MX2023004594A - Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas. - Google Patents

Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas.

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Publication number
MX2023004594A
MX2023004594A MX2023004594A MX2023004594A MX2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A
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cells
corneal endothelial
human corneal
time
sup
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MX2023004594A
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Inventor
Shigeru Kinoshita
Munetoyo TODA
Chie Sotozono
Morio Ueno
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Kyoto Prefectural Public Univ Corp
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Publication of MX2023004594A publication Critical patent/MX2023004594A/es

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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    • C12N1/04—Preserving or maintaining viable microorganisms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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Abstract

Las células endoteliales de la córnea humana y/o las células precursoras endoteliales de la córnea humana se conservan con una alta tasa de supervivencia de estas células manteniéndose, y se suprime la tasa de aparición de células contaminadas en el cultivo posterior a la conservación. Un método de almacenamiento de células endoteliales de la córnea humana y/o células precursoras endoteliales de la córnea humana se caracteriza porque las células endoteliales de la córnea humana y/o las células precursoras endoteliales de la córnea humana que han sido cultivadas utilizando un medio de cultivo que contiene un inhibidor de ROCK, y en el que el contenido de factor de crecimiento epidérmico (EGF) es menor que una concentración que causará una transformación se recolectan en un tiempo en que se cumple cualquiera de o una pluralidad de las condiciones (a) ~ (d) que se indican a continuación, y se colocan en un estado de suspensión y luego de conservado. (a) Durante un período inmediatamente después de que una morfología de las células endoteliales de la córnea humana y/o de las células precursoras endoteliales de la córnea humana haya cambiado de una forma fusiforme a una forma poligonal o una forma elíptica cuya proporción eje mayor-eje menor es cercana a 1, hasta inmediatamente antes de que los límites entre las células se vuelvan indistintos. (b) Durante un período desde que un nivel de expresión de CD44 se vuelve igual o inferior a la mitad del valor máximo observado después del subcultivo más reciente hasta que este nivel de expresión alcanza una meseta. (c) Cuando la densidad celular de las células endoteliales de la córnea humana y/o de las células precursoras endoteliales de la córnea humana no sea inferior a 900 células/mm2 ni superior a 2500 células/mm2. (d) Cuando el número de días de cultivo desde el subcultivo más reciente no sea inferior a 4 días ni superior a 14 días.
MX2023004594A 2020-10-22 2021-10-19 Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas. MX2023004594A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020177634 2020-10-22
PCT/JP2021/038609 WO2022085680A1 (ja) 2020-10-22 2021-10-19 ヒト角膜内皮細胞及び／又はヒト角膜内皮前駆細胞の保存方法

Publications (1)

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MX2023004594A true MX2023004594A (es) 2023-05-08

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MX2023004594A MX2023004594A (es) 2020-10-22 2021-10-19 Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas.

Country Status (10)

Country Link
US (1) US20230380417A1 (es)
EP (1) EP4234682A1 (es)
JP (2) JP7361431B2 (es)
KR (1) KR20230091107A (es)
CN (1) CN116583593A (es)
AU (1) AU2021366805A1 (es)
CA (1) CA3196110A1 (es)
IL (1) IL302168A (es)
MX (1) MX2023004594A (es)
WO (1) WO2022085680A1 (es)

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EP4234682A1 (en) 2023-08-30
JPWO2022085680A1 (es) 2022-04-28
CA3196110A1 (en) 2022-04-28
WO2022085680A1 (ja) 2022-04-28
IL302168A (en) 2023-06-01
JP7361431B2 (ja) 2023-10-16
CN116583593A (zh) 2023-08-11
AU2021366805A1 (en) 2023-06-22
KR20230091107A (ko) 2023-06-22
US20230380417A1 (en) 2023-11-30
JP2023139164A (ja) 2023-10-03

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