y y Arg 240 gat ttg gca gac aaa Asp Leu Ala Asp Lys 255 act Thr cca Pr o gga G y 195 at c SIle gga G y gct Al a gca Al a cca Pr o 180 ccg Pr o at c SIle ctt Leu gcg Al a gca Al a 165 cca Pr o acc Thr ggc G y aat Asn ttc Phe 245 <210> 176 <211> 257 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 176 IVet Lys Lys Leu G n IVet Pro Ala lie 1 5 Pro Phe Thr y G n Ala Leu Ser Val Thr Phe Asp lie Ser Val Ser G n Ala 40 Leu Leu Pro Phe Ala Thr IVet IVet Leu Lys lie His Pro His Lys Val Val G n 70 Pro Leu Ala Leu Leu Leu Leu Val Thr Al a Ala Tyr Al a Thr lie y ile Al a 100 105 Leu n lie IVet Leu Arg G u Leu Val 115 120 Ala Leu y Thr Tyr Al a Ala IVet G n 130 135 Pro Leu Ile Ala y Val Ser Ser Val 145 150 Leu Val Thr Ala Ala Al a Ser Leu Phe Leu 10 Val Al a Phe Al a Pr o 90 Asn Pr o Ser Val Val Leu Leu Ser Al a 75 Ser Al a Pr o Leu Ser 155 Sy G u Met Ar g l Ie Sy Thr Ser 125 tA Ar g Val G y Al a Asp Al a Tyr Thr Arg Thr Leu Phe Met Pro Val G y Lys Ser Al a Thr Phe 160 Leu Pro 175 I e Leu Val Ala Arg 170 Page 197 seqI i st car r ect ed. t xt Val Val Pro Pro Pro Ser Ala Leu Lys O31n Asn Val Ser O31y Lys Val 180 185 190 O31n Tr p O31y Pr o Thr I Ie I Ie Hi s I'b t Val Ser O31y Phe Val Val O31y 195 200 205 1 1e (1y I Ie I Ie (1y I Ie (1y Phe 'b t Thr Ser Leu Hi s Val O31y (1u 210 215 220 O31n Phe O31y Leu As n Thr Ala Ala Ar g O31y Leu Val Val I'b t Cys G31y 225 230 235 240 G31y Ar g Ala Ala Phe Phe Ala Ser Ar g Lys Ilie G31y Asp Leu Ala Asp 245 250 255 Ly s <210O> 177 <21 1> 1266 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1243) <223> RXN03142 <400> 177 gcaatttggc caatcaacaa cataggagga aaaaccacac ccat cgcaac caacggcact ct gcgt ggcg gt caccgcaa gaacggacat gt t cact cca t gg Tr p gt c ValI gt c ValI gt g ValI ct c Le u gct A a t ct Ser at t I Ie gt c ValI gcg A a tt t Ph e gcg A a tt t Ph e t ca Ser t ct Ser gca A a 120 tt g Le u t at Tyr gcc A a t gg Tr p ggC G31y cca Pr a gct A a cgt Ar g acc Thr gcg A a ag c Ser ac g Thr t t g Le u 75 gcg A a t cc Ser ag g Ar g c ag O31n at g vb t t cc Ser t cc Ser g aa O31u ct g Le u ac c Thr gca A a t ac Tyr g aa O31u tt t Ph e 45 g ac As p gcg A a gt t ValI gct A a gag (31u 125 tt g Le u ag c Ser gt t ValI cgt Ar g gt a ValI gct A a gt g ValI 110 ggc (31y tt c Ph e gcc A a ggc O31y ggt O31y t ca Ser cgt Ar g gca A a ggc (31y gt g ValI 1 t ac Tyr gt t ValI gca A a aaa Lys5 ct a Le u tt t Ph e ct t Le u t ac Tyr tt c Ph e t ca Ser ac c Thr aaa Lys5 tt c Ph e gt t ValI gt g ValI t tt at t tt g Phe Il e Leu at c lIe gcc A a gct A a at c I Ie at t I Ie ggt O31y 1 00 t cc Se r ttc gcg ctc gga Phe A a Leu 03 y 130 Page 198 seql i st cor r ect ed. t xt t cc aca c Ser Thr L 135 gat gac t Asp Asp P 150 gtt ttc g Val Phe G aag aat g Lys Asn A gtt gtg c Val Val H 2 gga ctg g G y Leu A 215 gac act g Asp Thr G 230 gtt gcg a Val Ala N cat ggt g Hi s G y A gcg ctg g Al a Leu G 2 gct ggc g Al a y A 295 caa gcc a G n Al a 310 att tct a Ile Ser T at c ct g c Ile Leu L gca gct t Al a Al a L 3 gct ttg g Al a Leu G 375 <210> 178 <211> 381 tg gct aca gct eu Ala Thr Al a tc he gc ly cg I a ac is 00 ct I a gc ly tg et ac sp gg ly 80 ca I a tt le cg hr tg eu tg eu 60 gt ly t ac Tyr at c SIle 170 cac Hi s tcg Ser gca Al a acc Thr gt c Val 250 ccg Pr o at g Met acc Thr gt c Val ttc Phe 330 gga G y gca Al a ttc Phe 140 acc Thr acc Thr aga Ar g at g Met gt g Val 220 gga G y cgt Ar g at t SIle ttg Leu ttg Leu 300 t ca Ser ttc Phe gtt Val at t l Ie ggc cca gca att Sy Pro Ala lle ct g Leu gtt Val gt a Val ct c Leu 205 at c SIle gcg Al a ttc Phe t ac Tyr gcg Al a 285 ggc G y gtt Val acc Thr gca Al a gtt Val 365 ttc Phe ttg Leu aag Lys 190 at t SIle acc Thr ggt G y ttc Phe ttc Phe 270 act Thr t at Tyr cca Pr o gac Asp gcg Al a 350 gt g Val tgg Tr p at c SIle 175 cca Pr o gga G y ttc Phe ctt Leu ctt Leu 255 ggt G y gaa G u ggc G y agc Ser at c SIle 335 acc Thr gcg Al a at t SIle 160 cgc Ar g gt c Val ttc Phe ct c Leu ttc Phe 240 gga G y ttg Leu gac Asp acc Thr act Thr 320 ggc Sy gga G y ggc G y gcg ctg ttt Ala Leu Phe 145 acc act gcg Thr Thr Al a aag ccg gag Lys Pro G u tgg tct atc Trp Ser Ile 195 ttc atg ctc Phe IVet Leu 210 aac ggc ttc Asn G y Phe 225 ttt atc gct Phe Ile Al a cgc att cag Arg lie n atc agc ttc Ile Ser Phe 275 tgg cac at t Trp His Ile 290 atc atg ccg Ile IVet Pro 305 cag gtt ggt Sn Val y att ggc tta Ile G y Leu tac aac gt c Tyr Asn Val 355 gtg ctc tac Val Leu Tyr 370 gtt Val acc Thr ttc Phe 180 aag Lys gct Al a gcg Al a t at Tyr gac Asp 260 gcc Al a gtt Val gcc Al a t cc Ser ggc G y 340 at g Met ct g Leu at c SIe agt Ser 165 at t SIe act Thr gt c Val caa G n gcg Al a 245 aaa Lys ct c Leu ct c Leu gca Al a ggt G y 325 cca Pr o t ac Tyr gtt Val 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1266 agg aaa gct agc cac Arg Lys Ala Ser His 380 taagttagag cattttattg agc Page 199 seqI i st car r ect ed. t xt <21 2> PmT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 178 Val Phe I Ie Ty r ValI A a Ly s Le u Ph e Le u Ty r A a 145 Th r Ly s Tr p Ph e As n 225 Ph e Ar g I Ie Tr p I Ie 305 O31n Le u G31u ValI O31n Ty r Hi s G n 115 A a Ph e A a O31u I Ie 1 95 Le u Ph e A a O31n Ph e 275 li1e Pr o G31y Leu 03 y 1 1 e Thr A a A a Ala Ar g I I e Leu I Ie Pr o 03 y Phe 1 00 Ser Val Leu 03 y Val I Ie Thr Ser 1 65 Phe I I e 1 80 Lys Thr A a Val A a 03 n Ty r A a 245 As p Lys 260 A a Leu Val Leu A a A a Ser 03 y 325 Trp Leu Val [Vb t Val G31y Val Phe Leu I I e 70 A a A a Ser Tyr I Ie Pr o Ser Thr 135 As p As p 150 Val Phe Lys As n Val Val 03 y Leu 215 As p Thr 230 Val Ala Hi s 03 y A a Leu A a 03 y 295 O3 n A a 310 1 1 e Ser ValI A a Ph e 40 A a Ph e Ser Ser A a 120 Le u Ph e G31y A a Hi s 200 A a G31y vb t As p G31y 280 A a I Ie Thr As n Le u 25 A a G31y ValI Le u Le u 1 05 Ser A a As n Le u O31u 1 85 Pr o Tyr Le u Le u As n 265 Le u Le u A a Le u Thr A a Ser Thr Le u A a Ser Ar g A a As n 1 Le u G31y ValI G31y A a 235 vb t ValI A a G31y As p 315 Le u O31n vb t Ser Ser O31u Le u Thr A a Ph e 140 Thr Thr Ar g vb t ValI 220 G31y Ar g I Ie Le u Le u 300 Ser Ph e Tyr O31u Ph e As p A a ValI A a G u 125 G31y Le u ValI ValI Le u 205 li1e A a Ph e Tyr A a 285 G31y ValI Thr Le u Ser ValI Ar g ValI A a ValI 110 G y Pr o Ph e Le u Ly s 1 li1e Thr 0Gy Ph e Ph e 270 Thr Tyr Pr o As p Ser Ph e I Ie Ph e A a ValI vb t Thr A a Tr p I Ie 1 Pr a G31y Ph e Le u Le u 255 G31y O31u G31y Se r I Ie 335 Ile(3y Leu Oy Pro Ilie Leu Leu Oy y LeuVal AiaAiaThr Gy Page 200 seql i st cor r ect ed. t xt 340 345 350 Tyr Asn Val Met Tyr Ala Ala Leu Ala Ala Val lie Val Val Ala y 355 360 365 Val Leu Tyr Leu Val Ala Leu G y Arg Lys Ala Ser His 370 375 380 <210> 179 <211> 914 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> FRXA01150 <400> 179 cca gct gcc Pro Ala Al a 1 ttt tct tat Phe Ser Tyr gtg att cct Val Ile Pro gga t cc aca G y Ser Thr at c gat gac Ile Asp Asp agt gtt ttc Ser Val Phe at t aag aat I e Lys Asn act gtt gtg Thr Val Val 115 gt c gga ct g Val G y Leu 130 caa gac act G n Asp Thr 145 gcg gtt gcg Al a Val Al a aaa cat ggt Lys Hi s G y ct a Leu 5 ctt Leu agc Ser gct Al a aac Asn ct g Leu gaa G u cca Pr o t ac Tyr ct c Leu ct g Leu 165 aac Asn cca gcg ctg gtt gct gtg cgt ttt gtt Pro Ala Leu Val Ala Val Arg Phe Val 10 gct Al a cgt Ar g aca Thr tac Tyr 70 at c SIle cac Hi s tcg Ser gca Al a acc Thr 150 gt c Val ccg Pr o t cc Ser agg Ar g gct Al a 55 aac Asn ct c Leu ggc Gy gt c Val ggc Gy 135 gcc Al a at g Met gtt Val gct Al a gag G u ggc Gy ct g Leu gtt Val gt a Val 105 ct c Leu at c SIle gcg Al a ttc Phe t ac Tyr 185 at g Met acc Thr gca Al a tgg Tr p 75 at c SIle cca Pr o gga Gy ttc Phe ctt Leu 155 ctt Leu ggt Gy gca Al a ggc Gy at t I e at t SIle cgc Ar g gt c Val ttc Phe ct c Leu 140 ttc Phe gga Gy ttg Leu ctt Leu t ac Tyr gcg Al a acc Thr aag Lys tgg Tr p ttc Phe 125 aac Asn ttt Phe cgc Ar g at c SIle cac ggt Hi s G y cag tcc G n Ser gcg ctc Al a Leu ttt gtt Phe Val gcg acc Al a Thr gag ttc G u Phe atc aag I e Lys ct c gct Leu Al a ttc gcg Phe Al a gct tat Al a Tyr 160 cag gac Sn Asp 175 ttc gcc Phe Al a Page 201 seql i ctc gcg ctg ggg ctt atg gct ttg gcg Leu Al a Leu G y Leu IVet Al a Leu Al a 195 200 ctc gct ggc gca ctc acc ggt ttg ggc Leu Al a G y Al a Leu Thr G y Leu G y 210 215 gca caa gcc att gct gtc gat tca gtt Ala n Ala lie Ala Val Asp Ser Val 225 230 ggt att tct acg ctt ttc ctg ttc acc Sy Ile Ser Thr Leu Phe Leu Phe Thr 245 cca atc ctg ctg ggt gga ttg gtt gca Pro lie Leu Leu y G y Leu Val Ala 260 265 tac gca gct ttg gcc gca gtg att gtt Tyr Ala Ala Leu Ala Ala Val lie Val 275 280 gtt gct ttg ggt agg aaa gct agc cac Val Al a Leu G y Arg Lys Al a Ser His 290 295 agc <210> 180 <211> 297 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 180 Pro Ala Ala Ser Leu Pro Ala Leu Val 1 5 Phe Ser Tyr Ser Leu Al a Ser Thr Ala Val Ile Pro Ala Ser Arg Arg Ala G u 40 Sy Ser Thr Leu Ala Thr Ala Phe y 55 Ile Asp Asp Phe Asn Tyr Asn Thr Leu 70 Ser Val Phe y Leu Ile Leu Thr Val I Ie Lys Asn Ala G u His G y Arg Val 100 105 Thr Val Val His Pro Ser Val bet Leu 115 120 Val y Leu Ala Tyr Al a y Val lie 130 135 G n Asp Thr G y Leu Thr Al a y Al a 145 150 st cor r ect ed. t xt act gaa gac tgg cac att Thr u Asp Trp His lie 205 tat ggc acc atc atg ccg Tyr G y Thr I e Met Pro 220 cca agc act cag gtt ggt Pro Ser Thr G n Val y 235 gac atc ggc att ggc tta Asp Ile G y Ile G y Leu 250 255 gcg acc gga tac aac gtc Al a Thr G y Tyr Asn Val 270 gtg gcg ggc gtg ctc tac Val Ala y Val Leu Tyr 285 taagttagag cattttattg Al a 10 Val Gy Pr o Phe Leu 90 Lys SIle Thr Gy Val et Thr Al a Tr p 75 I le Pr o Gy Phe Leu 155 Ar g Al a Gy l Ie I le Ar g Val Phe Leu 140 Phe Phe Leu Tyr Al a Thr Lys Tr p Phe 125 Asn Phe Hi s G n Al a Phe Al a G u I e Leu Phe Al a Gy Ser Leu Val Thr Phe Lys Al a Al a Tyr 160 Ala Val Ala IVet Leu Val IVet Arg Phe Phe Leu y Arg lie G n Asp Page 202 seql i st cor r ec 165 170 Lys His G y Asp Asn Pro Val lie Tyr Phe G y 180 185 Leu Al a Leu G y Leu ebt Al a Leu Al a Thr G u 195 200 Leu Al a y Al a Leu Thr G y Leu G y Tyr G y 210 215 Ala G n Ala lie Ala Val Asp Ser Val Pro Ser 225 230 235 Sy Ile Ser Thr Leu Phe Leu Phe Thr Asp Ile 245 250 Pro lie Leu Leu y G y Leu Val Ala Ala Thr 260 265 Tyr Ala Ala Leu Ala Ala Val lie Val Val Ala 275 280 Val Ala Leu G y Arg Lys Ala Ser His 290 295 <210> 181 <211> 1341 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1318) <223> RXN02964 <400> 181 ttttatatcc tagcaagggt gttgcatgat gcaataaacg aaaattgcat gatgcaataa tttcgattta aaggagaaca ted. txt Leu II e Asp Trp 205 Thr Ile 220 Thr G n G y lle G y Tyr G y Val 285 175 Phe SIle Pr o Sy Leu 255 Val Tyr Al a Val Al a Ser 240 Sy LMt Leu gaa G u ctt Leu t ac Tyr t cc Ser t at Tyr ct a Leu aaa Lys cag G n at c SIle t ca Ser at t SIle act Thr ttt Phe ct g Leu gaa G u gtt Val cgt Ar g at t SIle aca Thr gt g Val ttt Phe at c SIle ttt Phe 75 gcg Al a cca Pr o ttc Phe caa G n ggc G y ggt G y tgt Cys acg Thr t ac Tyr gcc Al a 45 gca Al a cga Ar g gcc Al a ttt Phe ttc Phe tct Ser gt c Val cgc Ar g ttg Leu gt c at g Val MVet 15 ct c at c Leu II e gaa gt a G u Val ttt tcc Phe Ser aag att Lys II e 80 tac ctt Tyr Leu 95 Page 203 tggtagtttg tgttcataac gtg tcc gta gct gaa Val Ser Val Ala G u 1 gga tgg ttt gcc aac Sy Trp Phe Ala Asn acc acc atg gct ttg Thr Thr IVet Ala Leu gaa gct ggc ttc gca G u Al a y Phe Al a agg ttt ttc tcc ggc Arg Phe Phe Ser y gtg ctc atc tca gtc Val Leu Ile Ser Val ccc atc gaa tca ttg Pro lie u Ser Leu 100 seql i st cor r ect ed. t xt ttc ctc cac aat att aa cca ttg cta tac Pro Leu Leu Tyr 105 gca aac agg Al a Asn Ar g a Phe Leu Hi s G y Val y gct Al a cga Ar g gca Al a 150 t ac Tyr at c SIle ggg Gy at c SIle gt c Val 230 ggt Gy cgc Ar g at t SIle ttt Phe ct g Leu 310 gt a Val ttt Phe tct Ser acc Thr 120 t cc Ser ctt Leu at g Met gt c Val cca Pr o 200 at c SIle gcc Al a gga Gy ttc Phe ttt Phe 280 act Thr t ac Tyr gac Asp gac Asp gca Al a 360 at c SIle ggt Gy cca Pr o ttt Phe 170 ttc Phe aag Lys at c SIle at c l Ie ttc Phe 250 ggc Gy ttg Leu aac Asn act Thr acc Thr 330 ggt Gy ggt Gy at g Mt act Thr gcc Al a 155 at c SIle at g bet tt c Phe ttt Phe aac Asn 235 tt c Phe aaa Lys tt c Phe tgg Tr p tt g Leu 315 gaa G u ttt Phe tt c Phe gca Al a ggt Gy 140 ct a Leu gt g Val t ac Tyr agc Ser at c SIle 220 gca Al a at t SIle ct g Leu ttc Phe cac Hi s 300 at g Met ttc Phe ggc gay gga Gy at g Mbt 125 t ac Tyr gca Al a gt c Val ttt Phe ttc Phe 205 ttg Leu ttt Phe gcc Al a cag G n ttc Phe 285 gtt Val cca Pr o ggt Gy ttt Phe cct Pr o 365 110 gt c Val ct g Leu ctt Leu ttg Leu aag Lys 190 aaa Lys ctt Leu gct Al a t ac Tyr gac Asp 270 gtt Val gt g Val gca Al a acg Thr gga Gy 350 at g Met cag G n gca Al a ttt Phe gca Al a 175 acc Thr tct Ser at t SIle gaa G u gca Al a 255 cgt Ar g at t SIle ttg Leu gt g Val gcc Al a 335 cct Pr o t at Tyr gag G u ttg Leu gt c Val 160 acc Thr agc Ser at t SIle tgc Cys gaa G u 240 gt a Val cgc Ar g t cc Ser t cc Ser cag G n 320 ttc Phe at t SIle gca Al a ct c Leu ggc Gy 145 ct a Leu tcg Ser gac Asp at g Met t ttt Phe 225 cgc Ar g t ca Ser gga Gy ttg Leu gga Gy 305 t cc Ser t cc Ser at c SIle gca Al a at t SIle 130 act Thr gga Gy gt c Val cct Pr o aac Asn 210 gct Al a gat Asp at g Met gac Asp acg Thr 290 gt c Val at c SIle act Thr ct g Leu ct g Leu 370 tac gct ttt Tyr Al a Phe 115 cca gcg t ca Pr o Al a Ser acc gtt t ct Thr Val Ser aca ttt gat Thr Phe Asp 165 atc tct ttg Ile Ser Leu 180 gag cct tct G u Pro Ser 195 cca aag at c Pro Lys Ile tac tct ggc Tyr Ser G y ctg att acg Leu Ile Thr 245 ttt gtg atg Phe Val Met 260 aac gtc gtt Asn Val Val 275 att ttg tcc Ile Leu Ser att gca ggt i e Al a G y gct gtt ggt Ala Val y 325 ttg ttc ctg Leu Phe Leu 340 gga gca gtt G y Al a Val 355 gca ggt gt g Al a G y Val 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 204 seql i st cor r ect ed. t xt ggt gtg att gcc gga atc ttc tac ctg ttc aca cac gct cgc acc gat G y Val I I e Ala G y II e Phe Tyr Leu Phe Thr His Ala Arg Thr Asp 375 380 385 cga gct aag aat ggc ttt gtt aaa cac cca gag cct gtc gct tta gtt Arg Al a Lys Asn G y Phe Val Lys His Pro G u Pro Val Ala Leu Val 390 395 400 405 agc tagttctttc agctttccct ccc Ser <210> 182 <211> 406 <212> PRT <213> Corynebacter i um gl utam cum 1267 1315 1341 <400> 182 Val Ser Val 1 G y Trp Phe Thr Thr Met G u Al a G y Arg Phe Phe Val Leu II e Pro lie u Val G y Tyr 115 Leu Ile Pro 130 G y Thr Thr 145 Leu G y Thr Ser Val I e Asp Pro u 195 Iet Asn Pro 210 Phe Al a Tyr 225 Arg Asp Leu Al a G u 5 Al a Asn Al a Leu Phe Al a Ser G y Ser Val Ser Leu 100 Al a Phe Al a Ser Val Ser Phe Asp 165 Ser Leu 180 Pro Ser Lys II e Ser G y Ile Thr 245 Su G y Lys Leu Thr Pro Thr Phe Val Met Leu Tyr Ser Tyr 70 Leu Pr o Al a Ar g Al a 150 Ty r SIle Gy I e Val 230 Sy Phe Al a Ser 55 I le Val Leu Al a Ar g 135 Al a Asp Al a G u Pr o 215 I le Al a G n I e 40 Ser SIle Thr Leu Thr 120 Ser Leu let Val Pr o 200 I le Al a Sy Phe 25 Lys SIle Asp Thr Tyr 105 Al a G u Sy Leu Val 185 Al a Sy Tyr Leu Val Phe SIle Phe Al a Asn let Thr Al a 155 I le let Phe Phe Asn 235 Phe Phe G n Sy Sy Cys Ar g Al a Sy 140 Leu Val Tyr Ser SIle 220 Al a SIle Tyr Al a Al a Ar g Al a Phe et 125 Tyr Al a Val Phe Phe 205 Leu Phe Al a Phe Ser Val Ar g Leu Leu 110 Val Leu Leu Leu Lys 190 Lys Leu Al a Tyr Leu G u Phe Lys Tyr Hi s G n Al a Phe Al a 175 Thr Ser I e Su Al a 255 Ser IVet Phe Val IVet Arg Ser Phe Leu y Lys Leu G n Asp Arg Arg Page 205 Gy Leu Gy 305 Ser Ser SIle Al a Hi s 385 Pr o Asn 275 I e I e Al a Leu Gy 355 Al a Ar g Al a Val Ser Gy Gy 325 Leu Val Val Asp Val 405 Tyr Al a 295 Gy Val Val Al a Val 375 Al a seql i st cor r ect ed. t xt 265 Phe G y Leu Phe Phe Phe 280 285 Thr Ser Asn Trp His Val 300 Tyr y Thr Leu IVet Pro 315 Asp Lys Thr G u Phe G y 330 Asp Leu G y Phe G y Phe 345 Ala lie y Phe y Pro 360 365 lI e Al a y Il e Phe Tyr 380 Lys Asn y Phe Val Lys 395 270 Val Val Al a Thr Sy 350 Met Leu Hi s SIe Leu Val Al a 335 Pr o Tyr Phe Pr o <210> 183 <211> 1006 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1006) <223> FRXA02116 <400> 183 ttttatatcc tagcaagggt gttgcatgat gcaataaacg aaaattgcat gatgcaataa tttcgattta aaggagaaca gaa G u ctt Leu t ac Tyr t cc Ser t at Tyr ct a aaa Lys cag G n at c SIle t ca Ser at t SIle act ttt Phe ct g Leu gaa G u gtt Val cgt Ar g at t aca Thr gt g Val ttt Phe at c SIle ttt Phe 75 gcg cca Pr o ttc Phe caa G n ggc G y ggt Sty tgt acg Thr t ac Tyr gcc Al a 45 gca Al a cga Ar g gcc ttt Phe ttc Phe tct Ser gt c Val cgc Ar g ttg gt c at g Val MVet 15 ct c at c Leu II e gaa gt a G u Val ttt tcc Phe Ser aag att Lys II e 80 tac ctt Page tggtagtttg tgttcataac gtg tcc gta gct gaa Val Ser Val Ala G u 1 gga tgg ttt gcc aac Sy Trp Phe Ala Asn acc acc atg gct ttg Thr Thr IVet Ala Leu gaa gct ggc ttc gca G u Al a y Phe Al a agg ttt ttc tcc ggc Arg Phe Phe Ser y gtg ctc atc tca gtc Val Leu Ile Ser Val ccc atc gaa tca ttg Leu Val Thr Thr Ile Ala Cys Al seql i st cor r ect ed. t xt a Leu Tyr Leu Pro II 95 e G u Ser Leu 100 cca Pr o gct Al a cga Ar g gca Al a 150 t ac Tyr at c SIle ggg G y at c SIle gt c Val 230 ggt Sy cgc Ar g at t SIle ttt Phe ct a Leu acc Thr 120 t cc Ser ctt Leu at g let gt c Val cca Pr o 200 at c SIle gcc Al a gga G y ttc Phe ttt Phe 280 act Thr gca Al a at c SIle ggt Gy cca Pr o ttt Phe 170 ttc Phe aag Lys at c SIle at c l Ie ttc Phe 250 ggc G y ttg Leu aac Asn agg Ar g gca Al a ggt Gy 140 ct a Leu gt g Val t ac Tyr agc Ser at c SIle 220 gca Al a at t SIle ct g Leu ttc Phe cac Hi s 300 ct c Leu 110 gt c Val ct g Leu ctt Leu ttg Leu aag Lys 190 aaa Lys ctt Leu gct Al a t ac Tyr gac Asp 270 gtt Val gt g Val cac Hi s cag G n gca Al a ttt Phe gca Al a 175 acc Thr tct Ser at t SIle gaa Gu gca Al a 255 cgt Ar g at t SIle ggt G y gag Gu ttg Leu gt c Val 160 acc Thr agc Ser at t SIle tgc Cys gaa Gu 240 gt a Val cgc Ar g t cc Ser gtt Val ct c Leu ggc Gy 145 ct a Leu tcg Ser gac Asp at g Met t ttt Phe 225 cgc Ar g t ca Ser gga G y ttg Leu gga G y at t SIle 130 act Thr gga Gy gt c Val cct Pr o aac Asn 210 gct Al a gat Asp at g let gac Asp acg Thr 290 gct Al a gcg Al a gtt Val ttt Phe tct Ser 180 cct Pr o aag Lys tct Ser att SIe gtg Val 260 gtc Val ttg Leu 1006 <210> 184 <211> 302 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 184 Val Ser Val Ala G u G u y Lys Leu Phe Thr Pro Thr Phe Val let 1 5 10 Sy Trp Phe Ala Asn Leu Phe G n Phe Leu Val Phe Tyr Phe Leu Ile 25 Page 207 s Thr Thr IVet Ala Leu Tyr Ala li e 40 eql i st cor r ect ed. t xt Lys G u Phe G n Al 4 a Ser G u Val G u Ar g Val Pr o Val Leu Gy 145 Leu Ser Asp let Phe 225 Ar g Ser Gy Leu Gy Phe SIle G u Tyr 115 Pr o Thr Thr SIle G u 195 Pr o Tyr Leu Phe Asn 275 I le Al a Gy Val Leu Phe Ser Ser Asp 165 Leu Ser SIle Gy Thr 245 Met Val Ser Ser Ty r 70 Leu Pr o Al a Ar g Al a 150 Ty r SIle Gy I e Val 230 Gy Ar g SIle Phe Ser I le Val Leu Al a Ar g 135 Al a Asp Al a G u Pr o 215 I le Al a Ser Tyr Al a 295 Ser SIle Thr Leu Thr 120 Ser Leu let Val Pr o 200 I le Al a Gy Phe Phe 280 Thr SIle Asp Thr Tyr 105 Al a G u Gy Leu Val 185 Al a Gy Tyr Leu Leu 265 Gy Ser SIle Phe Al a Asn let Thr Al a 155 I le let Phe Phe Asn 235 Phe Lys Phe Tr p Gy Gy Cys Ar g Al a Gy 140 Leu Val Tyr Ser SIle 220 Al a SIle Leu Phe Hi s 300 Al a Ar g Al a Phe let 125 Tyr Al a Val Phe Phe 205 Leu Phe Al a G n Phe 285 Val Val Ar g Leu Leu 110 Val Leu Leu Leu Lys 190 Lys Leu Al a Tyr Asp 270 Val Val Phe Lys Tyr Hi s G n Al a Phe Al a 175 Thr Ser SIe G u Al a 255 Ar g SIe <210> 185 <211> 568 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(568) <223> RXA00858 <400> 185 ttttgttttt cagatgcatg ttagatgcgt tgagggacaa gggtggggga gacctccggt tcttaaattg tctaaccaag aaccggaggt tctttttgtc atg gaa gta aac tta 115 Iet G u Val Asn Leu 1 Page 208 seql i st cor r ect ed. t xt gcc aca tgg cta atc act atc gca gtg att Ala Thr Trp Leu Ile Thr Ile Ala Val lile 15 gat ttc tat tcc cac gtc cgc acc cca cac Asp Phe Tyr Ser His Val Arg Thr Pro His tcc gca tgg tgg agc ctc ttc tac gta gcc Ser Ala Trp Trp Ser Leu Phe Tyr Val Ala 45 gtg ttc ctc tgg ttt gct tgg ggc gag cca Val Phe Leu Trp Phe Ala Trp y G u Pro 60 ggc att gag ttc ttc acc ggt tac gtg aca Sy lie G u Phe Phe Thr y Tyr Val Thr 75 gat aac ctc ttc atc ttc gcg ctg atc atg Asp Asn Leu Phe Ile Phe Ala Leu Ile IVet 95 cgc aag tac cag cag aag gtt ctg ctc atc Arg Lys Tyr n G n Lys Val Leu Leu Ile 105 110 gtc ttc cgc ctg gca ttc atc ctc gca ggt Val Phe Arg Leu Ala Phe Ile Leu Ala y 120 125 tgg tcc gat gtc ttc tac atc ttc tcc atc Trp Ser Asp Val Phe Tyr Ile Phe Ser Ile 135 140 gtg aag gct cct gtg cac gag Val Lys Ala Pro Val His G u 150 155 <210> 186 <211> 156 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 186 Iet G u Val Asn Leu Ala Thr Trp Leu Ile 1 5 10 G y Phe Phe lie Phe Asp Phe Tyr Ser Hi s Pro Thr Ile Lys u Ser Ala Trp Trp Ser 40 Ala Cys Val Phe y Val Phe Leu Trp Phe 55 Asn Pro His On His G y lie G u Phe Phe 70 Lys Ala Leu Ser Val Asp Asn Leu Phe Ile 90 Ser Phe Lys lie Pro Arg Lys Tyr G n G n 100 105 Pag gct ggc ttc Al a G y Phe ttc att ttc gag G u ct c Leu ggt G y gag G u 80 ggt G y ggt Gy gct Al a tgg Tr p ccc Pr o gcc Al a aac Asn aag Lys tct Ser at c SIle gca Al a ct g Leu 145 I le Ar g Phe Tr p Gy Al a Val act Thr tgt Cys cca Pr o gcg Al a ttc Phe gcg Al a gtt Val 130 at c SIle Al a Thr Tyr Gy Tyr Leu Leu I e aaa Lys ttc Phe cag G n agt Ser at t SIle 100 gca Al a gaa G u acc Thr l Ie Hi s Al a Pr o Thr bet I e Phe gaa G u ggc G y cac Hi s gtt Val cct Pr o ctg Leu gcc Al a gct Al a Al a G u Leu Sy G u Sy Sy Thr Val Leu Al a Thr 75 Phe Lys e 209 seqI i st car r ect ed. t xt lieAla Leu Ala Leu Val Phe Arg Leu Ala Phe Ilie Leu Ala(3Gy Ala 115 120 125 Al a Val I Ie (31u Al a Tr p Ser As p Val Phe Tyr Ilie Phe Ser I Ie Tr p 130 135 140 Leu Ilie Tyr Thr Al a Val Lys Al a Pro Val Hi s (31u 145 150 155 <210> 187 <211> 975 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221> CIDS <222> (952) <223> RXA02305 <400> 187 t at gcgcgca ggt gt ctact ggt gacgcag ccgacgacga t tatt t tgac gaagccaccg caaacgat ga ct tcgat ccc gaaaagt gga ggaacat gt a gca Al a gca Al a g aa (31u gca Al a at t I Ie gca Al a gga G31y at t I Ie ac a Thr at c I Ie 1 50 cca Pr o t ct Ser aac As n ct g Le u t ac Tyr ct g Le u at g vb t cca Pr o 120 gt g ValI tt c Ph e at g vb t c ac Hi s ggc G31y g at As p ct c Le u gga G31y ct a Le u ag c Ser cat Hi s ggt (31y ccg Pr o t t c Ph e t ac Tyr cag O31n t cc Ser 75 cga Ar g gct Al a gag G31u gaa G31u gag G31u 155 t at Tyr t ac Tyr cgc Ar g cgc Ar g t ac Tyr at t I Ie gt t ValI g aa G31u ct c Le u 140 ct g Le u t gg Tr p g aa G31u at g vb t 45 ggt G31y g at As p ct g Le u g at As p t ca Ser 125 ac c Thr tt c Ph e at c I Ie aac As n gct Al a g aa G31u ct g Le u gcc Al a act Thr 110 tt c Ph e act Thr act Thr ct g Le u ct c Le u ct g Le u ag c Ser gcc Al a cca Pr o G31y gct Al a t cc Ser ac a Thr 1 at g vb t 1 t cc Ser ggc G31y c ag O31n at c lIe act Thr act Thr gca Al a gct Al a aaa Lys5 145 ac c Thr cca gcc ttt Pro Al a Ph e ac c Thr t gg Tr p gga G31y ccg Pr a gca Al a g ac As p ct g Le u ggt G31y 130 t at Tyr t ct Ser t cg Ser g aa O31u ct a Le u g at As p aag Lys5 gt g ValI tt c Ph e 115 g aa O31u t cc Ser g aa (31u g ac As p at t I Ie ccc Pr a ac c Thr aag c ac Hi s 1 00 ggc G31y cca Pr a g aa O31u at g vb t Page 210 agt Ser t cc Ser gt t ValI gct Al a t gg Tr p 230 gcc Al a g aa G u tt c Ph e g aa (31u tt t Ph e ag c Ser 200 gcg Al a t ca Ser at t I Ie g at As p aag Ly s 280 g at As p 1 70 gga G31y t gg Tr p gcc Al a tt t Ph e tt g Le u 250 ct c Le u g aa a u ag t Ser at t I e cag O31n aag Ly s ggc G31y 235 t gt Cy s t t c Ph e gga (31y tt c Ph e tt t Ph e t cc Ser g aa (31u 220 cgc Ar g g aa O31u g ac As p c ag O n seql i st car r ect ed. t xt cgc tac acc acc gca ttg gct Ar g Tyr Thr Thr Ala Leu Al a 1 75 gat gcc aaa ggc cac ttc cca Asp Al a Lys G31y Hi s Phe Pr o 190 195 tac ct t ggc gt g ct c aac gcc Tyr Leu G31y Val Leu Asn Al a 205 210 t tt ggt ggc gat gt t at t cgt Phe G31y G31y Asp Val Ilie Ar g 225 at g gt t ct c at c t ct gat t cc I'b t Val Leu Ilie Ser Asp Ser 240 gt a gag gaa t ac gt c gag gaa Val O31u O31u Tyr Val O31u O31u 255 at c gat ct c agt gct t tc gaa Il e Asp Leu Ser Al a Phe (3 u 270 275 t aat cct aca gcgccat gga gga g ac As p 1 cct Pr o g at As p aag Ly s act Th r gca Al a 260 gag G u <210O> 188 <21 1> 284 <21 2> PRT <21 3> Cor ynebact er i urn <400> 188 I'bt Pro Al a Phe O3u Al 1 5 Ser Thr Ser Asp I I e Al G31y Tr p O31u IIe (31u (3 O31n G31y Leu Pr o Val Al li1e Pr o As p Thr Tr p 1 1 Thr Al a Lys Lys I I e Al Thr As p Val Hi S Leu (3 1 00 Al a Leu Phe G31y Val 1 1 115 Ala (31y O31u Pr o G3y T[ 130 Lys Ty r Ser 03 u Al a 1 1 145 1 gi ut ani cumn a I'bt Pro G1y I'bt Pro Tyr Trp Ilie a U a e 0O a y e e 30 Ly s ValI G31y Thr (31u Ar g (31u Ser 135 As p Al a 25 As p I Ie Ph e G31y I Ie 1 05 G31y Tr p Tyr 10 Hi s Phe O3 y Ty r As p O31n Leu Ser- 75 G31y Ar gI 90 Leu Al a Ser 03 u Hi s 03 u I (31y 1ul 1 55 Page 211 O31u vb t G31y As p Le u As p Ser 125 Thr Ph e As n Al a O31u Le u Al a Thr 110 Ph e Thr Thr Asp Leu Val Leu Arg Leu Ser Ser As p Al a O31u Pr o Al a 03 y Val Al a Val Ser Tr p Thr 1 Thr A a Hi s Le u ValI 225 Ser ValI A a O31u A a Pr o 1 95 A a Ar g Ser O31u O31u 275 A a 1 65 O31y O31n As p Pr o G31y 245 A a O31n Ser Ser ValI A a Tr p 230 A a O31u Ph e A a A a Pr o A a 215 As p Thr G31y Ar g seql i st car r ect ed. t xt O31u As p As p Se r Ph e Ar g 1 70 Phe A a 03 y I I e Phe As p 1 85 Ser Phe Tr p O31n Ser Ty r 200 205 A a Lys A a Lys 03 u Phe 220 Ser O31u Phe O31y Ar g [Vb t 235 li1e Thr Leu Cys O31u Val 250 As p As p Leu Phe As p I I e 265 Lys 03 n 03 u 03 y 03 n 280 Ty r A a 1 Le u O31y ValI O31u As p 270 Th r 1 Ly s O31y O31y Le u O31u 255 Le u <210O> 189 <21 1> 948 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (925) <223> RXA00084 <400> 189 t caccct t gt cgat accagc t act gggt at ct ggcgt cgg t ct tggaaga cat cgat gcc t tcct cgacg cacagcaat a tccacttggc ggcagcaaag t cc aca gct ct c Ser Thr A a Leu ccc Pr o c ag O31n g ac As p ct g Le u g ac As p tt t Ph e c ag O31n c aa O31n ct c Le u at t I Ie ccc Pr a gcg A a aag Ly s ct t Le u tt c Ph e ag c Ser ccc Pr a c aa O31n t gg Tr p ag t Ser tt t Ph e ccg Pr a cca Pr a 75 cga Ar g g aa O31u ccc Pr a O31y gct A a ccg Pr a ccg Pr a agt Ser tt c Ph e t gg Tr p aac As n ac g Thr tt g Le u gcc A a 15 at g vb t ggt O31y at c I Ie t gg Tr p cgc Ar g 95 tt c Ph e cgg Ar g tt g Le u cca Pr a ct t Le u ac c Thr gt g ValI ac c Thr c aa O31n tt c Ph e cgt Ar g ac c Thr c ag O31n gt c ValI cgc Ar g gga O31y gct A a cca Pr a at c lIe act Thr at c I Ie at t I Ie aag Ly s t ct Se r 1 00 gt c cga ccc gaa ct a t gc gaa gt a gac at t gat at c t at ct t cac aac Page 212 Val Arg Pro u Leu Cys u 105 seql i st cor r ect ed. t xt Val Asp Ile Asp Ile Tyr 110 Leu His Asn 115 cct Pr o gaa G u gga G y 150 ggc G y aaa Lys gt a Val gct Al a cct Pr o 230 at t l Ie act gga G y 120 at c SIle acc Thr tgt Cys cct Pr o at c SIle 200 aaa Lys gt a Val aaa Lys ct c gt a Val acc Thr cat Hi s t ca Ser 170 acg Thr gat Asp aag Lys ttc Phe ct a Leu 250 t ac Tyr aga Ar g cct Pr o 140 act Thr ccc Pr o ttc Phe t cc Ser ttc Phe 220 at c SIle aac Asn aaa Lys tgg Tr p 125 gac Asp tct Ser gcg Al a ct c Leu acc Thr 205 caa G n gcc Al a agc Ser t ac Tyr gca Al a gt a Val gcg Al a at c l Ie cac Hi s 190 aag Lys tgg Tr p ggt G y t ac Tyr ggc G y 270 aag Lys cgc Ar g at c l Ie gca Al a 175 gt a Val ct c Leu gct Al a gaa G u cga Ar g 255 cga Ar g aac Asn gca Al a gat Asp 160 aat Asn gac Asp act Thr t ca Ser act Thr 240 gtt Val cga Ar g agt Ser 130 gaa G u ct c Leu gt c Val ct a Leu gaa G u 210 aat Asn at g Met t cc Ser gt a Val gtt Val acc Thr tgt Cys aat Asn gaa G u 195 gac Asp gca Al a gt g Val t ca Ser gac Asp 275 gac Asp ggc Gy ct c Leu caa G n 180 gat Asp at c SIe gca Al a cgc Ar g cga Ar g 260 Thr Phe Leu tagctttcag attcagaccc cag <210> 190 <211> 275 <212> PRT <213> Corynebacter i um gl utam cum <400> 190 IVet Ser Thr Ala Leu Pro Asp G n Leu 1 5 Pro Val n Ile Ser n Lys n Arg I e Thr Val Thr y Asp Lys Leu Arg 40 Asp n Arg Ile Lys Leu Ile Ile Pro 55 Asp Phe y Ile Leu Asp u Pro Thr 70 Pro Arg Ala Lys Ser Phe Pro Ala Asp Lys Trp G u 10 Leu Ser Pro Phe Phe G y Ser Pro Al a Pro Pro Pro G n Arg Pro Page 213 Tyr Sy G n Sy Thr SIle Al a bet Gy I e Tr p Ar g seql i st cor r ect ed. t xt Ala Val Arg Pro Leu Cys G u Val Tyr SIle Cys Gy 145 Ar g SIle Ser Phe Al a 225 Ser Asp Thr Pr o Leu 115 Val Thr Cys Asn G u 195 Asp Al a Val Ser Asp 275 Asn Asp Tyr SIle 165 Ser Asp Asp Asp Phe 245 I le Ser Leu 135 I le Asp Val Leu Tyr 215 Ser Ar g Phe G u 105 Pr o SIle Tyr Al a Thr 185 Al a Al a Hi s G u Gy 265 Ser Gy Pr o 155 Al a Val Ser Val Tr p 235 I le Tr p Tr p 125 Asp Ser Al a Leu Thr 205 G n Al a Ser Tyr Asp 110 Al a Val Al a l Ie Hi s 190 Lys Tr p Gy Tyr Gy 270 SIle Lys Ar g l Ie Al a 175 Val Leu Al a G u Ar g 255 Ar g Asp Asn Al a Asp 160 Asn Asp Thr Ser Thr 240 Val Ar g <210> 191 <211> 468 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(445) <223> RXA00843 <400> 191 gccctgatgc gaaaccggcg ccaacaatga tgccgacgaa tttgaataat catggaacaa accttagtag gctcaacgtt ttc cat aat Phe Hi s Asn cgc gac aag Arg Asp Lys ccc acc gct Pro Thr Al a ccg cgt tgt tcc aca tcc Pro Arg Cys Ser Thr Ser gac att gag cct gaa att Asp lie u Pro Gu lie 30 t cc gag ctc aaa gaa ct a Ser G u Leu Lys G u Leu 45 aga aat Arg Asn 15 gtt cag Val G n ttc aat Phe Asn Page 214 ggcaaatgcc actcttagga atg aaa gtc acg att IVet Lys Val Thr Ile 1 acc ctc gct tac ctc Thr Leu Ala Tyr Leu tat ctc aaa gac acg Tyr Leu Lys Asp Thr acg ctg gga att cca Thr Leu Gy lie Pro gtc cac gac ggc atc aga a Val His Asp Gy lie Arg T\ ctg tca cca gaa aca cct g Leu Ser Pro G u Thr Pro G 75 cat ccc agg ctc ctt cag c! His Pro Arg Leu Leu G n Ar cgc att gcg cgc ccc aaa at Arg Ile Ala Arg Pro Lys II 105 tgacaacatt ttgtagagca acc <210> 192 <211> 115 <212> PRT <213> Corynebacteri um gl ut <400> 192 Mbt Lys Val Thr Ile Phe Hi 1 5 Thr Leu Ala Tyr Leu Arg A Tyr Leu Lys Asp Thr Pro Tt Thr Leu Gy lie Pro Val Hi Tyr Thr G u Leu G y Leu S 70 Asp Ala lle Val Ala His Pr Thr Ala Lys Gy Ala Arg II 100 Ser Ile Leu 115 cc hr 60 aa u gt rg :c e seql i st cor r ect ed. t xt cgc gaa gct gag tac aca Arg G u Ala G u Tyr Thr act gag ctt atc gac gcc Thr u Leu Ile Asp Al a 80 ccg atc gtg gtg acg gcc Pro Ile Val Val Thr Ala 95 gac gtc att gac agc atc Asp Val lie Asp Ser lle 110 gaa G u at c l Ie aaa Lys ttg Leu 115 ctg ggc Leu G y gtt gcc Val Ala ggc gcg G y Al a 100 arm cum s Asn Pro sp Lys Asp hr Al a Ser 40 s Asp y er Pro u o Arg Leu e Al a Ar g 105 Cys G u Leu Ar g Pr o G n Lys Thr G u G u Ar g Thr Pr o Asp Ar g Val Phe Al a Leu Val I e <210> 193 <211> 432 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(409) <223> RXA01052 <400> 193 tatggccaac cctaggggga tggcctgtgt gttcactgtt aggtttcctc aaaatcttt acgaacaacg aagagcttgc ccgagagtat cttgggtcgc atg gac aca aaa tta IVet Asp Thr Lys Leu 1 ggc gct gaa ttg ggt act gaa ttt gat ctc att gtt gtt ggt ttc ggc Page 215 a 115 163 INO seql i st cor r ect ed. t xt SQy Ala Qu Leu Gy Thr Gu Phe Asp Leu lie Val Val y Phe y 15 aaa gca ggc aag act atc gcg atg aaa cgc tcg gca gcg ggg gat aag 211 Lys Ala G y Lys Thr Ile Ala IVet Lys Arg Ser Ala Ala G y Asp Lys 30 gtc gca ctg atc gag cag agt cca cag atg tat ggc ggt acc tgc atc 259 Val Ala Leu Ile u n Ser Pro n Vet Tyr y y Thr Cys Ile 45 aat gta ggt tgc atc ccc acg aag aag ttg ttg ttt gag act gca acg 307 Asn Val y Cys lie Pro Thr Lys Lys Leu Leu Phe G u Thr Ala Thr 60 00 0 ggc aag gat ttc ccg gat gcg gtt gtg gcg cgt gat cag ttg att ggc 355 0 G y Lys Asp Phe Pro Asp Ala Val Val Ala Arg Asp n Leu Ile y CK1 70 75 80 NO 0 aag ctg aat gcc aag aat ctt gcg atg gcc aca gac aag ggt gtc acc 403 0 Lys Leu Asn Al a Lys Asn Leu Al a IVet Al a Thr Asp Lys G y Val Thr CK1 90 95 100 cgt cat tgatggaaaa gctacgttta cag 432 Arg His <210> 194 <211> 103 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 194 IVet Asp Thr Lys Leu G y Al a u Leu G y Thr G u Phe Asp Leu I e 1 5 10 Val Val G y Phe G y Lys Al a G y Lys Thr I e Al a Iet Lys Arg Ser 25 Ala Ala y Asp Lys Val Ala Leu I e u n Ser Pro n IVet Tyr 40 Gy y Thr Cys Ile Asn Val y Cys lie Pro Thr Lys Lys Leu Leu 55 Phe G u Thr Ala Thr G y Lys Asp Phe Pro Asp Ala Val Val Ala Arg 70 75 Asp G n Leu I e G y Lys Leu Asn Al a Lys Asn Leu Al a IVet Al a Thr 90 Asp Lys G y Val Thr Arg His 100 <210> 195 <211> 543 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(520) <223> RXA01053 <400> 195 ttgcgatggc cacagacaag ggtgtcaccc gtcattgatg gaaaagctac gtttacagct Page 216 seql i st cor r ect ed. t xt agccacgaaa tcacaagtaa ct t caggtag tgacactct t acg att gtg atc aac acg ggc tcc acg Thr l e Val lie Asn Thr Gy Ser Thr ggc acc gac aat ccg cat gtt ttt gat Gy Thr Asp Asn Pro His Val Phe Asp 30 tcg ccc ctg ccg aag cac ctc gcg atc Ser Pro Leu Pro Lys His Leu Ala lie 45 ttg gaa ttt gcc acg ctt ttc agt gga Leu G u Phe Ala Thr Leu Phe Ser G y 60 atc gac cgt ggt gaa ttg ccg ctg aaa le Asp Arg G y G u Leu Pro Leu Lys 75 gag ctg gcc aaa acc gac ctg gag gcc G u Leu Ala Lys Thr Asp Leu G u Al a aac gct gaa ctc acc gga ttc agc ggt Asn Al a G u Leu Thr G y Phe Ser G y 105 110 gac cac gac ctc ctc gcc gac gcc gca Asp His Asp Leu Leu Ala Asp Ala Ala 120 125 cgg cac cga cgg gct cgg cct tgaacag! Arg His Arg Arg Ala Arg Pro 135 140 <210> 196 <211> 140 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 196 Val Leu Tyr Ala Pro Thr Ile Val lle 1 5 lie Pro Asn Val Pro G y Thr Asp Asn 25 G y lie G n His lie Ser Pro Leu Pro 40 Sy y Pro Ile y Leu u Phe Al a 55 Ser Lys Val Thr Ile I e Asp Arg y 70 Asp Arg G u Val Ala G u Leu Ala Lys Ile Thr Phe Leu Asn Asn Ala G u Leu 100 105 1 ccg gt c at c Pro Val li e 15 t cc act ggc Ser Thr G y at c ggc ggt lie G y G y caa ggc t cc Sn G y Ser aat ttc gac Asn Phe Asp cgc gga at c Arg y lle 95 gac ctc acc Asp Leu Thr ctt ttt gca Leu Phe Al a ct g t at Leu Tyr ccc aat Pro Asn att cag Ile G n ggc ccc G y Pro aaa gt c Lys Val agg gaa Arg u acc ttc Thr Phe atc gcg I e Al a 115 tcg gcc Ser Al a gcg Al a gtc Val cac Hi s at c SIe acc Thr gta Val ct c Leu 100 ct c Leu gac Asp gcg ggcatcaaaa cag Asn Thr G y Ser TI 10 Pro His Val Phe A Lys His Leu Ala II Thr Leu Phe Ser G G u Leu Pro Leu L Thr Asp Leu G u Al 90 Thr G y Phe Ser G 1 Page 217 hr sp e y ys a y Pr o Ser SIe G n Asn Ar g Asp seqI i st car r ect ed. t xt Thr I Ie Al a Leu Lys Asp Hi s Asp Leu Leu Al a Asp Al a Al a Leu Phe 115 120 125 Al a Se r Al a Asp Al aArg His Ar g Arg Al aArg Pro 130 135 140 <210O> 197 <21 1> 612 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (589) <223> RXA01054 <400> 197 gacctcctcg ccgacgccgc actttttgca tcggccgacg cccggcaccg acgggctcgg ccttgaacag gcgggcatca aaacaggcac gcgtggggag gt g ct t gt c gac gcc Val Leu Val Asp Al a c ac Hi s ggc G31y ct g Le u at c I Ie aac As n at g vb t gt g ValI tt g Le u at g vb t c ac Hi s 1 50 cgg Ar g ccg Pr a c aa O31n ac c Thr g aa (31u at c I Ie tt t Ph e t gc cys 120 cat Hi s gcc Al a acc aac at c Thr Asn Il e cag t tt acc 03 n Phe Thr cta gcc gga Leu Al a 03 y acc acg ttc Thr Thr Phe ggg gaa aat (31y 1u Asn 75 gtt ccc cga Val Pr o Ar g ttc gtc gac Phe Val Asp 1 05 gcc gac tcc Al a Asp Ser ggc gtc acc G31y Val Thr acc t cg gag Thr Ser 03 u 155 g ac As p t ac Tyr ac a Thr at c I Ie gt g ValI cca Pr a aag Lys5 c ag O31n gcc Al a 140 at c I Ie at c I Ie t cc Ser 30 aag Lys5 ccg Pr a gt g ValI at t I Ie t ct Ser 110 ct c Le u gag O31u aac As n tt c Ph e 15 t ac Tyr aaa Lys5 ccg Pr a aaa Lys5 at t I Ie 95 g at As p at c I Ie ct t Le u c aa O n gct Al a g at As p t cc Ser tt a Le u aag Lys5 aac As n gcg Al a aac As n ggc G31y tt a Le u 1 1 gt a ValI g ac As p at t I Ie t cc Ser gcc Al a c aa O31n ct g Le u ac c Thr g ac As p 145 tt g Le u ggt O31y c ac Hi s gca Al a ac c Thr tt g Le u cct Pr a ct c Le u gt g ValI 130 ggc O31y ggc (31y g at As p cgc Ar g c ac Hi s at c I Ie at t I Ie c ac Hi s ggc O31y 115 gcg Al a at c I Ie agt Ser gt c ValI at t I Ie cga Ar g ggt O31y gca Al a ggt O31y 1 00 gcg Al a ct t Le u t ac Ty r taacgcagcg gatcgaacgg ctt Page 218 seqI i st car r ect ed. t xt <210O> 198 <21 1> 163 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 198 Val Leu Val Asp Ala Hi s Leu Ar g Thr Val As p 00 Sle \Q Ser S 65 A~K Aa O31n Le u Thr As p 145 Le u As p Ar g Hi s I Ie I Ie Hi s O31y 115 A a I Ie Ser As n ValI Le u As p As p vb t Thr A a Thr O31y As p Pr a Thr Pr a Lys5 Tyr Ar g 135 Pr a O31n Le u Thr G31y ValI Ph e 1 A a G31y Thr As n 10 Ph e A a Thr O31u Pr a 90 ValI As p ValI Ser I Ie Thr G31y Ph e As n 75 Ar g As p Ser Thr O31u 1 55 As p Tyr Thr I Ie ValI Pr a Lys5 O31n A a 140 li1e G31y ValI G31y (31u ValI (31u O31n O31u 125 Ser Ph e Ph e Tyr Lys5 Pr a Lys5 I Ie As p I Ie Le u O31n A a As p Ser Le u As n A a As n O31y Le u 1 <210O> 199 <21 1> 561 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (538) <223> RXN03123 <400> 199 agctct acca acgcgcct ac acct tgacca acgt ggat gc tggcttttgt gctgcacgag ccgctggggc ccgcctcggc cga ggc cgg Ar g O31y Ar g gcc at c cca Ala Ilie Pr o acc t ct t ac Thr Ser Tyr gga aag cct gaa gtc atg O31y Lys Pro O31u Val I'b t gat cca gcg ccg cgt ggc Asp Pr o Ala Pr o Ar g O31y 30 cca gcg at c t gc t cg at t Pr o Ala I Ie Cys Ser Ilie 45 cgc t ac Ar g Ty r 15 t tc ct t Phe Leu ct g gag Leu 03 u Page 21~ cgat gccggt acct t t gacc gt g ggc gac gcg ct g Val O31y Asp Ala Leu 1 cca gga att ccg tt c Pro 31y Ilie Pr o Phe t tc t ta ggc gat ct c Phe Leu 03 y Asp Leu acc t tg gac ggt gaa Thr Leu Asp G31y (31u atc cct gcg acc Ile Pro Ala Thr ttc gat ttt ccc Phe Asp Phe Pro gaa caa tcc ttc G u G n Ser Phe tat acc tgg atc Tyr Thr Trp Ile 105 cat ctg cag acc His Leu G n Thr 120 ggt tat tgg aac Gy Tyr Trp Asn 135 ttgcttatcg acg <210> 200 <211> 146 <212> PRT <213> Corynebact <400> 200 Val Gy Asp Ala 1 Pro Gy lie Pro Phe Leu G y Asp Thr Leu Asp y Pro Leu Asp Tyr Sn Trp lie Ser Asp Tyr Thr Asp 100 Arg Ala Ala Lys 115 His IVet Asn Al a 130 Ser Asn 145 <210> 201 <211> 736 <212> DNA gcg Al a cag G n at t SIle ggc G y cac Hi s aag Lys t at Ty r ggc G y 75 gat Asp gcg Al a gcc Al a ggc G y ctt Leu 60 gag G u cac Hi s gaa G u ggc G y aga Ar g 140 seql i st cor r ect ed. t xt atc gcc cac gat cca ctt lie Ala His Asp Pro Leu cac atc acc gcg cag tgg His Ile Thr Ala n Trp 80 atc gct gac acg gat tac Ile Ala Asp Thr Asp Tyr 95 tcc tcc gaa acc cgt gcg Ser Ser G u Thr Arg Ala 110 atg ccc aag acg cac atg bet Pro Lys Thr His bet 125 130 gcc atg ggt aaa agc aat Ala IVet y Lys Ser Asn 145 gat t ac acc Asp Tyr Thr att tcc aac Ile Ser Asn acc gat ttt Thr Asp Phe 100 gcc aag aag Ala Lys Lys 115 aac gcg caa Asn Al a G n t aaaagat t t er i um gl ut am cum Leu Arg G y Arg G y 5 Phe Ala lie Pro Asp 25 Leu Thr Ser Tyr Pro 40 G u Il e Pro Ala Thr Thr Phe Asp Phe Pro 70 Asn G u G n Ser Phe Phe Tyr Thr Trp lle 105 Lys His Leu G n Thr 120 Sn y Tyr Trp Asn 135 Lys 10 Pr o Al a Al a G n SIle 90 Sy Hi s Lys Pr o Al a l Ie Tyr Sy Asp Al a Al a Sy Val Ar g Ser I le Hi s SIle Ser bet 125 Al a vet Sy I le Al a SIle Al a Ser 110 Pr o bet Ar g Phe Leu Hi s Thr Asp G u Lys Sy Page 220 seqi i st car r ect ed. t xt <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (736) <223> FRXA00993 <400> 201 gct gagct ag t gctt t tgcg acacacct ct t gcgaat gt t gat taggt ta ggcaagccat atttacaggg tgttgtgaaa gcataaggga gcaaggaaac at g ggc aag gga tt t l'bt G31y Lys G31y Phe ac c Thr ac c Thr cat Hi s ct g Le u cgc Ar g ct g Le u ac g Thr at t G31y g ac As p 1 50 g at As p at t I Ie ac c Thr gct A a gga G31y gag O31u ct g Le u t ac Tyr tt t Ph e t gc cys tt c Ph e 120 ct c Le u g aa O31u ac c Thr aac As n tt t Ph e 200 tt g Le u ac c Thr ct g Le u tt c Ph e tt g Le u ct g Le u gcc A a at c I Ie t ct Ser cct Pr a g at As p 1 70 c aa O31n ac c Thr gt c ValI at c I Ie aac As n c ac Hi s aac As n gag (31u g aa (31u g at As p cca Pr a 140 ac c Thr ccc Pr a tt c Ph e at c I Ie at g vb t aat As n ac g Thr 45 gag (31u gt g ValI ccg Pr a ag c Ser cca Pr a 125 gcg A a gcg A a c ag O31n at t I Ie ggc G31y 205 ggc G31y g ac As p g aa O31u ag c Ser g at As p ct g Le u ct g Le u 110 gcg A a at c I Ie t at Tyr G31y g at As p 1 90 gcg A a gt g ValI 15 cgc Ar g ggt G31y cga Ar g gcc A a ggg G31y 95 g aa O31u ccg Pr a t gc cys ct t Le u gag O31u 1 75 c ac Hi s g aa O31u aaa Ly s at g vb t g aa O31u cct Pr a g at As p 80 ccc Pr a gt c ValI cgt Ar g t cg Ser at c I Ie 1 60 c ac Hi s at c I Ie t cc Ser 1 t cg Ser gt g ValI gt c ValI gga G31y 65 gcc A a gcc A a at g G31y at t I Ie 145 gcc A a at c I Ie gct A a t cc Ser cat Hi s ac c Thr ccc Pr a 50 aag Ly s ggt G31y t cg Ser cgc Ar g tt c Ph e 130 ct g Le u c ac Hi s ac c Thr g ac As p g aa O31u 210 at c I Ie at t lIe 35 ggc G31y ct c Le u ac c Thr gcg A a t ac Tyr 115 ct t Le u gag O31u g at As p gcg A a ac g Thr 1 ac c Thr gcc A a 20 cat Hi s g at As p t ac Tyr tt t Ph e t gg Tr p 1 00 cca Pr a tt c Ph e ac c Thr cca Pr a c ag O31n 1 g at As p cgt Ar g ac c Thr tt t Ph e t gg Tr p c aa O31n g ac As p gcg A a gga O31y ct a Le u tt g Le u ct t Le u 1 t gg Tr p t ac Ty r <210O> 202 Page 221 seqI i st car r ect ed. t xt <21 1> 212 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 202 N/t G31y Lys 0 y Phe Thr 0 y Al a Il e Ser ValI ValI G31y Al a Al a vb t G31y I Ie 145 Al a I Ie Al a Ser lIe lIe G31y Le u Thr Al a Tyr 115 Le u O31u As p Al a Thr 1 95 Thr Thr Ph e Tr p O31n As p Al a G31y Le u Le u Le u 1 65 Tr p Tyr Thr Ser Ar g Al a Al a Ar g Pr a As p 135 G31y Tyr Ser As p Ly s Thr Tr p Thr ValI G31u 1 05 Al a Thr I Ie Ph e G31u 1 85 Tyr Thr ValI Le u Pr a Thr Hi s G31y Pr a Tyr Al a 1 Ph e Ser Tr p As n Thr G31u ValI Pr a Ser Pr a 125 Al a Al a O31n I Ie G31y 205 G31y As p O31u Ser As p Le u Le u 110 Al a I Ie Tyr G31y As p 1 Al a ValI Ar g G31y Ar g Al a G31y O31u Pr a cys Le u O31u 1 Hi s (31u <210O> 203 <21 1> 732 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (709) <223> RXA01051 <400> 203 t gcgacccaa gat act ct cg ggcaagct ct t cgt tgt tcg t taaagat tt t gaggaaacc taacagtgaa cacacaggcc atccccctag ggttggccat at g t ca acc at t cac 115 I'b t Ser Thr I Ie Hi s 1 gcc t cc gga at c cag gct cca caa gt g cca cac ggt t cc cac cat gcc 163 AlaSer cOy Ile(3nAla ProOnVal Pro His (3y Ser His Hi sAl a 15 Page 222 seql i st cor r ect ed. t xt ccg cca caa aag gac gaa tca gtg aag aag agc ttc aat gcc tct tct 211 Pro Pro G n Lys Asp u Ser Val Lys Lys Ser Phe Asn Ala Ser Ser 30 tta ctg ttc gcg ttt tcc ttc ggc gtg tac ctg gtg ctg ctt gtg atg 259 L Leu Leu Phe Ala Phe Ser Phe G y Val Tyr Leu Val Leu Leu Val Iet 45 atg aca ctt ctt aaa agt cgc ctt tct tta ggc gga ctg tgg aac aca 307 IVet Thr Leu Leu Lys Ser Arg Leu Ser Leu G y G y Leu Trp Asn Thr 60 0 gaa gca cac caa tac aga tcc atc gac tta gag ctt ttc aac ggc ttt 355 00 u Ala His G n Tyr Arg Ser Ile Asp Leu G u Leu Phe Asn y Phe S 70 75 80 CK1 gct gat cca cca att tgg tgg ggg cct tgg acc aac act ttt ggc aac 403 CO Ala Asp Pro Pro Ile Trp Trp Gy Pro Trp Thr Asn Thr Phe Gy Asn 95 100 CK1 atc gca ctg ttc atg cca ttt ggg ttt ttc ctg tac aaa atg ctc cgt 451 Ile Ala Leu Phe IVet Pro Phe G y Phe Phe Leu Tyr Lys IVt Leu Arg 105 110 115 aga ttc aac cat cga ttc ccc ttc gta gaa acc atc ctg ttt gcc agc 499 Arg Phe Asn His Arg Phe Pro Phe Val G u Thr Ile Leu Phe Ala Ser 120 125 130 gtc acc agc ctc agt atc gaa gtt ctg caa tgg gtg ttt gct att gga 547 Val Thr Ser Leu Ser Ile G u Val Leu G n Trp Val Phe Ala lie y 135 140 145 tat tca gat gtc gat gac ctg ttg ttt aat acg atc ggc gga ctc att 595 Tyr Ser Asp Val Asp Asp Leu Leu Phe Asn Thr lie Gy y Leu Ile 150 155 160 165 gga gca tcc gta gca gcg ctt gtc tcg ctt aaa tcc tcc aag gta gtc 643 G y Al a Ser Val Al a Al a Leu Val Ser Leu Lys Ser Ser Lys Val Val 170 175 180 agc gga atc atc atg ggc ggt tca cta tct gtg atg gcg atg atg atg 691 Ser Gy Ile Ile Iet G y y Ser Leu Ser Val IVet Ala IVet IVet IVet 185 190 195 tat tca agt ttt atc gcc tagaaggttt cagcagttcc gct 732 Tyr Ser Ser Phe lie Ala 200 <210> 204 <211> 203 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 204 IVet Ser Thr I e Hi s Ala Ser G y I e O n Al a Pro O n Val Pro His 1 5 10 G y Ser His His Ala Pro Pro G n Lys Asp G u Ser Val Lys Lys Ser 25 Phe Asn Al a Ser Ser Leu Leu Phe Ala Phe Ser Phe G y Val Tyr Leu 40 Val Leu Leu Val IVet IVet Thr Leu Leu Lys Ser Arg Leu Ser Leu G y 55 Page 223 Gy Leu Asn Tyr SIle Val 145 I le Ser vbt Tr p Asn Phe Met 115 Phe Al a Gy Lys Met 195 Thr Phe Asn Ar g Ser Gy SIle 165 Val Met G u 70 Al a SIle Ar g Val Tyr 150 Gy Ser Tyr Al a Asp Al a Phe Thr 135 Ser Al a Gy Ser seql i st cor r ect ed. t xt His n Tyr Arg Ser lie Pro Pro lie Trp Trp y 90 Leu Phe Met Pro Phe y 105 Asn His Arg Phe Pro Phe 120 125 Ser Leu Ser Ile G u Val 140 Asp Val Asp Asp Leu Leu 155 Ser Val Ala Ala Leu Val 170 Ile Ile e t Gy y Ser 185 Ser Phe Ile Ala 200 Leu Tr p Phe G u G n Asn Leu 175 Ser <210> 205 <211> 1359 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1336) <223> RXN01873 <400> 205 ccgtcgttgc ccatggtcac agcctacatg cac acattttact aatatttgct gttggcgcat gat gat Asp gag G u gcc Al a ccc Pr o acg Thr tcg Ser aag Lys gga G y ctt Leu at g Met tcg Ser ctt Leu gag G u cgc Ar g gct Al a gaa G u acg Thr gag G u gca Al a aat Asn tt c Phe gag G u act Thr 75 aaa Lys cac Hi s t ac Tyr aat Asn ttg Leu gga G y ttt Phe gaa G u aag Lys ggt G y 45 gga G y at g Met ggt Gy ggc G y cgc Ar g ctt Leu at t SIle ttg Leu cgc Ar g aaagtga atcaaaaaca gctatttcta gaactcc atg agc caa gca ata 115 Vet Ser Gn Ala lle 1 cac gaa ggc cac gaa ggc atc 163 His u y His u G y lie 15 gct gtg ttt gcg atg ctg gcc 211 Al a Val Phe Ala bet Leu Ala tat tgc acg cag gca ttg ctt 259 Tyr Cys Thr G n Ala Leu Leu acg ccc act gag tcc gcg ctg 307 To TPro Thr u Ser Ala Leu gcg ctg tgt att gtt ccg gcg 355 Ala Leu Cys Ile Val Pro Ala 80 ggt cgg gtg ctg aca att tca 403 G y Arg Val Leu Thr Ile Ser 95 100 Page 224 seql i st cor r ect ed. t xt ct c Leu at t SIle ggc G y aag Lys 150 ggc G y cat Hi s ggc Gy aag Lys cgg Ar g 230 ggt Gy cag G n ct g Leu at c SIle tcg Ser 310 ct g Leu gga G y at g M\et ttg Leu gct Al a 120 cca Pr o at t SIle ct c Leu caa G n at c SIle 200 at c SIle cct Pr o ttt Phe ggg Gy ggg G y 280 aat Asn gca Al a gt g Val at c SIle ttg Leu 360 atc atc gtg gga Ile Ile Val Gy at c l Ie gt g Val cat Hi s gga G y 170 gca Al a gt g Val ct g Leu ttg Leu tcg Ser 250 agt Ser tgg Tr p t ca Ser at g Met acc Thr 330 at c SIle tgt Cys ct g Leu gcg Al a gcg Al a 155 cgt Ar g ct g Leu gt g Val cgc Ar g gcg Al a 235 ct g Leu gaa G u agt Ser acg Thr ggg G y 315 gcg Al a at c SIle t at Tyr ct c Leu at g Met 140 at g Met at g Met ct g Leu ttg Leu cat Hi s 220 ttg Leu t ac Tyr gt g Val t cc Ser gtt Val 300 at t SIle gca Al a gca Al a t ac Tyr aga Ar g 125 acc Thr gga G y at t SI e gga G y ctt Leu 205 gag G u ctt Leu aac Asn ct g Leu acc Thr 285 at t SIle aat Asn ttt Phe acg Thr gt g Val 365 tta att ttg ccg ctt gtc Leu Ile Leu Pro Leu Val 110 115 ggt ctc caa ggt gcg ctg G y Leu n G y Ala Leu 130 tgg ttg tct gag gaa att Trp Leu Ser G u G u lie 145 att tac atc gcg gga aat Ile Tyr lie Ala Gy Asn 160 ccg gcg gga cta ctt gaa Pro Ala G y Leu Leu G u 175 agt tct atc gct gcg ctg Ser Ser Ile Ala Ala Leu 190 195 ccc aag cag cgg aaa ttc Pro Lys G n Arg Lys Phe 210 att tcg gcg atg gct gct Ile Ser Ala Vet Ala Ala 225 ttt ggt act gcg ttt ttg Phe G y Thr Al a Phe Leu 240 tat ttg ggt ttc cgc atg Tyr Leu y Phe Arg IVet 255 gtt ggt gcg gtg ttc atc Val Gy Ala Val Phe lie 270 275 cag gcg ggt gcg ttg agg Sn Ala G y Ala Leu Arg 290 ttc ttg agt ctg acg atg Phe Leu Ser Leu Thr IVet 305 aat ttg tgg gtc acg ttg Asn Leu Trp Val Thr Leu 320 ttc gca ctg cat tcc agt Phe Ala Leu His Ser Ser 335 aag gat cgc gcg gaa gcc Lys Asp Arg Ala G u Ala 350 355 gga tcc tcg gtg att ggt G y Ser Ser Val I e G y 370 Page 225 ccc Pr o ctt Leu cac Hi s act Thr gt a Val 180 at c SIe cag G n cat Hi s ggc Gy at t SIe 260 at g Met gag G u at c SIe gtt Val gct Al a 340 t cc Ser tgg Tr p 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 t ct gga tt c Ser a3 y Phe 375 t tg at t ct g Leu Il e Leu 390 agg ct t gcc Ar g Leu Al a tt t Ph e ct t Le u aac As n 410 cat Hi s 380 gga G31y aat As n seqI i st car r ect ed. t xt t tg ccg t gg t tg gcg t tc at t ggc t gg Leu Pro Trp Leu Ala Phe Ilie y Tr p 385 gt g ct g gcg at t t gt gt g acg ct g gca Val Leu Al a Ilie Cys Val Thr Leu Al a 400 405 t aat acgagt t tgt ccgt gt tt a 1267 1315 1359 <210O> 206 <211> 412 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 206 I'bt Ser G3 n Al a a3 y Hi s G3 u G3 y Phe Al a I'b t Leu Thr a3 n Al a Leu Thr a3 u Se r Al a Cys I Ie Val Pr o Val Leu Thr I Ie 1 00 Pr o Leu Val Pr o 115 a3 y Al a Leu Leu 130 a3 a 3 u I I e His 145 Al a a3 y As n Thr Leu Leu a3 u Val 1 80 Al a Al a Leu I I e 1 95 Ar g Lys Phe a31n 210 I'b t Al a Al a Hi s 225 Al a Phe Leu a3 y I I e Asp Ser li1e a3 u Ar g Al a Al a a3 y Leu Pr o Thr Leu Thr Val 70 Al a Ser Ilie Ser Leu Thr Asn Il e Thr Al a a3 y Thr 135 Pro Lys Asp 150 Val a31ya(1y 1 65 Thr Hi s Tr p Phe a3 y Val Pro Lys Asn 215 Tr p Ar g Asn 230 I'b t a3 y Thr 245 Lys Val a31u 10 a31y Th r Ar g Leu Al a Al a 40 I'b t Thr a3 u Ser Al a Thr Leu Ser a3 u 90 Leu Al a I I e 1 Al a Leu I I e 120 Pr o Al a Val I e a3 y Hi s Leu Thr a3 y 1 70 a3 n As n Al a 1 1I e I'b t Val 200 Il e Asn Leu Pr o Ar g Leu Phe Val Ser Al a As n Ph e a31u Thr Lys5 I Ie Le u Al a Al a 1 Ar g Le u ValI Ar g Al a 235 a31u Lys5 a31y a31y vb t a31y a31y Ar g 125 Thr ay le ay Le u 205 a31u Le u Hi s Al a Tyr Thr Al a a31y lie Le u Le u Tyr Al a 1 Se r Se r a31y Leu Tyr Asn Tyr Leu a31y 255 Page 226 Phe Val Al a Leu 305 Val Hi s Al a Val Al a 385 Cys Met SIle 275 Ar g Met Leu Ser Al a 355 Gy l Ie Thr Asp Tyr Lys Al a Al a 325 Ser Ser Val Tr p Al a 405 Phe Al a Gy 295 Met Phe Tr p Tyr Gy 375 I le Leu seql i st cor r ect ed. t xt Sy Leu Ser G u Val Leu 265 Sy Thr Trp Ser Ser Thr 280 285 Asn y Ser Thr Val lie 300 Ala Leu IVet Gy Ile Asn 315 Val Phe Thr Ala Ala Phe 330 li e G y Ile li e Al a Thr 345 Leu Phe Cys Tyr Tyr Val 360 365 Phe Ala Phe Thr His Leu 380 Leu Leu Leu Cys G y Val 395 Ala Arg Asn Ala Asn 410 Gy Al a Leu Leu Al a 335 Asp Ser Tr p Al a <210> 207 <211> 1215 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1192) <223> FRXA01873 <400> 207 ccgtcgttgc ccatggtcac agcctacatg cac acattttact aatatttgct gttggcgcat gat gat Asp gag Gu gcc Al a ccc Pr o acg Thr aag Lys gga Gy ctt Leu at g Met tcg Ser gag Gu cgc Ar g gct Al a gaa Gu acg Thr gca Al a aat Asn tt c Phe gag Gu act Thr 75 cac Hi s t ac Tyr aat Asn ttg Leu gga Gy gaa Gu aag Lys ggt Gy 45 gga Gy at g Met ggc Gy cgc Ar g ctt Leu at t SIle ttg Leu aaagtga atcaaaaaca gctatttcta gaactcc atg agc caa gca ata 115 IVet Ser Gn Ala lle 1 cac gaa ggc cac gaa ggc atc 163 His G u G y His G u G y lie 15 gct gtg ttt gcg atg ctg gcc 211 Ala Val Phe Ala IVet Leu Ala tat tgc acg cag gca ttg ctt 259 Tyr Cys Thr G n Ala Leu Leu acg ccc act gag tcc gcg ctg 307 Thr Pro Thr u Ser Ala Leu gcg ctg tgt att gtt ccg gcg 355 Ala Leu Cys Ile Val Pro Ala 80 Page 227 seql i st cor r ect ed. t xt tcg ata ctt tcg gag aaa ttt ggt cgc ggt Ser lie Leu Ser u Lys Phe Gy Arg y 95 cgg gtg Arg Val ctg aca att tca Leu Thr Ile Ser ct c Leu at t SIle ggc Gy aag Lys 150 ggc Gy cat Hi s ggc Gy aag Lys cgg Ar g 230 ggt Gy cag G n ct g Leu at c SIle tcg Ser 310 ct g Leu gga Sy ttg Leu gct Al a 120 cca Pr o at t SIle ct c Leu caa G n at c SIle 200 at c SIle cct Pr o ttt Phe ggg Gy ggg Gy 280 aat Asn gca Al a gt g Val at c SIle at c SIle at c l Ie gt g Val cat Hi s gga Gy 170 gca Al a gt g Val ct g Leu ttg Leu tcg Ser 250 agt Ser tgg Tr p t ca Ser at g Met acc Thr 330 at c SIle at c SIle ct g Leu gcg Al a gcg Al a 155 cgt Ar g ct g Leu gt g Val cgc Ar g gcg Al a 235 ct g Leu gaa G u agt Ser acg Thr ggg Gy 315 gcg Al a at c SIle gt g Val ct c Leu at g Met 140 at g Met at g Met ct g Leu ttg Leu cat Hi s 220 ttg Leu t ac Tyr gt g Val t cc Ser gtt Val 300 at t SIle gca Al a gca Al a gga Gy aga Ar g 125 acc Thr gga Gy at t SI e gga Gy ctt Leu 205 gag G u ctt Leu aac Asn ct g Leu acc Thr 285 at t SIle aat Asn ttt Phe acg Thr tta Leu 110 ggt Gy tgg Tr p at t SIle ccg Pr o agt Ser 190 ccc Pr o at t SIle ttt Phe t at Tyr gtt Val 270 cag G n ttc Phe aat Asn ttc Phe aag Lys 350 at t SIle ct c Leu ttg Leu t ac Tyr gcg Al a 175 tct Ser aag Lys tcg Ser ggt Gy ttg Leu 255 ggt Gy gcg Al a ttg Leu ttg Leu gca Al a 335 gat Asp ttg Leu caa G n tct Ser at c SIle 160 gga Gy at c SIle cag G n gcg Al a act Thr 240 ggt Sy gcg Al a ggt G y agt Ser tgg Tr p 320 ct g Leu cgc Ar g ccg Pr o ggt G y gag G u 145 gcg Al a ct a Leu gct Al a cgg Ar g at g Met 225 gcg Al a ttc Phe gt g Val gcg Al a ct g Leu 305 gt c Val cat Hi s ctt Leu gcg Al a 130 gaa G u gga G y ctt Leu gcg Al a aaa Lys 210 gct Al a ttt Phe cgc Ar g ttc Phe ttg Leu 290 acg Thr acg Thr t cc Ser 100 ccc Pr o ctt Leu cac Hi s act Thr gt a Val 180 at c l Ie cag G n cat Hi s ggc Sy at t SIle 260 at g Met gag G u at c SIle gtt Val gct Al a 340 aat Asn gct Al a ccc Pr o gt c Val 165 act Thr ttc Phe ccg Pr o tgg Tr p at g Met 245 gat Asp t at Tyr aag Lys gcg Al a gcc Al a 325 tcg Ser 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 gcg gaa gcc tcc agc Ala G u Ala Ser Ser 355 Page 228 seqi i st car r ect ed. t xt atg tat ttg ttc tgt gaa tac taggatcctc ggtgattggt tgg l'bt Tyr Leu Phe Cys G3 u Tyr 360 <210O> 208 <21 1> 364 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1215 <400> 208 l'bt Ser G3 n Ala I Ie Asp Ser Lys Val G31y Ph e Thr Thr Cys ValI Pr o G31y (31u 145 A a Le u A a Ar g vb t 225 A a Ph e ValI A a (31u vb t A a Ser ValI Thr ValI 115 Le u I Ie As n (31u Le u 1 95 Ph e A a Le u vb t I Ie 275 Ar g le A a Le u Le u A a Ser As n A a Pr o ValI 1 65 Thr Ph e Pr o Tr p vb t 245 As p Tyr Ly s Ar g G31y Thr ValI I Ie Thr Thr Thr 135 As p G31y Tr p ValI As n 215 As n Thr Ph e A a G31y 295 Thr A a Thr A a Ser A a 1 Le u A a G31y Thr As n 1 vb t As n Ar g ValI Le u 265 Thr G31y G31u 10 Ar g A a G31u Thr G31u 90 lie lIe ValI Hi s G31y 1 70 A a ValI Le u Le u Ser 250 Ser Tr p A a As n Ph e G31u Thr Ly s I Ie Le u A a A a 1 Ar g Le u ValI Ar g A a 235 Le u G31u Ser G31u Ly s G31y G31y vb t G31y G31y Ar g 125 Thr Gy le Gy Le u 205 G31u Le u As n Le u Thr 285 Hi s A a Tyr Thr A a G31y lie Le u Le u Tyr A a 1 Ser Ly s Ser G31y Le u 255 G31y A a Ser Thr Val I I e Phe Leu Ser 300 Page 229 seqI i st car r ect ed. t xt Leu Thr [Vbt I Ie Al a Ser [Vbt Al a Leu [Vbt G31y I Ie As n As n Le u Tr p 305 310 315 320 Val Thr Leu Val Al a Leu Phe Val Phe Thr Al a Al a Phe Phe Al a Leu 325 330 335 Hi s Ser Ser Al a Ser G31y Tr p I Ie 31y Ilie Ilie Ala Thr Lys Asp Ar g 340 345 350 Al a (3 u Al a Ser Ser I'b t Tyr Leu Phe Cys G3 u Tyr 355 360 <210O> 209 <21 1> 1572 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (1549) <223> RXN00034 <400> 209 t aaatt t tgt ggcact cccc acat t tct at caat ctat ag tt t tgcaagt t tct at agat t gat agaaaa gggagt t tag aaagt at gac t taaagt cga at g t ct t ac aca t ct 11! I'bt Ser Tyr Thr Ser tt t Ph e ac a Thr gca Al a gcc Al a gca Al a aac As n ac c Thr gcc Al a act Thr ggc (31y ggc G31y t gg Tr p g at As p gcg Al a gt c ValI ct g Le u at g vb t at g vb t 120 cgc Ar g gga (31y g at As p tt t Ph e ggg G31y tt c Ph e ggc G31y t ct Ser at c I Ie gca Al a cga Ar g t gc Cys aaa Lys5 gct Al a at g vb t t gt Cy s cga Ar g 75 ct c Le u acc Thr t cc Ser gca Al a gcg Al a gcc Al a c ag O31n ag c Ser gga G31y gt a ValI ct c Le u ggc G31y ct a Le u gt c ValI 140 ct t Le u ct c Le u t ca Ser g at As p 45 ac a Thr cga Ar g at c I Ie cga Ar g gca Al a 125 t cc Ser tt c Ph e at c ggc le G31y 15 acc ct a Thr Leu ggc acc 03 y Thr t tt at c Phe I I e at c at g I e I'b t gcc acg Al a Thr 95 gt t ct c Val Leu 110 t t a gt t Leu Val at t t gg I Ie Tr p gcg ggt Al a 03 y at a I Ie aat As n at g vb t gt c ValI at g vb t gca Al a c aa O31n aag Lys5 t cc Ser 1 gt t ValI at c I Ie aac As n gca Al a at t I Ie ac g Thr gga G31y ac a Thr at t I Ie 145 tt a Le u ggc G31y at a lIe gcc Al a ggc G31y act Thr ct g Le u t at Tyr 130 ggt (31y t ca Ser cca Pr a gct Al a ggc G31y aac As n t ct Ser gca Al a 115 t gg Tr p t ca Ser gt t ValI g at As p gt c ValI ggc G31y at c I Ie tt a Le u 1 00 gca Al a tt a Le u t gg Tr p ctt gtt gta gca agc ccc Leu Val Val Al a Ser Pr o Page 230 NO seql i st cor r ect ed. t xt 150 155 160 165 ttt ggt tgg aga gga atc ttc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe G y Trp Arg G y Ile Phe Ala Leu Cys Ala I le Val Ser Ile Val ,C 170 175 180 4 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 Ala I I e Aa Leu Thr Arg His I I e Pro G uSer Arg Pro Ala G n Ser 185 190 195 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 Il1e G y /b t His Leu Asp Trp Ser G y I I e I I e Val Leu Ala Leu Ser 200 205 210 00 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tca ctt ggc tgg 787 S Val Leu Ser Leu u Leu Phe Ile Thr n 3y 3 uSer Leu y Trp 215 220 225 .C acg cac tgg atg acc tgg act ctc ctt gcc gtt tct ttg aca ttt ctt 835 SThr His Trp 'b t Thr Trp Thr Leu Leu Ala Val Ser Leu Thr Phe Leu S230 235 240 245 gca gtt ttc gtc ttc att gaa cgc atc gcc agc tgg cca gtt ctc gac 883 Ala Val Phe Val Phe Ile u Arg Ile Ala Ser Trp Pro Val Leu Asp 250 255 260 ttc aac ctt ttc aaa gac cac gcc ttc agc ggt gcg acc atc acc aac 931 Phe Asn Leu Phe Lys Asp His Ala Phe Ser G y Ala Thr Ile Thr Asn 265 270 275 ttc att atg agc gct act ggc gga gta gtt g gt gc gtc atg t gtatggggtt 979 Phe Ile 'b t Ser Ala Thr G y G y Val Val Ala Val Val 'b t Trp Val 280 285 290 cag caa atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc agc 1027 n O n 'b t y Trp y Val Ser Pro Thr Ile Ser y Leu Thr Ser 295 300 305 atc ggc ttc gca gcc ttt gtc atc ctt ttc att cga gtt gga gaa aag 1075 Ile y Phe Ala Ala Phe Val Ile Leu Phe Ile Arg Val y u Lys 310 315 320 325 gcc atg cag aaa gtt ggc gcc cga gca gtg atc atc acc gct ggc atc 1123 Ala Ibt n Lys Val y Ala Arg Ala Val Ile Ile Thr Ala G y Ile 330 335 340 ttg gta gcg acc gcg acc gcc ctc cta atg atc acc gcg gtc agc gag 1171 Leu Val Ala Thr Ala Thr Ala Leu Leu 'b t Ile Thr Ala Val Ser u 345 350 355 tca acg tac atc gtc atc tcc ctc gcc ggc ttc tcc ctt tat ggc ctt 1219 Ser Thr Tyr Ile Val Ile Ser Leu Ala G y Phe Ser Leu Tyr G y Leu 360 365 370 ggc ctc gga ctc ttc gcc acc cca gtc acc gat act gcg ctt gga aca 1267 G y Leu G y Leu Phe Ala Thr Pro Val Thr Asp Thr Ala Leu G y Thr 375 380 385 ctt ccc aaa gac cgt acc ggc gct ggt gca ggt gta ttc aag atg tcc 1315 Leu Pro Lys Asp Arg Thr G y Ala G y Ala G y Val Phe Lys 'b t Ser 390 395 400 405 tct tcc ctc ggc gca gca ctc ggc atc gca atc tcc act tca gtg ttc 1363 Ser Ser Leu G y Ala Ala Leu G y Ile Ala le Ser Thr Ser Val Phe 410 415 420 ctc gca ctt cgc gac ggc acc tcc atc aac tcc gac gtc gca ctc gcc 1411 Leu Aa Leu Arg Asp y Thr Ser Ile Asn Ser Asp Val Aa Leu Al a Page 231 seql i st cor r ect ed. t xt 425 430 435 gga aca gtt tca ctt ggc atc aac gtt gta ttc gca gca aca gcc acc Sy Thr Val Ser Leu G y lie Asn Val Val Phe Ala Ala Thr Ala Thr 440 445 450 atc acc gca gca gtc ctt att cca aaa gcc gct ggc aaa gtc tca caa Ile Thr Ala Ala Val Leu lie Pro Lys Ala Ala y Lys Val Ser G n 455 460 465 acc agc atc acc ctt cct gag cca gct atc gct gta aaa atc Thr Ser lie Thr Leu Pro u Pro Ala lle Ala Val Lys Ile 470 475 480 t aaaact t ca ccaggacaga t aa <210> 210 <211> 483 <212> PRT <213> Corynebacter i um gl ut ai cum 1459 1507 1549 1572 <400> 210 Met Ser Tyr Thr Ser Phe Lys 1 5 Val Leu Ser Val Leu Thr Phe lie y Pro Asp IVet Ala Thr Asn lie Ala Val Val Ala Ala Al a Al a G y G y I e Al a Asp 70 I le G y Asn I e Leu Asn Il e Thr Thr Ser Leu Ala Thr G n 100 G y Leu Al a Al a Al a Al a lie 115 Thr Tyr Trp Leu y Thr Asp 130 135 I e y Ser Trp Gy Gy Thr 145 150 Val Val Ala Ser Pro Phe y 165 Ile Val Ser Ile Val Ala Ile 180 Arg Pro Ala n Ser Ile y 195 Val Leu Ala Leu Ser Val Leu 210 215 G u Ser Leu G y Trp Thr Hi s 225 230 Asp Asp 10 Leu Phe Leu G y Leu Phe Phe G y G y Ser 90 Val lile 105 Ser Al a G n Arg Phe Cys Arg G y 170 Leu Thr 185 Hi s Leu Leu G u Met Thr Lys Ala Leu Ile G y Ie Al a et Cys Ar g Leu Thr Ser Al a Al a 155 I le Ar g Asp Leu Tr p 235 Ser Asp Thr Ar g SIle Ar g Al a 125 Ser Phe Al a SIle Ser 205 I le Leu Thr SIe Met Thr Leu Val Tr p Gy Cys 175 Su lie G n Thr Leu Leu Ala Val Page 232 Ser Leu Thr Phe Leu Al a Val Ph 245 seql i st cor r ect ed. t xt e Val Phe lie Ou Arg lle Al a Ser 255 Tr p Al a Val Ser 305 Ar g SIle Thr Ser Thr 385 Val Ser Asp Al a Gy 465 Val Val l Ie 275 Met Leu Gy Al a Val 355 Tyr Leu Lys Ser Al a 435 Thr Val SIe Asp Asn Val Ser Lys 325 l le G u Leu Thr Ser 405 Phe Al a Thr G n Asn SIle G n 295 Gy et Val Thr Leu 375 Pr o Ser Al a Thr Thr 455 Ser Leu bet 280 Met Phe G n Al a Tyr 360 Gy Lys Leu Leu Val 440 Al a I le Phe 265 Ser Gy Al a Lys Thr 345 I le Leu Asp Gy Ar g 425 Ser Al a Thr 250 Lys Al a Tr p Al a Val 330 Al a Val Phe Ar g Al a 410 Asp Leu Val Leu Asp Thr Gy Phe 315 Gy Thr SIle Al a Thr 395 Al a Gy Gy Leu Pr o 475 Hi s Gy Val 300 Val Al a Al a Ser Thr 380 Gy Leu Thr SIle SIle 460 G u Al a Gy 285 Ser SIle Ar g Leu Leu 365 Pr o Al a Gy Ser Asn 445 Pr o Pr o Phe 270 Val Pr o Leu Al a Leu 350 Al a Val Gy SIle SIle 430 Val Lys Al a Ser Val Thr Phe Val 335 bet Gy Thr Al a Al a 415 Asn Val Al a SIe <210> 211 <211> 1045 <212> DNA <213> Corynebacter i um gl utami cum <220> <221> CDS <222> (101)..(1045) <223> FRXA02273 <400> 211 taaattttgt ggcactcccc acatttctat caatctatag aaagtatgac ttaaagtcga ttttgcaagt ttctatagat tgatagaaaa gggagtttag atg tct tac aca tct 115 Met Ser Tyr Thr Ser 1 ttt aaa ggc gat gat aaa gcc ctc atc ggc ata gtt tta tca gtt ctc 163 Phe Lys Gy Asp Asp Lys Ala Leu lie Gy lie Val Leu Ser Val Leu Page 233 IO seql i st cor r ect ed. t xt 15 aca ttt tgg ctt ttt gct cag tca acc cta aat atc ggc cca gat atg 211 Thr Phe Trp Leu Phe Al a G n Ser Thr Leu Asn Ile G y Pro Asp IVet 30 d) 4 gca act gat tta ggg atg agc gat ggc acc atg aac ata gct gtc gtg 259 SAla Thr Asp Leu G y It Ser Asp G y Thr Iet Asn lle Ala Val Val 40 45 gcc gcc gcg tta ttc tgt gga aca ttt atc gtc gca gcc ggc ggc atc 307 Ala Ala Ala Leu Phe Cys y Thr Phe I e Val Ala Ala y y y lle 60 00 gca gat gtc ttt ggc cga gta cga atc atg atg att ggc aac atc ctt 355 SAla Asp Val Phe y Arg Val Arg Il e Iet Iet I Ie y Asn Ile Leu S 70 75 80 (N aac atc ctg gga tct ctc ctc atc gcc acg gca acg act tct tta gcc 403 SAsn Ile Leu y Ser Leu Leu lie Ala Thr Ala Thr Thr Ser Leu Ala 95 100 acc caa atg gtg atc acc ggc cga gtt ctc caa gga ctg gca gca gcg 451 Thr n Met Val Ile Thr y Arg Val Leu n y Leu Ala Ala Ala 105 110 115 gcc atc atg tct gca tcc cta gca tta gtt aag aca tat tgg tta ggt 499 Ala lle IVet Ser Ala Ser Leu Ala Leu Val Lys Thr Tyr Trp Leu G y 120 125 130 act gac cgc caa cga gca gtc tcc att tgg tcc att ggt tca tgg ggt 547 Thr Asp Arg G n Arg Al a Val Ser Ile Trp Ser Ile y Ser Trp y 135 140 145 ggc acc gga ttc tgc gcg ctt ttc gcg ggt ctt gtt gta gca agc ccc 595 Sy Thr y Phe Cys Al a Leu Phe Ala y Leu Val Val Ala Ser Pro 150 155 160 165 ttt ggt tgg aga gga atc ttc gcc ctc tgc gcg atc gtc tcc atc gtt 643 Phe y Trp Arg y lle Phe Ala Leu Cys Ala Ile Val Ser lie Val 170 175 180 gct att gcc ctt acc cgc cac atc ccg gaa tcc cgt ccg gct caa tcc 691 Ala lie Ala Leu Thr Arg His lie Pro G u Ser Arg Pro Ala G n Ser 185 190 195 att ggc atg cat ttg gat tgg agt ggc atc atc gtt ctt gcc ctc agt 739 IIe G y Iet His Leu Asp Trp Ser G y lie lie Val Leu Ala Leu Ser 200 205 210 gtt cta tct ctt gaa ttg ttt att acc caa ggt gaa tca ctt ggc tgg 787 Val Leu Ser Leu G u Leu Phe Ile Thr G n y u Ser Leu y Trp 215 220 225 acg cac tgg atg acc tgg act ctc ctt gcc gtt tct ttg aca ttt ctt 835 Thr His Trp IVet Thr Trp Thr Leu Leu Ala Val Ser Leu Thr Phe Leu 230 235 240 245 gca gtt ttc gtc ttc att gaa cgc atc gcc agc tgg cca gtt ctc gac 883 Ala Val Phe Val Phe Ile G u Arg Ile Ala Ser Trp Pro Val Leu Asp 250 255 260 ttc aac ctt ttc aaa gac cac gcc ttc agc ggt gcg acc atc acc aac 931 Phe Asn Leu Phe Lys Asp His Ala Phe Ser G y Ala Thr lie Thr Asn 265 270 275 ttc att atg agc gct act ggc gga gta gtt gcc gtt gtc atg tgg gtt 979 Phe Ile Met Ser Al a Thr G y G y Val Val Ala Val Val Met Trp Val Page 234 seqI i st car r ect ed. t xt 280 285 290 cag caa atg gga tgg ggt gtc tcc cca aca atc tcg gga ctc acc agc O31n O31n 'b t G31y Tr p G31y Val Ser Pro Thr Ilie Ser G31y Leu Thr Ser 295 300 305 at c ggc t tc gca gcc ttt Il e 03 y Phe Al a Al a Ph e 310 315 <210> 212 <211> 315 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1 027 1 045 <400> 212 I'bt Ser T~ Val Leu SE I e 03 y Pr As n II e Al Al a Al a 03 li1e G31y A,, Thr Thr SE O3 y Leu Al 11 Thr Ty r Tr 130 1 e 03 y SE 145 Val Val Al I Ie Val SE Ar g Pr o Al Val Leu Al 210 O3 u Ser LE 225 Ser Leu T~ Tr p Pr o V, Thr Ser Phe a y a '5 a ar a Le u vb t ValI I Ie Le u Al a Al a G31y G31y Pr o 1 65 ValI Ser Ser Tr p Le u 245 As p Thr Al a Al a Al a 70 As n Thr Al a Thr G31y 150 Ph e Al a I Ie ValI Thr 230 Al a Ph e Ly s Ph e Thr Al a As p I Ie O31n I Ie As p 135 Thr G31y I Ie G31y Le u 215 Hi s ValI As n Asp Asp 10 Leu Phe Leu 03 y Leu Phe Phe 03 y O3 y Ser 90 Val I Ie 1 Ser Al a O31n Ar g Phe Cys Ar g O31y 1 70 Leu Thr 1 Hi s Leu Leu 03 u I'b t Thr Val Phe 250 Phe Lys 265 Lys Al a Leu Il e O31y le Al a vb t Cys Ar g 75 Le u Thr Ser Al a Al a 1 55 li1e Ar g As p Le u Tr p 235 li1e Ser As p Thr Ar g I Ie Ar g Al a 125 Ser Ph e Al a I Ie Ser 205 li1e Le u Ar g Le u Thr I Ie vb t Thr Le u ValI Tr p O31y Cys 1 Ou le O31n Al a Al a 255 As n vb t ValI vb t Al a O31n Ly s Ser Le u 1 Al a Ser I Ie O31y ValI 240 Se r Asp Hi s Al a Phe Ser 0 y 270 Page 235 seql i st cor r ect ed. t xt Ala Thr lie Thr Asn Phe lie bet Ser Ala Thr Oy Oy Val Val Ala 275 280 285 Val Val Met Trp Val n G n Met G y Trp G y Val Ser Pro Thr Ile 290 295 300 Ser y Leu Thr Ser I le y Phe Ala Ala Phe 305 310 315 <210> 213 <211> 826 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(826) <223> RXN03075 <400> 213 tgtgcaaaat tgcattcagg ctgaaaaatt cctaaaggga ctccgtccga ataattggaa agcccagaag aacagtcaac tcctagatta aaggataatc gtg gcg Val Ala t at Tyr gt c Val gcc Al a acg Thr aca Thr acc Thr gag G u cct Pr o gag G u at t SIle 150 tta Leu ct c Leu cca Pr o at g Met gct Al a act Thr cgg Ar g ttg Leu 120 cag G n cca Pr o t cc Ser at t SIle tcg Ser aag Lys act Thr cct Pr o act Thr gcg Al a gt a Val agt Ser acg Thr ct c Leu agt Ser at g Met ggt G y 75 gag G u ggt Gy cag G n cct Pr o gca Al a 155 gcc Al a at a SIle agc Ser cag G n tct Ser gtt Val gt g Val ggt G y gcg Al a 140 gat Asp caa G n at c I e tct Ser cgt Ar g gt c Val gct Al a aag Lys 110 gct Al a aag Lys act Thr aag Lys 15 acg Thr at c SIle ttc Phe at t SIle gaa G u 95 gat Asp gct Al a at c SIle acc Thr aaa Lys acg Thr cct Pr o cct Pr o cag G n 80 gt a Val gct Al a cag G n gcc Al a ggc 10y 160 1 tgg Tr p ct g Leu ggt G y ggt G y gca Al a aac Asn gat Asp at g Met gca Al a 145 at c SIle ccg Pr o gcg Al a ctt Leu tcg Ser ccg Pr o cag G n t cc Ser acc Thr 130 gat Asp at c SIle aaa ttc ctg Lys Phe Leu ttt ctt gcg Phe Leu Al a ggg ctg t at G y Leu Tyr gat tct gtc Asp Ser Val gat gat gca Asp Asp Al a gaa ggc aag G u G y Lys atg ctt gat Iet Leu Asp 100 gtt gtg gat Val Val Asp 115 cca gcc ct g Pro Al a Leu att gag tcg Ile u Ser t cg at g act Ser IVet Thr 165 ttt gat gca gat tct gcc atg gat ata tcc gca gag gat cgt gag aag Page 236 Phe Asp A a Asp Ser Al a lbt Asp 1 1 70 gt c acc aat at t ct t gat gaa t ac g Val Thr As n I Ie Leu As p (31u Ty rA 185 1 t ac aac ggc aac gt g t tt ggc gca g Ty r As n G31y As n Val Phe (31y Ala P 200 205 t ct gag ct c at c ggc ct g ct g gt g g Ser G31u Leu I Ie G31y Leu Leu Val P 215 220 t tc ggt t cg t tc at c gct gcc ggt a Phe G31y Ser Phe I Ie Ala Ala Gy1 y 230 235 <210O> 214 <21 1> 242 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn lIi st car r ect ed. t xt Ie Ser Ala G31u Asp Ar g G31u Lys 175 180 iat gac ggc gat ct g act gt t gt c osp As p G31y As p Leu Thr Val Val 90 195 c t gca acc agc t tg gac at g acc Il a A a Thr Ser Leu As p l'b t Thr 210 c t gcg gt c gt t ct t at c gt g acc I a Ala Val Val Leu I Ie Val Thr 225 .t g ccg ct g at c t ct /bt Pr o Leu I Ie Ser 240 <400> 214 Val A a Lys Phe Tr p Pr o Phe Leu Leu A a G3 y Leu G3 y Leu Asp Ser G3 y Se r Asp Asp Ala Pr o G31u G31y As n G3 n l'b t Leu 1 00 As p Ser Val Val 115 l'b t Thr Pr o Ala 130 A a As p I I e a3 u 145 1 1 e I I e Ser l'b t a31u As p Ar g a31u 1 80 As p Leu Thr Val 1 95 Ser Leu As p l'b t 210 Le u A a Tyr ValI A a Ly s As p As p Le u Ser Thr 1 65 Ly s ValI Thr Tyr Lys Leu a3 y Tr p Ly s Thr Ser Le u ValI ValI A a 135 Ser As p Thr As n a31u 215 ValI 25 Thr a31u Pr o As p A a 1 05 A a a31y Le u As p I Ie 1 85 As n I Ie Ser 10 I Ie Ser Ly s Thr Pr o 90 Thr A a ValI Ser Ser 1 70 Le u ValI Th r A a Ty r a3 n Lys Lys Le u Ser vb t a31y a31u a31y a31n Pr o A a 1 A a As p Ph e a31y Ph e a31u ValI a31y Le u ValI 125 a31u a31u As p Tyr A a 205 lIe Ser Ar g ValI A a Ly s 110 A a Ly s Thr I Ie As p 1 A a Thr I Ie Ph e I Ie a31u As p A a I Ie Thr Se r 1 As p A a a31y Leu Leu Val Ala Ala Val 220 Page 237 seqi i st car r ect ed. t xt Val Leu lieVal Thr Phe Gy Ser Phe IlieAla Ala Gy l'bt Pro Leu 225 230 235 240 1 1 e Ser <210O> 215 <21 1> 826 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (826) <223> FRXA02907 <400> 215 t gt gcaaaat t gcat tcagg ct gaaaaat t cct aaaggga agcccagaag aacagtcaac tcctagatta aaggataatc ct ccgt ccga at aat tggaa gt g gcg aaa t tc ct g 11! Val A a Lys Phe Leu t at Tyr gt c ValI gcc A a ac g Thr ac a Thr ac c Thr gag O31u cct Pr a gag O31u at t I Ie 1 50 tt t Ph e tt a Le u ct c Le u cca Pr a at g vb t gct A a act Thr cgg Ar g tt g Le u 120 c ag O31n cca Pr a gca A a t cc Ser at t I Ie t cg Ser aag Ly s act Thr cct Pr a act Thr gcg A a gt a ValI agt Ser t ct Ser 1 70 ac g Thr ct c Le u ag t Ser at g vb t ggt G31y 75 gag O31u ggt O31y cag O31n cct Pr a gca A a 155 gcc A a gcc A a at a I Ie ag c Ser c ag O31n t ct Ser gt t ValI gt g ValI ggt G31y gcg A a 140 g at As p at g vb t c aa G n at c lIe t ct Ser cgt Ar g gt c ValI gct A a aag Ly s 110 gct A a aag Ly s act Thr at a I Ie aaa Ly s ac g Thr cct Pr a cct Pr a c ag O31n 80 gt a ValI gct A a c ag O31n gcc A a ggc O31y 1 60 gca A a 1 t gg Tr p ct g Le u ggt O31y ggt O31y 65 gca A a aac As n g at As p at g vb t gca A a 145 at c I Ie gag O31u ccg Pr a gcg A a ct t Le u 50 t cg Ser ccg Pr a c ag O31n t cc Ser ac c Thr 130 g at As p at c I Ie g at As p tt t Ph e ggg O31y 35 g at As p g at As p g aa O31u at g Nibt gt t ValI 115 cca Pr a at t I Ie t cg Ser cgt Ar g ct t Le u 20 ct g Le u t ct Ser g at As p ggc O31y ct t Le u 1 00 gt g ValI gcc A a gag O31u at g vb t gag O31u 1 gcg A a t at Tyr gt c ValI gca A a aag Ly s g at As p g at As p ct g Le u t cg Se r act Th r 1 aag Ly s Page 238 gt c acc aat at t ct t gat gaa t ac g Val Thr As n I Ie Leu As p O31u Ty rA 185 1 t ac aac ggc aac gt g t tt ggc gca g Ty r As n G31y As n Val Phe G31y Ala A 200 205 t ct gag ct c at c ggc ct g ct g gt g g Ser O31u Leu I Ie G31y Leu Leu Val P 215 220 t tc ggt t cg t tc at c gct gcc ggt a Phe G31y Ser Phe I Ie Ala Ala Gy1 y 230 235 <210O> 216 <21 1> 242 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn lIi st car r ect ed. t xt iat gac ggc gat ct g act gt t gt c osp Asp G31y Asp Leu Thr Val Val 90 195 ict gca acc agc t tg gac at g acc Il a A a Thr Ser Leu As p N/t Thr 210 ict gcg gt c gt t ct t at c gt g acc I a Ala Val Val Leu I Ie Val Thr 225 .t g ccg ct g at c t ct /bt Pr o Leu I Ie Ser 240 <400> 216 Val A a Lys Phe Leu Tyr Lys Leu G3 y Ser Thr A a Tyr a3 n Lys Lys Tr p Le u G31y G31y A a As n As p vb t A a 145 li1e O31u As p Ser ValI 225 Ph e G31y As p As p O31u vb t ValI 115 Pr a I Ie Ser Ar g Thr 1 95 As p I Ie A a Tyr ValI A a Ly s As p As p Le u Ser Thr 1 65 Ly s ValI Thr Thr Tr p Ly s Thr Ser Le u ValI ValI A a 135 Ser As p Thr As n O31u 215 G31y Le u Pr a 40 vb t A a Thr Ar g Le u 120 O31n Pr a A a As n G31y 200 Le u Ser 10 Val I Ie Thr Ser 03 u Lys Pr o Thr As p Pr o 90 A a Thr 1 05 A a A a 03 y Val Leu Ser Asp Ser 1 70 1 1 e Leu 1 85 As n Val I Ie G31y Phe I I e Le u Ser vb t G31y 75 O31u G31y O31n Pr a A a 1 55 A a As p Ph e Le u I Ie Ser O31n Ser ValI ValI G31y A a 140 As p vb t (31u G31y Le u 220 G31y Ph e (31u ValI G31y Le u ValI 125 (31u (31u As p Tyr A a 205 ValI lIe Ser Ar g ValI A a Ly s 110 A a Ly s Thr I Ie As p 1 A a A a Thr I Ie Ph e I Ie (31u As p A a I Ie Thr Ser 1 As p A a A a Thr Pr a Pr a O31n ValI A a O31n A a O31y 1 A a O31y Th r ValI Ala Ala Oy l'bt Pro Leu 235 240 Page 239 seql i st car r ect ed. t xt I I e Ser <210O> 217 <21 1> 2313 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2290) <223> RXA00479 <400> 217 t agat cccaa ggct caaaat t tat tact ta aacaagt tga ct tagaact a acct t tacgc ct t taacgga agt gaat t tg gcaact agcc agccgcaaat ac a Thr tt t Ph e ggc G31y ac c Thr tt g Le u gt c ValI gt t ValI cct Pr o ct c Le u ac c Thr 1 50 ccg Pr o ggg (31y gag O31u ccc Pr o cct Pr o t at Tyr g at As p g at As p gct A a at t I Ie 120 cca Pr a ac c Thr ggg G31y ct c Le u aag Lys5 tt g Le u tt t Ph e cca Pr a act Thr ccc Pr a ct t Le u t cc Ser gcg A a c aa O31n ac c Thr 1 70 gca A a aag Lys5 ct a Le u ggc G31y gaa O31u g at As p 75 t t a Le u t ca Ser gag O31u gag O31u ac g Thr 155 gct A a gt c ValI aaa Lys5 at t I Ie aag Lys5 t ct Ser t ct Ser ac a Thr g aa O31u g ac As p ct g Le u 140 ccg Pr a g at As p gcc A a t ct Ser tt a Le u gt t ValI 45 gcc A a g aa O31u c ag O31n tt a Le u 99c O31y 125 ac g Thr g ac As p ct c Le u tt g Le u ggt O31y gt t ValI agt Ser g at As p t cc Ser c aa O31n g ac As p 110 ag a Ar g g aa (31u t at Tyr ag c Ser gct A a 1 cct Pr a 15 t gg Tr p gag (31u gcc A a at c I Ie g ac As p 95 at a I Ie gct A a ag c Ser gt g ValI gca A a 1 75 gcc A a cct Pr a ct t Le u gt c ValI act Thr cca Pr a 80 at c I Ie gt t ValI gt c ValI gt c ValI ag c Ser 1 60 gct A a gt c ValI at g vb t 1 cgc Ar g gt a ValI t cc Ser c aa O31n 65 gcc A a ac a Thr t cc Ser c aa O31n gag O31u 145 ac c Thr tt c Ph e ct t Le u tt g Le u gga O31y t cc Ser 50 gt a ValI at t I Ie c aa O31n g at As p gt g ValI 130 aag Lys5 t at Tyr gcg A a gt c ValI ag a Ar g 20 gga O31y ag c Ser g aa O31u gt a ValI aat As n 1 00 gt c ValI gt c ValI t ct Ser ac c Thr at t I Ie 1 ct t Le u at c I Ie gt c ValI c ag O31n c ag O31n at g vb t g aa O31u t cc Ser ccc Pr a gag O31u gga O31y 1 g at As p gt c ValI t ct act agc at c Ser Thr Ser I Ie Page 240 seql i st cor r ect ed. t xt at c SIle ttg Leu tgg Tr p 230 ct g Leu ttc Phe aaa Lys gtt Val t cc Ser 310 tct Ser gcc Al a aaa Lys gt g Val ct c Leu 390 caa G n gct Al a at c SIle aac Asn t at Tyr 200 gcg Al a at c SIle at t SIle gaa G u at c SIle 280 gcg Al a ct a Leu ctt Leu tgg Tr p gac Asp 360 cag G n ggt Sy gac Asp ggc G y gat Asp 440 aat Asn tcc ttc att Ser Phe II e act Thr ct g Leu gcc Al a 250 tta Leu gca Al a ct c Leu cca Pr o ct g Leu 330 aag Lys ccc Pr o cct Pr o gct Al a gtt Val 410 cac Hi s aca Thr gag G u gt a Val ctt Leu 235 gcc Al a cgc Ar g tcg Ser ctt Leu gt a Val 315 ct a Leu cga Ar g gcc Al a cgt Ar g tt c Phe 395 ct g Leu tt c Phe cag G n act Thr gct Al a 220 tcg Ser acc Thr gtt Val gt g Val tgt Cys 300 gct Al a cca Pr o cca Pr o agc Ser gca Al a 380 gtt Val ggt G y ccc Pr o gca Al a gt a Val 460 ct g Leu 205 ct a Leu ggt G y gac Asp caa G n gaa G u 285 ttg Leu tcg Ser gcc Al a aaa Lys ggg Gy 365 at c l Ie ccc Pr o t cc Ser ggt G y gca Al a 445 act Thr ccc atc gcc gtg ctt gcc Pro lie Ala Val Leu Ala 210 ttg gtg gtg tgg tgg cta Leu Val Val Trp Trp Leu 225 cag act caa ggc atc ctc G n Thr n G y Ile Leu 240 tac tca ttg cta tac gtt Tyr Ser Leu Leu Tyr Val 255 caa gat aaa ggg ata gcc G n Asp Lys G y I e Al a 270 275 ccc att ctt gcc tcg ggc Pro lie Leu Ala Ser y 290 cta ttt agt gat ttg aaa Leu Phe Ser Asp Leu Lys 305 gtg ggc att att ttt gca Val y Ile Ile Phe Ala 320 ctg ctg ttt gta ttc ggt Leu Leu Phe Val Phe G y 335 tac gaa cct gaa aaa gcc Tyr G u Pro G u Lys Ala 350 355 atc tgg tca aaa gtg gct lie Trp Ser Lys Val Ala 370 tgg gta tct aca ctt att Trp Val Ser Thr Leu Ile 385 aca cta aaa gcg gac ggt Thr Leu Lys Ala Asp y 400 t ct gaa gca cgt gat ggc Ser G u Ala Arg Asp y 415 gga t cc ggc agt cct gct G y Ser G y Ser Pro Ala 430 435 cag gct gct gac gta gtc G n Ala Ala Asp Val Val 450 gta act agt gct gac t cc Val Thr Ser Ala Asp Ser acc Thr gct Al a ttc Phe gct Al a 260 aca Thr agc Ser tct Ser at g Met cgg Ar g 340 cgt Ar g gat Asp gt g Val gt g Val cag G n 420 t at Tyr ctt Leu agt Ser aag Lys at t SIe 245 cgt Ar g ggg G y act Thr aac Asn ctt Leu 325 gt g Val gcg Al a tta Leu ctt Leu t cc Ser 405 cag G n at t SIe aac Asn 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 ccc tct Pro Ser 465 Page 241 seql i st cor r ect ed. t xt ggc Gy 470 aca Thr aca Thr agt Ser ggt Sy cgc Ar g 550 at g Met acc Thr aat Asn gga Gy gt c Val 630 at t SIle gt a Val ttc Phe acc Thr gga G y 710 gcc Al a cca Pr o gt c Val cgc Ar g 520 act Thr ct g Leu ttg Leu gt g Val gtt Val 600 gt a Val cga Ar g cga Ar g ct c Leu gca Al a 680 ct c Leu aaa Lys at c l Ie ccg Pr o 490 gca Al a act Thr act Thr at c SIle cgg Ar g 570 ttt Phe agt Ser tta Leu cgt Ar g ct g Leu 650 gca Al a at t l Ie cgc Ar g tgg Tr p ct a Leu 730 acc Thr 475 gt a Val cca Pr o ttt Phe t cc Ser cca Pr o 555 tct Ser gct Al a tt c Phe gt a Val gaa Gu 635 aca Thr acg Thr gcc Al a gcc Al a tgg Tr p 715 gct gac ggt Al a Asp G y gtt gta Val Val gat t cc Asp Ser gca gat Al a Asp 525 gta gac Val Asp 540 att gta Ile Val att gtc Ile Val act gct Thr Al a cca gga Pro G y 605 gcc ttg Al a Leu 620 gaa acc G u Thr gt a acc Val Thr ttc gca Phe Al a ttc att Phe Il e 685 ttc ttg Phe Leu 700 ccg t ca Pro Ser gaa Gu gaa Gu 510 gaa Gu act Thr ttg Leu gca Al a tta Leu 590 gca Al a ggc Gy aaa Lys ggc G y gca Al a 670 gt c Val gt g Val aaa att gtg Ile Val 480 ggg caa Gy n 495 gaa gct u Al a aat at a Asn II e aac gat Asn Asp ctg gt c Leu Val 560 cca ct c Pro Leu 575 ggc gtg G y Val gac ccc Asp Pro atc gac I e Asp acc cac Thr His 640 gga gt a G y Val 655 ct c t at Leu Tyr gct ttt Al a Phe cct gct Pro Al a ttg tcc ccg tta ggt Pro Leu G y gtc ctt tta Val Leu Leu caa aaa gct G n Lys Al a 515 t ca gcg gt a Ser Al a Val 530 gcc t cc at c Ala Ser Ile 545 att ttg gtt I e Leu Val ct g ct a gt a Leu Leu Val gct gct tta Al a Al a Leu 595 gca gt a cct Al a Val Pr o 610 tac aac at t Tyr Asn Ile 625 ggc aca aga G y Thr Arg att acc t ca I e Thr Ser gt c at c cca Val Ile Pro 675 gga gtt ctt G y Val Leu 690 ttg ttc tac Leu Phe Tyr 705 aat cag aaa Asn G n Lys tct Ser caa G n 500 at t l Ie gt a Val cat Hi s at t SIle gt c Val 580 ctt Leu ct c Leu ttc Phe ctt Leu gct Al a 660 at t SIle at t SIle gac Asp t ac Tyr ggt G y 485 gca Al a cgc Ar g ggc G y gac Asp ct c Leu 565 acc Thr ttc Phe t ac Tyr tta Leu gga G y 645 gga G y ct a Leu gat Asp at c SIe cag G n 725 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 Lys Leu Ser aag cag cct cag Lys n Pro G n tgacacacca aaattcgcct ctc 2313 Page 242 seql i st cor r ect ed. t xt <210> 218 <211> 730 <212> PRT <213> Corynebacter i um gl ut arri cum <400> 218 Met Ser Thr 1 Arg Leu IVet Val Gy Al a Ser Ser Asn Sn Val n Al a I I e Val Thr G n Leu Ser Asp G u 115 G n Val Phe 130 G u Lys Leu 145 Thr Tyr Val Phe Al a G y Leu Val I le 195 Val Leu Al a 210 Trp Trp Leu 225 G y Ile Leu Leu Tyr Val G y Ile Al a 275 Al a Ser G y 290 Asp Leu Lys 305 Ser Ile 5 Arg Ile G y Val Ser G n G u G n Val Vbet Asn u 100 Val Ser Val Pr o Ser G u Thr G y 165 Ile Asp 180 Leu Val Thr Ser Al a Lys Phe Il e 245 Al a Ar g 260 Thr G y Ser Thr Ser Asn Thr G u Asn Lys Lys 10 Leu Pro Ala Leu Leu 25 Sy Pro Tyr Phe y 40 Thr Tyr Leu Pro G u 55 G y Asp Phe Thr Asp Ser Asp G u Pro Leu 90 Val Ala y Leu Ser 105 Ala lie Pro Ser u 120 Asn Pro Ser Ala G u 135 Leu Thr G n G n Thr 155 Al a y Phe Thr Al a 170 Leu Leu Leu Ala Val 185 Val Tyr Arg Ser Phe 200 Phe Ala Leu Thr Val 215 Asp Ile Leu Leu Leu 235 Val Ile Gy Ala Al a 250 Arg G u G u Leu Arg 265 Ala lie Arg Ala Ser 280 ile Al a G y Leu Leu 295 Thr Leu Gy Pro Val 315 Lys SIle Lys Ser Ser Thr G u Asp Leu 140 Pr o Asp Al a SIle Al a 220 Ser Thr Val Val Cys 300 Ser Leu Val Al a G u G n Leu Gy 125 Thr Asp Leu Leu Leu 205 Leu Gy Asp G n G u 285 Leu Sy Val Ser Asp Ser G n Asp 110 Ar g G u Tyr Ser Al a 190 Pr o Leu G n Tyr G n 270 Pr o Leu Pr o Tr p G u Al a SIe Asp I e Al a Ser Val Al a 175 Al a SIe Val Thr Ser 255 Asp SIe Phe Ala Ser Val Oy Ile 320 Page 243 seql i st cor r ect ed. t xt Ile Phe Ala IVet Leu Ser Ala Leu Thr Leu Leu Pro Al 325 330 a Leu Leu Phe 335 Val G u Lys Thr 385 Al a Ar g Ser Asp Al a 465 Pr o Val G n Ser Al a 545 I le Leu Al a Al a Tyr 625 Gy SIle Val Gy Al a 355 Al a SIle Gy Gy Al a 435 Val Ser Gy Leu Al a 515 Val l Ie Val Val Leu 595 Pr o SIle Ar g Ser Pr o 675 Val Al a Leu Leu Ser 405 G n l Ie Asn Ser Gy 485 Al a Ar g Gy Asp Leu 565 Thr Phe Tyr Leu Gy 645 Gy Leu Al a Lys Val Leu 390 G n Al a SIle Asn Gy 470 Thr Thr Ser Gy Ar g 550 let Thr Asn Gy Val 630 I le Val Phe Phe Asn G u 375 Leu Ser Leu Val Asp 455 Ser Al a Leu SIle Val 535 Asn Leu Val Hi s Phe 615 Thr Leu Val Leu Pr o 345 I le Hi s Al a Leu G u 425 G u Phe Pr o Gy G u 505 G n Al a SIle Leu Ser 585 Phe Phe SIle Gy Al a 665 G n Lys Pr o Pr o Al a Val 410 Hi s Thr Su I e Pr o 490 Al a Thr Thr SIle Ar g 570 Phe Ser Leu Ar g Leu 650 Al a Ar g Al a Ar g Phe 395 Leu Phe G n Thr Thr 475 Val Pr o Phe Ser Pr o 555 Ser Al a Phe Val G u 635 Thr Thr Pr o Ser Al a 380 Val Gy Pr o Al a Val 460 Al a Val Asp Al a Val 540 I le SIle Thr Pr o Al a 620 G u Val Phe Lys Gy 365 l le Pr o Ser Gy Al a 445 Thr Asp Val Ser Asp 525 Asp Val Val Al a Gy 605 Leu Thr Thr Al a Tyr 350 I le Tr p Thr Ser Gy 430 G n Val Sy G u G u 510 G u Thr Leu Al a Leu 590 Al a Sy Lys Sy Al a 670 G u Tr p Val Leu G u 415 Ser Al a Thr SIe Sy 495 G u Asn Asn Leu Pr o 575 Sy Asp SIe Thr Sy 655 Leu lie Ala Phe lie Val Ala Phe 685 Page 244 Gy Val Leu Ile Asp 690 Leu Phe Tyr Asp II e 705 Asn G n Lys Tyr G n 725 seql i st cor r ect ed. t xt Thr Leu Leu Val Arg Ala Phe Leu Val Pro Ala 695 700 G y Pro Lys lie Trp Trp Pro Ser Lys Leu Ser 710 715 720 Lys G n Pro G n Leu 730 <210> 219 <211> 983 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> RXN03124 <400> 219 at g act cct bet Thr Pro 1 t ac gcg ct a Tyr Al a Leu act ggc gct Thr G y Al a acc atg ccg Thr IVet Pro act gcg ggt Thr Al a G y ctg gtt gct Leu Val Al a att gct gcc I e Al a Al a ttc ctc cca Phe Leu Pro 115 cgc gtg cct Arg Val Pro 130 atg ggt ctg IVet G y Leu 145 ctg ctg gtt Leu Leu Val aat at g cgc Asn IVet Arg ct g Leu 5 at c SIle gat Asp gct Al a gcg Al a tcg Ser at c l Ie ct g Leu cct Pr o tgg Tr p gt c Val 165 gcc Al a gcg Al a gt g Val ct g Leu gct Al a gtt Val 70 at c SIle acc Thr ctt Leu aag Lys gt c Val 150 gtt Val at g Met tcg Ser t cc Ser gag Gu cgt Ar g 55 gt a Val at t SIle gtt Val ggc Gy gtt Val 135 cgc Ar g ttg Leu ccg Pr o at g Met cgt Ar g cca Pr o 40 gcg Al a ttc Phe aat Asn gcc Al a ct g Leu 120 ccg Pr o ctt Leu ctt Leu act Thr att ggt ie G y 10 ttc cgc Phe Ar g aag gaa Lys G u cat gcg Hi s Al a gcg ggt Al a G y att cca Ile Pro 90 atc gca I e Al a 105 ctt ggc Leu G y gat cct Asp Pro gtg cgc Val Arg ggt gca G y Al a 170 gat ggc Asp y ct g Leu aat Asn ttg Leu at g Met acc Thr ttt Phe gtt Val act Thr gag G u aag Lys 155 at c l Ie gct Al a gag Gu gct Al a gga Gy acg Thr ct a Leu ct g Leu cgc Ar g gac Asp 140 at g Met gca Al a gt c Val ttg Leu gag Gu at g Met gt g Val acc Thr gtt Val at c SIle 125 gag G u ccg Pr o at t l Ie ggt Gy at t I e cgt Ar g gct Al a ct g Leu gt g Val gct Al a 110 ttc Phe aag Lys gt g Val cct Pr o at c I e tct Ser ctg Leu gtg Val at c SIe at g Met ctg Leu gca Al a cca Pr o gct Al a gcg Al a 175 acc tcc acg ctg ggc acc Thr Ser Thr Leu G y Thr Page 245 IND seql i st cor r ect ed. t xt 180 185 190 Sgcg ccg cgc acg ggg tat gac atg acg gca gat gcg ttc ggc ccg ggc 624 Al a Pro Arg Thr G y Tyr Asp IVet Thr Al a Asp Al a Phe G y Pro G y 195 200 205 d) 4 cgc aac gcg ccc atg att gcg ctt atc gac gca acc gac gtc cct gag 672 Arg Asn Ala Pro IVet Ile Ala Leu Ile Asp Ala Thr Asp Val Pro G u 210 215 220 gaa gaa cgc cca ttg gtg ttt gga cag gcg gtg gag caa ttc ttg aac 720 G u G u Arg Pro Leu Val Phe Gy G n Ala Val G u G n Phe Leu Asn 225 230 235 240 00 act gat ggt gtg aag aat gct cag atc act cag acc acg gag aat t tc 768 0 Thr Asp G y Val Lys Asn Ala G n Ile Thr G n Thr Thr G u Asn Phe 245 250 255 gat acc gcg cag atc ctg tta ccc cag aat ttg atg cga tcg atg agc 816 SAsp Thr Ala n lie Leu Leu Pro G n Asn Leu IVet Arg Ser IVet Ser 260 265 270 gca cct ctg aga ctc tcg caa ctc ttc gtg cag atg ctg aga cct tcg 864 Ala Pro Leu Arg Leu Ser G n Leu Phe Val G n IVet Leu Arg Pro Ser 275 280 285 ctg atg aca ccg gcg cga cgt atg gca tta ctg gcg tca ccc caa ttt 912 Leu IVet Thr Pro Ala Arg Arg IVet Ala Leu Leu Ala Ser Pro G n Phe 290 295 300 acg atg aca tct ctg ctc gcc tcg gcg acg tcc tgg ttc ctt acg ttc 960 Thr IVet Thr Ser Leu Leu Ala Ser Ala Thr Ser Trp Phe Leu Thr Phe 305 310 315 320 tgatcgtttt ggttctagcg ttc 983 <210> 220 <211> 320 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 220 IVet Thr Pro Thr Leu Ala Ser IVet I e y Leu Ala Val y I e Asp 1 5 10 Tyr Ala Leu Phe lie Val Ser Arg Phe Arg Asn G u Leu Ile Ser G n 25 Thr y Ala Asn Asp Leu G u Pro Lys G u Leu Ala G u Arg Leu Arg 40 Thr IVet Pro Leu Al a Al a Arg Al a Hi s Al a IVet G y Iet Al a Val G y 55 Thr Ala y Ser Ala Val Val Phe Ala y Thr Thr Val Leu Ile Ala 70 75 Leu Val Ala Leu Ser Ile lie Asn lie Pro Phe Leu Thr Val IVet Ala 90 Ile Ala Ala Ala lle Thr Val Ala lle Ala Val Leu Val Ala Leu Ser 100 105 110 Phe Leu Pro Ala Leu Leu y Leu Leu y Thr Arg lie Phe Ala Ala 115 120 125 Arg Val Pro y Pro Lys Val Pro Asp Pro G u Asp G u Lys Pro Thr Page 246 seql i st cor r ect ed. t xt 135 Arg Leu Val Met 145 Leu Asn Al a Ar g G u 225 Thr Asp Al a Leu Thr 305 Leu Val Ar g Ar g 195 Al a Ar g Gy Al a Leu 275 Thr Thr Tr p Val 165 Al a Gy et Leu Lys 245 l le Leu Al a Leu Leu Pr o Asp Al a 215 Phe Al a Leu G n Ar g 295 Al a Gy Asp 185 Thr l Ie G n l Ie G n 265 Phe Al a Al a 140 Arg Lys IVet 155 Al a I e Al a 170 G y Thr Ser Al a Asp Al a Asp Al a Thr 220 Ala Val u 235 Thr G n Thr 250 Asn Leu IVet Val G n let Leu Leu Al a 300 Thr Ser Tr p 315 Pro Val Al a l Ie Thr Phe 205 Asp G n Thr Ar g Leu 285 Ser Phe Al a 175 Sy Pr o Pr o Leu Asn 255 bet Pr o G n Thr <210> 221 <211> 762 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA01180 <400> 221 atg act cct acc ctg gcg tcg atg att IVet Thr Pro Thr Leu Ala Ser IVet IIe 1 5 tac gcg cta ttt atc gtg tcc cgt ttc Tyr Ala Leu Phe lie Val Ser Arg Phe 25 act ggc gct aat gat ctg gag cca aag Thr y Ala Asn Asp Leu G u Pro Lys 40 acc atg ccg ttg gct gct cgt gcg cat Thr IVet Pro Leu Ala Ala Arg Ala Hi s 55 act gcg ggt tct gcg gtt gta ttc gcg Thr Ala y Ser Ala Val Val Phe Al a ggt ctg gct G y Leu Al a 10 cgc aat gag Arg Asn u gaa ttg gct G u Leu Al a gcg atg gga Al a e t y ggt acc acg G y Thr Thr Page 247 gt c Val ttg Leu gag G u at g Met gt g Val ggt Sy at t I e cgt Ar g gct Al a ct g Leu at c I e tct Ser ctg Leu gtg Val at c SIe seql i st cor r ect ed. t xt ct g Leu at t SIle ttc Phe cgc Ar g at g Met 145 ct g Leu aat Asn gcg Al a cgc Ar g gaa G u 225 act Thr gct Al a gcc Al a cca Pr o 115 cct Pr o ct g Leu gtt Val cgc Ar g cgc Ar g 195 gcg Al a cgc Ar g ggt Gy tcg Ser at c l Ie ct g Leu cct Pr o tgg Tr p gt c Val 165 gcc Al a ggg Gy at g Met ttg Leu aag Lys 245 at t SIle gtt Val ggc Gy gtt Val 135 cgc Ar g ttg Leu ccg Pr o gac Asp gcg Al a 215 ttt Phe gct Al a aat Asn gcc Al a ct g Leu 120 ccg Pr o ctt Leu ctt Leu act Thr at g Met 200 ctt Leu gga Gy cag G n at t SIle at c l Ie 105 ctt Leu gat Asp gt g Val ggt Gy gat Asp 185 acg Thr at c SIle cag G n at c SIle cca Pr o 90 gca Al a ggc Gy cct Pr o cgc Ar g gca Al a 170 ggc Gy gca Al a gac Asp gcg Al a act Thr 250 ttt cta acc Phe Leu Thr gtt ctg gtt Val Leu Val act cgc at c Thr Arg Ile 125 gag gac gag Su Asp u 140 aag atg ccg Lys IVet Pro 155 atc gca at t I e Al a I le acc tcc acg Thr Ser Thr gat gcg ttc Asp Al a Phe 205 gca acc gac Al a Thr Asp 220 gtg gag caa Val u n 235 cag acc acg G n Thr Thr gt g Val gct Al a 110 ttc Phe aag Lys gt g Val cct Pr o ct g Leu 190 ggc Gy gt c Val ttc Phe gag G u at g Met ctg Leu gca Al a cca Pr o gct Al a gcg Al a 175 ggc Gy ccg Pr o cct Pr o ttg Leu <210> 222 <211> 254 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 222 IVet Thr Pro Thr Leu Ala Ser IVet lie 1 5 Tyr Ala Leu Phe lie Val Ser Arg Phe 25 Thr y Ala Asn Asp Leu G u Pro Lys 40 Thr IVet Pro Leu Ala Ala Arg Ala His 55 Thr Ala y Ser Ala Val Val Phe Ala 70 Gy Leu Ala Val Gy Ile Asp 10 Arg Asn u Leu Ile Ser n G u Leu Ala G u Arg Leu Arg Ala vebt Gy vet Ala Val y Sy Thr Thr Val Leu Ile Ala 75 Page 248 seql i st cor r ect ed. t xt Leu Val Ala Leu Ser Ile lie Asn lie Pro Phe Leu Thr 90 Val MVt Ala SIle Phe Ar g Met 145 Leu Asn Al a Ar g G u 225 Thr Al a Pr o 115 Pr o Leu Val Ar g Ar g 195 Al a Ar g Gy l Ie Leu Pr o Tr p Val 165 Al a Gy Met Leu Lys 245 Val Gy Val 135 Ar g Leu Pr o Asp Al a 215 Phe Al a Al a Leu 120 Pr o Leu Leu Thr Met 200 Leu Gy G n l Ie 105 Leu Asp Val Gy Asp 185 Thr SIle G n lie Al a Gy Pr o Ar g Al a 170 Gy Al a Asp Al a Thr 250 Val Thr G u Lys 155 l le Thr Asp Al a Val 235 G n Leu Ar g Asp 140 Met Al a Ser Al a Thr 220 G u Thr Val SIle 125 G u Pr o l Ie Thr Phe 205 Asp G n Thr Al a 110 Phe Lys Val Pr o Leu 190 Gy Val Phe G u Leu Al a Pr o Al a Al a 175 Gy Pr o Pro Leu <210> 223 <211> 393 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(370) <223> RXA02586 <400> 223 ttctctgaga tcgtcatgat gaagtacatc gcgt t cggca gatgccacca tcatccgcat gctgct t gtc ccccgccgt g gac Asp at g Met cgc Ar g ggc gty gat aac Asn cac Hi s aac Asn acc Thr gt g tgg Tr p tct Ser gat Asp gca Al a ttc gca Al a gag G u gag G u tct Ser gt g ccc Pr o gt g Val gaa G u cgc Ar g gaa ttc Phe gaa G u acc Thr ggt agy aag gtt aaa Val Lys 15 gca cct Ala Pro gtg cat Val Hi s ttg agc Leu Ser gct cgt Page 249 tgatcgcagc gctgat t ctg atg cac ctg ctt cgc IVet His Leu Leu Arg 1 aag gcc tac acc gtc Lys Ala Tyr Thr Val cgc cca acc acc cgt Arg Pro Thr Thr Arg gaa gca gtt gtc gct G u Ala Val Val Ala acg cag gaa aac cgt Thr n u Asn Arg ttg gaa aag cgc agg seql i st cor r ect ed. t xt Asp Leu Val Ser Phe Val G u Leu Lys Ala Arg Leu G u Lys Arg Arg 75 80 ctt gag gat cta gat taaatctatg cgaggatttt tca Leu G u Asp Leu Asp <210> 224 <211> <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 224 lIet His Leu Leu Arg Asp Asp Asn Trp 1 5 Lys Al a Tyr Thr Val Mbt G y Hi s G y 25 Arg Pro Thr Thr Arg Arg Leu Asn Asp 40 G u Ala Val Val Ala G y Asp Thr Val 55 Thr n G u Asn Arg Asp Leu Val Ser 70 Leu G u Lys Arg Arg Leu G u Asp Leu Trp Ala Pro Gy Phe Val Lys 10 Ser u Val Gu Gu Ala Pro Asp G u G u Val Thr Val His Ala Ser Arg Gy y Leu Ser Phe Val G u Leu Lys Ala Arg 75 Asp <210> 225 <211> 2214 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(2191) <223> RXA02587 <400> 225 agcctggata acctgccaga cggtggcgca tggctgcagc ttgttatcca accgccacaa ttcccaggag taatccaccc ggc Gy gcc Al a cgc Ar g gcg Al a gt c Val ttt Phe at t l Ie agc Ser cgc Ar g ttg Leu t ac Tyr gct Al a gaa G u gag G u ttt Phe aga Ar g tt g Leu gga Gy tt g Leu act Thr ttt Phe ttt Phe tgg Tr p gag G u gcg Al a cgc Ar g at t SIle gat Asp ttt Phe gaa Gu att gtt Ile Val 15 ttc ggc Phe G y cct ggt Pro G y ggg cgt G y Arg ggc act G y Thr Page cgt t ccgccc tctgactgcc gtg ttt tct aaa tgg Val Phe Ser Lys Trp 1 ccg tta gtc gtc atc Pro Leu Val Val lie acc aag ctg ggc gac Thr Lys Leu G y Asp tct tcc tcg acc gct Ser Ser Ser Thr Al a gac aat gac ggc gat Asp Asn Asp Gy Asp tct ttc gat gat gca Ser Phe Asp Asp Al a seql i st cor r ect ed. t xt gag G u aac Asn caa G n ct c Leu gat Asp 150 gcg Al a gat Asp ct g Leu gt g Val gct Al a 230 ct g Leu ttc Phe acc Thr gt g Val aag Lys 310 at g Met at c SIle ttc Phe gat Asp ct c Leu 120 ggt Gy ct c Leu gcc Al a t ca Ser at c SIle 200 at t SIle ttc Phe ttg Leu gag G u acg Thr 280 gt g Val gt g Val gt g Val aag Lys agc Ser gt c Val agc Ser ggc Gy ccg Pr o gct Al a 170 gcc Al a ttt Phe t cc Ser cag G n ctt Leu 250 at g Met acc Thr ct g Leu ttc Phe gt g Val 330 agt Ser at c SIle agc Ser aaa Lys gag G u gac Asp 155 gt a Val gaa G u ggc Gy at c SIle gt c Val 235 gcc Al a gat Asp gcg Al a t cc Ser ggt Gy 315 tt g Leu tt g Leu tct Ser cac Hi s gac Asp caa G n 140 aac Asn gcc Al a gtt Val t ca Ser ttg Leu 220 aac Asn at t l Ie aag Lys ggt Gy ggg Gy 300 gcg Al a ccg Pr o cgt Ar g tac tta gat ggg Tyr Leu Asp y 95 aac agc tac ttt Asn Ser Tyr Phe 110 acc caa acc ttt Thr G n Thr Phe ctg aag gac ttc Leu Lys Asp Phe 145 gcc ggt ggc gtc Al a G y y Val 160 gca ctc gat gag Ala Leu Asp G u 175 gcg ctg cct ttc Ala Leu Pro Phe 190 gtt gcc gcg gcg Val Ala Ala Ala tcg ctg ggc at c Ser Leu Gy Ile 225 ttt gca caa tct Phe Ala G n Ser 240 tat ggc tta ttc Tyr G y Leu Phe 255 acc ccg gtt gaa Thr Pro Val G u 270 act gtg gtg ttc Thr Val Val Phe ttt gtt ttc cca Phe Val Phe Pro 305 tcc gcg gtt ggc Ser Ala Val y 320 ctg ttc agc atg Leu Phe Ser IVet 335 act gct cga aca Thr Ala Arg Thr Page 251 ct a Leu gac Asp gca Al a 130 cgg Ar g acc Thr ggc Gy gt g Val at g Met 210 ttg Leu gtt Val at g Met cag G n tct Ser 290 cag G n ctt Leu ttg Leu at c SIle act Thr 115 gct Al a gag G u act Thr at g Met gct Al a 195 cca Pr o gca Al a gt g Val gt c Val gct Al a 275 gca Al a gct Al a gct Al a ggt Gy gag G u 100 cgt Ar g ct c Leu at t SIle gag G u gct Al a 180 at c l Ie ttg Leu at t l Ie acc Thr tct Ser 260 gtt Val gcg Al a ttc Phe gct Al a aag Lys 340 aac Asn aat Asn ggg Gy gaa G u gt c Val 165 ggc Gy ttg Leu at c SIe ttg Leu ctt Leu 245 cgt Ar g gcc Al a at g Met ttg Leu ttg Leu 325 aat Asn 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 gcg cgc cgt ttg Ala Arg Arg Leu seql i st cor r ect ed. t xt 350 gaa G u aag Lys ccg Pr o 390 cca Pr o ccc Pr o aac Asn ct g Leu gt g Val 470 gag G u ggt Gy aag Lys at g Met at g Met 550 ttc Phe ct g Leu t cc Ser gat Asp acc Thr 360 gt g Val acg Thr aac Asn ttc Phe cag G n 440 gat Asp tct Ser ctt Leu act Thr ct c Leu 520 ttg Leu at t SIle gat Asp agt Ser gac Asp 600 ggc Gy tgg Tr p gt g Val gt g Val acc Thr 410 acc Thr at c SIle ttc Phe ggt Gy gct Al a 490 gcc Al a at g Met tt t ttt Phe ggt Gy gt g Val 570 gt g Val gag G u t cc Ser cgc Ar g gt c Val 380 ttc Phe gt c Val ccg Pr o at c SIle gca Al a 460 cag G n t cc Ser gag G u ct c Leu tcg Ser 540 ggt Gy gcc Al a gt g Val ttc Phe gac Asp gt g Val 365 gt a Val ggc Gy gcc Al a gt c Val t at Tyr 445 ggt Gy gat Asp gt c Val at c SIle t ac Tyr 525 gt g Val gcc Al a agc Ser ct g Leu ct g Leu 605 gac Asp ccg gca tgg gca Pro Ala Trp Al a ttg ctc ttg ctt Leu Leu Leu Leu 385 ggc atc aat gaa G y Ile Asn G u 400 caa gag cgt ttc Sn G u Arg Phe 415 aag ctt gtg gtc Lys Leu Val Val 430 gtt cag gcc aac Val n Ala Asn gcg act acc gat Ala Thr Thr Asp 465 cgt tcc ctc aat Arg Ser Leu Asn 480 cct gag ggc gtt Pro G u G y Val 495 gaa tcc att gag Su Ser Ile u 510 att gtg ctg gcc Ile Val Leu Ala att ttg ccg gcg Ile Leu Pro Ala 545 acc ttg ggt att Thr Leu Gy Ile 560 gca ttg aac ttc Ala Leu Asn Phe 575 atc atg gct att Ile IVet Ala lle 590 gta tct cgc atg Val Ser Arg IVet gcc atc aga tac Ala lie Arg Tyr Page 252 at g Met 370 gct Al a acg Thr gac Asp acc Thr gaa G u 450 gat Asp gag G u gag G u gcg Al a act Thr 530 aag Lys ct c Leu t cc Ser at t SIle gt g Val 610 cgc Ar g ctt Leu t at Tyr gag G u ggg Gy 435 gtt Val ggc Gy cag G n gt g Val ct c Leu 515 ttc Phe gcc Al a acc Thr cct Pr o t ac Tyr 595 gag G u cat Hi s aca Thr ct g Leu gcg Al a 420 gcg Al a gag G u acc Thr gt a Val cag G n 500 ttt Phe at c SIe at c SIe ttg Leu ggc Sy 580 gga Sy gcc Al a 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 ggc act gca cac G y Thr Ala His seqI i st car r ect ed. t xt 615 620 625 acc gga tct at cat cacc gcg gcc gca ctg at cat gat t Thr G31y Ser I Ie I Ie Thr Al a Ala Ala Leu Ilie N/b t I Ie 630 635 640 gga gcg t tt ggt t t ctct gag at cgt cat gat gaag tac G31y Al a Phe G31y Phe Ser O31u I Ie Val I'b t I'b t Lys Tyr 650 655 ggc at g at c gca gcg ct g at t ct g gat gcc acc at c at c G31y 'b t Ile Ala Ala Leu Ilie Leu Asp AiaThr Ilie Ilie 665 670 ct t gtc CCCC cgc cgt gat gca cct gct t cg cga cga caa Leu Val Pr o Ar g Ar g Asp Al a Pr o Al a Ser Ar g Ar g O31n 680 685 690 acc cgg ct t cgt t aaaaaggcc t acaccgtca tgg Thr Ar g Leu Ar g 695 <210> 226 <211> 697 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn gt g gt c t gt Val Val Cys 645 at c gcg tt c I I e Al a Phe 660 cgc at g ct g Ar g I'b t Leu 675 ct g gt g ggc Leu Val G31y 2035 2083 2131 2179 2214 <400> 226 Val Phe Ser Pr o Thr Ser As p Ser G31y Ph e Ph e Ph e 145 ValI O31u Ph e ValI Le u Ser As p As p I Ie Thr 115 Al a O31u Thr vb t Al a 1 95 Lys Tr p Val I Ie 31y As p Thr Al a 31y As p As p Al a 31u As n 1 00 Ar g As n Leu 03 y I Ie O31u 03 u Val 1 65 Al a 03 y 1 80 1 1 e Leu Al a Ar g Al a ValI 70 O31u As n O31n Le u As p 150 Al a As p Le u Al a vb t Al a ValI ValI Pr o As n Ly s 135 O31n G31y I Ie Le u 0 y Hi s Phe Al a I Ie Ser 40 Ar g Le u Ph e As p Le u 120 G31y Le u Al a Ser I Ie 200 Le u 25 O31n I Ie Le u Ser O31u 1 05 Le u As p Hi s Thr Ar g 1 85 ValI Tyr 10 Al a O31u O31u Ph e Ser 90 ValI Ser O31y Pr o Al a 1 70 Al a Ar g Le u O31y Le u Thr li1e Ser Ly s O31u As p 1 ValI (31u Ph e Ph e Tr p O31u Al a Ser Hi s As p O31n 140 As n Al a ValI Ar g ValI As p Thr Pr o O31y I Ie O31y 125 Thr Le u As p Ph e Ar g I Ie As p Ph e O31u Tyr As n 110 Thr Le u Al a Al a Al a 1 lIe Ph e Pr a O31y O31y Le u Ser O31n Ly s O31y Le u 1 Le u Phe O31y Ser Val Val Al a Al a 205 Page 253 Ala I'bt Pro Leu lieVal 210 seqI i st car r ect ed. t xt Gy Ilie Leu Ser Ilie Leu G1y 215 220 Ser Leu G31y I Ie 225 Ser Ph e O31u Ph e Pr o 305 G31y vb t Thr Al a Le u 385 O31u Ph e ValI As n As p 465 As n ValI O31u Al a Al a 545 li1e Al a ValI ValI Al a 275 Al a Al a Al a G31y Ar g 355 Ar g Le u Tyr O31u G31y 435 ValI G31y O31n ValI Le u 515 Ph e Al a Thr Le u Le u 245 Ar g Al a vb t Le u Le u 325 As n Le u Al a ValI Pr o 405 Ph e As p G31y Thr ValI 485 (31u Le u 565 Al a 230 Le u Ph e Thr ValI Ly s 310 vb t I Ie (31u Ly s Pr o 390 Pr o Pr a As n Le u ValI 470 (31u G31y Ly s vb t vb t 550 Ph e G31y G31y Ar g Thr Al a 295 Ser Ser As p As p Al a 375 Le u Al a Al a As n Thr 455 Le u O31n G31y Le u Al a 535 Thr ValI Ph e G31y O31u 265 Al a Al a Al a Thr Tr p 345 li1e Thr G31y As p Ar g 425 Le u Ar g Thr Ar g Pr a 505 Tr p ValI Le u (31y O31n Le u 250 vb t Thr Le u Ph e ValI 330 Ser Tr p ValI ValI Thr 410 Thr I Ie Ph e G31y Al a 490 Al a vb t Ph e G31y ValI 235 Al a As p Al a Ser G31y 315 Le u Le u Tyr G31y Ly s 395 Ar g O31u As p Thr I Ie 475 li1e vb t Al a G31y vb t 555 ValI As p G31y Ly s 285 Le u I Ie Ser Ar g ValI 365 ValI G31y Al a ValI Tyr 445 O31y As p ValI I Ie Tyr 525 ValI Al a Al a G31y 255 Pr a ValI ValI Al a Ph e 335 Al a Al a Le u I Ie O31u 415 Le u O31n Thr Ser O31u 495 Se r ValI Le u Le u Val (3y Al a Ser Al a Leu Asn 570 575 Page 254 seqI i st car r ec Phe Ser Pro G1y Pro Leu I'bt Ser Pr o Val Leu 580 585 1 1 e I I e Ty r G3 y Leu Ser Thr As p Ty r (3 u Val1 595 600 I'b t Val O31u Al a Ar g As p Lys O31y O31u Ser Thr 610 615 Tyr O31y Thr Al a Hi s Thr O31y Ser I Ie I Ie Thr 625 630 635 I'b t Ilie Val Val Cys O31y Al a Phe O31y Phe Ser 645 650 Lys Tyr Ilie Al a Phe 31y 'b t Ilie Al a Al a Leu 660 665 Ilie Ilie Ar g I'b t Leu Leu Val Pr o Ar g Ar g Asp 675 680 Ar g O31n Leu Val G31y Thr Ar g Leu Ar g 690 695 <210> 227 <211> 729 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (706) <223> RXN03042 <400> 227 atgacaccgg cgcgacgtat ggcattactg gcgtacccca ted. t xt Val Leu Phe Leu 605 As p As p 620 Al a Al a (31u I Ie I I e Leu Al a Pr o 685 I Ie 590 ValI Al a Al a ValI As p 670 Al a vb t Ser I Ie Le u vb t 655 Al a Ser at t tacgat g acat ct ctgc t cgcct cggc gacgt cct gg t t cct t acgt t ct gat cgt t t tg gt t Leu Val ct a gcg tt c Leu Al a Phe ct c Le u gct Al a gcg Al a ct g Le u gcc Al a ac c Thr ct g Le u ggc G31y Ph e t cc Ser g at As p ggc G31y ct c Le u ggc G31y g aa O31u tt g Le u c ag O31n act Thr gt g ValI t t g Le u ggt G31y ccg Pr o at c I Ie 75 gcg Al a tt c Ph e t ca Ser gct Al a at c I Ie tt c Ph e ggc G31y t cc Ser ct g Le u ggc G31y ct c Le u gt t ValI gca Al a at t lIe 15 gct Al a at c I Ie at c I Ie act Thr ac g Thr 95 t gg Tr p ac c Thr at c G31y cgt Ar g t cg Ser cca Pr o ggt G31y g at As p gt a ValI cgt Ar g ggt G31y at c lIe ac c Thr c ag O31n ggt G31y ggc G31y aag 1 00 ggt gcc cgc gt g gt c act gct gcg gcg ct g at c at g gt g t ct gt g tt c Page 255 IND seqI i st car r ect ed. t xt (3 OyAl aAr gVal Val Thr Al aAl aAl aLeuIlIe Ibt Val Ser Val Phe 105 110 115 gcg gca t tc at a gcg cag gac at g gcg t tt at t aag acc at g ggc ttt 499 fl Al a Al a Phe I Ie Al a O31n As p I'b t Al a Phe I Ie Lys Thr I'b t G31y Phe 120 125 130 Sgct ct g gcc gt t gct gt g t tc t tc gat gcc t tc gt t gt t cgc at g at g 547 Al a Leu AliaVal Al aVal Phe Phe As pAl aPhe Val Val Ar g 'b t I'b t 135 140 145 at t at c cct gca aca at g t tc ct g ct t gat gac aag gct t gg t gg ct a 595 I Ie I Ie Pr o Al a Thr I'b t Phe Leu Leu As p As p Lys Al a Tr p Tr p Leu S150 155 160 165 00 Scct aag t gg t tg gat aag at t ct t ccc aac gt t gat gt t gaa ggt gag 643 SPr o Lys Tr p Leu As p Lys I Ie Leu Pr o As n Val As p Val O31u 31y O31u C1170 175 180 Sggt ct t agt gaa ct a cat gag gct cgc acc gag gaa ct g aag gaa aat 691 (3 OyLeu Ser (1u Leu Hi s 1u Al aAr gThr(1u (1u Leu Lys(1u As n C1185 190 195 gt a ggt gt c ggg gct t agagaaaca aaaaaggct g ct a 729 Val G31y Val G31y Al a 200 <210> 228 <211> 202 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 228 Leu Val Leu Al a Phe Leu Val Leu Leu Leu Val Phe Ar g Ser I Ie Tr p 1 5 10 Val Pro Leu lieAla Ala Leu Gy Phe G1y Leu Ser Val Leu Ala Thr 25 Phe (1y Al aTh r Val Ala Ilie Phe(3n (1u (1y Al aPhe (1y I Ie I Ie 40 Asp Asp Pr o O31n Pr o Leu Leu Ser Phe Leu Pro Ilie I'b t Leu I Ie G31y 55 Leu Val Phe G31y Leu Al a I'b t Asp Tyr O31n Ilie Phe Leu Val Thr Ar g 70 75 I'b t Ar g 31u G31y Phe Thr Lys G31y Lys Thr Al a G31y Asn Al a Thr Ser 90 Asn O31y Phe Lys Hi s O31y Al a Ar g Val Val Thr Al a Al a Al a Leu Ilie 100 105 110 I'b t Val Ser Val Phe Al a Al a Phe Ilie Al a O31n Asp I'b t Al a Phe Ilie 115 120 125 Lys Thr I'b t O31y Phe Al a Leu Al a Val Al a Val Phe Phe Asp Al a Phe 130 135 140 Val Val Ar g 'b t 'b t Ilie Ilie Pr o Al a Thr I'b t Phe Leu Leu Asp Asp 145 150 155 160 Lys Al a Tr p Tr p Leu Pr o Lys Tr p Leu Asp Lys Ilie Leu Pr o Asn Val 165 170 175 Asp Val (31u (31y 31u (31y Leu Ser (31u Leu Hi s (31u Al a Ar g Thr (31u Page 256 seql i st cor r ect ed. t xt 180 185 G u Leu Lys G u Asn Val y Val Gy Al a 195 200 <210> 229 <211> 729 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(706) <223> FRXA02893 <400> 229 atgacaccgg cgcgacgtat ggcattactg gcgtacccca atttacgatg acatctctgc tcgcctcggc gacgtcctgg ttcct t acgt t ct gat cgt t gtt cta gcg Val Leu Al a ct c Leu gct Al a gcg Al a ct g Leu gcc Al a acc Thr ggt Gy gcg Al a gct Al a at t l Ie 150 cct Pr o ct g Leu G y ttc Phe t cc Ser gat Asp ggc G y cgc Ar g ttc Phe 120 gcc Al a cct Pr o tgg Tr p ct c Leu ggc G y gaa G u ttg Leu cag G n act Thr gt c Val gcg Al a gct Al a aca Thr gat Asp 170 ttc Phe t ca Ser gct Al a at c I e ttc Phe ggc G y gct Al a gac Asp ttc Phe 140 ttc Phe at t SIle t cc Ser ct g Leu ggc G y ct c Leu gtt Val gca Al a gcg Al a 110 gcg Al a gat Asp ctt Leu ccc Pr o at t I e 15 gct Al a at c SIle at c SIle act Thr acg Thr 95 ct g Leu ttt Phe gcc Al a gat Asp aac Asn 175 tgg Tr p acc Thr at c SIle ggc G y cgt Ar g 80 tcg Ser at c SIle at t SIle ttc Phe gac Asp 160 gtt Val cca Pr o ggt G y gat Asp gt a Val cgt Ar g ggt G y gt g Val acc Thr 130 gtt Val gct Al a gtt Val ttg Leu gct Al a cct Pr o ttt Phe gag G u ttc Phe tct Ser 115 at g Met cgc Ar g tgg Tr p gaa Gu at c I e acc Thr cag G n ggt G y ggc G y aag Lys 100 gt g Val ggc Gy at g let tgg Tr p ggt G1y 180 ttc Phe gcg Al a gtg Val cca Pr o ctg Leu ttc Phe cac Hi s ttc Phe ttt Phe at g let ct a Leu 165 gag G u ggt ctt agt gaa cta cat gag gct cgc acc gag gaa ctg aag gaa aat Page 257 seqi i st car r ect ed. t xt G31y Leu Ser Ou Leu His Ou Ala Arg Thr Ou Ou Leu Lys Ou Asn 185 190 195 gt a ggt gt c ggg gct t agagaaaca aaaaaggct g ct a Val G31y Val G31y Ala 200 <210O> 230 <21 1> 202 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn Le u ValI Ph e As p Le u vb t As n vb t Ly s ValI 145 Ly s As p (31u O> 230 Val Leu A a Le u A a Pr o Ph e (31u Ph e Ser 115 vb t Ar g Tr p (31u Ly s 1 95 Ph e A a ValI Pr o Le u Ph e Hi s Ph e Ph e vb t Le u 1 65 (31u Le u A a A a Le u A a 70 Th r G31y A a A a I Ie 150 Pr o (31y ValI Le u I Ie Le u vb t Ly s A a A a Le u 135 li1e Ly s Le u Le u Ph e O31n Ph e Ty r Ly s ValI 1 05 li1e ValI A a Le u G31u 1 85 Le u 10 G31y O31u Le u O31n Th r 90 ValI A a A a Th r As p 1 70 Le u ValI Le u G31y Pr o I Ie A a Th r O31n ValI vb t 1 Ly s Hi s Ph e Ser A a lIe Ph e G31y A a As p Ph e 140 Ph e I Ie (31u Ar g ValI Ph e vb t Le u As n A a vb t 125 Ph e Le u Le u A a Ser Le u G31y Le u ValI A a A a 110 A a As p Le u Pr a Ar g 1 lIe A a I Ie I Ie Th r Th r Le u Ph e A a As p As n 1 Th r 31u As nVa1(3 GyVal1(3 GyAla 200 <210O> 231 <21 1> 1605 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (1582) <223> RXA01616 <400> 231 cct acgat t t ccgacgt ct t aaat cgccac acagcgccgt ggt ct aaaac accaacaaaa gagt tgt aac t gt accgacc at tcgt taca gt tacgat cc at g act t ca gaa acc 115 Page 258 seql i st cor r ect ed. t xt lVbt Thr Ser G u Thr tta Leu gtt Val t ac Tyr caa G n cgt Ar g ttc Phe ttt Phe at c SIle acg Thr t cc Ser 150 ct g Leu gt g Val at c SIle ct g Leu act Thr 230 at t l Ie gcg Al a agc Ser gca Al a ttg Leu ggt Gy at g Met cca Pr o gcg Al a 120 gag G u gca Al a gaa G u gtt Val aat Asn 200 aca Thr cgc Ar g gcg Al a gcg Al a ggt Gy cct Pr o at c SIle ggc Gy ttg Leu gcg Al a acg Thr gag G u ctt Leu ttc Phe 170 gcg Al a tct Ser gct Al a at g Met ttg Leu 250 acg Thr ttt Phe ct g Leu aac Asn ttg Leu at t SIle gct Al a at g let gag G u 140 gct Al a tgg Tr p at c l Ie cat Hi s ttg Leu 220 ctt Leu agc Ser aaa Lys ct g Leu cgt Ar g 45 gca Al a ggt Gy ttc Phe ctt Leu cct Pr o 125 cgc Ar g gcg Al a ggt G y gct Al a tgg Tr p 205 at c SIle ctt Leu agt Ser acc Thr at c SIle gaa G u t at Tyr gac Asp gga Gy gtt Val 110 gca Al a aac Asn gca Al a tcg Ser acg Thr 190 gat Asp at c SIle ttg Leu cgt Ar g caa G n 15 ggt Gy cag G n ccc Pr o aaa Lys at c SIle 95 gct Al a acc Thr act Thr ggc G y gtt Val 175 ctt Leu ttc Phe acg Thr ggt Gy cag G n 255 cgt Ar g gt a Val ct c Leu ct g Leu aac Asn 80 gct Al a gcg Al a ttg Leu gca Al a ccg Pr o 160 ttc Phe ttt Phe ttg Leu at c SIle gca Al a 240 aag Lys tgg Tr p gac Asp gca Al a ct c Leu ggc G y t ca Ser aga Ar g gct Al a at t l Ie 145 at c SIle ct c Leu gt g Val tcg Ser aag Lys 225 gt c Val aag Lys gct Al a aac Asn gcc Al a at g Met cac Hi s ctt Leu gct Al a ct g Leu 130 ggt G y at t SIle at t SIle gcg Al a tcg Ser 210 gaa G u at c SIle at c SIle ct c Leu at t SIle gaa G u ggc G y cgg Ar g gct Al a 100 ctt Leu cgc Ar g tgg Tr p ggt G y gtt Val 180 gcc Al a t at Tyr gt g Val ttg Leu gag G0u 260 gcc Al a ct c Leu acc Thr ctt Leu at g let gcg Al a ggc G y att SIe ggt G y gcg Al a 165 ccg Pr o aat Asn gcg Al a aat Asn at c SIe 245 cca Pr o ctt cta gat ctg tcg ttg ttc cgt aat cgc ctt ttc tta ggc ggt gtg Page 259 Leu Leu Asp Leu 265 seql i st cor r ect ed. t xt Ser Leu Phe Arg Asn Arg Leu Phe Leu 270 G y G y Val 275 gtt Val acc Thr ggt Gy 310 at c SIle ggt Gy gcg Al a at g let at c SIle 390 gag G u agc Ser gat Asp cgt Ar g gt a Val 470 gcg Al a 280 cag G n ct c Leu ggt Gy ttt Phe cat Hi s 360 gcg Al a t cc Ser tct Ser ttc Phe ttc Phe 440 tct Ser gca Al a at g Met ttc Phe at c SIle aac Asn 330 gcc Al a gat Asp gct Al a ccg Pr o gag G u 410 ttc Phe gcg Al a gac Asp gcg Al a gcg Al a cag G n cca Pr o 315 ct g Leu act Thr ggt Gy ggt G y gt g Val 395 tt c Phe tt c Phe ggt G y acc Thr gct Al a 475 at g Met ttg Leu 300 gct Al a cat Hi s gcc Al a ttg Leu tcg Ser 380 cgt Ar g ggc G y t ac Tyr gtt Val gca Al a 460 gct Al a act Thr gt g Val ttg Leu tgg Tr p ggc Gy 350 ttt Phe at g Met gct Al a ct g Leu ct g Leu 430 cac Hi s at t l Ie at c l Ie gtg tcc Val Ser ggt ttc G y Phe ggt agc G y Ser 320 ggc ttc G y Phe 335 atc gcc I e Al a ttc atc Phe Il e tct gtg Ser Val ggc at g G y ltt 400 ttg tct Leu Ser 415 cat gcc Hi s Al a gcg att Ala I e aac gt g Asn Val agc agt Ser Ser 480 ggt G y act Thr 305 ttc Phe aaa Lys ct g Leu gcg Al a tct Ser 385 gcg Al a gt c Val ccg Pr o gat Asp ttg Leu 465 t ac ttg Leu 290 cca Pr o ccg Pr o ccg Pr o tgt Cys ggt Gy 370 t cc Ser tcg Ser gcg Al a gca Al a ggc G y 450 at c SIle ctt gaa G u ctt Leu at g let ct g Leu at t SIle 355 ct a Leu act Thr tcg Ser at t l Ie gag G u 435 gat Asp at t SIle ttc at g let gag G u tct Ser at c SIle 340 tgg Tr p ttc Phe gcg Al a at c SIle ttg Leu 420 gtt Val gcg Al a gcc Al a cgc act Thr gct Al a at t SIe 325 agt Ser ggc Gy ttc Phe at t SIe gaa G u 405 ggt G y gcg Al a gcg Al a ct a Leu gga 485 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1602 1605 Tyr Leu Phe Arg aat ccg aag gga gcc aat aat gcg Asn Pro Lys Gy Ala Asn Asn Ala 490 tgc <210> 232 <211> 494 <212> PRT <213> Corynebacter i um gl ut ai cur <400> 232 cac tagtaaaaaa gagatgattc Hi s Page 260 seql i st cor r ect ed. t xt Met Thr Ser G u Thr Leu G n Ala G n Ala Pro Thr Lys 1 5 10 Thr G n Arg Tr p Asp Al a Leu Gy Ser Ar g Al a SIle 145 I le Leu Val Ser Lys 225 Val Lys Phe Gy Thr 305 Phe Lys Phe Ser Ser Al a Ar g Gy Phe 115 l e I e Gy Asn Pr o 195 Phe Ser Mbet G u Gy 275 G u Leu Met Leu Al a Leu Thr Leu b t Al a Gy SIle Gy Al a 165 Pr o Asn Al a Asn SIle 245 Pr o Val Thr Al a SI e 325 Ser l Ie Thr Al a Leu Leu Al a Gy Phe 135 Val Leu Al a Al a Leu 215 Al a Sy Leu Al a Ser 295 Leu Gy Gy Ser Al a 40 Leu Gy Mbet Pr o Al a 120 G u Al a G u Val Asn 200 Thr Ar g Al a Asp Al a 280 G n Leu Gy Phe Gy Leu Tr p Al a Gy Thr 105 Al a Asp l Ie Phe SIle 185 Pr o Leu Hi s Val Leu 265 Gy Ar g vbt Al a Al a 345 Gy Pr o SIle Gy Leu 90 Al a Thr G u Leu Phe 170 Al a Ser Al a vbt Leu 250 Ser b t Phe SI e Asn 330 Al a Leu Leu SIle Thr 75 Ser Tr p Met Ar g Gy 155 Tr p Leu Lys Gy Pr o 235 Phe Leu Al a G n Pr o 315 Leu Thr Phe Leu Asn Leu SIle Al a vbt G u 140 Al a Tr p SIle Hi s Leu 220 Leu Ser Phe b t Leu 300 Al a Hi s Al a Leu Ar g Al a Gy Phe Leu Pr o 125 Ar g Al a Gy Al a Tr p 205 I le Leu Ser Ar g Phe 285 Ser Al a Ar g Val I e G u Tyr Asp Gy Val 110 Al a Asn Al a Ser Thr 190 Asp SIle Leu Ar g Asn 270 Thr Val Leu Tr p Gy 350 Gy G n Pr o Lys SIle Al a Thr Thr Gy Val 175 Leu Phe Thr Gy G n 255 Ar g Val Sy Sy Sy 335 I le Val Leu Leu Asn Al a Al a Leu Al a Pr o 160 Phe Phe Leu SIe Al a 240 Lys Leu Ser Phe Ser 320 Phe Al a Leu Cys lie Trp G y Ala Thr His Thr Asp G y Leu Pro Phe Phe IIe 355 365 Page 261 A a Ser 385 A a ValI Pr o As p Le u 465 Tyr Le u Thr Ser I Ie G31u 435 As p I Ie Ph e Ph e I Ie G31u 405 G31y A a A a Le u G31y 485 G31y 375 G31y ValI Le u As n A a 455 cys Pr o seql i st car r ect ed. t xt Al a G y Al a G y Ser Val 380 Ser Ala Pr o Val Ar g Lys 395 Ser Ty r G3 u Phe a3 y Th r 410 Phe Pr o Phe Phe Ty r Ser 425 Phe Ser Ala a31y Val Hi s 440 445 Ser Leu As p Thr A a Ty r 460 Ala Val Ala Ala Ala Leu 475 Lys a3 y A a As n As n A a 490 vb t A a Le u Le u 430 Hi s I Ie I Ie Hi s Ser a31y Le u 415 Hi s A a As n Ser <210O> 233 <21 1> 1500 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (1477) <223> RXA01666 <400> 233 cgacgcgccc ctccaccttt tcagtagcgt cacgggcgcc agt t tgaggg t tt t tgct cc ccat ct t tag gagacacccc aat cct gt at tt t tagcagc tcc acg ttt cat Ser Thr Phe Hi s aaa Lys tt c Ph e ct g Le u t cc Ser at g vb t ct g Le u tt g Le u gcc A a ggc a31y tt t Ph e tt t Ph e t gg Tr p aac As n gt g ValI gt c ValI gga a31y ag c Ser tt t Ph e at g vb t t gg Tr p gga a31y gt t ValI ac c Thr ct g Le u t cc Ser t at Tyr t ac Tyr g at As p ct c Le u g aa a31u gt c ValI t cc Ser ggt a31y aat As n tt t Ph e 80 ggc a31y act Thr ggc a31y at c lIe 50 aag Lys t gt cys ag c Ser gga a31y aac As n 35 gcc A a aag Ly s ct c Leu at c I Ie tt t Ph e 20 gt c ValI gt t ValI t cc Ser agt Ser gga a31y 1 00 ct g Le u gca A a t gt cys gt c ValI gcc A a aat As n acc gcc ct g t gg gt g t t c gt t t ct t tc at c ct c at c gga t ca at c gt g Page 262 Thr Al a Leu seql i st cor r ect ed. t xt Val Phe Val Ser Phe Ile Leu Ile Gy Ser Ile Val 115 gaa G u cct Pr o ggt Gy 150 ttc Phe gt c Val at c SIle aca Thr gga G y 230 ttg Leu at g Met ct g Leu t cc Ser aat Asn 310 tgg Tr p gct Al a cgc Ar g at g Met 120 gct Al a gga Gy ggc Gy ct g Leu caa G n 200 acc Thr at g Met gcg Al a cct Pr o gga G y 280 act Thr ggt Gy ct c Leu gaa G u caa G n 360 aac Asn cgc Ar g tta Leu gaa G u 170 cac Hi s gac Asp gat Asp at c SIle ttc Phe 250 ggc G y acc Thr ttg Leu gct Al a at c SIle 330 gca Al a cgc Ar g at c SIle gac Asp gt c Val 155 at c SIle ct g Leu gca Al a tt g Leu at c SIle 235 aac Asn ct g Leu agc Ser ggc Gy ttt Phe 315 ct g Leu gaa G u gt a Val gca Al a aaa Lys 140 acc Thr acc Thr ct g Leu cag G n gcg Al a 220 ct g Leu aac Asn gaa G u ct c Leu aaa Lys 300 gtt Val ggc Gy caa G n ttt Phe ct g Leu 125 gca Al a agc Ser ct g Leu ccg Pr o cct Pr o 205 at c l Ie agt Ser ct c Leu ctt Leu ggc G y 285 aac Asn ggc Gy at t SIle acc Thr gga G3y 365 110 t cc Ser aac Asn gt c Val tgg Tr p at t SIle 190 act Thr gt g Val gtt Val at c SIle ttc Phe 270 ttc Phe cct Pr o at g Met ttc Phe at c SIle 350 ct a Leu acc Thr ggc Gy at t SIle at c SIle 175 cgc Ar g gtt Val t cc Ser ccg Pr o ggc G y 255 agc Ser at c SIle gt g Val tta Leu at c SIle 335 ctt Leu gcc Al a gt g Val ct g Leu gct Al a 160 tgc Cys gt c Val tct Ser aaa Lys gga G y 240 ggc G y cca Pr o gtt Val cgc Ar g ttt Phe 320 ttc Phe caa G n at g Met gt c Val gt a Val 145 ggt Gy ctt Leu gac Asp gac Asp ggc G y 225 ct g Leu gt g Val cag G n ggt G y acc Thr 305 gcc Al a at g Met cga Ar g gca Al a acg Thr 130 gga G y t cc Ser ggg G y gaa G u gat Asp 210 at c SIle ct c Leu t ac Tyr ct g Leu ggt G y 290 ttg Leu at t l Ie gct Al a gt c Val gt g Val 370 ct g Leu gcc Al a gcc Al a ct c Leu ccg Pr o 195 t ca Ser gac Asp gcg Al a t cc Ser tgg Tr p 275 gct Al a ct g Leu cgc Ar g at c l Ie gt c Val 355 gaa G u ttg Leu gt g Val at c l Ie t ca Ser 180 gaa G u gtt Val ct a Leu ctt Leu gca Al a 260 ggg G y gt g Val ct g Leu gaa G u acc Thr 340 cca Pr o at g Met gtt Val caa G n ggg G y 165 ctt Leu at c SIe ccc Pr o aaa Lys gt g Val 245 ct c Leu ct a Leu at c SIe gtt Val tgg Tr p 325 cca Pr o ttc Phe gca Al a 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 gcc aac ccg ctc tcc aca gtg atc gtg gcg att ttg gcc gaa gcc tac Page 263 seqI i st car r ect ed. t xt Al aAsn Pro Leu Ser Thr Val lleVal AlalIle Leu Ala 375 380 385 ctc att cca tgg atg gct ggc ccc ggc gcg gac acc atc Leu Ilie Pro Trp I'b t Al a (1y Pro G31y AiaAsp Thr Ilie 390 395 400 atc ctc ggc gag ggt aaa gct cgc ggc atg gca ctg atg Ilie Leu G3 y G3 u G3 y Lys Al a Ar g G3 y I'b t Al a Leu I'b t 410 415 t ca ggt gcc at c at g t t g gt t gt c gt g ct g t tg gca tt c Ser a31y Al a Ilie I'bt Leu Val Val Val Leu Leu Al a Phe 425 430 tcc tac cgg aaa ctc agc cag tac tac gcc acc acc agc Ser Tyr Ar g Lys Leu Ser a3 n Tyr Tyr Al a Thr Thr Ser 440 445 450 gcg gga gct gct gag aag t aagt gct ct agaccgt t gt t t g Al a a3 y Al a Al a a3 u Lys 455 <210> 234 <211> 459 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn a31u t gg Tr p tt c Ph e at g vb t 435 c aa a31n Al a ggc a31y ct c Le u 420 t cg Ser g ac As p Tyr gt g ValI 405 gca Al a ag g Ar g at t I Ie 1315 1363 1411 1459 1 500 <400> 234 Val Ser Thr Phe Hi s Lys 1 5 Thr Thr a3 y Phe Leu Phe Thr a31y Asn Val Al a Leu Leu I Ie Al a Val Cys Ser Ar g Lys Lys Ser Val I'b t 70 Ty r Cys Leu Ser Al a Leu Leu Ser I Ie a31y As n Thr 1 00 I Ie a31y Ser I Ie Val a31u 115 Val Thr Leu Leu Val Pr o 130 Val a31yAla Val a31na(1y 145 150 a3 y Ser Al a I I e a3 y Phe 1 65 Leu a3 y Leu Ser Leu Val 1 80 As p (3u Pr o (3u I Ie I Ie 1 95 ValI Ph e Thr I Ie Le u ValI Al a Hi s a31u 135 ValI Le u Al a Thr Le u Al a a31y 40 Ph e Ph e Tr p Le u vb t 120 Al a a31y a31y Le u (31n 200 I Ie ValI li1e Ph e Ser ValI Tr p 1 Ar g a31u Ph e vb t Le u 1 (31u Thr Ph e Ser Thr ValI 75 Tr p Ph e I Ie As p ValI 1 55 li1e Le u I Ie vb t I Ie ValI Thr a31u Ser Le u 125 Al a Ser Le u Pr o As n Le u vb t Hi s ValI As p lie Thr ay le le 1 Ar g ValI Ser a31y As n Ph e a31y Le u ValI Le u Al a 1 Cys ValI Asp Ala On Pro Thr Val Ser 205 Page 264 seql i st cor r ect ed. t xt Asp Asp 210 G y lle 225 Leu Leu Val Tyr G n Leu G y G y 290 Thr Leu 305 Ala I e Mvt Ala Arg Val Al a Val 370 Leu Al a 385 Thr Ile Leu IVet Al a Phe Thr Ser Ser Val Pro Thr Pro Thr Ser 215 Asp Leu Ala lie Val Ser Lys Asp Al a Ser Tr p 275 Al a Leu Ar g l Ie Val 355 G u G u Tr p Phe vbt 435 G n Lys Val 245 Leu Leu l Ie Val Tr p 325 Pr o Phe Al a Tyr Val 405 Al a Ar g SIle Gy 230 Leu vbt Leu Ser Asn 310 Tr p Al a Ar g Al a Leu 390 I le Ser Ser Al a Ser Phe Asp Leu Lys 295 Val Tr p Al a G n Asn 375 I le Leu Gy Tyr Gy 455 Met Al a Pr o Gy 280 Thr Gy Leu G u G n 360 Pr o Pr o Gy Al a Ar g 440 Al a Lys Ser Tyr 265 Leu Gy Val Tyr Al a 345 Gy Leu Tr p G u l Ie 425 Lys Al a SIle Phe 250 Gy Thr Leu Al a SIle 330 Al a Ar g Ser Met Gy 410 Met Leu G u SIle 235 Asn Leu Ser Gy Phe 315 Leu G u Val Thr Al a 395 Lys Leu Ser Lys 220 Leu Asn G u Leu Lys 300 Val Gy G n Phe Val 380 Gy Al a Val G n Ser Leu Leu Gy 285 Asn Gy SIle Thr Gy 365 I le Pr o Ar g Val Tyr 445 Pr o Gy 255 Ser SIe Val Leu SIe 335 Leu Al a Al a Al a bet 415 Leu Al a <210> 235 <211> 1521 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1498) <223> RXA00062 <400> 235 cttcaacata ggcgttgggg ctgactttta aacaggtacc agtagtaccg gcataagcga tcactgttgc gttttcttgc tgccatcaaa aattagtcac atg att tta agc atc 115 IVet Ile Leu Ser Ile 1 gtc ctt ttg ggc tac ttc atg att ctg ctt gac acc tcc atc gtc att 163 Page 265 Val Leu Leu G y acg Thr ct g Leu ct g Leu t ac Tyr tct Ser gga Gy ttc Phe gtt Val gct Al a 150 gcc Al a agc Ser ggt Gy tgg Tr p ttc Phe 230 ccc Pr o cgc Ar g ct a Leu tgg Tr p gga Gy ggt Gy aat Asn gcg Al a gaa Gu 120 ggc G y ctt Leu gt g Val cct Pr o gcc Al a 200 gat Asp tgg Tr p ggg Gy ctt Leu Tyr gcc Al a cag G n cgt Ar g gcg Al a gcg Al a at c l Ie cca Pr o ggt Sy tcg Ser gcc Al a 170 t ca Ser gt g Val ttt Phe ct g Leu ttt Phe 250 gtt Val at c l Ie agt Ser gcc Al a tt g Leu 75 gt c Val gct Al a gct Al a gt g Val tgg Tr p 155 tac Tyr ct c Leu ct c Leu gt g Val cgc Ar g 235 aaa Lys ggc Gy ggc G y t cc Ser ggt Gy ttt Phe ct c Leu cca Pr o cgc Ar g gca Al a 140 cgc Ar g at a SIle gac Asp t ac Tyr ttg Leu 220 cat Hi s aac Asn t cc Ser seql i st cor r ect ed. t xt Phe IVet iIe Leu Leu Asp Thr Ser agt Ser t ac Tyr 45 gat Asp gcg Al a at t SIle gcg Al a ctt Leu 125 gca Al a at c SIle gt g Val at c SIle gca Al a 205 at t SIle gaa Gu cgc Ar g gt g Val gaa Gu aca Thr at c SIle gct Al a gga Gy aca Thr 110 cgt Ar g ggc G y ggc G y cac Hi s ttc Phe 190 at t l Ie t cc Ser t cc Ser agg Ar g at g let 270 ctt Leu tta Leu ttc Phe t ca Ser gca Al a 95 ctt Leu gct Al a ct a Leu ttc Phe aaa Lys 175 gga G y gt c Val ct c Leu t ca Ser cga Ar g 255 t ca Ser ggc G y gt c Val ggc Gy tcg Ser cgc Ar g gcg Al a acc Thr gt g Val ttt Phe 160 gcc Al a gca Al a cgc Ar g gt g Val gcc Al a 240 aac Asn ttc Phe at c SIle ttc Phe cga Ar g ttg Leu gt a Val ttg Leu tct Ser at c SIle 145 at c SIle at t l Ie at t l Ie agc Ser ct g Leu 225 aaa Lys acc Thr ttc Phe Ile Val lie gat Asp ggc Gy aag Lys gca Al a gtt Val at t SIle gct Al a 130 ggc G y aac Asn ccc Pr o acc Thr gcc Al a 210 ggc Gy gaa Gu at c SIle ttc Phe ccc Pr o gca Al a aaa Lys at t l Ie caa G n act Thr 115 t at Tyr ggc G y gt c Val gca Al a t cc Ser 195 gat Asp at c SIle cca Pr o ttg Leu ttt Phe 275 gtg Val ctt Leu gtg Val gcg Al a ggc Gy 100 gag G u ggt G y gta Val ccc Pr o acc Thr 180 acg Thr t ac Tyr gca Al a ctt Leu gcc Al a 260 gcc Al a cag ctg ttc cag gac acc atg gga atg aat gct ctc cag gca ggc ctt Page 266 G3 n Leu Phe G3 n Asp Thr I'bt G3 280 2 seqI i st car r ect ed. t xt y I'b t As n A a L eu G3 n A a (3 y Le u 5 290 gcg tt c A a Phe 295 cca cgg Pr o Ar g 310 ggt tt c a3 y Phe aac acg Asn Thr caa ggc a31na(1y ccg aag Pr o Lys 375 caa atc a31n le 390 gct gt c A a Val cac tt c Hi s Phe ttt gcg Phe A a cgc cca Ar g Pr o 455 at g vb t ct t Le u gcc A a at g vb t tt c Ph e 360 g ac As p ggC a31y at c I Ie gga a31y gt c ValI 440 ac c Th r ct a Le u cga Ar g at g vb t 330 gcg A a tt c Ph e t cc Ser ac c Th r cat Hi s 410 tt g Le u ct g Le u t ct Ser gca A a 315 gt c ValI ct g Le u gga a31y a31y t t c Ph e 395 g at As p ct c Le u ct g Le u ct g Le u 300 ggC a31y at c I Ie at c I Ie cca Pr o gcc A a 380 ggt a31y gca A a t cc Ser aag Ly s c ag a31n t ct Ser at g vb t cca Pr o 350 ac a Th r t ct Ser ggt a31y t ca Ser gt g ValI 430 c ac Hi s tt t Ph e g at As p gca A a 335 at a I Ie gct A a ggc a31y gt g ValI gag a31u 415 ac g Th r g aa a31u gcc A a t cc Ser 320 ggC a31y gt t ValI ct g Le u ct g Le u tt c Ph e 400 at g vb t ct g Le u ac c Th r ag c Ser 305 at g vb t ct c Le u tt g Le u gca A a gt g ValI 385 t cc Ser at c I Ie ac g Th r cga Ar g gcc A a ct c Le u gca A a gt g ValI gt t ValI 370 aat As n t cc Ser ag c Ser ct g Le u aag Ly s 450 gcg A a ac c Th r tt t Ph e gga a31y 355 c aa a31n t cc Ser tt g Le u g ac As p gcc A a 435 agt Ser at g vb t gt c ValI gt a ValI 340 tt t Ph e ggt a31y ct t Le u gct A a cgc Ar g 420 ac c Th r ag c Ser gt g ValI at c I Ie 325 cca Pr o ggc a31y gca A a c ac Hi s gt c ValI 405 gca A a at c I Ie gag a31u 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 508 1521 ct c gt c gac Leu Val Asp 460 gaa aag gca gtt acc tct a31u Lys Ala Val Thr Ser 465 t agt gcgct g cagcatccca gtt <210> 236 <211> 466 <212> PRT <213> Cor ynebact er i urn gi ut anri cumn <400> 236 I'b t I Ie Leu Ser I Ie Val Leu Leu a31y Tyr Phe I'bt I Ie Leu Leu Asp 1 5 10 Thr Ser I Ie Val Ilie Th r a31y Leu Pro Ala I Ie a31y Se r a31u Leu a31y 25 Ilie Asp Pr o Val Hi s Leu Ser Tr p Val a31n Ser Ser Tyr Thr Leu Val 40 Phe a31y Ala Leu Leu Leu Leu a31y Ala Ar g Ala a31y Asp I Ie Phe a31y 55 Page 267 Ar g Leu Val Leu Ser SIle 145 I e I e I e Ser Leu 225 Lys Thr Phe Leu Ser 305 let Leu Leu Al a Val 385 Ser SIle Lys Lys Val Leu seql i st cor r ect ed. t xt I le G y Leu Al a Leu Phe Al 75 a Ala Ser Ser SIle G n Thr 115 Tyr Gy Val Al a Ser 195 Asp SIle Pr o Leu Phe 275 Al a Al a Thr Phe Gy 355 G n Ser Leu Asp Leu Al a Phe Al a Phe SIle 165 Phe Al a Ser Val Leu 245 Ser Thr Leu Val SIle 325 Pr o Gy Al a Hi s Val 405 Al a Ser Gy Phe Val Al a 150 Al a Ser Gy Tr p Phe 230 Pr o Ar g G n Al a Pr o 310 Sy Asn G n Pr o G n 390 Al a Hi s Pr o Al a Pr o Al a 135 Asp Al a Ar g SIle Thr 215 I le Leu Phe Leu Phe 295 Ar g Phe Thr Sy Lys 375 I le Val Phe Asn Al a G u 120 Sy Leu Val Pr o Al a 200 Asp Tr p Sy Leu Phe 280 lbt Leu Al a l t Phe 360 Asp G y lie Gy Al a l Ie 105 Gy I e Leu Leu Sy 185 Al a Pr o Phe Leu Leu 265 G n Pr o Ser l e I e 345 Al a G n Sy Sy Phe 425 Al a 90 I le Pr o Sy Ser Al a 170 Ser Val Phe Leu Phe 250 Val Asp Leu Ar g lbt 330 Al a Phe Ser Thr Hi s 410 Val Al a Al a Val Tr p 155 Tyr Leu Leu Val Ar g 235 Lys Sy Thr Ser Al a 315 Val Leu Sy Sy Phe 395 Asp Leu Pr o Ar g Al a 140 Ar g SIle Asp Tyr Leu 220 Hi s Asn Ser let Leu 300 Sy SI e SIle Pr o Al a 380 G y Al a SIle Al a Leu 125 Al a SIle Val SIle Al a 205 I le G u Ar g Val Sy 285 Leu Val Sy Leu et 365 Val Leu Thr Al a Leu Al a Leu Phe Lys 175 Sy Val Leu Ser Ar g 255 Ser Asn Phe Asp Al a 335 I le Al a Sy Val G u 415 Ar g Al a Thr Val Phe 160 Al a Al a Ar g Val Al a 240 Asn Phe Al a Al a Ser 320 Sy Val Leu Leu Phe 400 let Leu Leu Ser Thr Val Thr Leu 430 Page 268 seql i st cor r ect ed. t xt Thr Leu Ala Thr lie Phe Ala Val Thr Leu Leu Lys Arg His G u Thr 435 440 445 Arg Lys Ser Ser u Arg Pro Thr G n Leu Val Asp G u Lys Ala Val 450 455 460 Thr Ser 465 <210> 237 <211> 1584 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1561) <223> RXA00215 <400> 237 cagtgcaaac tgaccccgca tcctaaaccg cgccagattt ctacctcaaa gaattgaagg ccttttccag gcgccctcgt gcgtgaaaga ataactcaac cag G n gcc Al a at t SIle gt g Val aac Asn cca Pr o gt c Val ggt Gy ggt Gy cgc Ar g 150 ct a Leu ccc Pr o ttg Leu gt c Val gt c Val ctt Leu gt c Val gcc Al a 120 ggc Gy ttt Phe t cc Ser at g abt at t SIle acc Thr tgg Tr p act Thr ggc Gy gac Asp gcc Al a ttt Phe t cc Ser ccc Pr o ggg Gy cca Pr o gt a Val 75 ggc Gy at g Mbt at g Mbt ctt Leu gag 15u 155 gca Al a ttg Leu ttt Phe gcg Al a acc Thr cgt Ar g gtt Val ttc Phe ttg Leu 140 cgc Ar g gca Al a cct Pr o ttc Phe 45 tta Leu tcg Ser ttg Leu at c SIle acg Thr 125 act Thr cgc Ar g ggt Gy gaa G u at g Mbt cag G n gt g Val ggc Gy ttc Phe 110 ttg Leu cca Pr o ggt Gy agt Ser 15 aag Lys at c SI e gca Al a t at Tyr gac Asp 95 aca Thr at t SIle caa G n gca Al a gct Al a caa G n ct g Leu gac Asp ct c Leu 80 aag Lys gt g Val at c SIle acc Thr gct Al a 160 gt g Val 1 gca Al a gct Al a ttg Leu at g abt ct c Leu t ac Tyr agc Ser gct Al a at g abt 145 ctt Leu tct gac aaa aag Ser Asp Lys Lys ccc caa acc aag Pro G n Thr Lys tgg cca gct cta Trp Pro Ala Leu gat caa acc at c Asp n Thr Ile ggc gcg tcc tac G y Al a Ser Tyr act ttc gcg gtg Thr Phe Ala Val ggt ccg aaa aat Sy Pro Lys Asn 100 tct ttg gcc tgt Ser Leu Ala Cys 115 cgt ggc gtt caa Arg y Val n 130 gca aca atc aac Ala Thr Ile Asn gga gtg tgg ggt Sy Val Trp y 165 Page 269 seql i st cor r ect ed. t xt tct aca gct ggc ctt gca tcc cta gca gga ccg atc ctg Ser Thr Ala y Leu Ala Ser Leu Ala y Pro lie Leu at c SIle at c SIle cca Pr o gt g Val 230 ggc Gy ttt Phe gat Asp ggc Gy ct g Leu 310 gaa G u t ca Ser gca Al a at g Met act Thr 390 t cc Ser gt g Val gaa G u gt g Val 200 ct g Leu gt g Val gcg Al a tgg Tr p ct c Leu 280 at c SIle at c SIle ggt Gy ttt Phe ct c Leu 360 at g Met ct c Leu at g Met aat Asn 170 tgg Tr p tcg Ser cga Ar g ttc Phe tgg Tr p 250 at c SIle cca Pr o at c SIle ttg Leu at g Met 330 gga Gy ttt Phe ttc Phe at c SIle gac Asp 410 acc Thr tgg Tr p at c SIle ctt Leu 220 gt g Val tgg Tr p caa G n gag G u gcc Al a 300 ttc Phe gt g Val ct g Leu aca Thr acg Thr 380 aac Asn cca Pr o caa G n caa G n gca Al a 205 gat Asp ttt Phe at c SIle caa G n at t SIle 285 at g Met cag G n cct Pr o gtt Val gt g Val 365 ggt Gy gcc Al a cac Hi s tta Leu tgg Tr p 190 gt a Val ccg Pr o gct Al a at g Met agc Ser 270 ttc Phe gga Gy caa G n caa G n gat Asp 350 gct Al a ct a Leu ttt Phe aag Lys ggt G3y 430 gt c Val at g Met ctt Leu ttc Phe at c SI e 255 agg Ar g aag Lys ttc Phe gca Al a gct Al a 335 cga Ar g gt g Val gt c Val gt g Val ttc Phe 415 t ca Ser ttc Phe aag Lys tct Ser cag G n 240 gt a Val gcc Al a ttt Phe acc Thr cac Hi s 320 ct c Leu t cc Ser t cc Ser tgg Tr p tgg Tr p 400 at g Met gt c Val t ac Ty r t ac Tyr at c SIle 225 gaa G u gcc Al a gag Gu aga Ar g gt g Val 305 gga Gy at g Met aac Asn at t SIle gca Al a 385 gca Al a gga Gy at c SIle at c SIle gtt Val 210 gt g Val ggc Gy gcc Al a aaa Lys aac Asn 290 gct Al a at g Met gca Al a cct Pr o gt a Val 370 ctt Leu ccg Pr o gcg Al a ggc Gy ggt ggt gtc G y G y Val 180 aac gtg ccc Asn Val Pro 195 cct gaa ttc Pro G u Phe ttg tcc atc Leu Ser Ile gaa ggc gct G u G y Al a 245 ttt gcg ctc Phe Al a Leu 260 t cc gga aac Ser G y Asn 275 ttc agc ctc Phe Ser Leu ggt act cct Sy Thr Pro aac gcc at g Asn Ala IVet 325 gca gt a ct g Al a Val Leu 340 gga ct c at g G y Leu IVet 355 ctg ctg tca Leu Leu Ser gtt tcg atg Val Ser bet aac tcg acc Asn Ser Thr 405 ggc tct ggc G y Ser G y 420 gcc gct gcc Al a Al a Al a 435 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 Page 270 seqI i st car r ect ed. t xt atc ggc gcg gta atg cag att cga ctg gca gca ggc gat gag ggc gca IlIe (1y Ala Val [Vbt O31n IIe Ar g Leu Ala Ala (1y Asp (1u (1y Al a 440 445 450 gct t tt ggt caa gca ct t ct a ct t gcc gct gcg gt g ct g gt t at c ggc Al a Phe G31y (31n Al a Leu Leu Leu Al a Al a Al a Val Leu Val Ilie (31y 455 460 465 att gtg gca tca acg atg gca gga aaa aat gca cac cca gcg ccg gta Ile Val Al a Ser Thr rb t Al a G31y Lys Asn Al a Hi s Pr o Al a Pr o Val 470 475 480 485 aag cct t aaaggt cgc at gaat cct t cga Lys Pr o <210> 238 <211> 487 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 238 Val Ser Asp Lys Lys O31n Asp Leu Thr Ser Ser Al a Al a G31y Ser Al a 1459 1 507 1 555 1 584 Al1 Al a Le u Le u Tyr Ser Al a vb t 145 Le u I Ie Tyr Tyr I Ie 225 O31n Pr o O31n Al a Ph e Pr o Le u 115 O31y Thr ValI O31y As n 1 95 Pr o Le u Ly s Le u I Ie Tyr ValI As n cys O31n As n O31y 1 65 ValI Pr o Ph e I Ie Al a I Ie ValI As n 70 Pr a ValI O31y O31y Ar g 150 Ser le le Pr a ValI 230 Tyr Al a Al a O31u Le u Tyr Le u Le u 135 li1e Thr Thr O31y Pr a 215 Al a 10 Al a [Vb t Cys I I e Ser Thr I Ie Tr p Val Thr 90 Al a 03 y 1 Pr o As p Al a Al a Al a Phe O3 y Leu 1 70 As n Tr p 1 1 1 e Ser Thr Ar g Phe Phe Pr a O31y Pr a ValI O31y vb t vb t Le u O31u 1 Al a O31y ValI Pr a Pr a Ph e Le u Ser Le u I Ie Thr 125 Thr Ar g Le u O31n Al a 205 As p Ly s I Ie Al a Tyr As p Thr I Ie O31n Al a O31y 1 ValI vb t Le u Leu Val Phe Al a Phe O31n 235 240 Page 271 seqI i st car r ect ed. t xt (31u (1y (1u 1y Aa (1y Tr p Aa AaTr pVal Tr p 1i1 245 250 e I'b t I Ie Val1 255 A a (31u Ar g ValI 305 G31y vb t As n I Ie A a 385 A a G31y I Ie G31y ValI 465 Hi s Ph e Ser 275 Ph e G31y As n A a G31y 355 Le u ValI As n G31y A a 435 (31u ValI A a Le u As n Le u Pr a vb t 325 Le u vb t Ser vb t Th r 405 G31y A a A a G31y ValI 485 Ph e As p G31y Le u 310 (31u Ser A a vb t Th r 390 Ser ValI I Ie A a I Ie 470 Ly s A a Pr a As n 295 Pr a A a Pr a A a ValI 375 Le u Th r Ph e G31y Ph e 455 ValI Pr a Tr p Le u 280 li1e I Ie G31y Ph e Le u 360 vb t Le u vb t As n A a 440 (31y Ph e 265 ValI cys vb t Ph e ValI 345 G31y I Ie G31y Ar g Th r 425 ValI O31n I Ie Pr a I Ie Le u vb t 330 G31y Ph e Ph e I Ie As p 410 Th r vb t A a Ty r Le u vb t Ty r 315 vb t Ly s Ser As p G31y 395 Le u Ar g O31n Le u vb t 475 O31n I Ie 285 vb t O31n Pr a ValI ValI 365 G31y A a Hi s Le u Ar g 445 Le u Ser 270 Ph e G31y O31n O31n As p 350 A a Le u Ph e Ly s O31y 430 Le u A a Ar g Ly s Ph e A a A a 335 Ar g ValI ValI ValI Ph e 415 Ser A a A a A a Ph e Th r Hi s 320 Le u Ser Ser Tr p Tr p 400 vb t ValI A a A a A a 480 A a Ser Thr A a 03 y Lys Asn <210O> 239 <21 1> 1455 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 OIDS <222> (1432) <223> RXN03064 <400> 239 tggagccttg tcttcctcca gcaatcccac aacggagcag gt tgggat cc cgagaaat gt t gt cat cat c t tggct gt at t agt tt t tac agcct t tgt c at g at g t tg aat gag 115 I'b t I'b t Leu Asn 03 u 1 act act ctg gcagtc gcgttg ccgtcg atc atg gcg gac t tt gac at t 163 Thr Thr Leu Ala Val Ala Leu Pro Ser I Ie I'bt Ala Asp Phe Asp Ilie Page 272 seql i st cor r ect ed. t xt gag G u gct Al a cgt Ar g acg Thr gct Al a gcg Al a at t SIle ggt Gy gtt Val 150 aac Asn at t SIle ggc Gy ggc Gy aag Lys 230 t ac Tyr ggt Gy acc Thr aat Asn gtt Val gt g Val gcg Al a gcg Al a acc Thr gt c Val 120 gt a Val ttg Leu agt Ser gca Al a at t SIle 200 at t SIle gat Asp at t SIle at g Met ttg Leu gcg Al a cca Pr o at t SIle tct Ser ggt Gy gtt Val at g lbt agc Ser ttt Phe cct Pr o 170 gct Al a gaa G u ttg Leu gcg Al a ct g Leu 250 aca Thr gcg Al a cag G n gct Al a tt c Phe cct Pr o 75 acc Thr cct Pr o gcc Al a tt g Leu gt g Val 155 aaa Lys tt c Phe ggt Gy gt g Val ct g Leu 235 gtt Val ct g Leu ggt Gy tgg Tr p act Thr gcc Al a act Thr gct Al a cca Pr o gtt Val tct Ser 140 gca Al a aag Lys ggt Gy gac Asp gt g Val 220 ttg Leu gt g Val ccg Pr o ct a Leu ct c Leu tgg Tr p gt g Val gcg Al a at c l Ie cgc Ar g 110 cct Pr o tgg Tr p ct g Leu cct Pr o ctt Leu 190 agc Ser gt g Val ct g Leu ttg Leu t ac Tyr 270 ct g Leu act Thr at g lbt gt c Val at t l Ie at g lbt 95 cgt Ar g gct Al a cac Hi s at c SIle ttg Leu 175 gt g Val gct Al a tgg Tr p cgt Ar g acg Thr 255 ct g Leu ggt Gy ttg Leu ttc Phe at g lbt ccg Pr o ggc Gy ctt Leu gcg Al a ggt Gy 160 gat Asp t ac Tyr ttg Leu cgc Ar g ccg Pr o 240 ct g Leu cag G n ttt Phe gaa G u ct g Leu ctt Leu ct g Leu gcc Al a gga Gy at t l Ie 145 acc Thr gtt Val gcc Al a gt c Val cag G n 225 ttg Leu ttc Phe gga Gy at g Met cgt Ar g at c SIle gca Al a ct g Leu gt c Val cct Pr o 130 ttc Phe ct g Leu at t SIle ttg Leu gt g Val 210 at t SIle gcg Al a ggt Gy t cc Ser acc Thr acc Thr act Thr cgc Ar g act Thr 100 ggt Gy gtg Val gtc Val ctg Leu ttc Phe 180 tcg Ser gct Al a at g Mat cgt Ar g ctg Leu 260 at g let ttg cca ggt ggt ctt ttg gaa Leu Pro Gy y Leu Leu G u Page 273 seql i st cor r ect ed. t xt 5 290 280 ggt gtg ctg tcg cca ttt gtg ggt cga att Sy Val Leu Ser Pro Phe Val y Arg Ile 295 300 cgc gga ctc gtg atc ggc ggt atg tca ctc Arg y Leu Val I e G y Gy IVet Ser Leu 310 315 gca ctg tcc acc gtc gat gag ttc gcc aac Ala Leu Ser Thr Val Asp G u Phe Ala Asn 330 335 gta cac atc gtg ttc tcc atc ggc ctt gcg Val His I I e Val Phe Ser I I e G y Leu Al a 345 350 atg aca gtc gcg ctc gca tcc gtc ccc gac IVet Thr Val Ala Leu Ala Ser Val Pro Asp 360 365 tcc gcg atc ctc aac acc ctc caa cag ctc Ser Ala lie Leu Asn Thr Leu n G n Leu 375 380 gcg gtc atg att gcg gtt tat tcc acc gtc Ala Val Met Ile Ala Val Tyr Ser Thr Val 390 395 gac ggc gca acc caa caa acc gcc ctc gcc Asp G y Al a Thr G n G n Thr Al a Leu Al a 410 415 ttc ttc gcc tca gcg tgc gtg gca gtg ttt Phe Phe Ala Ser Ala Cys Val Ala Val Phe 425 430 ttt gta aag agg cca gcc cgc taagctaggt Phe Val Lys Arg Pro Al a Arg 440 <210> 240 <211> 444 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 240 NIet NIet Leu Asn G u Thr Thr Leu Ala Val 1 5 10 Ala Asp Phe Asp Ile u Ala Asn Thr Ala 25 Phe Met Leu Thr Met Ala Val Val Leu Pro 40 G u Arg Phe Thr Thr Arg Ser Val Phe lie 55 Leu Ile y Thr Val Thr Ala Ala Leu Ser 70 Leu Ala Ala Arg Val Ala n Ala I e y 90 Leu Leu Met Thr Val Al a Met Thr Val Val Pag t at Tyr gtt Val 320 gt g Val ct g Leu aac Asn gcc Al a agc Ser 400 gac Asp gca Al a gat cgt cat Asp Arg Hi s 305 gtg atc t cc Val Ile Ser tgg ttc atc Trp Phe II e ctg ttc acc Leu Phe Thr 355 atg tac ggc bet Tyr G y 370 ggc gcc gca G y Al a Al a 385 aac aac gcg Asn Asn Al a ggc gcc aac G y Al a Asn ctg atc gt g Leu Ile Val 435 ggt Gy ct g Leu at c SIle 340 cca Pr o cac Hi s ggc Gy ctt Leu tct Ser 420 ggc Gy cca Pr o ttt Phe 325 ggc Gy ct g Leu ggc Gy acc Thr at c SIe 405 gca Al a ttc Phe 1027 1075 1123 1171 1219 1267 1315 1363 1411 1455 cgcat gat ca gca Al a Leu Sn Trp Al a Thr Phe Al a Pro Thr Thr Al a Pro Pro e 274 Pr o Leu Sy Thr Phe Val G u Ser Leu Tr p Val Al a SIle Ar g I e Thr bet Val SIe Met Ar g seql i st cor r ect ed. t xt Al a Val MVet 115 G y Pro Ser 130 I e Phe Tr p 145 Thr Leu Ar g Val Ile Ser Ala Leu Ser 195 Val Val Leu 210 G n Ile Al a 225 Leu Al a Il e Phe G y Al a G y Ser Leu 275 G y G y Leu 290 Asp Arg Hi s 305 Val Ile Ser Trp Phe Ile Leu Phe Thr 355 Met Tyr G y 370 G y Al a Al a 385 Asn Asn Al a G y Al a Asn Leu Ile Val 435 <210> 241 <211> 1093 Leu Al a Met Thr 165 Leu SIle Val Sy G u 245 Leu Val G u Pr o Phe 325 Sy Leu Sy Thr l Ie 405 Al a Phe SIle Gy Val 150 Asn SIle Sy Sy Lys 230 Tyr Sy Thr Sy Ar g 310 Al a Val bet Ser Al a 390 Asp Phe Phe Al a Phe 135 Pr o Val Ser I e I e 215 G n Thr Val Al a Val 295 Sy Leu Hi s Thr Al a 375 Val Sy Phe Val Val 120 Val Leu Ser Al a SIle 200 I le Asp SIle bet Leu 280 Leu Leu Ser SIle Val 360 l le Met Al a Al a Lys 440 Val Met Ala Val G y Pro 125 Leu Ser Leu Ser Ser Trp 140 Val Phe Val Al a Ser Leu 155 G u Pro Lys Lys Thr Pro 170 Val Ala Phe y y Leu 185 190 Leu G u G y Asp Arg Ser 205 Ala Leu Val Val Phe Val 220 Lys Ala Leu Leu Asp Leu 235 Pro Leu Val Val Leu Leu 250 Asn Thr Leu Pro Leu Tyr 265 270 Val Ala y Leu Val Leu 285 Ser Pro Phe Val y Arg 300 Val Ilie y y bet Ser 315 Thr Val Asp G u Phe Ala 330 Val Phe Ser Ile y Leu 345 350 Ala Leu Ala Ser Val Pr o 365 Leu Asn Thr Leu G n G n 380 Ile Ala Val Tyr Ser Thr 395 Thr G n G n Thr Ala Leu 410 Ser Ala Cys Val Ala Val 425 430 Arg Pro Ala Arg Al a Hi s SIe Leu 175 Val Al a Tr p Ar g Thr 255 Leu Leu SIe Leu Asn 335 Al a Asp Leu Val Al a 415 Phe Page 275 seql i st cor r ect ed. t xt <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1093) <223> FRXA00565 <400> 241 tggagccttg tcttcctcca gcaatcccac aacggagcag gttgggatcc cgagaaatgt tgtcatcatc ttggctgtat tagtttttac agcctttgtc atg atg ttg aat gag IVet IVet Leu Asn G u act Thr gag Gu gct Al a cgt Ar g acg Thr gct Al a gcg Al a at t SIle ggt Gy gtt Val 150 aac Asn at t SIle ggc Gy ct g Leu aat Asn gtt Val gt g Val gcg Al a gcg Al a acc Thr gt c Val 120 gt a Val ttg Leu agt Ser gca Al a at t SIle 200 gt c Val gcg Al a cca Pr o at t SIle tct Ser ggt Sy gtt Val at g let agc Ser ttt Phe cct Pr o 170 gct Al a gaa Gu gcg Al a cag G n gct Al a tt c Phe cct Pr o 75 acc Thr cct Pr o gcc Al a tt g Leu gt g Val 155 aaa Lys tt c Phe ggt Gy ttg Leu tgg Tr p act Thr gcc Al a act Thr gct Al a cca Pr o gtt Val tct Ser 140 gca Al a aag Lys ggt Gy gac Asp ccg Pr o ttg Leu ggt Gy 45 acg Thr ttt Phe gt g Val gag G u ggt G y 125 tcg Ser agc Ser act Thr ggc Gy aga Ar g 205 tcg Ser ct c Leu tgg Tr p gt g Val gcg Al a at c SIle cgc Ar g 110 cct Pr o tgg Tr p ct g Leu cct Pr o ctt Leu 190 agc Ser at c I e 15 act Thr at g Met gt c Val at t l Ie at g Met 95 cgt Ar g gct Al a cac Hi s at c SIle ttg Leu 175 gt g Val gct Al a at g Met ggt Gy ttg Leu ttc Phe at g Met ccg Pr o ggc G y ctt Leu gcg Al a ggt Gy 160 gat Asp t ac Tyr ttg Leu 1 gcg Al a ttt Phe gaa Gu ct g Leu ctt Leu ct g Leu gcc Al a gga G y at t l Ie 145 acc Thr gtt Val gcc Al a gt c Val gac Asp at g Met cgt Ar g at c SIle gca Al a ct g Leu gt c Val cct Pr o 130 ttc Phe ct g Leu at t SIle ttg Leu gt g Val 210 gac Asp acc Thr acc Thr act Thr cgc Ar g act Thr 100 ggt G y gtg Val gtc Val ctg Leu ttc Phe 180 tcg Ser gct Al a ggc atc att gcg ttg gtg gtg ttt gtg tgg cgc cag att gcc atg ggt Page 276 G31y aag Ly s 230 t ac Tyr ggt G31y ac c Thr ggt G31y cgc Ar g 310 gca Al a I Ie g at As p at t I Ie at g vb t t tg Le u 280 ct g Le u ct c Le u t cc Ser Le u gcg Al a ct g Le u 250 ac a Thr gcg Al a cca Pr o at c I Ie gt c ValI 330 ValI 220 t tg Le u gt g ValI ccg Pr o ct a Le u gt g ValI 300 ggt O31y seqI i st car r ect ed. t xt Phe Val Tr p Arg O31n I Ie 225 gat ct g cgt ccg t tg gcg Asp Leu Arg Pro Leu Ala 240 ct t t tg acg ct g t tc ggt Leu Leu Thr Leu Phe 03 y 255 ct c t ac ct g cag gga t cc Leu Tyr Leu O31n G31y Ser 270 gt g ct g t tg cca ggt ggt Val Leu Leu Pr o (31y (31y 285 290 ggt cga at t t at gat cgt G31y Ar g Ilie Tyr Asp Ar g 305 at g t ca ct c gt t gt g at c I'b t Ser Leu Val Val Ilie 320 Al a at t I Ie gcg Al a tt g Le u 275 ct t Le u cat Hi s t cc Ser vb t cgt Ar g ct g Le u 260 at g vb t tt g Le u ggt G31y ct g Le u G31y gag O31u 245 ct c Le u gt c ValI g aa O31u cca Pr o tt t Ph e 325 835 883 931 979 1 027 1 075 1 093 <210O> 242 <21 1> 331 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 242 I'bt I'bt Leu Asn (3 u Thr Thr Leu P 1 5 Al aAsp Phe Asp 11 e 03 u Al a As n T Phe I'bt Leu Thr I'b t Al a ValI Val L 40 O31u Ar g Phe Thr Thr Ar g Ser Val F 55 Leu I Ie G31y Thr Val Thr Al a Al a L 70 Leu Al a Al a Ar g Val Al a O31n Al a 1 Leu Leu I'b t Thr Val Al a I'b t Thr\ 100 1 Al a Val I'b t G31y Leu I Ie Al a Val\ 115 120 G31y Pr o Ser Val Al a G31y Phe Val L 130 135 li1e Phe Tr p Val I'b t Val Pr o Leu\ 145 150 Ja Val Ala Leu Pro Ser Ilie I'bt hr .eu ~he .eu Ile .eu 10 Ala O31n Tr p Pr o Al a Thr I I e Phe Al a Ser Pr o Thr O3 y Thr Al a 90 Val Pr o Pr o I'b t Al a Val Ser Leu Ser 140 Phe Val Al a 1 Page 277 Le u O31y Thr Ph e ValI O31u O31y 125 Ser Ser Thr vb t ValI I Ie vb t Ar g Al a Hi s I Ie NO seql i st cor r ect ed. t xt Thr Leu Arg Leu Thr Asn Val Ser G u Pro Lys Lys Thr Pro Leu Asp 165 170 175 Val Ile Ser Phe Leu I e Ser Ala Val Ala Phe Gy y Leu Val Tyr 180 185 190 d) 4 Ala Leu Ser Ser lie Gy lie lie Leu u Gy Asp Arg Ser Ala Leu 195 200 205 Val Val Leu Ala Val G y Ile Ile Ala Leu Val Val Phe Val Trp Arg 210 215 220 Sn Ile Ala IVet y Lys G n Asp Lys Ala Leu Leu Asp Leu Arg Pro S225 230 235 240 00 0 Leu Ala lie Arg G u Tyr Thr lie Pro Leu Val Val Leu Leu Thr Leu 245 250 255 \O Phe y Al a Leu Leu G y Val IVet Asn Thr Leu Pro Leu Tyr Leu G n 260 265 270 C 1 G y Ser Leu Vet Val Thr Ala Leu Val Al a y Leu Val Leu Leu Pro 275 280 285 Gy y Leu Leu G u G y Val Leu Ser Pro Phe Val y Arg Ile Tyr 290 295 300 Asp Arg His G y Pro Arg G y Leu Val IIe G y G y Iet Ser Leu Val 305 310 315 320 Val lie Ser Leu Phe Ala Leu Ser Thr Val Asp 325 330 <210> 243 <211> 380 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA02878 <400> 243 t gc ctg tcc acc gtc gat gag tt c gcc acg tgt tgg t ca t ca tt c gcg 48 Cys Leu Ser Thr Val Asp u Phe Ala Thr Cys Trp Ser Ser Phe Ala 1 5 10 gac aca tcg tgg ttc tca tcg gcc ctt gcg ctg ctg ttc acc cca ctg 96 Asp Thr Ser Trp Phe Ser Ser Ala Leu Ala Leu Leu Phe Thr Pro Leu 25 atg aca gtc gcg ctc gca tcc gtc ccc gac aac atg tac ggc cac ggc 144 IVet Thr Val Ala Leu Ala Ser Val Pro Asp Asn IVet Tyr G y Hi s G y 40 tcc gcg atc ctc aac acc ctc caa cag ctc gcc ggc gcc gca ggc acc 192 Ser Ala lie Leu Asn Thr Leu n n Leu Ala Gy Ala Ala y Thr 55 gcg gtc atg att gcg gtt tat tcc acc gtc agc aac aac gcg ctt atc 240 Ala Val IVet Ile Ala Val Tyr Ser Thr Val Ser Asn Asn Ala Leu Ile 70 75 gac ggc gca acc caa caa acc gcc ctc gcc gac ggc gcc aac tct gca 288 Asp G y Ala Thr G n G n Thr Ala Leu Ala Asp G y Ala Asn Ser Ala 90 Page 278 seql i st cor r ect ed. t xt ttc ttc gcc tca gcg tgc gtg gca gtg ttt gca ctg atc gtg ggc ttc Phe Phe Ala Ser Ala Cys Val Ala Val Phe Ala Leu I e Val y Phe 100 105 110 ttt gta aag agg cca gcc cgc taagctaggt cgcatgatca gca Phe Val Lys Arg Pro Al a Arg 115 <210> 244 <211> 119 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 244 Cys Leu Ser Thr Val Asp G u Phe Ala 1 5 Asp Thr Ser Trp Phe Ser Ser Ala Leu 25 Met Thr Val Ala Leu Ala Ser Val Pro 40 Ser Ala lie Leu Asn Thr Leu G n G n Ala Val Met I e Ala Val Tyr Ser Thr 70 Asp G y Al a Thr G n G n Thr Al a Leu Phe Phe Ala Ser Ala Cys Val Ala Val 100 105 Phe Val Lys Arg Pro Al a Arg 115 Thr 10 Al a Asp Leu Val Al a 90 Phe Cys Leu Asn Al a Ser Asp Al a Ser Phe Tyr Al a Asn Al a l Ie Ser Thr Gy Al a Al a Asn Val 110 Phe Pr o Hi s Gy Leu Ser Gy <210> 245 <211> 1533 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1510) <223> RXA00648 <400> 245 gtttgccagc tattgcatct tgcacaaaat gtgtaccata cacataatgt catcgagtcc tcccgaatca gccacaccac agatcaaata cggcctgctg gtg gtc act ctc gcc Val Val Thr Leu Ala 1 tca gct ggt atc act gtt tcc cta gcg cag acc ctg gtt att ccg atc Ser Ala Gy lle Thr Val Ser Leu Ala n Thr Leu Val Ile Pro Ile 15 att ggt cgg ttg ccc gag atc ttc aac acc acg gct gct aat gcc tct lie y Arg Leu Pro G u lie Phe Asn Thr Thr Ala Ala Asn Ala Ser 30 tgg atc att act gtg acg ctg ttg gtg ggc gca gtg gcg act cct gtg Trp lie Ile Thr Val Thr Leu Leu Val Gy Ala Val Ala Thr Pro Val Page 279 seql i st cor r ect ed. t xt at g Met t ca Ser gat Asp ggc Gy gag G u ggc Gy t cc Ser 150 ggc Gy agt Ser at c SIle agt Ser ggt Gy 230 cgc Ar g ct c Leu at g Met cag G n tcg Ser agg Ar g gt c Val at t SIle at t SIle gca Al a 120 gca Al a cgg Ar g gt c Val ggc Gy ttg Leu 200 ct g Leu ggc Gy act Thr ggt Gy ct g Leu 280 ggc Gy gca Al a gca Al a ttc Phe at g Met ctt Leu tct Ser ggt Gy ct g Leu tgg Tr p 170 gat Asp ct c Leu at t SIle ttc Phe cgg Ar g 250 acc Thr gt a Val tgg Tr p gca Al a gat Asp at t SIle 75 at c SI e ggc Gy gcc Al a ct a Leu tt c Phe 155 aag Lys t at Tyr gcg Al a ggg Gy gaa G u 235 gcg Al a at g bet at t SIle ct g Leu gcc Al a at g Met ctt Leu at c SIle at t SIle at t l Ie ccg Pr o 140 tgg Tr p gcg Al a ttc Phe gt g Val tta Leu 220 acc Thr acc Thr t at Tyr ct g Leu at c SIle 300 at t l Ie t ac Tyr gga Gy ggc Gy tct Ser gct Al a 125 ct g Leu ttc Phe at t l Ie ggt Gy t cc Ser 205 ttc Phe cgc Ar g gt g Val gga Gy ggc Gy 285 ccg Pr o agc Ser ggc aag aaa G y Lys Lys t ca gt g at c Ser Val I le cgt ggt ttt Arg G y Phe 95 ct c at g cat Leu Met Hi s 110 ttg atg agt Leu IVet Ser gct gct gct Al a Al a Al a acc gct ct g Thr Ala Leu 160 cct gct aga Pro Al a Ar g 175 gct ct c ggc Al a Leu G y 190 aag gga tca Lys G y Ser gtg gca gcg Val Al a Al a cag aaa tcc G n Lys Ser 240 ttg atg aca Leu IVet Thr 255 atg aat ct g IVet Asn Leu 270 tac ggt ct a Tyr G y Leu atg ggt cta IVet G y Leu gct gct cat Al a Al a Hi s at g Met gct Al a ggg Gy ttg Leu t cc Ser 130 gcc Al a gcg Al a agg Ar g gca Al a tgg Tr p 210 gt g Val ttg Leu at t SIle ct g Leu cag G n 290 at g Met ct c Leu tcg Ser ggc Gy 100 ccc Pr o ggc Gy ttt Phe aca Thr gt g Val 180 gga Gy tgg Tr p ttg Leu gat Asp t cc Ser 260 cag G n at g Met ttg Leu 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 ggt cct cgt gtg acg Gy Pro Arg Val Thr Page 280 310 ct g Le u ac a Thr aac As n gt g ValI at c I Ie 390 aac As n ggt G31y gga G31y gcg Al a seqI i st car r ect ed. t xt 320 aca att gcg Thr I Ie Al a gt g t tg tt c Val Leu Phe 345 gca ct t at t Al a Leu Il e 360 gt c ggt at c Val G31y Ilie 375 at g ggt gcc [Vb t 31y Al a t ct ct t at g Ser Leu I'b t gca gt g tt g Al a Val Leu 425 ttc atg acc Phe I'b t Thr 440 gt c at c t cc Val Ilie Ser 455 ggt G31y 330 act Th r tt g Le u ggc G31y gt a ValI cgt Ar g 410 gcc Al a act Th r t at gt t ValI at c I Ie acc Th r at t I Ie cca Pr o 395 t ca Ser gga G31y ct g Le u t t c gt g ValI G31y ac c Th r ggc G31y 380 gcc Al a ct g Le u at g vb t at c I Ie at c at c gca I e Al a aac cgc Asn Arg 350 ct g gt g Leu Val 365 ct g gca Leu Al a acg gag Thr G3 u ggc acc G3 y Thr at g agt I'b t Ser 430 at c gga I Ie G31y 445 ccc acc Pr o Thr Tyr Phe I Ie 460 gt c ggt 1 Val 31y~ 335 aca ccg Thr Pr o ct g t tc 1 Leu Phe t tt ggt 1 Phe G3 y aaa gcc Lys Al a 400 acc ggc 1 Thr G3 y 415 ggc gga (31y 1y~ t gc t gc cys cys aca acc Thr Thr Thr Val 10 Pr o au1u Val Thr I Al a Asp I Phe Il e 1 I'b t Ilie 90 Leu a3 y Page 281 [at Ty r gga [ca Se r [cc Se r 385 gca Al a [ca Se r gt a ValI gcc Al a act Th r 465 gca Al a gga a31y gt g ValI 370 at g vb t gcg Al a t ca Ser ccc Pr o gcg Al a 450 gt g ValI act Th r 340 gca Al a agt Ser gcc Al a ggt a31y gtc ValI 420 tt a Le u gt g ValI g aa a31u gcc Al a g ac As p ct c Le u tt g Le u tt c Ph e 405 at c I Ie ggg a31y gct Al a gca Al a 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 533 aaa t aat cccggc agcgact cga cca Lys 470 <210> 246 <211> 470 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 246 Val Val Thr Leu Ala Ser Ala a1y Ilie 1 5 Leu Val I Ie Pro I Ie I Ie a31y Ar g Leu 25 Ala Ala Asn Ala Ser Trp Ilie lieThr 40 Val Al a Thr Pr o Val I'b t a31y Ar g Leu Lys I'b t I'b t Leu Ilie Ser Leu Val Pr o 70 Cys Al a Val Ser Val Asp Leu I Ie Pro a31n a31y Leu a31y Ser a31y Leu I Ie Pro Le u Ph e Le u Tyr a31y a31y Ser Al a As n ValI a31y Ser Ar g Le u a31n Th r a31y Lys ValI a31y vb t seqI i st car r ect ed. t xt As p Ser I Ie 145 ValI Pr o Le u (31u Le u 225 Pr o As n I Ie G31y G31y 305 G31y Tyr G31y Ser Ser 385 A a Ser ValI Le u 115 O31n Le u I Ie vb t 1 95 Gy le le A a Pr o 275 Ser vb t Ar g Le u As p 355 cys Pr o As n A a Thr 435 Ar g I Ie A a ValI 1 65 Ar g Le u Ar g ValI Le u 245 li1e ValI Le u I Ie Thr 325 A a As p Le u Le u Ph e 405 li1e (31y Ly s (31y 135 Tr p A a G31y A a A a 215 Tr p Thr I Ie O31n vb t 295 As n Thr ValI A a ValI 375 vb t Ser A a Ph e 1 A a 03 y Se r 120 A a Leu 03 y Ar g Val Leu Val I Ie Tr p 1 70 O3 y Phe As p 1 85 Leu Leu Leu 200 Leu Thr Ilie G31y Tr p Phe Thr Ilie Ar g 250 O3 y Phe Thr 265 Leu Pr o Val 280 G31y Ilie Tr p A a 03 y A a Ile A a 03 y 330 Leu Phe Thr 345 Leu Il e Leu 360 31y Ilie G31y O3 y A a Val Leu I'b t Ar g 410 Val Leu A a 425 I'b t Thr Thr 440 A a I I e A a 125 Leu Pro Leu 140 Phe Trp Phe 1 Lys A a IlIe Tyr Phe (31y A a Val Ser 205 O3 y Leu Phe 220 O31u Thr Ar g 235 A a Thr Val I'b t Tyr (3 y Il e Leu 03 y 285 Leu Ilie Pr o 300 A a Il e Ser 315 Val Val Ilie Il e 03 y Asn Thr Thr Leu 365 Il e 03 y Leu 380 Pr o Ala Thr 395 Ser Leu G31y 31y Ib t [vb t Leu Il e Il e 445 Le u A a Thr Pr a A a 1 Ly s ValI O31n Le u vb t 270 Tyr vb t A a A a Ar g 350 ValI A a O31u Thr Ser 430 G31y vb t A a A a A a 1 Le u G31y A a Ly s vb t 255 As n G31y G31y A a ValI 335 Thr Le u Ph e Ly s Th r 415 G31y Cys Ala Ala Leu Val Ala Ala Val Ilie Ser Tyr Phe Ilie Pro Thr Thr Thr 450 455 460 Page 282 seal i st car r ect ed. t xt Thr Val Val 3u Ala Lys 465 470 <210> 247 <211> 1770 <212> DNA <213> Cor ynebact er i ur gl ut ani cum <220> <221> CDS <222> (101)..(1747) <223> RXN01320 <400> 247 gtgaatggca cgacatgcca caaggcacgc aagctgattt caattaaaaa tacttttctt cttagaggtg gattttcaga ccaagcctgc tgtcgcaaag atg aca tca cag gtc 11! l'bt Thr Ser 0 n Val aag Lys cct Pr a gct Al a ct C Leu t ct Ser acc Thr at t SI e ttt Phe cag O31n ggt 3y 150 acc Thr gga (31y gac Asp gcc Al a gag O31u ttg Leu ct g Leu gt g Val ggc G31y at c SI e 120 at g lvb t ggc G31y cgt Ar g t cc Ser g aa O31u acc Thr aaa Lys at g lvb t acg Thr acc Thr ttg Leu ct g Leu acc Thr at g rvb t cgt Ar g 170 at c SI e cgt Ar g t ca Ser acc Thr ct t Leu at t SI e 75 gcc Al a ggt G31y tt C Phe tt g Leu at t SI e 155 gca Al a ct t Leu ccg Pr a gca Al a aaa Lys ct t Leu gt t Val ttc Phe gac Asp gt g Val at t SI e 140 ct t Leu aag Lys (31y gt a Val cca Pr a ggt G31y 45 agc Ser ggt G31y ct C Leu cag O31n gt g Val 125 gt g Val t ct Ser t ac Tyr cca Pr a aca Thr t at Tyr cgc Ar g t cc Ser gag O31u ttg Leu ttt Phe 110 ggt G31y gct Al a cag O31n at g ttg Leu 190 aca Thr 15 ggt G31y gt t Val ttg Leu ct t Leu ggc G31y 95 ggt G31y t cc Ser cgt Ar g gca Al a ggc G31y 175 ct t Leu at t SI e gca Al a gga G31y ggt G31y ggc G31y 80 cag O31n cgc Ar g at c SI e gca Al a at t SI e 160 at c SI e ggt (31y 1 t ca Ser gca Al a ttt Phe cag O31n 65 ggc O31y acc Thr aaa Lys at c SI e ct g Leu 145 acc Thr at g Gyb t aaa Lys gca Al a at c SI e acc Thr gt t Val at t SI e t ac Tyr ggt O31y 130 cag O31n gct Al a ggt O31y t gg Tr p agt Ser act Thr 35 at c SI e at t SI e aac Asn t ca Ser ct C Leu 115 gct Al a ggt O31y gat Asp t cc Ser ttc Phe 195 ggt O31y gaa O31u gca Al a ttc Phe cac Hi s ttg Leu 100 ttc Phe ttg Leu at c SI e gt c Val gt t Val 180 act Thr gca Al a gaa O31u gcc Al a ggt O31y at g cct Pr a at g lvb t gct Al a gcc Al a acc Thr 165 ttc Phe gac Asp Page 283 seql i st cor r ect ed. t xt ggt Gy at c SIle cgt Ar g 230 gcc Al a gag G u gct Al a gt c Val gcc Al a 310 cct Pr o ct g Leu gt g Val at c SIle aca Thr 390 gga G y aac Asn ttc Phe ct g Leu ggc Gy 200 ct g Leu aag Lys acc Thr gca Al a at a l Ie 280 at g Met at c l Ie t ac Tyr ct g Leu aac Asn 360 at g Met tcg Ser G y ttc Phe cgc Ar g 440 at c SIle cgt Ar g gct Al a t cc Ser ttt Phe 250 cca Pr o ttc Phe ctt Leu gt a Val cag G n 330 cca Pr o gt g Val at c SI e agc Ser ggc G y 410 at c SIle at c SIle aac Asn ggt Gy gct Al a 220 gac Asp ct c Leu at c SIle ttc Phe tcg Ser 300 ct g Leu gtt Val at g Met aag Lys gt c Val 380 gct Al a gca Al a at g Met gga G y t cc Ser 460 ctg tgg ttg aac Leu 205 gt g Val tgg Tr p gca Al a at c SIle gt c Val 285 aac Asn ttt Phe cat Hi s at c SI e act Thr 365 ctt Leu ct c Leu at g Met gtt Val gca Al a 445 gac Asp Trp Leu Asn ct g Leu ttg Leu gt g Val ggt G y 270 gaa Gu cgc Ar g at g Met ggt Gy ggc Gy 350 ggc Gy gcc Al a at t SIle cag G n ggc G y 430 gt a Val cga Ar g ct g Leu gga G y acc Thr 255 ttg Leu aag Lys aac Asn at g Met ct g Leu 335 ct g Leu aag Lys ct a Leu gga G y at t SIle 415 acc Thr ggt G y aaa Lys agc Ser 240 tgg Tr p ttc Phe cgt Ar g ttc Phe ggc Gy 320 aac Asn at t SIle t ac Tyr gt a Val ct g Leu 400 ttg Leu gcg Al a t cc Ser gtt Val ctt Leu 225 at c SIle ggt G y at c SIle gct Al a gt g Val 305 acc Thr cca Pr o ggt Gy aag Lys ct g Leu 385 t ac Tyr gtt Val acc Thr gca Al a cca at c ggc Pro lie y 210 cca gct cgt Pro Al a Ar g ttc atg gct Phe bet Al a ggc aat gaa G y Asn G u 260 acg aca ttg Thr Thr Leu 275 gtt gac cca Val Asp Pro 290 ctc acc gcc Leu Thr Al a at c gcg t ac I le Al a Tyr acg caa gct Thr G n Al a 340 aca t cc act Thr Ser Thr 355 tgg tac cca Trp Tyr Pro 370 ct a t cg acg Leu Ser Thr ttc ttc gtc Phe Phe Val ct c at c gt g Leu Ile Val 420 ggt t cc aac G y Ser Asn 435 ct g at c ggt Leu Ile G y 450 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 ttc acc gaa aac gtc ccc gca Phe Thr G u Asn Val Pro Ala 465 Page 284 seqI i st car r ect ed. t xt gca gt g gct t cc at g ggt gaa gaa ggc gca caa t ac gcc Ala Val Ala Se r 'b t O3y(3u (1u (1y Aa 1n Tyr Ala 470 475 480 t cc gat t tc t cc ggt gca t cc aac ct c act cca cac ct t Ser Asp Phe Ser G31y Ala Ser Asn Leu Thr Pr o Hi s Leu 490 495 ct t cca caa gca ct c cgt gaa gca at t caa ct t t ct t ac Leu Pr o O31n Ala Leu Ar g O31u Ala Ilie O31n Leu Ser Tyr 505 510 ct g aca cca at c t tc t tg gcg ct c acc ccg at c gca gt a Leu Thr Pr o Ilie Phe Leu Ala Leu Thr Pr o Ilie Ala Val 520 525 530 at c ct c ct c t tt t tc at c cgt gaa gat cac ct c aag gaa Ilie Leu Leu Phe Phe Ilie Ar g O31u Asp Hi s Leu Lys O31u 535 540 545 t aat gacaca cgaaacttcc gt c <210> 248 <211> 549 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn t ca Ser gt t ValI aac As n 515 gt c ValI ac g Thr gca A a g aa O31u 500 g ac As p gcc A a c ac Hi s at g vb t 485 t ca Ser gcc A a gcg A a g aa aOu 1 555 1 603 1651 1 699 1 747 1 770 <400> 248 I'bt Thr Ser Ser Lys Ser A a A a Thr Phe I I e I I e O31n Thr I Ie 31y Val As n Thr I Ie Ser Lys Ty r Leu 115 1I e 03 y A a 130 Leu 03 n 03 y 145 Thr A a As p I'b t G31y Ser (3y Tr p Phe 1 95 O31n Val Lys Pro Asp Asp A a O31u A a G31y vb t Pr a vb t A a A a Thr 1 65 Ph e As p Pr a A a Le u Ser 70 Thr I Ie Ph e O31n O31y 150 Thr O31y (31y Ser ValI vb t 55 A a Tr p Ph e A a As n 135 O31y A a Le u Pr a A a O31u 40 Le u Le u ValI O31y I Ie 120 vb t O31y Ar g Ser (31y 200 Hi s O31u A a Pr a I Ie Lys5 1 A a Thr Le u O31u Ser 1 Tr p Ar g Ser Thr Le u I Ie A a O31y Ph e Le u I Ie 1 A a Le u Pr a A a Lys5 Le u ValI Ph e As p ValI I Ie 140 Le u Lys5 O31y ValI Pr a O31y Ser O31y Le u O31n ValI 125 ValI Ser Tyr Pr a Thr O31y ValI Le u Le u O31y O31y Ser Ar g A a O31y 1 Le u Ar g Tr p (3y Le u Tr p Le u As n 205 Page 285 seqI i st car r ect ed. t xt Val Pro lie y Ilie lieAla Leu Val 210 215 Ala Ilie Ala Val Leu Leu Lys Le u 225 li1e Gy le Al a ValI 305 Thr Pr o G31y Ly s Le u 385 Tyr ValI Thr Al a (31u 465 Tyr Hi s Ser Al a Ly s 545 Al a vb t As n Thr 275 As p Thr Al a G n Ser 355 Tyr Ser Ph e I Ie Ser 435 li1e ValI Ser ValI As n 515 ValI Thr Ou le 245 Tyr ValI Le u ValI vb t 325 G y ValI Ph e Le u Ph e 405 O31n As n G31y Al a vb t 485 Ser Al a Al a (31u Ar g 230 Al a (31u Al a ValI Al a 310 Pr o Le u ValI I Ie Thr 390 G31y As n Ph e Le u Al a 470 Ser Le u Le u I Ie G31y Thr Tr p Al a Pr o 295 G31y Thr vb t G31y G31y 375 Pr o Ph e Ser Ph e Ph e 455 ValI As p Pr a Thr Le u 535 ValI Al a Ser 265 ValI G31y G31y Le u I Ie 345 li1e Le u Al a Le u Pr a 425 O31n Ser Ser Ser Al a 505 li1e Ph e ValI 235 ValI vb t ValI Ph e G31y 315 vb t b t Ser vb t Le u 395 cys Thr G31y Le u G31y 475 Al a Ar g Le u I Ie 220 As p Le u I Ie Ph e Ser 300 Le u ValI vb t Ly s ValI 380 Al a Al a vb t G31y Ser 460 O31u Ser O31u Al a Ar g 540 Tr p Al a I Ie ValI 285 As n Ph e Hi s I Ie Thr 365 Le u Le u vb t ValI Al a 445 As p O31u As n Al a Le u 525 O31u G31y Thr 255 Le u Ly s As n vb t Le u 335 Le u Ly s Le u G31y 415 Thr G31y Ph e Al a Th r 495 O31n Pr a Hi s Page 286 seqi i st car r ect ed. t xt <210> 249 <211> 841 <212> DNA <213> Cor ynebact er i ur gl ut ani cum <220> <221> CDS <222> (101)..(841) <223> FRXA01314 <400> 249 gtgaatggca cgacatgcca caaggcacgc aagctgattt caattaaaaa tacttttctt cttagaggtg gattttcaga ccaagcctgc tgtcgcaaag aag Lys cct Pr o gct Al a ct C Leu t ct Ser acc Thr at t SI e ttt Phe cag O31n ggt 3y 150 acc Thr gga G31y ggt 3y gac Asp gcc Al a gag O31u ttg Leu ct g Leu gt g Val ggc G31y at c SI e 120 at g lvb t 99t ggc G31y cgt Ar g t cc Ser ggc (31y 200 g aa O31u acc Thr aaa Lys at g lvb t acg Thr acc Thr ttg Leu ct g Leu acc Thr at g rvb t cgt Ar g 170 at c SI e cgt Ar g cgt Ar g t ca Ser acc Thr ct t Leu at t SI e 75 gcc Al a ggt G31y tt C Phe tt g Leu at t SI e 155 gca Al a ct t Leu t gg Tr p ccg Pr o gca Al a aaa Lys ct t Leu gt t Val ttc Phe gac Asp gt g Val at t SI e 140 ct t Leu aag Lys ggc 3y ggt (31y gt a Val cca Pr o ggt G31y 45 agc Ser ggt O31y ct C Leu cag O31n gt g Val 125 gt g Val t ct Ser t ac Tyr cca Pr o ct g Leu 205 aca Thr t at Tyr cgc Ar g t cc Ser gag O31u ttg Leu ttt Phe 110 ggt G31y gct Al a cag O31n at g ttg Leu 190 t gg Tr p aca Thr 15 ggt G31y gt t Val ttg Leu ct t Leu ggc G31y 95 ggt G31y t cc Ser cgt Ar g gca Al a ggc G31y 175 ct t Leu ttg Leu at t SI e gca Al a gga G31y ggt G31y ggc G31y 80 cag O31n cgc Ar g at c SI e gca Al a at t SI e 160 at c SI e ggt O31y aac Asn at g rvb t 1 t ca Ser gca Al a ttt Phe cag O31n ggc O31y acc Thr aaa Lys at c SI e ct g Leu 145 acc Thr at g lvb t ggc O31y gt t Val aca tca cag Thr Ser 3 n aaa Lys gca Al a at c SI e acc Thr gt t Val at t SI e t ac Tyr ggt O31y 130 cag O31n gct Al a ggt O31y t gg Tr p cca Pr a 210 ggt O31y g aa O31u gca Al a ttc Phe cac Hi s ttg Leu 100 ttc Phe ttg Leu at c SI e gt c Val gt t Val 180 act Thr ggc (31y gt c Val gca Al a gaa O31u gcc Al a ggt O31y at g cct Pr a at g lvb t gct Al a gcc Al a acc Thr 165 ttc Phe gac Asp at c SI e Page 287 seqI i st car r ect ed. t xt at c gca ct g gt t gct at c gct gt g ct g ct g aaa ct t cca gct cgt gaa I Ie Al a Leu Val Al a I Ie Al a Val Leu Leu Lys Leu Pro Ala Arg O31u 215 220 225 cgt ggc aaggt c tcc gtt gac tggt t ggga agc at ct t cat ggct at c Ar g G31y Lys Val Ser Val Asp Tr p Leu G31y Ser Ilie Phe I'bt Al a Ilie 230 235 240 245 gcc acc Al a Thr <210> 250 <211> 247 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 250 I'bt Thr Ser Ser Lys Ser Al a Al a Thr Phe I I e I I e O31n Thr I Ie 31y Val As n Thr I Ie Ser Lys Ty r Leu 115 1 e 03 y Al a 130 Leu 03 n 03 y 145 Thr Al a As p I'b t G31y Ser G31y Tr p Phe 1 95 Val Pr o I Ie 210 Leu Pr o Al a 225 O3 n Val1 O3 y Al a (31u (1u Al a Al a Phe 03 y Hi s I'b t Leu Pro 1 00 Phe I'b t Leu Al a I e Al a Val Thr 1 65 Val Phe 1 80 Thr As p G31y I Ie Ar g G31u Lys Pro Asp Asp Ser ValI vb t 55 Al a Tr p Ph e Al a As n 135 G31y Al a Le u Pr a Al a 215 G31y Al a G31u 40 Le u Le u ValI G31y I Ie 120 vb t G31y Ar g Ser G31y 200 Le u Ly s Hi s G31u Al a Pr a I Ie Ly s 1 Al a Thr Le u G31u Ser 1 Tr p ValI ValI G31u 10 Thr Ly s vb t Thr Thr 90 Le u Le u Thr vb t Ar g 1 70 li1e Ar g Al a Ser Ar g Ser Thr Le u I Ie Al a G31y Ph e Le u I Ie 1 Al a Le u Tr p I Ie ValI 235 Pr a Al a Ly s Le u ValI Ph e As p ValI I Ie 140 Le u Ly s G31y G31y Al a 220 As p ValI Pr a G31y Ser G31y Le u O31n ValI 125 ValI Ser Tyr Pr a Le u 205 ValI Tr p Thr G31y ValI Le u Le u G31y G31y Ser Ar g Al a G31y 1 Le u Le u Le u G31y Il e Phe I'bt Al a Il e Al aThr 245 Page 288 seqi i st car r ect ed. t xt <210O> 251 <21 1> 803 <212> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (780) <223> FRXA01320 <400> 251 gt t ValI ct c Le u at c I Ie ac g Th r ac a Th r t gg Tr p ct a Le u tt c Ph e ct c Le u ggt G31y 145 ct g Le u aac As n gcc A a cca Pr o gcc A a t ac Ty r gct A a act Th r cca Pr o ac g Th r gt c ValI 115 gt g ValI aac As n ggt G31y ccc Pr o gca A a 1 95 gt c ValI gcc A a cct Pr o ct g Le u gt g ValI at c I Ie ac a Th r gga G31y aac As n tt c Ph e ct g Le u 1 65 gca A a t cc Ser at g vb t at c I Ie t ac Ty r ct g Le u aac As n at g vb t t cg Ser ggc G31y Ph e 135 cgc Ar g at c I Ie gct A a tt c Ph e ggc G31y G31y ct g Le u 40 at c I Ie at c I Ie ct c Le u gca A a ct g Le u 120 cca Pr a c aa O31n t cc Ser t cc Ser t cc Ser 200 ctt Le u gt a ValI c ag O31n cca Pr a gt g ValI at c I Ie ag c Ser 1 05 ggc G31y at c I Ie at c I Ie aac As n at g vb t 1 85 ggt G31y tt c Ph e 10 ggc G31y at g vb t at g vb t t cc Ser at g vb t 90 tt g Le u t gt CYS ac c Th r ggt G31y ct g Le u 1 70 ggt G31y gca A a cgt Ar g t cg Ser ct g Le u gt t ValI at g vb t aag Ly s 75 gtc ValI gct A a gca A a at g vb t gga G31y 1 55 t cc Ser g aa O31u t cc Ser g aa O31u aac As n tt t Ph e cat Hi s at c lIe act Th r ct t Le u ct c Le u at g vb t gt t ValI 140 gca A a g ac As p g aa O31u aac As n gca A a 220 cgc Ar g at g vb t ggt G31y ggc (31y G31y gcc A a at t I Ie c ag O31n 125 ggc O31y gt a ValI cga Ar g ggc G31y ct c Le u 205 at t I Ie aac As n at g vb t ct g Le u ct g Le u aag Ly s ct a Le u gga G31y 110 at t I Ie ac c Th r ggt G31y tt c Ph e gca A a 1 act Th r c aa O31n tt c Ph e ggc G31y aac As n at t I Ie t ac Ty r gt a ValI ct g Le u tt g Le u gcg A a t cc Ser ac c Th r 1 c aa O31n cca Pr a ct t Le u gt g ValI ac c Th r cca Pr a ggt G y aag Ly s ct g Le u t ac Ty r gt t ValI ac c Th r gca A a 1 g aa O3u t ac Ty r c ac Hi s t ct Se r ct t gt t gaa t ca ct t cca caa gca ct c Leu Val 03 u Ser Leu Pro O31n A a Leu 210 215 Page 289 tac aac gac Tyr Asn Asp 225 gt a gt c gcc Val Val Al a gaa acg cac O31u Thr Hi s seqI i st car r ect ed. t xt ct g aca cca at c t tc t tg gcg ct c acc ccg at c gca Leu Thr Pro I Ie Phe Leu Al a Leu Thr Pr o I Ie Al a 230 235 240 at c ct c ct c t tt t tc at c cgt gaa gat cac ct c aag I Ie Leu Leu Phe Phe Ilie Ar g G31u As p Hi s Leu Lys 245 250 255 t aat gacaca cgaaacttcc gt c <210O> 252 <21 1> 260 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 252 ValI Le u I Ie Thr Thr Tr p Le u Ph e Le u G31y 145 Le u As n Al a Le u Tyr 225 ValI Asp Pro Leu Thr Al a Val Al a Ty r [Vb t a3 n Al a G3 y Ser Thr Val Tyr Pr o Phe Ser Thr Leu 1 00 Phe Val Phe 115 Ilie Val O31n 130 Ser Asn Asn Ilie 31y G31y Val Pr o Al a 1 80 Ser Al a Nib t 1 95 Val O31u Ser 210 Asn Asp Al a Val Al a Al a Val Pr o Al a 03 y Pr o Thr Leu I'b t Val G31y 70 li1e G31y Thr Pr o O3 y Phe Asn Ser Phe Phe 150 Leu Phe 1 65 Al a Val Ser As p Leu Pro Leu Thr 230 1 1 e Leu 245 vb t I Ie Tyr Le u 55 As n vb t Ser G31y Ph e 135 Ar g I Ie Al a Ph e O31n 215 Pr o Le u G31y G31y Le u 40 li1e I Ie Le u Al a Le u 120 Pr o O31n Ser Ser Ser 200 Al a I Ie Ph e Le u ValI 25 G n Pr o ValI I Ie Ser 1 05 0Gy I Ie I Ie As n vb t 1 85 0Gy Le u Ph e Ph e Ser Le u ValI vb t Lys5 ValI Al a Al a vb t G31y 1 Ser (31u Ser (31u Al a 235 Ar g As n Ph e Hi s lIe Thr Le u Le u vb t ValI 140 Al a As p (31u As n Al a 220 Le u (31u Ar g vb t G y 0Gy 0Gy Al a I Ie O n 125 0Oy ValI Ar g 0Oy Le u 205 li1e Thr As p As n vb t Le u Le u Lys5 Le u O31y 110 I Ie Thr O31y Ph e Al a 1 Thr O31n Pr o Hi s Ph e O31y As n I Ie Tyr ValI Le u Le u Al a Ser Thr 1 O31n Pr o Le u I Ie Le u 255 Ou Thr His Ou Page 290 seqi i st car r ect ed. t xt 260 <210O> 253 <21 1> 1755 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1732) <223> RXN02926 <400> 253 ccct gacacc aat ct t ct t g gcgct caccc cgat cgcagt tt t tcat ccg t gaagat cac ct caaggaaa cgcacgaat a agt cgccgcg at cct cct ct atg aca cac gaa act 11! l'bt Thr Hi s O31u Thr t cc Ser cgc Ar g g aa O31u at g vb t ac a Thr ac c Thr ct c Le u at c I Ie t gg Tr p at g vb t 1 50 cgt Ar g gt t ValI ccc Pr o G y c aa O3n at g vb t gt c ValI t ac Tyr g ac As p ct g Le u 120 at c I Ie ct c Le u cgc Ar g ggc O3y cct Pr o gcg A a aaa Lys5 t cc Ser g aa O31u ct t Le u gt t ValI ggc G31y G31y c ag O31n at g vb t 1 70 ct a Le u gcc A a aag Ly s gt c ValI ct t Le u ct c Le u 75 gcc A a gga G31y t cc Ser cgt Ar g gca A a 155 ggt G31y ct c Le u g ac As p aag Ly s t gg Tr p g ac As p ggc G31y g aa O31u cgt Ar g gt c ValI gcc A a 140 at c I Ie gt c ValI ggt G31y gcg A a g aa G u t gg Tr p 45 c ag O3n G3y ac c Thr aaa Lys5 at c I Ie 125 gt a ValI at c I Ie at g vb t ggc G3y c ag O31n aaa Ly s gt c ValI at g vb t gt t ValI at c I Ie ggt O31y 110 ggc O31y c ag O31n gcg A a ggt O31y t gg Tr p 1 90 gt c ValI 15 act Thr ct c Le u at t I Ie g ac As p at g vb t 95 ct c Le u ggg O31y ggc O31y g ac As p gga O31y 1 75 tt c Ph e gca A a cct Pr o ag c Ser tt c Ph e c ac Hi s 80 ct g Le u tt c Ph e ct t Le u at c I Ie gt t ValI 1 60 gt c ValI ac c Thr 1 gga O31y t ca Ser gcg A a ggc O31y 65 at g vb t ccg Pr o at c I Ie gca A a ggt O31y 145 gt t ValI tt c Ph e g aa O31u g at As p t ca Ser ct g Le u 50 ac a Thr at g vb t at c I Ie gga O31y gga O31y 130 ggc O31y cca Pr a gga O31y gga O31y ac g Thr at g vb t 35 at g vb t gcc A a t gg Tr p t ac Tyr gcc A a 115 aat As n ggt O31y gca A a ct c Le u cca Pr a 1 aag Lys5 20 ac g Thr gt c ValI ct g Le u gt c ValI gga O31y 1 00 ct c Le u at g vb t gga O31y cgt Ar g t ct Ser 1 ggc O31y ct g Le u cct Pr a gcc A a cca Pr a at c I Ie aag O31y ac c Thr ct g Le u g aa O31u 1 gca A a t gg Tr p Page 291 seql i st cor r ect ed. t xt cgc Ar g gt c Val tgg Tr p 230 at c SIle at c SIle gtt Val ttc Phe ggt Gy 310 at g Met at g Met t cc Ser acc Thr ct g Leu 390 ct a Leu gcg Al a ggt Gy ttg Leu tgg gca ttc tgg atg Trp Ala Phe Trp Mbet aac atc cca ctg Asn lie Pro Leu gga atc atc gcc atc ggt G y Ile Ile Ala I e y 200 205 at t SIle t ac Tyr ttc Phe at t SIle gt g Val 280 aac Asn gca Al a cac Hi s gt c Val aca Thr 360 gt g Val cag G n at g Met gt c Val t ca Ser 440 acc Thr ttc Phe ggc Gy acc Thr 250 ct g Leu ct g Leu aac Asn ttc Phe at c SIle 330 at g Met aag Lys ct g Leu gga Gy gtt Val 410 t cc Ser gga Gy at g Met ct g Leu act Thr 235 tgg Tr p at c SIle cgc Ar g tt c Phe ggc Gy 315 aac Asn at g bet tac Tyr at c SIle at c SIle 395 ct g Leu gca Al a t cc Ser gaa G u gac Asp 220 ttc Phe ggt Gy at c SIle gca Al a acg Thr 300 at c SIle gcc Al a ggt Gy aaa Lys ttc Phe 380 t ac Tyr gt c Val acc Thr gcg Al a gaa G0u 460 at t SIle ttc Phe gga Gy acc Thr aaa Lys 285 ct c Leu at c SIle acc Thr acc Thr ct c Leu 365 ttt Phe ct c Leu ct g Leu gct Al a ct g Leu 445 aga Ar g cca Pr o at g bet t cc Ser acc Thr 270 gat Asp acc Thr ggc Gy gaa G u t cc Ser 350 ttc Phe gcc Al a ttc Phe at c SIle gt g Val 430 gt c Val at g Met aag Lys at c SI e cag G n 255 at c SIle cca Pr o acc Thr t ac Tyr gcc Al a 335 at c SIle cca Pr o cga Ar g gt c Val gtt Val 415 aac Asn ggt Gy cca Pr o aag Lys gtt Val 240 t ac Tyr gtt Val ttg Leu at t SIle ctt Leu 320 ggc Gy tgg Tr p cca Pr o at g Met ct c Leu 400 cag G n aac Asn ggc Gy gca Al a agc Ser 225 gcc Al a gag G u gcc Al a gtt Val gca Al a 305 ccg Pr o t ac Tyr act Thr at c SIle gaa G u 385 ggc Gy aac Asn ttc Phe at g Met 210 gt c Val gca Al a tgg Tr p gct Al a cca Pr o 290 ggc Gy acc Thr at g Met ggt Gy ggc Gy 370 gt g Val gt c Val acc Thr ttc Phe ttc Phe 450 ttc Phe agc Ser gat Asp 260 ct g Leu t cc Ser at c SIe ct c Leu at c SIe 340 cgc Ar g gt g Val acc Thr ct g Leu cca Pr o 420 caa G n ggc Gy 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 gcc atg gca caa ctt Ala IVet Ala G n Leu 465 Page 292 tca cca gaa gaa caa gcc gcc atg g Ser Pro O31u O31u O31n Ala Ala I'b t P 470 475 aac gaa ttg acg ccg gca atc gtc a Asn G31u Leu Thr Pr o Ala I Ie ValA 490 gat gcg t tc gcc ggt t cc t ac aac g Asp A a Phe A a 03 y Ser Tyr AsnA 5055 gt g at g at g cca ct g at c ggc at c g Val I'b t I'b t Pr o Leu Ilie G31y Ilie P 520 525 aag caa gaa aaa cta cgc gaa acc a Lys O31n O31u Lys Leu Ar g O31u Thr T 535 540 cct <210> 254 <211> 544 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn lIi st car r ect ed. t xt ca gcc caa ggc gga ctg gac tcc 1555 1l a A a 03 n 03 y 03 y Leu Asp Ser 480 485 at caa ttg cca acc gcg ctc cac 1603 6sn (31n Leu Pr o Thr Ala Leu Hi s 495 500 ac gca ct c at c cca gt g t tc t ac 1651 6sp Ala Leu Ilie Pr o Val Phe Tyr ~10 515 cg ct g ct t ct c t tg ct g t tt at t 1699 ~la Leu Leu Leu Leu Leu Phe Il e 530 cc aca gac taaacacaaa acaaatgaga 1752 hr Thr Asp 1 755 <400> 254 I'bt Thr Hi s O u Thr G31y Ser A a G31y vb t Pr o I Ie A a G31y 145 ValI Ph e O31u Thr vb t vb t A a Tr p Tyr A a 115 As n G31y A a Le u Pr o 1 95 Le u Pr o A a Pr a I Ie Lys5 G31y Thr Le u O31u 1 65 A a Tr p Ser Val Ar g Lys G31u (1n Ivb t b t Thr I Ie 70 Thr A a Leu 03 y I I e Phe Trp Leu 135 I'b t I Ie 150 Ar g G31y Val Leu Ar g Tr p G31y Ar g Lys5 A a G31y Le u Le u 1 li1e ValI Ser Tyr Pr a 1 Ph e Pr a 10 A a Lys5 Ser O31u Le u 90 ValI G31y G31y O31n vb t 1 70 Le u A a Lys5 ValI Le u Le u A a G31y Ser Ar g A a 1 G31y Le u A a (31u Tr p O31n G31y Thr Lys5 I Ie 125 ValI I Ie vb t (31y ValI Thr Le u I Ie As p vb t Le u O31y O31y As p O31y 1 Ph e Trp I'bt Asn Ilie Pro Leu O1y 205 Page 293 Ile Ile Ala Ile G y Val Ala II 210 215 seql i st cor r ect ed. t xt e Tyr Phe Leu Asp II 220 e Pro Lys Lys Ser 225 Al a G u Al a Val Al a 305 Pr o Tyr Thr SIle G u 385 Gy Asn Phe Met Al a 465 Gy Pr o SIle Leu Lys Thr Ser Al a 275 Met Leu Tyr Leu SIle 355 Met Ser Gy Leu Ar g 435 Val Al a Leu Al a Val 515 Leu Ar g Leu 245 Pr o Leu Phe Leu G n 325 Pr o SIle Val Thr Gy 405 Thr SIle Asn Leu Ser 485 Hi s Tyr SIle Asp Leu SIle Val G n 295 I le Val Met Asn Phe 375 Tr p Al a Val Ser Sy 455 Pr o G u Al a Met G n 535 Leu Thr Gy 265 G u Ar g bet Gy Gy 345 Gy Al a SIle G n Gy 425 Leu Leu G u Thr Al a 505 Pr o Lys Thr 235 Tr p SIle Ar g Phe Gy 315 Asn Met Tyr l Ie SIle 395 Leu Al a Ser G u Al a 475 Al a Ser SIle Ar g Phe Gy SIle Al a Thr 300 I le Al a Gy Lys Phe 380 Tyr Val Thr Al a G u 460 Al a l Ie Tyr Gy G u 540 Phe Gy Thr Lys 285 Leu SIle Thr Thr Leu 365 Phe Leu Leu Al a Leu 445 Ar g Met Val Asn SIle 525 Thr bet Ser Thr 270 Asp Thr Gy G u Ser 350 Phe Al a Phe SIle Val 430 Val bet Al a Asn Asp 510 Al a Thr SIe G n 255 I e Pr o Thr Tyr Al a 335 I e Pr o Ar g Val Val 415 Asn Gy Pr o Al a G n 495 Al a Leu Thr <210> 255 <211> 1294 <212> DNA <213> Corynebacteri um gl utami cum Page 294 seqi i st car r ect ed. t xt <220> <221 OIDS <222> (1294) <223> FRXA01319 <400> 255 ccct gacacc aat ct t ct t g gcgct caccc cgat cgcagt tt t tcat ccg t gaagat cac ct caaggaaa cgcacgaat a agt cgccgcg at cct cct ct atg aca cac gaa act l'bt Thr Hi s O31u Thr t cc Ser cgc Ar g g aa O31u at g vb t ac a Thr ac c Thr ct c Le u at c I Ie t gg T r p at g vb t 1 50 cgt Ar g gt t ValI cgc Ar g gt c ValI ccc Pr o G31y c aa O31n at g vb t gt c ValI t ac Tyr g ac As p ct g Le u 120 at c I Ie ct c Le u cgc Ar g ggc G31y gca A a 200 at t I Ie cct Pr o gcg A a aaa Lys5 t cc Ser g aa O31u ct t Le u gt t ValI ggc G31y G31y c ag O31n at g vb t 1 70 ct a Le u t gg Tr p tt c Ph e gcc A a aag Lys5 gt c ValI ct t Le u ct c Le u 75 gcc A a gga G31y t cc Ser cgt Ar g gca A a 155 ggt G31y ct c Le u at g vb t ct g Le u g ac As p aag Lys5 t gg Tr p g ac As p ggc G31y g aa O31u cgt Ar g gt c ValI gcc A a 140 at c I Ie gt c ValI ggt G31y aac As n g ac As p 220 gcg A a g aa O31u t gg Tr p 45 c ag O31n G31y ac c Thr aaa Lys5 at c I Ie 125 gt a ValI at c I Ie at g vb t ggc G31y at c I Ie 205 at t I Ie c ag O31n aaa Lys5 gt c ValI at g vb t gt t ValI at c I Ie ggt O31y 110 ggc O31y c ag O31n gcg A a ggt O31y t gg Tr p 1 90 cca Pr o cca Pr o gca A a cct Pr a ag c Ser tt c Ph e c ac Hi s 80 ct g Le u tt c Ph e ct t Le u at c I Ie gt t ValI 1 60 gt c ValI ac c Thr gga O31y 1 gga O31y t ca Ser gcg A a ggc O31y 65 at g vb t ccg Pr a at c I Ie gca A a ggt O31y 145 gt t ValI tt c Ph e g aa O31u at c I Ie g at As p t ca Ser ct g Le u 50 ac a Thr at g vb t at c I Ie gga O31y gga O31y 130 ggc O31y cca Pr a gga O31y gga O31y at c I Ie 210 ac g Thr at g vb t 35 at g vb t gcc A a t gg Tr p t ac Tyr gcc A a 115 aat As n ggt O31y gca A a ct c Le u cca Pr a 1 gcc A a aag Ly s 20 ac g Thr gt c ValI ct g Le u gt c ValI gga O31y 1 00 ct c Le u at g vb t gga O31y cgt Ar g t ct Ser 1 ggc O31y at c I Ie ct g Le u cct Pr a gcc A a cca Pr a at c I Ie aag Ly s ggc O31y ac c Thr ct g Le u g aa O31u 1 gca A a t gg Tr p ggt O31y aag aag agc gtc aag ttc cgc Lys Lys Ser Val Lys Phe Arg 225 Page 295 seql i st cor r ect ed. t xt tgg gat tac ctg ggc act Trp Asp Tyr Leu y Thr 230 235 atc ctg ttc acc acc tgg Ile Leu Phe Thr Thr Trp 250 atc atc att gga ctg atc Ile Ile Ile G y Leu Il e 265 gtt gtt gtg gaa ctg cgc Val Val Val G u Leu Arg 280 ttc caa aac cgc aac ttc Phe G n Asn Arg Asn Phe 295 ggt atc gca atg ttc ggc Gy Ile Ala IVet Phe G y 310 315 atg gtc cac gga atc aac IVet Val Hi s G y IIe Asn 330 atg atg gtc ggc atg at g IVet IVet Val G y IVt IVet 345 tcc aac aca gga aag tac Ser Asn Thr G y Lys Tyr 360 acc ttc gtg gca ctg atc Thr Phe Val Ala Leu Ile 375 ctg tgg cag atc gga at c Leu Trp n lie Gy lle 390 395 <210> 256 <211> 398 <212> PRT <213> Corynebacter i um gl <400> 256 Met Thr His G u Thr Ser 1 5 Sy Asp Thr Lys Leu Arg Ser Ser IVet Thr Pro G u Ala Leu bet Val Ala Mbet Sy Thr Ala Leu Pro Thr 70 IVet IVet Trp Val Ile Thr ttc ttc Phe Phe ggt G y at c SIle gca Al a acg Thr 300 at c SIle gcc Al a ggt Gy aaa Lys ttc Phe 380 t ac Tyr gga G y acc Thr aaa Lys 285 ct c Leu at c SIle acc Thr acc Thr ct c Leu 365 ttt Phe ct c Leu atg atc gtt Vet iIe Val 240 t cc cag t ac Ser G n Tyr 255 acc atc gtt Thr Ile Val 270 gat cca ttg Asp Pro Leu acc acc att Thr Thr Ile ggc tac ct t G y Tyr Leu 320 gaa gcc ggc G u Al a G y 335 t cc atc tgg Ser Ile Tr p 350 ttc cca cca Phe Pro Pro gcc cga at g Al a Arg IVet ttc Phe gcc gca acc Al a Al a Thr gag t gg t ct G u Tr p Ser gcc gct gca Al a Al a Al a 275 gtt cca at g Val Pro Mbet 290 gca ggc ct g Al a G y Leu 305 ccg acc tac Pro Thr Tyr tac atg ct g Tyr IVet Leu act ggt at c Thr G y Ile 355 atc ggc at g I e G y IVet 370 gaa gt g t cc G u Val Ser 385 agc Ser gat Asp 260 ct g Leu t cc Ser at c SIle ct c Leu at c SIle 340 cgc Ar g gt g Val acc Thr ct g Leu 245 cca Pr o ct g Leu ttc Phe ct g Leu cag G n 325 cca Pr o at c SIe gtt Val acc Thr 835 883 931 979 1027 1075 1123 1171 1219 1267 1294 ut ari cum Val Pro G y Lys G y Arg Sn G n Lys 40 vebt vet Al a I e Val y Al a Tyr Leu Pro Ala I 10 Al a Lys I Lys Val Ser Leu G u Leu 75 Leu Al a Page 296 Al a G u Tr p G n Gy Thr Val Thr Leu SIle Asp Met Al a Pr o Ser Phe Hi s Leu seqI i st car r ect ed. t xt Lys Leu 03 y Asp 90 Val G31y Ar g Lys Pr o I Ie A a G31y 145 ValI Ph e Ou le Ser 225 A a O31u A a ValI A a 305 Pr o Tyr Thr I Ie O31u 385 Tyr A a 115 As n G31y A a Le u Pr o 1 95 A a Ly s Thr Ser A a 275 vb t Le u Tyr Le u I Ie 355 vb t Ser G31y Thr Le u O31u 1 65 A a Tr p G31y Ar g Le u 245 Pr o Le u Ph e Le u O31n 325 Pr a I Ie ValI Thr Ph e Le u 135 li1e G31y Le u Tr p A a 215 As p Le u I Ie ValI O31n 295 li1e ValI vb t As n Ph e 375 Tr p Le u 120 li1e Le u Ar g G31y A a 200 li1e Tyr Ph e I Ie ValI 280 As n A a Hi s ValI Thr 360 ValI O31n Le u 1 05 li1e ValI Ser Tyr Pr a 1 85 Ph e Tyr Le u Thr G31y 265 (31u Ar g vb t G31y G31y 345 G31y A a I Ie Ser Ar g A a 1 55 G31y Le u vb t Le u Thr 235 Tr p I Ie Ar g Ph e G31y 315 As n vb t Tyr I Ie I Ie 395 ValI A a 140 li1e ValI G31y As n As p 220 Ph e Gy le A a Thr 300 li1e A a G31y Ly s Ph e 380 Tyr I Ie 125 ValI I Ie vb t Gy le 205 li1e Ph e G31y Thr Ly s 285 Le u I Ie Thr Thr Le u 365 Ph e Le u O3 y Leu Phe 110 o3 y 03 y Leu O31n G31y I Ie A a As p Val 1 O3 y 03 y Val 1 Tr p Phe Thr 1 Pr o Leu O31y Pr o Lys Lys I'b t I Ie Val 240 Ser 03 n Ty r 255 Thr I Ie Val 270 Asp Pro Leu Thr Thr Ilie O31y Tyr Leu 320 O3 u A a 03 y 335 Ser Ilie Tr p 350 Phe Pr o Pr o Ala Ar g I'b t Ph e <210O> 257 <21 1> 1510 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1487) Page 297 seqi i st car r ect ed. t xt <223> RXA01578 <400> 257 t gacct cccc t caaccact c act gaaaaga gaacact gat gt aaaacct c ggccgccaca gt cat accat t g at g at c b t I Ie gt cact tcct gt tcagccga gcc ct g ct g gt c gcg 11 Ala Leu Leu Val Ala gt a ValI ac c Thr c ag O31n cgt Ar g at t I Ie ac c Thr gt g ValI c aa O31n 120 ggc O31y Ph e ct c Le u aag Ly s tt g Le u 200 at t I Ie tt c Ph e gcc Al a g aa O31u gct Al a ggc O31y ac c Thr ct c Le u ct g Le u gcg Al a gt g ValI t cc Ser ct g Le u 1 70 g ac As p at g vb t gt g ValI aac As n c ag O31n g aa O31u tt c Ph e ct g Le u at t I Ie ct g Le u ct g Le u ct t Le u gcg Al a 140 tt c Ph e tt c Ph e ct t Le u tt c Ph e gt g ValI 220 g aa (31u ct c Le u ct t Le u acc Thr 45 at t I Ie gga G31y ggc G31y at c I Ie ct c Le u 125 ct t Le u t gg Tr p ag c Ser ggt G31y aac As n 205 ct g Le u aag Ly s aac As n aat As n gcg Al a ggt G31y t gt cys cgc Ar g at t I Ie 110 gga G31y gct Al a gt c ValI gt g ValI gt g ValI 1 90 gag O31u tt c Ph e cgc Ar g gcc Al a 15 gca Al a gcg Al a cgc Ar g gt t ValI ct g Le u 95 ct g Le u at t I Ie ggt G31y at g vb t aag Ly s 1 75 ct g Le u gcc Al a at c I Ie gt t ValI t cc Ser ac a Thr gcg Al a cgc Ar g gt c ValI at t I Ie cgc Ar g gt t ValI ggc G31y gct Al a 1 60 g aa O31u cca Pr o ggc G31y at c I Ie aag Ly s 240 at g gct Al a ct g Le u aaa Ly s 65 gct Al a c aa O31n c ag O31n ac c Thr t gg Tr p 145 gct Al a t cc Ser ct g Le u aaa Ly s ggt O31y 225 c ac Hi s ct g Le u gcc Al a tt t Ph e 50 gt g ValI gcc Al a ggt O31y c ag O31n t ct Ser 130 tt g Le u tt c Ph e ac c Thr gcg Al a ct c Le u 210 at c I Ie ccg Pr o gcg Al a c aa O31n 35 t cc Ser ct g Le u tt t Ph e gt t ValI gt a ValI 115 gt c ValI gct Al a t gc cys gct Al a gt g ValI 1 ggt O31y gcc Al a ct g Le u ccg Pr o 20 at c I Ie ct g Le u gt c ValI gct Al a gct Al a 1 00 ac c Thr aac As n g aa O31u gct Al a g aa O31u 1 t cc Ser gcc Al a gga O31y gt c ValI gca Al a ggc O31y Ph e ggc O31y ccg Pr o ggc O31y aat As n ggt O31y ac a Thr gt c ValI 1 g aa O31u at t I Ie gcg Al a gt c ValI agt Ser 245 ct g Le u at g vb t ct g Le u at g vb t aat As n cca Pr a g aa O31u ggt O31y ct t Le u 1 gct Al a ac c Thr gga O31y aac As n at c I Ie 230 gt t ValI Page 298 seqI i st car r ect ed. t xt t ac ct c ggt Tyr Leu G31y 250 caa cga cgc acc O31n Ar g Ar g Thr gca t tg ct g ct g Al a Leu Leu Leu ag C Ser 260 acc ct t ct c Thr Leu Leu aca at g Thr I'b t 265 acc ggt gt a tt c Thr 03 y Val Phe gcc Al a 270 gt a at g aat ggt Val [Vb t Asn G31y ct g ccc aac ct t Leu Pr o Asn Leu gcg Al a 280 cag gat gct gcc 03 n Asp Al a Al a ggt gcc ggc at g 03 y Al a 03 y [Vb t t ca Ser 290 gcg agc gt g gt g Al a Ser Val Val t gg t gg aca ct t Tr p Tr p Thr Leu ac c Th r 300 cca t at gcg ct g Pr o Tyr Al a Leu gct Al a 305 ggc t tg gt a tt c 03 y Leu Val Phe ggt cca G31y Pr o 310 at c gcc gga Ile Al a 03 y at t ggt at c Ilie 31y Ilie 330 ct c gcc gga aaa Leu Al a 03 y Lys tt t Ph e 320 gga t ac aag at c G31y Tyr Lys Ilie gt c ct g caa Val Leu O31n 325 acc t tc tt a Thr Phe Leu gct gcc acc at c Al a Al a Thr Ilie ggc gt t gcc gga G31y Val Al a G31y gcc Al a 340 gt c gga Val G31y 345 agc acc t cg cat Ser Thr Ser Hi s ct c Le u 350 gcg t ac ct c ggc Al a Tyr Leu G31y t cc at c t tc gt g Ser Ilie Phe Val ggt G31y 360 at t acc t at gca Il e Thr Tyr Al a at t gcc aac at c Il e Al a Asn Il e at g b t 370 ct c aac ggc ct g Leu Asn 03 y Leu 929 977 1 025 1 073 1121 1169 1217 1265 1313 1361 1409 1457 1 507 1510 at c gt g ct c t cc Ilie Val Leu Ser cct Pr o 380 gct aac aac caa Al a Asn Asn 03 n t at ct g cct ggc Tyr Leu Pr o G31y at g aac I'b t Asn 390 gca ggt gcc Al a 03 y Al a gca gtt tcc Al a Val Ser 410 aac ct a ggt gca Asn Leu 03 y Al a ggt G31y 400 at t t cc t tc gcc Ilie Ser Phe Al a at c ct c tt c Il e Leu Phe 405 gcc gca ggc Al a Al a 03 y acg gca ttc agt Thr Al a Phe Ser aac ggc gga gga Asn (1y (1y (1y t ac Ty r 420 at g t gg I'b t Tr p 425 gct ggc gt g at c Ala G31y Val Ilie at c Ilie 430 t tg gt c ct a gcc Leu Val Leu Al a ct c t gc t cc ct g Leu Cys Ser Leu ct g Le u 440 c ag O31n ag g at c cca cgc cca Ilie Pr o Ar g Pr o gct gaa gaa gcc Al a 03 u 03 u Al a 460 gaa (31u 445 t ca at c acc gat Ser Ilie Thr Asp ac a Thr 450 gt g gca gcc aaa Val Al a Al a Lys gct caa gcc gcc Al a 03 n Al a Al a t aaat ccaca aact gaact a <210> <211> <212> <213> 258 465 PmT Cor ynebact er i urn gi ut ani cumn <400> 258 krbt I Ie Al a Leu Leu Val Al a Val Phe Al a Phe (31n Leu Asn Al a Ser 1 5 10 Page 299 seqI i st car r ect ed. t xt I'bt Leu Ala Pro Ala Leu Ala Thr I'bt G3 u Thr (3 u Leu Asn A a Thr 25 A a Le u Ly s A a O31n O31n Thr Tr p 145 A a Ser Le u Ly s G31y 225 Hi s Le u As n vb t A a 305 G31y ValI Le u O31n Ser Le u Ph e ValI ValI 115 ValI A a CYS A a ValI 1 95 G31y A a Le u Le u Le u 275 A a Le u Ly s Gy le 355 G31y Ph e G31y Pr o G31y As n G31y Thr ValI 1 65 Ou le A a ValI Ser 245 Thr Pr o ValI Ph e ValI 325 Thr I Ie Thr Pr o vb t ValI Thr Ly s I Ie 135 G y A a Pr o Ser Tr p 215 Ph e 0 u Le u Le u Ser 295 Pr a 0On Le u ValI Thr Tr p ValI I Ie Pr a 1 05 Tyr O31y Ar g A a vb t 1 85 Le u Le u Tyr Le u vb t 265 O31n Tr p A a O31y O31y 345 li1e A a O31y Thr Le u 90 Le u A a ValI Ser Le u 1 70 As p vb t ValI As n O31y 250 Thr As p Thr Oy le 330 Ser Thr Ph e As p O31y 75 Ph e cys Le u As p I Ie 1 55 Pr a Tr p A a ValI I Ie 235 O31n O31y A a Le u I Ie 315 A a Thr Tyr Ph e Le u li1e Le u Le u Le u A a 140 Ph e Ph e Le u Ph e ValI 220 O31u Ar g ValI A a Thr 300 Le u A a Ser A a Thr lie O31y Oy le Le u 125 Le u Tr p Ser O31y As n 205 Le u Ly s Ar g Ph e As n 285 Pr a A a Thr Hi s O31y 365 A a O31y cys Ar g I Ie 110 O31y A a ValI ValI ValI 1 O31u Ph e Ar g Thr A a 270 O31y Tyr Oy le Le u 350 li1e A a Ar g ValI Le u Le u O31y Ly s 1 Le u A a I Ie ValI Tr p 255 ValI A a A a Ly s I Ie 335 A a A a A a Ar g ValI li1e Ar g ValI O31y A a 1 O31u Pr a Oy le Ly s 240 A a O31y Le u Ph e 320 O31y Ty r As n Ilie I'bt Leu Asn Oy Leu Oy lieVal Leu Ser Pro Ala Asn Asn On Page 300 seql i st cor r ec 370 375 Sy Tyr Leu Pro Gy IVet Asn Ala Gy Ala Phe 385 390 395 Ile Ser Phe Ala lle Leu Phe Ala Val Ser Thr 405 410 G y G y G y Tyr Al a Al a G y lVt Trp Al a G y 420 425 Leu Ala Phe Leu Cys Ser Leu Leu lie Pro Arg 435 440 Asp Thr Val Ala Ala Lys Val G n Ala G u G u 450 455 Ser 465 <210> 259 <211> 1470 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1447) <223> RXA02087 <400> 259 aatcggattc atgctgtgtg gtgtgatcag tttgctggct ccgggagacg ctgccaacgg cgaaagtcga gcaggtataa ted. txt 380 Asn Leu G y Al a y 400 Ala Phe Ser Asp Asn 415 Val Ile Ile Leu Val 430 Pro u Ser lie Thr 445 Ala Ala G n Ala Ala 460 gcggtcgcat ggatcttcgg gag G u ct c Leu caa G n gtt Val ggt Sy ttt Phe ttg Leu ccg Pr o agg Ar g ct g Leu gat Asp ttg Leu cgt Ar g ct c Leu gct Al a ccc Pr o tgg Tr p gac Asp ggt Sy act Thr gga Sy gcg Al a cgc Ar g agc Ser aaa Lys caa G n gcg Al a ttt Phe cag G n 75 tt g Leu gcg Al a at c SIle gct Al a tcg Ser agc Ser gcg Al a cga Ar g gcc Al a gt g Val att SIle ggc G y gt g Val aac Asn cca Pr o att SIle gt a Val ggc Sy 110 aag Lys gct Al a 15 gct Al a caa G n ct g Leu t at Tyr ttt Phe 95 ct g Leu tta Leu gt c Val gcg Al a ttg Leu ctt Leu 80 gca Al a ggc Sy at g Met 1 agt Ser gcg Al a gt g Val att SIle gcc Al a cca Pr o gga Sy acg cca att gtg Thr Pro Ile Val gtt Val ct g Leu tgg Tr p act Thr ggc Sy agc Ser tct Ser ct g Leu aag Lys t ca Ser cgc Ar g gct Al a gaa G u 100 ctt Leu ttt Phe att SIe gcg Al a ttg Leu gtg Val tgg Tr p aat Asn att ttc gcg cat gat cgt agg Ile Phe Ala His Asp Arg Arg Page 301 NO seql i st cor r ect ed. t xt 120 125 130 S gga gcc gcc acc ggg gtg tgg agt gct gtt gcc tca tca gct gga ctt 547 G y Ala Ala Thr G y Val Trp Ser Ala Val Ala Ser Ser Ala G y Leu ,C 135 140 145 4 ttc ggg cca gtt atc ggt ggt gtt ctg gtg ggg tgg atc agc tgg cgt 595 Phe G y Pro Val Ile G y G y Val Leu Val G y Trp Ile Ser Trp Arg 150 155 160 165 gct gtg ttc ttg gtt tat gtg ccg ctc gga ttg atc tcc cta ttt atg 643 Ala Val Phe Leu Val Tyr Val Pro Leu G y Leu I le Ser Leu Phe 'b t 170 175 180 00 gtg gcg cgt tat gtg cct aaa ctt ccc acg gga acc tcg aag atc gat 691 SVal Ala Arg Tyr Val Pro Lys Leu Pro Thr G y Thr Ser Lys I le Asp 185 190 195 .C tgg ctc tcg ggt gcg gt c tca ctt gtt gct gta ctt ggt gtg gtt ctt 739 STrp Leu Ser 3 y Al a Val Ser Leu Val Al a Val Leu G y Val Val Leu 200 205 210 gcc ttg cag cag ggg cca gaa ctt ggg tgg gga aca ctg att tgg gtg 787 Ala Leu n n O y Pro u Leu y Trp y Thr Leu Ile Trp Val 215 220 225 tcc ctt gcc gtt ggt att gct gca gct gtg ctcttt ata t ataggatg caa 835 Ser Leu Ala Val y I le Ala Ala Ala Val Leu Phe Ile Trp 'b t n 230 235 240 245 aca aga tcc aag gcg cca ctg atg ccg ttg agg att ttc aag acg cgc 883 Thr Arg Ser Lys Ala Pro Leu 'b t Pro Leu Arg Ile Phe Lys Thr Arg 250 255 260 aac ttc gcg atc ggt gca ttt tcg atc ttc agc ctg ggc ttt acg gtg 931 Asn Phe Ala le G y Ala Phe Ser Ile Phe Ser Leu G y Phe Thr Val 265 270 275 tat tcc gtt aat ttg ccc atc atg ttg tat ctg caa acg gct cag gga 979 Tyr Ser Val Asn Leu Pro Ile 'b t Leu Tyr Leu n Thr Ala n G y 280 285 290 atg tcg tcg cag ttg gcc ggt ttg atg ttg gtt ccg atg ggc atc atc 1027 'b t Ser Ser n Leu Ala y Leu 'b t Leu Val Pro b t G y Ile Ile 295 300 305 tct gtg gtg atg tca cca gta att gga cga ttg gtg gat cgc ctg gca 1075 Ser Val Val 'b t Ser Pro Val Ile G y Arg Leu Val Asp Arg Leu Ala 310 315 320 325 cca gga atg atc tcc aag atc gga ttc ggc gcg ctg att ttc tcg atg 1123 Pro G y Vbt Ile Ser Lys Ile y Phe y Ala Leu Ile Phe Ser [Vbt 330 335 340 gcg ttg atg gct gtc ttt a atatc gcc aac cta tcg ccg tgg tgg cta 1171 Ala Leu 'b t Ala Val Phe 'b t Ile Ala Asn Leu Ser Pro Trp Trp Leu 345 350 355 ctc atc ccg att att ttg ttc ggt agc tcc aac gcg atg agt ttt gca 1219 Leu Ile Pro Ile Ile Leu Phe G y Ser Ser Asn Ala 'b t Ser Phe Ala 360 365 370 ccg aac tct gtg att gct ctg cgt gat gtt ccg cag gat tta gtg ggc 1267 Pro Asn Ser Val Ile Ala Leu Arg Asp Val Pro n Asp Leu Val G y 375 380 385 tct gct tct ggt ttt tac aac acc tca cgc cag gtg ggc gct gtt ttg 1315 Ser Ala Ser y Phe Tyr Asn Thr Ser Arg n Val y Ala Val Leu Page 302 390 395 ggc gcc gct acc t tg ggc gct gt g a G31y Al a Al a Thr Leu G31y Al a Val 410 t cc t tc ggt gt t gcc at g ggt gcg g Ser Phe O31y Val Al a 'b t O31y Al a P 425 4 t ta at c t tt ggg t tc ct a gcg gt a a Leu I Ie Phe G31y Phe Leu Al a Val T 440 445 gatt t tct ca cga <210O> 260 <21 1> 449 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn I1 ist car r ect ed. t xt 400 405 .tg caa at a gga gt g ggc acg gt g /bt O31n Ilie 31y Va 1G31y Thr Val 415 420 ica at c ct g gt g aca ct c gt g ccc I a Ilie Leu Val Thr Leu Val Pr o .30 435 cc caa t tt aga t agt tgct cc Thr O31n Phe Ar g 1363 1411 1457 1470 <400> 260 I'bt Thr Pro I Ie Val Ser Al a ValI I Ie Al a Pr a G31y Al a Ser 145 Tr p I Ie Th r Le u Th r 225 G31y Pr a ValI G31y vb t I Ie Le u 115 As p Al a Ser Le u Ly s 1 95 ValI I Ie Ph e I Ie Al a Le u ValI Tr p As n Ar g Le u Ar g 1 65 vb t As p Le u ValI O31u Se r Ar g Leu Thr Leu O31n 1u Asp 40 Val Tyr Leu G31y Asp Ar g 70 Phe Thr Leu Leu Il e Al a Pro O31n Pr o 120 O3 y Al a Al a 135 Phe O31y Pr o 150 Al a Val Phe Val Al a Ar g Trp Leu Ser 200 Al a Leu 03 n 215 Ser Leu Al a 230 Al a Le u Le u Le u Ty r Al a Al a 1 05 Le u Th r ValI Le u Ty r 1 85 O31y O31n ValI Tr p 10 As p O31y Th r O31y Al a 90 Ar g Ser Oy le ValI 1 70 ValI Al a G31y Ly s O31n Al a Ph e O31n 75 Le u Al a I Ie ValI O31y 1 55 Ty r Pr a ValI Pr a Al a Ser Ser Al a Ar g Al a ValI I Ie Tr p 140 O31y ValI Ly s Ser O31u 220 Le u Le u Le u ValI As n cys O31n Hi s 125 Ser ValI Pr a Le u Le u 205 Le u O31y ValI As n Pr a I Ie ValI O31y 110 Ly s Al a Le u Le u Pr a 1 ValI O31y Al a Al a O31n Le u Ty r Ph e Le u I Ie ValI ValI O31y 1 Th r Al a Tr p Le u ValI Al a Le u Le u Al a O31y Ph e Al a O31y 1 Le u O31y ValI O31y Oy Ilie Ala Ala Ala Val Leu 235 240 Page 303 Phe I Ie Tr p I'bt O31n 245 seqI i st car r ect ed. t xt Thr Arg Ser Lys Ala Pro Leu I'bt Pr o Leu Ar g 255 I Ie Le u O31n Pr o 305 ValI Le u Ser A a O31n 385 ValI G31y ValI Ar g Ly s Ph e 275 A a G31y Ar g Ph e Tr p 355 Ser Le u A a G31y Le u 435 Ar g ValI G31y I Ie A a 325 vb t Le u A a G31y Le u 405 ValI Pr o Ph e Ser Ser 295 ValI G31y Le u I Ie As n 375 A a A a Ph e I Ie A a ValI 280 Ser ValI vb t vb t Pr o 360 Ser Ser A a G31y Ph e 440 I Ie 265 As n O31n vb t I Ie A a 345 li1e ValI O31y Thr ValI 425 (31y 250 O31y Le u Le u Ser Ser 330 ValI I Ie I Ie Ph e Le u 410 A a Ph e A a Pr o A a Pr o 315 Ly s Ph e Le u A a Tyr 395 O31y vb t Le u Ph e I Ie O31y 300 ValI I Ie vb t Ph e Le u 380 As n A a O31y A a Ser vb t 285 Le u I Ie O31y I Ie O31y 365 Ar g Thr ValI A a ValI 445 I Ie 270 Le u vb t O31y Ph e A a 350 Ser As p Ser vb t A a 430 Thr Ph e Tyr Le u Ar g O31y 335 As n Ser ValI Ar g O31n 415 li1e O31n <210O> 261 <21 1> 1338 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 OIDS <222> (1315) <223> RXA02088 <400> 261 agagcaccca agcgacat tg t tcgat aat a t gt tctat ta t tggt at taa t act ggt cag ct agat at aa aaggggcgca gggct agcct t gggt gt gaa at g t t a act caa aaa I'b t Leu Thr 03 n Lys 1 ata gaatta gag gct aaa cca aaa atc cca gag gag at c tgggt gct g I Ie (31u L eu (31u Ala Lys Pro Lys Ilie Pr o 31u 31u Ilie Tr p Val Leu 15 gt t gt ggct gcg t tt at t at t gcg ct gggc tat ggc ct gat t gcg ccg Val Val Ala Ala Phe Ilie lieAla Leu O31y Ty r O31y Leu Ilie Ala Pr o 30 Page 304 s ggt eql i st cor r ect ed. t xt ttt aat ata aat tt att ttg cca cag ttt gtg gtc lie Leu Pro n Phe Val Val agt Ser at g Met act Thr cag G n t cc Ser ccg Pr o ct g Leu 150 ttg Leu gct Al a cgg Ar g at t SIle ggt Gy 230 gca Al a gcg Al a t ca Ser gt g Val ggg Gy tta Leu t ac Tyr at g Met 120 gag Gu ggc Gy at g Met ctt Leu gct Al a 200 gat Asp tcg Ser gca Al a gcc Al a cgc Ar g 280 t cc Ser ttg Leu acc Thr cag G n acg Thr cgc Ar g at t SIle tgg Tr p 170 gt g Val agc Ser gcc Al a ttt Phe agc Ser 250 gga Gy ggc Gy ttt Phe gat Asp gct Al a ct g Leu tct Ser cgg Ar g 140 ggc Gy ttc Phe tgg Tr p agt Ser cgc Ar g 220 gt g Val ggc Gy gct Al a aaa Lys u y gcg Al a aag Lys at c l Ie ct g Leu gcc Al a 125 tgt Cys ccg Pr o gcg Al a cgg Ar g gt g Val 205 tcg Ser cgt Ar g gga Gy ttg Leu ccg Pr o 285 ggc Gy at c SIle acc Thr ctt Leu 110 at g Met tcg Ser gtt Val at t l Ie at g Met 190 ccg Pr o gcg Al a gt g Val gct Al a tgt Cys 270 at g Met gcc Al a ggt Gy acg Thr 95 cgt Ar g ggc Gy tcg Ser gt g Val t at Tyr 175 ccg Pr o gcg Al a ttg Leu gcg Al a at t l Ie 255 ct g Leu at t SIle ttc Phe Phe Asp Val Ser Ph t gca gct gcc e Ala Ala Ala cgg Ar g t cc Ser 80 ggg Gy ggc Gy ct g Leu gt a Val ggt Gy 160 ggt Gy aaa Lys ttg Leu ttt Phe gtt Val 240 gcg Al a caa G n at t SIle ggc G y ttg Leu cgt Ar g ctt Leu at c SIle at c SIle t at Tyr 145 gct Al a gct Al a acc Thr cgc Ar g agt Ser 225 ctt Leu ggt G y ttc Phe t cc Ser aca Thr 305 ttg Leu cgt Ar g gtt Val gca Al a gt g Val 130 gcc Al a gcg Al a t cc Ser aac Asn ttt Phe 210 gct Al a cca Pr o ttt Phe gcg Al a ggg G y 290 gaa G u ttt Phe gt g Val gcg Al a ggt G y 115 aag Lys agt Ser at g Met gtt Val gat Asp 195 gct Al a ttt Phe ct g Leu gcc Al a ggc G y 275 ct g Leu gt g Val gcg Al a t at Tyr ttg Leu 100 at t SIle at g Met tcg Ser t cc Ser ggc Sy 180 t ca Ser gag G u gcc Al a ttt Phe at g Met 260 gat Asp at c SIle tgg Tr p ccg Pr o ct c Leu gcg Al a ggt G y gcg Al a ttc Phe ggt Sy 165 tta Leu ctt Leu gca Al a aat Asn gcc Al a 245 gct Al a ct c Leu gt c Val at c SIe 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 aat gca gtg ttc acg gca atg atc gga Asn Ala Val Phe Thr Al a IVet I e y Page 305 seqIi ct g at c acg gt a t ct gcg t tg gca ggt Leu I Ie Thr Val Ser Al a Leu Al a G31y 310 315 cca agt cag cag gcg gt g ct C gct gat Pr o Ser (31n O31n Al a Val Leu Ala Asp 330 gga aaa gt c t ta gcg aat t tc caa at g G3 y Lys Val Leu Al a Asn Phe G3 n I'b t 345 350 gt t ggc ccg at t ct c gt a ggc at g at c Val G31y Pr o Ilie Leu Val G31y 'b t Ilie 360 365 at c gga t tc at g ct g t gt ggt gt g at c Ilie G31y Phe I'b t Leu Cys G31y Val Ilie 375 380 tgg atc ttc ggc cgg gag acg ctg cca Trp Ilie Phe G31y Ar g (31u Thr Leu Pr o 390 395 t aaat gacgc caat tgt gga gt c <210> 262 <211> 405 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 262 I'bt Leu Thr O31n Lys I Ie O31u Leu O31u 1 5 O31u I Ie Tr p Val Leu Val Val Ala Ala 25 G31y Leu I Ie Al a Pr o Ilie Leu Pro O31n 40 Ser Phe Ala Al a Al a Se r Al a Val Val Leu Leu Phe Al a Pr o I'b t Ser O31y Ser 70 Ar g Ar g Val Tyr Leu Thr G31y Leu Leu Leu Val Al a Leu Al a O31n O31u Tyr Tr p 100 105 Ilie Al a G31y Ilie G31y Ser Thr I'b t Phe 115 120 Ilie Val Lys I'b t Al a Pr o Val O31u Ilie 130 135 Tyr Al a Ser Ser Phe Leu Phe 03 y Asn 145 150 Al a Al a I'b t Ser G31y Leu G31y I'b t Ar g 1 65 st cor r ect ed. t xt gct ggt gcg ggc Al a 03 y Al a 03 y 320 gt t at a gat t cc Val Ilie Asp Ser 335 gcg cag gat ttc Al a 03 n Asp Phe gca gaa cag gca Al a 03 u 03 n Al a 370 agt t tg ct g gct Ser Leu Leu Al a 385 acg gcg aaa gt c Thr Al a Lys Val 400 t tg Le u cgc Ar g ggt G31y 355 ggC G31y gcg Al a gag O31u ct t Le u CCC Pr o 340 gcg Al a tt c Ph e gt c ValI c ag O31n aat As n 325 ggC (31y at t I Ie c aa O31n gca Al a gt a ValI 405 1 075 1123 1171 1219 1267 1315 1338 Al a 10 Ph e Ph e Ser Le u Thr 90 O31n Thr Ar g I Ie Tr p 1 70 Ly s I Ie ValI I Ie I Ie 75 ValI I Ie ValI O31y I Ie 1 55 Pr o Ly s Al a O31y Al a Ly s I Ie Le u Al a 125 Cys Pr o Al a I Ie Pr o O31u Leu 03 y Tyr Phe Asp Val O31y Al a Ar g Il e 03 y Ser Thr Thr 03 y Leu Ar g (31y 110 I'b t O31y Leu Ser Ser Val Val Val O31y 1 Il e Tyr 03 y 1 krbt Pr o Lys 1 Al a Se r ValI Leu Ala Ala Leu Val Val Trp Trp Arg 1 85 Page 306 seqI i st car r ect ed. t xt Thr Asn Asp Ser Leu Arg Lys Al a Asp Ser 195 200 Asn Ser Val Pro Al a Leu 205 Ar g Ser 225 Le u G31y Ph e Ser Thr 305 Al a As p As p O31n Le u 385 Ly s Al a Ph e Le u Al a G31y 275 Le u ValI Le u Ar g G31y 355 G31y Al a aOu Al a As n Al a 245 Al a Le u ValI I Ie As n 325 G31y I Ie O31n Al a ValI 405 I Ie G31y 230 Al a Al a Ser As n Le u 310 Pr o G31y ValI I Ie Tr p 390 Ly s 215 Tr p Al a Ph e As p Al a 295 li1e Ser Ly s G31y G31y 375 li1e As p Ser Al a Al a Ar g 280 ValI Thr G n ValI Pr o 360 Ph e Ph e Ser As n Ph e Al a 265 li1e Ph e ValI G n Le u 345 li1e vb t 0Gy Al a Ph e Ser 250 G31y G31y Thr Ser Al a 330 Al a Le u Le u Ar g Tyr G31y 235 As n As n Ar g Al a Al a 315 ValI As n ValI cys O31u 395 Ar g 220 ValI G31y Al a Ly s vb t 300 Le u Le u Ph e G31y G31y 380 Thr Ser Ar g G31y Le u Pr o 285 li1e Al a Al a O31n vb t 365 ValI Le u Al a ValI Al a cys 270 vb t G31y G31y As p vb t 350 li1e I Ie Pr o Le u Al a I Ie 255 Le u I Ie Ph e Al a ValI 335 Al a Al a Ser Thr <210O> 263 <21 1> 1239 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (1216) <223> RXA00764 <400> 263 t act gcgt cg gat ccgct ga t gct t gcaga at cggacagt gat gggccgt ct gcgcct gc acct gggacg act ggat tat t aggggt gga att t tcgct c at g aca ctc aag act 115 I'b t Thr Leu Lys Thr 1 agc gt t t tg gca ct a ct c t ta gat aac gt g cat gt t ct t ct g at t gcg 163 Ser Val Leu Ala Leu Leu Leu Asp Asn Val His Val Leu Leu Ile Ala 15 aat cct gag t cg acc acg cag acg cag aaa ct t t tc cgt cgt gt g gt g 211 As n Pr o (3u Se r Thr Th r (31n Th r O31n Lys Leu Phe Ar g Ar g Val Val 30 Page 307 seql i st cor r ect ed. t xt cct Pr o cac Hi s gat Asp gt g Val gag G u ttt Phe gcc Al a ggt Gy 150 gct Al a aga Ar g ctt Leu gag G u at g Met 230 cct Pr o ttt Phe cac Hi s aag Lys ttg Leu gga G y gat Asp aat Asn ttg Leu cgt Ar g 120 gt g Val tgg Tr p ttt Phe ttc Phe gcg Al a 200 gt g Val ctt Leu gt g Val ttg Leu at t SIle 280 acc Thr gcg Al a cat Hi s at t SIle tta Leu gcg Al a ctt Leu ttg Leu ggt Gy at t SIle 170 ttt Phe gt g Val agc Ser tcg Ser aat Asn 250 agt Ser gt g Val gct Al a ctt gat ggt gtg tcg ctt Leu Asp y Val Ser Leu 45 gcg gag gaa atg gtt gcg Ala u G u vet Val Ala atc ccc gcg ggt ggg gac lie Pro Ala G y y Asp 75 ctt ggg tcg gcg gaa ggt Leu G y Ser Al a G u G y 95 att gcg gtg ttg cca acg lIe Ala Val Leu Pro Thr 110 ggt tac ccc act gac ccg G y Tyr Pro Thr Asp Pro 125 att cgg aag aac cac acc Ile Arg Lys Asn His Thr 140 gat gat cag ggg act cgt Asp Asp G n y Thr Arg 155 160 gat gcg gat gtt att gcc Asp Ala Asp Val Ile Al a 175 gcg gct tca ccg ttg ttg Ala Ala Ser Pro Leu Leu 190 aaa act cag att aag cca Lys Thr n Ile Lys Pro 205 aaa ggg cac aaa ttg caa Lys G y His Lys Leu G n 220 aat acc aat ccg tgg act Asn Thr Asn Pro Trp Thr 235 240 ccg cag aat tct ttt gac Pro G n Asn Ser Phe Asp 255 gtg cga gga ttc ggg gga Val Arg Gy Phe Gy y 270 ggg cat ggt cgg aag ttg G y His Gy Arg Lys Leu 285 ttt gat gat gcg gag aaa Phe Asp Asp Ala G u Lys 300 gaa gcg G u Al a ggt ttg G y Leu ggc acc G y Thr gat ttt Asp Phe ggg tct G y Ser tat gct Tyr Al a 130 aga act Arg Thr 145 tgg ttc Trp Phe agg gt c Arg Val tat ct g Tyr Leu ccg aaa Pro Lys 210 aaa gag Lys G u 225 ttt ttg Phe Leu aca ggt Thr G y gtg gca Val Ala gag aag G u Lys 290 agg ttt Arg Phe acg gtg Thr Val gtc aac Val Asn aga aac Arg Asn 100 gcc aat Al a Asn 115 gcc gct Al a Al a atc acc Ile Thr gcg gtt Al a Val gaa cgg G u Arg 180 cag gtg G n Val 195 att acc Ile Thr gaa gt g G u Val ggt ccg G y Pro ctg ggg Leu G y 260 gcg atg Al a Vet 275 ttg atc Leu II e 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 gta acg ctc acg tgc Val Thr Leu Thr Cys 305 Page 308 seqI i st car r ect ed. t xt gac agc gat cag cgt t t c caa gt t gat ggt gag t at gaa Asp Ser Asp O31n Ar g Phe O31n ValI Asp O31y O31u Tyr O31u 310 315 320 aca aag gt g gt g t tg gaa t ca at c act gat gcg gt g cga Thr Lys Val Val Leu O31u Ser Ilie Thr Asp Ala Val Ar g 330 335 ccg aag acg cat ccg aca ccg ccg atc atg aat tgg gct Pr o Lys Thr Hi s Pr o Thr Pr o Pr o Ilie I'bt Asn Tr p Ala 345 350 t tt aag cac gt c cgt gat t tc ct c cgg gt g cgc acg tt t Phe Lys Hi s Val Ar g Asp Phe Leu Ar g Val Ar g Thr Phe 360 365 370 t aggat tcat cggagtt t tc tt c <210> 264 <211> 372 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn ggc aaa 03 y Lys gt g t at Val Ty r 340 gt c cat Val Hi s 355 ggc at c O31y I Ie 1 075 1123 1171 1216 1239 <400> 264 I'bt Thr Leu Lys Val Leu Leu Ilie Phe Ar g Ar g Val O31u Ala Ar g Ph e O3 y Leu Thr Val O31y Thr Val Asn Asp Phe Arg Asn 1 00 (3 y Ser A a Asn 115 Tyr A a A a A a 130 Ar g Thr Ilie Thr 145 Tr p Phe Ala Val Ar g Val O31u Ar g 1 80 Tyr Leu 03 n Val 1 95 Pr o Lys Ilie Thr 210 Thr A a ValI Thr As p O31u Le u ValI As p Le u As n 1 65 A a Ser ValI Ser As n Pr o Hi s As p 70 ValI O31u Ph e A a O31y 150 A a Ar g Le u O31u ValI Pr o A a Tyr 55 Ph e I Ie As p A a Le u 135 Thr O31y Ser Ar g A a 215 Le u O31u Le u 40 O31y As p As n Le u Ar g 120 ValI Tr p Ph e Ph e A a 200 ValI A a Ser 25 vb t O31y ValI O31y Pr o 1 05 A a O31u Lys5 O31y O31y 1 85 Tr p As p Le u 10 Thr A a Hi s I Ie Le u 90 A a Le u Le u Oy le 1 70 Ph e ValI Le u Thr Le u A a I Ie 75 Le u I Ie O31y I Ie As p 1 55 As p A a Lys5 Le u O31n As p O31u Pr a G31y A a Tyr Ar g 140 As p A a A a Thr As p Thr G31y O31u A a Ser ValI Pr a 125 Lys5 O31n As p Ser O31n 205 As n O31n ValI vb t O31y A a Le u 110 Thr As n O31y ValI Pr a 1 li1e ValI Lys5 Ser ValI O31y O31u Pr a As p Hi s Thr I Ie 1 Le u Lys5 Hi s Le u Le u A a As p O31y Thr Pr a Thr Ar g 1 A a Le u Pr a Ser Lys O31y 220 Hi s Lys Leu 03 n Page 309 seql i st cor r ec Lys G u G u Val Pro IVet Leu Leu Ala Ser Asn 225 230 235 Phe Leu y Pro Leu Pro Val Val Thr Asn Pro 245 250 Thr G y Leu G y Leu Phe G y Leu Thr Ser Val 260 265 Val Ala Ala Met Met His Leu IIe G y Val G y 275 280 G u Lys Leu lie Ala Lys Arg Thr lie Ala Phe 290 295 Val Thr Leu Thr Cys Asp Ser Asp G n Arg Phe 305 310 315 Tyr G u y Lys Pro Thr Lys Val Val Leu G u 325 330 Val Arg Val Tyr Ala Pro Lys Thr His Pro Thr 340 345 Trp Ala Val His Leu Phe Lys His Val Arg Asp 355 360 Thr Phe Gy lie 370 <210> 265 <211> 271 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(271) <223> RXN03125 <400> 265 tgacaccggc gcgacgtatg gcattactgg cgtcacccca tcgcctcggc gacgtcctgg ttcct t acgt tctgatcgt t ted. txt Thr Asn Sn Asn Arg G y Hi s G y 285 Asp Asp 300 G n Val Ser Ile Pro Pro Phe Leu 365 Tr p Phe 255 Sy Lys G u Sy Asp 335 Met Val ctc gtg ct g Leu Val Leu gct ctg ggc Al a Leu G y gcg atc ttc Al a li e Phe ctg ctg tgc Leu Leu Cys <210> 266 <211> 57 <212> PRT ttg ctc gtg ttc cgg Leu Leu Val Phe Ar g ttt ggc ttg tca gtt Phe G y Leu Ser Val caa gaa ggt gct ttc Sn G u y Al a Phe 45 ttc Phe tcc att tgg Ser I e Tr p 15 ct g gct acc Leu Al a Thr 30 ggc atc at c Sy lie l ie atttacgatg acatctctgc ttg gtt cta gcg ttc Leu Val Leu Ala Phe 1 gtc cca ttg atc gcg Val Pro Leu Ile Ala ttt ggt gct acc gtg Phe G y Al a Thr Val gac gat cct cag cca Asp Asp Pro G n Pro Page 310 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ai cum <400> 266 Leu Val Leu Ala Phe Leu Val Leu Leu Leu Val Phe Arg Ser lie Trp 1 5 10 Val Pro Leu I e Ala Ala Leu Gy Phe Gy Leu Ser Val Leu Ala Thr 25 Phe G y Ala Thr Val Ala lle Phe Gn Gu Gy Ala Phe Gy Ile Ile 40 Asp Asp Pro G n Pro Leu Leu Cys Phe <210> 267 <211> 1443 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1420) <223> RXN01553 <400> 267 atgatgatgt cctcagcaag tccaagcgcc aagccatgct ggaaacaatt ctcgagctga taccaagcca gacttaaatt tctaccttaa agtcttgagc ttc Phe gcg Al a ttt Phe gag Gu ggc G y at c SIle gt g Val ttt Phe gag G u cgc Ar g tgg Tr p ttc Phe t cc Ser at t SIle ggc G y ttg Leu tgg Tr p 120 gct Al a acc Thr tgg Tr p gt c Val gcc Al a gcg Al a cgc Ar g ttt Phe ggt Sy gtt Val aaa Lys ggt Gy t at Tyr aca Thr gtt Val 75 cgc Ar g tgt Cys gt a Val cag G n ccc Pr o t cc Ser ct c Leu t ca Ser gtt Val agg Ar g ct c Leu gcc Al a t ac Tyr 140 aca Thr gcc Al a act Thr 45 ct g Leu gcc Al a t ca Ser ttt Phe at c l Ie 125 t at Tyr cca Pr o tct Ser gat Asp t at Tyr cca Pr o ct g Leu aca Thr 110 at g Met Wt gcg Al a aaa Lys 15 ttc Phe t ca Ser tcg Ser gt c Val cgc Ar g 95 gt a Val gcg Al a ccc Pr o aac Asn gt c Val at g Met at g Met 80 at g Met aag Lys at c l Ie at g act Met Thr 1 cca att Pro Ile gcg gtc Al a Val ggc gcc G y Al a gcg gtc Al a Val ggc agg G y Arg tgg aca Trp Thr aac act Asn Thr gcc aac Al a Asn 130 gtt cag gaa Val Gn Gu gtt tct tgg Val Ser Trp ct c gt g acc Leu Val Thr acc ctc ccc Thr Leu Pro gcc at c gct Al a I e Al a cga t ca gat Arg Ser Asp ctt gtc acc Leu Val Thr 100 gat ccc aca Asp Pro Thr 115 atc acc ttt Ile Thr Phe cag ctc tcc caa atc tcg acc G n Leu Ser G n I e Ser Thr 145 Page 311 seql i st cor r ect ed. t xt cgc Ar g 150 ttc Phe ggt Sy at g Met tct Ser gct Al a 230 ttt Phe ttc Phe acc Thr gat Asp gca Al a 310 at c SIle ttc Phe gcg Al a ctt Leu acc Thr 390 gt g Val aac Asn ggc G y ggc G y at c SIle 200 at t SIle gaa Gu cag G n at c SIle ggt Gy 280 ct c Leu ct c Leu at t SIle gaa Gu gt g Val 360 cca Pr o cgt Ar g ct c Leu ggc Gy gtt Val 170 acc Thr ct c Leu gca Al a cac Hi s gct Al a 250 gca Al a at c SIle ttc Phe gga Gy ctt Leu 330 ccc Pr o cct Pr o ggc G y gt g Val ggc G y 410 cga gt t t ct Arg Val Ser 155 cta ctg ct t Leu Leu Leu cgc gga ttc Ar g G y Phe gt c gca gt g Val Al a Val 205 ctt ctt cga Leu Leu Arg 220 ccc aaa ggc Pro Lys G y 235 gag ctg tgg G u Leu Trp act gtt ttc Thr Val Phe ctt gcg gtc Leu Al a Val 285 ggt gtc gca Sy Val Ala 300 ttc cta gac Phe Leu Asp 315 gcc atc atg Al a lle Iet act aat ttc Thr Asn Phe gca cag tca Al a G n Ser 365 cag gaa ggc Gn Gu y 380 agt tgg at g Ser Trp IVet 395 gat gac cgc Asp Asp Arg ggt Gy gtt Val ct a Leu 190 ctt Leu gt c Val ct c Leu aaa Lys cgt Ar g 270 tct Ser gcc Al a gat Asp at c SI e tgg Tr p 350 gcg Al a cag G n gt g Val act Thr ttc ggc tgg tcc Phe y Trp Ser 160 tgt tac cta ggt Cys Tyr Leu y 175 aac ctg ccc atc Asn Leu Pro lle gca gcc gtt tgg Ala Ala Val Trp 210 cca gaa att gag Pro u lie u 225 ata gct gcc tac I e Al a Al a Tyr 240 caa gac cgc aac G n Asp Arg Asn 255 gac gga ctc gcc Asp G y Leu Al a gtg tac gga ct a Val Tyr G y Leu 290 aac gtg gtc t ct Asn Val Val Ser 305 cgc gtc ggg cca Arg Val y Pro 320 gcc gat gct gca Al a Asp Al a Al a 335 atc ttc gga tta I e Phe G y Leu tcg aga agc tat Ser Arg Ser Tyr 370 ctc ttc ggc ctt Leu Phe G y Leu 385 ccg tcg ctg ttt Pro Ser Leu Phe 400 ggt att ttg gcc G y Ile Leu Ala 415 Page 312 at g Met tt t ttt Phe gaa G u 195 ttc Phe gca Al a aag Lys t cc Ser gga Gy 275 tct Ser gcg Al a aaa Lys at t l Ie at c SIle 355 tta Leu t at Tyr ggt G y at c l Ie ggt Gy gtt Val 180 gac Asp ttg Leu cag G n gat Asp gt g Val 260 gt a Val gcc Al a ttg Leu ccc Pr o ct c Leu 340 ct c Leu aca Thr gcc Al a gt a Val gcg Al a 420 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 seqi i st car r ect ed. t xt at t ct g ct a t tc ggt at t gt g ct g ct g agc atg9 gt g aag cca ccg cac I Ie Leu Leu Phe G31y I Ie Val Leu Leu Ser l'bt Val Lys Pro Pro Hi s 425 430 435 aag gtg aag tagacaaagc gcccacaagg att Lys Val Lys 440 <210> 268 <211> 440 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1411 1443 <400> 268 l'bt Thr Val G3 n G3 u Ph e As p Pr a A a G y A a G3y Tr p As n A a Ser 145 Tr p Le u Pr a ValI I Ie 225 A a Ar g ValI Le u Thr A a Ar g Le u As p 115 le le vb t Ph e (31u 1 95 Ph e A a Lys5 Ser Tr p Thr Pr a A a As p Thr Thr Ph e Thr Tyr 1 65 A a G31y ValI ValI Le u 245 Tyr A a Ph e G u G3y 70 li1e ValI Ph e (3u Ar g 150 Ph e G3y vb t Ser A a 230 Ph e Ph e Ph e I Ie G y ValI Lys5 Ph e Ph e Ph e 135 Ou Gy As p As n A a 215 A a G3y Le u A a As p Ser As n ValI Thr vb t 1 Ph e (31u ValI I Ie As p 1 Ar g Pr a As p I Ie A a 265 Thr 10 Tr p ValI A a A a Ar g 90 Ph e G31y ValI G31y ValI 1 70 Thr Le u A a Hi s A a 250 A a Lys5 G31y Tyr Thr ValI 75 Ar g cys ValI O31n Ar g 1 55 Le u Ar g ValI Le u Pr a 235 (31u Thr Pr a Ser Le u Ser ValI Ar g Le u A a Tyr 140 ValI Le u G31y A a Le u 220 Lys5 Le u ValI Thr A a Thr Le u A a Ser Ph e I Ie 125 Tyr Ser Le u Ph e ValI 205 Ar g G31y Tr p Ph e Lys5 Ph e Ser Ser ValI Ar g ValI A a O31n Ph e cys 1 As n A a Pr a I Ie O31n 255 As p Pr a As n ValI vb t vb t vb t Ly s I Ie Le u G31y 1 Tyr Le u A a (31u A a 240 As p G31y Leu Ala G31y Val Phe Thr Phe G31y Ala I Ie Leu Ala Val Ser Val Tyr Page 313 Gy Val 305 Gy Al a Gy Ser Gy 385 Leu Leu Val 275 Ser Al a Lys l Ie SIle 355 Leu Tyr Gy SIle Pr o 435 Gy Gy I e 325 Phe Cys Ar g Thr Phe 405 Leu Hi s Asp Al a 310 I le Phe Al a Leu Thr 390 Val SIle Lys Val 295 Leu Leu Val Phe Ser 375 Gy Gy Leu Val seql i st cor r ect ed. t xt 280 285 Leu Leu Phe y Val Ala 300 Leu G y G y Phe Leu Asp 315 lie Ser Leu Ala lie IVet 330 G u y Pro Thr Asn Phe 345 Val Gy Pro Ala G n Ser 360 365 Pro Asp Gy n u y 380 Arg Ala Val Ser Trp 'bet 395 Leu Thr Gy Asp Asp Arg 410 Leu Phe Gy Ile Val Leu 425 Lys 440 Al a Asp SI e Tr p 350 Al a G n Val Thr Leu 430 Asn Ar g Al a 335 I e Ser Leu Pr o Gy 415 Ser <210> 269 <211> 840 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(817) <223> RXN00535 <400> 269 aatcgcatgg ggcaccgtgg tcagacaccg gat cctaaggagc tcacctttac tcaatgctct gat at g Met ttc Phe ttc Phe act Thr at c SIle acc Thr acc Thr ct g Leu at c SIle ttg Leu at g Met ttg Leu ct c Leu tgg Tr p cgc Ar g ct g Leu gt a Val t ca Ser gct Al a ccc Pr o t at Tyr caa G n cga Ar g 45 ct c Leu ccc Pr o ttc Phe cca Pr o ttc Phe ct c Leu gga Gy cgcgctc cgcaccccaa aagatggctc gacaccg atg tgg tgg gca ggc 115 IVet Trp Trp Ala Gy 1 tta caa aca gta gca ctt ggt 163 Leu G n Thr Val Ala Leu y 15 gtg ctt gtc ctg tcg ctg atg 211 Val Leu Val Leu Ser Leu IVet aat ggc tac cga cta cgc cga 259 Asn Gy Tyr Arg Leu Arg Arg acc gta gcc gtg ggc atc atg 307 Thr Val Ala Val Gy Ile Mat aac ccc cac ccc cca ctc gat 355 Asn Pro His Pro Pro Leu Asp Page 314 IND seqI i st car r ect ed. t xt 75 80 Scga tgg at t cca gta ctt t ta gt cggc gt t gca gt aat gggt gga atg 403 Ar g Tr p I Ie Pr o Val Leu Leu Val O31y Val Al a Val 'b t O31y O31y Ib t 95 100 t tgg ct gctt gcg gaa tac gt a t taaag aag gac aaagcc ct cat cct t 451 Tr p Leu Leu Al a O31u Tyr Val Leu Lys Lys Asp Lys Al a Leu Ilie Leu c~1105 110 115 ggt ct t gt gacg ggt gcat tg t tt ggc tac gt agca gt gat g tccaaa 499 G31y Leu Val Thr G31y Al a Leu Phe G31y Tyr Val Al a Val I'b t Ser Lys 120 125 130 00 gcc gcg gt ggat ct t t tt gt ccat caa ggc at aacg gga ct cat ct tg 547 SAl a Al a Val Asp Leu Phe Val His O31n 31y Ilie Thr G31y Leu Ilie Leu 135 140 145 .C aac tgg gaa ggc tac ggc ct aat cct cacc gca t tactt gga aca at c 595 SAsn Tr p O31u 31y Tyr G31y Leu Ilie Leu Thr Al a Leu Leu G31y Thr Ilie S150 155 160 165 gt gcag cag tat t cc t tt aac gct ggc gaa ct acaa aaa tcg ct accc 643 Val O31n O31n Tyr Ser Phe Asn Al a 31y O31u Leu O31n Lys Ser Leu Pr o 170 175 180 gcc at g acc at t gcc gaa cca at t gt t gcc t tc agt t tg ggc tac t tg 691 Al a I'bt Thr Ilie Al a O31u Pr o Ilie Val Al a Phe Ser Leu G31y Tyr Leu 185 190 195 gt t ct g ggc gaa aaa t tc caa gt c gt g gac t gg gaa t gg at c gcc at g 739 Val Leu G31y O31u Lys Phe O31n Val Val Asp Tr p O31u Tr p Ilie Al a N/b t 200 205 210 ggc at c gca ct a ct g gt g at g at t gt t t cc acc at t gca ct g t ct cgt 787 O31y Ilie Al a Leu Leu Val N/b t Ilie Val Ser Thr Ilie Al a Leu Ser Ar g 215 220 225 aca agc aca atg ccg gcc gga tcg aaa agg taaaactcca aagttccccc 837 Thr Ser Thr I'b t Pr o Al a O31y Ser Lys Ar g 230 235 cga 840 <210> 270 <211> 239 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 270 I'bt Trp Trp Ala Oy I'bt Ser Thr Ala I'bt Leu Ala Tyr Phe Leu On 1 5 10 Thr Val Al a Leu O31y Ph e O31y Th r Leu Leu Val Val O31n Pro Val Leu 25 Val Leu Ser Leu I'b t Phe Thr Leu Pr o Leu Ser Al a Arg Phe Asn O31y 40 Tyr Ar g Leu Ar g Ar g Thr O31u Ilie Phe Tr p Al a Thr Leu Leu Thr Val 55 Al a Val 31y Ilie 'b t Ilie Val Leu O31y Ar g Pr o Leu Pro O31y As n Pr o 70 75 Hi s Pr o Pr o Leu Asp Ar g Tr p Ilie Pr o Val Leu Leu Val (3y Val Al a 90 Page 315 seql i st cor r ec Val Met G y G y Met Trp Leu Leu Al a G u Tyr 100 105 Lys Ala Leu Ile Leu G y Leu Val Thr y Ala 115 120 Al a Val IVet Ser Lys Al a Al a Val Asp Leu Phe 130 135 Thr y Leu Ile Leu Asn Trp u y Tyr y 145 150 155 Leu Leu y Thr Ile Val n G n Tyr Ser Phe 165 170 G n Lys Ser Leu Pro Al a IVet Thr Ile Ala G u 180 185 Ser Leu y Tyr Leu Val Leu y G u Lys Phe 195 200 Su Trp lie Ala IVet y Ile Ala Leu Leu Val 210 215 Ile Ala Leu Ser Arg Thr Ser Thr IVet Pro Ala 225 230 235 <210> 271 <211> 2472 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(2449) <223> RXN00453 <400> 271 tagtggggcg tgaaaaaata gctcatttaa gaggagaagc attcaggttg gggcgatggt cctataatcg caagtggatt ted. txt Val Leu Leu Phe 125 Val Hi s 140 Leu II e Asn Ala Pro Ile G n Val 205 Met lie 220 G y Ser Lys Tyr Gy Thr G u 175 Al a Asp Ser Ar g ct a Leu ggg Gy act Thr gcc Al a gat Asp gag G u at t SIle agt Ser tct Ser gga Gy cct Pr o aat Asn gcc Al a tct Ser gtt Val aac Asn at g Met cct Pr o gtt Val act Thr aat Asn gtt Val act Thr ttt Phe ggt Gy at t SIle ttc Phe 45 ttc Phe gcg Al a ggt Gy ggt Gy gaa G u cct Pr o caa G n at g Met ggg G y ctg gcc Leu Al a 15 gac acc Asp Thr gat cag Asp n t cc ccg Ser Pro gat gca Asp Al a 80 gga gag Page 31u Page 316 aaccccgtgg cgaaat t gct gtg att tcg gca tgg Val Ile Ser Ala Trp 1 ctg acg atg cag aag Leu Thr IVet G n Lys cct tcg att gat gcc Pro Ser lie Asp Ala acg aac ccg gtg acg Thr Asn Pro Val Thr gaa gga acc acg ctt G u y Thr Thr Leu gtc gtt gat tac att Val Val Asp Tyr lle cgc ttc ggc aat cct Arg Phe y Asn Pro seql i st cor r ect ed. t xt gtt Val agc Ser gcg Al a gat Asp 150 aac Asn ggt Gy at c SIle tct Ser ggc Gy 230 aac Asn at t SIle cgc Ar g agt Ser ct c Leu 310 gcg Al a gcg Al a gt g Val ggg Gy 120 ttg Leu gag G u gcg Al a cct Pr o ggt Gy 200 at t SIle ggt Gy gt g Val t ac Tyr cca Pr o 280 gt g Val at t SIle acg Thr ttg Leu cct Pr o cct Pr o gaa G u gca Al a caa G n 170 ttc Phe ggc Gy gca Al a ct g Leu cca Pr o 250 ct g Leu gcc Al a ttt Phe gat Asp ttc Phe 330 gt g Val gcg Al a gag G u gac Asp gaa G u 155 at c SIle ggt Gy at c SIle ggc Gy gca Al a 235 gt g Val ttt Phe gat Asp gct Al a at c SIle 315 gt g Val ttt Phe ttg Leu gaa G u aaa Lys 140 t at Tyr ggt Gy gat Asp gcg Al a ttg Leu 220 at t l Ie ct c Leu at t SIle gct Al a ggc Gy 300 gga Gy gct Al a ggt Gy gaa G u acc Thr 125 acc Thr gt g Val gaa G u cca Pr o ttc Phe 205 cct Pr o gt g Val gca Al a ttg Leu gcc Al a 285 gcg Al a ttc Phe gt g Val ggt Gy gag Su 110 gct Al a at t SIle gag G u gat Asp at t SIle 190 at c SIle ttg Leu ct g Leu gt g Val tct Ser 270 gga Gy acg Thr ct c Leu ct c Leu cat Hi s 350 at g let gca Al a ggc Sy caa G n tta Leu 175 cag G n gt g Val at t SIle gcc Al a at g let 255 agg Ar g at g Met gt g Val acg Thr at t SIle 335 gcg Al a gt c Val aag Lys t ac Tyr aaa Lys 160 ggt Gy at t SIle ttg Leu acc Thr acg Thr 240 at t SIle t ac Tyr gcg Al a at t SIle gcc Al a 320 gcg Al a ttc Phe at c SI e gat Asp acc Thr 145 cac Hi s gt c Val gaa G u at t SIle gcg Al a 225 gcg Al a ggc Gy cgt Ar g gt g Val at c SIle 305 at g Met ttg Leu aag Lys gag G u gct Al a 130 tct Ser cgc Ar g cgg Ar g acc Thr ttc Phe 210 gt g Val ttt Phe ct g Leu gcg Al a ggc Gy 290 gcg Al a ggt Gy acg Thr ggc Gy cag G n 115 gcc Al a ttc Phe gat Asp gt g Val acc Thr 195 acc Thr at c SIle act Thr gcc Al a gag G u 275 aca Thr ct g Leu at t SIle ttt Phe aag Lys 355 at g Met aat Asn aac Asn gt g Val gaa G u 180 agt Ser ttt Phe ggc G y gat Asp gt g Val 260 t at Tyr gct Al a gt a Val tct Ser at c SIle 340 at c SIle acc Thr ct g Leu at t SIe at c SIe 165 gcc Al a gag G u ggt G y gt g Val ct c Leu 245 ggc G y aag Lys ggt Sy gcc Al a gcg Al a 325 ccg Pr o cct Pr o 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 gga att ggt gga Sy lie G y y aac cca acg cca aag cag acg tgg gag caa gcg ctt Asn Pro Thr Pro Lys n Thr Trp u n Ala Leu Page 317 seql i st cor r ect ed. t xt 5 370 aat Asn ccg Pr o 390 att SIle aat Asn ttt Phe gag G u gag G u 470 acc Thr aat Asn ct c Leu at g lbt act Thr 550 gag G u ttg Leu ct g Leu gcg Al a cct Pr o 360 cgt Ar g ctt Leu gca Al a gat Asp gcg Al a 440 aat Asn gaa G u at g lbt cag G n act Thr 520 gt g Val ct g Leu ct g Leu at t SIle gct Al a 600 gt g Val ccg Pr o aag Lys gt g Val aac Asn 410 act Thr gtt Val gat Asp ggc Sy gt c Val 490 at c SIle t ac Tyr cgt Ar g acc Thr gac Asp 570 ct c Leu ctt Leu gt c Val at t SIle ggt G y gca Al a 395 ct g Leu cag G n aat Asn t cc Ser gag G u 475 acc Thr agc Ser acc Thr gcg Al a act Thr 555 gca Al a ct c Leu ggc Sy tgg Tr p t cc Ser cgc Ar g 380 gt g Val cag G n cgt Ar g gcg Al a acc Thr 460 ttc Phe cag G n gt c Val gga G y cag G n 540 ggg Sy at g lbt at t SIle ttc Phe cag G n 620 ttc Phe t ca Ser gt g Val ttg Leu cag G n ccg Pr o 445 gca Al a gat Asp acc Thr aat Asn cct Pr o 525 gaa G u ttt Phe ccg Pr o ct g Leu ttg Leu 605 gag G u at g Met tgg Tr p gt c Val t ca Ser tcg Ser 430 ttc Phe ttg Leu cgg Ar g t ac Tyr gat Asp 510 gcg Al a gct Al a acg Thr gtt Val gt g Val 590 ttg Leu ggc Sy ccg Pr o gt c Val ttg Leu ct g Leu 415 gct Al a ttg Leu cag G n gag G u aat Asn 495 gat Asp gat Asp cag G n gcg Al a t ac Tyr 575 ttc Phe tct Ser ttc Phe aag ctt Lys Leu 385 ggt ctt G y Leu 400 cct tct Pro Ser gat ttg Asp Leu gt c at c Val lile cca ct g Pro Leu 465 cag gcg G n Al a 480 t cc aac Ser Asn ttc act Phe Thr aaa gag Lys G u att gag Ile G u 545 gtt cag Val G n 560 ct c gct Leu Al a cgt t cc Arg Ser gtg ggt Val G y ggt ggc 6y y 625 gt a Val ggt Sy gac Asp at g Met gt c Val 450 at t SIle gct Al a at c SIle gcg Al a acc Thr 530 gat Asp ttg Leu gt g Val ct g Leu gcg Al a 610 ttt Phe cag G n gcc Al a t cc Ser gca Al a 435 gat Asp gag G u cgt Ar g gat Asp gcg Al a 515 cct Pr o gtt Val gac Asp gtt Val ctt Leu 595 gcc Al a gt g Val aaa Lys ttg Leu acc Thr 420 gag G u acg Thr gca Al a ttt Phe gt g Val 500 cag G n gag G u acg Thr at t SIle gtt Val 580 gtt Val ttc Phe aac Asn gca Al a acc Thr 405 t cc Ser ggc Sy cat Hi s cag G n gct Al a 485 aag Lys at t SIe ttg Leu gga G y act Thr 565 ggt Sy ccg Pr o ggt Sy acc Thr 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 atc ttc ctc atc IIe Phe Leu Il e Page 318 ggc gtg acc G y Val Thr seql i st cor r ect ed. t xt 630 635 ttc ggt ttg gcc atg gac tat cag gtg ttc Phe G y Leu Al a IVet Asp Tyr G n Val Phe 650 655 gag cac tac acc cac cac aat ggc aag gga G u His Tyr Thr His His Asn G y Lys G y 665 670 acc ccg gtt gag cag tca gtg att gaa ggc Thr Pro Val u n Ser Val Ile u y 680 685 gtg gtt aca gca gcg gca ctg atc atg att Val Val Thr Ala Ala Ala Leu Ile 'e t IIe 695 700 att gat cag ccg ttg cca ttt att aag atc Ile Asp n Pro Leu Pro Phe Ile Lys Ile 710 715 gcg ggc gtg ttt ttc gat gct ttc ttc att Ala y Val Phe Phe Asp Ala Phe Phe lie 730 735 gcg tcg atg ttc ctg atg ggc aag gcc acg Al a Ser IVet Phe Leu IVet G y Lys Al a Thr 745 750 ctg gat cga att ctg cca agt ttg gac att Leu Asp Arg lie Leu Pro Ser Leu Asp lie 760 765 aag gaa tgg gag gag aag cag gct gca cgt Lys G u Trp G u G u Lys G n Ala Ala Arg 775 780 gat <210> 272 <211> 783 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 272 Val lie Ser Ala Trp Leu Leu Ile Leu Ala 1 5 10 Leu Thr IVet G n Lys G y Phe Ser Asn Ser Pro Ser Ile Asp Ala Thr Val Ser Leu Val 40 Thr Asn Pro Val Thr Al a Ala y Val Asn G u G y Thr Thr Leu Asp Asp Pro G n IVet 70 Val Val Asp Tyr Ile u Asp Asn Leu Pro 90 Arg Phe y Asn Pro Val G u Val Ser Pro 100 105 Pag 640 ctt Leu cag G n ttc Phe gcc Al a ttc Phe 720 cgc Ar g tgg Tr p gaa G u act Thr ggt Gy cag G n 690 ttc Phe ttc Phe ggt Sy at g Met acc Thr 770 cgc Ar g t cc Ser 675 ggc Sy gt g Val gcg Al a ct g Leu cct Pr o 755 gca Al a at g Met 660 aag Lys t cc Ser gcg Al a ttg Leu gt c Val 740 aag Lys ct g Leu 2083 2131 2179 2227 2275 2323 2371 2419 2469 2472 tagacttggc acctatgtca lie Phe G u Val 75 Asp I Al a I e 319 Gy I e Phe Phe Al a Sy G u Leu Asp Asp Ser Asp Sy Met Al a Thr G n Pr o Al a G u Val le O31u O31n Ntbt Thr Ser Ntbt (3 115 12 seqi i st car r ect ed. t xt y Leu Pro Olu Ou Thr Ala Ala Lys 0 125 As p Thr 145 Hi s ValI Ou le A a 225 A a O31y Ar g ValI I Ie 305 vb t Le u Ly s Tr p Le u 385 Le u Ser Le u I Ie Le u 465 A a Ph e As p ValI Thr 1 95 Thr I Ie Thr A a O31u 275 Thr Le u I Ie Ph e Ly s 355 O31n O31n A a Ser A a 435 As p (31u Le u I Ie I Ie 1 65 A a O31u O31y ValI Le u 245 O31y Ly s O31y A a A a 325 Pr o Pr o Le u A a Thr 405 Ser O31y Hi s (31n A a As p 150 As n Oy le Ser O31y 230 As n I Ie Ar g Ser Le u 310 A a A a O31y As n Pr o 390 li1e As n Ph e O31u (31u 470 ValI 135 ValI O31u O31y I Ie Le u 215 li1e As n As p vb t A a 295 li1e Ph e Le u I Ie Ar g 375 O31y Pr o I Ie O31y ValI 455 Pr o Le u O31u A a Pr a O31y 200 O31y ValI Tyr Pr a 280 ValI I Ie Thr Le u O31y 360 Ar g Le u A a As p A a 440 As n (31u Ser A a vb t A a 1 li1e A a A a Thr A a 265 Ar g ValI A a ValI O31y 345 O31y Ser ValI vb t Thr 425 O31y A a (31u O31u A a O31n 1 70 Ph e O31y A a Le u Pr a 250 Le u A a Ph e As p Ph e 330 ValI As n Ly s ValI As n 410 Thr ValI As p As p O31u 1 55 li1e Oy le Oy A a 235 ValI Ph e As p A a I Ie 315 ValI Ph e Pr a O31y A a 395 Le u O31n As n Ser Ly s 140 Tyr O31y As p A a Le u 220 li1e Le u I Ie A a O31y 300 O31y A a O31y Thr Ar g 380 ValI O31n Ar g A a Thr 460 Thr ValI O31u Pr a Ph e 205 Pr a ValI A a Le u A a 285 A a Ph e ValI O31y Pr a 365 Ser ValI Le u O31n Pr a 445 A a O31y O31n Le u 1 O31n ValI I Ie A a vb t 255 Ar g vb t ValI Thr I Ie 335 A a O31n ValI Le u Le u 415 A a Le u O31n Tyr Ly s 1 Oy le Le u Thr Thr 240 li1e Tyr A a I Ie A a 320 A a Ph e Thr Ly s O31y 400 Pr a As p ValI Pr a (31n 480 (31y (31u Ph e Asp Ar g (31u 475 Page 320 seqi i st car r ect ed. t xt Ala Ala Ar g Phe A a Thr Tyr l'bt 485 Tyr Val Thr 03 n Thr Tyr Asn Ser 490 495 As n Th r O31u O31u 545 O31n A a Ser (31y G31y 625 Le u ValI Pr o Th r ValI 705 G31y vb t Tr p G31y As p A a 515 Pr o ValI As p ValI Le u 595 A a ValI G31y Ar g Ser 675 G31y ValI A a Le u Pr o 755 A a Ly s I Ie Le u G31y Th r 565 G31y Pr o G31y Th r Th r 645 Ar g Ty r Ar g Ph e G31y 725 Pr a Tr p (31u As n Le u vb t Th r 550 G31u Le u Le u A a Pr a 630 Ph e G31u Th r ValI I Ie 71 0 A a A a Le u Ly s A a ValI Hi s 535 O31u O31n A a ValI Th r 615 G31y G31y Hi s Pr a ValI 695 As p G31y Ser As p O31u 775 I Ie 505 Pr a Le u G31y O31u Ph e 585 G31y Le u Le u A a Th r 665 O31u A a Pr a Ph e Ph e 745 li1e O31u Ser Th r A a Th r 555 A a Le u G31y Tr p Ser 635 As p Hi s Ser A a Pr a 715 As p vb t Pr a Ly s As n Pr a 525 O31u Ph e Pr a Le u Le u 605 (31u vb t O31n G31y I Ie 685 li1e I Ie Ph e Ly s Le u 765 A a As p 510 A a A a Th r ValI ValI 590 Le u G y Pr a ValI Ly s 670 O3u vb t Ly s Ph e A a 750 As p A a As p As p O31n A a Ty r 575 Ph e Ser Ph e I Ie Ph e 655 G31y G31y I Ie I Ie I Ie 735 Th r I Ie Ar g <210O> 273 <21 1> 597 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> 101). (574) <223> RXN00932 Page 321 seql i st cor r ect ed. t xt <400> 273 cccaattaat ttatgcactt cggtgaggtt actcacaaag agtagcgtgc aaagcccagc aataaggtga tgtttcaacg attaggttac ggtaggggcc atg acg cca cag aaa Met Thr Pro G n Lys ctt Leu at c l Ie cct Pr o at c SIle at c I e gca Al a t ca Ser caa G n at c SIle cgt Ar g ggc Gy gcc Al a at c SIle ggt Gy tgg Tr p ccg Pr o gt c Val 120 gt c Val gca Al a at c SIle ggt Gy gt g Val at c SIle gac Asp gaa G u cac Hi s ttc Phe gcc Al a tta Leu at c SIle tgg Tr p gt c Val 75 aag Lys aag Lys cca Pr o tct Ser ctt Leu aaa Lys cac Hi s at c SIle tct Ser aag Lys cca Pr o at c SIle at c SIle 140 tta Leu t ac Tyr ggc Gy 45 aat Asn gtt Val ggc Gy cac Hi s cga Ar g 125 ttg Leu gaa Gu agt Ser ttt Phe aat Asn at c SIle ct c Leu act Thr 110 t cc Ser ct g Leu at g Met 15 gga Gy ggc Gy aag Lys ccg Pr o gtt Val 95 ttc Phe at t SIle gcg Al a ggt Gy gt g Val ttc Phe tgg Tr p tgg Tr p gcc Al a ttt Phe tta Leu at g vbt 1 acc Thr aca Thr ct c Leu aca Thr gct Al a ggc Gy gac Asp at t SIle gga Gy 145 tgg Tr p gac Asp tgt Cys ttc Phe gca Al a gga Gy aag Lys ct g Leu 130 cca acc Thr gcc Al a ttt Phe ccg Pr o ttg Leu tgg Tr p at c SIle 115 ct g Leu cct ctg Leu gta Val gca Al a cag G n cca Pr o cgc Ar g 100 ttg Leu gtg Val t at Pro Pro Tyr cca gat gcc atc gca aac Pro Asp Ala lie Ala Asn 150 155 at g <210> 274 <211> 158 <212> PRT <213> Corynebacter i um gl <400> 274 IVet Thr Pro G n Lys Leu 1 5 Thr Trp Thr Leu Leu Ile Thr Asp Ala Val Thr Pro Leu Cys Phe Ala Ala li e act gtg gat Thr Val Asp taaacaacag cctcct t cac ut am cum His Arg Phe Ala Ala Leu Leu G u Iet y 10 I e Gy IVet lie Leu Lys Tyr Ser y Val 25 SI e Al a G y G y lie Hi s G y Phe G y Phe 40 Thr Ile Thr Val Trp Ile Asn Asn Lys Trp 55 Page 322 IO seql i st cor r ect ed. t xt Thr Phe Pro G n Gy I e Ala G y Leu I I e Val Ser Val I I e Pro Trp 70 75 Ala Ala Leu Pro Phe Ala Leu Trp Ala Asp Lys Lys y Leu Val Ala 90 GQ y Q y Trp Arg Phe Ser Asp Pro Ser G u Lys Pro His Thr Phe Phe c- 100 105 110 Asp Lys Ile Leu Ala G n Leu Val Arg His Pro Ile Arg Ser Ile Leu 115 120 125 SIl e Leu Leu Val I e I e Ala Val Val Phe Ser Ile Leu Leu Ala IVet 00 130 135 140 0 S G y Pro Pro Tyr Asp Pro Asp Al a lie Ala Asn Thr Val Asp c( 145 150 155 NO 0 <210> 275 <211> 534 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (65)..(511) <223> RXN03022 <400> 275 acgcctgtgt catccttttc attagagtgg agaaaagccc atacagaaag ttggcgcccg agca gtg atc atc acc gct ggc atc ttg gta gcg acc gcg acc gcc ctc 109 Val Ile I e Thr Al a y Ile Leu Val Ala Thr Ala Thr Ala Leu 1 5 10 cta atg atc acc gcg gtc agc gag tca acg tac atc gtc atc tcc ctc 157 Leu IVet iIe Thr Ala Val Ser u Ser Thr Tyr Ile Val lie Ser Leu 25 gcc ggc ttc tcc ctt tat ggc ctt ggc ctc gga ctc ttc gcc acc cca 205 Ala y Phe Ser Leu Tyr y Leu y Leu y Leu Phe Ala Thr Pro 40 gt c acc gat act gcg ct t gga aca ctt ccc aaa gac cgt acc ggc gct 253 Val Thr Asp Thr Ala Leu Gy Thr Leu Pro Lys Asp Arg Thr Gy Ala 55 ggt gca ggt gta ttc aag atg tcc tct tcc ctc ggc gca gca ctc ggc 301 G y Al a G y Val Phe Lys IVet Ser Ser Ser Leu G y Al a Al a Leu G y 70 atc gca atc tcc act tca gtg ttc ctc gca ctt cgc gac ggc acc tcc 349 Ile Ala lle Ser Thr Ser Val Phe Leu Ala Leu Arg Asp y Thr Ser 85 90 atc aac tcc gac gtc gca ctc gcc gga aca gtt tca ctt ggc atc aac 397 Ile Asn Ser Asp Val Ala Leu Ala Gy Thr Val Ser Leu Gy lie Asn 100 105 110 gtt gta ttc gca gca aca gcc acc atc acc gca gca gtc ctt att cca 445 Val Val Phe Ala Ala Thr Ala Thr lie Thr Ala Ala Val Leu lie Pro 115 120 125 aaa gcc gct ggc aaa gtc tca caa acc agc atc acc ctt cct gag cca 493 Lys Ala Ala G y Lys Val Ser G n Thr Ser lie Thr Leu Pro G u Pro 130 135 140 Page 323 seql i st cor r ect ed. t xt gct atc gct gta aaa atc taaaact t ca ccaggacaga taa Ala lle Ala Val Lys Ile 145 <210> 276 <211> 149 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 276 Val Ile Ile Thr Ala Gy Ile Leu Val Ala Thr Ala Th 1 5 10 Met I e Thr Ala Val Ser u Ser Thr Tyr Ile Val II 25 Sy Phe Ser Leu Tyr G y Leu y Leu y Leu Phe Al 40 4 Thr Asp Thr Ala Leu G y Thr Leu Pro Lys Asp Arg Th 55 Al a G y Val Phe Lys IVet Ser Ser Ser Leu G y Al a Al 70 75 Ala lie Ser Thr Ser Val Phe Leu Ala Leu Arg Asp G 90 Asn Ser Asp Val Ala Leu Ala y Thr Val Ser Leu G 100 105 Val Phe Ala Ala Thr Ala Thr lie Thr Ala Ala Val Le 115 120 12 Ala Ala y Lys Val Ser G n Thr Ser lie Thr Leu Pr 130 135 140 lie Ala Val Lys Ile 145 <210> 277 <211> 586 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(586) <223> RXN03151 <400> 277 ccgaacttgg agctttgctg ttggaggcag ccaaatagtc ccaatg agtatttgtt taaccatcca cctcaaggag taaaacgcac gtg ct Val Le 1 att gat gtc ctc gcc gac ccg atc gat ggc acc cca ct Ile Asp Val Leu Ala Asp Pro lie Asp y Thr Pro Le 15 gaa gat ttc tca cgg ttg gtg tct gaa tct ggg cat tc G u Asp Phe Ser Arg Leu Val Ser G u Ser G y His Se 30 Page 324 r e a r a y y u o Al a Ser Thr Gy Leu Thr SIle 110 SIle G u Leu Leu Pr o Al a Gy Ser Asn Pr o Pr o Leu Al a Val Gy SIe I e Val Lys Al a t aaa cgcact gggt t tcc cac atc 115 u Ser His Ile t gta ggc gcc 163 u Val Gy Ala c tac gat gtt 211 r Tyr Asp Val gct cgt Al a Ar g caa ggg tat gtc acc Sn Gy Tyr Val Thr seql i st cor r ect ed. t xt ctg gct ggt ggc gca gg Leu Al a G y y Al a G t ctg cgc tat y Leu Arg Tyr 0 45 5 tca ggc gat gat gca cag atg atc g Ser G y Asp Asp Ala G n Iet Ile A 60 ggc ggt cac ttc gcg ccc ttc gtg g G y G y His Phe Ala Pro Phe Val C 75 gat gtc gtt gac cag gca ggc ctt a Asp Val Val Asp G n Al a Gy Leu S tgc gaa atc ggc gcg gga acc ggc t Cys G u Ile G y Ala G y Thr G y T 105 1 tct gtt gca gga tct cgc gga att g Ser Val Ala y Ser Arg Gy Ile C 120 125 gca aag cgt ttg gca aag tgt cac c Ala Lys Arg Leu Ala Lys Cys His F 135 140 aac gca tgg gca cgc ctg ccg att g Asn Ala Trp Ala Arg Leu Pro Ile A 150 155 <210> 278 <211> 162 <212> PRT <213> Corynebacter i um gl ut am cum <400> 278 Val Leu Ser His Ile Ile Asp Val L 1 5 Pro Leu Val Gy Ala G u Asp Phe S His Ser Tyr Asp Val Al a Arg n C 40 Ala y Leu Arg Tyr Ser y Asp A G u Thr Phe Leu Ser G y G y His F 70 Thr G u His Val G n Asp Val Val A Ala n Pro Val Val Cys u Ile C 100 1 Ser His Thr Leu Asp Ser Val Ala C 115 120 Val Ser Val His Ala Ala Lys Arg L 130 135 G y Ala Val Ile Ala Asn Ala Trp A 145 150 cg l a iaa Su Igc ier ac yr gc Sy ct r o ca l a cgg Ar g gt c Val 80 gac Asp ttg Leu gac Asp gt c Val aac Asn 160 gaa G u acc Thr gca Al a t cc Ser gtt Val ggc Gy 145 t cc Ser acc Thr gag G u cag G n cat Hi s t cc Ser 130 gca Al a tcg Ser ctt Leu gtt Val gt g Val 100 ctt Leu cac Hi s at c l Ie tct Ser caa G n gtc Val gat Asp gcc Al a gcg Al a eu Ala Asp Pro lie Asp ier 25 ay sp 'he sp ay 05 ay .eu 1 a 10 Arg Leu Tyr Val Al a n I Ala Pro I 75 G n Al a 90 Al a y Ser Arg Al a Lys Arg Leu I 155 Page 325 Ser Leu I le Val Leu Sy SIle 125 Hi s SI e G u Al a Al a G u Ser Tyr 110 Sy Pr o Al a G y Thr Ser G y G y G y Asp Arg Al a Val Asp Asp Tyr Leu Ile Asp Arg Val Asp Asn 160 seql i st cor r ect ed. t xt Ser Ser <210> <211> <212> <213> <220> <221> <222> <223> 279 543 DNA Cor ynebact eri um gl ut am cum CDS (543) RXN02832 <400> 279 cgc ggg cca gtg atg Arg y Pro Val Mbet 1 5 gat tat aca aat Asp Tyr Thr Asn caa Sn 10 tca tta gta gca Ser Leu Val Al a ttt ttc Phe Phe ttt aaa gca Phe Lys Al a ctt gta gat Leu Val Asp acg tca tat tta Thr Ser Tyr Leu aaa cac aat tgt Lys His Asn Cys tta tat gtc Leu Tyr Val gac ggt gaa Asp G y G u cca tat tta att Pro Tyr Leu Ile gaa Gu aat tta cgc aat Asn Leu Arg Asn att gtt I e Val aaa tct tat gat Lys Ser Tyr Asp aac Asn 55 cga gca ttt gtt Arg Ala Phe Val aga Ar g aca atg gat aaa Thr IVet Asp Lys tta ggt tat aaa cac caa ggt ttc cct gta ggt Leu y Tyr Lys His G n Gy Phe Pro Val y 70 tat gat tca atg Tyr Asp Ser IVet caa atc cgt tgg Sn lie Arg Trp ct g Leu tca gtg tta gat Ser Val Leu Asp tta Leu 90 aaa gat aag act Lys Asp Lys Thr gaa gac Su Asp caa ctt tta G n Leu Leu aca t at gat Thr Tyr Asp 115 gaa atg gat tat G u IVet Asp Tyr caa G n 105 acg aga cgt aat Thr Arg Arg Asn at t aaa aaa I e Lys Lys 110 gat gaa acg Asp G u Thr att ggt gtc aaa lie y Val Lys aaa acg tta acg Lys Thr Leu Thr at t SIle 125 caa act G n Thr 130 ttt ttc gac tta Phe Phe Asp Leu cat at g gct gag His Met Ala G u gaa 10u 140 aag cac ggt ttc Lys Hi s G y Phe aaa Lys 145 ttc cgt gag tta Phe Arg G u Leu cca tac ttt gaa gaa atg caa aag tta tac gat Pro Tyr Phe G u G u Vet G n Lys Leu Tyr Asp 150 155 160 gac cac gcc atg Asp His Ala IVet tta Leu 165 aag ttg gcg tat Lys Leu Al a Tyr gat tta aac gag Asp Leu Asn G u tat tta Tyr Leu 175 aaa acg ttg Lys Thr Leu <210> 280 <211> 181 <212> PRT caa tta 543 G n Leu 180 Page 326 seqI i st car r ect ed. t xt <21 3> Cor ynebact er i urn gi ut ani curn Ar g Ph e Le u I Ie Le u O31n O31n Th r O31n Ly s 145 As p Ly s O> 280 G31y Pro Val Lys Al a LeL 2C Val Asp Pr c Val Lys Ser G31y Ty r LyE I Ie Ar g Tr r Leu Leu LyE 1 OC Tyr Asp I IE 115 Thr Phe PhE 130 Phe Ar g O31L Hi s Al a I'b t Thr Leu 03 r I'bt Asp Tyr Thr Asn 03 n 10 Thr Ser Tyr Leu Lys Lys 25 Ty r Leu I Ie O31u As n Leu 40 Ty r Asp Asn Ar g Al a Phe His O31n G31y Phe Pr o Val 70 Leu Ser Val Leu As p Leu 90 O3 u I'b t As p Ty r 03 n Thr 1 05 O3 y Val Lys Thr Lys Thr 120 As p Leu Phe Hi s I'b t Al a 135 Leu Pr o Ty r Phe O31u O31u 150 Leu Lys Leu Al a Ty r I Ie Ser Hi s Ar g ValI O31y Lys5 Ar g Le u O31u vb t 1 ValI cys Al a Thr As p Lys5 As n I Ie 125 Lys5 Lys5 Al a Le u As p vb t Ser Thr I Ie 110 As p Hi s Le u Ph e Tyr O31y As p vb t O31u O31u O31y Tyr Asp Leu Asn 03 u Tyr Leu 1 <210O> 281 <21 1> 1539 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (1516) <223> RXN00165 <400> 281 aaacct cccc gggcccggcg cgcgaccgt c caagat gccg gact ct caaa gt ggcgt t gt cgcagcggcc gt ggagct t t gcgt t ggat g ccaaat t at g gt g gcg t ct gct ggc at g gcg gcg I'b t Al a Al a aag gcg gt g Lys Al a Val t tc t gg at t Phe Tr p Ilie t ct tt t Ser Phe gat gag As p (1u gcc at g Al a [Vbt at c t gc aat ggg I I e Cys As n 03 y gct at t ggc acg Al a I I e 03 y Thr 30 t tg gcg gt t ct t Leu Al a Val Leu 45 Val Al a Se r Al a (31y 1 t ta acg cct gt g at t gt g ggt Leu Thr Pr o Val I Ie Val O31y 15 agc gat ct g cag cga t tg t gg Ser As p Leu O31n Ar g Leu Tr p t tc t ta acg gcg at g acg gt g Phe Leu Thr Al a N/t Thr Val Page 327 seql i st cor r ect ed. t xt aac Asn cat Hi s ttt Phe tcg Ser gt g Val at t SIle gtt Val 150 gct Al a gt g Val cgc Ar g cat Hi s ggc G y 230 ct g Leu aca Thr tcg Ser ct c Leu gag G u 310 at t SIle ttg Leu gga G y acg Thr gaa G u 120 ccg Pr o gt g Val cag G n ggc Gy cgg Ar g 200 gat Asp at c SIle ggt Sy ttt Phe tgg Tr p 280 gct Al a at t SIle cgg Ar g at g Met gag G u cgg Ar g gcg Al a ttg Leu at t SIle gcg Al a 170 aga Ar g gag G u gcg Al a gt g Val at g Met 250 at c SIle tcg Ser ttt Phe caa G n tac Tyr tt g Leu 75 cgc Ar g gt g Val tcg Ser agt Ser cag G n 155 gt g Val at t SIle gcg Al a gaa G u t cc Ser 235 agt Ser at g Met gcg Al a gag G u caa G n 315 atg ttg gtg cgg agc IVet Leu Val Arg Ser gtg act gat cgg att Val Thr Asp Arg lie act gcg ggt gga ttg Thr Al a y G y Leu 95 ggc gat atc gtc atg Gy Asp lie Val IVet 110 att att tat ggc gcc li e li e Tyr G y Al a 125 gtg gct gtg ctg att Val Ala Val Leu Ile 140 gtc tca aag ccg ttg Val Ser Lys Pro Leu 160 gca cag gct gcg gct Ala G n Ala Ala Al a 175 ttg aag ggt ttg ggc Leu Lys G y Leu G y 190 att tct ggt gag gct I e Ser G y G u Al a 205 gct cgc ttg aat ggt Ala Arg Leu Asn y 220 gcg ttg ggt att gga Ala Leu G y lie G y 240 att ggt gat ttg atc Ile G y Asp Leu Ile 255 ccg atg acc atg ctt Pro IVet Thr IVet Leu 270 gag gcg tcg gca aag G u Ala Ser Ala Lys 285 aga gtg tct gcg cat Arg Val Ser Ala His 300 ctt gcc aaa ggt ttg Leu Ala Lys G y Leu 320 cag cag ttg Sn G n Leu caa gat ccg Sn Asp Pro ttg tcg att Leu Ser Ile atg acg gt g Met Thr Val 115 gtg gtg at g Val Val Mbet 130 ggt gga ccg G y G y Pr o 145 cag aag cgt G n Lys Arg act gca act Thr Al a Thr gcg att gt c Al a I e Val 195 tat cgg aag Tyr Arg Lys 210 gt c acc gat Val Thr Asp 225 gca gga ttt Al a G y Phe acg gtt gtg Thr Val Val ggt cga aat Sy Arg Asn 275 cgt att agg Arg I e Ar g 290 gat gcg gac Asp Al a Asp 305 acg gtt att Thr Val I le gt c Val cgt Ar g gcg Al a 100 ttc Phe t ac Tyr ct g Leu tcg Ser gat Asp 180 acg Thr acg Thr gcg Al a ttg Leu gga G y 260 gt g Val gga G y aag Lys cga Ar g 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 328 seql i st cor r ect ed. t xt act gat gag cag ctc gtt gag gta tta gi Thr Asp G u G n Leu Val G u Val Leu 0 330 3; gtg att gtg gct cct cat gcg gcg gat cl Val lie Val Ala Pro His Ala Ala Asp Li 345 350 gac aat gtg cat ccc gtg gca gag gtc gi Asp Asn Val His Pro Val Ala G u Val Al 360 365 gcc t cc t gt gac gat at t cca ggt ggt ai Ala Ser Cys Asp Asp lie Pro G y G y S 375 380 ggt gga cgg ttg ctc tcg ggt ggt cag ci Gy y Arg Leu Leu Ser G y Gy n Ai 390 395 cgg gcg att gct ttt gat cca gag gtg t Arg Ala lie Ala Phe Asp Pro G u Val Li 410 4 acg gca gtg gat tct gtg acg gag caa a, Thr Ala Val Asp Ser Val Thr G u G n A 425 430 gca cac cgt gca gga aaa gtg acc att gl Ala His Arg Ala G y Lys Val Thr IIe V, 440 445 tgg agt gcg gtg gct gat caa cac gtt gi Trp Ser Ala Val Ala Asp G n His Val G 455 460 gtt atg aaa tgagtgggga gacgtcgaaa agc Val Vet Lys 470 <210> 282 <211> 472 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 282 Val Al a Ser Al a G y Mbt Al a Al a Ser PI 1 5 Pro Val lie Val G y Lys Ala Val Asp G Leu G n Arg Leu Trp Phe Trp lie Ala M 40 Thr Ala IVet Thr Val Asn Trp Ile Ala A 55 Sn G n Leu Val Ser His Asp Leu Arg M 70 G n Asp Pro Arg y Phe Ala y Lys G Leu Ser lie Ala Ser Ser Asp Thr G n A 100 105 P cag ttg cca G n Leu Pro ttt Phe gag G u t cc Ser cag G n 400 gt g Val at t SIle ttt Phe caa G n gcc Al a 370 at t SIle gtt Val caa G n cag G n gag G0u 450 cgt Ar g agt Ser 355 at t l Ie gt g Val gca Al a gat Asp caa G n 435 gca Al a act Thr 340 gt c Val gaa G u ggc G y ct g Leu ccc Pr o 420 gt g Val ccc Pr o cgg Ar g agg Ar g gtt Val gag G u gct Al a 405 aca Thr gca Al a gcg Al a 1123 1171 1219 1267 1315 1363 1411 1459 1507 1539 gca gct gct ttg cgg gag Ala Ala Ala Leu Arg G u he II e 10 u Al a et Leu rg Tyr et Leu u Ar g 90 rg Val age 329 465 Cys l Ie Al a vet Val Thr Sy Asn Sy Val Leu Thr Al a Asp Leu Ser Phe Ar g Ar g Sy Val seqI i st car r ect ed. t xt I'bt Thr Val Phe Pro Val Al a O31u Leu Al a Ser 115 120 Il e Hie Tyr 0 y Al a 125 ValI O31y 145 O31n Thr Al a Tyr ValI 225 Al a Thr O31y Ar g As p 305 Thr Le u As p Ly s Ly s 385 Ar g Le u Al a Ser vb t Pr o Ar g Thr ValI 1 95 Ly s As p Ph e ValI As n 275 Ar g As p I Ie Ar g Ser 355 li1e ValI Al a As p O31n 435 Al a Ser Le u O31y 1 65 ValI ValI ValI Al a Al a 245 Le u Al a ValI Al a O31y 325 Ar g Ar g ValI O31u Al a 405 Thr Al a Al a I Ie ValI 150 Al a ValI Ar g Hi s O31y 230 Le u Thr Ser Le u O31u 310 Thr ValI As p Al a O31y 390 Ar g Thr Al a Tr p As n 135 ValI Ar g O31n Ar g Al a 215 Al a G n O31n Ar g O31y 295 O31u As p I Ie As n Ser 375 O31y Al a Al a Hi s Ser 455 Tr p Al a O31n Le u 1 Tyr Al a Ph e G n Le u 265 Al a As p I Ie O31n Al a 345 Hi s As p Le u Al a As p 425 Al a ValI Le u I Ie Al a 1 70 Ar g O31u Al a ValI vb t 250 li1e Ser Ph e O31n Le u 330 Pr o Pr o As p Le u Ph e 410 Ser O31y Al a Ser O31n 1 ValI I Ie Al a O31u Ser 235 Ser vb t Al a O31u O31n 315 ValI Hi s ValI I Ie Ser 395 As p ValI Ly s As p ValI 140 ValI Al a Le u I Ie Al a 220 Al a I Ie Pr o O31u Ar g 300 Le u O31u Al a Al a Pr a 380 O31y Pr a Thr ValI O31n 460 Al a Ser O31n Ly s Ser 205 Ar g Le u G y vb t Al a 285 ValI Al a ValI Al a O31u 365 O31y O31y O31u O31u Thr 445 Hi s Le u Pr a Al a 1 Le u O31u As n I Ie Le u 255 vb t Al a Al a O31y O31u 335 Le u Al a Ser Ar g Le u 415 As n ValI O31u Al a Al a Leu Ar g O31u Val I'bt Lys Page 330 seqi i st car r ect ed. t xt 465 470 <210O> 283 <21 1> 1470 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1447) <223> RXN01190 <400> 283 cagggtt t tg at gagaacaa cacacaccgc t tcaagcat t gcagcggggc aaggt tgcgt t tggcgcat t ct tt t tgggg ctgcgaagaa tgatcaggcg at g t gg cag ct g t cg 11! l'bt Tr p O31n Leu Ser g aa O31u ct c Le u gt g ValI tt g Le u gcc A a gag O31u tt c Ph e gca A a ct t Le u ggc O31y 1 50 ggt O31y at c I Ie tt g Le u aaa Ly s tt t Ph e ag g Ar g cat Hi s at g vb t c ag O31n ac c Thr 120 ct g Le u t ct Ser c ag O31n G31y ccg Pr o ct c Le u gt g ValI gt g ValI tt g Le u cgg Ar g gga G31y ct g Le u ccg Pr o ag g Ar g agt Ser 1 70 cgt Ar g gcg A a cga Ar g agt Ser tt t Ph e aat As n ac c Thr ggt G31y at c I Ie at t I Ie 140 gt c ValI at c I Ie gcc A a at t I Ie tt a Le u tt t Ph e 45 g aa (31u ct g Le u gca A a gt t ValI agt Ser 125 t cg Ser ac c Thr at g vb t gt g ValI ggt G31y gt g ValI t ct Ser t cc Ser g at As p g at As p t cg Ser 110 g ac As p gga G31y c ag O31n at g vb t aag Ly s 1 90 tt g Le u 15 gt c ValI aat As n cat Hi s ccg Pr o gcg A a 95 gct A a t gg Tr p gt g ValI c ag O31n ggg O31y 1 75 act Thr at c I Ie ggg G y cgt Ar g gcg A a cgc Ar g g at As p gcg A a ct g Le u gt t ValI gag O3u 1 ct g Le u tt t Ph e 1 gt t ValI ct t Le u tt c Ph e ct c Le u aat As n t ct Ser ac g Thr gt c ValI gca A a 145 aag Ly s cgc Ar g g aa O31u g at As p gt c ValI ggt O31y cgc Ar g tt g Le u t cg Ser gga O31y ggg O31y 130 ct g Le u tt g Le u gt g ValI aag Ly s cat Hi s gct A a t cg Ser gt a ValI gt g ValI ac g Thr tt t Ph e 115 tt g Le u gct A a gcg A a at c I Ie gcg A a 1 gcg A a tt t Ph e cgc Ar g gag O31u cct Pr o cgt Ar g 1 00 ct t Le u tt g Le u ag c Ser gag O31u aag Ly s 1 t cg Se r Page 331 seql i st cor r ect ed. t xt gca tca gcg aga gcg Ala Ser Ala Arg Ala 200 lcg gtt gat I a Val Asp 205 act gca gtt gct tcg ggc aaa gtc Thr Ala Val Ala Ser y Lys Val gct Al a ttg Leu 230 ttg Leu ttg Leu gag G u gaa Gu cca Pr o 310 gct Al a cat Hi s ct g Leu acg Thr agg Ar g 390 agc Ser cag G n ttt Phe at t l Ie gct Al a gca Al a agc Ser gt g Val 280 agc Ser agc Ser gac Asp gac Asp gac Asp 360 agc Ser ttg Leu gt g Val cga Ar g aac Asn gag G u tgg Tr p gt g Val 250 tcg Ser aat Asn aca Thr ctt Leu ttc Phe 330 gt g Val at t SIle tta Leu gca Al a t ca Ser 410 ggc Gy tt g Leu cgg Ar g 235 ggt Gy at t SIle tt c Phe at c SIle cca Pr o 315 gaa G u cca Pr o gag G u tcg Ser gac Asp 395 gt g Val aaa Lys t cc Ser 220 gt c Val gt g Val aat Asn tta Leu aat Asn 300 cac Hi s ggt Gy at t SIle gt g Val ggt Gy 380 gcg Al a acg Thr acc Thr at t SIle acc Thr gcg Al a gcc Al a aac Asn 285 gac Asp ggc Gy acc Thr gat Asp gac Asp 365 ggg Gy gaa Gu gag Gu acc Thr gcg Al a acg Thr gt g Val t ca Ser 270 ct c Leu ggc Gy gac Asp ttg Leu ccg Pr o 350 gga Gy cag G n gt a Val gt g Val at t SIle 430 gt g Val ggg Gy t at Tyr 255 gct Al a gac Asp gaa Gu aat Asn cgg Ar g 335 cag G n ctt Leu cgt Ar g ct g Leu tct Ser 415 gt g Val aat Asn gag G u 240 ttg Leu at t l Ie gaa G u ttc Phe at c SIle 320 t ca Ser gt a Val gat Asp cag G n gt g Val 400 at c SIle gt g Val ttg Leu 225 ttg Leu t ca Ser gcg Al a tct Ser ct c Leu 305 ttg Leu aat Asn at t SIle gcg Al a cga Ar g 385 ct g Leu gcg Al a agt Ser 210 gct gcg Al a Al a ggg cct G y Pro gag ccg G u Pro cac ggt Hi s G y 275 cag gca G n Al a 290 gt c at c Val I e gct aca Al a Thr att tcc Ile Ser cgc gct Arg Ala 355 ccg gtg Pro Val 370 gtg gct Val Ala atg gat IVet Asp cag ggg G n y tct tcg Ser Ser 435 gtg ttg Val Leu ggc cag G y G n 245 att cgc li e Arg 260 gca gcg Al a Al a cag tac G n Tyr gtg ccc Val Pro cct cat Pro His 325 at g aat IVet Asn 340 tct ggt Ser G y cgc gat Arg Asp ttg gcc Leu Al a cca acc Pro Thr 405 att aag I e Lys 420 ccc gcg Pro Al a 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1457 1470 gcg gat cgg gtg att tca cat gtc Ala Asp Arg Val lie Ser His Val 445 t aat t t gat g 440 gcatcatcga cac <210> 284 Page 332 seqI i st car r ect ed. t xt <21 1> 449 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 284 N/t Tr p (31n Leu Ser GuAla LeuVal Pro IleAla Ile(3y Leu Ilie ValI Le u Ph e Le u As n Ser Thr ValI A a 145 Ly s Ar g O31u A a Le u 225 Le u Ser A a Ser Le u 305 Le u Hi s A a Ser ValI ValI Thr Ph e 115 Le u A a A a I Ie A a 1 95 G31y A a Pr a Pr a G31y 275 A a I Ie Th r ValI ValI A a ValI G31y li1e G31y ValI Ly s Ser 1 65 A a O31n ValI Le u O31n 245 Ar g A a Tyr Pr a Hi s 325 Le u ValI Le u A a 70 O31u Ph e A a Le u G31y 150 Gy le A a A a Le u 230 Le u Le u O31u O31u Pr a 310 A a Thr Le u As n As p ValI G31y A a ValI 135 li1e A a G31y Ser G31y 215 Le u I Ie Le u Ar g Ser 295 A a A a Ly s Ph e 40 Ar g Hi s vb t O31n Thr 120 Le u Ser O31n O31y A a 200 li1e A a A a Ser ValI 280 Ser Ser As p 10 Asp Leu Val Val Al a Val Al a Leu Ser Ar g 90 le O31y 1 Tyr Leu Val Pr o Lys Ar g A a Ser 1 70 O31u Ar g 1 Ar g Ala O31y (1u O31y Tr p I e Val 250 Asn Ser 265 A a As n O3 u Thr Thr Leu I I e Phe 330 Ar g Le u As n Ly s 75 Ser Thr Le u I Ie Ser 1 55 As p Tr p A a Le u Ar g 235 O31y I Ie Ph e I Ie Pr a 315 Le u Ph e O31u Le u A a ValI Ser 125 Ser Thr vb t ValI As p 205 li1e Th r A a A a As n 285 As p (31y ValI As n Hi s Pr a A a A a Tr p ValI O31n O31y 1 Thr A a ValI O31y Tyr 255 A a As p O31u As n O31y Ar g A a Ar g As p A a Le u ValI O31u 1 Le u Ph e ValI As n O31u 240 Le u I Ie O31u Ph e I Ie 320 O31u O31y Thr Leu Ar g Ser 335 Page 333 As n I Ie Al a Ar g 385 Le u Al a Ser ValI Ser Al a 355 ValI Al a As p G31y Ser 435 As n G31y As p Al a Thr 405 Ly s Al a G31u Thr G31y 375 Al a Al a Le u Tyr seql i st car r ect ed. t xt Asp Asn Val Pro I Ie Asp 345 As p I Ie IIe G31u Val As p 360 365 Ser Asn Leu Ser G3 y G3 y 380 Leu Hi s Al a As p Al a G3 u 395 Val As p Ser Val Thr O31u 410 Ar g Al a G31y Lys Thr Thr 425 Asn Leu Ala Asp Arg Val 440 445 O31n Le u Ar g Le u Ser 415 ValI Ser <210O> 285 <21 1> 1368 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1345) <223> RXN01102 <400> 285 at tct atggt t gt tgggaga gat gact taat t tggaat ca cat tgagcaa ct tcccagca t gt ggaaaag cccaggt t tc cgggct t taa cacgcgct ga gt g gct gt c ct c gt g 11! Val Al a Val Leu Val gcg Al a ccg Pr o ac c Thr ccc Pr o gca Al a cca Pr o gca Al a gcg Al a ggc G31y gcg Al a at g vb t cca Pr o gcg Al a ct c Le u tt c Ph e cgg Ar g ggt G31y ct g Le u t t c Ph e aac As n at g vb t aaa Ly s gt g ValI 75 gt a ValI ggc G31y aac As n gca Al a at t I Ie cca Pr a gt g ValI t gg Tr p gga G31y ac a Thr t ac Tyr at c I Ie gca Al a t ca Ser 15 t ca Ser gt g ValI ac c Thr ggg G31y ct t Le u 95 ct c Le u ag c Ser at c I Ie cca Pr a t ac Tyr 80 cga Ar g 1 ct t Le u gct Al a act Thr gt g ValI 65 at c I Ie gga G31y ct t Le u gt c ValI c ag O31n 50 at g vb t tt c Ph e at t I Ie ccc Pr a gcc Al a 35 at t I Ie gct Al a ag c Ser ggg G31y gt c ValI 20 ggt G31y tt c Ph e tt c Ph e gt c ValI tt c Ph e 1 00 gt a ValI gcc Al a act Thr gcc Al a gag O31u ggt G31y gcg ct c acc gt c gca gaa t ct gcg t tg gt g gct gaa ct c gt t ccc gt a Al a Leu Thr Val Al a O31u Ser Al a Leu Val Al a O31u Leu Val Pro Val Page 334 seql i st cor r ect ed. t xt cgc Ar g t cc Ser ggc Gy 150 gcg Al a caa G n gt g Val gca Al a tta Leu 230 t ca Ser gt g Val gt c Val ttg Leu aac Asn 310 t cc Ser at c SIle ggt Gy acc Thr ttg Leu 120 at g Met aac Asn at g Met cag G n gtt Val 200 tct Ser gct Al a gt g Val ggt Gy tta Leu 280 at a SIle gcg Al a gcc Al a agc Ser ttt Phe 360 gcc Al a aaa Lys ttc Phe gt c Val ct g Leu 170 agc Ser t cc Ser ttc Phe tta Leu cgc Ar g 250 acg Thr acc Thr gca Al a ctt Leu gcc Al a 330 ct c Leu t cc Ser cga Ar g gct Al a ct g Leu t at Tyr 155 cgt Ar g gaa G u tt g Leu ctt Leu gcg Al a 235 tac Tyr at g Met gt c Val gt g Val t ca Ser 315 at c l Ie ctt Leu gga Gy at c SIle tct Ser cct Pr o 140 gtt Val at t SIle cag G n gct Al a cca Pr o 220 ggt Gy ct g Leu at t SIle aca Thr at g Met 300 at g Met tgg Tr p ggc Gy gcc Al a at t SIle gga Gy 125 gcc Al a tta Leu ccg Pr o gag G u gtt Val 205 gct Al a at t SIle t cc Ser cct Pr o at c SIle 285 ttt Phe ttt Phe aat Asn at a SIle gt g Val 365 ggg Gy 110 atg ttg ggc IVet Leu G y ggg ttg gcg G y Leu Al a ggt gcc gtt G y Al a Val 160 cag gtt aag G n Val Lys 175 cgt tct gt t Arg Ser Val 190 acc agt ttg Thr Ser Leu gca gt c at t Ala Val lile att tta tcc I e Leu Ser 240 ggc gtt atc S y Val lile 255 gct cag at c Ala G n Il e 270 ttc caa ggc Phe G n G y ggt ggt gct G y G y Al a ttc cgg ctt Phe Arg Leu 320 atc gcc ttt I le Al a Phe 335 gtt gcc gca Val Al a Al a 350 gtg att ttg Val Ile Leu cgg cac cgc Arg His Ar g gt a Val tta Leu 145 at c SIle gca Al a t cc Ser t ca Ser gag G u 225 at t SIle gct Al a at t SIle tgg Tr p ttt Phe 305 cct Pr o gat Asp tcg Ser ttt Phe ttt Phe 130 ggt Sy gca Al a gcg Al a acc Thr at g Met 210 tta Leu acc Thr gac Asp ggg G y t cc Ser 290 ggc Sy cgc Ar g tcg Ser ctt Leu gga Sy 370 at t SIle gac Asp ct a Leu gca Al a tgg Tr p 195 act Thr gat Asp ggt G y cgc Ar g ttc Phe 275 gt g Val at g Met act Thr gga Sy gct Al a 355 at c SIle ggc G y caa G n gtt Val aag Lys 180 aag Lys ttt Phe cca Pr o ggt G y cgc Ar g 260 tta Leu tgg Tr p gt g Val aga Ar g aca Thr 340 t ac Tyr gtt Val 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 att act gaa tac aac Ile Thr u Tyr Asn Page 335 IND seql i st cor r ect ed. t xt 375 380 385 Saac acc cgc gcg cgt ttg cgc cag gtg cca gtc gct cgg cgt gca gtg 1315 Asn Thr Arg Ala Arg Leu Arg G n Val Pro Val Ala Arg Arg Ala Val 390 395 400 405 d) S caa ggg ctg cgc aac agg cgc aaa gat cgc taaaacgctt ttcgacgcca 1365 SQn y Leu Arg Asn Arg Arg Lys Asp Arg 410 415 ccc 1368 0 <210> 286 00 <211> 415 0 <212> PRT S<213> Cor ynebact eri um gl ut ai cum <400> 286 SVal Ala Val Leu Val Al a Val Ala Ala Ala Phe y Ser Trp Ser Leu 1 5 10 (N Leu Leu Pro Val Val Pro Leu Ala Val Leu Asn Asn G y G y Ser Ser 25 Al a Val Al a G y Al a Thr Thr G y Ile Phe Met Al a Ala Thr Val Ile 40 Thr n lie Phe Thr Pro Ala Ala Leu Arg Lys lie y Tyr Thr Pro 55 Val IVet Al a Phe Al a Ala Phe IVet Leu G y Val Pro Ala I e y Tyr 70 75 Ile Phe Ser Val G u Pro Ile Pro Val Leu Val Val Ser Ala Leu Arg 90 Sy Ile y Phe y Ala Leu Thr Val Ala u Ser Ala Leu Val Ala 100 105 110 G u Leu Val Pro Val Arg Phe Leu y Lys Ala Ser y IVet Leu y 115 120 125 Val Phe Ile G y Leu Ser G n IVet Leu Phe Leu Pro Al a G y Leu Al a 130 135 140 Leu y Asp G n Phe G y Tyr Asn Val Val Tyr Val Leu y Ala Val 145 150 155 160 Ile Ala Leu Val Ala Al a Val Iet Cys Leu Arg Ile Pro G n Val Lys 165 170 175 Ala Ala Ala Lys n n Pro n Val Ser u n u Arg Ser Val 180 185 190 Ser Thr Trp Lys Leu Val Leu Val Pro Ser Leu Ala Val Thr Ser Leu 195 200 205 Ser IVet Thr Phe y Al a Val Ser Ser Phe Leu Pro Ala Ala Val lie 210 215 220 Su Leu Asp Pro y Leu y Ala Ala Leu Ala y lie lie Leu Ser 225 230 235 240 I e Thr G y G y Ser Ser bet Val Phe Arg Tyr Leu Ser G y Val Ile 245 250 255 Ala Asp Arg Arg y Val Pro y Thr Thr IVet lie Pro Ala n lie Page 336 SIle Tr p Phe 305 Pr o Asp Ser Phe SIle 385 Al a Phe 275 Val Met Thr Gy Al a 355 I le G u Ar g Gy Leu G n Val 325 Sy Ser Leu Asn Val 405 Val SIle 295 G u G u Gy Al a Thr 375 Thr Sy seql i st cor r ect ed. t xt 265 Leu Ile Thr Val Thr Ile 280 285 I e G y Al a Val Mbt Phe 300 Ala Leu Leu Ser IVet Phe 315 Ala Ser Ala lie Trp Asn 330 Ser Phe Leu Leu Gy Ile 345 Phe G y Ser G y Al a Val 360 365 Al a Asp Arg lie lie G y 380 Arg Ala Arg Leu Arg G n 395 Leu Arg Asn Arg Arg Lys 410 G n Gy Ar g Al a 335 Al a SIle Hi s Pr o Ar g 415 Gy Al a Leu 320 Phe Al a Leu Ar g Val 400 <210> 287 <211> 348 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(325) <223> RXN00788 <400> 287 cgcatccctc tagttttcca tc ttgatccagc actgcttgca gt aac Asn ggt Gy ggc Gy gtt Val gag Gu gga Gy acc Thr ttg Leu cgt Ar g cag G n ttc Phe acc Thr acg Thr aaa Lys gcc Al a aac Asn act Thr t cc Ser gcg Al a caa Sn ;acctcaat gaacggcgct aactccggtt cat t gcgcaa gaggcctc attagttggc atg gcc tcc tcc atc IVet Ala Ser Ser Ile 1 ctt gga aat gct gtt gct gcc tgg ctt gct Leu y Asn Ala Val Ala Ala Trp Leu Ala 15 tcc ctt gga ctc aca tca gcc gga tta gtt Ser Leu y Leu Thr Ser Ala y Leu Val ctc gga cta gtg ttg gcc atc gtg gct gtg Leu y Leu Val Leu Ala lle Val Ala Val 45 caa ggc acc caa gcg acc atc agc gtt gtg Sn y Thr n Ala Thr Ile Ser Val Val taaataat tt ctctcttcta att Page 337 seql i st cor r ect ed. t xt <210> 288 <211> <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 288 IVet Al a Ser Ser I e Asn I e y Val Phe Asn Leu y Asn Ala Val 1 5 10 Ala Ala Trp Leu Ala G y Ala Thr I e Thr Thr Ser Leu y Leu Thr 25 Ser Ala y Leu Val G y y Leu bet Thr Ser Leu y Leu Val Leu 40 Ala lie Val Ala Val Val Leu Arg Arg Lys Ala G n y Thr G n Ala 55 Thr lie Ser Val Val G u His G n Pro Ala G n 70 <210> 289 <211> 1764 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1741) <223> RXN02119 <400> 289 ttcggtccgc tctggcaaaa atggctggct gccacctcgg cgcagcagct taagggctgg gcttaaattg cttgtcgacg cctagtgcca caatggagac gt g Val gat Asp gt c Val gaa G u gt g Val gca Al a at g Met aat Asn aat Asn ggc Gy cca Pr o t ac Tyr t ac Tyr gac Asp ctt Leu acc Thr ggt Gy gct Al a cca Pr o gct Al a gcc Al a gca Al a gt a Val aaa Lys gga Gy cag G n 75 cgt Ar g gaa G u ggc G y gct Al a t cc Ser act Thr gaa G u cga Ar g gcg Al a ggc G y gcc Al a aca Thr 45 at c SIle cac Hi s acc Thr ttt Phe t at Tyr ggc G y ttt Phe gcg Al a acc Thr ggc Gy ggt Gy 110 ggg G y 15 gat Asp ct a Leu ctt Leu cgc Ar g tgc Cys 95 gcg Al a cac Hi s gt c Val aaa Lys tcg Ser acg Thr caa G n gat Asp at g Met 1 cgc Ar g gt g Val at t SIle cca Pr o tct Ser gct Al a cca Pr o aca Thr ggc G y ct g Leu gcc Al a gga G y gca Al a gac Asp tta Leu gtt Val gcg Al a gat Asp gag G u aca Thr aac Asn 115 ttt Phe gtc Val ggc G y gct Al a acg Thr act Thr 100 gct Al a acc gaa aca Thr G u Thr ttg gcc gat gca tac gcc gag gcg ctg gat cgg tgg atg gcc agt ggc Page 338 Leu Al a Asp 120 Ala Tyr Al a G u Al 12 seql i st cor r ect ed. t xt a Leu Asp Arg Trp IVet Al a Ser G y 5 gca Al a ttt Phe 150 gca Al a gt g Val caa G n agc Ser ct c Leu 230 t cc Ser t at Tyr acg Thr gca Al a gaa G u 310 tta Leu gt c Val gca Al a gt a Val gat Asp ctt Leu cgg Ar g ct c Leu gag G u 200 gat Asp ct g Leu ctt Leu gaa G u cgt Ar g 280 gac Asp cag G n gaa G u aag Lys ttc Phe 360 gct Al a gat Asp acc Thr ggg G y 170 gag G u ttt Phe gag G u caa G n gaa G u 250 gcg Al a tgg Tr p gac Asp caa G n gaa G u 330 tcg Ser caa G n gat Asp gaa G u tcg Ser 155 ct g Leu ccc Pr o gtt Val ttt Phe aat Asn 235 cgc Ar g gaa G u tct Ser aaa Lys aaa Lys 315 gag G u cgg Ar g ggc G y cgc Ar g cgc Ar g 140 acg Thr gcg Al a acc Thr cag G n ctt Leu 220 t cc Ser gca Al a aag Lys agt Ser ctt Leu 300 gt c Val cca Pr o t ca Ser gat Asp at t SIle 380 ccc Pr o at g Met tta Leu gat Asp 190 ctt Leu agg Ar g cat Hi s ct a Leu aag Lys 270 ggt G y aag Lys cag G n aaa Lys tct Ser 350 acc Thr at c I le at c SIle gaa G u ct g Leu 175 ttg Leu cgc Ar g tgt Cys gtt Val cgc Ar g 255 gac Asp gt c Val aaa Lys at g Met gaa G u 335 gtt Val ttg Leu aca Thr gt g Val gga G y 160 ttg Leu gat Asp ggg G y gt g Val t at Tyr 240 cag G n ctt Leu cgc Ar g gcc Al a gaa G u 320 tgg Tr p gtt Val gga gay gga G y ct c Leu 145 ctt Leu t ca Ser ct c Leu gga G y acc Thr 225 ggc G y cac Hi s gt g Val aat Asn gct Al a 305 agc Ser aaa Lys t cc Ser cca Pr o ccc Pr o 385 130 gct Al a t ca Ser cgt Ar g gac Asp gt c Val 210 act Thr ggt G y gcc Al a gca Al a gct Al a 290 gcg Al a cgc Ar g ct g Leu acg Thr gt a Val 370 aac Asn gat Asp ggc G y ttt Phe ggt G y 195 gt a Val gt g Val gga G y cgt Ar g cgt Ar g 275 at t l Ie gaa G u at c SIle cag G n ttg Leu 355 t cc Ser ggt G y ttg Leu ggg G y gac Asp 180 ctt Leu ct g Leu ct g Leu t at Tyr gac Asp 260 gct Al a aaa Lys t cc Ser gct Al a ttc Phe 340 aat Asn at c SIle gct Al a ggc G y cag G n 165 at t SIe gag G u gt c Val gaa G u gat Asp 245 caa G n cga Ar g cgt Ar g agt Ser cgg Ar g 325 agc Ser gat Asp caa G n ggt G y 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 aaa tcc aca ttg ctg cgc gga cta ttg gga aac caa gaa ccc acc agc Page 339 s Lys Ser Thr Leu Leu Arg G y Leu 390 395 eql i st cor r ect ed. t xt Leu G y Asn G n G u Pro Thr Ser ggt Sy gca Al a aag Lys aaa Lys tct Ser 470 ggc G y gag Su ttg Leu aat Asn gcc Al a gcg Al a gtt Val 440 ggg G y ggc G y aac Asn at c SIle ct g Leu 520 cgt Ar g at g Met 410 ctt Leu gac Asp aat Asn cgc Ar g ctt Leu 490 caa G n acg Thr cat Hi s acg Thr cca Pr o ccg Pr o aat Asn 460 cgc Ar g ctt Leu gag G u gat Asp gag G u 540 agc Ser cag G n at c SIle 445 cat Hi s gcc Al a gat Asp caa G n cgt Ar g 525 gct Al a gt g Val ttg Leu 430 agt Ser gt g Val gga G y gag G u gcg Al a 510 cgc Ar g ggc G y gcg Al a 415 cca Pr o gag G u gaa G u ctt Leu ccc Pr o 495 ttg Leu at g Met gaa G u 400 at c I le ct g Leu gt g Val cgg Ar g gcg Al a 480 acc Thr gcc Al a ttg Leu gtt Val gga G y at t SIle cgc Ar g gac Asp 465 ct g Leu aac Asn tcg Ser gac Asp agg Ar g 545 gaa G u t ct Ser aca Thr 450 gt c Val ct a Leu cac Hi s t at Tyr gct Al a 530 gag G u at c SIle gcg Al a 435 ct g Leu gaa G u cag G n ctt Leu gat Asp 515 gt g Val ct a Leu gat Asp 420 ttt Phe ct c Leu aag Lys gt g Val gac Asp 500 ggt a y cag G n 1363 1411 1459 1507 1555 1603 1651 1699 1741 1764 taaccgtttc cgtattgatg cca <210> 290 <211> 547 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 290 Met Thr G u Thr Leu Val Val Asn y 1 5 Arg Thr Leu Phe Asn Asp Val Asn Leu 25 Val y Val Val y Val Asn y Ala 40 Ile Leu Ala y Val G u Lys Pro Leu 55 Pro Ala Asp Ala Phe Val y Tyr Leu 70 Ser y u Thr Ile Ala Val Tyr lle Al a Al a Thr Thr Al a Iet Asp Asp Thr 100 105 Leu Al a G y 10 Thr Val Al a G y Lys Ser Al a G y Thr Pro G n G u Al a Ar g Ar g 90 Ala G u Ala Page 340 Sy Al a Thr I le Hi s Thr Phe Tyr Sy Phe Al a Thr Sy Gy 110 Sy Asp Leu Leu Ar g Cys Al a seqi i st car r ect ed. t xt Pro Asp Asn Ala Ala Leu Ala 115 Tr p Le u 145 Le u Ser Le u O31y Thr 225 (31y Hi s ValI As n Al a 305 Ser Ly s Ser Pr o Pr o 385 O31n O31y I Ie Ar g Al a As p O31y Ph e O31y 1 95 ValI ValI G31y Ar g Ar g 275 li1e Ou le O31n Le u 355 Ser O31y Pr o I Ie Al a 435 Le u O31y O31y O31n 1 65 li1e O31u ValI O31u As p 245 O31n Ar g Ar g Ser Ar g 325 Ser As p O31n O31y Ser 405 O31n O31u Al a Al a Ph e 150 Al a ValI O31n Ser Le u 230 Ser Tyr Thr Al a O31u 310 Le u ValI Al a ValI Ly s 390 O31y Al a Ly s Ly s Al a 135 O31u Al a Le u Le u Hi s 215 As p Tyr O31u O31n Pr o 295 Ly s O31u O31y Ser As n 375 Ser Thr Ar g Hi s Ph e 455 As p 120 As p Le u Ar g Le u O31u 200 As p Le u Le u O31u Ar g 280 As p O31n O31u Ly s Ph e 360 Al a Thr Al a Al a ValI 440 O31y Al aTyr Al a 0 u Al a Leu Asp 125 Le u Pr a ValI As p 1 As n Ar g Hi s G u Ph e 265 O3u As n Al a ValI Al a 345 Thr O3y Le u Thr Le u 425 Pr a Le u O31u Ser 1 Le u Pr a ValI Ph e As n 235 Ar g O31u Ser Ly s Ly s 315 O31u Ar g O31y Ar g Ar g 395 O31y As p Le u As p Ar g 140 Thr Al a Thr O31n Le u 220 Ser Al a Ly s Ser Le u 300 ValI Pr a Ser As p I Ie 380 O31y Thr Pr a Pr a As n 460 I Ie Le u Al a As n O31y 205 Ser Hi s ValI Ly s Hi s 285 Ar g Ar g Ar g Ser Ph e 365 O31y Le u Ser O31n I Ie 445 Hi s I Ie O31u Le u 1 Le u Ar g cys ValI Ar g 255 As p ValI Ly s vb t O31u 335 ValI Le u Thr O31y Al a 415 Pr a O31u O31u Asp Val Ou Lys Leu Ser Pro O1y Ou Arg Thr Arg Ala Oly Leu Ala Page 341 seqi i st car r ec 465 470 475 Leu Leu G31n Val Ar g G31y Val Asn Val Leu Val 485 490 Asn Hi s Leu Asp Leu a3 u A a Il e a3 u a3 n Leu 500 505 Ser Tyr Asp a3 y Val Leu Leu Leu Val Thr Hi s 515 520 Asp Ala Val a31n Thr Asn Ar g Ar g Tr p Hi s Val 530 535 Ar g a31u Leu 545 <210> 291 <211> 1638 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221> OIDS <222> (1615) <223> RXN01605 <400> 291 attgaagtca gcgccgagga agccgcgagg gaatcagacg cat tgaaat t ccgaagaact t cagcgat tc t at tgccagc ted. t xt 480 Leu Asp a31u Pro Thr 495 03 u 03 n A a Leu A a 510 As p Ar g Ar g I'bt Leu 525 a3 u A a a3 y a3 u Val1 540 acgggcacct act act tcgg gt g acc agc gat t ca 11! Val Thr Ser Asp Ser ccc Pr o at t I Ie at g vb t tt c Ph e ac g Thr gcc A a ggt a31y a31y cca Pr o t cc Ser ac g Thr ac c Thr ag c Ser ggc a31y c aa a31n gcg A a 120 ac c Thr ct g Le u tt c Ph e ggc a31y a31y gt c ValI ct t Le u ac a Thr gt c ValI ggc a31y a31y gac As p gt c ValI 75 acc Thr gcc A a ggc a31y aac As n aac As n gt c ValI ggc a31y ggt a31y ct g Leu g ac As p ag c Ser gcg A a c ag a31n cgc Ar g 45 at g vb t t cc Ser cgc Ar g ggc a31y c aa a31n 125 gt a ValI gt g ValI at t I Ie aac As n gcc A a g ac As p gcc A a 110 ac g Thr tt c Ph e 15 gt c ValI gt g ValI c aa a31n aac As n ggc a31y 95 ag c Ser ct c Le u aac As n aac As n g at As p gcc A a g ac As p at c I Ie c ag a31n gcc A a 1 aac As n act Thr aac As n gcc A a ggt a31y gca A a ct c Le u g ac As p ag c Ser gt t ValI at g vb t g aa a31u gca A a agt Ser g ac As p ggt a31y 130 aac As n gt g ValI ct c Le u ggt a31y gt t ValI gcc A a ac c Thr 115 ct a Le u ggc a31y gag a31u gt c ValI gcc A a c ag a31n aat As n 1 00 ggc a31y t cc Ser ctg tct gcg ggc acc gcc caa cta ggc caa ggc gca acc cag gtt tca Page 342 seql i st cor r ect ed. t xt Leu Ser Al a G y Thr Al a G n Leu G y G n y Al a Thr G n Val Ser 135 gat Asp 150 gtt Val acc Thr gt g Val acc Thr gct Al a 230 ggc G y agc Ser ttc Phe caa G n ttc Phe 310 ct g Leu cga Ar g caa G n cca Pr o gt a Val 390 gt g Val gac Asp gcc Al a t cc Ser 200 aaa Lys ggc G y caa G n ct c Leu gac Asp 280 gga G y gct Al a cca Pr o acc Thr acc Thr 360 cac Hi s at c SIle caa G n aac Asn 170 gaa G u gt g Val gga G y agc Ser ct g Leu 250 ct c Leu gca Al a acc Thr ggc G y agt Ser 330 gca Al a at g Met gct Al a at t SIle 140 gt c Val cag G n tct Ser gca Al a agc Ser 220 ttg Leu gac Asp ct c Leu acc Thr ccg Pr o 300 ttc Phe cgc Ar g t ac Tyr gca Al a ct g Leu 380 cat Hi s gac Asp ttg Leu gat Asp t cc Ser 205 caa G n gcc Al a ggc G y acc Thr acc Thr 285 ct c Leu at g Met gca Al a ctt Leu gt a Val 365 tgg Tr p at g Met caa G n at c SIle ccc Pr o 190 cag G n ct c Leu gca Al a acc Thr gac Asp 270 at c SIle ttc Phe gga G y ct c Leu cca Pr o 350 ct g Leu at g Met ttc Phe gt a Val acc Thr 175 t cc Ser caa G n agc Ser ggt G y gt c Val 255 ggc G y gca Al a ggt G y gca Al a gac Asp 335 t ca Ser t ac Tyr gcg Al a aac Asn gca Al a 160 ct g Leu agc Ser ct a Leu at c SIle t cc Ser 240 cag G n gca Al a acc Thr at t SIle acc Thr 320 t cc Ser aca Thr ttc Phe at g Met aac Asn 400 145 cca Pr o cgc Ar g acc Thr gca Al a ggt G y 225 gaa G u ctt Leu agc Ser cca Pr o ggt G y 305 gtt Val cgc Ar g gt c Val ct g Leu gt c Val 385 gt g Val ct g Leu gac Asp t ac Tyr gcc Al a 210 gca Al a cag G n gat Asp caa G n gtt Val 290 ct c Leu gcc Al a at g let tta Leu ct c Leu 370 gcc Al a gca Al a acc Thr ggc G y cgc Ar g 195 ggc cgc Ar g ct a Leu gaa G u gt a Val 275 gaa G u gca Al a tgg Tr p gga G y ggc G y 355 gac Asp at c l Ie gga G y gcc Al a gca Al a 180 t cc Ser ct g Leu acc Thr gtt Val ggc G y 260 cca Pr o aca Thr cca Pr o at g Met ggc Gy 340 ctt Leu ct c Leu t ca Ser ccc Pr o t at Tyr 165 gcc Al a ggc G y caa G n ct c Leu gtt Val 245 t cc Ser acc Thr gaa G u ttc Phe at c SIe 325 ttc Phe ggc G y aat Asn tgg Tr p t cc Ser 405 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gca ggc cgt gtg ctg tcc atc gtg atg atg tcc ttc cag cta gtc tcc Page 343 Al a t cc Ser ttc Phe at g Met at c SIle 470 ct g Leu gaa Gu Ar g ggc G y acc Thr 440 ttc Phe gt a Val aat Asn aag Lys seql i st cor r ect ed. t xt Leu Ser lie Val Met Met Ser Phe G n 410 415 tac cca cca gaa acc cag cca gca ttc Tyr Pro Pro G u Thr G n Pro Ala Phe 430 gac ccg atc acc tac gca gtc aac ctc Asp Pro lie Thr Tyr Ala Val Asn Leu 445 450 gaa acc cca tcc aac gac cca cgc ttc G u Thr Pro Ser Asn Asp Pro Arg Phe 460 465 ctc ttc atc tgg gca ctg atg ctc gcc Leu Phe lie Trp Ala Leu IVet Leu Ala 475 480 aca aac aag gtt ctt cgc atg aag gac Thr Asn Lys Val Leu Arg IVet Lys Asp 490 495 taaaagcttt tcccgcccgg ttc Val 420 cac Hi s cgc Ar g caa G n t cc Ser cac Hi s 500 Ser tgg Tr p caa G n gca Al a acc Thr 485 cca Pr o 1411 1459 1507 1555 1603 1638 <210> 292 <211> 505 <212> PRT <213> Corynebact <400> 292 Val Thr Ser Asp 1 Asn Ser Asn y Thr Val Val G u Asn IVet Leu Val Al a G u y Al a Sy Ala Val n Ala Ser Ala Asn 100 Leu Asp Thr G y 115 Asp G y Leu Ser 130 Ala Thr G n Val 145 Pro Leu Thr Ala er i um gl ut ari cum Ser Pro Ala Pro Ala 5 Phe Thr Sy Thr Leu G u Leu Ser Ser Tyr 165 Al a Asp Ser Leu Asp Al a Ser Ser 135 G y Pr o Ser Thr 40 Thr Ser Sy G n Al a 120 Al a Val Asp bet 25 G u Leu Asp Al a Ser 105 Al a G y Gy lie Val Sy Sy Asp Val Thr Al a Sy Al a Leu 155 Ser Al a G n Ar g Met Ser Ar g Sy G n 125 Leu Asp Leu Val Val I le Asn Al a Asp Al a 110 Thr G y G n lie Phe Val Val G n Asn Sy Ser Leu G n Val Thr 175 Arg Asp Oy Ala Ala Thr I e Ala Ser u Leu Ser Asp Pro Ser Ser Page 344 seqi i st car r ect ed. t xt 1 85 A a ValI Se r 1 G3 n G3 n Leu Thr A a G31y 225 O31u Le u Ser Pr o G31y 305 ValI Ar g ValI Le u ValI 385 ValI Ph e A a As n Ar g 465 Le u Ly s Ar g 1 95 G31y Ar g Le u O31u ValI 275 O31u A a Tr p G31y G31y 355 As p I Ie G31y Le u Ph e 435 ValI I Ie I Ie Tyr G31y (31n Le u ValI 245 Ser Thr O31u Ph e I Ie 325 Ph e G31y As n Tr p Ser 405 Ser Tr p O31n A a Thr 485 ValI Thr A a 230 G31y Ser Ph e O31n Ph e 310 Le u Ar g O31n Pr o ValI 390 A a Ser Ph e vb t I Ie 470 Le u As p Le u 215 As p A a O31u A a A a 295 vb t Hi s G31y A a A a 375 Ph e G31y G31y Hi s I Ie 455 Tr p A a As p Thr A a A a 265 G31y As p ValI I Ie Le u 345 li1e Pr o Ser ValI Le u 425 Tyr As n Le u Ar g ValI 505 (31y Ser Le u 250 Le u A a Thr G31y Ser 330 A a vb t A a I Ie Le u 410 Tyr As p O31u Le u Thr 490 Ser O31n 235 Ar g Ly s As p Thr Le u 315 Ar g Ser Tr p O31y Thr 395 Ser Pr o Pr a Thr Ph e 475 As n A a Se r 205 Ser 03 n 220 Leu A a As p (1y Leu Thr Thr Thr 285 Pro Leu 300 Phe l'b t Ar g Ala Tyr Leu A a Val 365 Leu Trp 380 Hi s l'b t I e Val Pro O31u I I e Thr 445 Pr o Ser 460 li1e Tr p Lys Val Le u A a Thr As p 270 li1e Ph e O31y Le u Pr a 350 Le u vb t Ph e vb t Thr 430 Tyr As n A a Le u Ser O31y ValI 255 O31y A a O31y A a As p 335 Ser Tyr A a As n vb t 415 O31n A a As p Le u Ar g 495 I Ie Ser 240 O31n A a Thr I Ie Thr 320 Ser Thr Ph e vb t As n 400 Ser Pr a ValI Pr a vb t 480 vb t Pr o 03 u Leu Lys <210O> 293 <21 1> 664 <212> DNA <21 3> Cor ynebact er i urn gi ut anm cumn Page 345 seqi i st car r ect ed. t xt <220> <221 OIDS <222> (664) <223> RXN01091 <400> 293 t cgt cggaaa aact ggcgat act at tgat a acgggcat gg ggt ct at gct ggact at cgc ct t t t gacac gagt at cgca gt tcacct tc gat aaagcac gt c ValI ct g Le u t gg Tr p g aa G u ct a Le u c aa O3n gca A a c ag O3n at c I Ie ggc O3y 1 50 aac As n ct t Le u at c I Ie cat Hi s at t I Ie t ac Tyr O31y gcg A a t cg Ser aac As n 120 gcg A a gcg A a gcc A a aag Ly s g ac As p at g vb t c ag O31n gt t ValI tt g Le u gag O31u aag Ly s aaa Ly s gag O31u gga G31y cgc Ar g 1 70 ac c Thr gaa O31u gcg A a acc Thr gcc A a ggc G31y 75 gca A a at g vb t t cc Ser cgc Ar g ct g Le u 155 gt t ValI gag O31u ac c Thr t gc cys tt t Ph e g ac As p g at As p ggc G31y gt t ValI gcc A a gcg A a 140 cct Pr o ggg G31y g aa O31u gcc A a ct g Le u gcc A a 45 gcc A a tt g Le u cat Hi s tt g Le u g ac As p 125 ggc O31y gcc A a tt t Ph e g at As p cgc Ar g ag c Ser at c I Ie ct g Le u tt c Ph e cgc Ar g 110 c ag O31n aaa Ly s ct g Le u g at As p ct g Le u 15 g at As p at c I Ie gcg A a tt t Ph e gct A a 95 cat Hi s c ag O31n gcg A a at g vb t t gg Tr p 1 75 gcg A a ccg Pr o gcg A a c ag G n c ag O3n 80 tt g Le u ccg Pr o gt g ValI c aa O3n cgc Ar g 1 60 ccc Pr o at g vb t 1 ac g Thr gt c ValI ccc Pr a g aa G u ac c Thr gt t ValI c ac Hi s g aa O3u aag Ly s 145 gcg A a g ac As p gt c ccg aac Val Pr o As n c ag O31n ag c Ser c ac Hi s g ac As p gt g ValI g ac As p gt g ValI g at As p 130 ggc O31y ac g Thr at t I Ie c ag O31n t gc cys at t I Ie ccc Pr a c ac Hi s gt g ValI 1 00 ggc O31y g aa O31u tt g Le u ct g Le u c ag O31n 1 ac a Thr Ar g ccg Pr a gag O31u gag (31u gcc A a aag Ly s gcg A a gt c ValI g at As p c aa O31n 1 gt a ValI <210O> 294 <211> 188 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 294 l'b t ValI Pr o As n Th r ValI L eu I Ie Hi s As p O31u Th r Ala As p L eu Ala Page 346 seql i st cor r ect ed. t xt Arg Leu u His IVet Al a Cys Leu Thr Val Pr o G u Thr Val Hi s G u Lys 145 Al a Asp SIle Gy Thr Tr p Phe Val Phe 115 Tr p Val Lys Gy Pr o G u G u Al a Lys Al a Val Asp G n 165 Val Asp Gy Ar g Mbet SIle Ser Lys 135 Val Asn Gy I e 40 Tyr Gy Al a Ser Asn 120 Al a Al a Al a Lys I e G u G u G u Ar g Asp 105 Al a Pr o Leu Al a Val 185 Thr Al a Gy Al a vbt Ser Ar g Leu 155 Val G u Phe Asp Asp Gy Val Al a Al a 140 Pr o Gy G u Al a Al a Leu Hi s Leu Asp 125 Gy Al a Phe Asp SIe Al a Phe Al a Hi s G n Al a Met Tr p 175 <210> 295 <211> 357 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(334) <223> RXS02979 <400> 295 ctaggtcaag gaact t cact cggctagtcc tta gcaccgtaag gcacgaaagt taccgaaagg act igactcaa atgtgt t cag acaaacactg ggttccc atg acc gcc cca aac 115 IVet Thr Ala Pro Asn 1 gat gag ttc tct tgc cca tcc 163 Asp G u Phe Ser Cys Pro Ser 15 aat gga ttg gat ggc gtc gac 211 Asn Gy Leu Asp G y Val Asp aga atc ctt gtt gat cac gac 259 Arg lie Leu Val Asp His Asp gtc gct gca gtc gca gag gtt 307 Val Al a Al a Val Al a G u Val Page 347 act Thr tgt Cys aat Asn ccc Pr o aag Lys t cc Ser gag G u aag Lys aca Thr at t SIle aag Lys tct Ser act Thr gaa G u tt c Phe at c SI e ctt Leu aac Asn t cc Ser aag Lys cgc Ar g aaa Lys t cc Ser 45 gat Asp tct Ser ttg Leu gga G y ct a Leu seqI i st car r ect ed. t xt ggc t ac acc gca aag cca t ca gca at c t aaaact ct c agt tagacca Gy Tyr Thr Ala Lys Pro Ser Ala Ilie t ta <210O> 296 <21 1> 78 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ani cumn <400> 296 I'bt Thr Al a Pro Asn Thr Leu Lys O31n Thr Thr Leu Arg Ser A 1 5 10 Phe Ser Cys Pr o Ser Cys Val Ser Lys Ilie GuAsn Lys Leu A 25 Leu Asp a3 y ValI As p As n Al a G3 u Val Lys Phe Ser Ser G3 y A 40 Leu Val Asp Hi s Asp Pr o Ser Lys Val Ser I Ie Lys Asp Leu V 55 Al a Val Al a G31u Val G31y Ty r Thr Al a Lys Pr o Ser Al a Ilie 70 <210> 297 <211> 357 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (334) <223> RXS02987 <400> 297 gt tgt t tgat ccaggt caag gaat taaccc ggaaaggacc gt atct t taa ag acaggaacat gacgataaaa gatgaaagga cctggttacg atg acc gcc c sp a3 u Sn ay rg le aI Al a gt gcaagc cc gcc ac g Thr t gt Cys aat As n cca Pr o ggt G31y aag aag Ly s gcc Al a gag (31u aag Ly s ac c Thr ac c Thr at c I Ie aag Ly s t cc Ser aag Ly s acc Thr gaa (31u t t c Ph e gt a ValI ccg Pr o tt g Le u aac As n t cc Ser cgt Ar g t cg Ser t ct Ser ct g Le u gga G31y ct g Le u at c I Ie I'b t Thr Ala Pro Ala 1 gat gag t tc acc tgt ccg agc Asp G31u Phe Thr Cys Pr o Ser 15 aat ggt t tg gac ggc gt g gag As n G31y Leu As p G31y Val G31u cgc atc cCt g at c acc cac gac Ar g Ilie Leu Ilie Thr Hi s Asp gt c acc gcg gt a gcc gag gt c Val Thr Al a Val Al a G31u Val t gacgcact c ccgaccccac Page 348 seqI i st car r ect ed. t xt <210O> 298 <21 1> 78 <21 2> PRT <21 3> COr ynebact er i urn gi ut arri cumn <400> 298 I'bt Thr Al a Pro Al a Thr Leu Lys Asn Thr Thr 1 5 10 Phe Thr Cys Pr o Ser Cys Val Ala Lys lie Ou 25 Leu Asp G3 y Val 1G3 u As n Al a G3 u ValI Lys Phe 40 Leu I Ie Thr Hi s As p Pr o O31n Lys Val Ser Val 55 Al a Val Al a O31u Val G31y Ty r Th r Al a Lys Pr o 70 <210O> 299 <21 1> 492 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (469) <223> RXS03095 <400> 299 aacgcctcaa ttagtgccag accttgccga ccgcagacca t ccct agcca caacaacggc agt t gt gcaa t gat ct gcgt Leu Ar g Ser Asp O31u Asn Lys Leu Asn 03 y Ser Ser G31y Ar g I Ie Arg Asp Leu Val Thr Ser Al a Il e aact tcacca t t tcaaacca at g aat gca gat aag I'bt Asn Al a Asp Lys aaa g aa O31u gca Al a gt c ValI at g vb t aaa c ag (31n t gc Cys tt c Ph e cga Ar g aaa Lys5 cga Ar g ac c Thr g aa (31u at g vb t ct c Le u agt Ser ccc Pr o gt g ValI g aa O31u ac c Thr ccg Pr o gcg Al a g aa O31u gca Al a ac c Thr gca Al a gcg Al a g at As p g ac As p ct t Le u 45 gt t ValI cgt Ar g t ca Ser g ac As p ag c Ser gcc Al a t cc Ser t cc Ser c aa O31n c ag O31n (31y 110 c aa O31n 15 act Thr gt a ValI c aa O31n g aa O31u ct a Le u 95 c ag (31n t ac Tyr cga Ar g aac As n c ac Hi s ggt O31y gt c ValI act Thr 1 gt c ValI gt t ValI c ac Hi s ct c Le u c aa O31n t cc Ser ccc Pr o g aa O31u cgc Ar g ct c Le u gcc Al a cga Ar g g ac As p cca Pr o ct t Le u at c I Ie gcg Al a cgg Ar g gt t ValI gct Al a c ac Hi s 115 gcc Al a at c I Ie g ac As p ct g Le u tt c Ph e at t I Ie 1 00 c ac Hi s Page 349 INO seqI i st car r ect ed. t xt cga gaa cga gaa caa tca tgaccaccca cagtcaccaa gaa 492 SAr g 1u Ar g (1u 1n Se r 120 S<210> 300 <211> 123 ri<21 2> PFT <21 3> Cor ynebact er i urn gi ut anm curn <400> 300 Ibt Asn Al a Asp Lys Lys [Vbt Cys G31y [Vbt Asn Pro Asp Ser O31n Tyr 1 5 10 00 SVal O31u Leu Al a Val O31u Val Phe G31y Leu Le u Al a Asp Al a Th r Ar g 25 .C Val Ar g I Ie Ilie Leu Ala Leu Arg Asn Ser G31y O31u Leu Ser Val Asn 40 (i Hi s Leu Al a Asp Ilie Val Asp Lys Ser Pr o Ala Ala Val Ser On His 55 Leu Ala Ar g Leu Ar g I'b t Al a Ar g Ilie Val Ser Thr Arg O31n O31u O31y 70 75 O31n Ar g Val Phe Tyr Lys Leu Thr Asn O31u Hi s Al a Ser O31n Leu Val 90 Ser Asp Al a Ilie Phe O31n Al a O31u Hi s Thr Ilie Al a As p G31y O31n Th r 100 105 110 Pr o Pr o Hi s Hi s Hi s Ar g O31u Ar g O31u O31n Ser 115 120 <210> 301 <211> <21 2> PFT <21 3> Cor ynebact er i urn gi ut anm curn <400> 301 Ala Pro Ala Leu Oy Pro Thr Leu Ser Oy 1 5 <210> 302 <211> <212> PFT <213> Cor ynebact er i urn gi ut anm curn <220> <221> VARIANT <222> 2-4, 7, 8 <223> Xaa any anm no aci d <400> 302 O31y Xaa Xaa Xaa O31y Pr o Xaa Xaa O31y O31y 1 5 <210> 303 <211> <212> DNA <213> Cor ynebact er i urn gi ut anm curn <400> 303 Page 350 NO seql i st cor r ect ed. t xt Sctccaggatt gctccgaagg <210> 304 <211> <212> DNA J <213> Cor ynebact eri um gl ut ari cum <400> 304 cacagtggtt gaccactggc <210> 305 0 <211> 18 00 <212> DNA S<213> Artificial Sequence C(1 <220> sC) <223> Description of Artificial Sequence: prirrer 0 <400> 305 rCl ggaaacagta tgaccatg 18 <210> 306 <211> 17 <212> DNA <213> Arti fi ci al Sequence <220> <223> Descri ption of Artifi cial Sequence: pr i rrer <400> 306 gtaaaacgac ggccagt 17 Page 351