LU500729B1

LU500729B1 - Woody mosquito-repellent and insect-repellent compound essential oil and preparation method thereof

Info

Publication number: LU500729B1
Application number: LU500729A
Authority: LU
Inventors: Wanxi Peng
Original assignee: Univ Henan Agricultural
Priority date: 2021-10-13
Filing date: 2021-10-13
Publication date: 2022-04-13

Abstract

The present disclosure provides a woody mosquito-repellent and insect-repellent compound essential oil, which includes a compound plant extract and a solvent. The compound plant extract is a mixture of extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf; the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5. The woody mosquito-repellent and insect-repellent compound essential oil of the present disclosure does not contain chemical insecticidal ingredients and preservatives, and all ingredients are plant materials, thus being environment-friendly and free from pollution, and having a remarkable mosquito- repellent effect; also, the present disclosure can remove peculiar smells and leave a faint scent, can be carried outside for use or used as decoration, without limitation in use; furthermore, a fragrant scent can improve the ambient air quality while expelling mosquitoes.

Description

BL-5310 WOODY MOSQUITO-REPELLENT AND INSECT-REPELLENT COMPOUND 500729

ESSENTIAL OIL AND PREPARATION METHOD THEREOF TECHNICAL FIELD

[01] The present disclosure belongs to the technical field of insect repellents, and particularly relates to a woody mosquito-repellent and insect-repellent compound essential oil and a preparation method thereof.

BACKGROUND ART

[02] Hazards of mosquitoes are well known. Not only will it be itchy after being bitten by mosquitoes, but more seriously, mosquitoes can spread diseases such as malaria, filariasis, epidemic encephalitis b, and dengue fever. Therefore, a good mosquito-repellent and insect- repellent product is particularly important.

[03] Mosquitoes are mainly targeted at human odors, such as exhaled carbon dioxide or volatiles on the body surface. Mosquito repellents are sprayed as a barrier on the body that interferes with the induction of chemoreceptors of mosquito antennae against volatiles on the human body surface, so that people are protected from the bites of mosquitoes. There are four common mosquito repellents: DEET, lemon eucalyptus oil, icaridin, and ethyl butylacetylaminopropionate. DEET is a widely used insecticide that is sprayed on the skin or clothing to prevent insect bites by 1-octen-3-ol that blocks the insect olfactory receptor, but because of high skin irritation, DEET cannot be used directly in contact with the skin. Lemon eucalyptus oil is an extract of an Australian eucalyptus. Studies have shown that lemon eucalyptus oil has shorter maximum protection time against mosquitoes than DEET. Mosquito repellents containing lemon eucalyptus oil are generally contraindicated in children younger than 3 years. Icaridin is also known as Picaridin. Its single or compound preparation does not cause damage to common house furnishing materials, including plastics, coatings, decorative metal foils, and paints, and is more effective than DEET. Ethyl butylacetylaminopropionate, also known as Imering, is a broad-spectrum and highly effective insect repellent. It has excellent repellent effects on mosquitoes, flies, and louses. It has a long-lasting repellent effect and can be used under different climatic conditions. The use of certain plants with special scents to repel mosquitoes has a long history, which has documented in the Book of Songs and other literature. The remarkable advantages of plant repellents are green safety and no harm to the human body. However, the existing plant mosquito repellents are short-lasting. 1

BL-5310 SUMMARY LU500729

[04] To solve the problem that plant mosquito repellents have a short acting duration in the prior art, one objective of the present disclosure is to provide a woody mosquito-repellent and insect-repellent compound essential oil.

[05] Another objective of the present disclosure is to provide a method for preparing the foregoing essential oil.

[06] To achieve the above objectives, the present disclosure adopts the following technical solutions:

[07] A woody mosquito-repellent and insect-repellent compound essential oil is provided, including the following components:

[08] 0.5-30 parts by weight of a compound plant extract, and

[09] 80-120 parts by weight of a solvent, where

[10] the compound plant extract is a mixture of extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, and the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5.

[11] Preferably, in the compound plant extract, the extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf may have a mass ratio of 1:1:1:1:1:1:(1-10):(1-10):(1- 10):(1-10):(1-10).

[12] A method for preparing a woody mosquito-repellent and insect-repellent compound essential oil is provided, including the steps of:

[13] step 1, pulverizing fresh argy wormwood leaves, grapefruit flowers, orange flowers, Mozzie busters, peppermints, jasmines, geraniums, lavenders, Roridula, pitcher plants, and walnut leaves, respectively;

[14] step 2, conducting a first extraction on the raw materials pulverized in step 1 with petroleum ether to obtain petroleum ether extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively;

[15] step 3, conducting a second extraction on petroleum ether extracted raw materials in step 2 with ethanol to obtain ethanol extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively; and

[16] step 4, mixing each petroleum ether extract of step 2 and each ethanol extract of step 3 well after removing extractants, to obtain the compound plant extract; dissolving the compound 2

BL-5310 plant extract in a solvent to obtain the woody mosquito-repellent and insect-repellent compound 500729 essential oil.

[17] Preferably, the first extraction in step 2 may be conducted at 30-50°C for 1-3 h.

[18] Preferably, the second extraction in step 3 may be conducted at 60-90°C for 2-4 h.

[19] Preferably, in step 4, the extractants may be removed at a temperature of 1-40°C and a pressure of 0.01-0.99 MPa.

[20] Preferably, the solvent in step 4 may be an alcohol having a concentration of 60-80% by weight.

[21] The grapefruit flower has whitening, brightening, moisturizing, nourishing, bactericidal, and anti-inflammatory effects on the skin, which is an exceptional high-quality raw material for manufacturing organic plant series skin care products.

[22] In the preparation method, a part of the volatile organic compounds are extracted from the raw materials by petroleum ether at a low temperature; then, organic matters in the crude materials are further extracted by ethanol; and finally, water is used to extract water-soluble substances from crude materials extracted by petroleum ether and ethanol. A combination of the three solvents is used in the extraction method from low temperature to high temperature. In one aspect, the volatilization and failure of active ingredients in the crude materials are avoided; in another aspect, and the extraction is more complete and thorough, and the utilization rate of the raw materials is higher than that of the single-solvent extraction and double-solvent extraction.

[23] The present disclosure has the following beneficial effects:

[24] 1. The woody mosquito-repellent and insect-repellent compound essential oil of the present disclosure does not contain chemical insecticidal ingredients and preservatives, and all ingredients are plant materials, thus being environment-friendly and free from pollution, and having a remarkable mosquito-repellent effect; also, the present disclosure can remove peculiar smells and leave a faint scent; and

[25] 2. The woody mosquito-repellent and insect-repellent compound essential oil of the present disclosure is convenient to carry, which can be carried outside for use or used as decoration, without limitation in use; furthermore, a fragrant scent can improve the ambient air quality while expelling mosquitoes.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[26] Specific examples of the present disclosure are described below, and the technical solutions of the present disclosure will be further described in conjunction with the examples, but the present disclosure is not limited thereto.

[27] Example 1 3

BL-5310

[28] A woody mosquito-repellent and insect-repellent compound essential oil was provided 9729 including the following components:

[29] 0.5 parts by weight of a compound plant extract, and

[30] 80 parts by weight of a solvent,

[31] the compound plant extract is a mixture of extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, and the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5.

[32] Preferably, in the compound plant extract, the extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf had a mass ratio of 1:1:1:1:1:1:1:1:1:1:1.

[33] Example 2

[34] A woody mosquito-repellent and insect-repellent compound essential oil was provided, including the following components:

[35] 30 parts by weight of a compound plant extract, and

[36] 120 parts by weight of a solvent,

[37] the compound plant extract is a mixture of extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, and the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5.

[38] Preferably, in the compound plant extract, the extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf had a mass ratio of 1:1:1:1:1:1:10:10:10:10:10.

[39] Example 3

[40] A woody mosquito-repellent and insect-repellent compound essential oil was provided, including the following components:

[41] 15 parts by weight of a compound plant extract, and

[42] 100 parts by weight of a solvent,

[43] the compound plant extract is a mixture of extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, and the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5.

[44] Preferably, in the compound plant extract, the extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf had a mass ratio of 1:1:1:1:1:1:5:5:5:5:5.

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BL-5310

[45] Example 4 500729

[46] A method for preparing a woody mosquito-repellent and insect-repellent compound essential oil was provided, including the following steps:

[47] step 1, fresh argy wormwood leaves, grapefruit flowers, orange flowers, Mozzie busters, peppermints, jasmines, geraniums, lavenders, Roridula, pitcher plants, and walnut leaves were pulverized, respectively;

[48] step 2, a first extraction was conducted on raw materials pulverized in step 1 with petroleum ether to obtain petroleum ether extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively;

[49] step 3, a second extraction was conducted on petroleum ether extracted raw materials in step 2 with ethanol to obtain ethanol extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively; and

[50] step 4, each petroleum ether extract of step 2 was mixed well with each ethanol extract of step 3 after removing extractants, to obtain the compound plant extract; the compound plant extract was dissolved in a solvent to obtain the woody mosquito-repellent and insect-repellent compound essential oil.

[51] The first extraction in step 2 was conducted at 30-50°C for 1-3 h.

[52] The second extraction in step 3 was conducted at 60-90°C for 2-4 h.

[53] In step 4, the extractants were removed at a temperature of 1-40°C and a pressure of 0.01-

0.99 MPa.

[54] The solvent in step 4 was alcohol, and the alcohol had a concentration of 60-80% by weight.

[55] Comparative Example 1

[56] This example provided a formula of a woody mosquito-repellent and insect-repellent compound essential oil, which differed from Example 3 in that there was no grapefruit flower extract.

[57] Comparative Example 2

[58] This example provided a formula of a woody mosquito-repellent and insect-repellent compound essential oil, which differed from Example 3 in that there were no lavender extract and Roridula extract.

[59] Effect test

[60] 1. Test materials

[61] Test samples: According to the formulas of Example 3 and Comparative Examples 1 and

BL-5310 LU500729 2, three woody mosquito-repellent and insect-repellent compound essential oils with corresponding numbers were prepared by the method provided in Example 4.

[62] Test insects: Female Culex pipiens pallens aged 3-5 days was selected, which was a sensitive line artificially reared indoors.

[63] 2. Test method: The aeration device method was used. The aeration device was composed of three sections of circular plexiglass tubes with a diameter of 20 mm and two metal wire meshes. The upper and lower sections were 43 cm long, and the middle section was 20 cm long. One metal wire mesh was arranged at either end of the middle-section circular tube, and 100 mosquitoes were released in the middle tube. After the test sample was opened for 1 h, it was placed in the center of the lower circular tube, and the orifice of the lower tube was kept in a ventilated state. At 8 h, the knockdown rate of mosquitoes (to check the number of mosquitoes with belly upwards) was observed, and the test sample was taken out; the 24-h mortality of mosquitoes was observed under rearing conditions. The test was repeated three times and a blank control was set.

[64] 3. Test results: The test results are shown in the table below.

[65] Table 1 The repellent and knockdown effects of the woody mosquito-repellent and insect- repellent compound essential oil on C. pipiens pallens 8 h Knockdown rate 24 h No Number of mosquitoes Number of Mortality observed umber o (%) 0 mosquitoes (%) Comparative Example 1 Comparative Example 2

[66] The test results of the test method demonstrate that the woody mosquito-repellent and insect-repellent compound essential oil of the present disclosure has an excellent fumigation effect on C. pipiens pallens.

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Claims

BL-5310 WHAT IS CLAIMED IS: 500729

1. A woody mosquito-repellent and insect-repellent compound essential oil, comprising the following components:

0.5-30 parts by weight of a compound plant extract, and 80-120 parts by weight of a solvent, wherein the compound plant extract is a mixture of extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, and the extract is a mixture of petroleum ether extract and ethanol extract in a mass ratio of 1:0.5.

2. The woody mosquito-repellent and insect-repellent compound essential oil according to claim 1, wherein in the compound plant extract, the extracts from argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf have a mass ratio of 1:1:1:1:1:1:(1-10):(1-10):(1-10):(1-10):(1-10).

3. A method for preparing the woody mosquito-repellent and insect-repellent compound essential oil according to claim 1 or 2, comprising the steps of: step 1, pulverizing fresh argy wormwood leaves, grapefruit flowers, orange flowers, Mozzie busters, peppermints, jasmines, geraniums, lavenders, Roridula, pitcher plants, and walnut leaves, respectively; step 2, conducting a first extraction on the raw materials pulverized in step 1 with petroleum ether to obtain petroleum ether extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively; step 3, conducting a second extraction on petroleum ether extracted raw materials in step 2 with ethanol to obtain ethanol extracts of argy wormwood leaf, grapefruit flower, orange flower, Mozzie buster, peppermint, jasmine, geranium, lavender, Roridula, pitcher plant, and walnut leaf, respectively; and step 4, mixing each petroleum ether extract of step 2 and each ethanol extract of step 3 well after removing extractants, to obtain the compound plant extract; dissolving the compound plant extract in a solvent to obtain the woody mosquito-repellent and insect-repellent compound essential oil.

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BL-5310 LU500729

4. The method for preparing the woody mosquito-repellent and insect-repellent compound essential oil according to claim 3, wherein the first extraction in step 2 is conducted at 30-50°C for 1-3 h.

5. The method for preparing the woody mosquito-repellent and insect-repellent compound essential oil according to claim 3, wherein the second extraction in step 3 1s conducted at 60-90°C for 2-4 h.

6. The method for preparing the woody mosquito-repellent and insect-repellent compound essential oil according to claim 3, wherein in step 4, the extractants are removed at a temperature of 1-40°C and a pressure of 0.01-0.99 MPa.

7. The method for preparing the woody mosquito-repellent and insect-repellent compound essential oil according to claim 3, wherein the solvent in step 4 1s an alcohol having a concentration of 60-80% by weight.

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