MX2019011619A - Metodo y kit para la purificacion de proteinas. - Google Patents
Metodo y kit para la purificacion de proteinas.Info
- Publication number
- MX2019011619A MX2019011619A MX2019011619A MX2019011619A MX2019011619A MX 2019011619 A MX2019011619 A MX 2019011619A MX 2019011619 A MX2019011619 A MX 2019011619A MX 2019011619 A MX2019011619 A MX 2019011619A MX 2019011619 A MX2019011619 A MX 2019011619A
- Authority
- MX
- Mexico
- Prior art keywords
- kit
- protein
- purification method
- protein purification
- interest
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000001742 protein purification Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000700605 Viruses Species 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 230000003750 conditioning effect Effects 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/04—Inactivation or attenuation; Producing viral sub-units
- C12N7/06—Inactivation or attenuation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención provee un método y un kit para la inactivación y la captura/purificación de virus combinadas, de un material que contiene una proteína de interés; el método comprende los pasos de: poner en contacto un material que comprende una proteína de interés con partículas o medios cargados negativamente, acondicionar las partículas o los medios de modo que el pH sea ácido, inactivando de esta manera de dicho virus, y eluir dicha proteína de interés.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE2017/5211A BE1025090B1 (fr) | 2017-03-30 | 2017-03-30 | Procede et kit de purification de proteines |
PCT/EP2018/058365 WO2018178375A1 (en) | 2017-03-30 | 2018-03-30 | Protein purification method and kit |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2019011619A true MX2019011619A (es) | 2019-11-18 |
Family
ID=59269719
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2019011619A MX2019011619A (es) | 2017-03-30 | 2018-03-30 | Metodo y kit para la purificacion de proteinas. |
Country Status (9)
Country | Link |
---|---|
US (1) | US20210095261A1 (es) |
EP (1) | EP3601315A1 (es) |
KR (1) | KR20190135495A (es) |
BE (1) | BE1025090B1 (es) |
CA (1) | CA3058269A1 (es) |
CO (1) | CO2019011205A2 (es) |
MX (1) | MX2019011619A (es) |
PE (1) | PE20191839A1 (es) |
WO (1) | WO2018178375A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020079274A1 (en) | 2018-10-19 | 2020-04-23 | Univercells S.A. | Method for decontaminating a biomolecule production system and a system suitable for decontamination |
TW202112799A (zh) * | 2019-06-05 | 2021-04-01 | 美商建南德克公司 | 過載層析管柱之再生方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5886154A (en) * | 1997-06-20 | 1999-03-23 | Lebing; Wytold R. | Chromatographic method for high yield purification and viral inactivation of antibodies |
KR101241486B1 (ko) * | 2007-10-30 | 2013-03-15 | 제넨테크, 인크. | 양이온 교환 크로마토그래피에 의한 항체 정제 |
-
2017
- 2017-03-30 BE BE2017/5211A patent/BE1025090B1/fr active IP Right Grant
-
2018
- 2018-03-30 WO PCT/EP2018/058365 patent/WO2018178375A1/en unknown
- 2018-03-30 KR KR1020197031121A patent/KR20190135495A/ko unknown
- 2018-03-30 US US16/499,355 patent/US20210095261A1/en active Pending
- 2018-03-30 EP EP18713974.6A patent/EP3601315A1/en active Pending
- 2018-03-30 MX MX2019011619A patent/MX2019011619A/es unknown
- 2018-03-30 CA CA3058269A patent/CA3058269A1/en active Pending
- 2018-03-30 PE PE2019001971A patent/PE20191839A1/es unknown
-
2019
- 2019-10-08 CO CONC2019/0011205A patent/CO2019011205A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
WO2018178375A1 (en) | 2018-10-04 |
BE1025090B1 (fr) | 2018-10-29 |
EP3601315A1 (en) | 2020-02-05 |
BE1025090A1 (fr) | 2018-10-24 |
PE20191839A1 (es) | 2019-12-31 |
KR20190135495A (ko) | 2019-12-06 |
CO2019011205A2 (es) | 2020-01-17 |
CA3058269A1 (en) | 2018-10-04 |
US20210095261A1 (en) | 2021-04-01 |
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