MX350668B - Gen sintetico para expresion de schistosoma mansoni-14 en pichia pastoris, procedimientos de produccion de schistosoma mansoni-14 y su uso como vacuna y medio de diagnostico. - Google Patents

Gen sintetico para expresion de schistosoma mansoni-14 en pichia pastoris, procedimientos de produccion de schistosoma mansoni-14 y su uso como vacuna y medio de diagnostico.

Info

Publication number
MX350668B
MX350668B MX2016003662A MX2016003662A MX350668B MX 350668 B MX350668 B MX 350668B MX 2016003662 A MX2016003662 A MX 2016003662A MX 2016003662 A MX2016003662 A MX 2016003662A MX 350668 B MX350668 B MX 350668B
Authority
MX
Mexico
Prior art keywords
producing
pichia pastoris
purifying
synthetic gene
protein
Prior art date
Application number
MX2016003662A
Other languages
English (en)
Inventor
Miriam Tendler
Celso Raul Romero Ramos
Andrew J G Simpson
Original Assignee
Fundacao Oswaldo Cruz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao Oswaldo Cruz filed Critical Fundacao Oswaldo Cruz
Publication of MX350668B publication Critical patent/MX350668B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C07K14/43559—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from trematodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0003—Invertebrate antigens
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a el campo de la producción de proteínas recombinantes usando un gen sintético asociado con la expresión de proteínas superiores en Pichia pastoris; más específicamente, esta invención describe la producción de la proteína recombinante Sm14 de Schistosoma mansoni; se creó un gen sintético para promover la expresión de Sm14 superior, y con este gen se obtuvo y manipuló genéticamente una cepa de Pichia pastoris para producir una vacuna de forma efectiva; también se han mejorado los procedimientos de producción y purificación de esa proteína a partir de células de P. pastoris; se puede programar la producción industrial de tales procedimientos.
MX2016003662A 2010-09-17 2011-09-13 Gen sintetico para expresion de schistosoma mansoni-14 en pichia pastoris, procedimientos de produccion de schistosoma mansoni-14 y su uso como vacuna y medio de diagnostico. MX350668B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1005855A BRPI1005855B1 (pt) 2010-09-17 2010-09-17 processos de produção e purificação de proteina sml 4 recombinante em pichia pastoris
PCT/BR2011/000320 WO2012034197A1 (pt) 2010-09-17 2011-09-13 Gene sintético para expressão de sm-14 em pichia pastoris, processos de produção e purificação de sm-14 e seu uso vacinai e de diagnóstico

Publications (1)

Publication Number Publication Date
MX350668B true MX350668B (es) 2017-09-12

Family

ID=45830887

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2013003005A MX339728B (es) 2010-09-17 2011-09-13 Gen sintético para expresión de schistosoma mansoni-14 en pichia pastoris, procedimientos de producción de schistosoma mansoni-14 y su uso como vacuna y medio de diagnóstico.
MX2016003662A MX350668B (es) 2010-09-17 2011-09-13 Gen sintetico para expresion de schistosoma mansoni-14 en pichia pastoris, procedimientos de produccion de schistosoma mansoni-14 y su uso como vacuna y medio de diagnostico.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2013003005A MX339728B (es) 2010-09-17 2011-09-13 Gen sintético para expresión de schistosoma mansoni-14 en pichia pastoris, procedimientos de producción de schistosoma mansoni-14 y su uso como vacuna y medio de diagnóstico.

Country Status (9)

Country Link
US (2) US9193772B2 (es)
EP (1) EP2617829B1 (es)
AU (1) AU2011301714B2 (es)
BR (1) BRPI1005855B1 (es)
MX (2) MX339728B (es)
NZ (1) NZ609370A (es)
PE (1) PE20140424A1 (es)
WO (1) WO2012034197A1 (es)
ZA (1) ZA201302741B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102017001309A2 (pt) * 2017-01-23 2018-08-14 Fundação Oswaldo Cruz vacina recombinante para helmintos em pichia pastoris, e, processos de produção e purificação de proteína como vacina para helmintos
CN112195192A (zh) * 2020-09-30 2021-01-08 贵州大学 一种利用酵母表达***有效表达NaD1蛋白的方法
BR102021012953A2 (pt) 2021-06-29 2023-01-10 Fabp Biotech Desenvolvimento Em Biotecnologia Ltda. Composição de vacina veterinária contra helmintos, método para tratamento e prevenção de infecção causada por helmintos e uso

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0303266B8 (pt) * 2003-01-31 2021-05-25 Fundacao Oswaldo Cruz proteína recombinante sm14, composição imunogênica, e,kit de diagnóstico.

Also Published As

Publication number Publication date
ZA201302741B (en) 2018-12-19
EP2617829A4 (en) 2014-05-07
AU2011301714B2 (en) 2016-07-14
MX2013003005A (es) 2013-10-03
BRPI1005855B1 (pt) 2020-04-22
EP2617829B1 (en) 2019-08-28
WO2012034197A8 (pt) 2012-06-07
BRPI1005855A2 (pt) 2013-01-15
PE20140424A1 (es) 2014-03-28
NZ609370A (en) 2015-03-27
WO2012034197A1 (pt) 2012-03-22
US20160052963A1 (en) 2016-02-25
AU2011301714A8 (en) 2013-06-06
US20130237475A1 (en) 2013-09-12
MX339728B (es) 2016-06-06
US9193772B2 (en) 2015-11-24
EP2617829A1 (en) 2013-07-24
AU2011301714A1 (en) 2013-05-09
US9475838B2 (en) 2016-10-25

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