KR970027104A - 펩타이드 또는 단백질을 포함하는 생물학적 매트리스의 한외여과 방법 - Google Patents
펩타이드 또는 단백질을 포함하는 생물학적 매트리스의 한외여과 방법 Download PDFInfo
- Publication number
- KR970027104A KR970027104A KR1019960056619A KR19960056619A KR970027104A KR 970027104 A KR970027104 A KR 970027104A KR 1019960056619 A KR1019960056619 A KR 1019960056619A KR 19960056619 A KR19960056619 A KR 19960056619A KR 970027104 A KR970027104 A KR 970027104A
- Authority
- KR
- South Korea
- Prior art keywords
- proteins
- membranes
- membrane
- ultrafiltration
- containing peptides
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
본 발명은 펩타이드 또는 단백질을 포함하는 복합 생물학적 매트리스, 특히 발효 브로쓰를 한외여과를 통해 예비정제하는 방법에 관한 것이다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (7)
- 규정된 막의 분리 한계가 막에 의해 보유되는 펩타이드 또는 단백질 분자량의 2 내지 5배인 막 상에서 한외여과를 통해 펩타이드 또는 단백질을 포함하는 세포-비함유 배양 브로쓰를 예비정제하는 방법
- 제1항에 있어서, 막의 규정된 분리 한계가 막에 의해 보유되는 팝타이드 또는 단백질의 분자량의 3 내지 4배인 방법.
- 제1항 또는 제2항에 있어서, 펩타이드가 히루딘인 방법.
- 제3항에 있어서, 막의 분리 한계가 20 내지 30kD인 방법.
- 제1항 내지 제4항 중 어느 한 항에 있어서, 여과의 전체 과정에 걸쳐 특정 투과액 유동 속도가 10 내지 35 1/㎡/h인 방법.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 옥수수 침지액이 사용되는 경우, 배양 브로쓰의 온도가 5 내지 15℃인 방법.
- 제1항 내지 제6항 중 어느 한 항에 있어서, 투과액 중의 생성물 농도가 일정값을 취할때까지 투과액을 재순환시키는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19543737.3 | 1995-11-24 | ||
DE19543737A DE19543737A1 (de) | 1995-11-24 | 1995-11-24 | Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices |
Publications (1)
Publication Number | Publication Date |
---|---|
KR970027104A true KR970027104A (ko) | 1997-06-24 |
Family
ID=7778254
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019960056619A KR970027104A (ko) | 1995-11-24 | 1996-11-22 | 펩타이드 또는 단백질을 포함하는 생물학적 매트리스의 한외여과 방법 |
Country Status (7)
Country | Link |
---|---|
US (1) | US6103502A (ko) |
EP (1) | EP0775710A1 (ko) |
JP (1) | JP3218193B2 (ko) |
KR (1) | KR970027104A (ko) |
AU (1) | AU726264B2 (ko) |
CA (1) | CA2191023A1 (ko) |
DE (1) | DE19543737A1 (ko) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10108211A1 (de) * | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen |
US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
WO2004042012A2 (en) * | 2002-11-01 | 2004-05-21 | Bayer Healthcare Llc | Process for concentration of macromolecules |
US20060051347A1 (en) | 2004-09-09 | 2006-03-09 | Winter Charles M | Process for concentration of antibodies and therapeutic products thereof |
CN102065870B (zh) * | 2008-04-16 | 2014-11-19 | 比奥根艾迪克Ma公司 | 使用聚亚烷基二醇和过渡金属分离生物大分子的方法 |
US9155775B1 (en) | 2015-01-28 | 2015-10-13 | Teva Pharmaceutical Industries, Ltd. | Process for manufacturing glatiramer acetate product |
CN107325177A (zh) * | 2017-09-01 | 2017-11-07 | 荆州市民康生物科技有限公司 | 一种提高菲牛蛭素提取收率的方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE255943C (ko) * | ||||
IT1157340B (it) | 1982-11-29 | 1987-02-11 | F I M A | Dispositivo per collegare amovibilamente utensili abrasivi all'albero di levigatrici e simili |
CA1341414C (fr) | 1984-03-27 | 2002-12-31 | Paul Tolstoshev | Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine |
DE3438296A1 (de) | 1984-04-18 | 1985-11-07 | Hoechst Ag, 6230 Frankfurt | Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel |
ATE64956T1 (de) * | 1984-06-14 | 1991-07-15 | Ciba Geigy Ag | Verfahren zur herstellung von thrombininhibitoren. |
DE3429430A1 (de) | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens |
DE3445517C2 (de) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins |
DE3506992A1 (de) | 1985-02-27 | 1986-08-28 | Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn | Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten |
ES2055149T3 (es) | 1985-04-11 | 1994-08-16 | Hoechst Ag | Derivado de hirudina. |
FR2593518B1 (fr) | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
EP0209061B1 (de) | 1985-07-17 | 1994-01-12 | Hoechst Aktiengesellschaft | Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel |
DD255943A1 (de) * | 1986-11-10 | 1988-04-20 | Akad Wissenschaften Ddr | Verfahren zur konzentrierung und reinigung von insulin und seinen derivaten |
GB8723651D0 (en) * | 1987-10-08 | 1987-11-11 | Express Foods Group Ltd | Process for containing concentrates |
AU604925B2 (en) * | 1988-02-23 | 1991-01-03 | Schering Aktiengesellschaft | A hirudin derivative |
DE4140381A1 (de) * | 1991-12-07 | 1993-06-09 | Hoechst Ag, 6230 Frankfurt, De | Neue synthetische isohirudine mit verbesserter stabilitaet |
DE4302163A1 (de) * | 1993-01-27 | 1994-07-28 | Thomae Gmbh Dr K | Verfahren zur Reinigung von Proteinen und Peptiden |
US5468844A (en) * | 1994-01-07 | 1995-11-21 | Protose Technologies Inc. | Process for the membrane-filtering of protein solutions |
-
1995
- 1995-11-24 DE DE19543737A patent/DE19543737A1/de not_active Ceased
-
1996
- 1996-11-11 EP EP96118021A patent/EP0775710A1/de not_active Ceased
- 1996-11-22 AU AU71932/96A patent/AU726264B2/en not_active Ceased
- 1996-11-22 KR KR1019960056619A patent/KR970027104A/ko not_active Application Discontinuation
- 1996-11-22 JP JP31154296A patent/JP3218193B2/ja not_active Expired - Fee Related
- 1996-11-22 CA CA002191023A patent/CA2191023A1/en not_active Abandoned
- 1996-11-22 US US08/755,114 patent/US6103502A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2191023A1 (en) | 1997-05-25 |
JP3218193B2 (ja) | 2001-10-15 |
AU7193296A (en) | 1997-05-29 |
JPH09173793A (ja) | 1997-07-08 |
AU726264B2 (en) | 2000-11-02 |
US6103502A (en) | 2000-08-15 |
DE19543737A1 (de) | 1997-05-28 |
EP0775710A1 (de) | 1997-05-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR880700640A (ko) | 펩타이드 제제와 이것의 제조방법 및 용도 | |
Bishop et al. | Isolation and identification of the fast-death factor in Microcystis aeruginosa NRC-1 | |
FUJIMAKI et al. | Applying proteolytic enzymes on soybean. 3. Diffusable bitter peptides and free amino acids in peptic hydrolyzate of soybean protein | |
Kondo et al. | Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties | |
Lilley et al. | Purification of the insecticidal toxin in crystals of Bacillus thuringiensis | |
KR900009095A (ko) | 합성 항원, 이의 제조방법 및 용도 | |
SE7712846L (sv) | Flytande fodoemnesprodukt innehallande sojaproteinmembranisolat | |
ZA927993B (en) | A colostrum fraction, a process of preparing it and its use. | |
KR970027104A (ko) | 펩타이드 또는 단백질을 포함하는 생물학적 매트리스의 한외여과 방법 | |
ATE83248T1 (de) | Auf polyamid oder zellulosehydrat immobilisierte proteine und ihre verwendung zur herstellung von biokatalysatoren, teststreifen oder chromatographiematerialien. | |
RU2218181C2 (ru) | ЧЕЛОВЕЧЕСКИЙ СЕМАФОРИН L(H-Sema-L) И СООТВЕТСТВУЮЩИЕ СЕМАФОРИНЫ В ДРУГИХ ВИДАХ | |
Visser et al. | Action of plasmin on bovine β-casein in a membrane reactor | |
US5698416A (en) | Methods for production of antigens under control of temperature-regulated promotors in enteric bacteria | |
Dimond et al. | Purification and characterization of a staphylococcal epidermolytic toxin | |
ATE82471T1 (de) | Verfahren zur abtrennung bestimmter proteine aus molke. | |
KR950000889A (ko) | 신규 아미노펩티다제 | |
Alouf et al. | [8] Production, purification, and assay of streptolysin O | |
Ikigai et al. | Simplified method for preparation of concentrated exoproteins produced by Staphylococcus aureus grown on surface of cellophane bag containing liquid medium | |
Blomqvist et al. | Characterization of domain borders and of a naturally occurring major fragment of staphylococcal α‐toxin | |
Bar | Phase toxicity in multiphase biocatalysis | |
Awad Jr et al. | Self purification of two serine endopeptidases | |
DE59109155D1 (de) | Verfahren zur herstellung von vakzinen und ihre verwendung | |
KR920016080A (ko) | 화장료 | |
KR970021283A (ko) | 발효 브로쓰의 처리방법 | |
Gessler | A new scaleable method for the purification of botulinum neurotoxin type E |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
N231 | Notification of change of applicant | ||
A201 | Request for examination | ||
N231 | Notification of change of applicant | ||
E902 | Notification of reason for refusal | ||
E601 | Decision to refuse application |