KR970074932A - Method for preparing human interleukin-6 using yeast - Google Patents
Method for preparing human interleukin-6 using yeast Download PDFInfo
- Publication number
- KR970074932A KR970074932A KR1019960018347A KR19960018347A KR970074932A KR 970074932 A KR970074932 A KR 970074932A KR 1019960018347 A KR1019960018347 A KR 1019960018347A KR 19960018347 A KR19960018347 A KR 19960018347A KR 970074932 A KR970074932 A KR 970074932A
- Authority
- KR
- South Korea
- Prior art keywords
- yeast
- expression plasmid
- gene
- pyhil
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5412—IL-6
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 인체에 존재하는 사이토카인의 일종으로서 인체의 면역반응등과 관련하여 매우 중요한 작용을 하는 물질인 인터루킨-6 (Interleukine-6)의 유전자를 포함하는 효모 발현 플라스미드 및 이를 이용하여 효모에서 인터루킨-6를 제조하는 방법에 관한 것이다.The present invention is a type of cytokine present in the human body, and a yeast expression plasmid containing a gene of interleukine-6, which is a substance which plays a very important role in relation to the immune response of the human body, and an interleukin in yeast using the same. It relates to a method for producing -6.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.
제1도는 IL-6 유전자가 포함된 효모 발현 플라스미드, pYHIL-10Zd를 제조하는 과정을 나타낸 것이다, 제2도는 IL-6 유전자가 포함된 효모 발현 플라스미드, pYHIL-FZd를 제조하는 과정을 나타낸 것이다, 제3도는 본 발명의 발현 플라스미드로 형질전환된 효모 균주가 IL-6를 생산하는 정도를 SDS-폴리아크릴아미드겔 전기영동 및 이의 웨스턴 블럿 방법으로 나타낸 것이다, 제5도는 효모에서 발현되는 재조합 IL-6-lacZ 융합단백질의 IL-6 활성도를 나타낸 것이다.FIG. 1 shows a process for preparing a yeast expression plasmid containing IL-6 gene, pYHIL-10Zd, and FIG. 2 shows a process for preparing a yeast expression plasmid containing IL-6 gene, pYHIL-FZd. Figure 3 shows the degree of production of IL-6 by the yeast strain transformed with the expression plasmid of the present invention by SDS-polyacrylamide gel electrophoresis and its Western blot method, Figure 5 shows the recombinant IL- expressed in yeast IL-6 activity of 6-lacZ fusion protein is shown.
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960018347A KR100204504B1 (en) | 1996-05-28 | 1996-05-28 | Process for the production of human interleukin-6 using yeast |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960018347A KR100204504B1 (en) | 1996-05-28 | 1996-05-28 | Process for the production of human interleukin-6 using yeast |
Publications (2)
Publication Number | Publication Date |
---|---|
KR970074932A true KR970074932A (en) | 1997-12-10 |
KR100204504B1 KR100204504B1 (en) | 1999-06-15 |
Family
ID=19459976
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019960018347A KR100204504B1 (en) | 1996-05-28 | 1996-05-28 | Process for the production of human interleukin-6 using yeast |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR100204504B1 (en) |
-
1996
- 1996-05-28 KR KR1019960018347A patent/KR100204504B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR100204504B1 (en) | 1999-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE169956T1 (en) | DNA CODING FOR PROTEINS THAT BIND HUMAN IL-1 | |
KR840001837A (en) | Method of producing immune interferon in human body | |
ATE77104T1 (en) | QUANTITY AND METHOD FOR MAKING HIGH YIELD RECOMBINATION PRODUCTS. | |
ATE426024T1 (en) | C-C CHEMOKINE RECEPTOR 3: CKR-3 OR EOS-L2 | |
ES2056799T3 (en) | CODANT DNA MOLECULES FOR THE FMDH CONTROL AND STRUCTURAL GENE REGIONS FOR A PROTEIN THAT HAS AN FMDH ACTIVITY AND ITS USE. | |
DE10075011I2 (en) | Receptors for tumor necrosis factor alpha and beta. | |
ES2059428T3 (en) | METHOD FOR THE PREPARATION OF ALIEN PROTEINS IN YEAST, RECOMBINANT, TRANSFORMING DNA. | |
KR840004781A (en) | Human tissue plasminogen activator | |
Yasueda et al. | High-level direct expression of semi-synthetic human interleukin-6 in Escherichia coli and production of N-terminus met-free product | |
KR840008697A (en) | Method for Synthesis, Secretion and Expression Media of Heterologous Proteins Using Homologous Yeast Signals | |
GB8616219D0 (en) | Monoclonal antibodies | |
KR880009991A (en) | Polypeptide with Anticoagulant Action | |
ATE282691T1 (en) | CHEMOKINE TYPE CC-LIKE PROTEIN | |
DE3850774D1 (en) | BEEF INTERLEUKIN 1 ALPHA. | |
JPS6485098A (en) | Glycoprotein and production thereof | |
KR970074932A (en) | Method for preparing human interleukin-6 using yeast | |
CA2032167A1 (en) | Dna coding for protein binds to enhancer of.alpha.-fetoprotein gene | |
GB2171703B (en) | Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re | |
AUPM772494A0 (en) | Improvements in production of proteins in host cells | |
ES2056785T3 (en) | METHOD OF USE OF BAR1 FOR THE SECRETION OF FOREIGN PROTEINS. | |
EP0171000A3 (en) | Process for producing heterologous matured protein or peptide | |
ES2012283A6 (en) | Endotoxin muteins | |
Nishimura et al. | Role of leucine residues in the C‐terminal region of human interleukin‐6 in the biological activity | |
Ramanathan et al. | Multiple forms of recombinant murine interleukin-4 expressed in COS-7 monkey kidney cells | |
EP0282512A4 (en) | Messenger rna stabilization in animal cells. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20060330 Year of fee payment: 8 |
|
LAPS | Lapse due to unpaid annual fee |