MX345399B - Metodo de cultivo de celulas animales. - Google Patents

Metodo de cultivo de celulas animales.

Info

Publication number
MX345399B
MX345399B MX2013007650A MX2013007650A MX345399B MX 345399 B MX345399 B MX 345399B MX 2013007650 A MX2013007650 A MX 2013007650A MX 2013007650 A MX2013007650 A MX 2013007650A MX 345399 B MX345399 B MX 345399B
Authority
MX
Mexico
Prior art keywords
culturing
animal cell
cell culturing
cells
culturing method
Prior art date
Application number
MX2013007650A
Other languages
English (en)
Other versions
MX2013007650A (es
Inventor
Shohei Kishishita
Tomoko Okui
Yasuharu Shinoda
Shinya Takuma
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of MX2013007650A publication Critical patent/MX2013007650A/es
Publication of MX345399B publication Critical patent/MX345399B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/303—Liver or Pancreas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para reducir un nivel de picos ácidos de un anticuerpo deseado mientras el anticuerpo se prepara al cultivar una célula animal que produce el anticuerpo, en donde la célula animal es una célula que tiene tal carácter que la productividad del anticuerpo deseado por célula no se eleva o decae a una temperatura más baja que una temperatura de cultivo norma y en donde el cultivo se realiza a la temperatura de cultivo normal por un cierto periodo y después el cultivo se continúa a una temperatura de cultivo reducida a 25-35°C.
MX2013007650A 2010-12-28 2011-12-28 Metodo de cultivo de celulas animales. MX345399B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010291636 2010-12-28
PCT/JP2011/080478 WO2012091124A1 (ja) 2010-12-28 2011-12-28 動物細胞の培養方法

Publications (2)

Publication Number Publication Date
MX2013007650A MX2013007650A (es) 2013-08-01
MX345399B true MX345399B (es) 2017-01-30

Family

ID=46383211

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013007650A MX345399B (es) 2010-12-28 2011-12-28 Metodo de cultivo de celulas animales.

Country Status (17)

Country Link
US (1) US20130295613A1 (es)
EP (1) EP2660328A4 (es)
JP (1) JP6001456B2 (es)
KR (1) KR101903208B1 (es)
CN (2) CN107629124A (es)
AU (1) AU2011350456B2 (es)
BR (1) BR112013018751B1 (es)
CA (1) CA2822947A1 (es)
HK (1) HK1248242A1 (es)
IL (1) IL227185B (es)
MX (1) MX345399B (es)
MY (1) MY163081A (es)
NZ (1) NZ611862A (es)
RU (1) RU2615448C2 (es)
SG (1) SG191371A1 (es)
WO (1) WO2012091124A1 (es)
ZA (1) ZA201304332B (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US10144923B2 (en) 2012-12-10 2018-12-04 Seikagaku Corporation Recombinant Factor C and method for producing the same, and method for measuring endotoxin
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
CN103255238A (zh) * 2013-05-15 2013-08-21 江苏泰康生物医药有限公司 一种控制哺乳动物细胞乳酸生成的方法
CN105431454A (zh) * 2013-07-06 2016-03-23 卡迪拉保健有限公司 用于生产单克隆抗体的改进方法
CN106170298B (zh) 2013-10-16 2024-01-09 前瞻疗法公司 用于提高抗体稳定性的缓冲液制剂
EP3247718B1 (en) 2015-01-21 2021-09-01 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
TW202340452A (zh) 2015-08-04 2023-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
KR101789509B1 (ko) * 2015-11-05 2017-10-26 주식회사 프로젠 재조합 인간 갑상선 자극 호르몬을 포함하는 조성물 및 상기 재조합 인간 갑상선 자극 호르몬의 생산 방법
JP2019500878A (ja) * 2016-01-06 2019-01-17 オンコバイオロジクス，インコーポレイティド モノクローナル抗体組成物中の高分子量種、酸性荷電種、及び断片の低減
JP7084308B2 (ja) 2016-02-03 2022-06-14 アウトルック セラピューティクス，インコーポレイティド 抗体安定性を増大させるための緩衝液製剤
SG11201908891YA (en) * 2017-03-31 2019-10-30 Boehringer Ingelheim Int Perfusion medium
CN111500662A (zh) * 2018-06-25 2020-08-07 深圳市菲鹏生物制药股份有限公司 调节cho-k1表达***所分泌抗体的酸性峰含量的方法
WO2023063611A1 (ko) * 2021-10-12 2023-04-20 프레스티지바이오로직스 주식회사 항체 집단의 제조 방법

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RO105652B1 (ro) * 1988-09-28 1992-11-30 Lilly Co Eli Procedeu de reducere a heterogenitatii anticorpilor monoclonali
JP4306813B2 (ja) 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
TW426733B (en) * 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
EP1497444B1 (en) * 2002-03-27 2015-11-04 Immunex Corporation Methods for increasing polypeptide production
ATE460670T1 (de) * 2002-07-08 2010-03-15 Ge Healthcare Uk Ltd Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen
CN111925445A (zh) * 2004-07-09 2020-11-13 中外制药株式会社 抗-磷脂酰肌醇蛋白聚糖3抗体
KR101495549B1 (ko) * 2006-07-13 2015-02-25 와이어쓰 엘엘씨 당단백질의 생산
JP5586235B2 (ja) 2007-03-02 2014-09-10 ワイス・エルエルシー ポリペプチドの産生のための細胞培養における銅およびグルタミン酸塩の使用
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
WO2009012394A1 (en) * 2007-07-17 2009-01-22 Medarex, Inc. Monoclonal antibodies against glypican-3
BRPI0818674B1 (pt) * 2007-10-15 2023-01-17 Chugai Seiyaku Kabushiki Kaisha Métodos para produção de um anticorpo inteiro ou de um fragmento do mesmo e para preparação de fármacos, bem como vetor recombinante
EP2236604B1 (en) 2007-12-05 2016-07-06 Chugai Seiyaku Kabushiki Kaisha Anti-nr10 antibody and use thereof
PL2702164T3 (pl) * 2011-04-29 2016-06-30 Biocon Res Limited Sposób obniżania heterogeniczności przeciwciał i sposób ich wytwarzania

Also Published As

Publication number Publication date
JP6001456B2 (ja) 2016-10-05
BR112013018751B1 (pt) 2021-01-05
JPWO2012091124A1 (ja) 2014-06-05
AU2011350456B2 (en) 2016-05-26
CA2822947A1 (en) 2012-07-05
SG191371A1 (en) 2013-08-30
IL227185B (en) 2018-05-31
NZ611862A (en) 2015-05-29
MX2013007650A (es) 2013-08-01
MY163081A (en) 2017-08-15
RU2615448C2 (ru) 2017-04-04
EP2660328A4 (en) 2016-07-13
HK1248242A1 (zh) 2018-10-12
EP2660328A1 (en) 2013-11-06
KR101903208B1 (ko) 2018-10-01
CN107629124A (zh) 2018-01-26
KR20130131415A (ko) 2013-12-03
WO2012091124A1 (ja) 2012-07-05
RU2013135255A (ru) 2015-02-10
AU2011350456A1 (en) 2013-07-04
BR112013018751A2 (pt) 2016-08-09
US20130295613A1 (en) 2013-11-07
ZA201304332B (en) 2014-08-27
CN103282509A (zh) 2013-09-04

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